Grifola frondosa log mushroom stick, manufacturing method and application thereof

Abstract

The invention relates to edible fungus cultivation, in particular to a grifola frondosa log fungus stick and a manufacturing method and application thereof. The grifola frondosa log fungus stick comprises a culture vessel and a culture material; the culture vessel is a hollow Chinese chestnut log, and the culture material is placed in the hollow position of the Chinese chestnut log. According to the method, the mushroom sticks and the growth mode of the grifola frondosa are optimized, the sustainable and good-stability grifola frondosa cultivation method is provided, the growth mode of wild grifola frondosa can be simulated, the mushroom sticks can achieve continuous fruiting for many years, the nutritive value of the grifola frondosa is increased, the yield of the grifola frondosa is increased, the production cost of enterprises is reduced, and economic benefits are increased.

Description

Technical Field

[0001] The invention relates to edible fungus cultivation, in particular to a Grifola frondosa log mushroom stick, a preparation method and application thereof. Background Art

[0002] Grifola frondosa, also known as chestnut mushroom, bay leaf polypore, lotus mushroom, etc., is a precious edible and medicinal mushroom. Its fruiting body resembles a blooming lotus. The fresh fruiting body has a strong fragrance and is rich in amino acids, polysaccharides and bioactive substances. It has the effect of enhancing human immunity and preventing various diseases. In recent years, as a high-end health food, it has become popular in the market and is one of the main edible mushroom varieties vigorously promoted by the Food and Agriculture Organization of the United Nations. At present, its artificial cultivation matrix is ​​mainly sawdust and cottonseed shells. The cultivation of Grifola frondosa is to inoculate the bacterial liquid onto the mushroom stick, and then the mushroom stick is directly cultivated to produce mushrooms, and the utilization rate of the mushroom stick is relatively low. Wild Grifola frondosa occurs around the roots of chestnut trees in summer and autumn, as well as around the trunks and stakes of broad-leaved trees such as oak and Castanopsis, causing wood decay, and has extremely high nutritional value. It is now necessary to study a Grifola frondosa mushroom stick that can simulate the growth pattern of wild Grifola frondosa, and the mushroom stick can achieve continuous mushroom production for many years, improve the nutritional value of Grifola frondosa, increase the yield of Grifola frondosa, reduce the production cost of enterprises, and increase economic benefits. Summary of the invention

[0003] In order to solve the problems in the prior art of low utilization rate of Grifola frondosa mushroom sticks and high Grifola frondosa cultivation costs during Grifola frondosa cultivation, a Grifola frondosa log mushroom stick, a preparation method and application thereof are proposed.

[0004] In order to achieve the above purpose, the technical solutions adopted are as follows: A Grifola frondosa log mushroom stick comprises a culture vessel and culture medium; the culture vessel is a hollow chestnut log, and the culture medium is placed in the hollow position of the chestnut log.

[0005] An application of a Grifola frondosa log mushroom stick is to cultivate and produce mushrooms using the Grifola frondosa log mushroom stick in any one of a simulated wild soil covering mode, a layer rack mode, and a layer rack soil covering coupling mode.

[0006] Preferably, the simulated wild soil covering mode is specifically: Dig grooves on the ground under the chestnut forest, place the Grifola frondosa logs evenly in the grooves, and cover the surface with 2-5 cm of raw soil after arranging them.

[0007] Preferably, the shelf mode is specifically: In the greenhouse, the raw wood mushroom sticks are evenly placed on the mushroom racks. The temperature and humidity in the greenhouse are controlled and ventilated. Mushrooms will grow at the mushroom holes after 50-75 days.

[0008] Preferably, the layer frame soil covering coupling type is specifically: The fruiting port of the Grifola frondosa log fruiting stick after fruiting in the shelf mode is drilled again with a clean drill bit to the internal substitute material and the debris is cleaned, and the liquid strain is inoculated at the drilled hole for the second time, and the hole is sealed with absorbent cotton after the strain is inoculated; The Grifola frondosa logs after the second inoculation are used for excretion and cultivation in a simulated wild soil covering mode.

