Vinasse yellow water feed and application thereof in breeding of hermetia illucens

Abstract

The invention relates to the technical field of hermetia illucens breeding, and particularly discloses vinasse yellow water feed and application thereof in hermetia illucens breeding. The vinasse yellow water feed comprises the following raw materials in parts by weight: 30-50 parts of vinasse, 5-10 parts of yellow water, 4-8 parts of a plant extract, 1-2 parts of beta-cyclodextrin and 100-200 parts of a basal feed, the basal feed comprises bran, bagasse, pineapple residues and soybean curb residues in a mass ratio of 10: (2-5): (1-7): (3-9); the plant extract is obtained by extracting mangnolia officinalis, scutellaria baicalensis and rosemary as raw materials; the obtained vinasse yellow water feed is used for breeding the hermetia illucens, plant protein is effectively converted into high-value animal and insect protein, larvae of the hermetia illucens are rich in high-quality protein and fat, the content of crude protein reaches 42% or above, the storage problem of the vinasse yellow water is solved, efficient utilization of resources is achieved, and the vinasse yellow water feed has wide market prospects.

Description

Technical Field

[0001] The present application relates to the technical field of black soldier fly breeding, and more specifically, to a vinasse yellow water feed and its application in black soldier fly breeding. Background Art

[0002] As a common insect, black soldier fly has high nutritional value and rich protein and fat content. It is a kind of soldier fly insect that can be produced as a resource. The larvae of black soldier fly have many advantages such as a wide range of food, fast growth and development, and strong adaptability. While ensuring their own growth and development, they can also maintain a high waste degradation rate. Its degradation and transformation mainly occurs in the larval stage of black soldier fly, converting organic waste into valuable biomass with less impact on the environment and lower greenhouse gas emissions. It has been proven to have important uses in the treatment of poultry and livestock manure and domestic waste, the production of animal protein feed, and the preparation of biodiesel.

[0003] At present, CN112741234A discloses a black soldier fly efficient breeding feed composed of the following components in parts by weight: 70-90 parts of potato residue, 5-10 parts of hemp residue and 0-15 parts of straw shreds; a special fermentation agent is sprayed on the surface of the formula material, and 300-700g of bacterial liquid is sprayed on every 100kg of the formula material for aerobic fermentation; the special fermentation agent is composed of the following fermentation liquid in parts by weight: 20-60 parts of Candida utilis, 20-60 parts of Bacillus natto and 20-60 parts of Aspergillus niger; the potato residue in the formula is used to provide carbohydrates, and the hemp residue can effectively increase the protein and fat content in the formula to reach a level suitable for black soldier flies to feed; the straw shreds make the material relatively fluffy, increase the air permeability of the material, adjust the moisture content of the material, improve the feeding efficiency of black soldier flies, and thus significantly improve the average yield of black soldier flies. However, a large amount of organic waste will affect the efficiency of bacterial fermentation, making it difficult to provide a good growth environment for black soldier flies. Black soldier flies cannot resist the invasion of pathogenic microorganisms, which will affect their survival rate and growth rate.

[0004] Therefore, it is urgent to propose a distiller's grains yellow water feed and its application in black soldier fly breeding to ensure the normal growth and development of black soldier flies and increase the yield of black soldier flies. Summary of the invention

[0005] In order to solve the problem of poor survival rate of existing black soldier flies, the present application provides a distiller's grains yellow water feed and its application in black soldier fly breeding.

[0006] In the first aspect, the present application provides a vinasse yellow water feed, which adopts the following technical solution:

[0007] A distiller's grains and yellow water feed comprises the following raw materials in parts by weight: 30-50 parts of distiller's grains, 5-10 parts of yellow water, 4-8 parts of plant extracts, 1-2 parts of beta-cyclodextrin, and 100-200 parts of basic feed.

[0008] Preferably, the basic feed comprises bran, bagasse, pineapple residue and bean curd residue in a mass ratio of 10:(2-5):(1-7):(3-9).

