Formula camel milk powder with function of assisting teenagers to grow tall

Abstract

The invention relates to the technical field of preparation of modified milk powder, and discloses formula camel milk powder with a function of assisting teenagers to grow tall, and the formula camel milk powder comprises the following raw materials: camel milk powder, compound minerals, compound vitamins and a compound active composition, the compound mineral substances comprise magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins comprise vitamin B5, vitamin K2 and vitamin K3; the compound active composition comprises radix puerariae polypeptide, fructus gardeniae polypeptide and poria cocos polypeptide; the kudzuvine root polypeptide, the gardenia polypeptide and the poria cocos polypeptide are respectively small molecular peptides obtained by performing enzymolysis on natural kudzuvine root protein, natural gardenia protein and natural poria cocos protein; all the components are from camel milk, trace elements, vitamins and medicinal and edible natural plants which are harmless to a human body, so that the safety is high; all the components have a synergistic effect, so that children and teenagers are supplemented and helped to improve bone health, linear growth is improved, and growth and development are promoted.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of modulated milk powder, in particular to a formula camel milk powder with the function of assisting teenagers to grow taller. Background Art

[0002] During childhood and adolescence, bones grow and develop rapidly, mainly in bone construction, with accelerated bone turnover and a dominant bone formation, so bone mass grows steadily. Bone growth includes linear growth and bone mass accumulation. When exploring the bone health and height development of children and adolescents, it is necessary to consider both bone mass accumulation and linear growth. Among them, linear growth refers to the growth of an organism at a constant rate over a certain period of time. Linear growth can be reflected in multiple physiological indicators, such as weight, height, and the weight of certain organs. During childhood, linear growth is the main growth, with an average growth rate of about 5 to 6 cm per year. During adolescence, especially puberty, bone mass accumulation is the main growth. About 40% to 60% of adult bone mass is achieved during adolescence, and 90% of peak bone mass has been accumulated by the age of 18. Bone mineral content and bone density gradually increase with bone growth, and peak bone mass will be reached in adulthood. Bone mass during childhood and adolescence determines the peak bone mass in a person's lifetime. Factors affecting bone mass include genetic and environmental factors, and nutrition is an important environmental factor affecting peak bone mass.

[0003] According to relevant statistics, the growth retardation rate of children under 6 years old is 8%, while the growth retardation rate of children and adolescents aged 6 to 17 is 7%. The growth retardation rate of urban and rural children and adolescents differs by more than 3 times. Growth retardation is mainly manifested in skeletal retardation, which manifests as linear growth retardation in childhood and linear growth retardation and slow bone accumulation in adolescence, which may lead to osteoporosis. The causes of growth retardation are diverse, involving genetics, nutrition, endocrine, chronic diseases, drugs, psychology and other aspects. Excluding genetic factors, the most important factors affecting growth retardation in children and adolescents are nutrition and endocrine: on the one hand, bone growth and development require nutrients such as calcium and vitamin D, which participate in bone construction as basic components; on the other hand, bone growth and development require endocrine hormones such as growth hormone and sex hormone, which can make chondrocytes proliferate and differentiate to form secondary ossification centers, causing longitudinal growth of long bones.

[0004] Camel milk is rich in various nutrients, including protein, calcium, phosphorus, vitamins (such as A, D, E) and amino acids, which can provide certain nutritional support for the bone growth and development of children and adolescents. However, there is currently a lack of relevant research on camel milk assisting adolescents in growing taller. It is not known whether camel milk can provide comprehensive nutrition for the bone growth and development of children and adolescents, nor is it known whether camel milk has a beneficial effect on the endocrine system of children and adolescents with growth retardation, let alone the synergistic effect of camel milk and other ingredients in the bone growth and development of children and adolescents; for example, patent CN202110979516.5 discloses a milk powder that is beneficial to children's bone growth and development and a preparation method thereof. Its basic ingredients include cow's milk, goat's milk, camel's milk, and yak's milk. Camel milk is only one of them, which mainly plays a role in improving nutrition and flavor, while the vitamins and other substances therein play a role in assisting bone growth and development.

[0005] Therefore, there is an urgent need to study the role of camel milk and related ingredients in bone mass accumulation and linear growth in children and adolescents, so as to obtain a formula camel milk powder that has the function of assisting adolescents to grow taller, to supplement and help children and adolescents improve bone health, enhance linear growth, and promote growth and development. Summary of the invention

[0006] In order to solve the above-mentioned existing problems, the present invention discloses a formula camel milk powder with the function of assisting teenagers to grow taller, including camel milk powder, compound minerals, compound vitamins and compound active compositions. All ingredients are from camel milk, trace elements, vitamins and natural plants that are both medicinal and edible and are harmless to the human body, and have high safety. The ingredients have a synergistic effect, and supplement and help children and adolescents improve bone health, enhance linear growth, and promote growth and development.