[0009] A method for making a Grifola frondosa log mushroom stick comprises the following steps: Pretreatment of chestnut logs S1: Select appropriate chestnut logs; S2: removing the central wood core of the chestnut log; S3: drilling a hole on the outer wall of the chestnut log to connect with the hollow part of the chestnut log; S4: Spraying the surface of the chestnut log with the treatment liquid; S5: flash sterilizing the treated chestnut logs for later use; Pretreatment of culture medium S1: Bag the culture medium and sterilize it by flash steaming until it is half cooked; S2: Sterilized bagged culture medium inoculated with liquid bacteria; S3: Waiting for the inoculated culture medium to be full of hyphae; Making Grifola frondosa log mushroom sticks The pretreated culture medium is unpacked and placed in the hollow part of the pretreated chestnut log, and both ends are sealed to form a Grifola frondosa log mushroom stick.

[0010] Preferably, in step S3 of the pretreatment process of the chestnut logs, the spacing between the holes on the outer wall of the chestnut logs is 15-20 cm.

[0011] Preferably, in step S4 of the pretreatment process of the chestnut logs, the treatment liquid is a white rot fungus suspension, and the spraying dosage is 15-20 ml / dm 2 After spraying the treatment liquid, the chestnut logs should be placed in a dark, ventilated and moist place for 60-75 days.

[0012] Preferably, in step S5 of the pretreatment process of the chestnut logs, the formula of the white rot fungus suspension is: the spore density of Phanerochaete chrysosporium is not less than 10 6 / ml, glucose content is 2%, sodium carboxymethyl cellulose 0.8%, pH value is 4-5.

[0013] Preferably, the culture medium comprises the following components in weight ratio: Chestnut sawdust 20%, cottonseed husk 60%, soybean meal 8%, raw soil 6%, gypsum 2%, sugar 2%, pH 6.0-7.0; In the pretreatment process step S1 of the culture medium, water is added to the culture medium for mixing, and then the culture medium is bagged for flash sterilization, and the weight ratio of the culture medium to water is 1:1.1; In step S2 of the pretreatment process of the culture medium, the density of the inoculated liquid bacteria is greater than 1.15 g / ml, and the inoculation amount is 10 ml / Kg.

[0014] Beneficial effects of the present invention: The invention optimizes the mushroom sticks and growth pattern of Grifola frondosa, provides a sustainable and stable Grifola frondosa cultivation method, can simulate the growth pattern of wild Grifola frondosa, and the mushroom sticks can achieve continuous mushroom production for many years, thereby improving the nutritional value of Grifola frondosa, increasing the yield of Grifola frondosa, reducing the production cost of enterprises, and increasing economic benefits. DETAILED DESCRIPTION

[0015] The specific implementation modes of the present invention are further described below in conjunction with examples. The following examples are only used to more clearly illustrate the technical solutions of the present invention, and are not intended to limit the protection scope of the present invention.

[0016] A Grifola frondosa log mushroom stick comprises a culture vessel and culture medium; the culture vessel is a hollow chestnut log, and the culture medium is placed in the hollow position of the chestnut log.