[0009] Preferably, the method for preparing the plant extract comprises the following steps:

[0010] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary, crushing and passing through an 80-100 mesh sieve, adding 2-3 times of the total mass of water, heating to 60-75° C., keeping warm for 30-60 minutes, cooling to 5-10° C., soaking for 3-5 hours, adding ethanol to make the concentration of ethanol in the mixture 60-75%, and precipitating at 14-20° C. for 3-6 hours to obtain an extract and a residue;

[0011] Step (2) adding 10-15 times the mass of the residue of water and 0.1-0.3 times the mass of the residue of the composite bacteria to the residue for fermentation at a temperature of 30-35° C. for 10-13 h, sterilizing, filtering, and obtaining a fermentation liquid;

[0012] Step (3) The extract is mixed with the fermentation liquid and concentrated to obtain a plant extract.

[0013] Preferably, the mass ratio of Magnolia officinalis, Scutellaria baicalensis and Rosemary is (1.4-1.7):1:(0.2-0.5).

[0014] Preferably, the composite bacterial species include Bacillus subtilis, Candida albicans and cellulose decomposing bacteria.

[0015] Preferably, the concentration of Bacillus subtilis is (1-1.8)×10 6 CFU / mg, the concentration of the Candida albicans is (0.5-1.2)×10 6 CFU / mg, the concentration of the cellulose decomposing bacteria is (1-2)×10 6 CFU / mg.

[0016] Preferably, the concentration ratio is 1:(1-3).

[0017] Preferably, the yellow water is pretreated by the following method:

[0018] Take yellow water, filter and remove impurities, adjust the pH to 5-5.5 with feed-grade sodium hydroxide, and then pass it through a distillation system to increase the solid content of the yellow water to 20-40%, with a concentration ratio of 1:(2-5).

[0019] In the second aspect, the present application provides a method for preparing vinasse yellow water feed, which adopts the following technical scheme:

[0020] A method for preparing vinasse yellow water feed comprises the following steps:

[0021] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0022] In a third aspect, the present application provides an application of distiller's grains yellow water feed in black soldier fly breeding, using the following technical solution:

[0023] Application of distiller's grains yellow water feed in black soldier fly breeding comprises the following steps:

[0024] S1, wheat bran, chicken feed and water are mixed and stirred in a mass ratio of 20:(5-15):100, and black soldier fly egg masses are placed for cultivation to obtain primary culture larvae;

[0025] S2. Placing the initially cultured larvae in the above-mentioned vinasse yellow water feed for cultivation to obtain black soldier fly larvae.

[0026] Preferably, the culture conditions in S1 are: temperature of 26-30°C, humidity of 60-80%, and time of 4-6 days; the culture conditions in S2 are: temperature of 26-30°C, humidity of 60-80%, and time of 7-10 days.

[0027] In summary, this application has the following beneficial effects:

[0028] 1. The vinasse yellow water feed of the present application includes vinasse, yellow water, plant extracts, β-cyclodextrin, and basic feed, and the basic feed includes bran, bagasse, pineapple residue, and tofu residue; vinasse contains rich nutrients such as protein, fat, carbohydrates and minerals, which are conducive to the growth and development of black soldier flies; yellow water, as a by-product in the winemaking process, also contains certain nutrients, which can further enrich the nutritional structure of the feed; plant extracts can provide a variety of bioactive substances, which are conducive to enhancing the immunity and growth performance of black soldier flies; β-cyclodextrin not only has the functions of embedding and sustained release, which improves the utilization rate of feed, but also can be used as a stabilizer to effectively ensure that the nutrients in the feed will not deteriorate; the basic feed (bran, bagasse, pineapple residue, tofu residue) provides rich carbohydrates and cellulose, which is conducive to adjusting the overall structure of the feed, making the feed easier to be digested and absorbed by black soldier flies; the components in the vinasse yellow water feed of the present application interact with each other, and the nutrition is rich and balanced, which can meet the various nutritional needs of black soldier flies during their growth, thereby promoting their rapid growth and development.

