Wild-imitating cultivation method for armillaria mellea

Abstract

The invention relates to the technical field of armillaria mellea planting, in particular to a wild-imitating cultivation method for armillaria mellea. The invention provides a wild-imitating cultivation method for armillaria mellea. The wild-imitating cultivation method comprises the following steps: (1) soaking caragana korshinskii in a nutrient solution; (2) putting the soaked caragana korshinskii into the planting pit, scattering an armillaria mellea strain, performing cultivation after soil covering, and spraying a nutritional supplement once every 30-40 days; and (3) after fruiting, spraying a nutrition enhancer for 2-3 times every year. According to the wild-imitating cultivation method for the armillaria mellea, the caragana microphylla is pretreated, the utilization efficiency of the armillaria mellea on the caragana microphylla can be improved, nutritional supplements and nutrient supplements are additionally applied in the growth process and the fruiting process of the armillaria mellea, the fruiting time of the armillaria mellea can be remarkably shortened, and the yield of the armillaria mellea is improved. The duration of fruiting can be prolonged, the total yield of armillaria mellea can be improved, and the economic benefits of planting farmers are improved.

Description

Technical Field

[0001] The invention relates to the technical field of honey fungus cultivation, and in particular to a method for simulating wild cultivation of honey fungus. Background Art

[0002] Caragana is a plant of the genus Caragana in the Leguminosae family. It has a well-developed root system and strong resistance to adversity. It is one of the main windbreak and sand-fixing tree species in desert steppes. With the rapid expansion of Caragana planting area and the increase of forest age, untimely pruning has led to varying degrees of lignification and even serious death of early planted Caragana. Pruning can promote the germination of a large number of branches at the base of the plant stump, exert reproductive compensation ability, promote the growth and renewal of Caragana root system, and is an economical and effective measure to solve the problem of premature aging and death of Caragana.

[0003] In recent years, the research on the production of feed and biomass pellets from the pruning of Caragana korshinskii has not yet reached the level of large-scale commercial promotion. The reason is that the technology is immature and the economic benefits are low, which leads to low enthusiasm of farmers and herdsmen for pruning Caragana korshinskii. Therefore, how to reasonably develop and utilize the pruning branch resources of Caragana korshinskii is an urgent problem to be solved.

[0004] Honey fungus is a rare large fungus with high nutritional and medicinal value. It belongs to the phylum Basidiomycota, order Agaricales, family Physalacriaceae, and genus Armillaria (Fr.) Staude. Honey fungus, commonly known as hazel mushroom, is an edible fungus for many famous dishes such as chicken stewed with mushrooms. It has thick flesh, delicious taste and unique flavor, and is a delicacy on people's tables. In recent years, the market demand for honey fungus has been increasing year by year. However, the resources of wild honey fungus are limited and cannot meet people's demand for eating honey fungus all year round. Therefore, once the commercial cultivation of honey fungus is successful, the market prospect is huge.

[0005] In order to fully develop and sustainably utilize the resources of Caragana korshinskii, Caragana korshinskii can be used in the cultivation of Armillaria mellea, and a large number of Caragana korshinskii branches cut down can be transformed into Armillaria mellea with higher economic value, so that the abundant Caragana korshinskii resources can be efficiently utilized. For example, the document "Identification, Biological Characteristics and Domestication Cultivation of a Wild Armillaria by Liu Xueying" provides a technical solution for cultivating Armillaria mellea using Caragana korshinskii. However, this technology only involves simply scattering Armillaria mellea into Caragana korshinskii, and the utilization rate of Armillaria mellea for Caragana korshinskii is low, which not only leads to low yield of Armillaria mellea, but also a long fruiting cycle. It takes about 3 years until most of the Caragana korshinskii branches are converted into Armillaria mellea.