[0007] On the one hand, the present invention provides a formula camel milk powder with the function of assisting adolescents to grow taller, comprising the following raw materials: camel milk powder, compound minerals, compound vitamins and compound active compositions; the compound minerals include magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins include vitamin B5, vitamin K2 and vitamin K3; the compound active compositions include kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein, respectively.

[0008] The invention's method of assisting adolescents in growing taller has the following implications: first, promoting the linear growth (height growth) of adolescents without the influence of non-genetic factors; second, promoting the accumulation of bone mass in adolescents, that is, improving the bone health of adolescents while promoting height growth; third, providing sufficient nutrition for the physiological process of adolescents' height growth and activating the physiological pathways related to adolescents' height growth; fourth, further enhancing the overall effect of promoting adolescents' height growth when used in combination with other products that promote adolescents' height growth.

[0009] The core of the camel milk formula of the present invention in assisting adolescents to grow taller is that the active substances, compound minerals, compound vitamins and compound active compositions in camel milk have a synergistic effect. Only when the test mice were gavaged with camel milk powder containing the above formula, the linear growth of 4-6 weeks of age was accelerated, and the bone mass accumulation of 6-8 weeks of age was accelerated. The bone growth and bone mass accumulation mechanism of adolescents are similar to those of mice. Therefore, it can be understood that the camel milk formula of the present invention also has an accelerating effect on the growth of adolescents. Based on a large number of experiments, the inventor speculates that the auxiliary growth mechanism of the camel milk formula of the present invention may be: first, the camel milk formula provides comprehensive nutrition for bone growth and promotes the formation of bone tissue; second, the compound form of trace elements in the compound minerals is a complex relationship between it and other components. The interaction provides a chemical basis, and other ingredients may promote the absorption and bioavailability of trace elements by forming coordination with trace elements; third, the products of metabolic transformation of compound vitamins in the body may provide a carrier or other similar effects on the compound active composition, assisting the relevant ingredients in the formula to bind to bone growth-related targets; fourth, the compound active composition includes several small molecule peptides obtained by enzymatic hydrolysis, and reducing the molecular weight is beneficial to improving the absorption rate and bioavailability; fifth, the synergistic effect between the components may be that they act on the upstream and downstream links of the same physiological pathway at the same time to improve the efficiency of bone growth, or it may be that some components form a complex, while other components catalyze the formation of the complex and assist the complex in binding to the target.

[0010] In some embodiments, the mass fraction of the camel milk powder is 60-80%, the mass fraction of the complex minerals is 0.2-2%, the mass fraction of the complex vitamins is 1-5%, and the mass fraction of the complex active composition is 2-8%.

[0011] In some embodiments, the mass ratio of magnesium oxide, ferrous fumarate and zinc gluconate is 1-5:1-2:1-5.

[0012] In some embodiments, the mass ratio of vitamin B5, vitamin K2, and vitamin K3 is 1-8:1-5:1-5.

[0013] In some embodiments, the Pueraria lobata polypeptide, Gardenia jasminoides polypeptide, and Poria cocos polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural Pueraria lobata protein, natural Gardenia jasminoides protein, and natural Poria cocos protein, respectively, and the enzyme includes papain.

[0014] Since different enzymes have different cleavage sites on natural proteins, it is understandable that the small molecule peptides obtained by different enzymes have different sequences or spatial configurations, which will have a significant impact on the effect of formula camel milk powder. First, plant-derived proteases were selected in order to obtain small molecule peptides that are different from those obtained by human or animal digestion. The results showed that the effect of small molecule peptides hydrolyzed by plant-derived proteases was significantly better than directly using natural proteins. However, proteases from different plant sources theoretically have an impact on the auxiliary growth effect of the final formula camel milk powder. Further experiments selected papain, while bromelain and others had no obvious effect on auxiliary growth.

[0015] In some embodiments, the mass ratio of the Pueraria polypeptide, the Gardenia polypeptide, and the Poria polypeptide is 1-3:1-5:1-3.

[0016] In some embodiments, the compound active composition further comprises a Salvia miltiorrhiza extract.

[0017] Salvia miltiorrhiza extract is a mixture of active substances extracted from Salvia miltiorrhiza, including tanshinone, salvia miltiorrhiza phenol, etc. There is currently a mature extraction process. There is no significant difference in the Salvia miltiorrhiza extract whether it is directly purchased or obtained according to the preparation method in the experiment of the present invention.

