Water pollution source tracing detection device and detection method

Abstract

The present application belongs to the field of environmental monitoring and analysis technology, and specifically discloses a water pollution source tracing detection device and a detection method. The detection device includes: a microfluidic chip, a water sample inlet, a fluorescent auxiliary agent inlet and a mixed reaction channel are provided on the microfluidic chip, the water sample inlet and the fluorescent auxiliary agent inlet are respectively connected to the mixed reaction channel, and a mixed reaction water sample outlet is also provided at the outlet of the mixed reaction channel; a cuvette is connected to the mixed reaction water sample outlet; an optical detection unit is provided in the optical detection area of ​​the cuvette, so that the optical detection unit performs optical detection of the mixed reaction water sample in the cuvette. The present application can increase the detection sensitivity of the detection device by 2 to 3 times compared with the traditional technology, especially for the low response components in complex water samples, and can significantly improve its sensitivity by enhancing the fluorescence response of the low response components. At the same time, the present application can shorten the overall detection time by more than 20%, while reducing the cost of a single detection.

Description

Technical Field

[0001] The present application belongs to the field of environmental monitoring and analysis technology, and specifically relates to a water pollution source tracing detection device and detection method. Background Art

[0002] In the field of water quality testing, although traditional instrumental analysis methods (such as large-scale three-dimensional fluorescence spectrometers, liquid chromatography, and mass spectrometry) have high accuracy, they have the following disadvantages: First, they are highly dependent on large instruments. Traditional three-dimensional fluorescence spectrum detection instruments are usually desktop or larger equipment, which is not suitable for on-site emergency detection. Second, there is a lack of integrated micro-detection platforms. The existing technologies focus on single or simple component detection, which makes it difficult to deal with complex and multi-component pollution source samples. Third, the existing technologies are independent steps in sample pretreatment and analysis and detection, which leads to longer analysis time and consumes a lot of manpower and economic costs. Fourth, when facing complex backgrounds and multi-component mixtures, the existing technologies have insufficient detection sensitivity and selectivity, resulting in inaccurate results or failure to meet the requirements of fast and low detection limits. Therefore, the existing technologies have the disadvantages of being difficult to achieve on-site real-time rapid detection, lack of adaptability and universality for complex components, high cost, cumbersome operation, lack of portability, and lack of automation features. Summary of the invention

[0003] The present application aims to solve at least one of the technical problems in the related art to a certain extent. To this end, the purpose of the present application is to propose a water pollution source tracing detection device and detection method. The present application uses microfluidic chip technology to enable fluorescent additives to react efficiently with target substances in water samples, thereby increasing the detection sensitivity of the device by 2 to 3 times compared with traditional technologies. In particular, for low-response components in complex water samples, the sensitivity of low-response components can be significantly improved by enhancing the fluorescence response of low-response components, thereby providing more comprehensive detection results. At the same time, the present application can shorten the overall detection time by more than 20%, meet the needs of rapid detection, and further reduce the cost of a single detection through more efficient reagent utilization and detection processes.

[0004] In one aspect of the present application, the present application proposes a water pollution source tracing detection device. According to an embodiment of the present application, the water pollution source tracing detection device includes: A microfluidic chip, wherein a water sample inlet, a fluorescent additive inlet and a mixed reaction channel are provided on the microfluidic chip, wherein the water sample inlet and the fluorescent additive inlet are respectively connected to the mixed reaction channel, and a water sample outlet after the mixed reaction is also provided at the outlet of the mixed reaction channel; A cuvette, the cuvette being connected to an outlet of the water sample after the mixed reaction; An optical detection unit is arranged in the optical detection area of ​​the cuvette so that the optical detection unit can perform optical detection on the mixed reaction water sample in the cuvette.