[0006] Therefore, how to provide a simulated wild cultivation method for Armillaria mellea to solve the technical problem in the prior art that Armillaria mellea has low utilization rate of Caragana korshinskii, resulting in a long fruiting cycle and low yield of Armillaria mellea is an issue that technical personnel in this field urgently need to solve. Summary of the invention

[0007] The purpose of the invention is to provide a wild-simulated cultivation method of Armillaria mellea.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The present invention provides a wild-simulated cultivation method of Armillaria mellea, comprising the following steps:

[0010] (1) Soaking Caragana korshinskii in nutrient solution;

[0011] The nutrient solution comprises the following components in parts by weight: 30-40 parts of tartary buckwheat extract, 20-30 parts of beet extract, 18-26 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 5-7 parts of sucrose, 3-5 parts of threonine, 2-3 parts of methionine, 1-2 parts of glutamic acid and 4-6 parts of chitosan;

[0012] (2) Place the soaked Caragana korshinskii into a planting pit, sprinkle with Armillaria mellea fungus, cover with soil and cultivate, and spray with nutritional supplements every 30 to 40 days;

[0013] The nutritional supplement comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 10-20 parts of beet extract, 12-16 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, and 160-200 parts of potassium dihydrogen phosphate;

[0014] (3) After the mushrooms start to grow, spray the nutrient enhancer 2 to 3 times each year;

[0015] The nutritional enhancer comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 12-16 parts of beet extract, 10-20 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, 80-120 parts of potassium dihydrogen phosphate, 20-30 parts of zinc sulfate and 20-30 parts of copper sulfate.

[0016] Preferably, the preparation method of the tartary buckwheat extract, beet extract and terminalia chebula extract comprises the following steps: mixing the raw material with the extractant, performing reflux extraction, repeating 2 to 3 times, combining the extracts and centrifuging to obtain the supernatant, recovering the extractant under reduced pressure and freeze-drying to obtain the extract.

[0017] Preferably, the ratio of the raw material to the extractant is 1 g: 6-10 mL; and the reflux extraction time is 60-80 min.

[0018] Preferably, the centrifugal speed is 6000-7000 rpm and the time is 12-16 min.

[0019] Preferably, when the raw material is buckwheat, the extractant is ethanol with a volume concentration of 72-78%; when the raw material is beet, the extractant is ethanol with a volume concentration of 62-68%; when the raw material is buckwheat, the extractant is ethyl acetate.

[0020] Preferably, the soaking time in step (1) is 2 to 4 hours; and the mass ratio of the Caragana korshinskii to the Armillaria mellea strain in step (2) is 100 kg: 4 to 8 kg.

[0021] Preferably, the time interval for spraying the nutrient enhancer in step (3) is 7 to 10 days.

[0022] Preferably, the preparation method of the nutrient solution in step (1) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, sucrose, threonine, methionine, glutamic acid and chitosan to obtain material 1; mixing the material 1 with water in a mass ratio of 1:15 to 20 to obtain a nutrient solution.

[0023] Preferably, the preparation method of the nutritional supplement in step (2) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea and potassium dihydrogen phosphate to obtain material 2; mixing material 2 with water in a mass ratio of 1:20 to 26 to obtain a nutritional supplement; the application amount of the nutritional enhancer is 180 to 240 mL / m 2 .

[0024] Preferably, the preparation method of the nutritional enhancer in step (3) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea, potassium dihydrogen phosphate, zinc sulfate and copper sulfate to obtain material 3; mixing material 3 with water in a mass ratio of 1:16-20 to obtain a nutritional enhancer; the application amount of the nutritional enhancer is 220-280 mL / m 2 .

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] In the prior art, most of them directly use Caragana korshinskii to cultivate Armillaria mellea, but it takes a long time for Armillaria mellea to colonize and propagate in Caragana korshinskii. In the present invention, Caragana korshinskii is soaked in a nutrient solution, and the nutrient solution contains the nutrient elements and growth factors required for the growth of Armillaria mellea, so that it can quickly colonize and propagate on Caragana korshinskii, and decompose and utilize the nutrient elements in Caragana korshinskii.

[0027] The applicant found in the previous experiments that the hyphae and mycelium of Armillaria mellea grow slowly, thin and weak on ordinary PDA culture medium, and the suitable culture medium for expanding the species is buckwheat culture medium, which is completely different from the suitable culture medium types of other Armillaria species reported in the prior art. It is speculated that the same species may not require the same nutrition for expanding the species due to different origins. The applicant further found through experiments that the phenolic substances in bitter buckwheat have a stimulating effect on the growth of Armillaria mellea. The present invention uses a reflux extraction method to prepare bitter buckwheat extract, which not only contains rich bitter buckwheat polyphenols, but also contains a variety of nutrients for Armillaria mellea to absorb and utilize. The present invention also adds beet extract to the nutrient solution, which has growth factors that can stimulate the growth of Armillaria mellea. The terminalia chebula extract added by the present invention has good antioxidant activity, and the addition of this component can help Armillaria mellea resist the stimulation of the external environment to improve the stress resistance of Armillaria mellea. Methyl jasmonate is a plant hormone. The addition of this component can stimulate the expression of the growth gene of Armillaria melleae, stimulate the ability of Armillaria melleae to produce bioactive enzymes such as amylase, protease, and laccase, so that Armillaria melleae can more quickly and effectively utilize the nutrients in Caragana korshinskii. Therefore, the four components of tartary buckwheat extract, beet extract, terminalia chebula extract and methyl jasmonate added by the present invention are added and used simultaneously, which has the functions of stimulating the growth of Armillaria melleae, providing nutrients, and improving the stress resistance of Armillaria melleae, so that Armillaria melleae can effectively utilize Caragana korshinskii.

