Weight-losing composition as well as preparation method and application thereof

Abstract

The invention discloses a weight-losing composition as well as a preparation method and application thereof, and belongs to the field of foods. The weight-losing composition provided by the invention is prepared by taking stachys sieboldii powder, white radish powder, fucoidin, aloe extract powder and broccoli seed water extract as raw materials, fermenting the raw materials through a specific strain, and then filtering, sterilizing and freeze-drying a fermentation product. A multi-strain mixed fermentation process is adopted, and the weight losing effect of the composition can be effectively improved through the synergistic effect of different strains.

Description

Technical Field

[0001] The invention relates to the field of food additives, and in particular to a weight loss composition and a preparation method and application thereof. Background Art

[0002] Obesity has become a global public health problem. It not only affects people's appearance and ability to move, but also easily induces or aggravates a variety of chronic diseases, such as hypertension, hyperlipidemia, heart disease, diabetes, fatty liver, endocrine disorders, etc., which seriously endanger people's health. Obesity is weight gain caused by an increase in the volume of body fat or the number of fat cells, or an abnormal increase in the percentage of body fat to body weight, which manifests as excessive fat deposition throughout the body or in certain parts of the human body. In addition, due to the increased weight burden, obese patients often feel weak, tired, short of breath, difficulty in movement, joint and lower back pain, lower limb edema, etc., which brings inconvenience to daily life. At the same time, due to their ugly body shape, obese patients are prone to psychological disorders such as inferiority, depression, and anxiety.

[0003] Therefore, it is extremely important to prevent or treat the occurrence of obesity. At present, the main means of weight loss include exercise, diet control, drug therapy, surgical fat reduction, etc., but these methods have certain limitations. For example, exercise and dieting require a lot of time and self-control; drug therapy may have side effects, such as gastrointestinal discomfort, anorexia, rebound, etc.; surgical fat reduction and dieting may cause physical damage and skin sagging. Therefore, the development of a safe and effective weight loss composition can help people achieve the goal of healthy weight loss without affecting their normal life, which has important practical significance and broad market prospects. Summary of the invention

[0004] The purpose of the present invention is to provide a weight loss composition and its preparation process and application, wherein the weight loss composition is prepared by fermenting, filtering, sterilizing and freeze-drying with specific strains of manna seed powder, white radish powder, fucoidan, aloe extract powder and broccoli seed water extract as raw materials. The present invention adopts a multi-strain mixed fermentation process, and the weight loss effect of the extract can be effectively improved through the synergistic effect between different strains.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a weight loss composition, characterized in that it comprises the following raw materials in parts by weight:

[0007] Manna seed powder: 20-30 portions;

[0008] White radish powder: 10-15 portions;

[0009] Fucoidan: 10-12 parts;

[0010] Aloe vera extract powder: 8-10 parts;

[0011] Broccoli seed water extract: 6-8 servings.

[0012] Preferably, the weight loss composition is characterized by comprising the following raw materials in parts by weight:

[0013] Manna seed powder: 25-30 portions;

[0014] White radish powder: 10-13 portions;

[0015] Fucoidan: 10-11 parts;

[0016] Aloe vera extract powder: 9-10 parts;

[0017] Broccoli seed water extract: 6-7 servings.

[0018] Most preferably, the weight loss composition is characterized by comprising the following raw materials in parts by weight:

[0019] Manna seed powder: 25 parts;

[0020] White radish powder: 13 portions;

[0021] Fucoidan: 11 parts;

[0022] Aloe vera extract powder: 9 parts;

[0023] Broccoli seed aqueous extract: 7 parts.

[0024] In a second aspect, the present invention provides a method for preparing the weight loss composition, comprising the following preparation steps:

[0025] S1: Weigh each raw material according to the formula amount, and mix each raw material with sterile water to obtain a mixed solution, wherein the mass ratio of each raw material to sterile water is 1:2-4;

[0026] S2: inoculating Lactobacillus gasseri and Lactococcus lactis into the mixed solution obtained in S1 and then performing anaerobically fermentation for 8-10 hours, wherein the fermentation temperature is 30-32°C;

[0027] S3: After the fermentation in S2 is completed, sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 6-8 hours, wherein the fermentation temperature is 30-32°C and the sterile air introduction flow rate is 10-15L / min;

