A device for separating fluid from frozen animal lymphoid tissue and its working method

Abstract

This invention discloses a liquid separation device for frozen animal lymphoid tissue and its operating method. The device includes a separation chamber. A mounting shaft is located at the top of the separation chamber via a first motor, which drives the mounting shaft to rotate. The middle of the mounting shaft is rotatably connected to a liquid-filling tube via a fixing plate. The liquid-filling tube has a through hole and a liquid outlet at its bottom. A push rod is installed in the through hole, with one end extending into the liquid-filling tube and connecting to the bottom of a separating filter plate. A sealing rod is installed in the liquid outlet, with one end extending into the liquid-filling tube and connecting to the bottom of the separating filter plate. Inside the separation chamber, along the rotation direction of the fixing plate, are sequentially arranged a decomposition component, a centrifugation component, a liquid separation component, and a cleaning component. The decomposition component is used to thaw the frozen lymphoid tissue. The centrifugation component drives the liquid-filling tube to rotate. The liquid separation component separates the liquid within the liquid-filling tube. The cleaning component cleans the liquid-filling tube and the separating filter plate. This invention provides convenient operation and improves the integrity of lymphoid tissue cells.

Description

Technical Field

[0001] This invention belongs to the field of cell separation technology, specifically relating to a device for separating fluid from frozen animal lymphoid tissue and its working method. Background Technology

[0002] Animal lymphoid tissue plays a crucial role in experimental medicine, primarily used to study the function of the immune system and develop disease models. Experiments with animal lymphoid tissue also include lymphocyte transformation assays, a method for assessing the state of cellular immune function. This involves adding appropriate stimuli during in vitro T cell culture and observing the transformation of lymphocytes to evaluate the body's cellular immune function. The application of these experimental techniques provides effective means to study the response and regulatory mechanisms of the immune system. However, current techniques for separating lymphocytes require mechanical decomposition of the lymphoid tissue. After decomposition, the lymphoid tissue needs to be placed in a reaction tube, and after the reaction, the solid lymphoid tissue needs to be separated. Most existing separation methods simply separate the lymphoid tissue from the liquid using filters or other components, leaving a significant amount of liquid residue within the lymphoid tissue. Directly discarding this residue results in liquid waste, making the current separation techniques cumbersome and inefficient.

[0003] Furthermore, animal lymphoid tissue contains fluids such as lymph. To separate the cells from the internal fluids of lymphoid tissue, the animal lymphoid tissue needs to be frozen when it is obtained. However, the current method mostly involves cutting the frozen animal lymphoid tissue after it has softened slightly, and then placing the tissue in a container until it is completely thawed before the experiment can be conducted. This method wastes a lot of time when separating the fluids from frozen animal lymphoid tissue, and the constant transfer of the tissue between various containers makes the frozen lymphoid tissue susceptible to contamination, thus causing the failure of the lymphoid tissue cell-fluid separation.

[0004] Based on this, the present invention provides a frozen animal lymphatic tissue liquid separation device and its working method, which can eliminate the drawbacks of existing devices. Summary of the Invention

[0005] Technical problem to be solved: In view of the above-mentioned technical problems, the present invention provides a frozen animal lymphatic tissue liquid separation device and its working method, which can effectively solve the shortcomings of the prior art in terms of cumbersome separation operation and low separation efficiency.

[0006] Technical Solution: In a first aspect, the present invention provides a liquid separation device for frozen animal lymphoid tissue, comprising a separation chamber. A mounting shaft is mounted on the top of the separation chamber via a first motor, which drives the mounting shaft to rotate. The middle of the mounting shaft is rotatably connected to a liquid-filling tube via a fixing plate. A through hole and a liquid outlet are provided at the bottom of the liquid-filling tube. A push rod is provided within the through hole, one end of which extends into the interior of the liquid-filling tube and connects to the bottom of a separating filter plate. A sealing rod is provided within the liquid outlet, one end of which extends into the interior of the liquid-filling tube and connects to the bottom of the separating filter plate. Inside the separation chamber, along the rotation direction of the fixing plate, a decomposition component, a centrifugation component, a liquid separation component, and a cleaning component are sequentially arranged. The decomposition component is used to thaw the frozen lymphoid tissue. The centrifugation component is used to drive the liquid-filling tube to rotate. The liquid separation component is used to separate the liquid within the liquid-filling tube. The cleaning component is used to clean the liquid-filling tube and the separating filter plate.

[0007] Preferably, the decomposition component includes a decomposition tube, the bottom of which is a decomposition mesh. The bottom of the decomposition mesh is connected to the output end of an electric push rod via a support tube passing through the decomposition tube. A heating component is provided inside the support tube and is electrically connected to an external control component. The electric push rod is located at the top inside the separation chamber. An installation tube is provided at the output end of the electric push rod. A fixed decomposition plate is provided at the bottom end of the installation tube. The outer edge of the fixed decomposition plate is slidably connected to the inner wall of the decomposition tube. The upper end of the fixed decomposition plate is connected to the top inside the separation chamber via several support rods. A first chamber door is provided on the side wall of the separation chamber located at the decomposition tube. A rotating assembly for driving the liquid-filling tube to rotate is provided inside the separation chamber.