[0005] According to the water pollution source tracing detection device of the embodiment of the present application, by setting a water sample inlet, a fluorescent auxiliary agent inlet and a mixed reaction channel on the microfluidic chip, the water sample to be detected can be effectively introduced into the mixed reaction channel through the water sample inlet, and the fluorescent auxiliary agent can be introduced into the mixed reaction channel through the fluorescent auxiliary agent inlet, so that the water sample to be detected and the fluorescent auxiliary agent are fully mixed and reacted in the mixed reaction channel, and the fluorescent auxiliary agent reacts efficiently with the target substance in the water sample (such as sewage), so that the detection sensitivity of the device is increased by 2 to 3 times compared with the traditional technology, especially for the low response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of the low response components can be significantly improved by enhancing the fluorescence response of the low response components, so as to provide more comprehensive detection results. These enhanced fluorescence signals are collected by the optical detection unit to form a complete three-dimensional fluorescence spectrum. Through the enhanced signals of specific wavelengths, pollutants with weak fluorescence responses, such as proteins, amino acids, etc., can be accurately identified. At the same time, fluorescent auxiliary agents can also detect more types of pollutants, especially low response substances that are difficult to detect with the prior art, effectively expanding the identification range of pollutants and improving the resolution and quantitative ability of complex pollutant samples.

[0006] This application uses microfluidic chip technology to effectively achieve miniaturized structure, high integration, fast reaction and analysis speed, and low reagent and sample usage. It can effectively realize integrated operations such as rapid sample pretreatment and detection, and shorten the overall detection time by more than 20%, meeting the needs of rapid detection. At the same time, through more efficient reagent utilization and detection processes, the cost of a single detection can be further reduced.

[0007] In addition, the water pollution source tracing detection device according to the above embodiment of the present application may also have the following additional technical features: In some embodiments of the present application, the microfluidic chip is further provided with multiple reagent inlets, and the multiple reagent inlets are connected to the mixing reaction channel.

[0008] In some embodiments of the present application, the water pollution source tracing detection device also includes: a microfluidic pump, which is respectively connected to the water sample inlet, the fluorescent additive inlet and the multiple reagent inlet; a first microvalve is arranged between the microfluidic pump and the water sample inlet, a second microvalve is arranged between the microfluidic pump and the fluorescent additive inlet, and a third microvalve is arranged between the microfluidic pump and the multiple reagent inlet.

[0009] In some embodiments of the present application, the mixing reaction channel is a serpentine channel, a tortuous channel or a multi-branch channel.

[0010] In some embodiments of the present application, the optical detection unit includes an excitation light source and a detection device, and the excitation light source and the detection device are respectively arranged in the optical detection area of ​​the cuvette.

[0011] In some embodiments of the present application, the excitation light source includes an LED light source; and / or the detection device includes at least one of a CCD detector and a CMOS detector.

[0012] In some embodiments of the present application, the optical detection unit also includes a filter group and a microlens, and the filter group and the microlens are arranged between the excitation light source and the cuvette; the optical detection unit also includes a microprism, and the microprism is arranged between the detection device and the cuvette.

[0013] In the second aspect of the present application, the present application proposes a method for water pollution source tracing detection using the water pollution source tracing detection device described in the above embodiment. According to the embodiment of the present application, the method includes: (1) introducing a water sample to be tested into the mixed reaction channel through a water sample inlet, and simultaneously introducing a fluorescent auxiliary agent into the mixed reaction channel through a fluorescent auxiliary agent inlet; (2) The water sample to be detected and the fluorescent auxiliary agent are mixed and reacted in the mixing reaction channel; (3) The water sample after the mixed reaction enters the cuvette through the water sample outlet after the mixed reaction; (4) The mixed reaction water sample in the cuvette is optically detected by an optical detection unit.

[0014] According to the method for tracing the source of water pollution in the embodiment of the present application, the water sample to be detected is introduced into the mixed reaction channel through the water sample inlet, and the fluorescent auxiliary agent is introduced into the mixed reaction channel through the fluorescent auxiliary agent inlet, so that the water sample to be detected and the fluorescent auxiliary agent are fully mixed and reacted in the mixed reaction channel, and the fluorescent auxiliary agent reacts efficiently with the target substance in the water sample (such as sewage), so that the detection sensitivity is increased by 2 to 3 times compared with the traditional technology, especially for the low response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of the low response components can be significantly improved by enhancing the fluorescence response of the low response components, so as to provide more comprehensive detection results. These enhanced fluorescence signals are collected by the optical detection unit to form a complete three-dimensional fluorescence spectrum. Through the enhanced signals of specific wavelengths, pollutants with weaker fluorescence responses, such as proteins, amino acids, etc., can be accurately identified. At the same time, fluorescent auxiliary agents can also detect more types of pollutants, especially low-response substances that are difficult to detect with existing technologies, effectively expanding the identification range of pollutants and improving the resolution and quantitative ability of complex pollutant samples.