[0029] 2. The plant extract of the present application uses Magnolia officinalis, Scutellaria baicalensis and Rosemary as raw materials. The interaction between the three can inhibit the growth of pathogenic microorganisms and reduce the occurrence of diseases such as bacteria and fungi, thereby improving the sanitary conditions of the farm, reducing the spread of diseases in the breeding environment of black soldier flies, and maintaining a good living environment; at the same time, the active ingredients in the plant extract have a promoting effect on the immune system of black soldier flies, enhance their resistance, and reduce the occurrence of diseases. In addition, in the preparation process of the plant extract of the present application, ethanol is first used for alcohol extraction, and then fermented with a composite strain, which extracts the active ingredients of the plant to a great extent, and the fermentation process adds biologically active factors, so that the final plant extract can not only effectively improve the breeding environment, enhance the disease resistance of black soldier flies, and promote their healthy growth, but also improve their reproductive capacity and survival rate.

[0030] 3. This application pre-treats the yellow water and adjusts the pH of the yellow water to ensure that the pH of the yellow water is suitable for the growth of black soldier flies; concentrated yellow water helps to increase the nutritional density, improve the efficiency of breeding, reduce the waste of water and waste, and thus improve production efficiency. The black soldier flies using this feed have a higher yield and better quality.

[0031] 4. This application uses distiller's grains and yellow water feed to breed black soldier flies, effectively converting plant protein into high-value animal insect protein; using byproducts of distiller's grains and yellow water not only solves the storage problem, but also achieves efficient utilization of resources, realizes the reuse of agricultural waste, reduces waste, and promotes industrial symbiosis. At the same time, strict breeding management ensures the safety and hygiene of distiller's grains and yellow water feed, and black soldier fly larvae can neutralize harmful bacteria, reduce the risk of disease transmission, and increase the survival rate of black soldier fly larvae. DETAILED DESCRIPTION

[0032] The present application is further described in detail below with reference to the embodiments.

[0033] Embodiment 1-3 provides a vinasse yellow water feed and a preparation method thereof.

[0034] Example 1

[0035] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0036] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0037] The method for preparing the plant extract comprises the following steps:

[0038] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary in a mass ratio of 1.4:1:0.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract and a residue;

[0039] Step (2) adding water 10 times the mass of the residue and a composite bacterial strain 0.1 times the mass of the residue to the residue for fermentation at a fermentation temperature of 30° C. for a fermentation time of 10 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1×10 6 CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6 CFU / mg of cellulose decomposing bacteria;

[0040] Step (3) after mixing the extract and the fermentation liquid, concentrating the mixture at a concentration ratio of 1:1 to obtain a plant extract;

[0041] Yellow water is pretreated by the following methods:

[0042] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0043] A method for preparing vinasse yellow water feed comprises the following steps:

[0044] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0045] Example 2

[0046] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 40 parts of distiller's grains, 8 parts of yellow water, 6 parts of plant extract, 1.5 parts of beta-cyclodextrin, and 150 parts of basic feed;

[0047] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:4:5:6;

[0048] The method for preparing the plant extract comprises the following steps:

[0049] Step (1) Magnolia officinalis, Scutellaria baicalensis and Rosemary are mixed in a mass ratio of 1.5:1:0.3, crushed and passed through a 90-mesh sieve, added with 2.5 times the total mass of water, heated to 70° C., kept warm for 45 minutes, cooled to 8° C., soaked for 4 hours, added with ethanol so that the concentration of ethanol in the mixture is 70%, and precipitated at 18° C. for 4.5 hours to obtain an extract and a residue;

[0050] Step (2) adding water 13 times the mass of the residue and a composite bacterial strain 0.2 times the mass of the residue to the residue for fermentation at a fermentation temperature of 33° C. and a fermentation time of 12 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1.5×10 6 CFU / mg of Bacillus subtilis, the concentration is 1×10 6 CFU / mg of Candida albicans, the concentration was 1.5×10 6 CFU / mg of cellulose decomposing bacteria;

[0051] Step (3) After mixing the extract and the fermentation liquid, the mixture is concentrated at a concentration ratio of 1:2 to obtain a plant extract;

[0052] Yellow water is pretreated by the following methods:

[0053] Take the yellow water, filter and remove impurities, adjust the pH to 5.3 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 30%, with a concentration ratio of 1:3.