[0008] Furthermore, the rotating assembly includes a friction ring sleeved on the outer wall of the liquid-filling tube. A first friction wheel is provided inside the separation chamber, and the first friction wheel is in close friction with the friction ring. The first friction wheel is located at one end of a first mounting shaft, on which a plurality of first rotating plates are rotatably mounted. The first rotating plates are fixedly mounted inside the separation chamber. A first driven wheel is provided at the other end of the first mounting shaft, and the first driven wheel is connected to a first driving wheel via a first belt. The first driving wheel is fixedly mounted on the output end of a second motor, and the second motor is fixedly mounted at the bottom end inside the separation chamber.

[0009] Preferably, the centrifugal component includes a second friction wheel, which is fixedly mounted on one end of a second mounting shaft. A plurality of second rotating plates are rotatably mounted on the second mounting shaft. The second rotating plates are fixedly mounted inside the separation chamber. A second driven wheel is provided at the other end of the second mounting shaft. The second driven wheel is connected to a second driving wheel via a second belt. The second driving wheel is fixedly mounted on the output end of a second motor. A first sealing plate is provided above the second friction wheel at a position corresponding to the liquid filling pipe. The first sealing plate is fixedly mounted on the inner side wall of the separation chamber. The lower surface of the first sealing plate is in close contact with the upper end of the liquid filling pipe.

[0010] Preferably, the liquid separation component includes a first guide plate, which is disposed at the bottom of the separation chamber and corresponds to the other end of the push rod. A top column is provided between the push rod and the first guide plate, and the top column is connected to the push rod by a return spring. A liquid collection pipe is provided on one side of the first guide plate at the position corresponding to the liquid outlet. The liquid collection pipe is disposed on the side wall of the separation chamber, and a drain pipe is connected to the bottom end of the liquid collection pipe. One end of the drain pipe extends to the outside of the separation chamber. A correction mechanism is provided inside the separation chamber at the position corresponding to the first guide plate. The correction mechanism is used to correct the position of the liquid filling pipe.

[0011] Furthermore, the calibration mechanism includes a calibration friction ring and a calibration plate. The calibration plate is positioned above one end of the first guide plate and connected to the inner wall of the separation chamber via several spring telescopic rods. A calibration friction ring is fixedly provided on the outer wall of the liquid filling tube at a position corresponding to the calibration plate. The calibration friction ring has a planar notch, and rollers are provided at both ends of the planar notch. The arc-shaped surface of the calibration friction ring is rubbed tightly against the calibration plate. A second sealing plate is provided above the first guide plate at a position corresponding to the liquid filling tube. The bottom end of the second sealing plate is at the same height as the top end of the liquid filling tube. The second sealing plate is fixedly provided on the inner wall of the separation chamber.

[0012] Furthermore, the cleaning component includes a cleaning nozzle, which is fixedly installed inside the separation box. The bottom of the cleaning nozzle has several spray holes. The water inlet of the cleaning nozzle is connected to the water outlet of an external water supply device through a water inlet pipe. A control component is provided at the connection between the water outlet of the water inlet pipe and the water inlet of the cleaning nozzle. The control component is used to control the opening and closing of the water inlet of the cleaning nozzle. A second guide plate is provided below the cleaning nozzle. The second guide plate is located at the bottom of the separation box and directly below the top column. A collection box is provided between the second guide plate and the bottom of the separation box. A filter screen is provided inside the collection box.

[0013] Furthermore, the control component includes a ball valve, the ball valve inlet being connected to the water outlet of the inlet pipe, the ball valve outlet being connected to the water inlet of the cleaning nozzle, the ball valve being fixedly mounted inside the top of the separation box by a support frame, a third friction wheel being provided at one end of the valve stem of the ball valve, a friction plate being provided in close friction on the third friction wheel, and the friction plate being fixedly mounted on the upper surface of the fixed plate.

[0014] Preferably, a scraper is provided below the cleaning nozzle, one end of which is fixedly mounted on the inner wall of the separation tank, and the lower surface of the scraper is in close contact with the upper surface of the liquid filling pipe.

[0015] In a second aspect, the present invention provides a method for operating the frozen animal lymphatic tissue fluid separation device described in the first aspect, comprising the following steps:

[0016] S1. Place small-volume coarse material in the decomposition tube, add decomposition liquid to the liquid filling tube, start the electric push rod to immerse the decomposition tube in the decomposition liquid in the liquid filling tube, turn on the heating component in the support tube to allow the decomposition liquid to complete the initial decomposition of lymph tissue, start the second motor to drive the liquid filling tube to rotate at low speed, after the initial decomposition is completed, the electric push rod drives the decomposition tube to rise, and the electric push rod and the decomposition net crush the small-volume coarse material into fine lymph tissue that falls onto the isolation filter plate.

[0017] S2. The first motor drives the liquid filling tube to rotate 90 degrees for the first time. The liquid filling tube rotates to the bottom of the first sealing plate and is in close contact with the bottom of the first sealing plate. The second friction wheel drives the liquid filling tube to rotate at high speed through the friction ring.

