A closed mixotrophic culture method and culture system for microalgae

Abstract

The invention discloses a closed mixotrophic microalgae culture method and a culture system thereof, and relates to the field of microalgae culture, comprising the following steps: step one, microalgae culture, pouring culture solution into a culture tank, adding microalgae to be cultured into the culture solution, and then fixing a sealing plate to the tank mouth of the culture tank by bolts to carry out closed culture; step two, gas supply and nutrient solution addition, sending carbon dioxide from a pipeline into an air distribution pipe through an air pump in an air supply component, forming bubbles from a nozzle and transporting them into the culture solution, and part of the bubbles that rise are gathered by a baffle to extend their residence time in a water body; step three, illumination, during the day, a first electric telescopic rod drives an arc-shaped telescopic plate to move downward to expose the internal transparent culture tank, and sunlight provides a light source to propagate the microalgae. The application can extend the residence time of carbon dioxide in the water body, improve the utilization rate of carbon dioxide, and increase the yield of microalgae.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of microalgae cultivation, and specifically relates to a closed mixotrophic cultivation method of microalgae and a cultivation system thereof. Background Art

[0002] As a single-cell organism, microalgae grows much faster than terrestrial plants, so they have a stronger photosynthetic carbon fixation capacity. There are two main ways to cultivate microalgae on a large scale: open microalgae cultivation system and closed microalgae cultivation system. The open cultivation system is to cultivate microalgae outdoors using natural sunlight, which has the advantages of being easier to expand and lower cost. The closed microalgae cultivation system refers to the cultivation of microalgae in a closed container using culture medium.

[0003] A microalgae aseptic culture device and culture method suitable for mixotrophic culture described in the prior art include: a culture tank cover, a tank body, a base, and a light source. The upper and lower bottom surfaces of the tank body are both open. The culture tank cover is detachably arranged at the opening of the upper bottom surface of the tank body. The lower bottom surface of the tank body is detachably connected to the base. The bottom of the base is a funnel-shaped structure, and a discharge port is provided at the lower end of the funnel-shaped structure. The light source is arranged inside the tank body and connected to the culture tank cover. The tank body is composed of a reflective outer wall and a transparent inner wall. An air distribution structure is laid in the base, and the air distribution structure is composed of two strip air distribution plates connected in series, one of which is connected to the air inlet.

[0004] Although the above technology can maximize the efficiency of light energy utilization and improve the photosynthesis efficiency of microalgae, thus achieving the purpose of saving costs and increasing yields, during the cultivation process, when carbon dioxide is injected through the gas distribution structure, the carbon dioxide will stay in the water for a short time, resulting in a low utilization rate. Summary of the invention

[0005] Based on this, the object of the present invention is to provide a closed mixotrophic culture method and a culture system for microalgae to solve the technical problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A closed mixotrophic culture method for microalgae comprises the following steps:

[0008] Step 1: Cultivating microalgae: pouring the culture solution into a culture tank, adding the microalgae to be cultivated into the culture solution, and then fixing the sealing plate to the tank mouth of the culture tank by bolts for closed cultivation;

[0009] Step 2: supply air and add nutrient solution. The carbon dioxide is sent into the air distribution pipe through the air pump in the air supply assembly. Bubbles are formed from the nozzle and transported to the culture solution. The rising part of the bubbles is gathered by the baffle to extend the residence time in the water body. At the same time, the nutrient solution is added to the culture tank through the addition tube on the sealing plate.

[0010] Step 3: Illumination: During the day, the first electric telescopic rod drives the arc-shaped telescopic plate to move downward, exposing the transparent culture tank inside, and the sunlight provides light source for propagating the microalgae. After the arc-shaped telescopic plate is retracted to the limit, it touches the sensor head, and the two components in the cleaning mechanism are activated to slowly scrape the inner wall of the culture tank and the outer wall of the light rod, and at the same time slowly stir the culture solution and the microalgae;

[0011] At night, the arc-shaped telescopic plate unfolds to cover the culture tank inside, and the light sticks are activated to provide additional light for the microalgae.

[0012] The technical solution is specific, the bottom of the culture tank is installed with a placement plate by screws, the upper surface of the placement plate is located on one side of the culture tank and a vertical side support plate is fixed thereto, the arc-shaped telescopic plate is fixed to the lower surface of the placement plate by screws, the telescopic end of the arc-shaped telescopic plate passes through the placement plate and contacts with the lower surface of the sealing plate, and the arc groove of the telescopic end of the arc-shaped telescopic plate contacts with the side wall of the side support plate, a light stick is vertically installed at the center of the lower surface of the sealing plate, an air supply assembly is provided on the inner bottom wall of the culture tank, an air pump provided in the air supply assembly is installed on one side of the lower surface of the placement plate by screws, a protective shell is installed on the top of the sealing plate by screws, a cleaning mechanism is provided in the protective shell, and the cleaning mechanism consists of a rotating assembly and a vertical movement assembly.