[0028] The present invention also sprays a nutrient enhancer 2 to 3 times during the culture period before the honey fungus fruiting, which has the effect of nutritional supplementation and can also regulate the water content of the soil, which is helpful for the fruiting of honey fungus. The present invention also sprays a nutrient enhancer after the honey fungus fruiting, and the nutrient enhancer can enhance the fruiting stamina, promote mycelium growth, supplement nutrition, promote mushroom production and prolong the fruiting time. Therefore, the honey fungus simulated wild cultivation method provided by the present invention can improve the utilization efficiency of honey fungus for caragana by pre-treating caragana, and increase the nutrient supplement and nutrient enhancer during the growth process of honey fungus and fruiting, which can significantly shorten the fruiting time of honey fungus, prolong the duration of fruiting and increase the total yield of honey fungus, thereby improving the economic benefits of planting farmers. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0030] Figure 1This is a flow chart of the cultivation method of the prior art in Experimental Example 1; wherein a. Armillaria is inoculated into Caragana korshinskii branches; b and c. Mycelial cords grow out of Caragana korshinskii branches; d. Cultivated Armillaria fruiting bodies. DETAILED DESCRIPTION

[0031] The present invention provides a wild-simulated cultivation method of Armillaria mellea, comprising the following steps:

[0032] (1) Soaking Caragana korshinskii in nutrient solution;

[0033] (2) Place the soaked Caragana korshinskii into a planting pit, sprinkle with Armillaria mellea fungus, cover with soil and cultivate, and spray with nutritional supplements every 30 to 40 days;

[0034] (3) After the mushrooms start to grow, spray the nutrient enhancer 2 to 3 times each year;

[0035] In the present invention, in step (2), the nutritional supplement is preferably sprayed once every 32 to 38 days; more preferably every 34 to 36 days; and more preferably every 35 days.

[0036] In the present invention, in step (3), preferably the nutrient enhancer is sprayed three times.

[0037] The nutrient solution comprises the following components in parts by weight: 30-40 parts of tartary buckwheat extract, 20-30 parts of beet extract, 18-26 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 5-7 parts of sucrose, 3-5 parts of threonine, 2-3 parts of methionine, 1-2 parts of glutamic acid and 4-6 parts of chitosan; preferably 32-38 parts of tartary buckwheat extract, 22-28 parts of beet extract, 19-25 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 6 parts of sucrose, 4 parts of threonine, 2-3 parts of methionine, 1-2 parts of glutamic acid and 4-6 parts of chitosan. Acid: 3 parts, glutamic acid: 2 parts and chitosan: 5 parts; further preferably: 34-36 parts of tartary buckwheat extract, 24-26 parts of beet extract, 20-24 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 6 parts of sucrose, 4 parts of threonine, 3 parts of methionine, 2 parts of glutamic acid and 5 parts of chitosan; more preferably: 35 parts of tartary buckwheat extract, 25 parts of beet extract, 22 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 6 parts of sucrose, 4 parts of threonine, 3 parts of methionine, 2 parts of glutamic acid and 5 parts of chitosan.

[0038] The nutritional supplement comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 10-20 parts of beet extract, 12-16 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, and 160-200 parts of potassium dihydrogen phosphate; preferably, 22-28 parts of tartary buckwheat extract, 12-18 parts of beet extract, 13-15 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 220-280 parts of urea, and 170-190 parts of potassium dihydrogen phosphate; further preferably, 24-26 parts of tartary buckwheat extract, 14-16 parts of beet extract, 14 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 240-260 parts of urea, and 180 parts of potassium dihydrogen phosphate; more preferably, 25 parts of tartary buckwheat extract, 15 parts of beet extract, 14 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 250 parts of urea, and 180 parts of potassium dihydrogen phosphate.