[0054] A method for preparing vinasse yellow water feed comprises the following steps:

[0055] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0056] Example 3

[0057] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 50 parts of distiller's grains, 10 parts of yellow water, 8 parts of plant extract, 2 parts of β-cyclodextrin, and 200 parts of basic feed;

[0058] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:5:7:3;

[0059] The method for preparing the plant extract comprises the following steps:

[0060] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary in a mass ratio of 1.7:1:0.2, crushing and passing through a 100-mesh sieve, adding water 3 times the total mass, heating to 75° C., keeping warm for 60 minutes, cooling to 10° C., soaking for 3-5 hours, adding ethanol so that the concentration of ethanol in the mixture is 75%, and precipitating at 20° C. for 6 hours to obtain an extract and a residue;

[0061] Step (2) adding water 15 times the mass of the residue and a composite bacterial strain 0.3 times the mass of the residue to the residue for fermentation at a fermentation temperature of 35° C. and a fermentation time of 13 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1.8×10 6CFU / mg of Bacillus subtilis, the concentration is 1.2×10 6 CFU / mg of Candida albicans, the concentration is 2×10 6 CFU / mg of cellulose decomposing bacteria;

[0062] Step (3) After mixing the extract and the fermentation liquid, the mixture is concentrated at a concentration ratio of 1:3 to obtain a plant extract;

[0063] Yellow water is pretreated by the following methods:

[0064] Take the yellow water, filter and remove impurities, adjust the pH to 5.5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 40%, with a concentration ratio of 1:5;

[0065] A method for preparing vinasse yellow water feed comprises the following steps:

[0066] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0067] In order to verify the properties of the distiller's grains yellow water feed provided in this application, the applicant set up comparative examples 1-7.

[0068] Comparative Example 1

[0069] Comparative Example 1 is the same as Example 1, except that the same mass of rosemary is used to replace scutellaria baicalensis, as follows:

[0070] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0071] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0072] The method for preparing the plant extract comprises the following steps:

[0073] Step (1) mixing magnolia bark and rosemary in a mass ratio of 1.4:1.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract and a residue;

[0074] Step (2) adding water 10 times the mass of the residue and a composite bacterial strain 0.1 times the mass of the residue to the residue for fermentation at a fermentation temperature of 30° C. for a fermentation time of 10 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1×10 6CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6 CFU / mg of cellulose decomposing bacteria;

[0075] Step (3) after mixing the extract and the fermentation liquid, concentrating the mixture at a concentration ratio of 1:1 to obtain a plant extract;

[0076] Yellow water is pretreated by the following methods:

[0077] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0078] A method for preparing vinasse yellow water feed comprises the following steps:

[0079] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0080] Comparative Example 2

[0081] Comparative Example 2 is the same as Example 1, except that the same mass of Magnolia officinalis is used to replace Scutellaria baicalensis, as follows:

[0082] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0083] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0084] The method for preparing the plant extract comprises the following steps:

[0085] Step (1) mixing magnolia bark and rosemary in a mass ratio of 2.4:0.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract and a residue;

[0086] Step (2) adding water 10 times the mass of the residue and a composite bacterial strain 0.1 times the mass of the residue to the residue for fermentation at a fermentation temperature of 30° C. for a fermentation time of 10 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1×10 6 CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6CFU / mg of cellulose decomposing bacteria;