[0017] An application of Grifola frondosa log mushroom sticks, wherein the Grifola frondosa log mushroom sticks are cultivated and grown using any one of the following cultivation modes: a simulated wild soil covering mode, a layer rack mode, and a layer rack soil covering coupling mode. The simulated wild soil covering mode is specifically: grooves are made on the ground under a chestnut forest, and the Grifola frondosa log mushroom sticks are evenly placed in the grooves. After the arrangement, the surface is covered with 2-5 cm of raw soil, and water is irrigated once every 5-10 days, and mushrooms can be grown continuously for 3-5 years. The layer rack mode is specifically: the Grifola frondosa log mushroom sticks are evenly placed on the mushroom racks in a greenhouse, and the temperature and humidity in the greenhouse are controlled and ventilated. After 50-75 days, mushrooms are grown at the mushroom hole position, and mushrooms can be grown continuously for 3-5 tides. The rack-covering coupling method is as follows: the mushroom outlet of the Grifola frondosa log mushroom sticks after mushroom production in the rack mode is drilled again with a clean drill bit to the internal material replacement position and debris is cleaned up, the liquid spawn is inoculated for a second time at the drilled hole, and the hole is sealed with absorbent cotton after inoculation; the Grifola frondosa log mushroom sticks after the second inoculation are expelled and cultivated in a simulated wild soil covering mode, and are irrigated with water once every 5-10 days, so that mushrooms can be produced continuously for 2-4 years.

[0018] A method for making a Grifola frondosa log mushroom stick comprises the following steps: Pretreatment of chestnut logs S1: Select appropriate chestnut logs; S2: removing the central wood core of the chestnut log; S3: drilling a hole on the outer wall of the chestnut log to connect with the hollow part of the chestnut log; S4: Spraying the surface of the chestnut log with the treatment liquid; S5: flash sterilizing the treated chestnut logs for later use; Pretreatment of culture medium S1: Bag the culture medium and sterilize it by flash steaming until it is half cooked; S2: Sterilized bagged culture medium inoculated with liquid bacteria; S3: Waiting for the inoculated culture medium to be full of hyphae; Making Grifola frondosa log mushroom sticks The pretreated culture medium is unpacked and placed in the hollow part of the pretreated chestnut log, and both ends are sealed to form a Grifola frondosa log mushroom stick. As a preferred solution, both ends of the Grifola frondosa log mushroom stick are sealed with polyethylene film and brushed with lime water.

[0019] As a preferred solution, in the chestnut log pretreatment step S1, the chestnut log is dried chestnut wood, and a straight part with a diameter of 15-40 cm and a length of 20-80 cm is taken; in step S2, the wood core diameter of the center of the log is removed by 7-20 cm, and the chestnut wood layer thickness of 4-10 cm is retained to make the log hollow; in the chestnut log pretreatment process step S3, the spacing of the holes on the outer wall of the chestnut log is 15-20 cm, and as a preferred solution, the hole diameter is 2 cm. In the chestnut log pretreatment process step S4, the treatment liquid is a white rot fungus suspension, and the spraying dosage is 15-20 ml / dm 2 After spraying the treatment solution, the chestnut logs are placed in a dark, ventilated and moist place for 60-75 days. Step S4 can be repeated once according to the specific situation. Preferably, the pretreatment temperature of the chestnut logs is preferably above 10°C. When the lowest temperature of the day is below 10°C, the treatment time can be appropriately extended. In step S5 of the pretreatment process of the chestnut logs, the formula of the white rot fungus suspension is: the spore density of Phanerochaete chrysosporium Burdsall is not less than 10 6 / ml, glucose content is 2%, sodium carboxymethyl cellulose 0.8%, pH value is 4-5.

[0020] The culture medium includes the following components in weight ratio: 20% chestnut sawdust, 60% cottonseed husk, 8% soybean meal, 6% raw soil, 2% gypsum, 2% sugar, and a pH value of 6.0-7.0; in step S1 of the pretreatment process of the culture medium, the culture medium is mixed with water and then bagged for flash sterilization, and the weight ratio of the culture medium to water is 1:1.1; in step S2 of the pretreatment process of the culture medium, the density of the inoculated liquid bacteria is greater than 1.15g / ml, and the inoculation amount is 10ml / Kg.

[0021] Obviously, the above embodiments are only examples for the purpose of clearly explaining the invention, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the scope of protection of the invention.

Claims

1. A Grifola frondosa log mushroom stick, characterized in that: Including culture vessels and culture media; The culture vessel is a hollow chestnut log, and the culture material is placed in the hollow position of the chestnut log.