[0039] The nutritional enhancer comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 12-16 parts of beet extract, 10-20 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, 80-120 parts of potassium dihydrogen phosphate, 20-30 parts of zinc sulfate, and 20-30 parts of copper sulfate; preferably 22-28 parts of tartary buckwheat extract, 13-15 parts of beet extract, 12-18 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 220-280 parts of urea, and 90-110 parts of potassium dihydrogen phosphate. , 22-28 parts of zinc sulfate, 22-28 parts of copper sulfate; further preferably 24-26 parts of tartary buckwheat extract, 14 parts of beet extract, 14-16 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 240-260 parts of urea, 100 parts of potassium dihydrogen phosphate, 24-26 parts of zinc sulfate, 24-26 parts of copper sulfate; more preferably 25 parts of tartary buckwheat extract, 14 parts of beet extract, 15 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 250 parts of urea, 100 parts of potassium dihydrogen phosphate, 25 parts of zinc sulfate, and 25 parts of copper sulfate.

[0040] In the present invention, the preparation method of the buckwheat extract, beet extract and terminalia chebula extract comprises the following steps: mixing the raw material with the extractant, refluxing extraction, repeating 2 to 3 times, combining the extracts and centrifuging to obtain the supernatant, recovering the extractant under reduced pressure and freeze-drying to obtain the extract; preferably repeating 3 times.

[0041] In the present invention, the ratio of the raw material to the extractant is 1 g: 6-10 mL; preferably 1 g: 7-9 mL; more preferably 1 g: 8 mL.

[0042] In the present invention, the reflux extraction time is 60 to 80 min, preferably 64 to 76 min, more preferably 68 to 72 min, and more preferably 70 min.

[0043] In the present invention, the centrifugal speed is 6000-7000 rpm, and the time is 12-16 min; preferably, the centrifugal speed is 6200-6800 rpm, and the time is 13-15 min; further preferably, the centrifugal speed is 6400-6600 rpm, and the time is 14 min; more preferably, the centrifugal speed is 6500 rpm, and the time is 14 min.

[0044] In the present invention, when the raw material is tartary buckwheat, the extractant is ethanol with a volume concentration of 72-78%, preferably 73-77%, more preferably 74-76%, and more preferably 75%.

[0045] In the present invention, when the raw material is beet, the extractant is ethanol with a volume concentration of 62-68%; preferably 63-67%; more preferably 64-66%; and more preferably 65%.

[0046] In the present invention, when the raw material is tartary buckwheat, the extractant is ethyl acetate.

[0047] In the present invention, the soaking time in step (1) is 2 to 4 hours, preferably 3 hours.

[0048] In the present invention, the mass ratio of the Caragana korshinskii to the Armillaria mellea strains in step (2) is 100kg:4-8kg; preferably 100kg:5-7kg; and more preferably 100kg:6kg.

[0049] In the present invention, the time interval for spraying the nutrient enhancer in step (3) is 7 to 10 days, preferably 7 to 9 days, and more preferably 7 days.

[0050] In the present invention, the preparation method of the nutrient solution in step (1) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, sucrose, threonine, methionine, glutamic acid and chitosan to obtain material 1; mixing the material 1 with water in a mass ratio of 1:15 to 20 to obtain a nutrient solution of 1:16 to 19; preferably 1:17 to 18; further preferably 1:18.

[0051] In the present invention, the preparation method of the nutritional supplement in step (2) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea and potassium dihydrogen phosphate to obtain material 2; mixing the material 2 with water in a mass ratio of 1:20 to 26 to obtain a nutritional supplement; preferably 1:21 to 25; further preferably 1:22 to 24; more preferably 1:23.

[0052] In the present invention, the dosage of the nutritional enhancer is 180-240 mL / m 2 ; preferably 190~230mL / m 2; More preferably, 200 to 220 mL / m 2 More preferably, 200 mL / m 2 .

[0053] In the present invention, the preparation method of the nutritional enhancer in step (3) is: mixing tartary buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea, potassium dihydrogen phosphate, zinc sulfate and copper sulfate to obtain material 3; mixing the material 3 with water in a mass ratio of 1:16 to 20 to obtain the nutritional enhancer; preferably 1:17 to 19; more preferably 1:18.

