Composition and application thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a composition and application thereof. The invention relates to a barrier repair composition, in particular to a natural-source multi-effect barrier repair composition and application thereof. The invention provides vegetable oils, sphingolipids, sterols, isopentenol lipids, and polyphenols; wherein the vegetable oil comprises rose hip oil, pomegranate seed oil and borage oil. The barrier repairing composition can repair skin barriers, heal scratches and relieve skin allergy through the synergistic effect of all the components, and the components are natural, so that the barrier repairing composition is safe, stable and free of irritation.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and specifically, relates to a composition and an application thereof; more specifically, relates to a barrier repair composition with multiple functions of natural origin and an application thereof. Background Art

[0002] The skin barrier is the outermost layer of the skin, mainly composed of the stratum corneum, which plays an important role in protecting the human body. It is composed of keratinocytes, intercellular lipids (including ceramides, cholesterol and fatty acids) and a microbial layer, forming a complex and dynamic structure. The skin barrier plays a key role in moisturizing, protection, immune defense, sensation, metabolism and other aspects. Currently, skin diseases are closely related to impaired skin barrier function, such as psoriasis, atopic dermatitis and acne. Exposure to ultraviolet and visible light, air pollution, climate factors, chemical stimulation and aging can all affect the skin barrier function. Therefore, a healthy skin barrier is essential to maintaining the physiological state of skin health.

[0003] There are many reasons for skin barrier damage, such as excessive cleansing, exfoliation, use of inappropriate skin care products, exposure to ultraviolet rays or polluted environments, etc. There are many barrier repair ingredients on the market, such as natural lipids, moisturizers, anti-inflammatory ingredients, antioxidants, oil-containing ingredients, other active ingredients, etc. These ingredients with the effect of repairing the skin barrier are widely used in various skin care products.

[0004] However, current barrier repair products still have the following problems: 1. Formula stability issues of composite or single component. 2. Composite or single component, poor component permeability. The active ingredients of existing technologies or products are difficult to effectively penetrate deep into the skin, affecting the repair effect. 3. Skin irritation issues. Existing products are more or less irritating to the skin. 4. Because the barrier repair composition contains specific functional ingredients, such as unsaturated fatty acids, the product is prone to oxidation, deterioration or rancidity. Adding them to the product will directly affect the product quality. 5. In addition to various functional ingredients that have a repairing effect, existing technologies or commercially available cosmetics for repairing the skin barrier also add a variety of chemically synthesized raw materials, resulting in a poor overall feel.

[0005] Therefore, there is a need in the art for a barrier repair composition that has good stability, high safety, and is capable of repairing the skin barrier. Summary of the invention

[0006] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition that can solve the problems of safety, stability and irritation of existing products.

[0007] In view of this, in the first aspect of the present invention, the present invention provides a composition comprising

[0008] Plant oils, sphingolipids, sterols, prenol lipids, and polyphenols;

[0009] Among them, plant oils include rosehip oil, pomegranate seed oil, and borage oil.

[0010] The applicant creatively discovered that the composition of the present invention can repair the skin barrier, heal scratches, and relieve skin allergies through the synergistic effect of various ingredients, and the ingredients are derived from nature and are therefore safe, stable, and non-irritating.

[0011] Furthermore, the sphingolipid may be one or more of ceramide EOS, ceramide NS, ceramide NP, and ceramide AP; preferably, the sphingolipid is ceramide NS or ceramide NP.

[0012] Furthermore, the sterol may be one or more of cholesterol, phytosterol, lanosterol, and cholesterol; preferably, the sterol is phytosterol.

[0013] Furthermore, the isopentanol lipid may be one or more of vitamin E, coenzyme Q10, vitamin C, and squalene; preferably, the isopentanol lipid is squalene, and optionally, vitamin E is also included.

[0014] Furthermore, the polyphenol may be one or more of resveratrol, curcumin, catechin, anthocyanin, quercetin, isoflavone, and polyphenol epoxide; preferably, the polyphenol is resveratrol, curcumin, or quercetin.