[0018] Growth retardation can be divided into pathological and non-pathological. Non-pathological growth retardation is caused by genes, acquired environment, etc. Non-pathological growth retardation can be improved to a certain extent by the above-mentioned formula camel milk powder that does not contain Salvia miltiorrhiza extract, while pathological growth retardation is mainly caused by insufficient hormone levels, diseases, etc. When exploring the effect of formula camel milk powder on pathological growth retardation, the present invention accidentally discovered that the formula camel milk powder containing Salvia miltiorrhiza extract in previous experiments had a significant effect on promoting bone accumulation in osteoporosis model rats, suggesting that Salvia miltiorrhiza extract can further assist formula camel milk powder in promoting bone growth and bone health in individuals with pathological growth retardation. The inventor speculates that the active ingredients in Salvia miltiorrhiza extract (salvia miltiorrhiza phenol, etc.) may be able to catalyze the mutual binding and binding of other active substances in formula camel milk powder with the target. Formula camel milk powder containing Salvia miltiorrhiza extract can be used as a special food to assist adolescents with pathological growth retardation to grow taller.

[0019] In some embodiments, the Salvia miltiorrhiza extract accounts for 5-20% by weight of the composite active composition.

[0020] On the other hand, the present invention provides a composition for preparing an agent for promoting individual linear growth and bone mass accumulation, the composition comprising camel milk powder, compound minerals, compound vitamins and a compound active composition; the compound minerals comprising magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins comprising vitamin B5, vitamin K2 and vitamin K3; the compound active composition comprising kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein, respectively.

[0021] On the other hand, the present invention provides a use of a salvia miltiorrhiza extract for preparing an agent for improving the efficacy of formula camel milk powder in assisting an individual to grow taller, the formula camel milk powder comprising camel milk powder, compound minerals, compound vitamins and a compound active composition; the compound minerals comprising magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins comprising vitamin B5, vitamin K2 and vitamin K3; the compound active composition comprising kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein, respectively; the individual comprises an individual with pathological growth retardation.

[0022] The definition of pathological growth retardation is growth retardation caused by organic lesions, such as growth retardation caused by secondary (hypothalamic-pituitary and above) hypogonadism or hypothyroidism. For patients with pathological growth retardation, the usual treatment is to treat organic lesions and use exogenous growth hormone. The present invention provides a new way, that is, taking the formula camel milk powder of the present invention while treating organic lesions can also help adolescents grow taller.

[0023] The "individual" in the present invention refers to the concept of individuals in the popular understanding, including humans and mammals.

[0024] In summary, the present invention includes at least one of the following beneficial technical effects:

[0025] 1. The present invention discloses a formula camel milk powder with the function of assisting teenagers to grow taller, comprising camel milk powder, compound minerals, compound vitamins and compound active compositions, wherein all ingredients are from camel milk, trace elements, vitamins and natural plants that are both medicinal and edible and harmless to the human body, and are highly safe; the ingredients have a synergistic effect, and supplement and help children and teenagers improve bone health, increase linear growth and promote growth and development;

[0026] 2. For pathological growth retardation, the present invention found that formula camel milk powder containing Salvia miltiorrhiza extract has a significant effect on promoting bone accumulation in osteoporosis model rats, suggesting that Salvia miltiorrhiza extract can further assist formula camel milk powder in promoting bone growth and bone health in individuals with pathological growth retardation. For individuals with pathological growth retardation, the present invention provides a new approach, that is, taking the formula camel milk powder of the present invention while treating organic lesions can also play a role in assisting growth. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with specific examples, which are only used to explain the present invention and are not used to limit the scope of the present invention. The materials, reagents, etc. used in the following examples are reagents and materials available from commercial sources unless otherwise specified.

[0028] Example 1: Preparation of camel milk powder formula with the function of helping adolescents grow taller

[0029] Preparation of camel milk powder: Take fresh camel milk transported from the pasture, filter it using a double filter, and remove most of the impurities and microorganisms in the fresh camel milk through filtration and adsorption; then use a milk purifier to purify and sterilize it to further remove mammary gland cells and certain microorganisms in the fresh camel milk; then send it to a spray drying equipment for spray drying to obtain camel milk powder.

[0030] Preparation of compound minerals: Commercially available ferrous fumarate, zinc gluconate, and magnesium citrate are mixed in a mass ratio of 1:1:1.

[0031] Preparation of compound vitamins: Commercially available vitamin B5, vitamin K2, and vitamin K3 are mixed in a mass ratio of 3:3:1.

[0032] Preparation of the composite active composition: commercially available kudzu root, gardenia, and poria are taken, placed in containers respectively, petroleum ether is added and placed on a shaker for 24 hours, impurities are removed, and petroleum ether is removed by low-temperature reduced pressure distillation. The kudzu root, gardenia, and poria after impurities removal are placed in a 50°C oven for drying, taken out and cooled to room temperature, papain (Shanghai MacLean Company) is added and subjected to ultrasonic treatment, taken out after 3 hours and centrifuged, and freeze-dried precipitation is used to obtain the corresponding kudzu root polypeptide, gardenia polypeptide, and poria polypeptide; the above-mentioned kudzu root polypeptide, gardenia polypeptide, and poria polypeptide are mixed in a mass ratio of 1:1:1.