[0013] Specifically, the output port of the vacuum pump is connected to a branch pipe, the top end of the branch pipe passes through the placement plate and the culture tank and is connected to a horizontal air supply pipe, both ends of the air supply pipe are connected to both ends of the air distribution pipe, a plurality of nozzles are provided on the upper surface of the air distribution pipe, three mounting blocks are evenly provided below the air distribution pipe, three baffles are evenly provided above the air distribution pipe, each of the baffles is connected to the mounting block through two fixing rods, and the two fixing rods are symmetrically arranged on both sides of the air distribution pipe.

[0014] Specifically, each of the baffles is composed of two integral inclined plates with an angle of 130°, the lower surface of each mounting block is fixedly connected to the culture tank by screws, and the two ends of each fixing rod are respectively fixedly connected to the lower surface of the baffle and the upper surface of the mounting block.

[0015] Specifically, the rotating assembly includes four rubber scrapers in contact with the inner wall of the culture tank, each of the rubber scrapers is integrally provided with a stirring plate on a side away from the inner wall of the culture tank, a ring plate is sleeved on the lamp stick, the outer wall of the ring plate is fixedly connected to the rubber scraper by four connecting rods, a driving motor is installed on one side of the lower surface of the protective shell by screws, a driving gear is fixedly sleeved on the output end of the driving motor, a gear ring is provided above the sealing plate, and the driving gear is meshed with the tooth surface of the gear ring.

[0016] Specifically, vertical support rods are fixed on the upper surfaces of the two connecting rods, and first arc-shaped through grooves are symmetrically opened on the sealing plate, and the moving angle of each of the first arc-shaped through grooves is 90°. The top end of each support rod passes through the first arc-shaped through groove and is fixedly connected to the lower surface of the gear ring, and shielding arc plates are slidably provided on the sealing plate at the two first arc-shaped through grooves, and one end of each shielding arc plate is fixedly connected to the outer wall of the support rod.

[0017] Specifically, the technical solution of the present invention is that the vertical movement assembly includes a circular plate, the lower surface of the circular plate is in contact with the upper surface of the sealing plate and is slidably connected, and a second arc-shaped through groove is symmetrically opened on the sealing plate below the circular plate, and the moving angle of each of the second arc-shaped through grooves is 90°. The upper surface of the circular plate is symmetrically installed with second electric telescopic rods, and the telescopic ends of the two second electric telescopic rods successively pass through the circular plate, the second arc-shaped through groove and the ring plate arranged in the rotating assembly, and an annular plate is provided below the ring plate, and the telescopic ends of the two second electric telescopic rods are fixedly connected to the annular plate by screws, and the inner ring wall of the annular plate is provided with a wiping cotton and is in contact with the outer wall of the lamp stick.

[0018] Specifically, support legs are installed on both sides of the lower surface of the placement plate by screws, a first electric telescopic rod is installed on the side of the lower surface of the placement plate away from the side support plate by screws, the telescopic end of the first electric telescopic rod passes through the placement plate and is screwed to a fixing plate, the fixing plate is fixedly connected to the top of the outer wall of the telescopic end of the arc-shaped telescopic plate, a sensor is embedded in one side of the lower surface of the fixing plate, a sensor head is embedded in one side of the upper surface of the placement plate, the sensor head cooperates with the sensor, and a controller is installed on one side of the inner top wall of the protective shell.

[0019] According to the above technical scheme, a cultivation system for a closed mixotrophic cultivation method of microalgae will also be provided, wherein the controller is provided with a main control module, a detection module and a wireless connection module, wherein the detection module is used to detect various data and send the detection data to the main control module, so as to facilitate control of the cultivation device, wherein the main control module is used to receive signals sent by the sensor and the detection module, and to control the first electric telescopic rod, the vacuum pump, the drive motor and the second electric telescopic rod according to the signal content, and wherein the wireless connection module is used to connect to the user's terminal via a wireless network, so as to facilitate implementation of understanding of the cultivation situation.