[0054] In the present invention, the dosage of the nutritional enhancer is 220-280 mL / m 2 ; preferably 230~270mL / m 2 ; More preferably 240 to 260 mL / m 2 More preferably, 250 mL / m 2 .

[0055] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0056] Example 1

[0057] The present invention provides a wild-simulated cultivation method of Armillaria mellea, which comprises the following steps:

[0058] (1) Preparation of buckwheat extract: Buckwheat was mixed with 72% ethanol in a ratio of 1 g:6 mL, and refluxed for 60 min. The extraction was repeated twice. The combined extracts were centrifuged at 6000 rpm for 12 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain the buckwheat extract.

[0059] (2) Preparation of beet extract: beet and 62% ethanol by volume were mixed in a ratio of 1 g:6 mL, and refluxed for 60 min. This was repeated twice. The combined extracts were centrifuged at 6000 rpm for 12 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain a beet extract.

[0060] (3) Preparation of Terminalia chebula extract: Terminalia chebula was mixed with ethyl acetate in a ratio of 1 g:6 mL, and refluxed for 60 min. This was repeated twice. The combined extracts were centrifuged at 6000 rpm for 12 min to obtain the supernatant. The ethyl acetate was recovered under reduced pressure at 42° C. and then freeze-dried to a water content of 4% to obtain the Terminalia chebula extract.

[0061] (4) Preparation of nutrient solution: 30 parts of tartary buckwheat extract, 20 parts of beet extract, 18 parts of terminalia chebula extract, 4 parts of methyl jasmonate, 5 parts of sucrose, 3 parts of threonine, 2 parts of methionine, 1 part of glutamic acid and 4 parts of chitosan are mixed to obtain material 1; the material 1 is mixed with water at a mass ratio of 1:15 to obtain a nutrient solution;

[0062] (5) Place caragana korshinskii (about 50 cm long) in a pot and add nutrient solution until the nutrient solution just covers the caragana korshinskii. Soak for 2 h.

[0063] (6) Preparation of nutritional supplement: 20 parts of tartary buckwheat extract, 10 parts of beet extract, 12 parts of terminalia chebula extract, 4 parts of methyl jasmonate, 200 parts of urea, and 160 parts of potassium dihydrogen phosphate were mixed to obtain material 2; the material 2 was mixed with water at a mass ratio of 1:20 to obtain a nutritional supplement;

[0064] (7) Place the soaked Caragana korshinskii in the planting pit, evenly place the honey fungus in the Caragana korshinskii branch pile, cover with soil about 3 cm, and cover the soil layer with 5 cm thick wheat straw. During the cultivation process, the soil moisture is always maintained at 55%. Spray the nutrient supplement once every 30 days at an application rate of 180 mL / m 2 ; The mass ratio of Caragana korshinskii to Armillaria mellea strains is 100kg:4kg;

[0065] (8) Preparation of a nutritional enhancer: 20 parts of tartary buckwheat extract, 12 parts of beet extract, 10 parts of terminalia chebula extract, 4 parts of methyl jasmonate, 200 parts of urea, 80 parts of potassium dihydrogen phosphate, 20 parts of zinc sulfate, and 20 parts of copper sulfate were mixed to obtain a material 3; the material 3 was mixed with water at a mass ratio of 1:16 to obtain a nutritional enhancer;

[0066] (9) After the mycelium and mycelium grew on the branches for 13 months, they began to produce mushrooms for 30 days. After the mushrooms began to produce, the nutrient enhancer was sprayed twice at a rate of 220 mL / m 2 The time interval between each spraying is 7 days.

[0067] Example 2

[0068] The present invention provides a wild-simulated cultivation method of Armillaria mellea, which comprises the following steps:

[0069] (1) Preparation of buckwheat extract: Buckwheat was mixed with 78% ethanol in a ratio of 1 g:10 mL, and refluxed for 80 min. This was repeated three times. The combined extracts were centrifuged at 7000 rpm for 16 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain the buckwheat extract.

[0070] (2) Preparation of beet extract: beet and 68% ethanol by volume were mixed in a ratio of 1 g:10 mL, and refluxed for 80 min. This was repeated three times. The combined extracts were centrifuged at 7000 rpm for 16 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain a beet extract.

