Composite plant growth regulator as well as preparation method and application thereof

Abstract

The invention discloses a composite plant growth regulator and a preparation method and application thereof, the composite plant growth regulator is composed of 10-20 parts of effective active components, 80-100 parts of solvent and 8-10 parts of accelerant, the effective active components are brassinolide and cuttlebone extract according to the mass ratio of 1: 300-400; the solvent is water. According to the compound plant growth regulator, the effective active ingredients and the accelerant are combined according to a proper proportion, so that the growth and development of wheat can be remarkably promoted, the photosynthesis of plants is enhanced, the lodging resistance of crops is improved, and finally the yield and quality of wheat are improved; meanwhile, the use of chemical synthetic growth regulators can be effectively reduced, and the composition is safer, more environmentally friendly and low in drug resistance risk.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically relates to a composite plant growth regulator and a preparation method and application thereof. Background Art

[0002] Wheat is one of the important food crops in my country. It plays an important role in my country's food security and also plays a pivotal role in my country's economic development. The discovery and application of dwarf genes in the second half of the 20th century not only reduced the sensitivity of wheat to lodging, but also improved the harvest index and increased wheat yield. Modern breeding technology can also further reduce the plant height of wheat. However, while further reducing the plant height improves the lodging resistance, it will also lead to smaller leaves, poor field ventilation and light transmission, affecting crop photosynthesis, and thus reducing yield. Therefore, how to reduce wheat lodging under high-yield and super-high-yield conditions and achieve stable yield is a difficult problem. Lodging is closely related to the external environment of wheat growth, management measures, growth and development process, and variety characteristics. The use of growth regulators is one of the effective ways to improve the lodging resistance of crops. At present, the plant growth regulators used to improve the lodging resistance of crops are mainly chemically synthesized growth regulators, which are mainly divided into two categories: one is gibberellin synthesis inhibitors, such as paclobutrazol; the other is ethylene releasers, such as ethephon. Although chemically synthesized growth regulators are effective in improving wheat's resistance to lodging, long-term use of such chemical regulators may have potential impacts on the environment and human health. For example, they may cause soil residues and affect soil microbial communities, and long-term use of the same chemical regulator may cause plants or pathogens to develop resistance, reducing the effectiveness of the regulator. It may also have a negative impact on non-target organisms (such as beneficial insects) and disrupt the ecological balance. Plant-derived growth regulators have significant advantages in environmental friendliness, safety, low resistance risk, safety for beneficial organisms, versatility, and eco-friendliness in applications such as wheat lodging resistance. They are ideal substitutes for chemical regulation regulators. Therefore, how to prepare a composite plant growth regulator with higher safety and stable effects has become a technical problem that needs to be solved urgently. Summary of the invention

[0003] The object of the present invention is to provide a composite plant growth regulator, which can be used in wheat planting to significantly promote wheat growth and development, enhance plant photosynthesis, improve crop lodging resistance, and ultimately improve wheat yield and quality.

[0004] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A composite plant growth regulator is composed of 10-20 parts of effective active ingredients, 80-100 parts of solvents and 8-10 parts of promoters. The effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:300-400; the solvent is water.

[0005] Preferably, the accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2-0.5:1.

[0006] Preferably, the cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder and deionized water evenly according to the solid-liquid ratio, adjust the pH of the solution to 5.5-6.0, add 1.5wt% complex enzyme solution, perform enzymolysis at 50°C for 2h, increase the temperature to 55°C and adjust the pH to 6.5-7.5, then add 3wt% pectinase and perform enzymolysis for 2h, then add 1wt% chitosanase and perform enzymolysis at 40-45°C for 3-4h, inactivate the enzyme at high temperature to obtain enzymolysis solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol according to the solid-liquid ratio, and then ultrasonically dispersed for 30 minutes, heated and stirred at 60°C for reflux reaction for 1 hour, filtered, and the filtrate B was taken. The filtrate was centrifuged at 5000 rpm for 10 minutes to obtain the supernatant to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0007] Preferably, in step (1), the material-liquid ratio of cuttlefish powder to deionized water is 1 g:30 ml.

[0008] Preferably, the complex enzyme solution in step (1) contains 5-10 g / L cellulase and 10-15 g / L flavor protease.

[0009] Preferably, the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the flavor protease is 50,000 U / g.

[0010] Preferably, the enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0011] Preferably, in step (2), the solid-liquid ratio of the residue A to 80% ethanol is 1 g:10 ml.

[0012] The present invention also provides a method for preparing the above-mentioned composite plant growth regulator, comprising the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0013] The composite plant growth regulator prepared by the present invention can be used to promote wheat growth and improve wheat lodging resistance. When used, it needs to be diluted 8-10 times before spraying, and the dosage per mu is 10L. It can be sprayed during various growth periods of wheat, such as tillering stage, rising and jointing stage, booting stage, and filling stage.