[0028] S4: after the fermentation in S3 is completed, sterilize and filter to obtain a fermentation filtrate, and concentrate and freeze-dry the fermentation filtrate to obtain a weight loss composition;

[0029] Wherein, the Lactobacillus gasseri in step S2 is purchased from China General Microorganism Culture Collection Center, numbered CGMCC NO.1.3224; the Lactococcus lactis is purchased from China General Microorganism Culture Collection Center, numbered CGMCC NO.1.62; the Acetobacter aceti in step S3 is purchased from China General Microorganism Culture Collection Center, numbered CGMCC NO.1.1809; the mass ratio of the Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:0.5-1.5:1-1.5; the total addition amount of the Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti accounts for 1-3wt% of the mixed solution.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides a weight loss composition and a preparation method and application thereof, wherein the weight loss composition uses manna seed powder, white radish powder, fucoidan, aloe extract powder, and broccoli seed water extract as raw materials, the above raw materials are rich in dietary fiber and help to enhance satiety, wherein the mustard oil and sulfide contained in the white radish, the polysaccharides and fucoxanthin contained in the fucoidan, and the glucoraphanin rich in the broccoli seed water extract have a significant effect of reducing the absorption and accumulation of fat, and in addition, the aloe-emodin and aloin in the aloe have the effects of promoting intestinal peristalsis and reducing the secondary absorption of fat. Based on the above raw material characteristics, the present invention innovatively first uses Lactobacillus gasseri and Lactococcus lactis to perform anaerobic fermentation on each raw material, and then uses Acetobacter aceti, Lactobacillus gasseri and Lactococcus lactis to perform aerobic fermentation on each raw material, and then filters and freeze-dries the fermentation product to prepare a weight loss composition, wherein mannoses powder, white radish powder, fucoidan, aloe extract powder, and broccoli seed water extract can produce lactic acid, short-chain fatty acids, vitamins, amino acids, organic acids, etc. after anaerobic fermentation by Lactobacillus gasseri and Lactococcus lactis, wherein lactic acid and organic acids can be used as raw materials for Acetobacter aceti to be reused , fermentation produces organic acids and enzymes such as ethyl acetate, acetoin, ligustrazine, and gluconic acid, and after the introduction of oxygen, Lactobacillus gasseri and Lactococcus lactis can produce a variety of organic acids and enzymes again through aerobic fermentation; the raw materials are fermented twice to obtain fermentation products rich in acetic acid, organic acids, enzymes and amino acid compounds, among which acetic acid can enhance satiety, delay the rise in blood sugar after a meal, and reduce fat synthesis caused by increased blood sugar; organic acids can promote metabolism; enzymes and amino acids can help reduce fat accumulation by regulating blood sugar and improving insulin sensitivity. DETAILED DESCRIPTION

[0031] The embodiments described below are part of the embodiments of the present invention, rather than all of the embodiments. The embodiments of the present invention are used to illustrate the present invention, rather than to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0032] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturers. The materials and reagents used can be obtained from commercial sources unless otherwise specified.

[0033] Some raw materials and their sources are as follows:

[0034] Manluzi: purchased from the farmers' market;

[0035] White radish: purchased from the farmers market;

[0036] Fucoidan: purchased from Xi'an Zebang Biotechnology Co., Ltd.;

[0037] Aloe vera extract powder: purchased from Xi'an Ruiying Biotechnology Co., Ltd.;

[0038] Broccoli seed water extract: purchased from Xi'an Haoyu Kangze Biotechnology Co., Ltd.;

[0039] Lactobacillus gasseri: purchased from China General Microbiological Culture Collection Center, number CGMCCNO.1.3224;

[0040] Lactococcus lactis: purchased from China General Microbiological Culture Collection Center, number CGMCC NO.1.62;

[0041] Acetobacter aceti: ​​purchased from China General Microbiological Culture Collection Center, number CGMCCNO.1.1809;

[0042] Lactobacillus plantarum: purchased from China General Microbiological Culture Collection Center, CGMCC NO.1.16089;

[0043] Pediococcus acidilactici number: purchased from China General Microbiological Culture Collection Center, CGMCCNO.1.12332.