[0018] S3. The first motor drives the liquid-filling tube to rotate 90 degrees for the second time. The two rollers at the plane notch are in close contact with the correction plate, the liquid outlet is aligned with the liquid collection tube, the isolation filter plate pushes the refined lymph tissue to move, and under the action of the isolation filter plate and the second sealing plate, the refined lymph tissue is squeezed. The decomposition liquid inside the liquid-filling tube is discharged into the liquid collection tube through the liquid outlet, and the liquid collection tube discharges the decomposition liquid through the liquid discharge pipe.

[0019] S4. The first motor drives the liquid filling tube to rotate 90 degrees for the third time, and the bottom end of the top column is in close contact with the upper end of the second guide plate. The scraper scrapes off the lymph tissue on the upper end of the isolation filter plate. The friction plate is in close contact with the third friction wheel, so that the third friction wheel drives the ball to rotate. The external water supply device supplies water to the inside of the water inlet pipe. The cleaning nozzle sprays water out through the spray hole to clean the isolation filter plate and the inside of the liquid filling tube.

[0020] Beneficial effects: This invention, by sequentially arranging a decomposition component, a centrifugation component, a liquid separation component, and a cleaning component inside the separation chamber along the rotation direction of the fixed plate, facilitates the completion of the corresponding process after the first motor drives the liquid-filling tube to rotate 90 degrees each time, simplifying operation. The decomposition liquid first decomposes small volumes of coarse material, and then the decomposition tube, in conjunction with the fixed decomposition plate, compresses the small volumes of coarse material into fine lymphoid tissue. Centrifugation then separates the decomposition liquid from the fine lymphoid tissue, effectively avoiding the direct destruction of lymphoid tissue cells through fine cutting, thus improving the integrity of lymphoid tissue cells. The liquid-filling tube, isolation filter plate, push rod, and top column, used in conjunction with the decomposition component, centrifugation component, and liquid separation component respectively, improve separation efficiency. After separation, the cleaning component cleans the liquid-filling tube and isolation filter plate for subsequent use, thereby increasing the practicality of the frozen animal lymphoid tissue liquid separation device. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of a frozen animal lymphatic tissue fluid separation device according to the present invention;

[0022] Figure 2 This is a schematic diagram of the electric push rod structure in a frozen animal lymphatic tissue fluid separation device of the present invention;

[0023] Figure 3 This is a schematic diagram of the support tube structure in a frozen animal lymphatic tissue fluid separation device of the present invention;

[0024] Figure 4 This is a schematic diagram of the structure of the second friction wheel and the first sealing plate in a frozen animal lymphatic tissue liquid separation device of the present invention;

[0025] Figure 5 This is a schematic diagram of the calibration plate and collection tube structure in a frozen animal lymphatic tissue fluid separation device of the present invention;

[0026] Figure 6 This is a schematic diagram of the liquid discharge state of the liquid loading tube in a frozen animal lymphatic tissue liquid separation device of the present invention;

[0027] Figure 7 This is a schematic diagram showing the position of the ball valve in a frozen animal lymphatic tissue fluid separation device of the present invention;

[0028] Figure 8 This is a schematic diagram of the scraper structure in a frozen animal lymphatic tissue liquid separation device of the present invention;

[0029] Figure 9 This is a schematic diagram of the liquid loading tube structure in a frozen animal lymphatic tissue liquid separation device of the present invention;

[0030] The following are the serial numbers in the diagram: 11. Separation box, 12. Mounting shaft, 13. First motor, 14. Fixing plate, 15. Liquid filling pipe, 16. Isolation filter plate, 17. Push rod, 18. Top column, 19. Return spring, 20. Sealing rod, 21. Friction ring, 22. Correcting friction ring, 23. Decomposition pipe, 24. Support pipe, 25. Electric push rod, 26. Fixed decomposition plate, 27. First friction wheel, 28. Second motor, 29. Second friction wheel, 30. First sealing plate, 31. Correcting plate, 32. Spring telescopic rod, 33. First guide plate, 34. Liquid collecting pipe, 35. Second sealing plate, 36. Scraper frame, 37. Second guide plate, 38. Cleaning nozzle, 39. Water inlet pipe, 40. Ball valve, 41. Third friction wheel, 42. Friction plate, 43. Collection box. Detailed Implementation

[0031] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments: Example 1

[0032] like Figure 1-9 As shown, a frozen animal lymphatic tissue liquid separation device includes a separation chamber 11. A mounting shaft 12 is located at the top of the separation chamber 11 via a first motor 13, which drives the mounting shaft 12 to rotate. The middle of the mounting shaft 12 is rotatably connected to a liquid-filling tube 15 via a fixing plate 14. The bottom of the liquid-filling tube 15 has a through hole and a liquid outlet hole. A push rod 17 is located in the through hole, with one end extending into the liquid-filling tube 15 and connecting to the bottom of an isolation filter plate 16. A sealing rod 20 is located in the liquid outlet hole, with one end extending into the liquid-filling tube 15 and connecting to the bottom of the isolation filter plate 16. Inside the separation chamber 11, along the rotation direction of the fixing plate 14, are sequentially arranged a decomposition component, a centrifugation component, a liquid separation component, and a cleaning component. The decomposition component is used to thaw the frozen lymphatic tissue. The centrifugation component is used to drive the liquid-filling tube 15 to rotate. The liquid separation component is used to separate the liquid within the liquid-filling tube 15. The cleaning component is used to clean the liquid-filling tube 15 and the isolation filter plate 16.

