Lactic acid bacteria private part care preparation and preparation method thereof

Abstract

The invention discloses a lactic acid bacteria private part care preparation and a preparation method thereof, and belongs to the technical field related to pharmaceutical preparations, the lactic acid bacteria private part care preparation comprises active ingredients and pharmaceutically acceptable auxiliary materials, the active ingredients comprise lactic acid bacteria, dandelion extract, malic acid and lactic acid, the lactic acid bacteria are at least one of lactobacillus crispatus, lactobacillus acidophilus, lactobacillus plantarum and bifidobacterium bifidum, the dandelion extracting solution contained in the nursing preparation further contains polysaccharide, nutrients can be provided for the lactic acid bacteria, the polysaccharide is generated when the polysaccharide is decomposed, active ingredients in dandelions are prevented from being oxidized and damaged, the activity is maintained for a long time, and the nursing preparation is suitable for being used for nursing the human body. The long-time antibacterial effect can be kept, and the continuous stability of the pudendum micro-ecological environment is promoted.

Description

Technical Field

[0001] The invention belongs to the technical field related to pharmaceutical preparations, and in particular relates to a lactic acid bacteria vaginal care preparation and a preparation method thereof. Background Art

[0002] The pathogenesis of vaginitis is mainly related to the imbalance of local flora in the vagina. In a normal vagina, there are many flora such as lactobacillus, streptococcus, and Staphylococcus epidermidis. These species maintain a certain proportion and maintain the ecological balance in the vagina. Low immunity, endocrine changes, and physical and mechanical damage to the human body destroy the ecological balance of the vagina, often causing vaginal infection and inflammation.

[0003] Existing private washes or gels, when used, will destroy the ecological environment of the genitals. Although they inhibit the growth of bacteria, they cannot regulate the ecological environment of the genitals. In addition, the antibacterial washes will also induce drug resistance of various pathogens of vaginitis, further leading to the inability to improve and restore the microecological environment. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a lactic acid bacteria vaginal care preparation and a preparation method thereof, wherein the dandelion extract contained in the care preparation also contains polysaccharides, which can provide nutrients for lactic acid bacteria, and the polysaccharides are produced when the polysaccharides are decomposed, so that the active ingredients in the dandelions are prevented from being destroyed by oxidation and maintain their activity for a long time, that is, they can maintain a long-term antibacterial effect and promote the continuous stability of the vaginal microecological environment.

[0005] The technical solution adopted by the present invention is as follows:

[0006] In the first aspect, the present invention proposes a lactic acid bacteria vaginal care preparation, comprising an active ingredient and a pharmaceutically acceptable excipient, wherein the active ingredient comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and the lactic acid bacteria is at least one of Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus plantarum and Bifidobacterium bifidum.

[0007] Furthermore, the total number of viable lactic acid bacteria is ≥ 5x10 3 CFU / g. (Content in 100ml dandelion extract)

[0008] Furthermore, the pH value of the preparation is 4.5-6.

[0009] Furthermore, the preparation is in the form of suppository, lotion or gel.

[0010] Furthermore, when the preparation is a gel, the auxiliary material is a temperature-sensitive gel; when the preparation is a lotion, the auxiliary material is water; when the preparation is a suppository, the auxiliary material is a suppository base.

[0011] In a second aspect, the present invention provides a method for preparing the lactic acid bacteria vaginal care preparation provided in the first aspect, the preparation method comprising the following steps:

[0012] Step 1: preparing a dandelion extract; the dandelion extract has a polysaccharide content of 55-65%, and a phenolic acid content of 7-10% such as caffeic acid and chlorogenic acid;

[0013] Step 2: preparing a mixture of malic acid and lactic acid;

[0014] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0015] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 4.5-6.

[0016] Step 5: Add auxiliary materials to the mixed solution prepared in step 4 to prepare corresponding dosage forms.