[0087] Step (3) after mixing the extract and the fermentation liquid, concentrating the mixture at a concentration ratio of 1:1 to obtain a plant extract;

[0088] Yellow water is pretreated by the following methods:

[0089] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0090] A method for preparing vinasse yellow water feed comprises the following steps:

[0091] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0092] Comparative Example 3

[0093] Comparative Example 3 is the same as Example 1, except that the preparation method of the plant extract is different, as follows:

[0094] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0095] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0096] The method for preparing the plant extract comprises the following steps:

[0097] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary in a mass ratio of 1.4:1:0.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract;

[0098] Step (2) concentrating the extract at a concentration ratio of 1:1 to obtain a plant extract;

[0099] Yellow water is pretreated by the following methods:

[0100] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0101] A method for preparing vinasse yellow water feed comprises the following steps:

[0102] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0103] Comparative Example 4

[0104] Comparative Example 4 is the same as Example 1, except that the preparation method of the plant extract is different, as follows:

[0105] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0106] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0107] The method for preparing the plant extract comprises the following steps:

[0108] Step (1) Magnolia officinalis, Scutellaria baicalensis and rosemary are mixed in a mass ratio of 1.4:1:0.2, crushed and passed through an 80-mesh sieve, and 10 times the total mass of water and 0.1 times the total mass of the composite bacteria are added for fermentation at a fermentation temperature of 30°C and a fermentation time of 10 hours, sterilized, filtered, and a fermentation liquid is obtained; and the composite bacteria include a concentration of 1×10 6 CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6 CFU / mg of cellulose decomposing bacteria;

[0109] Step (2) After mixing the fermented liquids, the mixture is concentrated at a concentration ratio of 1:1 to obtain a plant extract;

[0110] Yellow water is pretreated by the following methods:

[0111] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0112] A method for preparing vinasse yellow water feed comprises the following steps:

[0113] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0114] Comparative Example 5

[0115] Comparative Example 5 is the same as Example 1, except that the yellow water is not pretreated, as follows:

[0116] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 4 parts of plant extracts, 1 part of β-cyclodextrin, and 100 parts of basic feed;

[0117] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0118] The method for preparing the plant extract comprises the following steps:

[0119] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary in a mass ratio of 1.4:1:0.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract and a residue;

[0120] Step (2) adding water 10 times the mass of the residue and a composite bacterial strain 0.1 times the mass of the residue to the residue for fermentation at a fermentation temperature of 30° C. for a fermentation time of 10 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1×10 6 CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6 CFU / mg of cellulose decomposing bacteria;

[0121] Step (3) after mixing the extract and the fermentation liquid, concentrating the mixture at a concentration ratio of 1:1 to obtain a plant extract;

[0122] A method for preparing vinasse yellow water feed comprises the following steps:

[0123] According to the formula, add distiller's grains, yellow water, plant extract and β-cyclodextrin into the basic feed and stir evenly.

[0124] Comparative Example 6

[0125] Comparative Example 6 is the same as Example 1, except that an equal mass of plant extract is used to replace β-cyclodextrin, as follows:

[0126] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 5 parts of plant extracts, and 100 parts of basic feed;

[0127] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0128] The method for preparing the plant extract comprises the following steps:

[0129] Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary in a mass ratio of 1.4:1:0.2, crushing and passing through an 80-mesh sieve, adding water twice the total mass, heating to 60° C., keeping warm for 30 minutes, cooling to 5° C., soaking for 3 hours, adding ethanol so that the concentration of ethanol in the mixture is 60%, and precipitating at 14° C. for 3 hours to obtain an extract and a residue;

[0130] Step (2) adding water 10 times the mass of the residue and a composite bacterial strain 0.1 times the mass of the residue to the residue for fermentation at a fermentation temperature of 30° C. for a fermentation time of 10 h, sterilizing, filtering, and obtaining a fermentation liquid; and the composite bacterial strain includes a concentration of 1×10 6 CFU / mg of Bacillus subtilis, the concentration is 0.5×10 6 CFU / mg of Candida albicans, the concentration is 1×10 6 CFU / mg of cellulose decomposing bacteria;

