A prescription composition and preparation method of plant extracts and trace elements for whitening and anti-aging

Abstract

A prescription composition and preparation method of plant extracts and trace elements that can be used for whitening and anti-aging. Through extraction, preparation, stability evaluation, microbiological experiments and basic pharmacodynamics experiments, it is proved that the ingredients in this plant essential oil are a natural progesterone supplement and estrogen substitute, which can enter the body through skin application to stimulate the secretion of estrogen in the human body, and at the same time increase the content of dehydroepiandrosterone (DHEA) in the body, so that the skin can achieve the effect of whitening and anti-aging.

Description

1. Technical Field

[0001] The invention relates to an essential oil composed of a plant extract and trace elements, and in particular to a prescription composition and a preparation method of the plant extract and trace elements which can be used for whitening and anti-aging. 2. Background Technology

[0002] The main active ingredient of Dioscorea sibiricum extract is diosgenin, a natural progesterone supplement and estrogen substitute. Exogenous supplementation and human estrogen play a two-way role in balancing hormones. It can be used in breast enhancement products to safely promote the development of breast tissue and make the skin full of vitality. In terms of skin care, it can increase the content of dehydroepiandrosterone (DHEA) in the body and delay aging; at the same time, it has soothing and anti-inflammatory effects.

[0003] This patent uses reed grass extract and vitamin C, glycerin, niacinamide, PEG-400, DL-α-tocopherol, 1,2-hexanediol and the like. Reed grass extract can effectively promote the secretion of estrogen in women, thereby achieving anti-aging effects, and ingredients such as niacinamide can achieve whitening effects. III. Summary of the invention

[0004] In order to meet the pursuit of whitening and anti-aging by the majority of women, the present invention provides a prescription composition and a preparation method of plant extracts and trace elements that can be used for whitening and anti-aging.

[0005] The technical solution adopted by the present invention is:

[0006] Prescription composition: Reed grass extract (main drug) 10%, sweet almond oil (oil phase) 58.5%, deionized water (excipient) 20%, glycerin (moisturizing, shrinking) 6%, niacinamide (whitening) 1%, PEG-400 (moisturizing) 3%, DL-α-tocopherol (anti-aging, antioxidant) 2%, 1,2-hexanediol (moisturizing, preservative) 0.5%

[0007] (1) Preparation of Reed Grass Essential Oil:

[0008] Stir and dissolve the reed grass extract in deionized water for later use, heat the sweet almond oil in a water bath to 70°C, add glycerol, PEG-400, DL-α-tocopherol and 1,2-hexanediol and stir until dissolved, emulsify with a high shear dispersing emulsifier after dissolution, add the reed grass solution, emulsify with a high shear dispersing emulsifier, cool and bottle.

[0009] The specific implementation of the present invention is described below with reference to examples. However, the protection scope of the present invention cannot be considered to be limited to the following specific implementation and specific examples. For ordinary technicians in the relevant technical field, several simple deductions or equivalent substitutions can be made without departing from the basic premise of the concept of the present invention, and these equivalent substitution schemes are still considered to be within the protection scope of the present invention. IV. Description of the drawings

[0010] Figure 1 Total colony count results

[0011] Figure 2 Thermotolerant E. coli test results

[0012] Figure 3 Pseudomonas aeruginosa test results

[0013] Figure 4 Staphylococcus aureus test results

[0014] Figure 5 Mold and yeast test results

[0015] Figure 6 Estrogen test results

[0016] Figure 7 SOD value test results

[0017] Figure 8 Skin pathology results of each group

[0018] The specific implementation of the present invention is described below with reference to examples. However, the protection scope of the present invention cannot be considered to be limited to the following specific implementation and specific examples. For ordinary technicians in the relevant technical field, several simple deductions or equivalent substitutions can be made without departing from the basic premise of the concept of the present invention, and these equivalent substitution schemes are still considered to be within the protection scope of the present invention. V. Specific implementation methods

[0019] The present invention is described in more detail below with reference to experimental examples and embodiments.

