Method for introducing lycium barbarum in northern facility salinized soil
By introducing wolfberry vegetables in salinized soil in northern facilities and applying microbial fertilizers such as Trichoderma fertilizer, the problem of secondary salinization of vegetable soil in facility has been solved, the planting efficiency and yield have been improved, and the fertilizer investment and pollution risks have been reduced.
Patent Information
- Application Number
- CN202510069687.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-16
- Publication Date
- 2025-04-11
AI Technical Summary
In the cultivation of vegetable plants in northern facilities, the secondary salinization of soil is serious, affecting yield and quality, and the existing prevention and control methods are costly and there is a risk of secondary pollution.
A method of introducing wolfberry vegetables in salinized soil in northern facilities is adopted. Through the autumn cutting of wolfberry seedlings, transplanting them to the soil of the facility, and applying microbial fertilizers such as Trichoderma fertilizer to reduce the investment in chemical fertilizers and promote the growth of wolfberry vegetables in salinized soil.
It improves the planting efficiency of salinized soil in the facility, reduces the cost of fertilizer investment, reduces the entry of salt from the source, avoids secondary soil pollution, and significantly increases the photosynthetic rate and leafy vegetable yield of wolfberry vegetables.
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Figure CN119790845A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of facility vegetable cultivation, and more particularly to a method for introducing Chinese wolfberry vegetables into salinized soil in northern facilities. Background Art
[0002] Facility cultivation is a general term for horticultural facility cultivation such as glass greenhouses and plastic greenhouses. Compared with open-field vegetables, facility vegetable cultivation can achieve higher yields and economic benefits. The sown area of facility vegetables in my country reached 5.48 million hm2 in 2016. 2 , with a total output of about 280 million tons. Facility vegetable fields are in a confined space, lacking rainwater for washing, and artificial irrigation is limited to the plough layer. The ground evaporation is strong. As the years of facility cultivation increase, secondary salinization of the soil is very likely to occur. Excessive application of chemical fertilizers in pursuit of high yields, which destroys the soil structure and aggravates the accumulation of salt in the plough layer, is the main cause of secondary salinization of facility vegetable soil. At present, one of the biggest problems facing facility vegetable cultivation in my country is secondary salinization of the soil. Shandong Province is an important facility vegetable production base and one of the main production areas in northern my country. Due to large fertilizer input, increased years of facility cultivation and improper management, about 39.73% of the facility soil in Shandong Province has different degrees of salinization. Conventional vegetables such as tomatoes, cucumbers, and peppers are the main crops grown in facility vegetable fields in northern provinces such as Shandong. They are very sensitive to salt stress. Soil salinization seriously affects their yield and quality, and even causes plant death, greatly reducing the planting benefits.
[0003] At present, the prevention and control of soil salinization in facilities is mostly focused on the research and development and application of soil conditioners. The patent "A special improver for secondary salinization of facility soil and its preparation method" (application number: 201010238662.4) provides a soil conditioner and preparation method using Chinese medicine residue, livestock and poultry manure, slag, and bacterial agents as raw materials. The patent "A soil conditioner for facilities and its application" (application number: 201910110162.3) provides a soil conditioner prepared with biochar, pig manure, kiln ash potash fertilizer and oil shale ash as raw materials. When applied to the surface soil, it can reduce salt, increase nutrients and improve soil structure. The patent "A biologically active conditioner for improving salinization of facility soil and its application" (application number: 201910110162.3) provides a biologically active soil conditioner mixed with biochar, Trichoderma agents, Bacillus agents and organic fertilizers. When applied to the surface soil, it can reduce soil salt and increase vegetable yields. The patent "A method for preparing and using a fertilizer to prevent salinization of facility soil" (application number: 201310232229.3) provides a fertilizer and preparation method prepared with high-tower urea-sulfur-based return materials, diammonium phosphate, humic acid and digestion inhibitors as raw materials, which can play a role in reducing soil salinity. The patent "A field regulation method for improving facility salinized soil" (application number: 201410192576.2) comprehensively applies soil structure