An anti-HPV carbomer hypochlorous acid gel and a preparation method thereof

Abstract

The invention relates to the technical field of medicines, and in particular to an anti-HPV carbomer hypochlorous acid gel and a preparation method thereof; the gel comprises carbomer, triethanolamine, a 560ppm hypochlorous acid solution, a first plant extract and a second plant extract, wherein the first plant extract is prepared by concentrating a mixed extract of phellodendron, sophora flavescens, honeysuckle, oldenlandia diffusa, smilax glabra, houttuynia cordata and brucea javanica, and the second plant extract is prepared by concentrating a mixed extract of purslane, turmeric and forsythia, the first plant extract has antiviral, anti-inflammatory, heat-clearing and detoxifying effects, the second plant extract has antiviral and anti-inflammatory effects, and can enhance immunity, and through the mixing of the above substances, the anti-HPV carbomer hypochlorous acid gel combines the gel matrix characteristics of carbomer, the strong oxidative bactericidal ability of hypochlorous acid and the antiviral and anti-inflammatory effects of a variety of plant extracts, thereby providing a safe and effective treatment option for HPV infected persons.

Description

Technical Field

[0001] The present invention relates to the technical field of medicines, and in particular to an anti-HPV carbomer hypochlorous acid gel and a preparation method thereof. Background Art

[0002] Papillomavirus (HPV) is a spherical DNA virus belonging to the papillomavirus family, the papillomavirus A genus, which can cause squamous epithelial proliferation in the skin and mucous membranes of the human body, manifesting as common warts, genital warts (condyloma acuminatum) and other symptoms.

[0003] HPV infects humans mainly through direct or indirect contact with contaminated objects or through sexual transmission. HPV infection is host and tissue specific and can only infect damaged cells of human skin and mucous membranes. After HPV infects these cells, it lurks in the basal cells in a small amount of free DNA state to achieve immune escape. As basal cells continue to differentiate, mature, and migrate to the surface of the mucosa, the HPV virus proliferates in large quantities. With the apoptosis of epithelial cells on the surface of the mucosa, a large number of viral particles are released onto the epithelial surface, serving as a new source of infection and further aggravating the infection.

[0004] Currently, preventive vaccines can effectively prevent HPV infection, but they have no obvious therapeutic effect on patients with existing HPV infection or precancerous lesions. Therefore, it is necessary to develop therapeutic drugs for HPV infection. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-HPV carbomer hypochlorous acid gel and a preparation method thereof, so as to solve the problem that the current preventive vaccine can effectively prevent HPV infection, but has no obvious therapeutic effect on patients with existing HPV infection or precancerous lesions, so it is necessary to develop therapeutic drugs for HPV infection.

[0006] To achieve the above object, the present invention provides an anti-HPV carbomer hypochlorous acid gel, wherein the anti-HPV carbomer hypochlorous acid gel comprises the following components in percentage by mass:

[0007] Carbomer 0.6-1.8%, triethanolamine 0.6-1.8%, 560ppm hypochlorous acid solution 0.2-0.6%, a first plant extract 3-9%, a second plant extract 3-9%, and the balance is purified water. The first plant extract is prepared by concentrating a mixed extract of Phellodendron chinense, Sophora flavescens, Flos lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica. The second plant extract is prepared by concentrating a mixed extract of Portulaca oleracea, Curcuma longa and Fructus Forsythiae.

[0008] The present invention also provides a method for preparing an anti-HPV carbomer hypochlorous acid gel, which is applied to the anti-HPV carbomer hypochlorous acid gel as described above, comprising the following steps:

[0009] Accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract;

[0010] Precisely weigh purslane, turmeric and forsythia to prepare a second plant extract;

[0011] Slowly add carbomer powder into an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution;

[0012] Use a dropper to slowly add triethanolamine to the carbomer solution, stirring while adding, and adjust the pH value to 6-7. The carbomer solution forms a transparent gel;

[0013] Add 560 ppm hypochlorous acid solution to the formed transparent gel and stir evenly with a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel;

[0014] Adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, and continuing to stir until uniform, to obtain a uniformly mixed gel;

[0015] The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to prepare an anti-HPV carbomer hypochlorous acid gel.

[0016] Among them, in the step of "accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare the first plant extract", the specific steps of preparing the first plant extract are:

[0017] After the weighed plant materials of Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container;

[0018] Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract;

[0019] The filtered extract is concentrated to obtain the first plant extract.