[0017] After adopting the above structure, the beneficial effects of the present invention are as follows: a mixed solution prepared by corresponding concentrations of malic acid and lactic acid can be used as a pH regulator to adjust the pH value of the preparation. In addition, malic acid can be used as an antioxidant to maintain the activity of the active ingredients in the dandelion extract, so that the active ingredients cooperate with lactic acid bacteria to act on the genital microenvironment together; in terms of the synergistic effect of dandelion and lactic acid bacteria, the active ingredient polysaccharide in the dandelion extract can provide "nutrients" for lactic acid bacteria, maintain the activity of lactic acid bacteria, and have a quantitative regulating effect on normal flora, especially lactic acid bacteria, and effectively supplement lactic acid bacteria in the genitals, so as to achieve antibacterial effect and maintain the long-term stability of the genital microenvironment; lactic acid bacteria decompose macromolecules during metabolism, or release the dandelion active ingredients from the cell wall through deglycosylation, thereby increasing the content of active ingredients in the dandelion extract, especially the antibacterial components of phenolic acid compounds (caffeic acid, chlorogenic acid, etc.) in the dandelion extract, and enhancing the effect of the active ingredients of the dandelion extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0019] Figure 1 It is a bar graph of Candida albicans inhibition rate in Example 14;

[0020] Figure 2 It is a bar graph of the antibacterial rate of Escherichia coli in Example 14;

[0021] Figure 3This is a bar graph of the inhibition rate of Staphylococcus aureus in Example 14. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0024] The microorganisms involved in the present invention are all from the China Microbiological Culture Collection Center, the methods not specifically described in the present invention are all conventional methods in the art, and the reagents not specifically described are all conventional reagents in the art.

[0025] Example 1

[0026] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0027] Step 1: Prepare dandelion extract:

[0028] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0029] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0030] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0031] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0032] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0033] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0034] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 4.5-6.

[0035] Step 5: The mixed solution obtained in step 4 is mixed with chitosan hydrogel at a ratio of 1: (15-20) to prepare a nursing gel preparation.

[0036] Example 2

[0037] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0038] Step 1: Prepare dandelion extract:

[0039] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0040] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0041] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0042] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0043] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0044] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0045] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 5.

[0046] Step 5: The mixed solution obtained in step 4 is mixed with chitosan hydrogel at a ratio of 1: (15-20) to prepare a nursing gel preparation.

[0047] Example 3

[0048] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0049] Step 1: Prepare dandelion extract:

[0050] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0051] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0052] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0053] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0054] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0055] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0056] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 5.5.

[0057] Step 5: The mixed solution obtained in step 4 is mixed with chitosan hydrogel at a ratio of 1: (15-20) to prepare a nursing gel preparation.

[0058] Example 4

[0059] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0060] Step 1: Prepare dandelion extract:

[0061] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0062] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0063] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0064] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0065] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0066] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0067] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 6.

[0068] Step 5: The mixed solution obtained in step 4 is mixed with chitosan hydrogel at a ratio of 1: (15-20) to prepare a nursing gel preparation.

[0069] Example 5

[0070] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0071] Step 1: Prepare dandelion extract:

[0072] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0073] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0074] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0075] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0076] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0077] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0078] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 4.5.

[0079] Step 5: Mix the mixed solution obtained in step 4 with the molten matrix at a ratio of 1: (15-20), inject into a mold, cool and solidify, and prepare a nursing suppository.

[0080] Example 6

[0081] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0082] Step 1: Prepare dandelion extract:

[0083] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0084] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0085] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0086] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0087] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0088] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0089] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 5.

[0090] Step 5: Mix the mixed solution obtained in step 4 with the molten matrix at a ratio of 1: (15-20), inject into a mold, cool and solidify, and prepare a nursing suppository.

[0091] Example 7

[0092] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0093] Step 1: Prepare dandelion extract:

[0094] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0095] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0096] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0097] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0098] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0099] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0100] Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 5.5.

[0101] Step 5: Mix the mixed solution obtained in step 4 with the molten matrix at a ratio of 1: (15-20), inject into a mold, cool and solidify, and prepare a nursing suppository.

[0102] Example 8

[0103] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0104] Step 1: Prepare dandelion extract:

[0105] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0106] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0107] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0108] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0109] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0110] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0111] Step 4: slowly adding the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjusting the pH value of the mixture to 6;

[0112] Step 5: Mix the mixed solution obtained in step 4 with the molten matrix at a ratio of 1: (15-20), inject into a mold, cool and solidify, and prepare a nursing suppository.