conditioners, soil water retention agents, crop straw and EM bacteria to reduce soil salinity and improve soil nutrients. Although the application of soil conditioners can improve facility salinized soil, it increases input costs and has the risk of causing secondary soil pollution. It also does not consider the application of chemical fertilizers, and cannot reduce the entry of salt from the source. "A method for alkalizing facility soil by controlled irrigation and crop rotation" (application number: 201910420633.0) Install soil moisture monitors to detect soil moisture in real time, determine irrigation volume, and adopt crop rotation cultivation methods such as corn and celery to reduce soil salinity and increase crop yields. "A comprehensive treatment method for continuously cropped saline facility soil" (application number: 201310232229.3) Comprehensively use film mulching cultivation, freshwater flushing ditches, high-temperature fumigation of topsoil, and topsoil stacking to improve saline soil. Although the above two methods do not require additional application of modifiers, they require equipment installation or high-temperature steam, which further increases the input cost. "A fertilization method to alleviate secondary salinization of facility soil" (application number: 201811338138.7) Use organic fertilizers to replace chemical fertilizers to reduce soil salinity and increase pepper yields. Compared with organic fertilizer, microbial fertilizer not only contains organic matter, but also is rich in beneficial growth-promoting bacteria. Therefore, the use of microbial fertilizer to replace chemical fertilizer has potential advantages, but there are no relevant patent reports. Introducing salt-tolerant vegetables with high economic value to adapt to the facility soil environment with increased salt content instead of using high-cost soil salt reduction methods is an effective way to increase the benefits of facility salinized soil cultivation, but it has not yet received attention.
[0004] The young leaves of Lycium barbarum, also known as Lycium barbarum, are a kind of health-care vegetable that can be used as both food and medicine. The sales profit is much higher than that of conventional vegetables such as tomatoes and cucumbers. Lycium barbarum is a perennial halophyte, mainly cultivated in southern regions such as Zhejiang and Guangdong. There are no reports on the introduction of Lycium barbarum into vegetable fields in the north.
[0005] Therefore, how to develop a method for growing Chinese wolfberry in salinized soil in northern facilities is a technical problem that technicians in this field need to solve urgently. Summary of the invention
[0006] In view of this, the present invention provides a method for introducing Chinese wolfberry leaves into salinized soil in northern facilities.
[0007] In order to achieve the above object, the present invention adopts the following technical solution:
[0008] A method for introducing Chinese wolfberry leaves into salinized soil in northern facilities, comprising the following steps:
[0009] (1) In autumn, cut the current year's shoots with a length of 10-15 cm and a diameter of 0.5-0.8 cm from healthy wolfberry plants, and plant them in a seedling tray filled with a substrate, or directly plant them in local soil. Keep the substrate or soil moist until capillary roots grow at the bottom of the shoots one month later, and set aside;
[0010] (2) In November and December, dig out the wolfberry seedlings with the substrate or soil and transplant them into the facility soil. The salt content of the facility soil is less than 0.4%. Cultivate them on flat beds with a row spacing of 20 cm. Water them thoroughly immediately after transplanting. During the growing season, irrigate once a month in winter, 1-2 times a month in spring and autumn, and 3-4 times a month in summer. The irrigation method is bed irrigation or drip irrigation, and the irrigation volume is 0.10-0.12m 3 / m 2 ;
[0011] (3) The first crop of leafy vegetables was harvested 60 days after planting, and then the next crop of leafy vegetables was harvested every 25-30 days;
[0012] (4) Ensure ventilation in the facility, harvest tender branches and leaves in a timely manner, and spray new branches and leaves with pesticides during the non-harvest period;
[0013] (5) In January, Trichoderma fertilizer was applied together with urea, calcium magnesium phosphate fertilizer and potassium sulfate as basal fertilizer. Urea and potassium sulfate were applied every two months. The basal fertilizer was turned into the soil, and the topdressing fertilizer was turned into the soil or applied with drip irrigation using water-soluble fertilizer. The application rate of Trichoderma fertilizer was 3.55 kg / m 2 The application amount of calcium magnesium phosphate fertilizer is 735.84g / m 2 The base application rate and topdressing rate of urea were 36.5 g / m 2 The base application rate and topdressing rate of potassium sulfate were 32.4 g / m2 The annual application rates of total nitrogen, total phosphorus and total potassium are 240kg / ha, 160kg / ha and 240kg / ha respectively.
