Traditional Chinese medicine composition for preventing and treating calf mixed infection pneumonia and application

Abstract

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for preventing and treating calf mixed infection pneumonia and application, and the traditional Chinese medicine composition is prepared from, by weight, 30-40 parts of herba ephedrae, 20-30 parts of radix bupleuri, 20-30 parts of radix codonopsis, 20-30 parts of herba artemisiae annuae, 10-20 parts of fructus forsythiae, 10-20 parts of radix angelicae sinensis and 10-20 parts of radix puerariae. The traditional Chinese medicine composition can be used for treating calf mixed infection pneumonia, the highest cure rate reaches 100%, and meanwhile, the traditional Chinese medicine composition is small in toxic and side effects, low in cost and not prone to drug resistance, and has wide application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for preventing and treating mixed infection pneumonia in calves and an application thereof. Background Art

[0002] Calf pneumonia is a common and frequent respiratory disease in calves. It can occur throughout the year, and is more common in winter, early spring and late autumn, especially in calves from birth to 2 months old, with an infection rate of more than 50%. Calf pneumonia is also one of the most serious respiratory diseases in calves. Infected calves will cough, breathe faster, have difficulty breathing and have a fever. Sick calves will show symptoms such as depression, lethargy, slow reaction to the surrounding environment, and unwillingness to move. It seriously affects the growth and development of newborn calves, reduces their resistance, and reduces their production performance. If it is not treated effectively in time, it will cause the death of the calf.

[0003] Generally speaking, calf pneumonia is not caused by a single pathogenic microorganism, but by multiple pathogens, including viruses, bacteria, mycoplasma infections, etc. Mixed pneumonia in calves is a disease that is more complicated to diagnose and treat. During diagnosis, veterinarians make a preliminary judgment on whether the calf has pneumonia by observing symptoms such as coughing, abnormal breathing, fever, mental state and changes in appetite. At the same time, auscultation of the lungs can reveal abnormal respiratory sounds, such as rales and wheezing, which helps in the diagnosis of pneumonia. In addition, the pathogen can be determined by blood tests, pathogen isolation and identification, etc.

[0004] At present, antibiotics are the first drugs used in clinical treatment of calf pneumonia, such as penicillin, streptomycin, tetracycline, chloramphenicol, etc. However, the above drugs can treat a range of pathogens and cannot be used universally for all pathogens. For calves with mixed infections, many drugs need to be compounded for use. On the one hand, too high a dose of the drug will cause toxicity, and on the other hand, large-scale and long-term use of antibiotics will produce drug resistance, reducing the efficacy. Summary of the invention

[0005] In view of the above technical problems, the present invention provides a traditional Chinese medicine composition for preventing and treating mixed pneumonia in calves, wherein the traditional Chinese medicine composition comprises 30-40 parts of ephedra, 20-30 parts of bupleurum, 20-30 parts of codonopsis, 20-30 parts of artemisia annua, 10-20 parts of forsythia, 10-20 parts of angelica, and 10-20 parts of kudzu root.

[0006] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30-40 parts of ephedra, 20-30 parts of bupleurum, 20-30 parts of codonopsis, 20-30 parts of artemisia annua, 10-20 parts of forsythia, 10-20 parts of angelica, and 10-20 parts of kudzu root.

[0007] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 20 parts of bupleurum, 30 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 10 parts of angelica, and 10 parts of kudzu root.

[0008] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of ephedra, 20 parts of bupleurum, 20 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 10 parts of angelica, and 10 parts of kudzu root.

[0009] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 30 parts of bupleurum, 30 parts of codonopsis, 30 parts of artemisia annua, 20 parts of forsythia, 20 parts of angelica, and 20 parts of kudzu root.

[0010] Preferably, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 30 parts of bupleurum, 30 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 20 parts of angelica, and 10 parts of kudzu root.

[0011] The second object of the present invention is to provide a method for preparing the Chinese medicine composition, comprising the following steps: mixing the formula amounts of ephedra, bupleurum, codonopsis, artemisia annua, forsythia, angelica and kudzu root, adding water and boiling to obtain the composition.

[0012] The third object of the present invention is to provide the use of the traditional Chinese medicine composition in the preparation of medicines for preventing and treating mixed pneumonia in calves.

[0013] The fourth object of the present invention is to provide the use of the traditional Chinese medicine composition in the preparation of a feed additive for preventing and treating mixed pneumonia in calves.

[0014] The fifth object of the present invention is to provide a feed additive prepared by the traditional Chinese medicine composition.