[0020] Example 1

[0021] Stir and dissolve the reed grass extract in deionized water for later use, heat the sweet almond oil in a water bath to 70°C, add glycerol, PEG-400, DL-α-tocopherol and 1,2-hexanediol and stir until dissolved, emulsify with a high shear dispersing emulsifier after dissolution, add the reed grass solution, emulsify with a high shear dispersing emulsifier, cool and bottle.

[0022] Experimental Example 1-Stability Evaluation of Reed Grass Essential Oil

[0023] Study on the stability of reed grass essential oil: stability experiments were conducted from the perspectives of cold resistance, heat resistance and centrifugation.

[0024] The results showed that the essential oil of Reed Grass had good stability.

[0025] Cold resistance experiment: Take two portions of Reed Grass essential oil, place one at room temperature and the other in a -8±2℃ refrigerator. Take them out after 24 hours, leave them at room temperature, and observe the phenomenon. The two samples have the same appearance and no stratification.

[0026] Heat resistance test: Take two portions of Reed Grass essential oil, place one at room temperature and the other in a constant temperature incubator at 40±1℃. Take them out after 24 hours, leave them at room temperature and observe the phenomenon. The two samples have the same appearance and no stratification.

[0027] Centrifugation experiment: Take a portion of the essential oil of Reed Grass, use a pipette to suck it into a centrifuge tube, centrifuge it at 2000r·min-1 for 30min, take it out, and observe the phenomenon of the emulsion. The appearance of the emulsion does not change, and there is no stratification, which proves that the centrifugal stability is good.

[0028] Experimental Example 1-Microbial Detection Test of Reed Grass Essential Oil

[0029] 1) Research purpose: To detect the colony count of various bacteria in the essential oil of Reed Grass

[0030] 2) Research methods: In accordance with the basic requirements for cosmetic microbiological testing stipulated in the "Technical Specifications for Safety of Cosmetics (2015 Edition)", the samples of the essential oil of the reed grass plant were collected, preserved and prepared for testing.

[0031] Weigh 10g of the plant essential oil prepared according to the formula, put it into a sterilized conical flask, and then inject 90mL of sterile saline. After standing, take the supernatant to obtain a 1:10 dilution test solution. Take 1mL of the 1:10 dilution test solution, add 9mL of sterile saline, and mix well to make a 1:100 dilution test solution.

[0032] Prepare lecithin Tween 80 nutrient agar medium by heating and boiling according to the method in the instructions for the culture medium reagent. After sterilization in an autoclave, cool to 45℃-50℃ and set aside. Use a pipette to respectively draw 1mL of the test solution with a dilution of 1:10 and 1mL of the test solution with a dilution of 1:100, and inject them into the empty plates that have been sterilized at high temperature. Then inject 15mL of lecithin Tween 80 nutrient agar medium into each plate. Before the culture medium solidifies, rotate the plate to mix the test solution and the culture medium thoroughly. Take another sterilized empty plate without the test solution sample, and add only 15mL of lecithin Tween 80 nutrient agar medium as a blank control. After the culture medium in the plate has solidified, mark it according to the group and place it in a 36±1°C incubator for 48±2h. Observe the phenomenon of the culture medium after 48 hours, such as Figure 1 shown.

[0033] 1.1 Detection of heat-resistant E. coli: Heat and boil the double lactose bile salt (containing neutralizer) medium according to the method in the medium reagent manual. After sterilization in an autoclave, cool and set aside. Use a pipette to draw 10mL of the 1:10 dilution test solution and inject it into two empty plates respectively. Then inject 10mL of double lactose bile salt medium into each plate. Place the two plates in a 44.5±0.5℃ incubator for 24 hours. If neither acid nor gas production occurs in the culture medium, continue to culture for 48 hours. If the above phenomenon still does not occur, it is reported as negative for heat-resistant coliform bacteria.

[0034] 1.2 Pseudomonas aeruginosa detection: Prepare SCDLP liquid culture medium by heating and boiling according to the method in the medium reagent manual, sterilize it in an autoclave, and cool it for later use. Use a pipette to draw 10mL of the test solution with a 1:10 dilution, and inject it into two empty plates respectively. Then inject 90mL of SCDLP liquid culture medium into each plate. Place the two plates in a 36±1℃ incubator. For 18-24h, if Pseudomonas aeruginosa grows, a thin bacterial film will appear on the surface of the culture medium, and the culture medium will turn yellow-green or blue-green.

