Cultivation method for enriching lithium element of cardamine violifolia

Abstract

The invention relates to the field of agricultural planting, in particular to a cultivation method for enriching lithium elements of cardamine violifolia. The cultivation method comprises the following steps: (1) seedling raising: sowing cardamine violifolia seeds in a substrate for seedling raising; (2) transplanting: when the cardamine violifolia seedlings grow to have 4-5 true leaves, transplanting the cardamine violifolia seedlings into a water culture box filled with clear water; 3 days after transplanting, replacing clean water in the water culture box with a nutrient solution with the concentration of 1 / 8, adding exogenous selenium, and then replacing the nutrient solution and the exogenous selenium once every 10 days; 45-60 days after transplanting, a nutrient solution with the concentration being 1 / 4 is replaced; a nutrient solution with the concentration being 1 / 4 is used for water culture for 2 days, then a Li source is added into the culture solution, water culture continues for 6-12 days, and then harvesting is conducted. According to the method, the lithium source is added into the culture solution, so that rhizomes and leaves of cardamine violifolia are enriched with the lithium element, and a safer raw material source is provided for producing biological lithium-rich active ingredients.

Description

Technical Field

[0001] The invention relates to the field of agricultural planting, and in particular to a cultivation method for Cardamine violata to enrich lithium elements. Background Art

[0002] Cardamine violifolia belongs to the genus Cardamine in the family Cruciferae. It is mainly distributed in Enshi, Hubei and Hupingshan, Hunan. It is a perennial plant. Its tender stems and leaves are edible. They are fresh and delicious. It is a wild vegetable resource for local villagers. Cardamine violifolia has strong tolerance and enrichment ability for trace elements, especially selenium.

[0003] Lithium is a chemical element that has beneficial effects on the human body, including the treatment of bipolar disorder, prevention of cognitive deterioration, anti-inflammatory effects, and effects on cardiovascular health. Lithium salts are a common drug for the treatment of bipolar disorder, which can relieve symptoms such as mood swings, insomnia, anxiety, etc. Studies have shown that lithium salts help prevent cognitive deterioration in the elderly and neurodegenerative diseases such as Alzheimer's disease. Lithium also has an inhibitory effect on inflammation and can alleviate the symptoms of some inflammatory diseases. Lithium can lower blood pressure, improve heart health, and has a preventive effect on cardiovascular disease. However, most medical lithium salts are currently synthesized from industrial raw material lithium after purification, and there are no reports of biogenic lithium raw materials. Moreover, lithium is not an essential nutrient for plants, and improper concentrations can easily lead to plant poisoning.

[0004] Currently, Cardamine violeta is cultivated as a selenium-rich crop, and is produced on a large scale using soil or substrate cultivation. There are no reports on hydroponics and lithium enrichment. Therefore, developing a method for enriching lithium in Cardamine violeta with accurate quantitative results and less soil pollution has become a technical problem that needs to be solved urgently in this field. Summary of the invention

[0005] Based on the above content, the present invention provides a cultivation method for Cardamine violata to enrich lithium elements.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a cultivation method for Cardamine violata to enrich lithium, comprising the following steps:

[0008] (1) Seedling cultivation: sowing Cardamine violaceum seeds in a substrate for seedling cultivation;

[0009] (2) Transplanting: When the Cardamine viola seedlings grow to 4-5 true leaves, transplant the Cardamine viola seedlings into a hydroponic box filled with clean water;

[0010] Three days after transplanting, replace the water in the hydroponic tank with a 1 / 8 concentration nutrient solution and add exogenous selenium. Then replace the nutrient solution and exogenous selenium every 10 days; 45-60 days after transplanting, replace the 1 / 4 concentration nutrient solution;

[0011] After 2 days of hydroponics with a 1 / 4 concentration nutrient solution, a Li source is added to the culture solution, and the culture is continued for 6-12 days before harvesting, thereby completing the lithium enrichment cultivation of Cardamine violacea.

[0012] In a preferred embodiment of the present invention, in step (1), the environment temperature for raising seedlings is 18±1° C., and the relative humidity is 70%-80%.

[0013] In a preferred embodiment of the present invention, in step (1), the lighting conditions for the seedling cultivation are: light 100 μmol / m 2 / s, day / night is 14 / 10 hours.

[0014] In a preferred embodiment of the present invention, in step (2), the temperature after transplanting is 22±1°C day / 16±1°C night, and the relative humidity of the environment is 70%-80%.

[0015] In a preferred embodiment of the present invention, in step (2), the light conditions after transplanting are: 200 μmol / m 2 / s, day / night is 16 / 8 hours.

[0016] In a preferred embodiment of the present invention, in step (2), the nutrient solution is Hoagland nutrient solution.