[0015] Furthermore, the composition also includes a base oil.

[0016] Furthermore, the base oil is one or more of caprylic / capric triglyceride, triethylhexanoin, isononyl isononanoate, and dicaprylyl carbonate. In a specific embodiment, the base oil is caprylic / capric triglyceride.

[0017] Further, the vegetable oil accounts for 25% to 55% of the total mass of the composition, preferably 30% to 50%. For example, it can account for 30%, 35%, 40%, 45%, 50% of the total mass of the composition. For example, every 100g of the barrier repair composition of the present invention can contain 25g to 55g of vegetable oil, preferably 30g to 50g of vegetable oil.

[0018] Furthermore, the mass ratio of rosehip oil: pomegranate seed oil: borage oil in the vegetable oil is (1-5): (1-4): (1-3). For example, the mass ratio is 4:3:2, 3:3:2, 1:1:1, 5:3:2, 2:3:2. For example, every 100g of the barrier repair composition of the present invention may contain 15g of rosehip oil, 15g of pomegranate seed oil, and 15g of borage oil.

[0019] Furthermore, the sphingolipid accounts for 1% to 3% by weight of the entire composition, preferably 2%. For example, every 100g of the barrier repair composition of the present invention may contain 1g to 3g of sphingolipid, preferably 2g of sphingolipid.

[0020] Further, the sterol accounts for 2% to 8% by weight of the entire composition, preferably 5% to 8%. For example, it can account for 2%, 3%, 4%, 6%, or 7% by weight of the entire composition. For example, every 100 g of the barrier repair composition of the present invention can contain 2g to 8g of sterol, preferably 5g of sterol.

[0021] Further, the isopentanol lipid accounts for 30% to 50% of the mass of the whole composition, preferably 30% to 40%. For example, it can account for 30%, 35%, 40%, 45%, 50% of the mass of the whole composition. For example, every 100g of the barrier repair composition of the present invention can contain 30g to 50g of isopentanol lipid, preferably 30g to 40g of isopentanol lipid. Specifically, it includes 35g of squalene and 0.5g of vitamin E.

[0022] Furthermore, the polyphenol accounts for 0.5% to 3% of the mass of the entire composition, preferably 1%. For example, every 100g of the barrier repair composition of the present invention may contain 0.5g to 3g of polyphenol, preferably 1g of polyphenol.

[0023] Furthermore, the base oil accounts for 1-60% by weight of the entire composition, preferably 5-20%. For example, every 100 g of the barrier repair composition of the present invention may contain 1 g-60 g of base oil, preferably 5-20 g of base oil.

[0024] It should be noted that in the composition of the present invention, the content of each component can be adjusted arbitrarily according to the range to meet the needs of the art. For example, specifically, the upper limit of a certain component + the lower limit of other components can be ≤100, etc.

[0025] By configuring and using the components of the composition of the present invention according to the above ratios, the repairing effect of the barrier repairing composition can be further increased.

[0026] In a second aspect, the present invention provides a use of the above composition in repairing cosmetics and / or skin care products.

[0027] Furthermore, the present invention provides use of the composition of the present invention in repairing a barrier.

[0028] Furthermore, the cosmetics and / or skin care products can be used on the face or lips.

[0029] In a third aspect, the present invention provides a skin care product or cosmetic comprising the composition as described above.

[0030] Furthermore, the cosmetics may be makeup.

[0031] Furthermore, the product can be used on the face or lips.

[0032] Furthermore, the mass fraction of the composition in the skin care product or cosmetics is 0.01-5%. For example, it can be 0.01%, 0.05%, 0.1%, 0.5%, 1%, 2%, 3%, 4%, or 5%. Preferably, the mass fraction of the composition in the skin care product or cosmetics is 1%.

[0033] For example, every 100 g of the barrier repair product can include 0.01 g to 5 g of the composition of the present invention, such as 0.01 g, 0.05 g, 0.1 g, 0.5 g, 1 g, 2 g, 3 g, 4 g, or 5 g of the composition of the present invention. Preferably, every 100 g of the barrier repair product can include 3 g of the composition of the present invention.