[0035] 1.3 Staphylococcus aureus detection: Prepare SCDLP liquid culture medium and Baird Parker plate culture medium by heating and boiling according to the method in the medium reagent manual, sterilize in an autoclave, cool and set aside. First, perform the bacterial enrichment operation, use a pipette to draw 10mL of the test solution with a dilution of 1:10, and inject it into the plate. Then inject 90mL of SCDLP liquid culture medium into the plate and place it in a 36±1℃ incubator for 24±2h. Next, perform the separation operation. In the prepared bacterial enrichment culture solution, use an alcohol lamp to burn and sterilize the inoculation loop to take 1-2 loops of liquid, streak inoculate on the solidified Baird Parker plate culture medium, and place it in a 36±1℃ incubator for 48h. If there is Staphylococcus aureus in the test solution of cosmetics, round and smooth protrusions will be observed on the Baird Parker plate culture medium, with a gray to black color, surrounded by a turbid band, and a transparent band on the outer layer.

[0036] 1.4 Mold and yeast detection: Prepare the red bengal culture medium by heating and boiling according to the method in the medium reagent manual. After sterilization in an autoclave, cool to 45℃-50℃ and set aside. Use a pipette to take 1mL of the test solution with a dilution of 1:10 and 1mL of the test solution with a dilution of 1:100, and inject them into the empty plate after high temperature sterilization. Then inject 15mL of red bengal culture medium into each plate. After the culture medium solidifies, mark it according to the group and place it in a 28±2℃ incubator for 5 days. Observe the phenomenon of the culture medium after 5 days.

[0037] 3) Research results: Figure 1 It can be seen that there is no colony formation in the blank control group, one colony formation was observed in one of the plates in the 1:10 dilution test group, and no colony formation was observed in each plate in the 1:100 dilution sample group. According to the colony counting and reporting method, the average colony count of each dilution is less than 30, so the average colony count of the lowest dilution multiplied by the dilution multiple is calculated. Therefore, the total number of colonies in the reed grass essential oil is less than 10 cfu g -1 , in line with the requirements of the "Technical Specifications for Safety of Cosmetics".

[0038] 2.1 The results after cultivation are as follows Figure 2 As can be seen from the figure, the test solutions of the two plates produced neither acid nor gas, proving that no heat-resistant Escherichia coli grew in the essential oil of the reed grass.

[0039] 2.2 Test results Figure 3 As shown in the figure, no bacterial film appeared on the surface of the culture solution, and it did not turn green. This proves that there is no growth of Pseudomonas aeruginosa in the essential oil of the reed grass.

[0040] 2.3 Test results Figure 4As shown in the figure, the above phenomenon did not occur in the plate, proving that there was no growth of Staphylococcus aureus in the essential oil of the reed grass.

[0041] 2.4 Test results Figure 5 As shown in the figure, one colony was observed in one plate of the 1:10 dilution test group, and two colonies were observed in the other plate. No colony formation was observed in the 1:100 dilution test group plate. The calculation method of the average colony count of mold and yeast in the sample refers to the total colony count of the sample. According to calculations, the total colony count of mold and yeast in the reed grass essential oil is less than 15 cfu g -1 , in line with the "Technical Specifications for Cosmetic Safety" standards.

[0042] Experimental Example 2

[0043] (1) Research purpose: In vivo study of the essential oil of Reed Grass

[0044] (2) Research methods: Animal experiments were conducted by applying essential oils to the glands of 8-week-old female KM mice. After one month, arterial blood and skin tissue were collected for measurement.

[0045] Determination of biochemical indicators:

[0046] 1) Six hormone determination: Mouse serum was taken, and 9 times the volume of normal saline was added at a ratio of volume (mL) = 1:9, and six hormone determinations were performed according to the instructions of the kit.