[0014] The cuttlebone extract prepared by the present invention utilizes a variety of enzymes to perform decomposition under different conditions, and decomposes the components in the cuttlebone to the maximum extent. The obtained product is rich in a variety of small molecule biologically active components, such as polysaccharides, amino acids, organic acids, etc. The multiple active components work together to promote nutrient absorption and utilization, promote wheat root growth, enhance the anchoring ability of plants, and also enhance the tolerance of plants to environmental stress; in addition, the calcium salt component in the cuttlebone extract can also enhance the stability of plant cell walls, promote the thickening and strengthening of cell walls, and can make the stems of wheat thicker and the plants dwarfed, thereby effectively improving the lodging resistance of wheat.

[0015] Brassinolide is a natural plant growth regulator that can significantly promote plant growth and development, improve photosynthesis efficiency, and enhance plant stress resistance. The combination of brassinolide and cuttlebone extract can work synergistically to further enhance wheat's resistance to lodging; brassinolide regulates plant endogenous hormones to promote the thickening of cell walls and make the stems thicker; while the active ingredients in cuttlebone extract can further regulate the plant's immune response and enhance the overall health of the plant. The combination of the two can significantly improve wheat's stress resistance, allowing it to maintain a good growth state in the face of adverse environmental conditions such as drought and salinity.

[0016] The promoter used in the present invention contains two components, taurine and methyltriethoxysilane, wherein taurine can improve the permeability of plant cell membranes, help plants absorb brassinolide and cuttlebone extract, and also participate in regulating biochemical reactions in plants, thereby improving the adaptability of plants to adversity. Methyltriethoxysilane and taurine act synergistically to further enhance the permeability of plant cell membranes, enhance the penetration ability of the regulator on the leaf surface, help brassinolide and cuttlebone extract enter the plant body faster to exert their effects, and improve the absorption efficiency of the regulator. The two complement each other and are indispensable.

[0017] The beneficial effects of the present invention are as follows: the prepared composite plant growth regulator has a significant effect on improving the lodging resistance of wheat plants in wheat planting, can significantly inhibit the elongation of wheat internodes, dwarf plant height, make seedlings uniform and strong, and improve the lodging resistance of plants; increase the photosynthesis rate of leaves, promote the development of wheat ears, and improve wheat yield and quality; effectively reduce the use of chemical synthetic growth regulators, are safer and more environmentally friendly, and have a low risk of drug resistance. DETAILED DESCRIPTION

[0018] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0019] Example 1 A composite plant growth regulator is composed of 10kg of effective active ingredients, 80kg of solvent and 8kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:300; and the solvent is water.

[0020] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0021] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0022] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0023] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0024] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0025] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0026] Example 2 A composite plant growth regulator is composed of 20kg of effective active ingredients, 100kg of solvent and 10kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:350; and the solvent is water.

[0027] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.4:1.

[0028] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 6.0, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 7.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 45°C for 4 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0029] The complex enzyme solution in step (1) contains 10 g / L cellulase and 15 g / L flavor protease.

[0030] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0031] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0032] The present invention also provides a method for preparing the above-mentioned composite plant growth regulator, comprising the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0033] Example 3 A composite plant growth regulator is composed of 15kg of effective active ingredients, 90kg of solvent and 9kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:400; and the solvent is water.

[0034] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.5:1.

[0035] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.8, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 7.0, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 45°C for 4 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0036] The complex enzyme solution in step (1) contains 8 g / L cellulase and 12 g / L flavor protease.

[0037] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0038] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0039] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0040] Comparative Example 1 A composite plant growth regulator comprises 10 kg of effective active ingredients and 80 kg of solvent; the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:300; and the solvent is water.

[0041] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0042] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0043] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0044] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0045] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: Take a solvent and then add the effective active ingredient obtained in step S1 into it, high-pressure homogenize for 3-5 minutes, sterilize and then package to obtain the final product.

[0046] This comparative example is basically the same as Example 1, except that it does not contain a promoter component.

[0047] Comparative Example 2 A composite plant growth regulator is composed of 10 kg of brassinolide, 80 kg of water and 8 kg of an accelerator, wherein the accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0048] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: Add brassinolide and accelerator to the solvent, stir evenly, and high pressure homogenize for 3-5 minutes. After sterilization, divide the package to obtain the final product.

[0049] This comparative example is basically the same as Example 1, except that it does not contain the cuttlebone extract and its preparation method.

[0050] Comparative Example 3 A composite plant growth regulator is composed of 10 kg of cuttlebone extract, 80 kg of water and 8 kg of accelerator, wherein the accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0051] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0052] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0053] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0054] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0055] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing cuttlebone extract; S2: adding the accelerator to the solvent, stirring evenly, then adding the above-obtained cuttlebone extract therein, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0056] This comparative example is basically the same as Example 1, except that it does not contain brassinolide.