[0020] Specifically, the detection module includes an environment detection unit, a carbon dioxide detection unit and a light detection unit. The environment detection unit is used to sense sunlight and judge that it is daytime when a set interval value is reached. The carbon dioxide detection unit is used to detect the carbon dioxide content in the culture tank. The light detection unit is used to detect the light intensity in the culture tank.

[0021] In summary, the present invention mainly has the following beneficial effects: the present application uses an air pump to transport carbon dioxide into the air distribution pipe and sprays it out from a number of nozzles, wherein some carbon dioxide bubbles are clustered by the baffle plate above, prolonging the residence time in the water body, improving the utilization rate of carbon dioxide, and increasing the yield of microalgae. When the environmental detection unit senses that the sunlight reaches the set interval value, a signal is sent to the main control module, the first electric telescopic rod is contracted, and the moving fixed plate drives the telescopic end of the arc-shaped telescopic plate to be folded and stored, exposing the transparent culture tank, and providing a light source for the microalgae in the tank through sunlight;

[0022] After the sensor on the fixed plate contacts the sensor head, the sensor head sends a signal to the main control module, starts the drive motor in the rotating assembly, and drives the four rubber scrapers to move slowly back and forth along the inner wall of the culture tank through the gear assembly to scrape off the microalgae attached to the inner wall of the culture tank. When the environmental detection unit detects that the sunlight is lower than or higher than the set interval value, the arc-shaped telescopic plate is controlled to unfold to avoid the temperature being too low or too high. Then the light stick is started to supplement the light for the microalgae. At this time, the light detection unit detects the light intensity in the tank. When the detection value is lower than the set value, the main control module starts the vertical movement assembly and scrapes off the microalgae attached to the outer wall of the light stick through the moving annular plate, thereby ensuring light transmittance and stirring the microalgae to make the light uniform and improve the light utilization efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 is a flow chart of the method of the present invention;

[0024] Figure 2 is a schematic diagram of a culture device of the present invention;

[0025] Figure 3 This is a structural diagram of the arc-shaped telescopic plate of the present invention;

[0026] Figure 4 It is a schematic diagram of the internal structure of the tank of the present invention;

[0027] Figure 5 It is a structural diagram of the gas supply assembly of the present invention;

[0028] Figure 6 It is a schematic diagram of the cleaning mechanism of the present invention;

[0029] Figure 7 It is a schematic diagram of a sealing plate of the present invention;

[0030] Figure 8 It is a system block diagram of the present invention.

[0031] Description of the drawings: 1. Placement plate; 101. Support leg; 102. Side bearing plate; 103. Sensor head; 2. Arc-shaped telescopic plate; 201. Fixing plate; 202. First electric telescopic rod; 3. Culture tank; 301. Sealing plate; 3011. First arc-shaped through groove; 3012. Second arc-shaped through groove; 302. Protective shell; 303. Light stick; 4. Air supply assembly; 401. Air pump; 402. Air supply pipe; 403. Branch pipe; 404. Air distribution pipe; 405. 041, nozzle; 405, mounting block; 406, baffle; 4061, fixing rod; 5, rotating assembly; 501, rubber scraper; 5011, stirring plate; 502, connecting rod; 503, collar plate; 504, supporting rod; 5041, shielding arc plate; 505, gear ring; 506, driving gear; 507, driving motor; 6, vertical moving assembly; 601, circular plate; 602, second electric telescopic rod; 603, annular plate; 7, cleaning mechanism. DETAILED DESCRIPTION

[0032] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0033] The following describes an embodiment of the present invention based on its overall structure.

[0034] Example

[0035] See also Figure 1-4 As shown, a closed mixotrophic culture method for microalgae comprises the following steps:

[0036] Step 1: Cultivate microalgae. Pour the culture solution into the culture tank 3, add the microalgae to be cultivated into the culture solution, and then fix the sealing plate 301 to the tank mouth of the culture tank 3 by bolts to perform closed cultivation.

[0037] Step 2: supplying air and adding nutrient solution. The air pump 401 in the air supply assembly 4 delivers carbon dioxide from the pipeline into the air distribution pipe 404, and bubbles are formed from the nozzle 4041 and transported to the culture solution. The rising part of the bubbles is gathered by the baffle 406 to extend the residence time in the water body. At the same time, the nutrient solution is added to the culture tank 3 through the addition pipe on the sealing plate 301.