[0034] Specifically, every 100 g of the barrier repair product may include 0.6 g of rosehip oil, 0.45 g of pomegranate seed oil, 0.3 g of borage oil, 0.06 g of ceramide NP, 0.15 g of phytosterols, 1.05 g of squalene, 0.015 g of vitamin E, and 0.03 g of resveratrol.

[0035] Furthermore, the barrier repairing product may be a skin care product or a cosmetic.

[0036] Furthermore, the cosmetics may be color cosmetics, such as air cushion, foundation, loose powder, pressed powder, primer, isolation, lipstick, blush, contouring, eye shadow, etc.

[0037] Furthermore, the product can be used on the face or lips.

[0038] The products used on the face can be eye shadow, makeup remover, cleanser, essence, toner, lotion, cream, etc.

[0039] The product used on the lips can be lipstick, lip glaze, lipstick, lip gloss, lip gloss, lip cream, etc.

[0040] The dosage form that can be used as a cosmetic is a stick, dish, ointment, or the like.

[0041] In the present invention, in addition to the above-mentioned ingredients, the product may also contain any ingredients commonly used in cosmetics and pharmaceuticals. As the optional ingredients, the following ingredients may be mentioned, and one or more than two may be contained as long as the effect of the present invention is exerted.

[0042] In some specific embodiments, the skin care products or cosmetics of the present invention further include one or more of emollients, fillers, colorants, synthetic waxes, oils, moisturizers, preservatives, adhesives, thickeners, active agents, or emulsifiers.

[0043] The skin care product or cosmetic of the present invention may contain any amount of water. As water, water used in cosmetics, quasi-drugs, etc. can be used, for example, ion exchange water, distilled water, ultrapure water, tap water, etc. can be used.

[0044] In some specific embodiments, the filler of the present invention can be one or more of silica, polymethylsilsesquioxane, mica, muscovite, phlogopite, synthetic mica, red mica, or biotite.

[0045] Furthermore, the product used on the lips may be a colorless lip product or a colored lip product.

[0046] Further, the lip product includes a colorant.

[0047] In some specific embodiments, the colored lip product contains a colorant, such as CI 77491, CI15850, CI 77499, CI 77891, CI 77492, etc., and may also be titanium dioxide, iron red, red 7, iron yellow, iron black, etc. Preferably, it may be one or more of the aforementioned colorants.

[0048] In some specific embodiments, the synthetic wax of the present invention can be exemplified by microcrystalline wax, beeswax, candelilla wax, ozokerite, cotton wax, carnauba wax, bayberry wax, insect wax, spermaceti, montan wax, rice bran wax, lanolin, kapok wax, acetylated lanolin, lanolin oil, sugarcane wax, isopropyl lanolin, hexyl laurate, hydrogenated lanolin, jojoba wax, hard lanolin, shellac wax, etc.

[0049] In some specific embodiments, the moisturizing agent of the present invention can include polyethylene glycol, propylene glycol, glycerol, 1,3-butylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucin sulfate, cholesterol-12-hydroxystearate, sodium lactate, bile acid salts, pyrrolidone carboxylates, short-chain soluble collagen, diglycerol PO adduct, yarrow extract, fumitory extract, etc.

[0050] In some specific embodiments, the preservatives of the present invention can include parahydroxybenzoates such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, butyl parahydroxybenzoate, benzoic acid, salicylic acid, sorbic acid, para-chloromecresol, hexachlorophene, benzalkonium chloride, chlorhexidine, trichlorocarbanilide, photosensitizer, phenoxyethanol, etc.

[0051] In some specific embodiments, the thickener of the present invention can include gum arabic, carrageenan, carrageenan, tragacanth gum, carob bean gum, casein, dextrin, gelatin, sodium pectate, sodium alginate, methyl cellulose, ethyl cellulose, CMC, hydroxyethyl cellulose, hydroxypropyl cellulose, PVA, PVM, PVP, sodium polyacrylate, carboxyvinyl polymer, locust bean gum, guar gum, tamarind gum, dialkyl dimethyl ammonium cellulose sulfate, xanthan gum, magnesium aluminum silicate, bentonite, hectorite, propolis, silicic anhydride, etc.

