Construction process for treating endangered haloxylon ammodendron by using microbial technology

Abstract

The invention discloses a construction process for treating endangered haloxylon ammodendron trees by using a microbial technology. The construction process aims at solving the problem that withered haloxylon ammodendron trees cannot be rejuvenated and gradually die under the condition that a large amount of water cannot be watered in an extremely arid area. Comprising the following steps: step 1, preparing haloxylon ammodendron microbial bacterial liquid; step 2, preparing microbial minerals; step 4, backfilling microbial minerals; step 5, irrigating haloxylon ammodendron with microbial bacterial liquid; 6, backfilling the treatment pits with soil; the haloxylon ammodendron is irrigated for the first time for 5-7 days, the haloxylon ammodendron is irrigated for the second time, the haloxylon ammodendron microorganism bacterium liquid prepared according to the specification and sterile water are mixed according to the proportion of 1: 500, the mixture is put into a watering cart for water irrigation and then irrigated on site, and each haloxylon ammodendron is irrigated with 20 kg of bacterium liquid. The process realizes water-saving rejuvenation of haloxylon ammodendron trees in extreme drought and water-deficient areas by using a microbial technology, and is low in cost, high in efficiency, good in ecological benefit and water-saving.

Description

Technical Field

[0001] The invention relates to the technical field of Haloxylon ammodendron planting, in particular to a construction process for rescuing endangered Haloxylon ammodendron trees by using microbial technology. Background Art

[0002] The surface vegetation, primarily composed of Haloxylon ammodendron, Tamarix chinensis, Salix psammophila, Elaeagnus angustifolia, and Caragana korshinskii, maintains a fragile environmental balance. This is particularly true in southern Xinjiang, northern Qinghai, northern Gansu, northwestern Ningxia, and the vast Alxa League of Inner Mongolia. Haloxylon ammodendron serves as a key windbreak and sand fixation plant, playing a vital role in maintaining the ecological environment. However, in recent years, due to changing climatic and soil conditions, Haloxylon ammodendron trees over five years old have been withering and dying across these regions. In particular, in Alxa League of Inner Mongolia, nearly 4 million mu (approximately 1.5 acres) of Haloxylon ammodendron stands are withering or even dying. If these stunted or dying Haloxylon ammodendron trees are not promptly rejuvenated, not only will the ecological environment in these regions be severely damaged, but an ecological disaster will also directly threaten human survival.

[0003] The current commonly used method for rescuing and rejuvenating sick and withered Haloxylon ammodendron trees is water irrigation, which involves heavily watering sick and withered Haloxylon ammodendron trees in areas with fresh water sources, thereby rejuvenating a small number of Haloxylon ammodendron trees that withered due to drought and water shortage. This method has the following disadvantages: First, arid regions are generally short of water, especially those in Inner Mongolia, Xinjiang, Gansu, Qinghai, and Ningxia. It is impossible to use precious water to save the large number of sick and withered Haloxylon ammodendron trees. Only a very small area of ​​Haloxylon ammodendron trees can be replenished with water.

[0004] Second, research shows that single watering can only rejuvenate a small number of Haloxylon ammodendron trees that have withered purely due to drought and water shortage. Most Haloxylon ammodendron trees become sick and wither due to the combined effects of multiple factors such as climate, soil, and water supply. Therefore, single watering can only rejuvenate a very small number of withered Haloxylon ammodendron trees, and cannot fundamentally solve the problem of large-scale Haloxylon ammodendron tree sickness and withering.

[0005] Third, simply replenishing water will make the Haloxylon ammodendron trees dependent on water. Once the Haloxylon ammodendron trees, which have been rejuvenated through water replenishment, lose the additional water supply, they will wilt again. Simply replenishing water cannot fundamentally solve the problem of Haloxylon ammodendron wilting.

[0006] Rhizosphere microorganisms are a collective term for the bacteria, fungi, actinomycetes, and algae that live in the soil. They are an indispensable and vital component of the soil. Scientific evidence suggests that rhizosphere microorganisms are crucial factors in the interdependent relationship between trees and soil and in regulating tree growth. They are also a crucial means of improving soil structure and elemental variation, enhancing tree resistance and improving soil structure.