[0044] The weight percentages of the various raw materials in the weight loss composition are as follows:

[0045] Weight Loss Composition 1:

[0046] Manna seed powder: 25 parts; white radish powder: 13 parts; fucoidan: 11 parts; aloe extract powder: 9 parts; broccoli seed water extract: 7 parts.

[0047] Weight Loss Composition 2:

[0048] Manna seed powder: 30 parts; white radish powder: 10 parts; fucoidan: 12 parts; aloe extract powder: 8 parts; broccoli seed water extract: 8 parts.

[0049] Weight Loss Composition 3:

[0050] Manna seed powder: 20 parts; white radish powder: 15 parts; fucoidan: 10 parts; aloe extract powder: 10 parts; broccoli seed water extract: 6 parts.

[0051] Weight loss composition ①:

[0052] The difference from the weight loss composition 1 is that the manna seed powder is missing, and the missing mass is distributed to the remaining components according to the mass ratio in the weight loss composition 1.

[0053] Weight loss composition ②

[0054] The difference from the weight loss composition 1 is that fucoidan is missing, and the missing mass is distributed to the remaining components according to the mass ratio in the weight loss composition 1.

[0055] Weight loss composition ③

[0056] The difference from the weight loss composition 1 is that the broccoli seed water extract is missing, and the missing mass is distributed to the remaining components according to the mass ratio in the weight loss composition 1.

[0057] The preparation methods of the above-mentioned weight loss composition 1 and weight loss compositions ①-③ are as follows:

[0058] S1: Weigh each raw material according to the formula amount, mix each raw material with sterile water to obtain a mixed solution, wherein the mass ratio of each raw material to sterile water is 1:3;

[0059] S2: Inoculate Lactobacillus gasseri and Lactococcus lactis into the mixed solution obtained in S1 and then perform anaerobically fermentation for 9 hours, wherein the fermentation temperature is 31°C;

[0060] S3: After the fermentation in S2 is completed, sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 7 hours, wherein the fermentation temperature is 31°C and the sterile air introduction flow rate is 13L / min;

[0061] S4: after the fermentation in S3 is completed, the fermentation product is sterilized, filtered to obtain a fermentation filtrate, and the fermentation filtrate is concentrated and freeze-dried to obtain a weight loss composition;

[0062] The mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:1:1.3; the total addition amount of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti accounts for 2wt% of the mixed solution.

[0063] The preparation method of the weight loss composition 2 is as follows:

[0064] S1: Weigh each raw material according to the formula amount, mix each raw material with sterile water to obtain a mixed solution, wherein the mass ratio of each raw material to sterile water is 1:2;

[0065] S2: Inoculate Lactobacillus gasseri and Lactococcus lactis into the mixed solution obtained in S1 and then perform anaerobically fermentation for 8 hours, wherein the fermentation temperature is 30°C;

[0066] S3: After the fermentation in S2 is completed, sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 6 hours, wherein the fermentation temperature is 30°C and the sterile air introduction flow rate is 10L / min;

[0067] S4: after the fermentation in S3 is completed, the fermentation product is sterilized, filtered to obtain a fermentation filtrate, and the fermentation filtrate is concentrated and freeze-dried to obtain a weight loss composition;

[0068] The mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:0.5:1.5; the total addition amount of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti accounts for 3wt% of the mixed solution.

[0069] The preparation method of the weight loss composition 3 is as follows:

[0070] S1: Weigh each raw material according to the formula amount, mix each raw material with sterile water to obtain a mixed solution, wherein the mass ratio of each raw material to sterile water is 1:4;

[0071] S2: Inoculate Lactobacillus gasseri and Lactococcus lactis into the mixed solution obtained in S1 and then perform anaerobically fermentation for 10 hours, wherein the fermentation temperature is 32°C;

[0072] S3: After the fermentation in S2 is completed, sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 8 hours, wherein the fermentation temperature is 32°C and the sterile air introduction flow rate is 15L / min;

[0073] S4: after the fermentation in S3 is completed, the fermentation product is sterilized, filtered to obtain a fermentation filtrate, and the fermentation filtrate is concentrated and freeze-dried to obtain a weight loss composition;

[0074] The mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:1.5:1; the total addition amount of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti accounts for 1wt% of the mixed solution.