[0033] The aforementioned decomposition components include a decomposition tube 23, the bottom end of which is a decomposition mesh. Both the decomposition tube 23 and the decomposition mesh are made of thermally conductive material. The bottom of the decomposition mesh is connected to the output end of an electric push rod 25 via a support tube 24 that passes through the decomposition tube 23. A heating component is provided inside the support tube 24 to heat the decomposition mesh and the decomposition tube 23 without damaging the lymphatic tissue cells. The heating component is electrically connected to an external control component. The electric push rod 25 is located at the top inside the separation box 11. An installation tube is provided at the output end of the electric push rod 25. A fixed decomposition plate 26 is provided at the bottom end of the installation tube. The outer edge of the fixed decomposition plate 26 is slidably connected to the inner wall of the decomposition tube 23. The upper end of the fixed decomposition plate 26 is connected to the top inside the separation box 11 via several support rods. A first door is provided on the side wall of the separation box 11 located at the decomposition tube 23. A rotating assembly for driving the liquid-filling tube 15 to rotate is provided inside the separation box 11. In use, softened lymphoid tissue is cut into small coarse pieces using an external cutting component. The first chamber door is then opened, and the small coarse pieces are placed inside the decomposition tube 23. An external control component activates the heating element inside the support tube 24. Both the decomposition mesh and the decomposition tube 23 are made of heat-conducting material, allowing the heating element to heat them without damaging the cells inside the lymphoid tissue. Simultaneously, decomposition fluid is added to the liquid-filling tube 15. After the small coarse pieces inside the decomposition tube 23 have thawed, the electric push rod 25 is activated. The output of the electric push rod 25 drives the decomposition tube 23... 3. The movement allows the decomposition tube 23 to be immersed in the decomposition liquid inside the liquid-filling tube 15, so that the decomposition liquid can initially decompose the lymphoid tissue. At the same time, the rotating component drives the liquid-filling tube 15 to rotate at a low speed, thereby accelerating the decomposition of the lymphoid tissue by the decomposition liquid. After the initial decomposition is completed, the output end of the electric push rod 25 drives the decomposition tube 23 to rise, so that the small volume of coarse material inside the decomposition tube 23 is in close contact with the bottom end of the fixed decomposition plate 26. Under the action of the electric push rod 25 and the decomposition net, the small volume of coarse material is crushed into fine lymphoid tissue. The fine lymphoid tissue falls onto the upper end of the isolation filter plate 16 for separation experiments.

[0034] The aforementioned rotating assembly includes a friction ring 21, which is sleeved on the outer wall of the liquid-filling tube 15. A first friction wheel 27 is provided inside the separation chamber 11, and the first friction wheel 27 is in close friction with the friction ring 21. The first friction wheel 27 is located at one end of a first mounting shaft, on which a plurality of first rotating plates are rotatably mounted. The first rotating plates are fixedly mounted inside the separation chamber 11. A first driven wheel is located at the other end of the first mounting shaft, and the first driven wheel is connected to a first driving wheel via a first belt. The first driving wheel is fixedly mounted on the output end of a second motor 28, which is fixedly mounted at the bottom end inside the separation chamber 11. In use, when preliminary decomposition of small-volume coarse materials is required, the second motor 28 is started. The second motor 28 drives the first friction wheel 27 to rotate via the first belt. Because the first friction wheel 27 is in close friction with the friction ring 21, the first friction wheel 27 drives the liquid-filling tube 15 to rotate at a low speed via the friction ring 21, thereby accelerating the decomposition of lymphatic tissue by the decomposition liquid.

[0035] The centrifugal component includes a second friction wheel 29, which is fixedly mounted on one end of a second mounting shaft. Several second rotating plates are rotatably mounted on the second mounting shaft. The second rotating plates are fixedly mounted inside the separation chamber 11. A second driven wheel is provided at the other end of the second mounting shaft. The second driven wheel is connected to a second driving wheel via a second belt. The second driving wheel is fixedly mounted on the output end of a second motor 28. A first sealing plate 30 is provided above the second friction wheel 29 at a position corresponding to the liquid filling pipe 15. The first sealing plate 30 is fixedly mounted on the inner side wall of the separation chamber 11. The lower surface of the first sealing plate 30 is in close contact with the upper end of the liquid filling pipe 15.