[0038] Step 3: Illumination: During the day, the first electric telescopic rod 202 drives the arc-shaped telescopic plate 2 to move downward, exposing the transparent culture tank 3 inside, and using sunlight as a light source to propagate the microalgae. After the arc-shaped telescopic plate 2 is retracted to the limit, it touches the sensor head 103, and the two components in the cleaning mechanism 7 are activated to slowly scrape the inner wall of the culture tank 3 and the outer wall of the light rod 303, respectively, and slowly stir the culture solution and the microalgae;

[0039] At night, the arc-shaped telescopic plate 2 is unfolded to cover the culture tank 3 inside, and the light stick 303 is activated to provide additional light for the microalgae;

[0040] The sealing plate 301 in the present application is also provided with an air outlet pipe and a feed pipe, wherein the air outlet pipe is provided with a microporous filter for discharging the gas filled in the culture tank 3 and filtering and removing bacteria through the microporous filter to prevent external bacteria from entering the tank body and contaminating the microalgae in the tank body;

[0041] The air pump 401 is connected to a carbon dioxide gas source through a pipeline, and a discharge pipe is provided at the center of the bottom of the culture tank 3, and the discharge pipe penetrates the placement plate 1 and is located at the center of the arc-shaped telescopic plate 2;

[0042] A placement plate 1 is installed at the bottom of the culture tank 3 by screws, and a vertical side bearing plate 102 is fixed to the upper surface of the placement plate 1 located on one side of the culture tank 3, and an arc-shaped telescopic plate 2 is fixed to the lower surface of the placement plate 1 by screws, and the telescopic end of the arc-shaped telescopic plate 2 passes through the placement plate 1 and contacts the lower surface of the sealing plate 301, and the arc groove of the telescopic end of the arc-shaped telescopic plate 2 contacts the side wall of the side bearing plate 102, and a light stick 303 is vertically installed at the center of the lower surface of the sealing plate 301, and an air supply component 4 is provided on the inner bottom wall of the culture tank 3, and an air pump 401 provided in the air supply component 4 is installed on one side of the lower surface of the placement plate 1 by screws, and a protective shell 302 is installed on the top of the sealing plate 301 by screws, and a cleaning mechanism 7 is provided in the protective shell 302, and the cleaning mechanism 7 is composed of a rotating component 5 and a vertical moving component 6;

[0043] The output port of the vacuum pump 401 is connected to a branch pipe 403, the top of the branch pipe 403 passes through the placement plate 1 and the culture tank 3 and is connected to a horizontal air supply pipe 402, both ends of the air supply pipe 402 are connected to both ends of the air distribution pipe 404, a plurality of nozzles 4041 are provided on the upper surface of the air distribution pipe 404, three mounting blocks 405 are evenly provided below the air distribution pipe 404, three baffles 406 are evenly provided above the air distribution pipe 404, each baffle 406 is connected to the mounting block 405 through two fixing rods 4061, the two fixing rods 4061 are symmetrically arranged on both sides of the air distribution pipe 404, each baffle 406 is composed of two integral inclined plates with an angle of 130°, the lower surface of each mounting block 405 is fixedly connected to the culture tank 3 by screws, and the two ends of each fixing rod 4061 are respectively fixedly connected to the lower surface of the baffle 406 and the upper surface of the mounting block 405;

[0044] Support legs 101 are installed on both sides of the lower surface of the placement plate 1 by screws, and a first electric telescopic rod 202 is installed on the side of the lower surface of the placement plate 1 away from the side support plate 102 by screws. The telescopic end of the first electric telescopic rod 202 passes through the placement plate 1 and is screwed with a fixed plate 201. The fixed plate 201 is fixedly connected to the top of the outer wall of the telescopic end of the arc-shaped telescopic plate 2. A sensor is embedded in one side of the lower surface of the fixed plate 201, and a sensor head 103 is embedded in one side of the upper surface of the placement plate 1. The sensor head 103 cooperates with the sensor, and a controller is installed on one side of the inner top wall of the protective shell 302.

[0045] When the microalgae are cultured in a closed mixotrophic manner, the operator passes the culture solution into the culture tank 3, and adds the microalgae to be cultured into the culture solution, and then fixes the sealing plate 301 to the tank mouth of the culture tank 3 by bolts for closed culture, and then adds nutrient solution and biological inhibitors (such as calcium sulfate can inhibit the reproduction of rotifers, and medical concentrated ammonia can effectively kill sharp-nosed worms) into the culture tank 3 through the feed pipe, and uses the carbon dioxide detection unit to detect the carbon dioxide content in the water body in real time. When it is lower than the set value, the carbon dioxide detection unit is used ... The signal main control module starts the air pump 401 in the air supply component 4. The air pump 401 delivers the carbon dioxide in the carbon dioxide supply source into the air supply pipe 402 through the branch pipe 403, and delivers the two ends of the air supply pipe 402 into the air distribution pipe 404, and then sprays out from a plurality of nozzles 4041 to form small bubbles. During the process of the bubbles floating up, some bubbles will be intercepted by the baffle 406 above and gathered under the action of the angle, thereby prolonging the residence time in the water body, thereby improving the utilization rate of carbon dioxide and increasing the yield of microalgae;