[0075] Weight loss composition ④:

[0076] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that in step S3, after the fermentation of S2 is completed, sterilization is performed, and then sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 7 hours, wherein the fermentation temperature is 31° C., and the sterile air introduction flow rate is 13 L / min, and the remaining steps are the same as those of composition 1.

[0077] Weight loss composition ⑤:

[0078] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that Lactobacillus plantarum numbered CGMCC NO.1.16089 is used to replace Lactobacillus gasseri, and the remaining steps are the same as those of composition 1.

[0079] Weight loss composition ⑥:

[0080] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that lactococcus acidilactici number: CGMCC NO.1.12332 is used to replace Lactococcus lactis number: CGMCC NO.1.62, and the remaining steps are the same as those of composition 1.

[0081] Weight loss composition ⑦:

[0082] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that the mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:1:2.

[0083] Weight loss composition⑧:

[0084] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that the mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:1:0.5.

[0085] Weight loss composition ⑨:

[0086] The raw materials are the same as those of composition 1, but the preparation method is different from that of composition 1, the difference being that the mass ratio of Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:2:1.3.

[0087] Experiment 1: Verification of weight loss and lipid-lowering efficacy

[0088] Test samples: Compositions 1-3 and compositions ①-⑨, wherein compositions 1-3 and ①-⑨ are prepared into 10 g / L liquid using physiological saline.

[0089] Detection method: Kunming mice weighing 20±2g were selected. After 7 days of adaptive feeding, the experiment was started. The mice were evenly divided into 14 groups (blank control group, obesity model group, sample group), with 9 mice in each group. Among them, the blank control group was fed with ordinary feed, and the obesity model group and the model group were fed with high-fat feed. The feeding was continued for 30 days to establish a nutritional obesity model. After the modeling was successful, the sample group was gavaged with samples from the 31st day, with a gavage volume of 10mL / kg. The blank control group and the obesity model group were gavaged with an equal amount of normal saline, and each group was quantitatively fed from the 31st day. The blank group continued to be fed with ordinary feed, and the other groups were fed with high-fat feed; after 40 days of the experiment, the body weight was weighed 12 hours after the last gavage. After the end of the experiment, the mice were fasted for 12 hours, and blood was collected from the eye sockets. The triglyceride and cholesterol levels in the serum were determined using a kit. The data were recorded as the mean ± standard deviation, and the data were retained to two decimal places; where Δ body weight = (final body weight - initial body weight) / initial body weight * 100%.

[0090] Table 1 Verification of weight loss and lipid-lowering efficacy

[0091] Experimental Group ΔBW / % Triglyceride content (mmol / L) Cholesterol content (mmol / L) Blank control group 35.34±1.61# 1.49±0.08# 2.17±0.15# Obesity model group 46.07±1.95* 3.04±0.19* 3.88±0.14* Composition 1 37.21±0.52# 1.77±0.15# 2.49±0.09# Composition 2 38.32±0.17# 2.08±0.16# 2.70±0.23# Composition 3 37.92±1.12# 1.90±0.13# 2.60±0.10# Composition ① 41.13±1.17#a 2.72±0.09#a 3.46±0.16#a Composition ② 42.31±1.50#a 2.78±0.05#a 3.51±0.05#a Composition ③ 41.48±0.61#a 2.75±0.07#a 3.50±0.17#a Composition ④ 41.27±1.40#a 2.70±0.04#a 3.45±0.19#a Composition ⑤ 41.42±1.79#a 2.74±0.10#a 3.46±0.06#a Composition ⑥ 40.17±0.60#a 2.70±0.12#a 3.39±0.02#a Composition ⑦ 39.75±0.36#a 2.67±0.06#a 3.35±0.05#a Composition⑧ 39.94±0.56#a 2.69±0.23#a 3.08±0.09#a Composition ⑨ 40.55±0.47#a 2.57±0.25#a 3.26±0.04#a

[0092] Note: “#” indicates comparison with the obesity model group, p < 0.05; “*” indicates comparison with the blank control group, p < 0.05; “a” indicates comparison with composition 1, p < 0.05.

[0093] According to the results in Table 1, compared with the obesity model group, the compositions 1-3 have significant weight loss and lipid-lowering effects.