[0015] In addition, this method can effectively realize integrated operations such as rapid pretreatment and detection of samples, shortening the overall detection time by more than 20%, meeting the needs of rapid detection, and further reducing the cost of a single test through more efficient reagent utilization and detection processes.

[0016] In addition, the method for tracing the source of water pollution according to the above embodiment of the present application may also have the following additional technical features: In some embodiments of the present application, the fluorescent auxiliary agent includes at least one of rhodamine and fluorescein isothiocyanate.

[0017] In some embodiments of the present application, step (1) further includes: introducing multiple reagents into the mixed reaction channel through multiple reagent inlets, wherein the multiple reagents include at least one of a pH regulator, an anti-quenching agent, a stabilizer and a dispersant.

[0018] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which: Figure 1 A top view of a water pollution source tracing detection device according to an embodiment of the present application; Figure 2 It is a cross-sectional schematic diagram of a water pollution source tracing detection device according to an embodiment of the present application.

[0020] Reference numerals: 100-microfluidic chip, 101-mixing reaction channel, 102-water sample inlet, 103-fluorescent auxiliary agent inlet, 104-multiple reagent inlet, 200-cuvette, 300-optical detection unit, 301-excitation light source, 302-detection device, 303-microprism, 400-microfluidic pump, 501-first microvalve, 502-second microvalve, 503-third microvalve. DETAILED DESCRIPTION

[0021] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

[0022] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred elements must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present application.

[0023] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0024] In this application, unless otherwise clearly specified and limited, the terms such as "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0025] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0026] In one aspect of the present application, the present application proposes a water pollution source tracing detection device. According to the embodiment of the present application, refer to the attached Figure 1-2The above-mentioned water pollution source tracing detection device includes: a microfluidic chip 100, on which a water sample inlet 102, a fluorescent auxiliary agent inlet 103 and a mixed reaction channel 101 are arranged, the water sample inlet 102 and the fluorescent auxiliary agent inlet 103 are respectively connected to the mixed reaction channel 101, and a mixed reaction water sample outlet is also arranged at the outlet of the mixed reaction channel 101; a cuvette 200, which is connected to the mixed reaction water sample outlet; an optical detection unit 300, which is arranged in an optical detection area of ​​the cuvette 200, so that the optical detection unit 300 can perform optical detection on the mixed reaction water sample in the cuvette 200.

[0027] The beneficial effects that can be achieved by the water pollution source tracing detection device proposed in this application are described in detail below: The existing three-dimensional fluorescence spectrum detection technology mainly targets fluorescent response substances, and has insufficient detection capabilities for pollutants with weak fluorescence response. To solve this problem, the present application sets a water sample inlet 102, a fluorescent auxiliary agent inlet 103 and a mixed reaction channel 101 on the microfluidic chip 100, and can effectively introduce the water sample to be detected into the mixed reaction channel 101 through the water sample inlet 102, and at the same time introduce the fluorescent auxiliary agent into the mixed reaction channel 101 through the fluorescent auxiliary agent inlet 103, so that the water sample to be detected and the fluorescent auxiliary agent are fully mixed and reacted in the mixed reaction channel 101, and through the efficient reaction of the fluorescent auxiliary agent with the target substance in the water sample (such as sewage), the detection sensitivity of the device is improved by 2 to 3 times compared with the traditional technology, especially for low-response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of the low-response components can be significantly improved by enhancing the fluorescence response of the low-response components, thereby providing more comprehensive detection results. These enhanced fluorescence signals are collected by the optical detection unit 300 to form a complete three-dimensional fluorescence spectrum. Through the enhanced signals of specific wavelengths, pollutants with weak fluorescence responses, such as proteins, amino acids, etc., can be accurately identified. At the same time, the fluorescent additive can also detect more types of pollutants, especially low-response substances that are difficult to detect with existing technologies, effectively expanding the identification range of pollutants and improving the resolution and quantitative capabilities of complex pollutant samples.