[0017] In a preferred embodiment of the present invention, the exogenous selenium is sodium selenite; the concentration of the exogenous selenium in the 1 / 8 concentration nutrient solution is 1.5 μmol / L.

[0018] In a preferred embodiment of the present invention, the Li source is LiCl; the concentration of the Li source in the 1 / 4 concentration nutrient solution is 50-200 mg / L; further preferably, it is 200 mg / L.

[0019] In a preferred embodiment of the present invention, the hydroponic culture is continued for 9 days after the Li source is added in step (2).

[0020] The present invention discloses the following technical effects:

[0021] The invention utilizes the characteristic of Cardamine violacea that it is easy to enrich trace elements, and enriches the rhizomes and leaves of Cardamine violacea with lithium elements by adding a lithium source into the culture solution, thereby providing a safer raw material source for producing biological lithium-rich active ingredients.

[0022] The cultivation method of the invention not only enables the Cardamine violaceae to grow vigorously, but also significantly improves the total lithium content of the Cardamine violaceae, thereby increasing the nutritional value and commercial value of the Cardamine violaceae.

[0023] The present invention utilizes hydroponics to carry out lithium-rich cultivation of Cardamine violaceae, has the advantages of simple operation, low cost, labor and material saving, simple operation, little residue, high application value for large-scale planting of lithium-rich Cardamine violaceae, and can be used for large-scale production of lithium-rich Cardamine violaceae. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 It is the fresh weight of Cardamine violacea grown in water with different concentrations of Li in Example 1 for 12 days.

[0026] Figure 2 The total lithium content in the aboveground part and root system of Cardamine violacea grown in water with different concentrations of Li for 12 days in Example 1.

[0027] Figure 3 The lithium content of different aboveground cell components of Cardamine violacea grown in water with different concentrations of Li for 12 days in Example 1 is shown.

[0028] Figure 4 The aboveground dissolved lithium contents of Cardamine violacea grown in water with different concentrations of Li for 12 days in Example 1 are shown.

[0029] Figure 5 The fresh weight of Cardamine violacea cultured in 200 mg / L Li water at different growth days in Example 2.

[0030] Figure 6 The total lithium content of the aboveground part and the root system of Cardamine violacea at different growth days when cultured in 200 mg / L Li water in Example 2.

[0031] Figure 7 The lithium content of different cell components in the aboveground part of Cardamine violacea cultured in 200 mg / L Li water at different growth days in Example 2. DETAILED DESCRIPTION

[0032] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0033] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0034] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0035] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0036] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0037] The present invention utilizes a hydroponic method, uses a nutrient solution with added exogenous selenium to first cultivate Cardamine violaceae, and then adds a lithium source to the nutrient solution after the Cardamine violaceae grows to a certain stage. A suitable hydroponic concentration for lithium-rich cultivation of Cardamine violaceae seedlings is screened out, and then a complete hydroponic method for Cardamine violaceae is obtained. Utilizing the cultivation method of the present invention, not only does Cardamine violaceae grow vigorously, but also the total lithium content of Cardamine violaceae is significantly improved, thereby increasing the nutritional value and commercial value of Cardamine violaceae.

[0038] The embodiment of the present invention provides a cultivation method for Cardamine violata to enrich lithium, comprising the following steps:

[0039] (1) Seedling cultivation: sowing Cardamine violaceum seeds in a substrate for seedling cultivation;

[0040] (2) Transplanting: When the Cardamine viola seedlings grow to 4-5 true leaves, transplant the Cardamine viola seedlings into a hydroponic box filled with clean water;

[0041] Since Cardamine violeta is a super-selenium-accumulating plant, a certain amount of selenium can effectively promote the healthy growth of Cardamine violeta and improve its stress resistance. Therefore, 3 days after transplanting, replace the clean water in the hydroponic box with a 1 / 8 concentration nutrient solution and add exogenous selenium at the same time. After that, replace the nutrient solution and exogenous selenium every 10 days. This step is very important for subsequent Li-rich cultivation; 45-60 days after transplanting, replace the 1 / 4 concentration nutrient solution;

[0042] After 2 days of hydroponics with 1 / 4 concentration nutrient solution, add Li source to the culture solution. Since Li is not an essential nutrient element for plants and can easily cause toxicity to plants, it must be added at the right time. At this time, Cardamine violae is growing vigorously, has strong resistance to adversities, and has strong root activity. After adding exogenous Li, it can not only absorb and transform quickly, accumulate in the leaves, but also continue to grow healthily. If exogenous Li is added too early, the plant will be small and have poor resistance, and it is easy to produce toxicity; if it is added too late, the root activity will decrease, and it will not be easy to enrich a high content of Li. After adding exogenous Li, continue hydroponics for 6-12 days before harvesting, and the lithium enrichment cultivation of Cardamine violae is completed.