[0014] Further, in step (5), the preparation method of Trichoderma fertilizer comprises the following steps:
[0015] 1) The apple pectin residue after pectin extraction is first subjected to natural composting for 20-24 days to obtain organic compost;
[0016] 2) preparing a Trichoderma liquid from the Trichoderma acanthocereus strain by a shake flask method;
[0017] 3) spraying the Trichoderma liquid evenly onto the spread organic compost obtained in step 1), wherein the inoculation amount of the Trichoderma liquid is controlled so that the volume mass ratio of the Trichoderma liquid to the organic compost is (5-20) L / 100 kg, and then stacking the materials into a trapezoidal pile shape, turning the pile every 3 days at room temperature, completing the Trichoderma fermentation in 7 to 14 days, spreading and airing the pile until the moisture content is 30%, and crushing the pile to obtain the Trichoderma fertilizer.
[0018] The beneficial effect of adopting the above further technical solution is that the apple pomace is used as a fermentation material to prepare Trichoderma fertilizer, which is beneficial to promote the resource utilization of pomace waste, save costs, and eliminate environmental pollution hazards.
[0019] Further, in step 2), the method of preparing Trichoderma liquid by shaking flask method using Trichoderma spp. strain comprises the following steps: picking hyphae at the edge of potato dextrose agar medium plate or scraping green spores, transferring hyphae or spores into potato dextrose liquid medium, and culturing at 28°C with a rotation speed of 150 rpm for 4-5 days to form a large amount of white flocculent hyphae and obtain Trichoderma liquid.
[0020] Further, the preparation of potato glucose agar medium: weigh 15g potato extract powder, add 1000mL water, add 20g glucose and 18g agar, sterilize at 121°C for 20 minutes, divide into culture dishes, cool and store for later use; preparation of potato glucose culture solution: weigh 15g potato extract powder, add 1000mL water, add 20g glucose, sterilize at 121°C for 20 minutes, cool and store for later use.
[0021] Furthermore, in step (1), the raw materials of the substrate are peat, perlite, peat and coconut husk, the organic matter content is ≥45%, and the pH is neutral.
[0022] Furthermore, the specific steps of step (4) are as follows: ventilate the facility every day except on rainy days, ventilate once a week in spring and autumn, ventilate during daytime, ventilate for 8-12 hours a day, harvest tender branches and leaves in time, spray pesticides for prevention before new branches and leaves sprout and after leaf unfolding in the non-harvest period, spray 0.5 degrees Baume lime sulfur mixture to control powdery mildew and wolfberry gall mites, spray 500 times diluted 50% dimethoate to control psyllids and aphids, and spray 1000 times diluted 20% dicofol emulsifiable concentrate to control mites.
[0023] The beneficial effects of adopting the above further technical solution are as follows: ventilation helps to reduce air humidity, avoid pests and diseases, and spraying pesticides can prevent pests and diseases in advance.
[0024] The beneficial effects of the present invention are as follows: (1) Based on the principle of plant adaptability to adverse stress, the halophyte Lycium barbarum with high economic value is introduced, and high-cost soil salt reduction methods are not used, thereby improving the planting efficiency of facility salinized soil.
[0025] (2) Establish technology to partially replace chemical fertilizers with microbial fertilizers to promote the growth of wolfberry leaves in salinized soils in facilities, reduce the cost of chemical fertilizer input, and reduce the entry of salt from the source.