[0028] This application uses microfluidic chip technology to effectively achieve miniaturized structure, high integration, fast reaction and analysis speed, and low reagent and sample usage. It can effectively realize integrated operations such as rapid sample pretreatment and detection, and shorten the overall detection time by more than 20%, meeting the needs of rapid detection. At the same time, through more efficient reagent utilization and detection processes, the cost of a single detection can be further reduced.

[0029] The present application combines microfluidic chip technology with optical detection methods, wherein the microfluidic chip 100 can complete the pretreatment (i.e., mixing reaction) and transportation of water samples in a micron-scale channel, and the three-dimensional fluorescence detection can quickly capture the characteristic fluorescence information of each component in the water sample, thereby realizing rapid qualitative and quantitative analysis.

[0030] In the embodiments of the present application, the specific types of the above-mentioned fluorescent additives are not particularly limited. According to some specific embodiments of the present application, the above-mentioned fluorescent additives include at least one of the fluorescent markers such as rhodamine and fluorescein isothiocyanate. The above-mentioned types of fluorescent additives can react efficiently with the target substance in the water sample, thereby increasing the detection sensitivity of the device by 2 to 3 times compared with the traditional technology. In particular, for low-response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of low-response components can be significantly improved by enhancing the fluorescence response of low-response components, thereby providing more comprehensive detection results. The above-mentioned fluorescent additives can react quickly with specific functional groups (such as amine or carboxyl groups) in the target pollutants to generate products with high fluorescent signals. The above-mentioned fluorescent additives not only improve the detection sensitivity, but also expand the range of compatible substances to meet the needs of complex water sample analysis.

[0031] In the embodiment of the present application, the microfluidic chip 100 is micrometer-scale, and the width of the microfluidic chip 100 is 100 micrometers to 500 micrometers. The microfluidic chip 100 can be made of polymer or glass material, preferably polymer (such as polydimethylsiloxane, polymethyl methacrylate, etc.).

[0032] According to some specific embodiments of the present application, Figure 1 The microfluidic chip 100 is also provided with a multiple reagent inlet 104, which is connected to the mixed reaction channel 101. The multiple reagents can be introduced into the mixed reaction channel 101 through the multiple reagent inlet 104. The multiple reagents include at least one of a pH regulator, an anti-quenching agent, a stabilizer (such as ascorbic acid, etc.) and a dispersant, thereby optimizing the detection conditions and improving the fluorescence signal. Among them, the anti-quenching agent can effectively reduce the fluorescence quenching of the fluorescent auxiliary agent due to the interaction with the surrounding environment (such as oxygen, heavy metal ions, halide ions, etc.), thereby improving the fluorescence signal.

[0033] According to some further specific embodiments of the present application, refer to the attached Figure 1The water pollution source tracing detection device further includes: a microfluidic pump 400, which is connected to the water sample inlet 102, the fluorescent auxiliary agent inlet 103 and the multiple reagent inlet 104 respectively; a first microvalve 501 is arranged between the microfluidic pump 400 and the water sample inlet 102, a second microvalve 502 is arranged between the microfluidic pump 400 and the fluorescent auxiliary agent inlet 103, and a third microvalve 503 is arranged between the microfluidic pump 400 and the multiple reagent inlet 104. The main function of the microfluidic pump 400 is to promote the water sample to flow in the microfluidic chip 100. It can provide a stable water sample pressure to ensure that the water sample flows along a predetermined path; at the same time, the microfluidic pump 400 can accurately control the flow rate of the water sample, which is crucial to ensure that the reactants are fully mixed in the mixing reaction channel 101 and accurately measured in the optical detection area; in addition, by adjusting the flow rate of the microfluidic pump 400, the residence time of the water sample in the mixing reaction channel 101 can be controlled, thereby affecting the speed and efficiency of the reaction. In a multi-channel system, the microfluidic pump 400 can also be used to switch between different fluids to achieve precise mixing of multi-component fluids. The first microvalve 501 is used to control the on-off of the water sample, the second microvalve 502 is used to control the on-off of the fluorescent auxiliary agent, and the third microvalve 503 is used to control the on-off of multiple reagents. Thus, the microfluidic pump 400, the first microvalve 501, the second microvalve 502 and the third microvalve 503 can effectively achieve precise adjustment of the sample flow rate, flow rate and reaction time through the joint action, providing a reliable guarantee for the stable capture of the fluorescent signal.

