A blood sampling device for mouse retrobulbar vein

Abstract

The present application discloses a mouse retrobulbar vein blood sampling device, which relates to the technical field of blood sampling instruments, including a base plate, a movable plate, a support rod, an L-shaped bracket and a needle holder, wherein the base plate is provided with limb fixings for fixing the mouse's limbs; the movable plate is flipped and arranged on the base plate, and the movable plate is provided with a first fixing, a second fixing and a third fixing for fixing the mouse's left ear canal, right ear canal and incisors; the support rod is used to stand the movable plate vertically to the base plate; the L-shaped bracket is arranged on the side of the movable plate that is not connected to the base plate, and the L-shaped bracket slides along the movable plate; the needle holder slides along the L-shaped bracket, and a needle sleeve for needle insertion is provided on the needle holder, and the needle sleeve is at a 45-degree angle with the plane determined by the first, second and third fixings. The present application can accurately control the needle insertion angle, and conveniently complete the retrobulbar vein blood sampling of mice.

Description

Technical Field

[0001] The present application relates to the technical field of blood sampling instruments, and in particular to a retrobulbar vein blood sampling device for mice. Background Art

[0002] Blood sampling from the retrobulbar vein of mice is one of the commonly used sample collection methods in pharmacology or pharmacokinetic studies.

[0003] Currently, blood is collected from the retrobulbar vein of mice by anesthetizing the mice and then the experimenter holds a capillary glass tube and inserts the needle into the retrobulbar vein at a specified angle based on experience to complete the blood collection. However, needle insertion based on experience is not standardized and may result in residual glass fragments or errors in the needle insertion angle, which may easily cause infection or even blindness in the mice. The amount of blood collected cannot be controlled, leading to the risk of anemia or even death from hemorrhagic shock. Summary of the invention

[0004] The main purpose of the present application is to provide a mouse retrobulbar vein blood sampling device, aiming to solve the above-mentioned technical problems.

[0005] The technical solutions adopted in this application are as follows:

[0006] A mouse retrobulbar vein blood sampling device, comprising:

[0007] A bottom plate, wherein limb fixing parts for fixing the limbs of the mouse are arranged on the bottom plate;

[0008] A movable plate, the movable plate is flipped and arranged on the bottom plate, and the movable plate is provided with a first fixing member, a second fixing member and a third fixing member for fixing the left ear canal, the right ear canal and the incisor of the mouse;

[0009] A support rod, one end of which is hinged to the bottom plate and the other end of which is detachably connected to the movable plate, and the support rod can erect the movable plate perpendicular to the bottom plate;

[0010] An L-shaped bracket, the L-shaped bracket is arranged on a side of the movable plate that is not connected to the bottom plate, and the L-shaped bracket slides along the movable plate;

[0011] The needle rack slides along the L-shaped bracket, and a needle sleeve for inserting the needle is arranged on the needle rack, and the needle sleeve forms an angle of 45 degrees with the plane defined by the first fixing member, the second fixing member and the third fixing member.

[0012] Optionally, a hinge seat is provided on the plate surface of the movable plate for connecting the support rod, the hinge seat is provided with a connecting pin hole, a spring pin is provided at one end of the support rod connected to the movable plate, the support rod is placed in the hinge seat and the spring pin is embedded in the connecting pin hole.

[0013] Optionally, the support rod includes an outer rod and an inner rod, the outer rod and the inner rod are slidably connected, the outer sleeve is movably connected to the base plate, the inner rod is connected to the movable plate, and a placement groove for placing the support rod is provided on the base plate.

[0014] Optionally, an adjusting member for adjusting the sliding movement of the L-shaped bracket is provided on the movable plate.

[0015] Optionally, the adjusting member includes:

[0016] A slide rail seat, wherein a T-shaped rail is arranged in the slide rail seat, and the L-shaped bracket is provided with a T-shaped slider assembled on the T-shaped rail;

[0017] A screw rod, the screw rod is rotatably disposed on the slide rail seat, and the L-shaped bracket is threadedly connected to the screw rod;

[0018] A knob is arranged at one end of the screw rod to rotate the screw rod.

[0019] Optionally, the needle holder comprises:

[0020] An I-shaped slider, the I-shaped slider slides along the L-shaped bracket, and the L-shaped bracket is provided with a sliding groove for the I-shaped slider to slide;

[0021] A tightening screw is arranged on the I-shaped slider and is used to fix the I-shaped slider at any position of the L-shaped bracket.