[0020] Wherein, in the step of “precisely weighing purslane, turmeric and forsythia to prepare a second plant extract”, the specific steps of preparing the second plant extract are:

[0021] After the weighed plant materials of purslane, turmeric and forsythia are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container;

[0022] Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract;

[0023] The filtered extract is concentrated to obtain the second plant extract.

[0024] Among them, in the step of "slowly adding carbomer powder to an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution", the stirring speed is set to 800-1000 rpm, and the stirring time is 30-40 minutes.

[0025] Among them, in the step of "adding 560 ppm hypochlorous acid solution to the formed transparent gel, stirring evenly with a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel", the stirring speed is set to 400-600 rpm, and the stirring time is 15-25 minutes.

[0026] Wherein, in the step of "adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution and continuing to stir until uniform", the stirring speed is set to 420-500 rpm, and the stirring time is 20-28 minutes.

[0027] The present invention discloses an anti-HPV carbomer hypochlorous acid gel and a preparation method thereof. The carbomer, as a gel matrix, provides a stable gel form. The triethanolamine adjusts the pH value of the gel to make it more suitable for skin use. The 560ppm hypochlorous acid solution has strong oxidizing properties and can effectively kill viruses and bacteria. The first plant extract has antiviral, anti-inflammatory, heat-clearing and detoxifying effects. The second plant extract has antiviral and anti-inflammatory effects and can enhance immunity. Through the mixing of the above substances, the anti-HPV carbomer hypochlorous acid gel combines the gel matrix characteristics of carbomer, the strong oxidizing and bactericidal ability of hypochlorous acid and the antiviral and anti-inflammatory effects of various plant extracts, thereby providing a safe and effective treatment option for HPV-infected persons. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 The present invention provides a flow chart of the steps of preparing the anti-HPV carbomer hypochlorous acid gel.

[0030] Figure 2 It is a flow chart of the steps of the method for preparing the anti-HPV carbomer hypochlorous acid gel according to the first embodiment of the present invention.

[0031] Figure 3 It is a flow chart of the steps of the method for preparing the anti-HPV carbomer hypochlorous acid gel according to the second embodiment of the present invention.

[0032] Figure 4 It is a flow chart of the steps of the method for preparing the anti-HPV carbomer hypochlorous acid gel according to the third embodiment of the present invention. DETAILED DESCRIPTION

[0033] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0034] The present invention provides an anti-HPV carbomer hypochlorous acid gel, which comprises the following components in percentage by mass:

[0035] Carbomer 0.6-1.8%, triethanolamine 0.6-1.8%, 560ppm hypochlorous acid solution 0.2-0.6%, a first plant extract 3-9%, a second plant extract 3-9%, and the balance is purified water. The first plant extract is prepared by concentrating a mixed extract of Phellodendron chinense, Sophora flavescens, Flos lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica. The second plant extract is prepared by concentrating a mixed extract of Portulaca oleracea, Curcuma longa and Fructus Forsythiae.

[0036] In this embodiment, the carbomer, as a gel matrix, provides a stable gel form, the triethanolamine adjusts the pH value of the gel to make it more suitable for skin use, the 560ppm hypochlorous acid solution has strong oxidizing properties and can effectively kill viruses and bacteria, the first plant extract has antiviral, anti-inflammatory, heat-clearing and detoxifying effects, and the second plant extract has antiviral and anti-inflammatory effects and can enhance immunity. Through the mixing of the above substances, the anti-HPV carbomer hypochlorous acid gel combines the gel matrix properties of carbomer, the strong oxidizing and bactericidal ability of hypochlorous acid and the antiviral and anti-inflammatory effects of various plant extracts, providing a safe and effective treatment option for HPV-infected people.

[0037] See also Figure 1 The present invention also provides a method for preparing an anti-HPV carbomer hypochlorous acid gel, which is applied to the anti-HPV carbomer hypochlorous acid gel as described above, comprising the following steps:

[0038] S1: accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract;

[0039] S2: accurately weighing purslane, turmeric and forsythia to prepare a second plant extract;

[0040] S3: Slowly add carbomer powder into an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution;

[0041] S4: Use a dropper to slowly add triethanolamine to the carbomer solution while stirring, and adjust the pH value to 6-7. The carbomer solution forms a transparent gel;

[0042] S5: Add 560 ppm hypochlorous acid solution to the formed transparent gel, and stir evenly using a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel;

[0043] S6: adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, and continuing to stir until uniform, to obtain a uniformly mixed gel;

[0044] S7: The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to prepare an anti-HPV carbomer hypochlorous acid gel.