[0034] In order to solve the problem of insufficient mixing efficiency of the fluorescent additive and the water sample, the present application optimizes the three-dimensional structure of the mixing reaction channel 101. Figure 1 The above-mentioned mixed reaction channel 101 is a serpentine channel, a zigzag channel or a multi-branch channel, thereby increasing the mixing path and turbulence effect of the fluid, further improving the mixing efficiency and reaction rate, and ensuring that the fluorescent additive and the water sample fully react in a short time. Simulation optimization shows that this three-dimensional mixing structure significantly improves the reaction uniformity and shortens the reaction time to 50% of the traditional design. At the same time, by adjusting the length and flow rate of the mixed reaction channel 101, the fluorescence reaction time can be seamlessly connected with the detection process.

[0035] According to some further specific embodiments of the present application, refer to the attached Figure 2The optical detection unit 300 is a micro optical detection unit 300. The optical detection unit 300 includes an excitation light source 301 and a detection device 302. The excitation light source 301 and the detection device 302 are respectively arranged in the optical detection area of ​​the cuvette 200. The excitation light source 301 (such as a high-power wide-spectrum LED) provides light of a specific wavelength to excite the fluorescent substance in the water sample to be detected. When the fluorescent substance absorbs these photons, it will enter an excited state and emit fluorescence when returning to the ground state. By integrating a high-power wide-spectrum LED, it can work under multiple excitation wavelength conditions to generate a three-dimensional fluorescence spectrum of the sample, which helps to improve the recognition ability of different fluorescent substances. The detection device 302 is used to collect the fluorescence signals emitted by the sample. These signals contain the fluorescence characteristic information of the water sample and can be used for subsequent analysis and identification. The detection device 302 can record the emission spectrum under multiple excitation wavelength conditions and finally generate a three-dimensional fluorescence spectrum of the sample.

[0036] 3D Fluorescence Spectrum: The emission spectrum information generated by the sample at multiple excitation wavelengths is comprehensively presented to form a three-dimensional data matrix consisting of excitation wavelength, emission wavelength and fluorescence intensity. This spectrum can provide unique optical "fingerprints" for different components in the sample and can be used for qualitative identification and quantitative analysis of complex mixtures.

[0037] In the embodiments of the present application, the specific type of the excitation light source 301 is not particularly limited. As some specific embodiments, the excitation light source 301 includes an LED light source or a laser diode, preferably an LED light source.

[0038] In the embodiments of the present application, the specific type of the above-mentioned detection device 302 is not particularly limited. As some specific embodiments, the detection device 302 includes at least one of a high-sensitivity CCD detector and a CMOS detector, which can capture weak fluorescence response signals under complex backgrounds, thereby greatly improving the sensitivity and resolution of detection.

[0039] According to some other specific embodiments of the present application, refer to the attached Figure 2The optical detection unit 300 also includes a filter set and a microlens, which are arranged between the excitation light source 301 and the cuvette 200; the optical detection unit 300 also includes a microprism 303, which is arranged between the detection device 302 and the cuvette 200. The excitation light source 301 is coupled into the cuvette 200 through the microlens to ensure that the excitation light can be effectively irradiated onto the water sample, thereby improving the intensity of the fluorescent signal and the detection sensitivity. The use of optical elements such as the microprism 303 can effectively improve the signal collection efficiency of the detection device 302, reduce scattering and background interference, and thus improve the accuracy and reliability of the detection. The filter set can achieve efficient signal enhancement while suppressing background noise.

[0040] According to some other specific embodiments of the present application, the above-mentioned water pollution source tracing detection device also includes: an algorithm and software support system, which can deconstruct the three-dimensional fluorescence data based on a multidimensional data processing algorithm (such as parallel factor analysis PARAFAC, principal component analysis PCA) to achieve rapid identification and quantitative analysis of target pollutants. Combined with enhanced fluorescence spectrum data, the partitioning algorithm is optimized to improve the pollutant identification rate.

