Method for cultivating edible fungus leather based on pressure regulation and control

Abstract

The invention discloses a method for cultivating edible fungus leather based on pressure regulation, which is characterized in that directional growth of mycelia is regulated by applying vertical pressure in stages, a compact bionic fiber structure is formed, and a high-performance environment-friendly material is obtained by combining physical post-treatment. The method breaks through the bottleneck that a traditional mycelium material is low in strength and depends on chemical treatment and is suitable for the fields of clothes, home furnishing and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of biomaterial manufacturing, and in particular to a solid culture method for regulating the growth direction and density of mycelium by applying controllable pressure, which is used for preparing environmentally friendly materials with leather-like physical properties and their applications. Background Art

[0002] Mycelium materials have the advantages of a wide range of resources and low production costs, and are gradually gaining attention. As a new type of biodegradable material without animal components, mycelium materials are the second choice for leather in the future. However, traditional mycelium cultivation mostly relies on natural growth, and the mycelium is loosely distributed. Later compression or chemical treatment is required to increase the density of the material. The process is complicated and may introduce pollution. Existing pressure treatment is mostly implemented after the mycelium matures (such as hot pressing molding), and it is impossible to directionally regulate the mycelium network structure during the growth stage.

[0003] The process of deep fermentation combined with solid culture is complex and costly (for example, liquid fermentation and solid culture need to be operated alternately in 2023107066583). In the prior art, the performance is improved by adding adhesives or post-treatment coatings (2020800376776, 2024107202667), which also adds multiple steps and is not conducive to the advancement of industrialization; the addition of chemical agents will also increase the risk of pollution. Summary of the invention

[0004] In response to the above technical problems, the present invention provides a method for regulating the directional growth of mycelium through dynamic pressure, directly forming a dense, high-strength leather-like structure, reducing chemical post-treatment, and realizing environmentally friendly and scalable mycelium leather preparation.

[0005] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions: 1. Activation and propagation of bacterial strains Select edible fungi with fast mycelial growth and few branches (such as Pleurotus ostreatus, Ganoderma lucidum, Pleurotus eryngii, Flammulina velutipes, Hericium erinaceus, etc.), and purify them for three generations on PDA plates; 2. Preparation of solid culture medium Matrix composition (weight ratio): cottonseed hull 50-70%, corn cob 10-20%, bran 15-30%, bagasse 5-10%, calcium carbonate 1-5%; the moisture content is adjusted to 65%, and sterilized at 121°C for 120-150 minutes.

[0006] 3. Cultivation Spread the sterilized culture medium in the culture tank with a thickness of 8-10 cm; control the environmental parameters after inoculating the strain in step 1: temperature 25-28℃, humidity 85-90%, CO2 concentration maintained at 8000-12000ppm (can be achieved through medium gas control technology). Pre-culture for 3-5 days under dark conditions until the mycelium covers the surface of the culture medium.

[0007] 4. Pressure loading and dynamic regulation Pressure application device: Use a multi-layer adjustable pressure culture device (refer to 201821879837.8), including a pressure sensor, a controller and a temperature sensor, etc. Maintain a temperature of 24-28°C, a humidity of 70-80%, and a CO2 concentration of ≤20000 ppm.

[0008] Pressure parameters: (1) Vertical pressure: Apply static pressure of 0.05-0.3 MPa in stages. The initial low pressure (0.05-0.1 MPa) guides the mycelium to extend along the horizontal plane; the later high pressure (0.2-0.3 MPa) promotes the densification of mycelium.

[0009] (2) Dynamic regulation: The pressure is adjusted in real time according to the mycelium growth rate, and the pressure fluctuation amplitude is ≤10% to avoid mycelium breakage.

[0010] 5. Post-processing and molding Termination of cultivation: After the mycelium forms a continuous film layer, stop pressurizing and dry at 40-60℃ until the moisture content is ≤10%.

[0011] Surface treatment: Physical calendering or natural coatings (such as natural resins and beeswax) are used to enhance surface smoothness and wear resistance. DETAILED DESCRIPTION

[0012] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work fall within the scope of protection of the present invention.

[0013] A method for cultivating edible fungus leather by extrusion is as follows: 1. Activation of fungus: Take out the frozen or dried fungus, inoculate it onto the surface of PDA culture medium through aseptic technique, and place it under suitable temperature conditions to promote the growth of fungus.

[0014] 2. Preparation of bacterial strains: Subculture the activated bacterial strains, that is, transfer part of the culture to a new plate culture medium, and use it as the mother culture after the plate is full.

[0015] 3. Preparation and inoculation of culture medium: 70% sawdust, 25% wheat bran, 5% gypsum, 60% water content. After mixing the culture medium materials, adjust the pH value to 7. After sterilization for 150 minutes, cool it and then inoculate it. Pressure loading is carried out 3 days after the initial culture.

[0016] 4. Pressure loading: Days 4-7: 0.08 MPa static pressure to induce horizontal growth; Day 8-14: Stepwise increase of pressure to 0.25 MPa, with a daily increase of 0.05 MPa.

[0017] 5. Harvesting: The thickness of the mycelium layer obtained was 1.2 mm, and its strength was close after simple post-treatment.

Claims

1. A method for cultivating edible fungus leather based on pressure regulation, characterized in that The following steps are involved: Mycelium solid culture, staged static / dynamic pressure loading, directional growth regulation and post-processing molding.

2. The method according to claim 1, characterized in that The pressure loading is in the vertical direction, with an initial pressure of 0.05-0.1 MPa, which increases to 0.2-0.3 MPa in the later stage.

3. The method according to claim 1, characterized in that The pulse pressure mode is adopted, with a frequency of 1-5 times / hour and an amplitude of 0.02-0.1 MPa.

4. The method according to claim 1, wherein the post-treatment comprises physical calendering or natural coating impregnation, and the coating component is at least one of natural resin and beeswax.