Multi-cell climbing slide dyeing device and method

Abstract

The invention provides a multi-cell climbing slide dyeing device, and particularly relates to the technical field of cell culture and dyeing, the multi-cell climbing slide dyeing device comprises containing plates and a support, one side of the support is connected with a plurality of containing plates, the containing plates are horizontally arranged and parallel to one another, and the containing plates are connected with the support. The plurality of accommodating plates are arranged on one side of the bracket and are longitudinally arranged from one side of the bracket and are connected with the bracket, circular holes are formed in the accommodating plates, and a cell climbing slide is placed on each accommodating plate. The plurality of accommodating plates are used for placing a plurality of cell climbing slides, and the plurality of cell climbing slides are longitudinally arranged in the plate holes, so that the plurality of cell climbing slides can be dyed in the same dyeing solution; in addition, the staining solution can flow into each cell slide through the circular holes, so that the staining solution can be uniformly distributed, and the staining effect of each cell slide is consistent.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture and staining, and in particular to a multi-cell slide staining device. Background Art

[0002] In cell biology research, cell culture is one of the most common experimental techniques. It refers to extracting cells from animals or plants and then culturing them in a specific artificial environment for scientific research. It can be used to study cell physiology, biochemistry, potential mechanisms of disease, as well as drug screening and large-scale production of biological compounds, such as vaccines and therapeutic proteins.

[0003] Traditional staining methods usually rely on manual processing of single cell slides, which is cumbersome and prone to experimental errors. In addition, when processing multiple cell slides simultaneously in multiple holding plates, there is often uneven distribution of staining solution, inconsistent effects, and waste of antibodies.

[0004] In view of this, the present invention provides a multi-cell slide staining device and method that can ensure consistent staining effects while processing multiple cell slides. Summary of the invention

[0005] In order to solve the problem that multi-cell slides are prone to inconsistent staining effects, the present invention provides a multi-cell slide staining device and method.

[0006] The present invention is achieved through the following technical solutions:

[0007] The present invention provides a multi-cell slide staining device comprising a receiving plate and a bracket, wherein:

[0008] A plurality of accommodating plates are connected to one side of the support, the plurality of accommodating plates are horizontally arranged parallel to each other, and are longitudinally arranged and connected to the support from one side of the support, circular holes are arranged on the accommodating plates, and a cell climbing sheet is placed on each accommodating plate.

[0009] Furthermore, the spacings between adjacent accommodating plates are equal.

[0010] Furthermore, the spacing between adjacent accommodating plates is 0.25 mm.

[0011] Furthermore, the diameter of the circular hole is 6 mm.

[0012] Furthermore, the diameter of the containing plate is 24.1 mm.

[0013] Furthermore, a handle is included, and the accommodating plate is fixed to the bracket through the handle.

[0014] Furthermore, the bracket is printed from one or more of ABS plastic, PLA plastic or photosensitive resin.

[0015] Furthermore, a multi-cell slide staining method comprises the following steps:

[0016] S1. Place a cell slide on each receiving plate and place it in a well plate together with the support;

[0017] S2. Add an appropriate amount of staining solution to the well plate according to the number and height of the cell slides;

[0018] S3. Make the staining solution evenly distributed on each cell slide by oscillation and adsorption;

[0019] S4. After staining is completed, remove the cell slide from the receiving plate and conduct subsequent observation and analysis.

[0020] Beneficial effects of the present invention:

[0021] The multi-cell slide staining device proposed in the present invention utilizes multiple receiving plates to place multiple cell slides. The multiple cell slides are arranged longitudinally in the plate holes, and multiple cell slides can be stained with the same staining solution, reducing the waste of the staining solution. In addition, the staining solution can flow into each cell slide through the circular holes, thereby ensuring that the staining solution is evenly distributed, so that the staining effect of each cell slide is consistent. At the same time, when the staining solution is replaced, it is not easy to be contaminated by the staining solution. Finally, the problem of low efficiency in processing each cell slide individually can be avoided, thereby improving the experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an overall structural diagram of the multi-cell slide staining device of the present invention;

[0023] In the figure: a receiving plate 1, a circular hole 11, a bracket 2, and a handle 3;

[0024] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings in conjunction with the embodiments. DETAILED DESCRIPTION

[0025] In order to more clearly and completely illustrate the technical solution of the present invention, the present invention is further described below in conjunction with the accompanying drawings.

[0026] Please refer to Figure 1 The present invention provides a multi-cell slide staining device comprising a receiving plate 1 and a bracket 2, wherein:

[0027] A plurality of receiving plates 1 are connected to one side of the support 2. The plurality of receiving plates 1 are horizontally arranged parallel to each other and are longitudinally arranged and connected to the support 2 from one side of the support 2. A circular hole 11 is arranged on the receiving plate 1, and a cell climbing sheet is placed on each receiving plate 1.