[0113] Example 9

[0114] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0115] Step 1: Prepare dandelion extract:

[0116] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0117] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0118] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0119] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0120] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0121] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0122] Step 4: slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 4.5;

[0123] Step 5: Mix the mixed solution obtained in step 4 with water at a ratio of 1: (15-20), and dilute to prepare a care solution.

[0124] Example 10

[0125] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0126] Step 1: Prepare dandelion extract:

[0127] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0128] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0129] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0130] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0131] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0132] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0133] Step 4: slowly adding the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjusting the pH value of the mixture to 5;

[0134] Step 5: Mix the mixed solution obtained in step 4 with water at a ratio of 1: (15-20), and dilute to prepare a care solution.

[0135] Embodiment 11

[0136] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0137] Step 1: Prepare dandelion extract:

[0138] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0139] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0140] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0141] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0142] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0143] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0144] Step 4: slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 5.5;

[0145] Step 5: Mix the mixed solution obtained in step 4 with water at a ratio of 1: (15-20), and dilute to prepare a care solution.

[0146] Example 12

[0147] A lactic acid bacteria genital care preparation comprises lactic acid bacteria, dandelion extract, malic acid and lactic acid, and is prepared by the following method:

[0148] Step 1: Prepare dandelion extract:

[0149] S1, crushing dandelion, adding deionized water (to make) at a solid-liquid ratio of 1:15, and performing microwave extraction to obtain an extract;

[0150] S2, the extract is filtered using filter paper and then filtered again using a microporous membrane to remove small particles;

[0151] S3, concentrating the filtrate using a nanofiltration membrane to obtain a concentrated solution;

[0152] S4, after the concentrated solution is sterilized, a dandelion extract is obtained.

[0153] Step 2: prepare a mixture of 1% malic acid and 86% lactic acid; prepare the mixture of malic acid and lactic acid in a volume ratio of 0.5:2, and stir evenly for standby use;

[0154] Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g;

[0155] Step 4: slowly adding the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjusting the pH value of the mixture to 6;

[0156] Step 5: Mix the mixed solution obtained in step 4 with water at a ratio of 1: (15-20), and dilute to prepare a care solution.

[0157] Example 13: Analysis of the content of quality markers in dandelion extract

[0158] The quality markers in the dandelion extract prepared in the above example were analyzed. Specifically, the anthrone-concentrated sulfuric acid method and ultra-high performance liquid chromatography were used to determine the contents of dandelion polysaccharides and phenolic acids (chlorogenic acid and caffeic acid) in the prepared dandelion extract, and the determined contents were 59% and 9.5%, respectively.

[0159] The polysaccharides in dandelion extract, including monosaccharides such as D-rhamnose, glucose, D-galactose, D-xylose and D-arabinose, can provide "nutrients" for lactic acid bacteria; phenolic acids mainly include chlorogenic acid and caffeic acid. Dandelion chlorogenic acid can play a synergistic role in antioxidant activity. Phenolic acids dominated by chlorogenic acid and caffeic acid have inhibitory effects on various bacteria, fungi and viruses, and can destroy the cell wall of bacteria, causing the contents of the bacteria to leak out, and then destroy the cell membrane structure of the bacteria, leading to cell death.

[0160] Example 14: Antibacterial experiment of nursing preparation

[0161] The experimental groups are as follows:

[0162] 1. The mixed solution obtained in step 4 of Example 1-4 has a total number of viable lactic acid bacteria of 5×10 3 CFU / g; respectively counted as experimental groups 1-4;

[0163] 2. The mixed solution obtained in step 4 of Example 1-4 has a total number of viable lactic acid bacteria of 1×10 4 CFU / g; respectively counted as experimental groups 5-8;

[0164] 3. The mixed solution obtained in step 4 of Example 1-4 has a total number of viable lactic acid bacteria of 1×10 3 CFU / g; counted as comparison groups 1-4 respectively;

[0165] 4. The total number of viable lactic acid bacteria is 5x10 3 CFU / g and 1x 10 4 CFU / g of bacterial agent was counted as control group 5-6;

[0166] 5. Prepare the dandelion extracts in Examples 1-4, and slowly add the malic acid and lactic acid mixtures prepared in step 2 of the corresponding examples, and adjust the pH values ​​of the mixtures to 4.5, 5, 5.5, and 6, respectively; counted as comparative groups 7-10;