[0071] (3) Preparation of Terminalia chebula extract: Terminalia chebula was mixed with ethyl acetate in a ratio of 1 g:10 mL, and refluxed for 80 min. This was repeated three times. The extracts were combined and centrifuged at 7000 rpm for 16 min to obtain the supernatant. The ethyl acetate was recovered under reduced pressure at 42° C. and then freeze-dried to a water content of 4% to obtain the Terminalia chebula extract.

[0072] (4) Preparation of nutrient solution: 40 parts of tartary buckwheat extract, 30 parts of beet extract, 26 parts of terminalia chebula extract, 6 parts of methyl jasmonate, 7 parts of sucrose, 5 parts of threonine, 3 parts of methionine, 2 parts of glutamic acid and 6 parts of chitosan are mixed to obtain material 1; the material 1 is mixed with water at a mass ratio of 1:20 to obtain a nutrient solution;

[0073] (5) Place caragana korshinskii (about 50 cm long) in a pot and add nutrient solution until the nutrient solution just covers the caragana korshinskii. Soak for 4 h.

[0074] (6) Preparation of nutritional supplement: 30 parts of tartary buckwheat extract, 20 parts of beet extract, 16 parts of terminalia chebula extract, 6 parts of methyl jasmonate, 300 parts of urea, and 200 parts of potassium dihydrogen phosphate were mixed to obtain material 2; the material 2 was mixed with water at a mass ratio of 1:26 to obtain a nutritional supplement;

[0075] (7) Place the soaked Caragana korshinskii in the planting pit, evenly place the honey fungus in the Caragana korshinskii branch pile, cover with soil about 3 cm, and cover the soil layer with 7 cm thick straw. During the cultivation process, the soil moisture is always maintained at 60%. Spray the nutrient supplement once every 40 days at an application rate of 240 mL / m 2 ; The mass ratio of Caragana korshinskii to Armillaria mellea strains is 100kg:8kg;

[0076] (8) Preparation of a nutritional enhancer: 30 parts of tartary buckwheat extract, 16 parts of beet extract, 20 parts of terminalia chebula extract, 6 parts of methyl jasmonate, 300 parts of urea, 120 parts of potassium dihydrogen phosphate, 30 parts of zinc sulfate, and 30 parts of copper sulfate were mixed to obtain a material 3; the material 3 was mixed with water at a mass ratio of 1:20 to obtain a nutritional enhancer;

[0077] (9) After the mycelium and mycelium grew on the branches for 12 months, they began to produce mushrooms for 40 days. After the mushrooms began to produce, the nutrient enhancer was sprayed three times at a rate of 280 mL / m 2 The time interval between each spraying is 10 days.

[0078] Example 3

[0079] The present invention provides a wild-simulated cultivation method of Armillaria mellea, which comprises the following steps:

[0080] (1) Preparation of buckwheat extract: Buckwheat was mixed with 75% ethanol in a ratio of 1 g:8 mL, and refluxed for 70 min. This was repeated three times. The combined extracts were centrifuged at 6500 rpm for 14 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain the buckwheat extract.

[0081] (2) Preparation of beet extract: beet and 65% ethanol by volume were mixed in a ratio of 1 g:8 mL, and refluxed for 70 min. This was repeated three times. The combined extracts were centrifuged at 6500 rpm for 14 min to obtain the supernatant. The ethanol was recovered under reduced pressure at 42°C and then freeze-dried to a water content of 4% to obtain the beet extract.

[0082] (3) Preparation of Terminalia chebula extract: Terminalia chebula was mixed with ethyl acetate in a ratio of 1 g:8 mL, and refluxed for 70 min. This was repeated three times. The combined extracts were centrifuged at 6500 rpm for 14 min to obtain the supernatant. The ethyl acetate was recovered under reduced pressure at 42° C. and then freeze-dried to a water content of 4% to obtain the Terminalia chebula extract.

[0083] (4) Preparation of nutrient solution: 35 parts of tartary buckwheat extract, 25 parts of beet extract, 22 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 6 parts of sucrose, 4 parts of threonine, 3 parts of methionine, 2 parts of glutamic acid and 5 parts of chitosan are mixed to obtain material 1; the material 1 is mixed with water at a mass ratio of 1:18 to obtain a nutrient solution;

[0084] (5) Place caragana korshinskii (about 50 cm long) in a pot and add nutrient solution until the nutrient solution just covers the caragana korshinskii. Soak for 3 h.