[0015] Explanation of the prescription: Ephedra is warm in nature, pungent and slightly bitter in taste, and belongs to the lung and bladder meridians. Its pungent and warm nature can open the pores of the skin and promote the discharge of sweat. Ephedra belongs to the lung meridian and can enter the qi of the lung meridian, which has the effect of promoting the flow of lung qi. When treating coughs and asthma caused by stagnation of lung qi, ephedra can promote the flow of lung qi and restore normal respiratory function. Ephedra can promote lung qi upward, transport it to the bladder downward, regulate the water channels, and help the metabolism of water in the body. It is the main medicine. Codonopsis is sweet in taste and neutral in nature, and belongs to the spleen and lung meridians. It has the effects of replenishing the middle qi, strengthening the spleen and benefiting the lungs, and can not only replenish the lung qi, but also strengthen the spleen and stomach. It has a therapeutic effect on symptoms such as insufficient lung qi, coughing and shortness of breath, and low voice. By replenishing lung qi, the respiratory function and the function of protecting the lungs are enhanced, and the body's resistance is improved. At the same time, since the spleen is the mother of the lungs, strengthening the spleen also helps to enrich the lung qi. It is a minister medicine. Bupleurum is slightly cold in nature, bitter and pungent in taste, and enters the liver, gallbladder, and lung meridians. It has the effects of relieving fever, soothing the liver and relieving depression, and raising yang energy. It is slightly cold in nature, can disperse and reduce fever, and resolve the external pathogens from the surface. Bupleurum enters the liver meridian, is good at regulating liver qi, and has the effect of soothing the liver and relieving depression. It is a ministerial drug. Forsythia suspensa: bitter, slightly cold, enters the lung, heart, and small intestine meridians. It has the effects of clearing heat and detoxifying, reducing swelling and dispersing knots. It is used for wind-heat colds, the initial stage of febrile diseases, and warm and hot entering the camp. It is an adjuvant. Artemisia annua: bitter, pungent, and cold. It enters the liver and gallbladder meridians. It has the effects of clearing heat and relieving summer heat. It is an adjuvant. Angelica sinensis is warm in nature, sweet and pungent in taste, and enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. It is sweet, warm, and moist, and is good at nourishing the camp and blood. Angelica sinensis is pungent and warms and has the effect of activating blood circulation and removing blood stasis. It is a guiding drug. Pueraria root is cool in nature, sweet and pungent in taste, and enters the spleen and stomach meridians. It is a commonly used medicine for treating exogenous fever, especially when the symptoms of the exterior have not been resolved and the pathogenic heat has entered the interior. Pueraria root has a therapeutic effect on symptoms such as loss of body fluids due to fever, thirst and excessive drinking. It is a guiding drug.

[0016] The beneficial effects of the present invention are as follows: the present invention provides a traditional Chinese medicine composition for preventing and treating mixed pneumonia in calves, the traditional Chinese medicine composition can treat mixed pneumonia in calves, and the cure rate can reach up to 100%. At the same time, the traditional Chinese medicine composition has small toxic and side effects, low cost, is not easy to produce drug resistance, and has broad application prospects. DETAILED DESCRIPTION

[0017] The following examples are provided to facilitate a better understanding of the present invention, but are not intended to limit the present invention.

[0018] The experimental methods in the following examples are all conventional methods unless otherwise specified.

[0019] Unless otherwise specified, the test materials used in the following examples can be purchased on the market.

[0020] In the following examples, the modified Martin medium (containing phenol red) used has the following components: peptone 10 g / L, glucose 40 g / L, red Bengal (red tiger) 0.1 g / L, streptomycin 0.1 g / L, chloramphenicol 0.15 g / L, phenol red 0.02 g / L, agar 20 g / L.

[0021] In the following examples, the Chinese medicine compositions used are all decoctions of Chinese medicine compositions, which are obtained by taking medicines in proportion, soaking for half an hour, and boiling for one hour.

[0022] Serum samples were collected from Wuwei area in 2022-2023. The collection criteria were depression, cough, shortness of breath, tearing, excessive nasal secretions and other clinical manifestations. A total of 854 samples were collected in 2 years. The blood samples were centrifuged at 2500rpm for 10min. After the centrifugation, the serum was obtained and stored in a low-temperature refrigerator. Nasal swab samples were collected from some calves. After being sent to the laboratory, the samples were stored at -80℃ for subsequent pathogen detection.

[0023] Laboratory tests showed that most calves with pneumonia were infected with mycoplasma, viruses and bacteria. The pathogens involved included bovine viral diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBRV), Pasteurella multocida (Pm), Mannheimia hemolytica (Mh), bovine coronavirus (BCOV), bovine parainfluenza virus type 3 (BPIV3) and bovine respiratory syncytial virus. Most of the calves collected were infected with BVDV, Pasteurella multocida and mycoplasma. Therefore, in this experiment, the pneumonia of calves infected with Pasteurella multocida, BVDV and mycoplasma was studied.