[0046] When the environment detection unit senses that the sunlight reaches the set interval value, it sends a signal to the main control module, and the first electric telescopic rod 202 contracts, driving the fixed plate 201 to move downward, and the fixed plate 201 drives the telescopic end of the arc-shaped telescopic plate 2 to move downward, so that the arc-shaped telescopic plate 2 is folded and stored, exposing the transparent culture tank 3, and providing light source for the microalgae in the tank through sunlight. After the fixed plate 201 moves down to the placement plate 1, the sensor embedded in its lower surface will contact the sensor head 103. At this time, the sensor head 103 sends a signal to the main control module to start the rotating component 5, so that the actuator of the rotating component 5 (the rubber scraper 501 in the text) moves slowly back and forth along the inner wall of the culture tank 3. During the movement, the microalgae attached to the inner wall are scraped off, thereby ensuring the light transmittance of the tank wall, and stirring the microalgae to make the light uniform and improve the light utilization efficiency. During the stirring process, the carbon dioxide bubbles gathered by the baffle 406 are driven to move, further increasing the dissolution of carbon dioxide and water.

[0047] Then, when the environment detection unit detects that the sunlight is lower than or higher than the set interval value, the first electric telescopic rod 202 works to control the arc-shaped telescopic plate 2 to expand to avoid the temperature being too low or too high. Then, the main control module starts the lamp stick 303 to supplement the light for the microalgae. At this time, the light detection unit detects the light intensity in the tank. When the detection value is lower than the set value, the main control module starts the vertical movement component 6 to scrape off the microalgae attached to the outer wall of the lamp stick 303, thereby further ensuring the light utilization effect.

[0048] See also Figure 4 , Figure 6 and Figure 7 As shown, the rotating assembly 5 includes four rubber scrapers 501 in contact with the inner wall of the culture tank 3, and each rubber scraper 501 is integrally provided with a stirring plate 5011 on a side away from the inner wall of the culture tank 3. A collar plate 503 is sleeved on the lamp stick 303, and the outer wall of the collar plate 503 is fixedly connected to the rubber scraper 501 through four connecting rods 502. A driving motor 507 is installed on one side of the lower surface of the protective shell 302 by screws, and a driving gear 506 is fixedly sleeved on the output end of the driving motor 507. A gear ring 505 is provided above the sealing plate 301, and the driving gear 50 6 is meshed with the tooth surface of the gear ring 505, a vertical support rod 504 is fixed on the upper surface of the two connecting rods 502, and the sealing plate 301 is symmetrically provided with first arc-shaped through grooves 3011, and the movement angle of each first arc-shaped through groove 3011 is 90°. The top of each support rod 504 passes through the first arc-shaped through groove 3011 and is fixedly connected to the lower surface of the gear ring 505. The sealing plate 301 is slidably provided with shielding arc plates 5041 at the two first arc-shaped through grooves 3011, and one end of each shielding arc plate 5041 is fixedly connected to the outer wall of the support rod 504;

[0049] The vertical movement component 6 includes a circular plate 601, the lower surface of the circular plate 601 is in contact with the upper surface of the sealing plate 301 and is slidably connected, and a second arc-shaped through groove 3012 is symmetrically opened on the sealing plate 301 below the circular plate 601, and the movement angle of each second arc-shaped through groove 3012 is 90°. The second electric telescopic rod 602 is symmetrically installed on the upper surface of the circular plate 601, and the telescopic ends of the two second electric telescopic rods 602 pass through the circular plate 601, the second arc-shaped through groove 3012 and the ring plate 503 provided in the rotating component 5 in sequence. A ring plate 603 is provided below the ring plate 503, and the telescopic ends of the two second electric telescopic rods 602 are fixedly connected to the ring plate 603 by screws, and the inner ring wall of the ring plate 603 is provided with a wiping cotton and contacts the outer wall of the lamp stick 303.