[0047] 2) Determination of total superoxide dismutase (SOD) activity: Accurately weigh 0.1 g of mouse skin, add 9 volumes of physiological saline at a ratio of weight (g): volume (mL) = 1:9, prepare a 10% tissue homogenate under ice water bath conditions, centrifuge at 2500 g / min for 10 min, take the supernatant, and determine the superoxide dismutase activity in the mouse skin homogenate according to the instructions of the kit.

[0048] 3) H&E staining of skin tissue

[0049] Steps: sampling, fixation → repair tissue blocks → embedding → slicing → tissue sample dewaxing → tissue sample hydration → hematoxylin staining → differentiation, anti-blue → eosin staining → alcohol dehydration → air drying, neutral gum sealing → observation under microscope → analysis.

[0050] (1) Research results:

[0051] 1) If Figure 6It can be seen that the average estrogen level in the blank control group is 515.14 ng / ml, the average estrogen level in the saline group is 494.10 ng / ml, and the average estrogen level in the drug-treated group is 541.19 ng / ml. It can be seen that the essential oil of the reed grass plant can effectively promote the secretion of estrogen, thereby achieving the effect of anti-aging.

[0052] 2) If Figure 7 It can be seen that the SOD activity of the blank control group is 63.43%, the SOD activity of the saline group is 67.44%, and the SOD activity value of the drug group is 74.87%. It can be seen that the SOD activity value of the drug group is significantly higher than that of the blank control group and the saline group. It can be concluded that the anti-aging effect of the reed grass essential oil is significant.

[0053] 3) If Figure 8 It can be seen that the skin structure of mice in the normal group was discernible, the surface was rough, the dermis and epidermis were still distinguishable, the thickness of the epidermis was uneven, and there were slightly fewer subcutaneous fat, hair follicles, and ducts. The skin structure of mice in the reed grass essential oil group was complete, the surface was smooth, the boundary between the dermis and epidermis was clear, the epidermis was regular, the epidermis was thicker, the number of cell layers was more, and the arrangement was dense; subcutaneous fat, hair follicles, and ducts were abundant.

Claims

1. A prescription composition and preparation method of plant extracts and trace elements that can be used for whitening and anti-aging, comprising the following steps: (1) According to the proportion, the plant essential oil material comprises 10% of reed grass extract, 58% of sweet almond oil, 20% of deionized water, 6% of glycerol, 1% of niacinamide, 3% of PEG-400, 2% of DL-α-tocopherol and 1% of 1,2-hexanediol; (2) stirring and dissolving the reed grass extract in deionized water for later use, heating the sweet almond oil in a water bath to 70° C., adding glycerol, PEG-400, DL-α-tocopherol and 1,2-hexanediol and stirring until dissolved, emulsifying with a high shear dispersing emulsifier after dissolution, adding the reed grass solution, and emulsifying with a high shear dispersing emulsifier; (3) The preparation conditions include: the main drug is 10% of Reed Grass and 58% of sweet almond oil.

2. The preparation method according to claim 1, characterized in that: The main drug is 10% of the extract of Reed Grass.

3. An essential oil of Reed Grass obtained by the preparation method according to any one of claims 1 to 2, characterized in that: The concentration ratio of the reed grass extract and the sweet almond oil in the plant essential oil is: 10%-20%: 58%-68%.

4. A plant extract essential oil for whitening and anti-aging, comprising the plant extract and formula auxiliary materials described in claim 2 or 3.

5. A plant extract essential oil for whitening and anti-aging according to claim 4, characterized in that: Calculated by concentration ratio, it comprises 10% to 20% of the reed grass extract as claimed in claim 4 and 58% to 68% of sweet almond oil.

6. The plant extract essential oil for whitening and anti-aging according to claim 1, characterized in that: The auxiliary materials include sweet almond oil, glycerin, PEG-400, niacinamide, DL-α-tocopherol and 1,2-hexanediol.

7. A plant extract essential oil for whitening and anti-aging according to claim 6, characterized in that: The type of the essential oil is selected from lotion, essential oil, essence, cold cream, facial mask (emulsified), emulsified shampoo, bath oil, massage oil.

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