[0026] Digging out the wolfberry seedlings with substrate or soil can prevent root damage. Immediately water after transplanting to promote the survival of the seedlings. Wolfberry is a halophyte of the genus Lycium and has a high salt tolerance threshold. The salt content of the soil in facilities with secondary salinization in northern areas such as Shandong is mostly below 0.4%, which has little effect on the normal growth of wolfberry. During the growing season, irrigate once a month in winter and 1-2 times a month in spring and autumn. In summer, the evaporation is large, and the irrigation frequency increases accordingly depending on the soil moisture conditions. The irrigation amount is 0.10-0.12m each time. 3 / m 2 , which can keep the soil moisture in good condition and avoid drought and waterlogging. Lycium barbarum is a perennial small shrub. It can be planted once and harvested continuously for many years. The main diseases and pests of Lycium barbarum are powdery mildew and aphids. Prevention is the main measure. The facility should be ventilated and the young branches and leaves should be harvested in time to avoid diseases and pests on old leaves. Excessive application of chemical fertilizers is the main cause of secondary salinization of vegetable soil in facilities. The present invention establishes a scheme for partially replacing chemical fertilizers with the application of microbial fertilizers. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 Experimental plot of vegetable land with secondary salinization in facilities;
[0028] Figure 2 Effects of Trichoderma fertilizer on photosynthetic rate of wolfberry. CK, control plot without nitrogen fertilizer; 100% CF, plot with full fertilizer application; OF+70% CF, plot with organic fertilizer application and 30% chemical fertilizer reduction; TF+70% CF, plot with Trichoderma fertilizer application and 30% chemical fertilizer reduction;
[0029] Figure 3 Effect of Trichoderma fertilizer on the fresh weight of wolfberry leaves. CK, control plot without nitrogen fertilizer; 100% CF, plot with full fertilizer application; OF+70% CF, plot with organic fertilizer application and 30% chemical fertilizer reduction; TF+70% CF, plot with Trichoderma fertilizer application and 30% chemical fertilizer reduction;
[0030] Figure 4 Effects of Trichoderma fertilizer on soil mineral nitrogen content. CK, control plot without nitrogen fertilizer; 100% CF, plot with full fertilizer application; OF+70% CF, plot with organic fertilizer application and 30% chemical fertilizer reduction; TF+70% CF, plot with Trichoderma fertilizer application and 30% chemical fertilizer reduction. DETAILED DESCRIPTION
[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0032] Preparation of potato glucose agar medium: weigh 15g potato powder, add 1000mL water, then add 20g glucose and 18g agar, sterilize at 121℃ for 20 minutes, divide into culture dishes, cool and store for later use; Preparation of potato glucose culture solution: weigh 15g potato powder, add 1000mL water, then add 20g glucose, sterilize at 121℃ for 20 minutes, cool and store for later use.
[0033] Example 1 (TF+70% CF)
[0034] The method for introducing Chinese wolfberry leaves into salinized soil in northern facilities comprises the following steps:
[0035] The vegetable cultivation area in Haiyang City, Yantai City, Shandong Province is large, and the vegetable greenhouses were built earlier. Many vegetable greenhouses over 20 years old have experienced secondary salinization, which has reduced the yield and planting income of conventional vegetables such as tomatoes. A vegetable greenhouse with secondary salinization was selected as the introduction test site. It is located in Miaotou Village, Haiyang City. The greenhouse is 23 years old and the surface soil salt content is 0.3%. Lycium barbarum was introduced from Guangxi Zhuang Autonomous Region and is a large-leaf wolfberry of the Lycium genus.
[0036] (1) The raw materials of the matrix are peat, perlite, peat and coconut bran, with an organic matter content of ≥45% and a neutral pH. In October 2021, cut the 15 cm long and 0.6 cm diameter shoots of the year from healthy wolfberry plants, and plant them in a seedling tray filled with the matrix. Keep the matrix moist. After about a month, capillary roots will grow at the bottom of the branches and set aside;
[0037] (2) In December 2021, dig out the wolfberry seedlings with the substrate, transplant them into the facility soil, and cultivate them in flat beds with a row spacing of 20 cm. Water them thoroughly immediately after transplanting. During the growing season, irrigate once a month in winter and twice a month in spring and autumn. In summer, the irrigation method is bed irrigation or drip irrigation, with a water volume of 0.12m each time. 3 / m 2 ;
[0038] (3) The first crop of leafy vegetables was harvested 60 days after planting, and then the first crop of leafy vegetables was harvested every 25 days;
[0039] (4) The facility should be ventilated every day except on rainy days, and once a week in spring and autumn. The ventilation time should be during the day, and the ventilation time should be 8-12 hours a day. The young branches and leaves should be harvested in time, and pesticides should be sprayed in the non-harvest period before the new branches and leaves sprout and after the leaves unfold. Spray 0.5 degrees Baume lime sulfur mixture to control powdery mildew and wolfberry gall mites, spray 500 times diluted 50% dimethoate to control psyllids and aphids, and spray 1000 times diluted 20% dicofol emulsifiable concentrate to control mites.