[0050] When the rotating assembly 5 is running, the driving motor 507 works, and drives the driving gear 506 to rotate back and forth through the output end. The tooth surface of the driving gear 506 drives the meshing gear ring 505 to rotate back and forth. The rotation of the gear ring 505 drives the support rod 504 to move along the first arc-shaped through groove 3011. The moving support rod 504 drives the connecting rod 502 to rotate back and forth, and the connecting rod 502 drives the collar plate 503 and the rubber scraper 501 to move. The collar plate 503 moves along the lamp stick 303. The outer wall rotates, and the circular plate 601 and the annular plate 603 are driven to rotate along the second arc-shaped groove 3012 by the second electric telescopic rod 602, wherein under the shielding of the shielding arc plate 5041 and the circular plate 601, water vapor can be prevented from entering the protective shell 302 through the first arc-shaped groove 3011 and the second arc-shaped groove 3012, and the movement of the rubber scraper 501 will scrape off the microalgae attached to the inner wall of the culture tank 3, and at the same time, the rubber scraper 501 will also drive the stirring plate 5011 to stir the water body;

[0051] When the vertical moving assembly 6 is in operation, the second electric telescopic rod 602 works, and its telescopic end moves downward along the outer wall of the lamp stick 303 through the annular plate 603, and the microalgae attached to the outer wall of the lamp stick 303 are scraped off by the wiping cotton.

[0052] See also Figure 1-8 As shown, according to the above embodiment, a culture system for a closed mixotrophic culture method of microalgae is also provided. A main control module, a detection module and a wireless connection module are provided in the controller. The detection module is used to detect various data and send the detection data to the main control module, so as to control the culture device. The main control module is used to receive signals sent by the sensor and the detection module, and control the first electric telescopic rod 202, the vacuum pump 401, the drive motor 507 and the second electric telescopic rod 602 according to the signal content. The wireless connection module is used to connect to the user's terminal through a wireless network to facilitate the implementation of understanding the culture situation.

[0053] The detection module includes an environment detection unit, a carbon dioxide detection unit and a light detection unit. The environment detection unit is used to sense sunlight and judge that it is daytime when the set interval value is reached. The carbon dioxide detection unit is used to detect the carbon dioxide content in the culture tank 3. The light detection unit is used to detect the light intensity in the culture tank 3.

[0054] The working principle of the present invention is:

[0055] When the microalgae are cultured in a closed and mixed culture, the operator passes the culture solution into the culture tank 3, and adds the microalgae to be cultured into the culture solution, and then fixes the sealing plate 301 to the tank mouth of the culture tank 3 by bolts for closed culture, and then adds nutrient solution and biological inhibitors into the culture tank 3 through the feed pipe, and uses the carbon dioxide detection unit to detect the carbon dioxide content in the water body in real time. When it is lower than the set value, the main control module sends a signal to start the air pump 401 in the air supply component 4, and the air pump 401 delivers the carbon dioxide in the carbon dioxide supply source into the air supply pipe 402 through the branch pipe 403, and sends the two ends of the air supply pipe 402 into the air distribution pipe 404, and then sprays out from a number of nozzles 4041 to form small bubbles. In the process of the bubbles floating up, some bubbles will be intercepted by the baffle 406 above, and gathered under the action of the angle, thereby prolonging the residence time in the water body.

[0056] When the environment detection unit senses that the sunlight reaches the set interval value, it sends a signal to the main control module, and the first electric telescopic rod 202 contracts, driving the fixed plate 201 to move downward, and the fixed plate 201 drives the telescopic end of the arc-shaped telescopic plate 2 to move downward, so that the arc-shaped telescopic plate 2 is folded and stored, exposing the transparent culture tank 3, and providing light source for the microalgae in the tank through sunlight. After the fixed plate 201 moves down to the placement plate 1, the sensor embedded in its lower surface will contact the sensor head 103. At this time, the sensor head 103 sends a signal to the main control module to start the drive motor 507, and drives the drive gear 506 to rotate reciprocatingly through the output end. The tooth surface of the drive gear 506 drives the meshing gear ring 505 to rotate reciprocatingly, and the rotation of the gear ring 505 drives the support rod 504 to move along the first arc-shaped through groove 3011, and the moving support rod 504 drives the connecting rod 504 to move. The rod 502 performs a reciprocating angle rotation, and the connecting rod 502 drives the collar plate 503 and the rubber scraper 501 to move. The collar plate 503 rotates on the outer wall of the lamp stick 303, and drives the circular plate 601 and the annular plate 603 to rotate along the second arc-shaped groove 3012 through the second electric telescopic rod 602. Under the shielding of the shielding arc plate 5041 and the circular plate 601, water vapor can be prevented from entering the protective shell 302 through the first arc-shaped groove 3011 and the second arc-shaped groove 3012. The movement of the rubber scraper 501 will scrape off the microalgae attached to the inner wall of the culture tank 3. At the same time, the rubber scraper 501 will also drive the stirring plate 5011 to stir the water body, so that the light is uniform and the light utilization efficiency is improved. In the stirring process, the carbon dioxide bubbles gathered by the baffle 406 are driven to move, and the dissolution of carbon dioxide and water is further increased.