[0131] Step (3) after mixing the extract and the fermentation liquid, concentrating the mixture at a concentration ratio of 1:1 to obtain a plant extract;

[0132] Yellow water is pretreated by the following methods:

[0133] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0134] A method for preparing vinasse yellow water feed comprises the following steps:

[0135] According to the formula, add distiller's grains, yellow water and plant extract into the basic feed and stir evenly.

[0136] Comparative Example 7

[0137] Comparative Example 7 is the same as Example 1, except that the plant extract is replaced by an equal mass of β-cyclodextrin, as follows:

[0138] A distiller's grains and yellow water feed, comprising the following raw materials in parts by weight: 30 parts of distiller's grains, 5 parts of yellow water, 5 parts of beta-cyclodextrin, and 100 parts of basic feed;

[0139] The basic feed is a mixture of bran, bagasse, pineapple residue and tofu residue in a mass ratio of 10:2:1:9;

[0140] Yellow water is pretreated by the following methods:

[0141] Take the yellow water, filter and remove impurities, adjust the pH to 5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20%, with a concentration ratio of 1:2;

[0142] A method for preparing vinasse yellow water feed comprises the following steps:

[0143] According to the formula, add distiller's grains, yellow water and β-cyclodextrin into the basic feed and stir evenly.

[0144] The vinasse yellow water feed prepared in Examples 1-3 and Comparative Examples 1-7 were respectively used in the breeding of black soldier flies according to the following methods to obtain Application Examples 1-3 and Application Comparative Examples 1-7, which are specifically as follows:

[0145] S1, wheat bran, chicken feed and water were mixed and stirred at a mass ratio of 20:5:100, black soldier fly egg masses were placed, and cultured at a temperature of 26° C. and a humidity of 70% for 5 days to obtain primary culture larvae;

[0146] S2. The initially cultured larvae are placed in a lees yellow water feed at a feed-worm mass ratio of 2:1, and cultured at a temperature of 26° C. and a humidity of 70% for 8 days to obtain black soldier fly larvae.

[0147] The effects of the vinasse yellow water feed prepared in Examples 1-3 and Comparative Examples 1-7 on the growth of black soldier fly larvae were tested. The test results are shown in Table 1:

[0148] (1) Black soldier fly larvae survival rate and biomass obtained

[0149] Black soldier fly larvae survival rate = number of surviving larvae at the end / number of black soldier fly larvae put in × 100%; Black soldier fly larvae biomass = final total fresh weight of black soldier fly larvae - initial total fresh weight;

[0150] (2) Nutritional composition of black soldier fly larvae

[0151] The nutritional components of black soldier fly larvae were tested with reference to the AOAC official method for analysis of drugs in feed (2000): crude protein was determined by the Kjeldahl method, and crude fat was determined by the Soxhlet extraction method.

[0152] Table 1:

[0153]

[0154]

[0155] It can be seen from the data shown in Table 1 above that the application of the distiller's grains yellow water feed obtained in Examples 1-3 in the breeding of black soldier flies is more conducive to the survival of black soldier flies than the distiller's grains yellow water feed obtained in Comparative Examples 1-7, significantly improves the survival rate of black soldier flies, promotes the growth of black soldier flies, and obtains more crude protein.

[0156] By comparing Application Example 1 with Application Comparative Examples 1 and 2, it was found that using Magnolia officinalis, Scutellaria baicalensis and Rosemary as the raw materials of the plant extract, the synergistic effect of the three helps to improve the survival rate of black soldier flies; further comparing Application Example 1 with Application Comparative Examples 3 and 4, it was found that the preparation process of the plant extract, first alcohol extraction and then fermentation, the obtained plant extract contains rich active ingredients, which is more conducive to the growth and development of black soldier flies.