[0040] (5) The preparation method of Trichoderma fertilizer comprises the following steps:
[0041] 1) The apple pectin residue after pectin extraction is first subjected to natural composting to obtain organic compost;
[0042] 2) preparing a Trichoderma liquid from the Trichoderma acanthocereus strain by a shake flask method;
[0043] The Trichoderma asperellum strain is the Trichoderma asperellum disclosed in patent number ZL201710035544.5, which is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration with the deposit number CGMCC No.13187.
[0044] The method for preparing Trichoderma liquid from Trichoderma spp. strain by shaking flask method comprises the following steps: picking hyphae at the edge of potato glucose agar medium plate or scraping green spores, transferring hyphae or spores into potato glucose liquid medium, and culturing at 28° C. and 150 rpm for 4-5 days to form a large amount of white flocculent hyphae, thereby obtaining Trichoderma liquid.
[0045] 3) spraying the Trichoderma liquid evenly onto the spread organic compost obtained in step 1), wherein the inoculation amount of the Trichoderma liquid is controlled so that the volume mass ratio of the Trichoderma liquid to the organic compost is 10 L / 100 kg, and then stacking the materials into a trapezoidal pile shape, turning the pile every 3 days at room temperature, completing the Trichoderma fermentation in 7 days, spreading and airing the pile until the moisture content is 30%, and crushing the pile to obtain the Trichoderma fertilizer.
[0046] In January, Trichoderma fertilizer, urea, calcium magnesium phosphate fertilizer (active ingredient content of P2O5 ≥ 12%) and potassium sulfate were applied as basal fertilizer. Urea and potassium sulfate were applied every two months. Both basal fertilizer and topdressing fertilizer were turned into the soil. The application rate of Trichoderma fertilizer was 3.55 kg / m 2 The application amount of calcium magnesium phosphate fertilizer is 735.84g / m 2 The base application rate and topdressing rate of urea were 36.5 g / m 2 The base application rate and topdressing rate of potassium sulfate were 32.4 g / m 2 The annual application rates of total nitrogen, total phosphorus and total potassium are 240kg / ha, 160kg / ha and 240kg / ha respectively.
[0047] The seed introduction test site of Example 1 was selected to implement Comparative Examples 1-3.
[0048] Comparative Example 1 (CK, no nitrogen fertilizer control plot)
[0049] Except step (5) which is different from Example 1, other steps and parameters are the same as Example 1.
[0050] (5) Apply calcium magnesium phosphate fertilizer (with an effective ingredient content of P2O5 ≥ 12%) and potassium sulfate as basal fertilizer in January, and apply potassium sulfate every two months. Both basal fertilizer and topdressing fertilizer should be turned into the soil. The application rate of calcium magnesium phosphate fertilizer is 800g / m 2 The base application rate and topdressing rate of potassium sulfate were 46.2 g / m 2 The annual application rates of total phosphorus and total potassium are 160kg / ha and 240kg / ha.
[0051] Comparative Example 2 (100% CF, application of full fertilizer plot)
[0052] Except step (5) which is different from Example 1, other steps and parameters are the same as Example 1.
[0053] In January, urea, calcium magnesium phosphate fertilizer (with an effective ingredient content of P2O5 ≥ 12%) and potassium sulfate were applied as basal fertilizers. Urea and potassium sulfate were applied every two months. Both basal fertilizer and topdressing fertilizer were turned into the soil. The application rate of calcium magnesium phosphate fertilizer was 800g / m 2 The base application rate and topdressing rate of urea were 52.2 g / m 2The base application rate and topdressing rate of potassium sulfate were 46.2 g / m 2 The annual application rates of total nitrogen, total phosphorus and total potassium are 240kg / ha, 160kg / ha and 240kg / ha respectively.