2. The use of a Grifola frondosa log mushroom stick as claimed in claim 1, wherein the Grifola frondosa log mushroom stick is cultivated and grown in any one of a simulated wild soil covering mode, a layer rack mode, and a layer rack soil covering coupled mode.

3. The use of the Grifola frondosa log mushroom stick according to claim 2, characterized in that: The simulated wild soil covering mode is specifically as follows: Dig grooves on the ground under the chestnut forest, place the Grifola frondosa logs evenly in the grooves, and cover the surface with 2-5 cm of raw soil after arranging them.

4. The use of the Grifola frondosa log mushroom stick according to claim 2, characterized in that: The shelf mode is specifically: In the greenhouse, the raw wood mushroom sticks are evenly placed on the mushroom racks. The temperature and humidity in the greenhouse are controlled and ventilated. Mushrooms will grow at the mushroom holes after 50-75 days.

5. The use of the Grifola frondosa log mushroom stick according to claim 2, characterized in that: The layer frame soil covering coupling type is specifically: The fruiting port of the Grifola frondosa log fruiting stick after fruiting in the shelf mode is drilled again with a clean drill bit to the internal substitute material and the debris is cleaned, and the liquid strain is inoculated at the drilled hole for the second time, and the hole is sealed with absorbent cotton after the strain is inoculated; The Grifola frondosa logs after the second inoculation are used for excretion and cultivation in a simulated wild soil covering mode.

6. The method for making a Grifola frondosa log mushroom stick according to claim 1, characterized in that: The following steps are involved: Pretreatment of chestnut logs S1: Select appropriate chestnut logs; S2: removing the central wood core of the chestnut log; S3: drilling a hole on the outer wall of the chestnut log to connect with the hollow part of the chestnut log; S4: Spraying the surface of the chestnut log with the treatment liquid; S5: flash sterilizing the treated chestnut logs for later use; Pretreatment of culture medium S1: Bag the culture medium and sterilize it by flash steaming until it is half cooked; S2: Sterilized bagged culture medium inoculated with liquid bacteria; S3: Waiting for the inoculated culture medium to be full of hyphae; Making Grifola frondosa log mushroom sticks The pretreated culture medium is unpacked and placed in the hollow part of the pretreated chestnut log, and both ends are sealed to form a Grifola frondosa log mushroom stick.

7. The method for making a Grifola frondosa log mushroom stick according to claim 6, characterized in that: In step S3 of the pretreatment process of the chestnut logs, the spacing between the holes on the outer wall of the chestnut logs is 15-20 cm.

8. The method for making a Grifola frondosa log mushroom stick according to claim 6, characterized in that: In the pretreatment process step S4 of the chestnut log, the treatment liquid is a white rot fungus suspension, and the spraying dosage is 15-20 ml / dm 2 After spraying the treatment liquid, the chestnut logs should be placed in a dark, ventilated and moist place for 60-75 days.

9. The method for making a Grifola frondosa log mushroom stick according to claim 6, characterized in that: In the step S5 of the pretreatment process of the chestnut logs, the formula of the white rot fungus suspension is: the spore density of Phanerochaete chrysosporium is not less than 10 6 / ml, glucose content is 2%, sodium carboxymethyl cellulose 0.8%, pH value is 4-5.

10. The method for making a Grifola frondosa log mushroom stick according to claim 6, characterized in that: The culture medium comprises the following components in weight ratio: Chestnut sawdust 20%, cottonseed husk 60%, soybean meal 8%, raw soil 6%, gypsum 2%, sugar 2%, pH 6.0-7.0; In the pretreatment process step S1 of the culture medium, water is added to the culture medium for mixing, and then the culture medium is bagged for flash sterilization, and the weight ratio of the culture medium to water is 1:1.1; In step S2 of the pretreatment process of the culture medium, the density of the inoculated liquid bacteria is greater than 1.15 g / ml, and the inoculation amount is 10 ml / Kg.