[0057] Comparative Example 4 A composite plant growth regulator is composed of 10kg of effective active ingredients, 80kg of solvent and 8kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:100; and the solvent is water.

[0058] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0059] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0060] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0061] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0062] The enzyme activity of the pectinase is 50,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0063] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0064] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0065] Comparative Example 5 A composite plant growth regulator is composed of 10kg of effective active ingredients, 80kg of solvent and 8kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:200; and the solvent is water.

[0066] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0067] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0068] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0069] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0070] The enzyme activity of the pectinase is 40,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0071] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0072] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0073] Comparative Example 6 A composite plant growth regulator is composed of 10kg of effective active ingredients, 80kg of solvent and 8kg of promoter, wherein the effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:500; and the solvent is water.

[0074] The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2:1.

[0075] The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder with deionized water at a solid-liquid ratio of 1 g:30 ml, adjust the pH of the solution to 5.5, add 1.5 wt% of the complex enzyme solution, perform enzymatic hydrolysis at 50°C for 2 h, increase the temperature to 55°C and adjust the pH to 6.5, then add 3 wt% of pectinase and perform enzymatic hydrolysis for 2 h, then add 1 wt% of chitosanase and perform enzymatic hydrolysis at 40°C for 3 h, inactivate the enzyme at high temperature to obtain the enzymatic solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol at a solid-liquid ratio of 1 g:10 ml, and then ultrasonically dispersed for 30 min, heated at 60 °C with stirring and reflux for 1 h, filtered, and the filtrate B was collected. The filtrate was centrifuged at 5000 rpm for 10 min, and the supernatant was obtained to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

[0076] The complex enzyme solution in step (1) contains 5 g / L cellulase and 10 g / L flavor protease.

[0077] The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

[0078] The enzyme activity of the pectinase is 50,000 U / g, and the enzyme activity of the chitosanase is 100,000 U / g.

[0079] The preparation method of the above-mentioned composite plant growth regulator comprises the following steps: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

[0080] This comparative example is basically the same as Example 1, except that the mass ratio of brassinolide and cuttlebone extract in the effective active ingredients is different.

[0081] Wheat planting experiment The experimental field was selected from a farmland in Qingyun Town, Linshu County, Linyi City. The annual average temperature is 14.7℃, the annual average precipitation is 866.8 mm, the soil is clay, alkaline, and the fertility is above average. The soil pH is 7.60, the organic matter content is 21.51 g / kg, the total nitrogen content is 1.35 g / kg, the available phosphorus content is 10.66 mg / kg, the available potassium content is 175.21 mg / kg, and the total salt content is 1.42 g / kg. The previous crop is rice. The wheat variety for the test is Linmai 14, which was selected from Linyi Academy of Agricultural Sciences. Wheat was sown mechanically in rows, with a sowing rate of 15.0 kg per mu, a basic seedling number of about 220,000 plants per mu, and 40.0 kg of compound fertilizer (N:P:K=15%:15%:15%, mass fraction, the same below) per mu as basal fertilizer, 5 kg / mu of urea (N content of 46%) as tillering fertilizer, and 5 kg / mu of urea (N content of 46%) as jointing fertilizer and heading fertilizer.

[0082] The experiment set up 10 treatments, namely Examples 1-3, Comparative Examples 1-6 and a blank control test group, and each treatment area was 1 mu. The composite plant growth regulator application device was a drone, and the leaves were sprayed once at the tillering stage, jointing stage, and heading stage of wheat, with a dosage of 5L / mu, diluted 10 times before spraying; the blank control group was not sprayed with any product. 30 days after the first application of the growth regulator, the wheat seedlings were investigated, and each treatment group adopted a diagonal 5-point sampling method, with an area of ​​1m 2 , record the number of tillers; at the maturity stage of wheat, each treatment adopts the diagonal 5-point sampling method, selects 20 representative wheat plants at each point (a total of 100 plants for each treatment), measures the length of the first internode and the second internode at the base, the stem diameter, fresh weight, and dry weight, and measures the bending resistance of the second internode. In addition, investigate the ear length and plant height of wheat in each treatment, and observe the lodging of wheat in the field. The test results are shown in Tables 1-3.

[0083] Table 1 Effects of different treatments with plant growth regulators on wheat traits It can be seen from the contents of Table 1 above that, compared with the blank control group, the composite plant growth regulator prepared by using Examples 1-3 of the present invention can significantly promote wheat tillering, and the length of the first internode and the second internode at the base of wheat is reduced, and the stem thickness is increased, which indicates that the regulator of the present invention can significantly inhibit the elongation of wheat internodes, dwarf plant height, optimize nutrient supply to increase the stem thickness of wheat, and improve the lodging resistance of the plant.