[0022] Compared with the prior art, the beneficial effects of this application are:

[0023] The embodiment of the present application proposes a device for collecting blood from the retrobulbar vein of mice. A flip movable plate is arranged on a bottom plate. The movable plate is erected when collecting blood so that the mouse can remain in a side-lying position. The left ear canal, right ear canal and incisors of the mouse are fixed by a first fixing member, a second fixing member and a third fixing member. Blood is collected by inserting a needle through a needle sleeve that forms a 45-degree angle with the surface formed by the first fixing member, the second fixing member and the third fixing member, thereby improving the accuracy of blood collection and needle insertion. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Schematic diagram of the structure of the mouse retrobulbar vein blood sampling device provided in the embodiment of the present application Figure 1 ;

[0025] Figure 2 Schematic diagram of the structure of the mouse retrobulbar vein blood sampling device provided in the embodiment of the present application Figure 2 ;

[0026] Figure 3 for Figure 1 Enlarged view of point A in the middle;

[0027] Figure 4 Schematic diagram of the structure of the mouse retrobulbar vein blood sampling device provided in the embodiment of the present application Figure 3 .

[0028] Description of the reference numerals in the accompanying drawings:

[0029] 1-base plate, 101-mounting seat, 2-movable plate, 201-hinge seat, 3-support rod, 301-outer rod, 302-inner rod, 4-L-shaped bracket, 5-slide groove, 6-adjusting piece, 601-track seat, 602-screw rod, 603-knob, 7-needle holder, 701-I-shaped slider, 702-tension screw, 703-needle sleeve, 8-blood collection needle. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0031] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0032] In this application, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0033] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present application, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0034] Refer to the attached Figures 1 to 4 The embodiment of the present application provides a mouse retrobulbar vein blood sampling device, comprising a base plate 1, a movable plate 2, a support rod 3, an L-shaped bracket 4 and a needle holder 7, wherein the base plate 1 is provided with four limb fixings for fixing the mouse's limbs, the movable plate 2 is flipped and arranged on the base plate 1, and the movable plate 2 is provided with a first fixing, a second fixing and a third fixing for fixing the mouse's left ear canal, right ear canal and incisor; one end of the support rod 3 is hinged to the base plate 1 and the other end is detachably connected to the movable plate 2, when not in use, the movable plate 2 is folded and attached to the base plate 1, when blood is collected, the movable plate 2 is in an upright state, and the support rod 3 erects the movable plate 2 to prevent it from tipping over; the L-shaped bracket 4 is arranged on a side of the movable plate 2 that is not connected to the base plate 1, and the L-shaped bracket 4 slides along the movable plate 2, and the needle holder 7 slides along the L-shaped bracket 4, and the moving direction of the L-shaped bracket 4 is perpendicular to the moving direction of the needle holder 7, the moving direction of the erected L-shaped bracket 4 is the X direction, and the moving direction of the needle holder 7 is the Y direction. The needle holder 7 is provided with a needle sleeve 703 for inserting the needle. In order to ensure the insertion angle of the needle, a reference plane is determined by the first fixing member, the second fixing member and the third fixing member (see the attached drawing). Figure 1 and attached Figure 2 The needle sleeve 703 forms an angle of 45 degrees with the reference plane, wherein the needle sleeve 703 forms an angle of 45 degrees with the reference plane. Figure 1 and attached Figure 2 The three points A, B and C mark the positions of the first fixing member, the second fixing member and the third fixing member respectively.

[0035] It can be found that the mouse retrobulbar vein blood sampling device provided in the embodiment of the present application, during use, by erecting the movable plate 2, the mouse is placed in a side-lying position on the bottom plate 1 and the movable plate 2. When the mouse is anesthetized, the limbs of the mouse are naturally hung on the bottom plate 1 due to the side-lying position, and are fixed by the four limb fixings on the bottom plate 1. When lying on the side, the back of the mouse's head is in a fit state with the movable plate 2. The left ear canal, right ear canal and incisors of the mouse are fixed by the first fixing member, the second fixing member and the third fixing member to achieve the purpose of restraining the mouse's head. By moving the L-shaped bracket 4 and the needle holder 7, the inner hole of the needle sleeve 703 is observed to see whether the needle sleeve 703 is aligned with the mouse's eyeball. When the needle sleeve 703 is aligned with the mouse's eyeball, the blood collection needle 8 is inserted into the needle sleeve 703 and inserted into the mouse's retrobulbar vein at a preset angle to complete the blood collection operation.