[0024] Embodiment 1

[0025] Set up Chinese medicine compositions with different proportions, specifically as follows:

[0026] Chinese medicine composition 1: Ephedra 40g, Bupleurum 20g, Codonopsis pilosula 30g, Artemisia annua 20g, Forsythia suspensa 10g, Angelica sinensis 10g, Pueraria lobata 10g.

[0027] Chinese medicine composition 2: Ephedra 30g, Bupleurum 20g, Codonopsis pilosula 20g, Artemisia annua 20g, Forsythia suspensa 10g, Angelica sinensis 10g, Pueraria lobata 10g.

[0028] Chinese medicine composition 3: Ephedra 40g, Bupleurum 30g, Codonopsis pilosula 30g, Artemisia annua 30g, Forsythia suspensa 20g, Angelica sinensis 20g, Pueraria lobata 20g.

[0029] Chinese medicine composition 4: Ephedra 40g, Bupleurum 30g, Codonopsis pilosula 30g, Artemisia annua 20g, Forsythia suspensa 10g, Angelica sinensis 20g, Pueraria lobata 10g.

[0030] After preliminary experiments (inhibitory effect on bovine viral diarrhea virus), it was found that the inhibitory effects of Chinese medicine composition 3 and Chinese medicine composition 4 on bovine viral diarrhea virus were better than those of Chinese medicine composition 1 and Chinese medicine composition 2. Therefore, in subsequent experiments, the prescriptions of Chinese medicine composition 3 and Chinese medicine composition 4 were used for efficacy experiments.

[0031] Comparative Example 1

[0032] Comparative composition 1: 40 g of Ephedra, 30 g of Bupleurum;

[0033] Comparative composition 2: Codonopsis pilosula 30g, Artemisia annua 20g, Forsythia suspensa 10g;

[0034] Comparative composition 3: 20 g of Angelica sinensis and 10 g of Pueraria root;

[0035] Comparative composition 4: 40 g of ephedra, 30 g of bupleurum, 30 g of codonopsis, and 30 g of artemisia annua;

[0036] Experimental Example 1

[0037] 1. Inhibitory effect of Chinese medicine composition on Pasteurella multocida

[0038] Take the isolated Pasteurella multocida for pure culture, inoculate the strain into a suitable culture medium, such as blood agar medium, and culture it at a suitable temperature (about 37°C) and gas environment (such as an environment containing a certain concentration of carbon dioxide) to keep the strain in a good growth state for use.

[0039] Pick a single colony from the cultured Pasteurella multocida plate and inoculate it into a liquid culture medium. Cultivate it under appropriate conditions for a period of time until the bacteria reach the logarithmic growth phase. Then, dilute the bacterial solution appropriately to a concentration of 1*10 5 .

[0040] The minimum inhibitory concentration (MIC) was determined by the test tube double dilution method, and the Chinese medicine composition 3 and the Chinese medicine composition 4, as well as the comparative compositions 1 to 4 were tested respectively. The test was repeated three times, and the results were averaged.

[0041] Table 1 Inhibitory effect of Chinese medicine composition on Streptococcus pneumoniae

[0042]

[0043] Note: * indicates significant difference, P<0.05; ** and *** indicate significant difference, P<0.01

[0044] As shown in Table 1, Chinese medicine composition 3 and Chinese medicine composition 4 can inhibit Streptococcus pneumoniae, which is significantly different from comparative compositions 1-4.

[0045] 2. Inhibitory effect on mycoplasma

[0046] (1) Preparation of Chinese medicine decoction

[0047] Take Chinese medicine composition 3 and Chinese medicine composition 4 in Example 1, add water, and prepare a decoction.

[0048] (2) Mycoplasma strains and culture conditions

[0049] The extracted mycoplasma strains are inoculated into PPLO medium for culture. The pH value of the medium is generally adjusted between 7.6 and 7.8. The culture environment is usually 37°C and contains 5% to 10% CO. 2 The culture should be carried out in a moist environment to meet the growth requirements of mycoplasma. During the culture process, the growth of mycoplasma needs to be observed regularly. Its morphological characteristics can be observed under a microscope, and its growth curve can be monitored using methods such as turbidimetry to ensure that mycoplasma is in a good growth state.

[0050] When the mycoplasma grows to the logarithmic growth phase, collect the bacterial solution. Separate the mycoplasma cells from the culture medium by centrifugation or other methods, then resuspend the mycoplasma cells in fresh culture medium and adjust the concentration of the bacterial solution to an appropriate range. Generally, it can be diluted to a certain colony forming unit (CFU) or optical density value (OD value) according to the experimental requirements for use in drug efficacy experiments.

[0051] The Chinese medicine composition 3, the Chinese medicine composition 4, and the comparative compositions 1-4 were used to carry out the anti-Mycoplasma pneumoniae pharmacodynamic test, and the in vitro anti-Mycoplasma pneumoniae MIC value was determined by the two-fold serial dilution method of 96-well cell culture plates, and the MBC value was determined by centrifuge tubes.