[0033] The raw material composition and ratio of the camel milk powder formula with the function of assisting teenagers to grow taller in this embodiment are shown in Table 1, wherein the raw materials are all prepared according to the above method or purchased from the market.

[0034] Table 1: Raw material composition of camel milk powder formula with the function of helping adolescents grow taller

[0035] serial number Raw material name Mass ratio (%) 1 Camel milk powder 75.0 2 lactose 5.0 3 Fructo-oligosaccharides 2.0 4 Inulin 1.0 5 Whey Protein Isolate Powder 10.0 6 Complex minerals 1.0 7 Vitamin complex 2.0 8 Compound active ingredients 5.0

[0036] The mixture prepared according to Table 1 is sent into a fluidized bed for thorough mixing and sifting to obtain a camel milk powder formula having the function of assisting adolescents to grow taller.

[0037] After further proportion tests, the preferred composition is as follows: the mass fraction of camel milk powder is 60-80%, the mass fraction of the complex minerals is 0.2-2%, the mass fraction of the complex vitamins is 1-5%, the mass fraction of the complex active composition is 2-8%, and the remaining components are adaptively filled with auxiliary materials such as lactose and oligofructose. The preferred meaning is that it can produce extremely significant synergistic effects.

[0038] Example 2: Comparison of the efficacy of camel milk powder with different formulas

[0039] The core of the present invention is to find a camel milk powder formula with synergistic effects. There is a synergistic effect between the ingredients in camel milk and the compound minerals, compound vitamins, and compound active compositions. The present invention has been experimentally demonstrated in the early stage, as follows:

[0040] 160 3-week-old C57BL / 6J mice (80 females and 80 males) were selected. After 1 week of adaptive feeding, they reached 4 weeks of age, corresponding to the puberty and growth period of mice. They were divided into 8 groups according to the principle of even distribution of males and females and random distribution, with 20 mice in each group, and given different feeding treatments. The specific grouping of the 8 groups is as follows:

[0041] Negative control group: fed with basic feed;

[0042] Positive control group: fed with growth and development feed;

[0043] Experimental group 1: fed with basic feed + daily gavage of camel milk powder 10 g / kg (bw), the camel milk powder was the camel milk powder prepared in Example 1;

[0044] Experimental group 2: fed with basic feed + daily gavage of camel milk powder 10 g / kg (bw), the camel milk powder was prepared according to the method of Example 1, but without adding compound minerals, compound vitamins, and compound active composition;

[0045] Experimental group 3: fed with basic feed + daily gavage of camel milk powder 10 g / kg (bw), the camel milk powder was prepared according to the method of Example 1, but without adding compound minerals;

[0046] Experimental group 4: fed with basic feed + daily gavage of camel milk powder 10 g / kg (bw), the camel milk powder was prepared according to the method of Example 1, but without adding compound vitamins;

[0047] Experimental group 5: fed with basic feed + daily gavage of camel milk powder 10 g / kg (bw), the camel milk powder was prepared according to the method of Example 1, but without adding the compound active composition;

[0048] Experimental group 6: fed with basic feed + daily intragastric administration of milk powder 10 g / kg (bw), the milk powder was prepared according to the method of Example 1, but the camel milk powder in the raw material was replaced by cow milk powder, and the preparation method of the cow milk powder was to replace the fresh camel milk in the preparation method of the camel milk powder with fresh cow milk;

[0049] Since the growth period and sexual maturity period of mice are 4-6 weeks and 6-8 weeks of age, respectively, the experiment was carried out for 4 weeks. During the experiment, the height of each group of mice was measured every 2 weeks. The bone density of the mice was tested 4 weeks after the start of the experiment. Specifically, the lumbar bone density of the mice was detected using a dual-energy X-ray absorptiometry instrument. The mean height of each group of mice at the beginning of the experiment, 2 weeks after the start of the experiment, and 4 weeks after the start of the experiment are shown in Table 2; the mean bone density of each group of mice 4 weeks after the start of the experiment is shown in Table 3.

[0050] Table 2: Mean height of mice in each group (n=20)

[0051]

[0052] The negative control group reflects the height development of mice under normal feeding conditions, and the positive control group reflects that the height development of mice is promoted when fed with a known growth and development feed; the mice in experimental group 1 are gavaged daily with the formula camel milk powder with the function of assisting adolescents to grow taller prepared in Example 1 on the basis of the basic feed. The mean height of mice at 6 weeks of age is higher than that of other groups, indicating that the formula camel milk powder in Example 1 has the function of assisting the growth of mice. Here, the height mainly reflects the linear growth of mice. The mean height of mice in other experimental groups is not significantly different from that of the negative control group.