[0043] The technical solutions described in the present invention, unless otherwise specified, are all conventional solutions in the art, and the reagents or raw materials used, unless otherwise specified, are purchased from commercial channels or have been disclosed.

[0044] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0045] Example 1

[0046] Seedling cultivation of Cardamine violae: Seedling cultivation was carried out in a 32-hole plug tray with river sand. On July 23, 2023, Cardamine violae seeds were sown on the plug tray with river sand. The seedling cultivation environment temperature was 18°C, the relative humidity was 70%-80%, and the light intensity was 100 μmol / m 2 / s, day and night are 14 hours / 10 hours.

[0047] Transplantation: When the seedlings of Cardamine violae grow to four to five true leaves, carefully remove the seedlings from the plug tray on September 9, 2023, wash the river sand on the roots, transplant them into the hydroponic box, and fix them in the planting basket with a sponge. At this time, the hydroponic box is filled with clean water without adding nutrient solution to allow the transplanted seedlings to adapt to the hydroponic environment. At the same time, an aeration pump is placed in the hydroponic box (the air output is 1.5 liters / minute, and the ventilation is 14 hours a day, which is consistent with the light cycle. The light condition after transplantation is 200μmol / m 2 / s, day / night is 16 / 8 hours. ).

[0048] Three days after transplanting, the water in the hydroponic tank was replaced with 1 / 8 Hoagland nutrient solution, and sodium selenite was added to make the final concentration of 1.5 μmol / L, and the nutrient solution and sodium selenite were updated every 10 days; 45 days after transplanting, it was replaced with 1 / 4 Hoagland nutrient solution, and sodium selenite was no longer added; after continuing hydroponics for 2 days, LiCl was added to the culture solution, and the final concentrations of LiCl were 0 mg / L, 50 mg / L, 100 mg / L, 200 mg / L, and 400 mg / L, respectively, and the water was harvested after continuing hydroponics for 12 days, thus completing the lithium enrichment cultivation of Cardamine violacea.

[0049] Figure 1 The fresh weight of Cardamine violacea cultured in water at different concentrations of LiCl is shown in the figure (in the figure, CK means that the final concentration of LiCl is 0 mg / L, Li 50 means that the final concentration of LiCl is 50 mg / L, and so on, Li 400 means that the final concentration of LiCl is 400 mg / L, the same below). The results show that 50-200 mg / L LiCl has no significant effect on the growth of Cardamine violacea, while 400 mg / L significantly inhibits its growth.

[0050] Figure 2 The total lithium content in the aerial parts and roots of Cardamine violae cultured in water with different concentrations of LiCl is shown. Figure 2 It can be seen that the Li absorbed by Cardamine violaceae mainly accumulates in the aboveground part, and less Li accumulates in the root system. At the same time, with the increase of LiCl concentration, the Li content in Cardamine violaceae also increases significantly. The Li content of the aboveground part of Cardamine violaceae treated with 100 mg / L and 200 mg / L LiCl is relatively close, and both exceed 100 mg / kg fresh weight.

[0051] Figure 3 The figure shows the lithium content of different cell components in the aerial part of Cardamine violacea cultured in water with different concentrations of Li. It can be seen from the figure that the Li of Cardamine violacea aerial part is mainly accumulated in the cell wall and soluble components. This shows that the Li of Cardamine violacea should be mainly extracted biologically from the cell wall and soluble components.

[0052] Figure 4 The figure shows the different dissolved lithium contents in the aboveground parts of Cardamine violae in water cultured with different concentrations of Li. As can be seen from the figure, the Li in the aboveground parts of Cardamine violae mainly exists in alcohol-soluble (Li-ethanol) and water-soluble (Li-H2O) substances. This shows that the Li of Cardamine violae should be mainly extracted using ethanol and water.

[0053] Example 2

[0054] According to the results of Example 1, 200 mg / L LiCl is the preferred concentration. Based on this result, Example 2 was carried out.

[0055] Seedling cultivation of Cardamine violae: Seedling cultivation was carried out in a 32-hole plug tray with river sand. On March 10, 2024, Cardamine violae seeds were sown on the plug tray with river sand. The seedling cultivation environment temperature was 18°C, the relative humidity was 70%-80%, and the light intensity was 100 μmol / m 2 / s, day and night are 14 hours / 10 hours.

[0056] Transplantation: When the seedlings of Cardamine violae grow to four to five true leaves, carefully remove the seedlings from the plug tray on April 21, 2024, wash the river sand on the roots, transplant them into the hydroponic box, and fix them in the planting basket with a sponge. At this time, the hydroponic box is filled with clean water without adding nutrient solution to allow the transplanted seedlings to adapt to the hydroponic environment. At the same time, an aeration pump is placed in the hydroponic box (the air output is 1.5 liters / minute, and the ventilation is 14 hours a day, which is consistent with the light cycle. The light condition after transplantation is 200μmol / m 2 / s, day / night is 16 / 8 hours).