[0041] In the second aspect of the present application, the present application proposes a method for water pollution source tracing detection using the water pollution source tracing detection device of the above embodiment. According to the embodiment of the present application, the above method includes: (1) The water sample to be tested is introduced into the mixed reaction channel through the water sample inlet, and the fluorescent auxiliary agent is introduced into the mixed reaction channel through the fluorescent auxiliary agent inlet.

[0042] In the embodiments of the present application, the specific types of the above-mentioned fluorescent additives are not particularly limited. According to some specific embodiments of the present application, the above-mentioned fluorescent additives include at least one of the fluorescent markers such as rhodamine and fluorescein isothiocyanate. The above-mentioned types of fluorescent additives can react efficiently with the target substance in the water sample, thereby increasing the detection sensitivity of the device by 2 to 3 times compared with the traditional technology. In particular, for low-response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of low-response components can be significantly improved by enhancing the fluorescence response of low-response components, thereby providing more comprehensive detection results. The above-mentioned fluorescent additives can react quickly with specific functional groups (such as amine or carboxyl groups) in the target pollutants to generate products with high fluorescent signals. The above-mentioned fluorescent additives not only improve the detection sensitivity, but also expand the range of compatible substances to meet the needs of complex water sample analysis.

[0043] According to some specific embodiments of the present application, step S100 further includes: introducing multiple reagents into the mixed reaction channel through the multiple reagent inlet, the multiple reagents including at least one of a pH regulator, an anti-fading agent, a stabilizer (such as ascorbic acid, etc.) and a dispersant, thereby optimizing the detection conditions and improving the fluorescence signal. Among them, the anti-fading agent can effectively reduce the fluorescence quenching of the fluorescent auxiliary agent due to the interaction with the surrounding environment (such as oxygen, heavy metal ions, halide ions, etc.), thereby improving the fluorescence signal.

[0044] (2) The water sample to be tested and the fluorescent additive are mixed and reacted in the mixing reaction channel.

[0045] In this step, the water sample to be tested and the fluorescent additive are mixed and reacted in the mixed reaction channel. Through the efficient reaction of the fluorescent additive with the target substance in the water sample, the detection sensitivity of the device is increased by 2 to 3 times compared with the traditional technology. Especially for low-response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of low-response components can be significantly improved by enhancing the fluorescence response of low-response components, thereby providing more comprehensive detection results. These enhanced fluorescence signals are collected by the optical detection unit to form a complete three-dimensional fluorescence spectrum. Through the enhanced signals of specific wavelengths, pollutants with weaker fluorescence responses, such as proteins, amino acids, etc., can be accurately identified. At the same time, fluorescent additives can also detect more types of pollutants, especially low-response substances that are difficult to detect with existing technologies, effectively expanding the identification range of pollutants and improving the resolution and quantitative capabilities of complex pollutant samples.

[0046] (3) After the mixing reaction, the water sample enters the cuvette through the water sample outlet after the mixing reaction.

[0047] (4) After the mixed reaction in the cuvette, the water sample is optically detected by the optical detection unit.

[0048] In this step, the mixed reaction water sample in the cuvette is optically detected by the optical detection unit. Specifically, by integrating high-power wide-spectrum LED, it can work under multiple excitation wavelength conditions to generate a three-dimensional fluorescence spectrum of the sample. The detection device is used to collect the fluorescence signals emitted by the sample. These signals contain the fluorescence characteristic information of the water sample and can be used for subsequent analysis and identification. Through enhanced detection in a specific wavelength range, pollutants with weak fluorescence response, such as proteins and amino acids, can be accurately identified.

[0049] According to some further specific embodiments of the present application, the above method further includes: Based on multidimensional data processing algorithms (such as parallel factor analysis PARAFAC and principal component analysis PCA), the three-dimensional fluorescence data are deconstructed to achieve rapid identification and quantitative analysis of target pollutants.