[0085] (6) Preparation of nutritional supplement: 25 parts of tartary buckwheat extract, 15 parts of beet extract, 14 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 250 parts of urea, and 180 parts of potassium dihydrogen phosphate were mixed to obtain material 2; the material 2 was mixed with water at a mass ratio of 1:23 to obtain a nutritional supplement;

[0086] (7) Place the soaked Caragana korshinskii in the planting pit, evenly place the honey fungus in the Caragana korshinskii branch pile, cover with soil about 3 cm, and cover the soil layer with 6 cm thick wheat straw. During the cultivation process, the soil moisture is always maintained at 58%. Spray the nutrient supplement once every 35 days at an application rate of 200 mL / m 2 ; The mass ratio of Caragana korshinskii to Armillaria mellea strains is 100kg:6kg;

[0087] (8) Preparation of a nutritional enhancer: 25 parts of tartary buckwheat extract, 14 parts of beet extract, 15 parts of terminalia chebula extract, 5 parts of methyl jasmonate, 250 parts of urea, 100 parts of potassium dihydrogen phosphate, 25 parts of zinc sulfate, and 25 parts of copper sulfate were mixed to obtain material 3; the material 3 was mixed with water at a mass ratio of 1:18 to obtain a nutritional enhancer;

[0088] (9) After the mycelium and mycelium grew on the branches for 11 months, they began to produce mushrooms for 35 days. After the mushrooms began to produce, the nutrient enhancer was sprayed three times at a rate of 250 mL / m 2 The time interval between each spraying is 7 days.

[0089] Experimental Example 1

[0090] An experiment on the simulated wild cultivation of Armillaria mellea was conducted in a farmer's vegetable garden in the suburbs of Hohhot, Inner Mongolia. The varieties of Armillaria mellea were screened by the inventors, and the source document was "Identification, Biological Characteristics and Domestication Cultivation of a Wild Armillaria Strain by Liu Xueying". Three plots were treated in each experimental group.

[0091] The cultivation method provided in Example 3 was used as the experimental group.

[0092] Control group 1: other methods are the same as those in Example 3, except that the tartary buckwheat extract, beet extract, terminalia chebula extract and methyl jasmonate in the nutrient solution, nutrient supplement and nutrient enhancer are replaced by an equal amount of distilled water.

[0093] Control group 2: other methods are the same as those of Example 3, except that the tartary buckwheat extract and beet extract in the nutrient solution, nutrient supplement and nutrient enhancer are replaced with an equal amount of distilled water.

[0094] Control group 3: other methods are the same as those of Example 3, except that the tartary buckwheat extract in the nutrient solution, the nutritional supplement and the nutritional enhancer is replaced with an equal amount of distilled water.

[0095] Control group 4: other methods were the same as those in Example 3, except that the tartary buckwheat extract, beet extract and terminalia chebula extract in the nutrient solution were replaced with an equal amount of distilled water.

[0096] Control group 5: other methods were the same as those in Example 3, except that the Caragana korshinskii was not soaked in the nutrient solution.

[0097] Control group 6: other methods are the same as those of Example 3, except that the nutritional supplement is replaced by an equal amount of distilled water.

[0098] The existing technical group refers to the cultivation method in the document "Identification, biological characteristics and domestication and cultivation of a wild honey fungus by Liu Xueying".

[0099] Except for the different cultivation methods, all other growth management methods were the same for each experimental group. After the fruiting began, the time from planting to the beginning of fruiting and the duration of fruiting of Armillaria mellea were investigated in different experimental groups, and the fresh mushroom yield was counted. The results were averaged, as shown in Table 1.

[0100] Table 1 Questionnaire on mushroom production

[0101] Group Start of mushroom production Duration <![CDATA[Output kg / m 2 <!-- 7 -->]]> Experimental Group 11.2 months 38.6d 4.26 Control group 1 16.2 months 32.9d 2.84 Control group 2 15.5 months 33.3d 2.98 Control group 3 15.1 months 33.8d 3.11 Control group 4 15.8 months 34.0d 3.92 Control group 5 16.4 months 34.6d 3.67 Control group 6 14.7 months 35.5d 3.85 Existing Technology Group 18.3 months 31.5d 2.45

[0102] It is not difficult to see from the contents recorded in Table 1 that the cultivation method provided by the present invention can significantly shorten the fruiting time of hazel mushrooms, increase the duration of fruiting, and thus increase the total yield of hazel mushrooms, indicating that the cultivation method is significantly superior to the existing technology group.