[0045] In this embodiment, the carbomer, as a gel matrix, provides a stable gel form, the triethanolamine adjusts the pH value of the gel to make it more suitable for skin use, the 560ppm hypochlorous acid solution has strong oxidizing properties and can effectively kill viruses and bacteria, the first plant extract has antiviral, anti-inflammatory, heat-clearing and detoxifying effects, and the second plant extract has antiviral and anti-inflammatory effects and can enhance immunity. Through the mixing of the above substances, the anti-HPV carbomer hypochlorous acid gel combines the gel matrix properties of carbomer, the strong oxidizing and bactericidal ability of hypochlorous acid and the antiviral and anti-inflammatory effects of various plant extracts, providing a safe and effective treatment option for HPV-infected people.

[0046] Among them, in the step of "accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare the first plant extract", the specific steps of preparing the first plant extract are:

[0047] After the weighed plant materials of Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container;

[0048] Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract;

[0049] The filtered extract is concentrated to obtain the first plant extract.

[0050] Wherein, in the step of “precisely weighing purslane, turmeric and forsythia to prepare a second plant extract”, the specific steps of preparing the second plant extract are:

[0051] After the weighed plant materials of purslane, turmeric and forsythia are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container;

[0052] Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract;

[0053] The filtered extract is concentrated to obtain the second plant extract.

[0054] Among them, in the step of "slowly adding carbomer powder to an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution", the stirring speed is set to 800-1000 rpm, and the stirring time is 30-40 minutes.

[0055] Among them, in the step of "adding 560 ppm hypochlorous acid solution to the formed transparent gel, stirring evenly with a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel", the stirring speed is set to 400-600 rpm, and the stirring time is 15-25 minutes.

[0056] Wherein, in the step of "adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution and continuing to stir until uniform", the stirring speed is set to 420-500 rpm, and the stirring time is 20-28 minutes.

[0057] Embodiment 1:

[0058] See also Figure 2 The present invention also provides a method for preparing an anti-HPV carbomer hypochlorous acid gel, which is applied to the anti-HPV carbomer hypochlorous acid gel as described above, comprising the following steps:

[0059] S101: accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract;

[0060] S102: Accurately weigh purslane, turmeric and forsythia to prepare a second plant extract;

[0061] S103: slowly adding 0.6% carbomer powder into an appropriate amount of purified water, and during the adding process, stirring for 30 minutes at a speed of 800 rpm until the carbomer is completely dissolved to form a uniform carbomer solution;

[0062] S104: using a dropper, slowly add 0.6% triethanolamine to the carbomer solution while stirring, and adjust the pH value to 6, so that the carbomer solution forms a transparent gel;

[0063] S105: adding 0.2% 560ppm hypochlorous acid solution to the formed transparent gel, and stirring for 15 minutes with a stirrer at a speed of 400 rpm to ensure that the hypochlorous acid is evenly distributed in the gel;

[0064] S106: adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, stirring for 20 minutes at a rotation speed of 420 rpm to obtain a uniformly mixed gel;

[0065] S107: The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to obtain an anti-HPV carbomer hypochlorous acid gel.

[0066] Embodiment 2:

[0067] See also Figure 3 The present invention also provides a method for preparing an anti-HPV carbomer hypochlorous acid gel, which is applied to the anti-HPV carbomer hypochlorous acid gel as described above, comprising the following steps:

[0068] S201: accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract;

[0069] S202: accurately weighing purslane, turmeric and forsythia to prepare a second plant extract;

[0070] S203: slowly adding 1.2% carbomer powder into an appropriate amount of purified water, and during the adding process, stirring for 35 minutes at a speed set at 900 rpm until the carbomer is completely dissolved to form a uniform carbomer solution;

[0071] S204: using a dropper, slowly add 1.2% triethanolamine to the carbomer solution while stirring, and adjust the pH value to 6.5, so that the carbomer solution forms a transparent gel;

[0072] S205: adding 0.4% 560ppm hypochlorous acid solution to the formed transparent gel, and stirring for 20 minutes with a stirrer at a speed of 500 rpm to ensure that the hypochlorous acid is evenly distributed in the gel;

[0073] S206: adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, stirring for 24 minutes at a rotation speed of 460 rpm to obtain a uniformly mixed gel;

[0074] S207: The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to obtain an anti-HPV carbomer hypochlorous acid gel.