[0050] According to the method for tracing the source of water pollution in the embodiment of the present application, the water sample to be detected is introduced into the mixed reaction channel through the water sample inlet, and the fluorescent auxiliary agent is introduced into the mixed reaction channel through the fluorescent auxiliary agent inlet, so that the water sample to be detected and the fluorescent auxiliary agent are fully mixed and reacted in the mixed reaction channel, and the fluorescent auxiliary agent reacts efficiently with the target substance in the water sample (such as sewage), so that the detection sensitivity is increased by 2 to 3 times compared with the traditional technology, especially for the low response components (such as proteins, amino acids, etc.) in complex water samples, the sensitivity of the low response components can be significantly improved by enhancing the fluorescence response of the low response components, so as to provide more comprehensive detection results. These enhanced fluorescence signals are collected by the optical detection unit to form a complete three-dimensional fluorescence spectrum. Through the enhanced signals of specific wavelengths, pollutants with weaker fluorescence responses, such as proteins, amino acids, etc., can be accurately identified. At the same time, fluorescent auxiliary agents can also detect more types of pollutants, especially low-response substances that are difficult to detect with existing technologies, effectively expanding the identification range of pollutants and improving the resolution and quantitative ability of complex pollutant samples.

[0051] In addition, this method can effectively realize integrated operations such as rapid pretreatment and detection of samples, shortening the overall detection time by more than 20%, meeting the needs of rapid detection, and further reducing the cost of a single test through more efficient reagent utilization and detection processes.

[0052] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0053] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A water pollution source tracing detection device, characterized in that: include: A microfluidic chip, wherein a water sample inlet, a fluorescent additive inlet and a mixed reaction channel are provided on the microfluidic chip, wherein the water sample inlet and the fluorescent additive inlet are respectively connected to the mixed reaction channel, and a water sample outlet after the mixed reaction is also provided at the outlet of the mixed reaction channel; A cuvette, the cuvette being connected to an outlet of the water sample after the mixed reaction; An optical detection unit is arranged in the optical detection area of ​​the cuvette so that the optical detection unit can perform optical detection on the mixed reaction water sample in the cuvette.

2. The water pollution source tracing detection device according to claim 1 is characterized in that: The microfluidic chip is also provided with multiple reagent inlets, and the multiple reagent inlets are connected with the mixed reaction channel.

3. The water pollution source tracing detection device according to claim 2 is characterized in that: Also includes: A microfluidic pump, wherein the microfluidic pump is respectively connected to the water sample inlet, the fluorescent auxiliary agent inlet and the multiple reagent inlet; A first microvalve is arranged between the microfluidic pump and the water sample inlet, a second microvalve is arranged between the microfluidic pump and the fluorescent auxiliary agent inlet, and a third microvalve is arranged between the microfluidic pump and the multiple reagent inlet.

4. The water pollution source tracing detection device according to claim 1 is characterized in that: The mixing reaction channel is a serpentine channel, a tortuous channel or a multi-branch channel.

5. The water pollution source tracing detection device according to any one of claims 1 to 4, characterized in that: The optical detection unit includes an excitation light source and a detection device, and the excitation light source and the detection device are respectively arranged in the optical detection area of ​​the cuvette.

6. The water pollution source tracing detection device according to claim 5 is characterized in that: The excitation light source includes an LED light source; And / or, the detection device includes at least one of a CCD detector and a CMOS detector.

7. The water pollution source tracing detection device according to claim 5 is characterized in that: The optical detection unit further comprises a filter set and a microlens, wherein the filter set and the microlens are arranged between the excitation light source and the cuvette; The optical detection unit further includes a microprism, and the microprism is disposed between the detection device and the cuvette.

8. A method for water pollution source tracing detection using the water pollution source tracing detection device according to any one of claims 1 to 7, characterized in that: include: (1) introducing a water sample to be tested into the mixed reaction channel through a water sample inlet, and simultaneously introducing a fluorescent auxiliary agent into the mixed reaction channel through a fluorescent auxiliary agent inlet; (2) The water sample to be detected and the fluorescent auxiliary agent are mixed and reacted in the mixing reaction channel; (3) The water sample after the mixed reaction enters the cuvette through the water sample outlet after the mixed reaction; (4) The mixed reaction water sample in the cuvette is optically detected by an optical detection unit.

9. The method according to claim 8, characterized in that The fluorescent auxiliary agent includes at least one of rhodamine and fluorescein isothiocyanate.

10. The method according to claim 8, characterized in that Step (1) also includes: introducing multiple reagents into the mixed reaction channel through multiple reagent inlets, wherein the multiple reagents include at least one of a pH regulator, an anti-quenching agent, a stabilizer and a dispersant.

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