[0052] Table 2 Detection of the efficacy of Chinese medicine composition on mycoplasma

[0053]

[0054] Note: * indicates significant difference, P<0.05; ** and *** indicate significant difference, P<0.01

[0055] As shown in Table 2, Chinese medicine composition 3 and Chinese medicine composition 4 can inhibit the growth of mycoplasma, which is significantly different from that of comparative compositions 1-4.

[0056] Experimental Example 2: Animal Experiment

[0057] (1) Methods

[0058] Calves with symptoms such as fever, cough, dyspnea, depression and loss of appetite were selected from farms in Karamay area and preliminarily diagnosed as calf pneumonia. After laboratory pathogen testing, calves with mixed infections of Streptococcus pneumoniae, bovine viral diarrhea virus and mycoplasma were screened out for experiments.

[0059] A total of 120 mixed-infected calves were screened and divided into 6 groups, with 20 calves in each group.

[0060] The decoctions of Chinese medicine composition 3, Chinese medicine composition 4 and comparative compositions 1-4 were administered intragastrically three times a day, 200-300 ml each time. After continuous administration for 5 days, the symptoms were observed and the pathogens were detected.

[0061] (2) Efficacy evaluation criteria:

[0062] Cure: Clinical symptoms and signs such as fever, cough, dyspnea, depression and loss of appetite completely disappeared, and functional activities returned to normal.

[0063] Effective: Clinical symptoms such as fever, cough, dyspnea, depression and loss of appetite are significantly improved or enhanced.

[0064] Ineffective: There was no significant improvement in clinical symptoms and signs.

[0065] (3) Results

[0066] Table 3 The therapeutic effect of Chinese medicine composition on calf pneumonia

[0067]

[0068]

[0069] Note: * indicates significant difference, P<0.05; ** and *** indicate significant difference, P<0.01

[0070] From the results in Table 3, it can be seen that Chinese medicine composition 3 and Chinese medicine composition 4 can be used to treat mixed pneumonia in calves. Chinese medicine composition 3 treated 20 calves, of which 17 were cured and the symptoms completely disappeared, and the symptoms of 3 were significantly alleviated, and the frequency of coughing was significantly reduced. The cure rate was 85%, and the effective rate was 100%; Chinese medicine composition 4 treated 20 calves, of which 15 were cured and the symptoms completely disappeared, and the symptoms of 5 were significantly alleviated, without fever, increased appetite, and although there was cough, the frequency was significantly reduced. The cure rate was 75%, and the effective rate was 100%; however, compared with the Chinese medicine compositions described in compositions 1-3, the therapeutic effect was very poor, and due to individual differences, it was only effective for a few calves.

[0071] In summary, the present invention provides a traditional Chinese medicine composition for preventing and treating mixed pneumonia in calves. The traditional Chinese medicine composition can treat mixed pneumonia in calves, and the cure rate is as high as 100%. At the same time, the traditional Chinese medicine composition has small toxic and side effects, low cost, and is not easy to produce drug resistance, so it has broad application prospects.

[0072] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating mixed pneumonia in calves, characterized in that: The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 30-40 parts of ephedra, 20-30 parts of bupleurum, 20-30 parts of codonopsis, 20-30 parts of artemisia annua, 10-20 parts of forsythia, 10-20 parts of angelica and 10-20 parts of kudzu root.

2. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30-40 parts of ephedra, 20-30 parts of bupleurum, 20-30 parts of codonopsis, 20-30 parts of artemisia annua, 10-20 parts of forsythia, 10-20 parts of angelica and 10-20 parts of kudzu root.

3. The Chinese medicine composition according to claim 2, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 20 parts of bupleurum, 30 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 10 parts of angelica and 10 parts of kudzu root.

4. The Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of ephedra, 20 parts of bupleurum, 20 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 10 parts of angelica and 10 parts of kudzu root.

5. The Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 30 parts of bupleurum, 30 parts of codonopsis, 30 parts of artemisia annua, 20 parts of forsythia, 20 parts of angelica and 20 parts of kudzu root.

6. The Chinese medicine composition according to claim 2, characterized in that: The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of ephedra, 30 parts of bupleurum, 30 parts of codonopsis, 20 parts of artemisia annua, 10 parts of forsythia, 20 parts of angelica and 10 parts of kudzu root.

7. Use of the Chinese medicine composition as described in any one of claims 1 to 6 in the preparation of a medicine for preventing and treating mixed pneumonia in calves.

8. Use of the Chinese medicine composition according to any one of claims 1 to 6 in the preparation of a feed additive for preventing and treating mixed pneumonia in calves.

9. A feed additive prepared by the Chinese medicine composition according to any one of claims 1 to 6.

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