[0053] Table 3: Mean bone density of mice in each group (n=20)

[0054]

[0055]

[0056] Bone density is a key indicator of bone strength. Higher bone density is usually associated with stronger bone structure, while lower bone density may indicate osteoporosis or skeletal dysplasia. In mice in the growth and development stage, higher bone density indicates more complete bone development, while lower bone density indicates slow bone development. The above bone density test results show that, except for experimental group 1, there is no significant difference in bone density between the other groups of mice at different time points, while the bone density of mice in experimental group 1 is much higher than that of other groups at 8 weeks of age. Combined with the height development, it can be seen that the formula camel milk powder in Example 1 mainly promotes bone accumulation in mice at 6-8 weeks of age.

[0057] Therefore, the camel milk powder formula in Example 1 has the effect of promoting linear growth and bone mass accumulation for mice in the growth and development stage. It mainly plays a role in promoting linear growth during the growth period and mainly plays a role in promoting bone mass accumulation during the sexual maturity period. The physiological mechanism and process of bone growth in adolescents are similar to those of mice. It can be understood that the camel milk powder formula in Example 1 can not only promote the height growth of adolescents, but also improve the bone health of adolescents when they grow taller.

[0058] Combined with the differences between different experimental groups, it can be inferred that the formula camel milk powder in Example 1 requires the simultaneous presence of camel milk, complex minerals, complex vitamins, and complex active compositions to promote the growth of mice, suggesting that there is a synergistic effect between the components in camel milk and the complex minerals, complex vitamins, and complex active compositions.

[0059] Example 3: Composition test of camel milk formula

[0060] In the previous research, a large number of formula composition tests were conducted, part of which is presented in the test of Example 2. Therefore, the inventors found that the ingredients in camel milk and external substances can play a synergistic effect in assisting adolescents to grow taller. Therefore, further experiments were conducted on adding, deleting and replacing the substances in the formula, hoping to find a core composition that produces a synergistic effect. This example lists another part of the formula composition test related to the specific substances in the formula.

[0061] 1. Composition test of compound minerals

[0062] The mineral elements that promote the growth of teenagers mainly include calcium, phosphorus, magnesium, zinc and iron. These minerals play a vital role in the growth and development of teenagers. Calcium is the basic element for bone growth and development. Adequate calcium intake is conducive to promoting the strength and growth of bones; phosphorus is an important component of bones, and it works together with calcium to promote the elongation and strengthening of bones; magnesium is conducive to maintaining bone health and neuromuscular function, and promotes normal bone development; zinc participates in enzyme synthesis and energy metabolism, and has an important influence on growth and development; iron is an important component of hemoglobin, participates in the transportation of oxygen, and plays an important role in growth and development.

[0063] It is generally believed that the chemical forms and composition ratios of various mineral elements are correlated with the function of promoting the growth of adolescents. Therefore, this embodiment configures salts of various chemical forms and compound minerals in different corresponding proportions, which are combined with other raw materials in Example 1 to form camel milk powder. The experiment is carried out according to the method of Example 2, and the body length of C57BL / 6J mice at 4-8 weeks of age that are gavaged with camel milk powder with different compound mineral compositions is mainly tested. According to the results of Example 2, the body length at 6 weeks of age is mainly selected as the evaluation index. The specific compound mineral composition and test results in some representative experiments are shown in Table 4.

[0064] Table 4: Body length of mice fed with camel milk powder with different mineral components at 6 weeks of age (n=20)

[0065]

[0066]

[0067] According to the above test results, it can be seen that adding beneficial mineral elements does not necessarily promote the linear growth of mice. For example, it is generally believed that the calcium content in camel milk is lower than that in cow milk, goat milk, etc. However, simply adding calcium to camel milk powder, such as seaweed calcium or calcium hydrogen phosphate in the experiment, cannot promote linear growth. This is because the absorption and bioutilization of calcium is a complex mechanism, which must be coordinated with a variety of substances to promote the corresponding physiological processes, and it cannot be achieved by simple addition; in addition, the compound form of minerals has a significant effect on the effectiveness of formula camel milk powder. The inventor's current experiments can only support the composite minerals composed of ferrous fumarate, zinc gluconate, and magnesium citrate. The invention can promote the linear growth of mice. The principle may be that the three mineral molecules can combine with other active substances in formula camel milk powder through electronic coordination and the like. The formed complex may have the function of binding to specific receptors in the body, thereby activating the physiological pathway of linear growth. Finally, further proportion tests show that most mass ratios have the function of promoting the linear growth of mice. The inventor's current tests show that the mass ratio of ferrous fumarate, zinc gluconate and magnesium citrate in the range of (1-5):(1-2):(1-5) has a better effect. The ratio in Example 1 is the most preferred. Only two test results are cited as prompts in the above test.