[0036] The aforementioned liquid separation component includes a first guide plate 33, which is disposed at the bottom of the separation chamber 11 and corresponds to the other end of the push rod 17. A top post 18 is provided between the push rod 17 and the first guide plate, and the top post 18 is connected to the push rod 17 through a return spring 19. A liquid collection pipe 34 is provided on one side of the first guide plate 33 at the position corresponding to the liquid outlet hole. The liquid collection pipe 34 is disposed on the side wall of the separation chamber 11, and a drain pipe is connected to the bottom end of the liquid collection pipe 34. One end of the drain pipe extends to the outside of the separation chamber 11. A correction mechanism is provided inside the separation chamber 11 at the position corresponding to the first guide plate 33. The correction mechanism is used to correct the position of the liquid filling pipe 15.

[0037] The aforementioned calibration mechanism includes a calibration friction ring 22 and a calibration plate 31. The calibration plate 31 is positioned above one end of the first guide plate 33 and is connected to the inner wall of the separation box 11 via several spring telescopic rods 32. The calibration friction ring 22 is fixedly provided on the outer wall of the liquid filling tube 15 at a position corresponding to the calibration plate 31. The calibration friction ring 22 has a planar notch, and rollers are provided at both ends of the planar notch. The arc-shaped surface of the calibration friction ring 22 is rubbed tightly against the calibration plate 31. A second sealing plate 35 is provided above the first guide plate 33 at a position corresponding to the liquid filling tube 15. The bottom end of the second sealing plate 35 is at the same height as the top end of the liquid filling tube 15. The second sealing plate 35 is fixedly provided on the inner wall of the separation box 11. In use, after the decomposing liquid inside the liquid-filling tube 15 has finished centrifuging and decomposing the fine lymphoid tissue, the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees again, so that the liquid-filling tube 15 rotates to the bottom end of the second sealing plate 35. During this process, the correction friction ring 22 first contacts the correction plate 31. When the arc-shaped surface of the correction friction ring 22 is released from the correction plate 31, the correction friction ring 22 rotates due to the rotation driven by the liquid-filling tube 15, and the correction friction ring 22 rubs tightly against the correction plate 31, causing the correction friction ring 22 to rotate. When one end of the notch on the plane of the correction friction ring 22 is tightly pressed against one side of the correction plate 31, under the action of several spring telescopic rods 32, the two rollers at the notch are pressed tightly against the correction plate 31, so that the correction friction ring 22 stops rotating. At the same time, the bottom end of the liquid-filling tube 15 exits... The liquid hole is aligned with the liquid collection tube 34, and the top column 18 is in close contact with the inclined surface of the first guide plate 33. When the mounting shaft 12 drives the liquid filling tube 15 to rotate, one end of the top column 18 moves on the inclined surface of the first guide plate 33, so that the top column 18 pushes the isolation filter plate 16 to move through the push rod 17. The isolation filter plate 16 pushes the refined lymph tissue to move. When one end of the top column 18 is in close contact with the uppermost end of the first guide plate 33, the upper end of the isolation filter plate 16 and the bottom end of the second sealing plate 35 act to squeeze the refined lymph tissue, thereby separating the liquid generated inside the refined lymph tissue. At the same time, the sealing rod 20 disengages from the liquid outlet hole, and the decomposed liquid inside the liquid filling tube 15 is discharged into the liquid collection tube 34 through the liquid outlet hole. The liquid collection tube 34 discharges the decomposed liquid through the liquid discharge pipe, which facilitates subsequent experimental research.

[0038] The cleaning components include a cleaning nozzle 38, which is fixedly installed inside the separation box 11. The bottom of the cleaning nozzle 38 has several spray holes. The water inlet of the cleaning nozzle 38 is connected to the water outlet of an external water supply device through a water inlet pipe 39. A control component is provided at the connection between the water outlet of the water inlet pipe 39 and the water inlet of the cleaning nozzle 38. The control component is used to control the opening and closing of the water inlet of the cleaning nozzle 38. A second guide plate 37 is provided below the cleaning nozzle 38. The second guide plate 37 is located at the bottom of the separation box 11 and directly below the top column 18. A collection box 43 is provided between the second guide plate 37 and the bottom of the separation box 11. A filter screen is provided inside the collection box 43. In use, after the decomposition liquid inside the liquid-filling tube 15 has been drained, the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees again, so that the liquid-filling tube 15 rotates to below the cleaning nozzle 38. During this process, the bottom end of the top column 18 first comes into close contact with the inclined surface of the second guide plate 37. Under the guidance of the second guide plate 37, the top column 18 pushes the isolation filter plate 16 to slide inside the liquid-filling tube 15 through the push rod 17. When one end of the top column 18 comes into close contact with the upper end of the second guide plate 37, the isolation filter plate 16 and one end of the liquid-filling tube 15 are on the same plane. Then, water is supplied to the inside of the water inlet pipe 39 through the external water supply component. The water inlet pipe 39 delivers the water source to the inside of the cleaning nozzle 38. The cleaning nozzle 38 sprays water through several spray holes to clean the isolation filter plate 16 and the inside of the liquid-filling tube 15. The connection between the output end of the water inlet pipe 39 and the input end of the cleaning nozzle 38 is provided with a control component for controlling the switch of the input end of the cleaning nozzle 38.