[0054] Comparative Example 3 (OF+70%CF, organic fertilizer application and 30% reduction of chemical fertilizer application)
[0055] Except step (5) which is different from Example 1, other steps and parameters are the same as Example 1.
[0056] In January, organic fertilizer, urea, calcium magnesium phosphate fertilizer (active ingredient content is P2O5 ≥ 12%) and potassium sulfate were applied as basal fertilizer, and urea and potassium sulfate were applied every 2 months. The basal fertilizer and topdressing were turned into the soil. The organic fertilizer was the sterilized material of the Trichoderma fertilizer in Example 1, and the application amount was 3.55 kg / m 2 The application amount of calcium magnesium phosphate fertilizer is 735.84g / m 2 The base application rate and topdressing rate of urea were 36.5 g / m 2 The base application rate and topdressing rate of potassium sulfate were 32.4 g / m 2 The annual application rates of total nitrogen, total phosphorus and total potassium are 240kg / ha, 160kg / ha and 240kg / ha respectively.
[0057] Effect Experiment
[0058] Example 1 and Comparative Examples 1-3 were respectively arranged with 4 repeated plots, a total of 16 plots, arranged in random blocks, with a plot length of 1.5 m and a width of 1.4 m ( Figure 1 ). Compared with the control plot, the photosynthetic rate of wolfberry in the plots with full application of chemical fertilizers, reduced application of chemical fertilizers, and application of organic fertilizers and Trichoderma fertilizers during the growing season increased significantly, and the increase in the plot with Trichoderma fertilizers was the largest ( Figure 2 In July, the photosynthetic rate of wolfberry in the plot with Trichoderma fertilizer was not significantly different from that in the plot with organic fertilizer. However, in other months, the photosynthetic rate of wolfberry in the plot with Trichoderma fertilizer was significantly higher than that in the plot with full application of chemical fertilizer and organic fertilizer, indicating that the application of Trichoderma fertilizer is more conducive to enhancing the photosynthesis of wolfberry ( Figure 2 ). Compared with the control plot, the fresh weight of wolfberry leaves in the plots with full application of chemical fertilizers, reduced application of chemical fertilizers, and application of organic fertilizers and Trichoderma fertilizers increased significantly, and the increase in the plot with Trichoderma fertilizers reached 43.51%, which was significantly higher than the fresh weight of wolfberry leaves in the plots with full application of chemical fertilizers and organic fertilizers, indicating that the application of Trichoderma fertilizers can increase the yield of wolfberry leaf vegetables on the basis of reducing the application of chemical fertilizers ( Figure 3During the growing season, the application of full fertilizer, organic fertilizer and Trichoderma fertilizer significantly increased the content of soil mineral nitrogen. The content of soil mineral nitrogen in the Trichoderma fertilizer application area was significantly higher than that in the full fertilizer application area, and there was no significant difference with the organic fertilizer application area ( Figure 4 ).
[0059] In summary, compared with the application of full fertilizer, reducing chemical fertilizers and applying Trichoderma fertilizer significantly increased the supply of soil mineral nitrogen, which helped wolfberry nitrogen absorption, thereby increasing the photosynthetic rate and increasing leafy vegetable yields. The yield-increasing effect was better than that of applying organic fertilizers.