[0052] In some specific embodiments, the emulsifier of the present invention can include nonionic surfactants, amphiphilic polymers, such as (acrylate / methacrylate stearyl alcohol polyether-20) copolymer, hydrophobically modified alkyl cellulose, hydrogenated lecithin, sorbitan laurate, sorbitan palmitate, sorbitan oleate, sorbitan stearate, sorbitan isostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan tristearate, sorbitan sesquiisostearate.

[0053] In a specific embodiment, the skin care product or cosmetic can be an air cushion or foundation, including the composition of the present invention, vitamin E, PEG / PPG-18 / 18 polydimethylsiloxane, cetyl PEG / PPG-10 / 1 polydimethylsiloxane, isononyl isononanoate, polydimethylsiloxane & acrylates / polytrimethylsiloxane methacrylate copolymer, trimethylsiloxysilicate, quaternium-18 bentonite, CI 77891, CI 77492, CI 77491, CI 77499, synthetic fluorphlogopite, silica, polymethylsilsesquioxane, disodium EDTA, magnesium sulfate, glycerin, butylene glycol, 1,2-pentanediol, caprylyl glycol, and 1,2-hexanediol.

[0054] In a specific embodiment, the skin care product or cosmetic is isolated, including the composition of the present invention, disodium EDTA, magnesium sulfate, glycerin, 1,3-propylene glycol, 1,2-hexanediol, cyclopentasiloxane, butylene glycol dicaprylate / dicaprate, PEG-10 polydimethylsiloxane, cetyl PEG / PPG-10 / 1 polydimethylsiloxane, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer, sorbitan caprylate, camellia (CAMELLIA JAPONICA) seed oil, vitamin E, disteardimonium hectorite, CI 77891, CI 77492, CI 77491, CI 77499, and fragrance.

[0055] In a specific embodiment, the skin care product or cosmetic is a lipstick, comprising the composition of the present invention, caprylic / capric triglyceride, butylene glycol dicaprylate / dicaprate, octyldodecanol stearyloxystearate, diisostearyl malate, hydrogenated castor oil dimer linoleate, tridecyl trimellitate, polyethylene, ozokerite, candelilla wax, CI 15850 (lake), CI 77491, and fragrance.

[0056] In a fourth aspect, the present invention provides a synthetic carrier, on a portion or all of the surface of which there is at least one layer of the barrier repair composition of the present invention or the barrier repair product of the present invention.

[0057] In a fifth aspect, the present invention provides a method for preparing a skin care product or a cosmetic, comprising the following steps:

[0058] Mix rosehip oil, pomegranate seed oil, and borage oil until smooth.

[0059] At room temperature, add sphingolipids and mix well;

[0060] Add sterol and continue stirring to form a homogeneous liquid;

[0061] At room temperature, add isopentanol lipids and polyphenols and stir until completely homogeneous; and,

[0062] Homogenization. BRIEF DESCRIPTION OF THE DRAWINGS

[0063] Figure 1 is the NO inhibition rate of the composition of the present invention. DETAILED DESCRIPTION

[0064] The present invention will be described in detail below in conjunction with specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments and examples are used to illustrate the present invention, rather than to limit the present invention.

[0065] The term "rosehip oil" refers to a kind of wild rose fruit selected from South America, which is refined, extracted and concentrated by special technology. It is a pure natural plant oil without any chemical ingredients or preservatives. It mainly contains various unsaturated fatty acids, vitamin C, fruit acid, linoleic acid and other ingredients.

[0066] The term "pomegranate seed oil" refers to a vegetable oil selected from the Balkan Peninsula, extracted from pomegranate seeds. It contains a large amount of rich nutrients such as pomegranate acid and linoleic acid.

[0067] The term "borage oil" is a selected vegetable oil from Canada. Borage oil is rich in gamma-linolenic acid and a variety of nutrients.