[0167] ① Add 2×10 6 CFU / mL Candida albicans seed solution, and an equal amount of ultrapure water was set as a blank control group; after drug treatment at 30°C for 3 hours, the antibacterial effects of the above experimental group and control group on Candida albicans were explored by colony dilution plate counting;

[0168] ② Add 2×10 6 CFU / mL Escherichia coli seed solution, and an equal amount of ultrapure water was set as a blank control group;

[0169] ③ Add 2×10 6 CFU / mL Staphylococcus aureus seed solution, and an equal amount of ultrapure water was set as a blank control group;

[0170] Preparation of Escherichia coli and Staphylococcus aureus seed solution: inoculate the strain to be tested into LB medium and shake at 37°C until OD 600 = 0.6, diluted to 2 × 10 in fresh LB medium 6 CFU / mL.

[0171] Preparation of Candida albicans seed solution: Inoculate the Candida albicans to be tested into Sabouraud liquid medium and shake until OD 600 = 0.6, dilute with culture medium to 2 × 10 6 CFU / mL.

[0172] It should be noted that the Escherichia coli, Coccus aureus and Candida albicans involved in the antibacterial experiment are clinically extracted strains of relevant patients.

[0173] Antibacterial effect analysis reference Figure 1-3 and Table 1. The experimental groups 1-8 had significant antibacterial effects on the three strains, and were better than the antibacterial effects of the comparison groups 1-6 and 7-10. The comparison groups 5-6 and 7-10 all had antibacterial effects, but the antibacterial effects were significantly different from those of the experimental groups 1-8. In addition, the antibacterial effect of the mixed solution was better than that of the lactic acid bacteria solution alone and the mixed solution without lactic acid bacteria, indicating that the lactic acid bacteria and the dandelion extract had a synergistic effect to enhance the antibacterial effect.

[0174] Among them, the mixed solution has the best antibacterial effect on Escherichia coli at a pH of 5.5 and 5x10 3 CFU / g, it has the best antibacterial effect on Candida albicans, Escherichia coli and Staphylococcus aureus.

[0175] Table 1

[0176]

[0177] Although the embodiments of the present invention have been shown and described, it is understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the attached claims and their equivalents. In short, if those skilled in the art are inspired by it, without departing from the purpose of the invention, they can creatively design structural methods and embodiments similar to the technical solution, which should all fall within the scope of protection of the present invention. Each component of the present application can be driven by a corresponding external motor, which is a prior art and will not be elaborated in this application.

Claims

1. A lactic acid bacteria vagina care preparation, characterized in that: The invention comprises active ingredients and pharmaceutically acceptable adjuvants. The active ingredients comprise lactic acid bacteria, dandelion extract, malic acid and lactic acid. The lactic acid bacteria is at least one of Lactobacillus crispatus, Lactobacillus acidophilus, Lactobacillus plantarum and Bifidobacterium bifidum.

2. A lactic acid bacteria vagina care preparation according to claim 1, characterized in that: The total number of viable lactic acid bacteria is ≥1x10 CFU / g.

3. A lactic acid bacteria vagina care preparation according to claim 1, characterized in that: The pH value of the preparation is 4.5-6.

4. A lactic acid bacteria vagina care preparation according to claim 1, characterized in that: The preparation is in the form of a suppository, a lotion or a gel.

5. A lactic acid bacteria vagina care preparation according to any one of claims 1 to 3, characterized in that: When the preparation is a gel, the auxiliary material is a gel base; when the preparation is a lotion, the auxiliary material is water; when the preparation is a suppository, the auxiliary material is a suppository base.

6. A method for preparing a lactic acid bacteria vaginal care preparation according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: Step 1: preparing dandelion extract; Step 2: preparing a mixture of malic acid and lactic acid; Step 3: Add a predetermined amount of lactic acid bacteria to the dandelion extract prepared in step 1, so that the total number of viable lactic acid bacteria in 100 ml of the mixed solution is ≥ 5x10 3 CFU / g; Step 4: Slowly add the malic acid and lactic acid mixture prepared in step 2 to the mixture prepared in step 3, and adjust the pH value of the mixture to 4.5-6. Step 5: Add auxiliary materials to the mixed solution prepared in step 4 to prepare corresponding dosage forms.

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