[0068] 2. Composition test of compound vitamins

[0069] It is generally believed that vitamin D plays a key role in promoting height growth, and its mechanism is mainly to promote calcium absorption. However, the role or auxiliary role of other vitamins in linear growth is not understood. In preliminary experiments, the inventors speculated that several currently known vitamins may be able to promote the absorption and utilization of active ingredients in foods that are both medicinal and edible, stimulate the relevant pathways of bone growth in individuals, and thus promote height growth and bone health in adolescents.

[0070] Therefore, this embodiment configures a plurality of compound vitamins with different vitamin compositions, and the compound vitamins are combined with the other raw materials in Example 1 to form camel milk powder. The experiment is carried out according to the method of Example 2, and the body length of C57BL / 6J mice at 4-8 weeks of age that are gavaged with camel milk powder with different compound vitamin compositions is mainly tested. According to the results of Example 2, the body length at 6 weeks of age is mainly selected as the evaluation index. The specific compound vitamin composition and the test results in some representative experiments are shown in Table 5.

[0071] Table 5: Body length of mice fed with camel milk powder with different vitamin complex compositions at 6 weeks of age (n=20)

[0072]

[0073]

[0074] According to the above test results, it can be seen that adding vitamin D3, which is generally believed to promote growth, is not enough to promote the linear growth of mice. This is because camel milk has a complex composition of bioactive substances and needs to be coordinated with a variety of substances to activate linear growth-related pathways in order to achieve the function of promoting growth. The inventor's current experiments can only support that camel milk powder composed of vitamin B5, vitamin K2, and vitamin K3 has the effect of promoting the linear growth of mice. When any one of the vitamins is replaced by another vitamin of the same family, its effect of promoting the linear growth of mice disappears. It can be seen that the main reason affecting its role in promoting linear growth is the chemical structure of the molecule, as well as the interaction between the corresponding chemical structure and the components, minerals and active compositions in camel milk. Among them, vitamin B5 (Pantothenic acid) is converted into coenzyme A (CoA) or acyl carrier protein (ACP) in the body. CoA is a cofactor of more than 70 enzymes in the body and may be involved in the modification of peptides in the active composition. ACP may be involved in the transport of peptides in the active composition, etc. Similarly, vitamin K2 has a certain effect of promoting osteocalcin synthesis. This effect may depend on the interaction with the structural analog vitamin K3 and the above-mentioned vitamin B5, and may also be related to the combination of minerals and the active composition; finally, further ratio tests show that the mass ratio of vitamin B5, vitamin K2, and vitamin K3 is better in the range of (1-8): (1-5): (1-5), and the ratio in Example 1 is the most preferred. In the above test, only two test results are cited as prompts.

[0075] 3. Composition test of compound active composition

[0076] It is generally believed that hawthorn, lily, lotus seeds and other medicinal materials have the effect of assisting height growth, but in practice, a combination of multiple medicinal materials must be boiled and taken for a long time, and there is no quantitative experimental evidence for their specific effects. The present invention attempts to extract active substances from medicinal materials and combine them with camel milk and other formulas to promote height growth and bone health in adolescents.

[0077] For the purpose of assisting absorption, the present invention enzymatically hydrolyzes the natural protein in the medicinal material to obtain small molecule peptides, which have higher absorption rate and bioavailability. To test the effect, enzymatically hydrolyzed and unenzymatically hydrolyzed composite active compositions are respectively prepared. The unenzymatically hydrolyzed composite active composition is prepared according to the method of Example 1, except that the natural protein is directly extracted without enzymatic hydrolysis. The test is carried out according to the method of Example 2, and the body length of C57BL / 6J mice at 4-8 weeks of age that are gavaged with camel milk powder composed of different composite active compositions is mainly tested. According to the results of Example 2, the body length at 6 weeks of age is mainly selected as the evaluation index. The test results are shown in Table 6.

[0078] Table 6: Body length of mice fed with camel milk powder of different compound active compositions at 6 weeks of age (n=20)

[0079] Grouping Height(mm) Enzymatic hydrolysis (Example 1) 104.5 No enzymatic hydrolysis 96.4

[0080] Therefore, it is necessary to enzymatically hydrolyze Pueraria, Gardenia and Poria, and the type of enzyme used for enzymatic hydrolysis determines the enzymatic site. Therefore, different proteases can obtain different polypeptides. In theory, different polypeptides have different effects. After testing, papain is preferably used for enzymatic hydrolysis.