[0068] The term "ceramide NP" is also known as sphingolipids, which are composed of sphingosine long-chain bases and fatty acids. It is a lipid present in the skin and plays a very important role in the formation of the surface stratum corneum.

[0069] The term "phytosterol" refers to a natural active ingredient in plants, which is similar in structure to animal sterols. Phytosterols have important physiological activities on the human body and have high permeability to the skin.

[0070] The term "squalene" is a highly stable and easily absorbed emollient. Squalane is usually derived from plants such as olive oil and sugar cane and is a common ingredient in skin care and hair care products.

[0071] The term "resveratrol", also known as resveratrol, is a phytoalexin produced by plants when they suffer from "adversity". It is a non-flavonoid polyphenol compound and is widely present in nature.

[0072] The term "curcumin" refers to a natural active substance extracted from turmeric (Curcuma longa L., belonging to the ginger family), which is a fat-soluble polyphenol compound.

[0073] The term "quercetin" is a polyhydroxyflavonoid compound that is widely found in the flowers, leaves, and fruits of plants and has multiple biological activities and high medicinal value.

[0074] Example 1: Formula for preparing cosmetics using the composition of the present invention

[0075] The formulas of the compositions of the present invention for preparing cosmetics are shown in Tables 1 and 2. Table 1 lists the components of various compositions for preparing cosmetics used in the present invention, and Table 2 lists the components of the comparative examples used in the present invention. The values ​​in the tables are all in mass percentage (wt%).

[0076] Table 1. Formula for preparing cosmetics based on the composition of the present invention

[0077]

[0078] Table 2: Formula for preparing cosmetics from comparative examples of the present invention

[0079]

[0080] Example 2: Preparation of cosmetics using the composition of the present invention

[0081] Mix the vegetable oil and base oil in proportion and stir well to ensure that the oils and other ingredients are fully blended.

[0082] Add sphingolipids: Add sphingolipids at room temperature and continue stirring until completely dissolved to keep the mixture homogeneous.

[0083] Add sterols: Slowly add the sterol raw material to the mixture and continue stirring to ensure that all ingredients are fully blended to form a homogeneous liquid.

[0084] Adding prenol lipids and polyphenols: at room temperature, add prenol lipid components and polyphenol components, and stir until completely uniform.

[0085] After homogenization, the stability and uniformity of the composition are ensured.

[0086] Packaging and storage: The prepared composition is injected into a sterile container, sealed and stored away from light and high temperature to ensure the activity and stability of the ingredients.

[0087] Example 3: Skin cell proliferation test of the composition of the present invention

[0088] This test is an in vitro efficacy evaluation test report for cell proliferation. It tests the effect of the test sample on keratinocyte viability after 24 hours of exposure to the test sample to evaluate the effect of the test sample on cell proliferation.

[0089] Materials and methods

[0090] 1. Cells

[0091] HaCaT keratinocytes.

[0092] 2. Main reagents

[0093] DMEM culture medium (Gibco), fetal bovine serum (Gibco), CCK8 kit.

[0094] 3. Main equipment

[0095] CO2 incubator (healforce), biological safety cabinet (healforce), multifunctional microplate reader (Tecan).

[0096] 4. Experimental methods

[0097] Plating: Cells were seeded in 96-well plates (1.5×10 4 / well) and incubated overnight in a 37°C, 5% CO2 incubator.

[0098] Administration: The blank control group (BC) was added with serum-free medium, the model group was added with serum-free medium, and the test sample group was added with drug-containing serum-free medium, and the culture was continued for 24 hours.

[0099] Detection: After 24 hours of culture, take out the 96-well plate, place it for about 10 minutes, equilibrate to room temperature, add CCK8 detection reagent, 10 μL / well, incubate at 37°C for 1 hour, read the OD value at 450nm (minus the OD value at 600nm); Cell viability calculation formula:

[0100] Cell viability (%) = OD value of sample group / OD value of blank control group × 100%

[0101] Statistical analysis

[0102] The mean value (Mean) and standard deviation (SD) of each group of experiments were calculated, and Graphpad Prism software was used for data statistical analysis. P < 0.05 was a significant difference, P < 0.01 was an extremely significant difference, and P > 0.05 was not significantly different. The specific results are shown in Table 3 (the detection concentration is 0.13% of the sample concentration of the composition of the present invention, and the solvent is ethanol). It can be seen from the table that the compositions of the present invention have the effect of promoting the proliferation of HaCaT cells, while the comparative example not only has no proliferation effect, but also has the effect of weakening the proliferation of HaCaT cells.