[0075] Embodiment 3:

[0076] See also Figure 4 The present invention also provides a method for preparing an anti-HPV carbomer hypochlorous acid gel, which is applied to the anti-HPV carbomer hypochlorous acid gel as described above, comprising the following steps:

[0077] S301: accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract;

[0078] S302: accurately weighing purslane, turmeric and forsythia to prepare a second plant extract;

[0079] S303: slowly adding 1.8% carbomer powder into an appropriate amount of purified water, and during the adding process, stirring for 40 minutes at a speed set at 1000 rpm until the carbomer is completely dissolved to form a uniform carbomer solution;

[0080] S304: using a dropper, slowly add 1.8% triethanolamine to the carbomer solution while stirring, and adjust the pH value to 7, so that the carbomer solution forms a transparent gel;

[0081] S305: adding 0.6% 560ppm hypochlorous acid solution to the formed transparent gel, and stirring for 25 minutes with a stirrer at a speed of 600 rpm to ensure that the hypochlorous acid is evenly distributed in the gel;

[0082] S306: adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, stirring for 28 minutes at a rotation speed of 500 rpm to obtain a uniformly mixed gel;

[0083] S307: The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to obtain an anti-HPV carbomer hypochlorous acid gel.

[0084] What is disclosed above is only a preferred embodiment of the present invention, and it certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.

Claims

1. An anti-HPV carbomer hypochlorous acid gel, characterized in that: Includes the following components in percentage by mass: Carbomer 0.6-1.8%, triethanolamine 0.6-1.8%, 560ppm hypochlorous acid solution 0.2-0.6%, a first plant extract 3-9%, a second plant extract 3-9%, and the balance is purified water. The first plant extract is prepared by concentrating a mixed extract of Phellodendron chinense, Sophora flavescens, Flos lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica. The second plant extract is prepared by concentrating a mixed extract of Portulaca oleracea, Curcuma longa and Fructus Forsythiae.

2. A method for preparing an anti-HPV carbomer hypochlorous acid gel, applied to the anti-HPV carbomer hypochlorous acid gel as claimed in claim 1, characterized in that: The steps include: Accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract; Precisely weigh purslane, turmeric and forsythia to prepare a second plant extract; Slowly add carbomer powder into an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution; Use a dropper to slowly add triethanolamine to the carbomer solution, stirring while adding, and adjust the pH value to 6-7. The carbomer solution forms a transparent gel; Add 560 ppm hypochlorous acid solution to the formed transparent gel and stir evenly with a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel; Adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution, and continuing to stir until uniform, to obtain a uniformly mixed gel; The uniformly mixed gel is homogenized by a homogenizer to eliminate bubbles and particles, and then filled into a sterile container to prepare an anti-HPV carbomer hypochlorous acid gel.

3. The method for preparing the anti-HPV carbomer hypochlorous acid gel according to claim 2, characterized in that: In the step of "accurately weighing Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica to prepare a first plant extract", the specific steps of preparing the first plant extract are: After the weighed plant materials of Phellodendron amurense, Sophora flavescens, Flos Lonicerae, Hedyotis diffusa, Smilax glabra, Houttuynia cordata and Brucea javanica are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container; Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract; The filtered extract is concentrated to obtain the first plant extract.

4. The method for preparing the anti-HPV carbomer hypochlorous acid gel according to claim 3, characterized in that: In the step of "accurately weighing purslane, turmeric and forsythia to prepare a second plant extract", the specific steps of preparing the second plant extract are: After the weighed plant materials of purslane, turmeric and forsythia are crushed to an appropriate particle size, the crushed plant materials are placed in an extraction container; Adding an appropriate amount of ethanol solvent into the extraction container, heating the extraction container to boil the solvent, and performing extraction. After the extraction is completed, filtering the extract to remove plant residues to obtain a clarified extract; The filtered extract is concentrated to obtain the second plant extract.

5. The method for preparing the anti-HPV carbomer hypochlorous acid gel according to claim 4, characterized in that: In the step of "slowly adding carbomer powder to an appropriate amount of purified water, stirring while adding, until the carbomer is completely dissolved to form a uniform carbomer solution", the stirring speed is set to 800-1000 rpm, and the stirring time is 30-40 minutes.

6. The method for preparing the anti-HPV carbomer hypochlorous acid gel according to claim 5, characterized in that: In the step of "adding 560 ppm hypochlorous acid solution to the formed transparent gel, stirring evenly with a stirrer to ensure that the hypochlorous acid is evenly distributed in the gel", the stirring speed is set to 400-600 rpm, and the stirring time is 15-25 minutes.

7. The method for preparing the anti-HPV carbomer hypochlorous acid gel according to claim 6, characterized in that: In the step of "adding the prepared first plant extract and the second plant extract to the carbomer hypochlorous acid solution and continuing to stir until uniform", the stirring speed is set to 420-500 rpm, and the stirring time is 20-28 minutes.

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