[0060] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A method for introducing Chinese wolfberry into salinized soil in northern facilities, characterized in that: The following steps are involved: (1) In autumn, cut the current year's shoots with a length of 10-15 cm and a diameter of 0.5-0.8 cm from healthy wolfberry plants, and plant them in a seedling tray filled with a substrate, or directly plant them in local soil. Keep the substrate or soil moist until capillary roots grow at the bottom of the shoots one month later, and set aside; (2) In November and December, dig out the wolfberry seedlings with the substrate or soil and transplant them into the facility soil. The salt content of the facility soil is less than 0.4%. Cultivate them on flat beds with a row spacing of 20 cm. Water them thoroughly immediately after transplanting. During the growing season, irrigate once a month in winter, 1-2 times a month in spring and autumn, and 3-4 times a month in summer. The irrigation method is bed irrigation or drip irrigation, and the irrigation volume is 0.10-0.12m 3 / m 2 ; (3) The first crop of leafy vegetables was harvested 60 days after planting, and then the next crop of leafy vegetables was harvested every 25-30 days; (4) Ensure ventilation in the facility, harvest tender branches and leaves in a timely manner, and spray new branches and leaves with pesticides during the non-harvest period; (5) In January, Trichoderma fertilizer was applied together with urea, calcium magnesium phosphate fertilizer and potassium sulfate as basal fertilizer. Urea and potassium sulfate were applied every two months. The basal fertilizer was turned into the soil, and the topdressing fertilizer was turned into the soil or applied with drip irrigation using water-soluble fertilizer. The application rate of Trichoderma fertilizer was 3.55 kg / m 2 The application amount of calcium magnesium phosphate fertilizer is 735.84g / m 2 The base application rate and topdressing rate of urea were 36.5 g / m 2 The base application rate and topdressing rate of potassium sulfate were 32.4 g / m 2 The annual application rates of total nitrogen, total phosphorus and total potassium are 240kg / ha, 160kg / ha and 240kg / ha respectively.
2. The method for introducing Chinese wolfberry into salinized soil in northern facilities according to claim 1, characterized in that: In step (5), the preparation method of Trichoderma fertilizer comprises the following steps: 1) The apple pectin residue after pectin extraction is first subjected to natural composting for 20-24 days to obtain organic compost; 2) preparing a Trichoderma liquid from the Trichoderma acanthocereus strain by a shake flask method; 3) spraying the Trichoderma liquid evenly onto the spread organic compost obtained in step 1), wherein the inoculation amount of the Trichoderma liquid is controlled so that the volume mass ratio of the Trichoderma liquid to the organic compost is (5-20) L / 100 kg, and then stacking the materials into a trapezoidal pile shape, turning the pile every 3 days at room temperature, completing the Trichoderma fermentation in 7 to 14 days, spreading and airing the pile until the moisture content is 30%, and crushing the pile to obtain the Trichoderma fertilizer.
3. The method for introducing Chinese wolfberry into salinized soil in northern facilities according to claim 2, characterized in that: In step 2), the method of preparing a Trichoderma liquid from the Trichoderma spp. strain by a shake flask method comprises the following steps: picking hyphae from the edge of a potato dextrose agar medium plate or scraping green spores, transferring the hyphae or spores into a potato dextrose liquid medium, and culturing at 28° C. and a rotation speed of 150 rpm for 4-5 days to form a large amount of white flocculent hyphae to obtain a Trichoderma liquid.
4. The method for introducing Chinese wolfberry into salinized soil in northern facilities according to claim 3, characterized in that: Preparation of potato glucose agar medium: weigh 15g potato powder, add 1000mL water, then add 20g glucose and 18g agar, sterilize at 121℃ for 20 minutes, divide into culture dishes, cool and store for later use; Preparation of potato glucose culture solution: weigh 15g potato powder, add 1000mL water, then add 20g glucose, sterilize at 121℃ for 20 minutes, cool and store for later use.
5. The method for introducing Chinese wolfberry into salinized soil in northern facilities according to claim 1, characterized in that: In step (1), the raw materials of the substrate are peat, perlite, peat and coconut husk, the organic matter content is ≥45%, and the pH is neutral.
6. The method for introducing Chinese wolfberry into salinized soil in northern facilities according to claim 1, characterized in that: The specific steps of step (4) are as follows: the facility is ventilated every day except on rainy days, once a week in spring and autumn, the ventilation time is during the day, the ventilation time is 8-12 hours a day, the tender branches and leaves are harvested in time, and pesticides are sprayed in the non-harvest period before the new branches and leaves sprout and after the leaves unfold, 0.5 degrees Baume lime sulfur mixture is sprayed to control powdery mildew and wolfberry gall mites, 500 times diluted 50% dimethoate is sprayed to control psyllids and aphids, and 1000 times diluted 20% dicofol emulsifiable concentrate is sprayed to control mites.
Citation Information
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