[0103] Table 3

[0104] name concentration(%) Cell viability (%) SD(%) P-value Blank control group BC / 100 4.09 / Example 2 0.13 107.27 1.88 ＜0.05 Embodiment 11 0.13 103.43 1.72 ＜0.05 Example 12 0.13 101.21 1.62 ＜0.05 Comparative Example 1 0.13 95.01 1.52 ＞0.05 Comparative Example 2 0.13 92.68 1.61 ＞0.05 Comparative Example 3 0.13 99.27 1.71 ＜0.05 Comparative Example 4 0.13 97.35 1.83 ＞0.05 Comparative Example 5 0.13 93.83 2.10 ＞0.05 Comparative Example 6 0.13 98.01 1.52 ＞0.05 Comparative Example 7 0.13 97.68 1.61 ＞0.05 Comparative Example 8 0.13 68.27 1.71 ＜0.05 Comparative Example 9 0.13 31.35 0.64 ＞0.05 Comparative Example 10 0.13 21.83 0.42 ＞0.05 Comparative Example 11 0.13 25.49 0.53 ＞0.05

[0105] Example 4: Scratch repair test of the barrier repair composition of the present invention

[0106] This test is divided into two parts. The first part conducts cytotoxicity testing on HaCaT human immortalized keratinocytes to determine the dosage concentration of the sample on HaCaT human immortalized keratinocytes; the second part tests the sample's ability to heal scratches on the HaCaT immortalized keratinocyte scratch model to evaluate the sample's repair efficacy.

[0107] Experimental Principle

[0108] The epidermis is mainly composed of keratinocytes. The keratinocytes located in the basal layer migrate upward after mitosis and gradually differentiate and mature, forming the spinous layer, granular layer and the stratum corneum located in the outermost layer of the epidermis in turn. The stratum corneum composed of keratinocytes and intercellular matrix is ​​essential for maintaining the skin barrier function. When the skin barrier is damaged, keratinocytes will migrate to the damaged area and repair it through proliferation, differentiation and lipid synthesis. The HaCaT cell line is a spontaneously transformed benign keratinocyte cell line of human skin. Because it has many similar characteristics with normal epidermal cells, it is often used to replace normal human keratinocytes for in vitro efficacy evaluation tests. Using HaCaT cells that have been fused into a monolayer state, an artificial blank area called a scratch is created to simulate the process of cell damage and barrier damage. The HaCaT cells at the edge of the scratch will migrate to the damaged area to heal the scratch. Some substances that promote cell proliferation and division, such as fetal bovine serum, can promote the healing of HaCaT cell scratches. Therefore, this method uses HaCaT cells to construct a scratch model in vitro. After adding the sample, the effect of the sample on promoting HaCaT cell scratch healing is studied through the change in the scratch healing area to evaluate the repair efficacy of the raw material.

[0109] Materials and methods

[0110] 1. Cells

[0111] HaCaT human immortalized keratinocytes.

[0112] 2. Main reagents

[0113] DMEM culture medium (Gibco), fetal bovine serum FBS (Gibco).

[0114] 3. Main equipment

[0115] CO2 incubator (healforce), biological safety cabinet (healforce), fluorescence inverted microscope (OLYMPUS).

[0116] 4. Experimental methods

[0117] The sample cytotoxicity experiment and efficacy evaluation test were carried out according to the "Q / YGEMG-SOP-2005 HaCaT immortalized keratinocyte scratch test operating procedures".

[0118] Cell plating: dilute the cell suspension to a density of 5×10 5 Each well was inoculated into a 6-well plate, 2 mL / well, and then placed in a 37°C, 5% CO2 incubator for 24 h.