[0057] Then, when the environment detection unit detects that the sunlight is lower than or higher than the set interval value, the first electric telescopic rod 202 works to control the arc-shaped telescopic plate 2 to expand to avoid the temperature being too low or too high. Then, the lamp stick 303 is started through the main control module to supplement the light for the microalgae. At this time, the light detection unit detects the light intensity in the tank. When the detected value is lower than the set value, the main control module starts the second electric telescopic rod 602, and its telescopic end will move down along the outer wall of the lamp stick 303 through the annular plate 603, and the microalgae attached to the outer wall of the lamp stick 303 will be scraped off by the wiping cotton to further ensure the light utilization effect.

[0058] Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the invention. The specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions and variations to the embodiments without creative contributions as needed without departing from the principles and purpose of the present invention. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A closed mixotrophic culture method for microalgae, characterized in that , including the following steps: Step 1: Cultivate microalgae. Pour the culture solution into the culture tank (3), add the microalgae to be cultivated into the culture solution, and then fix the sealing plate (301) to the tank mouth of the culture tank (3) by bolts to perform closed cultivation. Step 2: supplying air and adding nutrient solution. The carbon dioxide is sent from the pipeline into the air distribution pipe (404) through the air pump (401) in the air supply component (4). Bubbles are formed from the nozzle (4041) and transported to the culture solution. The rising part of the bubbles is gathered by the baffle (406) to extend the residence time in the water body. At the same time, the nutrient solution is added to the culture tank (3) through the addition pipe on the sealing plate (301); Step 3: illumination. During the day, the first electric telescopic rod (202) drives the arc-shaped telescopic plate (2) to move downward, exposing the transparent culture tank (3) inside, and using sunlight as a light source to propagate the microalgae. After the arc-shaped telescopic plate (2) is retracted to the limit, it touches the sensor head (103), and the two components in the cleaning mechanism (7) are activated to respectively perform a slow scraping operation on the inner wall of the culture tank (3) and the outer wall of the light rod (303), and at the same time, the culture solution and the microalgae are slowly stirred. At night, the arc-shaped telescopic plate (2) is unfolded to cover the culture tank (3) inside, and the light stick (303) is activated to provide additional light for the microalgae.

2. A closed mixotrophic culture method for microalgae according to claim 1, characterized in that: A placement plate (1) is installed at the bottom of the culture tank (3) by means of screws. A vertical side support plate (102) is fixed to the upper surface of the placement plate (1) on one side of the culture tank (3). The arc-shaped telescopic plate (2) is fixed to the lower surface of the placement plate (1) by means of screws. The telescopic end of the arc-shaped telescopic plate (2) penetrates the placement plate (1) and contacts the lower surface of the sealing plate (301). The arc groove of the telescopic end of the arc-shaped telescopic plate (2) contacts the side wall of the side support plate (102). A light stick (303) is vertically installed at the center of the lower surface of (301), and an air supply component (4) is provided on the inner bottom wall of the culture tank (3). The air pump (401) provided in the air supply component (4) is installed on one side of the lower surface of the placement plate (1) by screws. A protective shell (302) is installed on the top of the sealing plate (301) by screws. A cleaning mechanism (7) is provided in the protective shell (302), and the cleaning mechanism (7) is composed of a rotating component (5) and a vertical moving component (6).

3. A closed mixotrophic culture method for microalgae according to claim 2, characterized in that: The output port of the vacuum pump (401) is connected to a branch pipe (403), the top end of the branch pipe (403) passes through the placement plate (1) and the culture tank (3) and is connected to a horizontal air supply pipe (402), the two ends of the air supply pipe (402) are connected to the two ends of the air distribution pipe (404), a plurality of nozzles (4041) are provided on the upper surface of the air distribution pipe (404), three mounting blocks (405) are evenly arranged below the air distribution pipe (404), and three baffles (406) are evenly arranged above the air distribution pipe (404), each of the baffles (406) is connected to the mounting block (405) by two fixing rods (4061), and the two fixing rods (4061) are symmetrically arranged on both sides of the air distribution pipe (404).