[0028] In a specific embodiment, a plurality of receiving plates 1 are horizontally fixed on the side of the support 2 from top to bottom, and the number of receiving plates 1 can be selected according to actual conditions. The receiving plates 1 are used to place cell slides of different specifications, and the circular holes 11 on the receiving plates 1 can prevent liquid from remaining on the receiving plates 1. The support 2 and the receiving plates 1 can be disassembled and assembled so as to install different numbers of receiving plates 1 according to experimental requirements to place different numbers of cell slides. When staining, the entire support 2 is connected to the receiving plates 1 and placed in the well plate, and staining liquid is added until the receiving plates 1 are immersed. Since multiple cell slides are arranged longitudinally in the plate holes, multiple cell slides can be stained in the same staining liquid, reducing the waste of staining liquid. In addition, the staining liquid can flow into each cell slide through the circular holes 11, so that the staining liquid can be evenly distributed, so that the staining effect of each cell slide is consistent. At the same time, when the staining liquid is replaced, it is not easy to be contaminated by the staining liquid.

[0029] Furthermore, the spacing between adjacent accommodating plates 1 is equal, and the spacing between adjacent accommodating plates 1 is 0.25 mm.

[0030] In a specific embodiment, the spacing of the receiving plates 1 can also be selected according to the actual situation, so as to ensure that the cell climbing sheet is at a certain distance from the top receiving plate 1.

[0031] Furthermore, the diameter of the circular hole 11 is 6 mm, and the diameter of the receiving plate 1 is 24.1 mm.

[0032] In a specific embodiment, the circular hole 11 is used to discharge the staining solution, and the receiving plate 1 is used to receive the cell slide. The diameters of the receiving plate 1 and the circular hole 11 can also be selected according to the size of the cell slide.

[0033] Furthermore, a handle 3 is included, and the receiving plate 1 is fixed to the bracket 2 via the handle 3 .

[0034] In a specific implementation, the receiving plate 1 is fixed on the bracket 2 via the handle 3. When disassembling and installing, the handle 3 can be separated from or fixed to the bracket 2, which is more convenient during experiments.

[0035] Furthermore, the bracket 2 is printed from one or more of ABS plastic, PLA plastic or photosensitive resin.

[0036] In a specific embodiment, the bracket 2 needs to be selected to have good chemical resistance and stability to ensure that it does not react with the dyeing liquid during the dyeing process. The bracket 2 can be precisely printed using a 3D printer or other materials.

[0037] Furthermore, the multi-cell slide staining method comprises the following steps:

[0038] S1. Place a cell slide on each receiving plate 1 and place it in a well plate together with a support 2;

[0039] S2. Add an appropriate amount of staining solution to the well plate according to the number and height of the cell slides;

[0040] S3. Make the staining solution evenly distributed on each cell slide by oscillation and adsorption;

[0041] S4. After the staining is completed, the cell slide is removed from the receiving plate 1 and subsequent observation and analysis are performed.

[0042] In a specific embodiment, during dyeing, an oscillating device can also be used to help the dyeing liquid to be evenly distributed to improve the dyeing efficiency.

[0043] Of course, the present invention may have many other implementations. Based on this implementation, other implementations obtained by ordinary technicians in this field without any creative work are all within the scope of protection of the present invention.

Claims

1. A multi-cell slide staining device, characterized in that: It includes a receiving plate and a bracket, wherein: A plurality of accommodating plates are connected to one side of the support, the plurality of accommodating plates are horizontally arranged parallel to each other, and are longitudinally arranged and connected to the support from one side of the support, circular holes are arranged on the accommodating plates, and a cell climbing sheet is placed on each accommodating plate.

2. The multi-cell slide staining device according to claim 1, characterized in that: The distances between the adjacent receiving plates are equal.

3. The multi-cell slide staining device according to claim 2, characterized in that: The spacing between adjacent accommodating plates is 0.25 mm.

4. The multi-cell slide staining device according to claim 1, characterized in that: The diameter of the circular hole is 6 mm.

5. The multi-cell slide staining device according to claim 1, characterized in that: The diameter of the receiving plate is 24.1 mm.

6. The multi-cell slide staining device according to claim 1, characterized in that: A handle is also included, and the receiving plate is fixed to the bracket through the handle.

7. The multi-cell slide staining device according to claim 1, characterized in that: The bracket is printed from one or more of ABS plastic, PLA plastic or photosensitive resin.

8. A multi-cell slide staining method according to claim 1, using a multi-cell slide staining device according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Place a cell slide on each receiving plate and place it in a well plate together with the support; S2. Add an appropriate amount of staining solution to the well plate according to the number and height of the cell slides; S3. Make the staining solution evenly distributed on each cell slide by oscillation and adsorption; S4. After staining is completed, remove the cell slide from the receiving plate and conduct subsequent observation and analysis.

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