[0094] Comparison of the results of composition 1 and compositions ①-③ shows that the manna seed powder, white radish powder, fucoidan, aloe extract powder and broccoli seed water extract in the present invention have significant synergistic effects.

[0095] In composition 1, Lactobacillus gasseri and Lactococcus lactis are firstly used for anaerobic fermentation, and after the anaerobic fermentation is completed, sterile air is introduced, and then Acetobacter aceti is inoculated, and Lactobacillus gasseri, Lactococcus lactis, and Acetobacter aceti are used for aerobic fermentation. In composition ④, Lactobacillus gasseri and Lactococcus lactis are firstly used for anaerobic fermentation, and after the anaerobic fermentation is completed, sterilization is carried out, and then sterile air is introduced, Acetobacter aceti is inoculated, and Acetobacter aceti is used for aerobic fermentation. According to the comparison of the results of composition 1 and composition ④ in Table 1, it can be seen that the fermentation method used in composition 1 of the present invention can significantly improve the weight loss and lipid-lowering effect of the weight loss composition.

[0096] Comparison of the results of composition 1 and compositions ⑤-⑥ shows that the bacterial species described in the present invention have significant synergistic effects.

[0097] Comparison of the results of composition 1 and compositions ⑦-⑨ shows that Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti in the present invention have significant synergistic effects within the mass range defined in the present invention.

[0098] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

Claims

1. A weight loss composition, characterized in that: Contains the following raw materials by weight: Manna seed powder: 20-30 portions; White radish powder: 10-15 portions; Fucoidan: 10-12 parts; Aloe vera extract powder: 8-10 parts; Broccoli seed water extract: 6-8 parts; The preparation method of the weight loss composition is as follows: S1: Weigh each raw material according to the formula amount, and mix each raw material with sterile water to obtain a mixed solution, wherein the mass ratio of each raw material to sterile water is 1:2-4; S2: inoculating Lactobacillus gasseri and Lactococcus lactis into the mixed solution obtained in S1 and then performing anaerobically fermentation for 8-10 hours, wherein the fermentation temperature is 30-32°C; S3: After the fermentation in S2 is completed, sterile air is introduced into the fermentation liquid, and then Acetobacter aceti is inoculated and fermented for 6-8 hours, wherein the fermentation temperature is 30-32°C and the sterile air introduction flow rate is 10-15L / min; S4: after the fermentation in S3 is completed, sterilize and filter to obtain a fermentation filtrate, and concentrate and freeze-dry the fermentation filtrate to obtain a weight loss composition; Wherein, the Lactobacillus gasseri in step S2 is purchased from China General Microorganism Culture Collection Administration Center, numbered CGMCC NO.1.3224; the Lactococcus lactis is purchased from China General Microorganism Culture Collection Administration Center, numbered CGMCC NO.1.62; the Acetobacter aceti in step S3 is purchased from China General Microorganism Culture Collection Administration Center, numbered CGMCC NO.1.1809; the mass ratio of the Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti is 1:0.5-1.5:1-1.5; the total addition amount of the Lactobacillus gasseri, Lactococcus lactis and Acetobacter aceti accounts for 1-3wt% of the mixed solution.

2. The composition according to claim 1, characterized in that The preparation method of the mannose seed powder has the following steps: Wash and slice the manna seeds, dry them to constant weight, and then grind them into powder. After passing through a 80-120 mesh sieve, seal and store away from light.

3. The composition according to claim 1, characterized in that The preparation method of the white radish powder has the following steps: Wash and slice the white radish, dry it to constant weight, and then crush it. After passing through an 80-120 mesh sieve, seal it and store it away from light.

4. The composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 25-30 parts of mannose seed powder; White radish powder: 10-13 portions; Fucoidan: 10-11 parts; Aloe vera extract powder: 9-10 parts; Broccoli seed water extract: 6-7 servings.

5. The composition according to claim 1, characterized in that Contains the following raw materials by weight: 25 parts of mannose powder; White radish powder: 13 parts; Fucoidan: 11 parts; Aloe vera extract powder: 9 parts; Broccoli seed aqueous extract: 7 parts.

6. Use of the weight loss composition according to any one of claims 1 to 3 in the preparation of a weight loss product.