[0007] Tree roots are crucial for absorbing water and nutrients, acting as sensors for environmental signals and self-regulating growth. Mycorrhizae, a symbiotic system formed between fungi and tree roots, play a vital role in tree growth. Extant mycorrhizae can be divided into the following seven types: 1. Ectomycorrhizae; 2. Arbuscular mycorrhizae; 3. Ecto-endotrophic mycorrhizae; 4. Orchid mycorrhizae; 5. Arbutoid mycorrhizae; 6. Monotropoid mycorrhizae; and 7. Orchid mycorrhizae. Arbuscular mycorrhizae account for 72% of the biomass of known mycorrhizae. The functions of mycorrhizae are as follows: ① They can decompose difficult-to-decompose substances such as feldspar, apatite, peat, and lignin as nutrients; ② They can selectively absorb ions, accumulate high concentrations of heavy metals, and protect plants from heavy metal damage; ③ They can absorb phosphorus, nitrogen, and certain trace elements and transfer them to the host plant, especially low concentrations of soluble phosphorus, prevent the fixation of organic phosphorus, and enhance the drought resistance of plants; ④ Mycorrhizal fungi can secrete auxins such as gibberellins and indoleacetic acid during growth, which can promote plant growth; ⑤ Mycorrhizal fungi form fungal sheaths on the roots or occupy all cells of the roots to prevent invasion by other microorganisms; ⑥ Some mycorrhizal fungi can produce antibiotics and form a harmless microbial phase around them; ⑦ Mycorrhizae are also highly resistant to acid rain.

[0008] Plant hormones can regulate the growth and development of trees throughout their lifecycle. The growth and development of trees is controlled by the interaction of multiple hormones. With the discovery of multiple hormones and the synthesis of plant growth regulators, it has become possible to regulate and control the entire process of tree growth and development. The interaction of various hormones can regulate the entire process, including controlling tree growth, promoting regeneration, and inducing somatic dormancy, thereby improving trees' stress resistance. The interaction of various hormones, including strigolactones, auxins, cytokinins, ethylene, abscisic acid, and gibberellin, can regulate the entire process, including controlling tree growth, promoting regeneration, and inducing somatic dormancy, thereby improving trees' stress resistance.

[0009] Zeolite is a natural adsorbent that has attracted widespread attention due to its microporous structure and strong adsorption capacity. Zeolite also plays an important role in soil remediation. First, zeolite can use its microporous structure and surface chemical properties to adsorb pollutants in the soil, eliminating their threat to the ecological environment. Studies have shown that zeolite can effectively adsorb a variety of pollutants, including heavy metal ions, organic matter, and radioactive elements, and the amount of these pollutants adsorbed on zeolite is much higher than that of other adsorbents. Second, zeolite can also improve soil structure and soil quality. The microporous structure of zeolite can increase soil porosity, improve soil permeability and water retention, and promote the absorption of nutrients in the soil and plant growth. At the same time, due to its strong ion exchange capacity, zeolite can increase the cation exchange capacity of the soil and improve soil fertility.

[0010] Based on the above theoretical research, material properties and technical principles, and taking into account the core of the combination of microbial flora, minerals and plant hormones to promote the growth of the plant itself, stimulate the stress resistance of the plant body, improve the rhizosphere soil structure, and activate the nutrients that can be absorbed by plants in the soil, and according to the characteristics of plants and soil, an original construction process for using biotechnology to save endangered Haloxylon ammodendron trees has been developed. Summary of the Invention

[0011] In view of the above-mentioned technical deficiencies, the purpose of the present invention is to provide a construction process for rescuing endangered Haloxylon ammodendron trees using microbial technology, which solves the problem that withered and sick Haloxylon ammodendron trees cannot be rejuvenated and gradually die under the condition of inability to water in large quantities in extremely arid areas, and realizes the water-saving rejuvenation using microbial technology in extremely arid and water-scarce areas, with low cost, high efficiency, good ecological benefits and water saving.

[0012] In order to solve the above technical problems, the present invention adopts the following technical solutions: The present invention provides a construction process for rescuing endangered Haloxylon ammodendron trees using microbial technology, which is characterized by comprising the following steps: Step 1: Prepare Haloxylon ammodendron microbial solution: Mix the Haloxylon ammodendron microbial solution prepared according to regulations with sterile water at a ratio of 1:500 and pour into the water tank of the sprinkler truck; Step 2: Prepare microbial minerals: Pile 10kg of microbial minerals per Haloxylon ammodendron tree next to the tree for later use; Step 3: Dig a treatment pit: Use a trencher to dig a cylindrical pit with a diameter of 50 cm and a depth of 100 cm 30 cm away from the main root of the Haloxylon ammodendron tree; Step 4: Backfilling of microbial minerals: Mix the prepared microbial minerals with the excavated original soil in a ratio of 1:4, and then backfill them into the pit; Step 5: Watering with Haloxylon ammodendron microbial solution: Pour the Haloxylon ammodendron microbial solution prepared in the water tank of the sprinkler into the pit at a rate of 20 kg per Haloxylon ammodendron tree; Step 6: Backfill the treatment pit: After the Haloxylon ammodendron microbial solution has completely penetrated the treatment pit, take the excavated soil and backfill it to a depth of 50 cm. Step 7: Second irrigation of Haloxylon ammodendron microbial solution: 5 to 7 days after the first irrigation of Haloxylon ammodendron microbial solution, mix the Haloxylon ammodendron microbial solution prepared according to regulations and sterile water in a ratio of 1:500, put it into the water tank of the sprinkler truck and irrigate on site. Irrigate each Haloxylon ammodendron tree with 20 kg of solution.