[0103] From the data of the control group, it can be seen that the use of the nutrient solution, nutritional supplement and nutritional enhancer of the present invention has a significant promoting effect on the growth of hazel mushrooms, and whether the bitter buckwheat extract, beet extract, terminalia chebula extract and methyl jasmonate are added has the most significant effect on the use effect of the preparation. There is a synergistic effect between the bitter buckwheat extract, beet extract and terminalia chebula extract. If one of the components is discarded, the use effect of the preparation will also be significantly affected.

[0104] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for simulating wild cultivation of Armillaria mellea, characterized in that: The steps include: (1) Soaking Caragana korshinskii in nutrient solution; The nutrient solution comprises the following components in parts by weight: 30-40 parts of tartary buckwheat extract, 20-30 parts of beet extract, 18-26 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 5-7 parts of sucrose, 3-5 parts of threonine, 2-3 parts of methionine, 1-2 parts of glutamic acid and 4-6 parts of chitosan; (2) Place the soaked Caragana korshinskii into a planting pit, sprinkle with Armillaria mellea fungus, cover with soil and cultivate, and spray with nutritional supplements every 30 to 40 days; The nutritional supplement comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 10-20 parts of beet extract, 12-16 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, and 160-200 parts of potassium dihydrogen phosphate; (3) After the mushrooms start to grow, spray the nutrient enhancer 2 to 3 times each year; The nutritional enhancer comprises the following components in parts by weight: 20-30 parts of tartary buckwheat extract, 12-16 parts of beet extract, 10-20 parts of terminalia chebula extract, 4-6 parts of methyl jasmonate, 200-300 parts of urea, 80-120 parts of potassium dihydrogen phosphate, 20-30 parts of zinc sulfate and 20-30 parts of copper sulfate.

2. The cultivation method according to claim 1, characterized in that The preparation method of the tartary buckwheat extract, beet extract and terminalia chebula extract comprises the following steps: mixing the raw material with the extractant, performing reflux extraction, repeating the steps 2-3 times, combining the extracts and centrifuging to obtain the supernatant, recovering the extractant under reduced pressure and freeze-drying to obtain the extract.

3. The cultivation method according to claim 2, characterized in that: The ratio of the raw material to the extractant is 1 g: 6-10 mL; the time of the reflux extraction is 60-80 min.

4. The cultivation method according to claim 2, characterized in that: The centrifugal speed is 6000-7000 rpm, and the time is 12-16 minutes.

5. The cultivation method according to claim 2, characterized in that: When the raw material is bitter buckwheat, the extractant is ethanol with a volume concentration of 72-78%; when the raw material is beet, the extractant is ethanol with a volume concentration of 62-68%; when the raw material is bitter buckwheat, the extractant is ethyl acetate.

6. The cultivation method according to claim 1, characterized in that: The soaking time in step (1) is 2 to 4 hours; the mass ratio of the Caragana korshinskii to the Armillaria mellea strain in step (2) is 100kg:4 to 8kg.

7. The cultivation method according to claim 1, characterized in that: The time interval for spraying the nutrient enhancer in step (3) is 7 to 10 days.

8. The cultivation method according to claim 1, characterized in that: The preparation method of the nutrient solution in step (1) is as follows: bitter buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, sucrose, threonine, methionine, glutamic acid and chitosan are mixed to obtain material 1; the material 1 is mixed with water in a mass ratio of 1:15 to 20 to obtain a nutrient solution.

9. The cultivation method according to claim 1, characterized in that: The preparation method of the nutritional supplement in step (2) is as follows: bitter buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea and potassium dihydrogen phosphate are mixed to obtain material 2; the material 2 is mixed with water in a mass ratio of 1:20 to 26 to obtain a nutritional supplement; the application amount of the nutritional enhancer is 180 to 240 mL / m 2 .

10. The cultivation method according to claim 1, characterized in that: The preparation method of the nutritional enhancer in step (3) is as follows: bitter buckwheat extract, beet extract, terminalia chebula extract, methyl jasmonate, urea, potassium dihydrogen phosphate, zinc sulfate and copper sulfate are mixed to obtain material 3; the material 3 is mixed with water in a mass ratio of 1:16-20 to obtain the nutritional enhancer; the application amount of the nutritional enhancer is 220-280 mL / m 2 .

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