[0036] It should be noted that the limb fixing member, the first fixing member, the second fixing member and the third fixing member mentioned above are all conventional structures for fixing mice and belong to the prior art, so this application will not elaborate on this part in detail.

[0037] Specifically:

[0038] like Figures 1 to 4 As shown, a mounting seat 101 is mounted on the bottom plate 1, and end shafts are provided at both ends of the movable plate 2, and the movable plate 2 is rotatably connected to the mounting seat 101 through the end shafts, so that the movable plate 2 can be turned over and folded on the bottom plate 1. The length of the movable plate 2 is shorter than that of the bottom plate 1, so as to facilitate the blood collection operation.

[0039] In order to enable the support rod 3 to erect the movable board 2 in an upright state while not affecting the movable board 2 from being folded to fit with the bottom board 1, in a preferred embodiment, as Figure 4 As shown, the movable plate 2 is provided with a hinge seat 201 on the plate surface for connecting the support rod 3, and the hinge seat 201 is provided with a connecting pin hole. The support rod 3 includes an outer rod 301 and an inner rod 302, and one end of the inner rod 302 extends into the outer rod 301 to form a sliding connection, and the two remain fixed. A spring pin is installed at the other end of the inner rod 302. When the inner rod 302 is pressed into the hinge seat 201, the spring pin is in a compressed state. When the inner rod 302 is installed in place, the spring pin pops out and snaps into the connecting pin hole, thereby completing the connection between the support rod 3 and the movable plate 2. A placement groove is provided on the plate surface of the fixed plate, and the end of the outer rod 301 away from the inner rod 302 is rotated and installed in the placement groove. It is not difficult to imagine that since the outer rod 301 and the inner rod 302 are slidably connected to form a retractable structure, when not in use, the support rod 3 is in a retracted state and separated from the movable plate 2. At this time, the support rod 3 can be placed in the placement groove, and the movable plate 2 can be folded to fit with the bottom plate 1. When drawing blood, the support rod 3 is elongated and connected to the movable plate 2 to support the movable plate 2 in an upright state.

[0040] Furthermore, to realize the movement of the L-shaped bracket 4, as Figure 1 to Figure 2 As shown, the movable plate 2 is provided with an adjusting member 6 for adjusting the sliding of the L-shaped bracket 4, and the adjusting member 6 includes a slide rail seat, a screw rod 602 and a knob 603. The slide rail seat is integrally formed on the side of the movable plate 2 that is not connected to the bottom plate 1, and a T-shaped track is provided in the slide rail seat. The L-shaped bracket 4 is provided with a T-shaped slider assembled on the T-shaped track. The screw rod 602 is rotatably provided on the slide rail seat. The L-shaped bracket 4 is threadedly connected with the screw rod 602. One end of the screw rod 602 extends out of the slide rail seat and is fixedly connected to the knob 603. It can be found that by rotating the screw rod 602 by the knob 603, the L-shaped bracket 4 can reciprocate along the track seat 601.

[0041] In addition, such as Figure 1 and Figure 3 As shown, the frame body of the L-shaped bracket 4 perpendicular to the movable plate 2 is a telescopic structure, including two square tubes plugged into each other, and the positions of the two square tubes are fixed by spring pins. The extension and contraction of the L-shaped bracket 4 can make the blood collection needle approach the mouse. A slide groove 5 is arranged on the frame body of the L-shaped bracket 4 parallel to the movable plate 2, and the needle holder 7 slides along the slide groove 5. The needle holder 7 includes an I-shaped slider 701 and a tightening screw 702, the I-shaped slider is arranged in the slide groove 5, and the tightening screw 702 is arranged on the I-shaped slider 701. It can be imagined that the extension and contraction of the L-shaped bracket can make the blood collection needle 8 approach the mouse eyeball, and by tightening the screw 702 by turning the knob 603 outward, the tightening screw 702 is separated from the L-shaped bracket 4, so that the I-shaped slider 701 can be moved. On the contrary, by tightening the screw 702 by turning the knob 603 inward, the tightening screw 702 is pressed against the L-shaped bracket 4, thereby fixing the position of the I-shaped slider 701.

[0042] Furthermore, in the above, if Figure 3 As shown, the blood collection needle 8 includes a steel needle 801 and a hose 802 with a scale connected to the steel needle 801. The scale can be used to clearly indicate the amount of blood collected. When the amount of blood collected reaches the requirement, the steel needle can be pulled out to accurately collect blood so that the blood sample can be matched with the pre-prepared anticoagulant dose.