[0157] Comparison between Application Example 1 and Comparative Application Example 5 shows that the pretreated yellow water can provide more nutrients, is more suitable for the growth of black soldier flies, and increases the yield of black soldier flies.

[0158] Comparing Application Example 1 with Application Comparative Examples 6 and 7, it is shown that the plant extracts and β-cyclodextrin in the vinasse yellow water feed assist each other in enhancing the stress resistance of black soldier flies, improving their ability to adapt to environmental changes and resist diseases, and thereby improving the survival rate and quality of black soldier flies.

[0159] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A vinasse yellow water feed, characterized in that: The invention comprises the following raw materials in parts by weight: 30-50 parts of distiller's grains, 5-10 parts of yellow water, 4-8 parts of plant extracts, 1-2 parts of beta-cyclodextrin and 100-200 parts of basic feed.

2. The vinasse yellow water feed according to claim 1, characterized in that: The basic feed comprises bran, bagasse, pineapple residue and bean curd residue in a mass ratio of 10:(2-5):(1-7):(3-9).

3. The vinasse yellow water feed according to claim 1, characterized in that: The preparation method of the plant extract comprises the following steps: Step (1) mixing Magnolia officinalis, Scutellaria baicalensis and Rosemary, crushing and passing through an 80-100 mesh sieve, adding 2-3 times of the total mass of water, heating to 60-75° C., keeping warm for 30-60 minutes, cooling to 5-10° C., soaking for 3-5 hours, adding ethanol to make the concentration of ethanol in the mixture 60-75%, and precipitating at 14-20° C. for 3-6 hours to obtain an extract and a residue; Step (2) adding 10-15 times the mass of the residue of water and 0.1-0.3 times the mass of the residue of the composite bacteria to the residue for fermentation at a temperature of 30-35° C. for 10-13 h, sterilizing, filtering, and obtaining a fermentation liquid; Step (3) The extract is mixed with the fermentation liquid and concentrated to obtain a plant extract.

4. The vinasse yellow water feed according to claim 3, characterized in that: The mass ratio of Magnolia officinalis, Scutellaria baicalensis and Rosemary is (1.4-1.7):1:(0.2-0.5).

5. The vinasse yellow water feed according to claim 3, characterized in that: The composite bacteria include Bacillus subtilis, Candida albicans and cellulose decomposing bacteria.

6. The vinasse yellow water feed according to claim 5, characterized in that: The concentration of the Bacillus subtilis is (1-1.8)×10 6 CFU / mg, the concentration of the Candida albicans is (0.5-1.2)×10 6 CFU / mg, the concentration of the cellulose decomposing bacteria is (1-2)×10 6 CFU / mg.

7. The vinasse yellow water feed according to claim 3, characterized in that: The concentration ratio is 1:(1-3).

8. The vinasse yellow water feed according to claim 1, characterized in that: The yellow water is pretreated by the following method: Take the yellow water, filter and remove impurities, adjust the pH to 5-5.5 with feed-grade sodium hydroxide, and then pass it through the distillation system to increase the solid content of the yellow water to 20-40%, with a concentration ratio of 1:(2-5).

9. An application of the vinasse yellow water feed according to any one of claims 1 to 8 in black soldier fly breeding, characterized in that: The following steps are involved: S1, wheat bran, chicken feed and water are mixed and stirred in a mass ratio of 20:(5-15):100, and black soldier fly egg masses are placed for cultivation to obtain primary culture larvae; S2. Placing the initially cultured larvae in the yellow water feed of distiller's grains for cultivation to obtain black soldier fly larvae.

10. The use of the vinasse yellow water feed in black soldier fly breeding according to claim 9, characterized in that: The culture conditions in S1 are: temperature of 26-30° C., humidity of 60-80%, and time of 4-6 days; the culture conditions in S2 are: temperature of 26-30° C., humidity of 60-80%, and time of 7-10 days.

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