[0039] The aforementioned control components include a ball valve 40, the inlet of which is connected to the outlet of the inlet pipe 39, and the outlet of which is connected to the inlet of the cleaning nozzle 38. The ball valve 40 is fixedly mounted inside the top of the separation box 11 by a support frame. One end of the valve stem of the ball valve 40 is provided with a third friction wheel 41, and a friction plate 42 is provided in close friction on the third friction wheel 41. The friction plate 42 is fixedly mounted on the upper surface of the fixing plate 14. In use, when the fixed plate 14 drives the liquid-filling pipe 15 to rotate 90 degrees to below the cleaning nozzle 38, the friction plate 42 and the third friction wheel 41 rub against each other, causing the third friction wheel 41 to drive the ball to rotate 90 degrees, so that the ball valve 40 is in the conducting state. After cleaning is completed, the first motor 13 drives the liquid-filling pipe 15 to rotate 90 degrees again to below the decomposition pipe 23. During this process, the friction plate 42 continues to rub against the third friction wheel 41, causing the ball to rotate 90 degrees again, so that the ball valve 40 is in the closed state. At the same time, the bottom of the separation box 11 and the bottom of the decomposition pipe 23 are also equipped with a wastewater tank for collecting wastewater, which is not shown in the figure.

[0040] Below the cleaning nozzle 38 is a scraper 36. One end of the scraper 36 is fixedly mounted on the inner wall of the separation box 11, and the lower surface of the scraper 36 is in close contact with the upper end of the liquid filling pipe 15. In use, when the liquid filling pipe 15 rotates to the position below the scraper 36, one end of the top column 18 is in close contact with the upper end of the second guide plate 37, causing the push rod 17 to push the upper end of the isolation filter plate 16 to be in close contact with the bottom end of the scraper 36. As the liquid filling pipe 15 continues to rotate, the scraper 36 scrapes the lymph tissue off the upper end of the isolation filter plate 16, causing the lymph tissue to fall into the collection box 43. Example 2

[0041] The working method of the frozen animal lymphatic tissue fluid separation device described in Example 1 includes the following steps:

[0042] S1. The softened lymphoid tissue is cut into small, coarse pieces. The first chamber door is then opened, and the small pieces are placed inside the decomposition tube 23. An external control unit activates the heating element inside the support tube 24. Both the decomposition mesh and the decomposition tube 23 are made of heat-conducting materials, allowing the heating element to heat them without damaging the cells inside the lymphoid tissue. Simultaneously, decomposition fluid is added to the liquid-filling tube 15. After the small pieces inside the decomposition tube 23 have thawed, the electric push rod 25 is activated. The output of the electric push rod 25 moves the decomposition tube 23, immersing it in the decomposition fluid in the liquid-filling tube 15. Inside the liquid, the decomposition liquid initially decomposes the lymphoid tissue. At the same time, the second motor 28 is started. The second motor 28 drives the first friction wheel 27 to rotate through the first belt. Since the first friction wheel 27 is in close contact with the friction ring 21, the first friction wheel 27 drives the liquid-filling tube 15 to rotate at a low speed through the friction ring 21. After the initial decomposition is completed, the output end of the electric push rod 25 drives the decomposition tube 23 to rise, so that the small volume of coarse material inside the decomposition tube 23 is in close contact with the bottom end of the fixed decomposition plate 26. Under the action of the electric push rod 25 and the decomposition net, the small volume of coarse material is crushed into fine lymphoid tissue, and the fine lymphoid tissue falls onto the upper end of the isolation filter plate 16.

[0043] S2. The output end of the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees for the first time, so that the liquid-filling tube 15 rotates to the bottom of the first sealing plate 30, and the upper end of the liquid-filling tube 15 is in close contact with the bottom end of the first sealing plate 30. At the same time, the friction ring 21 and the second friction wheel 29 are in close contact. Then, when the second friction wheel 29 rotates, it drives the liquid-filling tube 15 to rotate at high speed through the friction ring 21, which accelerates the separation of the decomposition liquid from the fine lymph tissue.

[0044] S3. The first motor 13 drives the liquid-filling pipe 15 to rotate 90 degrees for the second time, causing the liquid-filling pipe 15 to rotate to the bottom end of the second sealing plate 35. During this process, the correction friction ring 22 first contacts the correction plate 31. When the arc-shaped surface of the correction friction ring 22 contacts the correction plate 31, the correction friction ring 22 rotates due to the rotation driven by the liquid-filling pipe 15, and the correction friction ring 22 rubs tightly against the correction plate 31, causing the correction friction ring 22 to rotate. When one end of the notch on the plane of the correction friction ring 22 is tightly against one side of the correction plate 31, under the action of several spring telescopic rods 32, the two rollers at the notch are tightly against the correction plate 31, so that the correction friction ring 22 stops rotating. At the same time, the liquid outlet at the bottom end of the liquid-filling pipe 15 aligns with the liquid collection pipe 34. When the top column 18 is in close contact with the inclined surface of the first guide plate 33, and the mounting shaft 12 drives the liquid-filling tube 15 to rotate, one end of the top column 18 moves on the inclined surface of the first guide plate 33, so that the top column 18 pushes the isolation filter plate 16 to move through the push rod 17. The isolation filter plate 16 pushes the refined lymph tissue to move. When one end of the top column 18 is in close contact with the uppermost end of the first guide plate 33, under the action of the upper end of the isolation filter plate 16 and the bottom end of the second sealing plate 35, the refined lymph tissue is squeezed, thereby separating the residual liquid inside the refined lymph tissue. At the same time, the sealing rod 20 disengages from the liquid outlet hole, and the decomposed liquid inside the liquid-filling tube 15 is discharged into the liquid collection tube 34 through the liquid outlet hole. The liquid collection tube 34 discharges the decomposed liquid through the liquid discharge tube, which is convenient for subsequent experimental research.