[0013] Preferably, the method for preparing the Haloxylon ammodendron microbial liquid is as follows: the extracted and cultured Haloxylon ammodendron mycorrhizal fungi are separated, the separated Haloxylon ammodendron mycorrhizal fungi strains are placed in a culture medium and cultured for 15 to 20 days to form a Haloxylon ammodendron mycorrhizal fungi stock liquid, and then the Haloxylon ammodendron mycorrhizal fungi stock liquid is diluted with sterile water at a ratio of 1:2000 to form a Haloxylon ammodendron microbial liquid.

[0014] Preferably, the microbial mineral is prepared by mixing 80-mesh zeolite powder and Haloxylon ammodendron microbial powder, and the mixing ratio of the zeolite powder to the Haloxylon ammodendron microbial powder is 1000:5.

[0015] The beneficial effects of the present invention are: 1. The bacterial liquid used in this process is a small amount of liquid, and the water consumption is only one twentieth of the normal water replenishment amount of Haloxylon ammodendron. A small amount of water can solve the problem of rescuing and rejuvenating Haloxylon ammodendron trees over a large area.

[0016] 2. The microbial solution and microbial minerals used in this process act on both the Haloxylon ammodendron tree itself and the rhizosphere soil, allowing the microbial flora to inoculate into the tree's roots to form new root cap cells. Through the combined action of plant hormones and microorganisms, the Haloxylon ammodendron tree's resistance to drought, cold, salinity, and disease is enhanced. The microbial minerals improve the soil structure, adsorb and transform harmful substances, exchange charges to bury heavy metals, and activate beneficial elements for the tree's absorption. This fundamentally solves the problem of Haloxylon ammodendron tree disease and withering.

[0017] 3. Haloxylon ammodendron trees treated with this process do not require additional watering and will not wither again. Furthermore, after the trees have been treated and rejuvenated in the first year, they can be inoculated with plants such as Cistanche deserticola in the second year, laying a botanical foundation for the development of the sand industry in arid areas. While restoring vegetation and protecting the ecological environment, it also provides a solid foundation for the development of the sand industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 This is a construction flow chart of a construction process for rescuing endangered Haloxylon ammodendron trees using microbial technology, provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. Example 1

[0021] like Figure 1 As shown, a construction process for rescuing endangered Haloxylon ammodendron trees using microbial technology includes the following steps: Step 1: Preparation of Haloxylon ammodendron microbial liquid: Mix the Haloxylon ammodendron microbial liquid prepared according to regulations and sterile water at a ratio of 1:500 and pour the mixture into the water tank of a sprinkler truck; the preparation method of the Haloxylon ammodendron microbial liquid is as follows: isolate the extracted and cultured Haloxylon ammodendron mycorrhizal fungi, place the isolated Haloxylon ammodendron mycorrhizal fungi strains in a culture medium and culture them for 15 to 20 days to form a Haloxylon ammodendron mycorrhizal fungi stock liquid, and then dilute the Haloxylon ammodendron mycorrhizal fungi stock liquid with sterile water at a ratio of 1:2000 to form a Haloxylon ammodendron microbial liquid.

[0022] Step 2: Preparation of microbial minerals: pile 10 kg of microbial minerals per Haloxylon ammodendron tree next to the Haloxylon ammodendron tree for later use; the microbial minerals are prepared by mixing 80 mesh zeolite powder and Haloxylon ammodendron microbial powder, and the mixing ratio of zeolite powder to Haloxylon ammodendron microbial powder is 1000:5.

[0023] Step 3: Dig a treatment pit: Use a trencher to dig a cylindrical pit with a diameter of 50 cm and a depth of 100 cm 30 cm away from the main root of the Haloxylon ammodendron tree; Step 4: Backfilling of microbial minerals: Mix the prepared microbial minerals with the excavated original soil in a ratio of 1:4, and then backfill them into the pit; Step 5: Watering with Haloxylon ammodendron microbial solution: Pour the Haloxylon ammodendron microbial solution prepared in the water tank of the sprinkler into the pit at a rate of 20 kg per Haloxylon ammodendron tree; Step 6: Backfill the treatment pit: After the Haloxylon ammodendron microbial solution has completely penetrated the treatment pit, take the excavated soil and backfill it to a depth of 50 cm. Step 7: Second irrigation of Haloxylon ammodendron microbial solution: 5 to 7 days after the first irrigation of Haloxylon ammodendron microbial solution, mix the Haloxylon ammodendron microbial solution prepared according to regulations and sterile water in a ratio of 1:500, put it into the water tank of the sprinkler truck and irrigate on site. Irrigate each Haloxylon ammodendron tree with 20 kg of solution.