[0084] Table 2 Effects of different treatments with plant growth regulators on fresh weight and dry weight of wheat internodes It can be seen from the contents of Table 2 that, compared with the blank group and the control group, the use of the composite plant growth regulators of Examples 1-3 of the present invention promoted the accumulation of dry matter in wheat internodes.

[0085] Table 3 Effects of different treatments on wheat spike length, plant height and lodging resistance As can be seen from the data in Table 3 above, compared with the blank control group, the plant height and lodging rate of the treatment groups of Examples 1-3 of the present invention were significantly reduced, and the ear length increased, which shows that the use of the composite plant growth regulator of the present invention can dwarf the plant, promote the development of the wheat ear by adjusting the plant nutrient absorption balance, and achieve the purpose of strengthening the seedlings and promoting production. At the same time, the multi-components of the present invention can also promote the growth of the wheat root system, enhance the anchoring ability of the plant, and dwarf the plant, thereby effectively improving the lodging resistance of wheat. As can be seen from the data in Comparative Examples 1-6, by changing the dosage composition and ratio of the raw materials of the present invention, the growth traits of the wheat plants are correspondingly weakened and the lodging resistance is deteriorated.

[0086] Wheat was harvested at maturity, and the wheat yield and yield composition were counted. The specific results are shown in Table 4.

[0087] Table 4 Effects of wheat yield and yield components in different treatment groups From the above table 4, it can be seen that after the composite plant growth regulator of Examples 1-3 of the present invention, the number of wheat ears, number of grains per ear, thousand-mile weight and per-acre yield were significantly higher than those of the blank control group. Although the yield of the treatment groups of Comparative Examples 1-6 was also improved, the overall effect was lower than that of the embodiment, which shows that the formula ratio of the embodiment of the present invention is the optimal dosage ratio, and changing the raw material ratio or composition will reduce the wheat growth promotion effect.

[0088] It should be noted that the above embodiments are only some embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A composite plant growth regulator, which is composed of 10-20 parts of effective active ingredients, 80-100 parts of solvents, and 8-10 parts of promoters, characterized in that: The effective active ingredients are brassinolide and cuttlebone extract in a mass ratio of 1:300-400; and the solvent is water.

2. The composite plant growth regulator according to claim 1, characterized in that: The accelerator is composed of taurine and methyltriethoxysilane in a mass ratio of 0.2-0.5:

1.

3. The composite plant growth regulator according to claim 1, characterized in that: The cuttlebone extract is prepared by the following method: (1) Clean the cuttlebone, dry it and grind it into powder, pass it through an 80-mesh sieve, mix the cuttlebone powder and deionized water evenly according to the solid-liquid ratio, adjust the pH of the solution to 5.5-6.0, add 1.5wt% complex enzyme solution, perform enzymolysis at 50°C for 2h, increase the temperature to 55°C and adjust the pH to 6.5-7.5, then add 3wt% pectinase and perform enzymolysis for 2h, then add 1wt% chitosanase and perform enzymolysis at 40-45°C for 3-4h, inactivate the enzyme at high temperature to obtain enzymolysis solution, and separate by high-speed centrifugation to obtain supernatant A and residue A; (2) Residue A was mixed with 80% ethanol according to the solid-liquid ratio, and then ultrasonically dispersed for 30 minutes, heated and stirred at 60°C for reflux reaction for 1 hour, filtered, and the filtrate B was taken. The filtrate was centrifuged at 5000 rpm for 10 minutes to obtain the supernatant to obtain supernatant B; (3) The supernatant A and the supernatant B are mixed evenly to obtain a cuttlebone extract.

4. The composite plant growth regulator according to claim 3, characterized in that: In step (1), the material-liquid ratio of cuttlefish powder to deionized water is 1 g:30 ml.

5. The composite plant growth regulator according to claim 3, characterized in that: The complex enzyme solution in step (1) contains 5-10 g / L cellulase and 10-15 g / L flavor protease.

6. The composite plant growth regulator according to claim 5, characterized in that: The enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the flavor protease is 50,000 U / g.

7. The composite plant growth regulator according to claim 3, characterized in that: The enzyme activity of pectinase is 40,000 U / g, and the enzyme activity of chitosanase is 100,000 U / g.

8. The composite plant growth regulator according to claim 3, characterized in that: The solid-liquid ratio of the residue A to 80% ethanol in step (2) is 1 g:10 ml.

9. A method for preparing the composite plant growth regulator according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: preparing a cuttlebone extract and mixing it with brassinolide in a certain proportion to obtain an effective active ingredient; S2: adding the accelerator to the solvent, stirring evenly, then adding the effective active ingredient obtained in step S1, high pressure homogenizing for 3-5 minutes, sterilizing and packaging to obtain the final product.

10. Use of the composite plant growth regulator according to any one of claims 1 to 8 for improving lodging resistance in wheat planting.

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