[0057] Three days after transplanting, the water in the hydroponic tank was replaced with 1 / 8 Hoagland nutrient solution, and sodium selenite was added to make the final concentration of 1.5μmol / L. After that, the nutrient solution and sodium selenite were updated every 10 days; 45 days after transplanting, it was replaced with 1 / 4 Hoagland nutrient solution, and sodium selenite was no longer added; after continuing hydroponic cultivation for 2 days, LiCl was added to the culture solution to make the final concentration of LiCl 200mg / L. Continue hydroponic cultivation and harvest in batches on the 3rd, 6th, 9th and 12th days after adding Li. The Li accumulation of Cardamine violacea at different growth times was investigated at the optimal concentration.

[0058] Figure 5 The figure shows the fresh weight of Cardamine violae at different growth days when cultivated in 200 mg / L Li water. As can be seen from the figure, Cardamine violae can continue to grow in the nutrient solution with this concentration of Li, and its fresh weight is the largest on the 9th day.

[0059] Figure 6 The total lithium content of the aboveground and root system of Cardamine violacea cultivated with 200 mg / L Li water at different growth days. As can be seen from the figure, with the extension of the cultivation time, the Li content of the aboveground and root system of Cardamine violacea increased significantly, and the content of the aboveground part was significantly higher than that of the root system. And there was a considerable content on the 9th day.

[0060] Figure 7 The lithium content of different cell components in the aerial part of Cardamine violaceum was measured by culturing Cardamine violaceum in 200 mg / L Li water. As in Example 1, the Li in the aerial part of Cardamine violaceum was mainly accumulated in the cell wall and soluble components.

[0061] As can be seen from the above results, the present invention uses a hydroponic method to pre-cultivate Cardamine violae with a selenium-containing nutrient solution to make it grow healthily, and lithium is added to the nutrient solution after it grows to a certain stage to enrich it with lithium. The appropriate hydroponic concentration and harvesting time for lithium-rich cultivation of Cardamine violae seedlings are screened out, and then a complete method for hydroponic cultivation of Cardamine violae is obtained; using the cultivation method in this application, the total lithium content of Cardamine violae is significantly improved, and the nutritional value and commercial value of Cardamine violae are increased. Using a hydroponic method to cultivate Cardamine violae for lithium enrichment is simple to operate, low in cost, labor-saving and material-saving, easy to operate, and less residual. It has a high application value for the large-scale planting of lithium-rich Cardamine violae, and can be used for large-scale production of lithium-rich Cardamine violae.

[0062] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A cultivation method for Cardamine violata to enrich lithium, characterized in that: The following steps are involved: (1) Seedling cultivation: sowing Cardamine violaceum seeds in a substrate for seedling cultivation; (2) Transplanting: When the Cardamine viola seedlings grow to 4-5 true leaves, transplant the Cardamine viola seedlings into a hydroponic box filled with clean water; Three days after transplanting, replace the water in the hydroponic tank with a 1 / 8 concentration nutrient solution and add exogenous selenium. Then replace the nutrient solution and exogenous selenium every 10 days; 45-60 days after transplanting, replace the 1 / 4 concentration nutrient solution; After 2 days of hydroponics with a 1 / 4 concentration nutrient solution, a Li source is added to the culture solution, and the culture is continued for 6-12 days before harvesting, thereby completing the lithium enrichment cultivation of Cardamine violacea.

2. The cultivation method according to claim 1, characterized in that In step (1), the environment temperature for raising seedlings is 18±1° C. and the relative humidity is 70%-80%.

3. The cultivation method according to claim 1, characterized in that In step (1), the lighting conditions for the seedling cultivation are: light 100 μmol / m 2 / s, day / night is 14 / 10 hours.

4. The cultivation method according to claim 1, characterized in that: In step (2), the temperature after transplanting is 22±1°C day / 16±1°C night, and the relative humidity of the environment is 70%-80%.

5. The cultivation method according to claim 1, characterized in that: In step (2), the light conditions after transplanting are: 200 μmol / m 2 / s, day / night is 16 / 8 hours.

6. The cultivation method according to claim 1, characterized in that: In step (2), the nutrient solution is Hoagland nutrient solution.

7. The cultivation method according to claim 1, characterized in that: The exogenous selenium is sodium selenite; the concentration of the exogenous selenium in the 1 / 8 concentration nutrient solution is 1.5 μmol / L.

8. The cultivation method according to claim 1, characterized in that: The Li source is LiCl; the concentration of the Li source in the 1 / 4 concentration nutrient solution is 50-200 mg / L.

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