[0081] Further attempts were made to construct a composite active composition using small molecule peptides of different medicinal materials after enzymatic hydrolysis. The composition was prepared according to the method of Example 1 and tested according to the method of Example 2. The body length of C57BL / 6J mice at 4-8 weeks of age that were gavaged with camel milk powder composed of different composite active compositions was mainly tested. According to the results of Example 2, the body length at 6 weeks of age was mainly selected as the evaluation index. The test results are shown in Table 7.

[0082] Table 7: Body length of mice fed with camel milk powder of different compound active compositions at 6 weeks of age (n=20)

[0083]

[0084]

[0085] According to the above test results, it can be seen that the use of medicinal materials that are generally believed to promote growth is not enough to assist the linear growth of mice. The polypeptides prepared from hawthorn, lily and lotus seeds do not assist the linear growth of mice in formula camel milk powder. The current experiments of the present invention only support that the formula camel milk powder composed of kudzu root polypeptide, gardenia polypeptide and poria polypeptide has the effect of promoting the linear growth of mice by oral administration. The reason may be that the above polypeptides have special sequences and molecular configurations, and have a good molecular docking mode with the active substances, compound minerals and compound vitamins in camel milk powder, and can produce coordination and other binding tendencies. The related polypeptides have a binding tendency with bone growth-related targets, etc. Since the kudzu root polypeptide, gardenia polypeptide and poria polypeptide of the present invention are all enzymatically hydrolyzed mixtures, the specific mechanism is relatively complex and needs further study. Therefore, other possible mechanisms are not excluded here. Finally, further ratio tests show that the mass ratio of kudzu root polypeptide, gardenia polypeptide and poria polypeptide is better in the range of (1-3): (1-5): (1-3), and the ratio in Example 1 is most preferred. In the above test, only two test results are cited as prompts.

[0086] Example 4: Bone mass accumulation test in osteoporosis model rats

[0087] In the above experiments, C57BL / 6J mice in the growth and development period were used as test subjects, which verified that the camel milk formula of the present invention has the effect of assisting the growth of individuals with normal growth and development. In order to further study the efficacy of the camel milk formula of the present invention, this example will use osteoporosis model rats as test subjects. Osteoporosis model rats lack sex hormones and their bone density slowly decreases after ovariectomy to simulate the pathological growth retardation of adolescents. The osteoporosis model rats were gavaged with camel milk formula to study the mechanism and effect of camel milk formula on bone accumulation in adolescents with pathological growth retardation.

[0088] Construction of osteoporosis model rats: Healthy 3-month-old SD rats were taken and subjected to ovariectomy after adaptive feeding for 1 week: after anesthesia, the ventral skin was prepared, depilated, and disinfected, and a sterile surgical scissors was used to cut an incision of about 2 cm in length in the midline of the abdomen to separate and expose the ovaries, and then the bilateral ovaries were removed after ligation with sutures. After the operation, the uterus was returned to the abdominal cavity, the muscles and skin were sutured layer by layer, and the wound was disinfected with iodine and placed on a heating pad for normal feeding after waking up. The blank control group underwent a sham operation: only anesthesia was used to make an incision, the ovaries were exposed but not ligated and removed, and returned to the abdominal cavity to suture the wound, and the rats were raised normally after waking up.

[0089] One week after surgery, 40 osteoporosis model rats were randomly selected and divided into two groups, 20 in each group. The experimental group was gavaged with 10 g / kg (bw) of camel milk powder daily, and the camel milk powder was prepared according to the method of Example 1; the positive control group was gavaged with an equal amount of normal saline daily; and 20 sham-operated rats were randomly selected to form a blank control group, which were gavaged with an equal amount of normal saline daily; the experiment was carried out for a total of 12 weeks, and after 12 weeks, the bone density of the lumbar vertebrae of the three groups of rats was detected using dual-energy X-ray absorptiometry, and the results are shown in Table 8.

[0090] Table 8: Mean bone density of rats in each group (n=20)

[0091] Grouping <![CDATA[Bone Mineral Density (BMD g / cm 2 )]]> Blank control group 0.241 Positive control group 0.206 Experimental Group 0.220

[0092] It can be seen that compared with the positive control group, the bone density of the rats in the experimental group was improved to a certain extent by gavage of the formula camel milk powder of the present invention. There may be two reasons for this. One is that in the absence of sex hormones, the bone growth-related pathways are activated, which slowly promotes bone growth. The other is that the degradation of bone tissue is inhibited. It is understandable that the formula camel milk powder of the present invention can also help adolescents with pathological growth retardation to grow taller and promote bone health.