[0045] S4. The first motor 13 drives the liquid-filling pipe 15 to rotate 90 degrees for the third time, so that the liquid-filling pipe 15 rotates to below the cleaning nozzle 38. During this process, the bottom end of the top column 18 first comes into close contact with the inclined surface of the second guide plate 37. Under the guidance of the second guide plate 37, the top column 18 pushes the isolation filter plate 16 to slide inside the liquid-filling pipe 15 through the push rod 17. When one end of the top column 18 comes into close contact with the upper end of the second guide plate 37, the isolation filter plate 16 and one end of the liquid-filling pipe 15 are on the same plane. The scraper 36 scrapes the lymph tissue off the upper end of the isolation filter plate 16, so that the lymph tissue falls into the collection box 43. Inside, the friction plate 42 and the third friction wheel 41 rub against each other, causing the third friction wheel 41 to rotate the ball by 90 degrees, so that the ball valve 40 is in the conducting state. Then, water is supplied to the inside of the water inlet pipe 39 through the external water supply component. The water inlet pipe 39 delivers the water source to the inside of the cleaning nozzle 38. The cleaning nozzle 38 sprays water out through several spray holes, so as to clean the inside of the isolation filter plate 16 and the liquid filling pipe 15. The first motor 13 drives the liquid filling pipe 15 to rotate 90 degrees for the fourth time, so that the cleaned liquid filling pipe 15 rotates to the position below the decomposition pipe 23 for the next separation experiment.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A device for separating fluid from frozen animal lymphatic tissue, characterized in that: The system includes a separation box (11). The top of the separation box (11) is equipped with an installation shaft (12) via a first motor (13). The first motor (13) drives the installation shaft (12) to rotate. The middle of the installation shaft (12) is rotatably connected to a liquid-filling pipe (15) via a fixing plate (14). The bottom of the liquid-filling pipe (15) has a through hole and a liquid outlet hole. A push rod (17) is installed inside the through hole. One end of the push rod (17) extends into the interior of the liquid-filling pipe (15) and connects to the bottom of a separating filter plate (16). The liquid outlet hole has a dense... A sealing rod (20) is provided, one end of which extends into the interior of the liquid-filling tube (15) and connects to the bottom of the isolation filter plate (16). The separation box (11) is provided with a decomposition component, a centrifugation component, a liquid separation component and a cleaning component in sequence along the rotation direction of the fixed plate (14). The decomposition component is used to thaw frozen lymph tissue. The centrifugation component is used to drive the liquid-filling tube (15) to rotate. The liquid separation component is used to separate the liquid in the liquid-filling tube (15). The cleaning component is used to clean the liquid-filling tube (15) and the isolation filter plate (16). The decomposition component includes a decomposition tube (23), the bottom of which is a decomposition mesh. The bottom of the decomposition mesh is connected to the output end of an electric push rod (25) through a support tube (24) that passes through the decomposition tube (23). The support tube (24) is equipped with a heating component, which is electrically connected to an external control component. The electric push rod (25) is located at the top inside the separation box (11). The output end of the electric push rod (25) is equipped with an installation tube. The bottom end of the installation tube is equipped with a fixed decomposition plate (26). The outer edge of the fixed decomposition plate (26) is slidably connected to the inner wall of the decomposition tube (23). The upper end of the fixed decomposition plate (26) is connected to the top inside the separation box (11) through several support rods. The separation box (11) has a first door on the side wall located at the decomposition tube (23). The separation box (11) is equipped with a rotating component for driving the liquid-filling tube (15) to rotate.

2. The frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The rotating assembly includes a friction ring (21) which is sleeved on the outer wall of the liquid-filling tube (15). The separation box (11) is provided with a first friction wheel (27) which is frictionally attached to the friction ring (21). The first friction wheel (27) is located at one end of a first mounting shaft. A plurality of first rotating plates are rotatably mounted on the first mounting shaft. The first rotating plates are fixedly mounted inside the separation box (11). The other end of the first mounting shaft is provided with a first driven wheel. The first driven wheel is connected to a first driving wheel through a first belt. The first driving wheel is fixedly mounted on the output end of a second motor (28). The second motor (28) is fixedly mounted at the bottom end inside the separation box (11).