[0024] The microbial treatment technology for endangered Haloxylon ammodendron trees utilizes microbial technology, integrating beneficial bacteria, mycorrhizal fungi, plant hormones, and minerals into a comprehensive treatment system for the first time. This enables efficient and cost-effective treatment and rejuvenation of diseased and withered Haloxylon ammodendron trees, making large-scale restoration of withered Haloxylon ammodendron trees a reality. This represents a significant contribution to protecting the ecological environment and developing the sand industry. The microbial treatment technology for endangered Haloxylon ammodendron trees focuses on enhancing the tree's inherent stress resistance and improving the rhizosphere soil environment. Using a unique construction process, the microbial system organically integrates microorganisms, minerals, and the tree itself. This achieves rapid healing and rejuvenation, saves costs, conserves water resources, and protects the ecological environment.

[0025] 1. This process acts on plant cells and roots, effectively stimulating and significantly enhancing the plant's inherent resistance to stress, effectively reducing its dependence on water for growth and achieving the goal of rejuvenation with less water. This is of great significance in arid and water-scarce areas.

[0026] 2. This process acts on the rhizosphere soil, effectively decomposing harmful substances such as heavy metals in the soil, improving the soil structure, activating beneficial elements in the soil, and improving the soil's moisture retention capacity, forming a new rhizosphere soil suitable for the growth of Haloxylon ammodendron, providing a reliable guarantee for the normal growth of the plant; making it possible for Haloxylon ammodendron to grow normally in the long term.

[0027] 3. This process significantly conserves water resources. It uses only one twentieth of the water used by traditional methods, which is crucial in arid and water-scarce regions. This technology not only allows for the restoration of Haloxylon ammodendron trees in water-scarce areas, but also effectively restores and rejuvenates them in areas with insufficient or no water. This makes the restoration and rejuvenation of Haloxylon ammodendron trees in large areas of drought and water shortage a reality, which is crucial for protecting the fragile ecological environment in arid regions.

[0028] 4. This technology can be used to treat and rejuvenate sick and withered Haloxylon ammodendron trees, and parasitic plants such as Cistanche deserticola can be inoculated in the second year, which provides reliable conditions for the development of sand industry in arid areas and can greatly promote the regional economy and farmers' wealth.

[0029] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A construction process for curing endangered Haloxylon ammodendron trees using microbial technology, characterized in that: The steps include: Step 1: Preparation of Haloxylon ammodendron microbial liquid: Mix the Haloxylon ammodendron microbial liquid prepared according to regulations and sterile water at a ratio of 1:500 and pour into the water tank of a watering truck; the preparation method of the Haloxylon ammodendron microbial liquid is as follows: isolate the extracted and cultured Haloxylon ammodendron mycorrhizal fungi, place the isolated Haloxylon ammodendron mycorrhizal fungi strains in a culture medium and culture for 15 to 20 days to form a Haloxylon ammodendron mycorrhizal fungi stock liquid, and then dilute the Haloxylon ammodendron mycorrhizal fungi stock liquid with sterile water at a ratio of 1:2000 to form a Haloxylon ammodendron microbial liquid; Step 2: Preparation of microbial minerals: 10 kg of microbial minerals are piled up next to each Haloxylon ammodendron tree for later use; the microbial minerals are prepared by mixing 80-mesh zeolite powder and Haloxylon ammodendron microbial powder, and the mixing ratio of zeolite powder to Haloxylon ammodendron microbial powder is 1000:5; Step 3: Dig a treatment pit: Use a trencher to dig a cylindrical pit with a diameter of 50 cm and a depth of 100 cm 30 cm away from the main root of the Haloxylon ammodendron tree; Step 4: Backfilling of microbial minerals: Mix the prepared microbial minerals with the excavated original soil in a ratio of 1:4, and then backfill them into the pit; Step 5: Watering with Haloxylon ammodendron microbial solution: Pour the Haloxylon ammodendron microbial solution prepared in the water tank of the sprinkler into the pit at a rate of 20 kg per Haloxylon ammodendron tree; Step 6: Backfill the treatment pit with soil: After the Haloxylon ammodendron microbial solution irrigated into the treatment pit has completely penetrated, take the excavated original soil and backfill it to 50cm; Step 7: Secondary irrigation of Haloxylon ammodendron trees with microbial solution: 5 to 7 days after the first irrigation of Haloxylon ammodendron trees with microbial solution. Mix the Haloxylon ammodendron tree microbial solution prepared according to regulations and sterile water in a ratio of 1:500, load it into the water tank of a sprinkler truck and then water it on site. Each Haloxylon ammodendron tree should be watered with 20 kg of solution.

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