[0093] In order to further improve the improvement effect of the camel milk powder of the present invention on pathological growth retardation, after a large number of formula experiments, the present invention found that the additional addition of Salvia miltiorrhiza extract to the composite active composition can further improve the bone density of osteoporosis model rats. Experimental group 2 was set up, and 10 g / kg (bw) of camel milk powder was gavaged daily. The camel milk powder was prepared according to the method of Example 1, except that the composite active composition therein was Pueraria polypeptide, Gardenia polypeptide, Poria polypeptide, and Salvia miltiorrhiza extract (mass ratio 1:1:1:0.5). The preparation method of Salvia miltiorrhiza extract was as follows: select high-quality Salvia miltiorrhiza raw materials, thoroughly wash them, dry them in a well-ventilated place with moderate temperature, grind them with a grinder to make them into particles of suitable size for extraction, soak them in 75% ethanol aqueous solution for 6 hours, then heat and stir for 2 hours, filter, and distill under reduced pressure; according to the above experimental method of this embodiment, the experiment was carried out, and the bone density of the rat lumbar vertebrae was 0.232 after 12 weeks, which was close to the level of the blank control.

[0094] Further proportion experiments showed that only a small amount of Salvia miltiorrhiza extract is needed to significantly improve the bone density of osteoporosis model rats. The Salvia miltiorrhiza extract only accounts for 5-20% of the weight of the composite active composition. Therefore, the inventors speculate that the active ingredients (salvia miltiorrhiza phenol, etc.) in the Salvia miltiorrhiza extract may be able to catalyze the mutual binding and binding of other active substances in the formula camel milk powder with the target. The formula camel milk powder containing Salvia miltiorrhiza extract can be used as a special food to assist adolescents with pathological growth retardation to grow taller.

[0095] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto, and any equivalent modifications or substitutions that can be easily thought of by any technician familiar with the technical field within the technical scope disclosed by the present invention should be included in the protection scope of the present invention.

Claims

1. A camel milk powder formula with the function of helping teenagers grow taller, characterized in that: The invention comprises the following raw materials: camel milk powder, compound minerals, compound vitamins and compound active compositions; the compound minerals comprise magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins comprise vitamin B5, vitamin K2 and vitamin K3; the compound active compositions comprise kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein respectively.

2. The camel milk powder formula having the function of assisting teenagers to grow taller as claimed in claim 1, characterized in that: The mass fraction of the camel milk powder is 60-80%, the mass fraction of the complex minerals is 0.2-2%, the mass fraction of the complex vitamins is 1-5%, and the mass fraction of the complex active composition is 2-8%.

3. The camel milk powder formula having the function of assisting teenagers to grow taller as claimed in claim 1 or 2, characterized in that: The mass ratio of the magnesium oxide, ferrous fumarate and zinc gluconate is 1-5:1-2:1-5.

4. The camel milk powder formula having the function of assisting teenagers to grow taller as claimed in claim 1 or 2, characterized in that: The mass ratio of vitamin B5, vitamin K2 and vitamin K3 is 1-8:1-5:1-5.

5. The camel milk powder formula having the function of assisting teenagers to grow taller as claimed in claim 1 or 2, characterized in that: The kudzu root polypeptide, gardenia polypeptide and tuckahoe polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural tuckahoe protein, respectively, and the enzyme includes papain.

6. The camel milk powder formula with the function of helping teenagers grow taller as claimed in claim 5, characterized in that: The mass ratio of the Pueraria polypeptide, the Gardenia polypeptide and the Poria polypeptide is 1-3:1-5:1-3.

7. The camel milk powder formula having the function of assisting teenagers to grow taller as claimed in claim 1 or 2, characterized in that: The compound active composition also includes a salvia miltiorrhiza extract.

8. The camel milk powder formula with the function of helping teenagers grow taller as claimed in claim 7, characterized in that: The salvia miltiorrhiza extract accounts for 5-20% by weight of the composite active composition.

9. A composition for use in preparing an agent for promoting linear growth and bone mass accumulation in an individual, characterized in that: The composition includes camel milk powder, compound minerals, compound vitamins and a compound active composition; the compound minerals include magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins include vitamin B5, vitamin K2 and vitamin K3; the compound active composition includes kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein respectively.

10. A use of a salvia miltiorrhiza extract for preparing a reagent for improving the efficacy of formula camel milk powder in assisting an individual to grow taller, characterized in that: The formula camel milk powder includes camel milk powder, compound minerals, compound vitamins and compound active compositions; the compound minerals include magnesium oxide, ferrous fumarate and zinc gluconate; the compound vitamins include vitamin B5, vitamin K2 and vitamin K3; the compound active compositions include kudzu root polypeptide, gardenia polypeptide and poria polypeptide; the kudzu root polypeptide, gardenia polypeptide and poria polypeptide are small molecule peptides obtained by enzymatic hydrolysis of natural kudzu root protein, natural gardenia protein and natural poria protein respectively; the individuals include individuals with pathological growth retardation.

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