4. A closed mixotrophic culture method for microalgae according to claim 3, characterized in that: Each of the baffles (406) is composed of two integral inclined plates with an included angle of 130°. The lower surface of each mounting block (405) is fixedly connected to the culture tank (3) by screws, and the two ends of each fixing rod (4061) are respectively fixedly connected to the lower surface of the baffle (406) and the upper surface of the mounting block (405).

5. The closed mixotrophic culture method of microalgae according to claim 2, characterized in that: The rotating assembly (5) comprises four rubber scrapers (501) in contact with the inner wall of the culture tank (3); a stirring plate (5011) is integrally provided on a side of each rubber scraper (501) away from the inner wall of the culture tank (3); a collar plate (503) is sleeved on the lamp stick (303); the outer wall of the collar plate (503) is fixedly connected to the rubber scraper (501) via four connecting rods (502); a driving motor (507) is mounted on one side of the lower surface of the protective shell (302) via screws; a driving gear (506) is fixedly sleeved on the output end of the driving motor (507); a gear ring (505) is provided above the sealing plate (301); the driving gear (506) is meshedly connected to the tooth surface of the gear ring (505).

6. A closed mixotrophic culture method for microalgae according to claim 5, characterized in that: A vertical support rod (504) is fixed on the upper surface of the two connecting rods (502); first arc-shaped through grooves (3011) are symmetrically provided on the sealing plate (301); the movement angle of each of the first arc-shaped through grooves (3011) is 90°; the top end of each of the support rods (504) passes through the first arc-shaped through groove (3011) and is fixedly connected to the lower surface of the gear ring (505); shielding arc plates (5041) are slidably provided at the two first arc-shaped through grooves (3011) on the sealing plate (301); one end of each of the shielding arc plates (5041) is fixedly connected to the outer wall of the support rod (504).

7. The closed mixotrophic culture method of microalgae according to claim 2, characterized in that: The vertical movement assembly (6) comprises a circular plate (601), the lower surface of the circular plate (601) contacts and is slidably connected to the upper surface of the sealing plate (301), the sealing plate (301) is symmetrically provided with a second arc-shaped through groove (3012) below the circular plate (601), the movement angle of each of the second arc-shaped through grooves (3012) is 90°, the upper surface of the circular plate (601) is symmetrically provided with second electric telescopic rods (602), the telescopic ends of the two second electric telescopic rods (602) pass through the circular plate (601), the second arc-shaped through groove (3012) and the collar plate (503) provided in the rotating assembly (5) in sequence, an annular plate (603) is provided below the collar plate (503), the telescopic ends of the two second electric telescopic rods (602) are both fixedly connected to the annular plate (603) by screws, the inner ring wall of the annular plate (603) is provided with a wiping sponge and contacts the outer wall of the lamp stick (303).

8. The closed mixotrophic culture method of microalgae according to claim 2, characterized in that: Support legs (101) are installed on both sides of the lower surface of the placement plate (1) by means of screws. A first electric telescopic rod (202) is installed on the side of the lower surface of the placement plate (1) away from the side support plate (102) by means of screws. The telescopic end of the first electric telescopic rod (202) passes through the placement plate (1) and is screwed with a fixing plate (201). The fixing plate (201) is fixedly connected to the top of the outer wall of the telescopic end of the arc-shaped telescopic plate (2). A sensor is embedded on one side of the lower surface of the fixing plate (201). A sensor head (103) is embedded on one side of the upper surface of the placement plate (1). The sensor head (103) cooperates with the sensor. A controller is installed on one side of the inner top wall of the protective shell (302).

9. A culture system for a closed mixotrophic culture method of microalgae according to any one of claims 1 to 8, characterized in that: The controller is provided with a main control module, a detection module and a wireless connection module. The detection module is used to detect various data and send the detection data to the main control module to facilitate the control of the culture device. The main control module is used to receive signals sent by the sensor and the detection module, and control the first electric telescopic rod (202), the vacuum pump (401), the drive motor (507) and the second electric telescopic rod (602) according to the signal content. The wireless connection module is used to connect to the user's terminal through a wireless network to facilitate the implementation of understanding the culture situation.

10. A closed mixotrophic culture method and culture system of microalgae according to claim 9, characterized in that: The detection module comprises an environment detection unit, a carbon dioxide detection unit and a light detection unit. The environment detection unit is used to sense sunlight and judge that it is daytime when a set interval value is reached. The carbon dioxide detection unit is used to detect the carbon dioxide content in the culture tank (3). The light detection unit is used to detect the light intensity in the culture tank (3).

Images