[0043] In summary, the mouse retrobulbar vein blood sampling device provided in the embodiment of the present application has the following usage method:

[0044] First, the movable plate 2 is rotated to an upright state, and the movable plate 2 is fixed by the support rod 3;

[0045] Next, the limbs, left ear canal, right ear canal and incisors of the anesthetized mouse are fixed by using the limb fixing device, the first fixing device, the second fixing device and the third fixing device, so that the mouse is fixed in a lateral position;

[0046] Then, the screw rod 602 is screwed and the I-shaped slider 701 is moved to align the needle sleeve 703 with the mouse eyeball, and the position of the needle sleeve 703 is fixed by tightening the screw 702, and the needle sleeve 703 is moved toward one side of the mouse through the L-shaped bracket 4, and the needle sleeve 703 is brought close to the mouse eyeball;

[0047] Finally, the blood collection needle 8 is inserted into the needle sleeve 703 and inserted into the posterior bulbar vein to complete the blood collection.

[0048] Of course, it is understandable that there may be many unexpected situations during the blood collection process, such as mice waking up during blood collection, mice dying, etc. The present application may also adopt the following coping settings in the above embodiments:

[0049] In order to solve the problem of mice waking up when anesthesia is about to end during the blood collection process, an external anesthesia tube can be connected. When the anesthesia is about to end, the mouse can continue to be anesthetized through the anesthesia tube. In addition, an anesthesia recovery chamber can be set up to wake the mouse after the blood collection is completed.

[0050] To address the problem of mouse death during blood collection, a heart rate monitor can be installed in the mouse's heart area. When the mouse's heart rate exceeds the recommended value, an alarm can be sounded in time and relevant emergency measures can be taken.

[0051] In addition, a micro-negative pressure device can be connected to the end of the hose of the blood collection needle to facilitate negative pressure blood collection.

[0052] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A mouse retrobulbar vein blood sampling device, characterized in that: include: A bottom plate, wherein limb fixing parts for fixing the limbs of the mouse are arranged on the bottom plate; A movable plate, the movable plate is flipped and arranged on the bottom plate, and the movable plate is provided with a first fixing member, a second fixing member and a third fixing member for fixing the left ear canal, the right ear canal and the incisor of the mouse; A support rod, one end of which is hinged to the bottom plate and the other end of which is detachably connected to the movable plate, and the support rod can erect the movable plate perpendicular to the bottom plate; An L-shaped bracket, the L-shaped bracket is arranged on a side of the movable plate that is not connected to the bottom plate, and the L-shaped bracket slides along the movable plate; The needle rack slides along the L-shaped bracket, and a needle sleeve for inserting the needle is arranged on the needle rack, and the needle sleeve forms an angle of 45 degrees with the plane defined by the first fixing member, the second fixing member and the third fixing member.

2. The mouse retrobulbar vein blood sampling device according to claim 1, characterized in that: The movable plate is provided with a hinge seat on the plate surface for connecting the support rod, the hinge seat is provided with a connecting pin hole, a spring pin is provided at one end of the support rod connected to the movable plate, the support rod is placed in the hinge seat and the spring pin is embedded in the connecting pin hole.

3. The mouse retrobulbar vein blood sampling device according to claim 2, characterized in that: The support rod comprises an outer rod and an inner rod, the outer rod and the inner rod are slidably connected, the outer sleeve is movably connected to the base plate, the inner rod is connected to the movable plate, and a placement groove for placing the support rod is provided on the base plate.

4. The mouse retrobulbar vein blood sampling device according to claim 1, characterized in that: The movable plate is provided with an adjusting member for adjusting the sliding movement of the L-shaped bracket.

5. The mouse retrobulbar vein blood sampling device according to claim 4, characterized in that: The adjusting member comprises: A slide rail seat, wherein a T-shaped rail is arranged in the slide rail seat, and the L-shaped bracket is provided with a T-shaped slider assembled on the T-shaped rail; A screw rod, the screw rod is rotatably disposed on the slide rail seat, and the L-shaped bracket is threadedly connected to the screw rod; A knob is arranged at one end of the screw rod to rotate the screw rod.

6. The mouse retrobulbar vein blood sampling device according to claim 1, characterized in that: The needle holder comprises: An I-shaped slider, the I-shaped slider slides along the L-shaped bracket, and the L-shaped bracket is provided with a sliding groove for the I-shaped slider to slide; A tightening screw is arranged on the I-shaped slider and is used to fix the I-shaped slider at any position of the L-shaped bracket.

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