3. The frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The centrifugal component includes a second friction wheel (29), which is fixedly mounted on one end of a second mounting shaft. Several second rotating plates are rotatably mounted on the second mounting shaft. The second rotating plates are fixedly mounted inside the separation box (11). A second driven wheel is provided at the other end of the second mounting shaft. The second driven wheel is connected to the second driving wheel via a second belt. The second driving wheel is fixedly mounted on the output end of the second motor (28). A first sealing plate (30) is provided above the second friction wheel (29) at a position corresponding to the liquid filling pipe (15). The first sealing plate (30) is fixedly mounted on the inner side wall of the separation box (11). The lower surface of the first sealing plate (30) is in close contact with the upper end of the liquid filling pipe (15).

4. The frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The liquid separation component includes a first guide plate (33), which is located at the bottom of the separation tank (11) and corresponds to the other end of the push rod (17). A top post (18) is provided between the push rod (17) and the first guide plate. The top post (18) is connected to the push rod (17) via a return spring (19). A liquid collection pipe (34) is provided on one side of the first guide plate (33) at the position corresponding to the liquid outlet. The liquid collection pipe (34) is located on the side wall of the separation tank (11). A drain pipe is connected to the bottom end of the liquid collection pipe (34). One end of the drain pipe extends to the outside of the separation tank (11). A correction mechanism is provided inside the separation tank (11) at the position corresponding to the first guide plate (33). The correction mechanism is used to correct the position of the liquid filling pipe (15). The first guide plate (33) is located above the liquid filling pipe. (15) A second sealing plate (35) is provided at the corresponding position. The bottom end of the second sealing plate (35) is at the same height as the top end of the liquid filling pipe (15). The second sealing plate (35) is fixedly installed on the inner side wall of the separation box (11). The top column (18) is in close contact with the inclined surface of the first guide plate (33). When the mounting shaft (12) drives the liquid filling pipe (15) to rotate, one end of the top column (18) moves on the inclined surface of the first guide plate (33), so that the top column (18) pushes the isolation filter plate (16) to move through the push rod (17). The isolation filter plate (16) pushes the refined lymph tissue to move. When one end of the top column (18) is in close contact with the uppermost end of the first guide plate (33), under the action of the upper end of the isolation filter plate (16) and the bottom end of the second sealing plate (35), the refined lymph tissue is squeezed, thereby separating the liquid generated inside the refined lymph tissue.

5. The frozen animal lymphatic tissue liquid separation device according to claim 4, characterized in that: The calibration mechanism includes a calibration friction ring (22) and a calibration plate (31). The calibration plate (31) is located above one end of the first guide plate (33) and is connected to the inner wall of the separation box (11) through several spring telescopic rods (32). The calibration friction ring (22) is fixedly provided on the outer wall of the liquid filling tube (15) at the corresponding position of the calibration plate (31). The calibration friction ring (22) has a planar notch, and rollers are provided at both ends of the planar notch. The arc-shaped surface of the calibration friction ring (22) is rubbed tightly against the calibration plate (31).

6. The frozen animal lymphatic tissue liquid separation device according to claim 4, characterized in that: The cleaning component includes a cleaning nozzle (38), which is fixedly installed inside the separation box (11). The bottom of the cleaning nozzle (38) has several spray holes. The water inlet of the cleaning nozzle (38) is connected to the outlet of an external water supply device via a water inlet pipe (39). A control component is provided at the connection between the outlet of the water inlet pipe (39) and the water inlet of the cleaning nozzle (38). The control component is used to control the opening and closing of the water inlet of the cleaning nozzle (38). A second guide plate (37) is provided below the cleaning nozzle (38). The second guide plate (37) is located at the bottom of the separation box (11) and directly below the top column (18). The second guide plate (37) is connected to the separation box (11)... A collection box (43) is provided between the bottom of the box (11), and a filter screen is provided inside the collection box (43). After the decomposed liquid in the liquid filling tube (15) is drained, the first motor (13) drives the liquid filling tube (15) to rotate again, so that the liquid filling tube (15) rotates to the bottom of the cleaning nozzle (38). During this process, the bottom end of the top column (18) first comes into close contact with the inclined surface of the second guide plate (37). Under the guidance of the second guide plate (37), the top column (18) pushes the isolation filter plate (16) to slide inside the liquid filling tube (15) through the push rod (17). When one end of the top column (18) comes into close contact with the upper end of the second guide plate (37), the isolation filter plate (16) and one end of the liquid filling tube (15) are on the same plane.

7. The apparatus for separating fluid from frozen animal lymphatic tissue according to claim 6, characterized in that: The control component includes a ball valve (40), the inlet of which is connected to the outlet of the inlet pipe (39), the outlet of which is connected to the inlet of the cleaning nozzle (38), the ball valve (40) is fixedly mounted on the top of the separation box (11) by a support frame, a third friction wheel (41) is provided at one end of the valve stem of the ball valve (40), a friction plate (42) is provided on the third friction wheel (41), and the friction plate (42) is fixedly mounted on the upper surface of the fixed plate (14).

8. A frozen animal lymphatic tissue fluid separation device according to claim 6 or 7, characterized in that: The cleaning nozzle (38) is provided with a scraper (36) below it. One end of the scraper (36) is fixedly installed on the inner side wall of the separation box (11), and the lower surface of the scraper (36) is in close contact with the upper end surface of the liquid filling pipe (15).

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