[0119] Scratching and sample addition: Take out the 6-well plate, discard the supernatant, draw a horizontal line on the back of each well with a marker pen at the bottom of the well plate, draw 5 horizontal lines on average, add 1mL PBS to each well, draw a vertical straight line on the bottom of the 6-well plate with a gun tip, and slowly wash with PBS 3 times to remove the detached cells. The blank control group (BC) was added with serum-free medium, the positive control group was added with medium containing 10% fetal bovine serum, and the sample group was prepared with serum-free medium to the corresponding concentration, 2mL / well, and placed in a 37°C, 5% CO2 incubator for 24h±2h after the addition of the drug.

[0120] Photography: At 0 and 24 h after administration, the scratches in the 6-well plate were photographed under a microscope, and the scratch area A was calculated. The scratches were divided into 6 areas by the horizontal lines on the back, and the visual field under each area was photographed. The scratch positions in the visual fields selected at different time points should be consistent.

[0121] The scratch healing rate is calculated as follows:

[0122] Scratch healing rate (%) = [A (0h) - A (24h)] / A (0h) × 100%

[0123] Scratch healing efficacy: According to the average value B of the scratch healing rate of each experimental group, the keratinocyte scratch healing efficacy of samples of different concentrations in each experimental group was calculated. The formula is as follows:

[0124] Scratch healing efficacy (%) = [B (sample group) - B (blank control group)] / B (blank control group) × 100% The mean (Mean) and standard deviation (Standard Deviation, SD) of each group of tests were calculated, and Graphpad Prism software was used for plotting and data statistical analysis. P < 0.05 is a significant difference, P < 0.01 is a very significant difference; P > 0.05 is not significantly different. The specific results are shown in Table 4. It can be seen from the table that the repair barrier composition of the present invention has the best scratch healing rate.

[0125] Table 4

[0126]

[0127]

[0128] Example 5: Test on the soothing effect of the composition of the present invention

[0129] This test is divided into two parts. The first part is based on RAW264.7 macrophages to carry out cytotoxicity detection and determine the dosage concentration of the sample on RAW264.7 macrophages. The second part uses bacterial lipopolysaccharide (LPS) to induce the release of nitric oxide (NO) from the mouse mononuclear macrophage leukemia cell line RAW264.7, and uses the Griess method to determine the NO concentration in the cell culture supernatant, calculates the inhibition rate of the test substance on the release of NO from mouse macrophages, and evaluates the soothing effect of the test substance.

[0130] Experimental Principle

[0131] Cosmetics with soothing effects can help improve skin irritation. The occurrence of skin irritation mainly involves the physiological process of skin barrier-neurovascular-immune inflammation. Skin irritation problems can be improved by soothing skin inflammatory reactions. Nitric oxide (NO), as an immunomodulatory factor, participates in the physiological process of skin inflammatory reactions. By inhibiting the release of excessive NO by activated immune cells, the immune response of the skin can be reduced, thereby improving the irritation of the skin.

[0132] Materials and methods

[0133] 1. Experimental cells

[0134] Mouse mononuclear macrophage leukemia cell line RAW264.7.

[0135] 2. Experimental method: Sample cytotoxicity experiment and efficacy evaluation test were conducted according to “Q / YGEMG-SOP-2002RAW264.7 Macrophage Soothing Efficacy Assay Operating Procedure”.

[0136] 2.1 Cytotoxicity assay

[0137] 1) Cell plating: dilute the cell suspension to a density of 2×10 5 The cells / mL were inoculated into a 96-well plate, 100 μL / well, and after inoculation, the plate was placed in a 37°C, 5% CO2 incubator for 24 h.

[0138] 2) Sample addition: Take out the 96-well plate, discard the supernatant, add complete culture medium to the normal control group, add complete culture medium containing samples of different concentrations to the sample group, 100 μL / well, and culture in a 37°C, 5% CO2 incubator for 24 hours.

[0139] 3) Cell viability detection: Take out the 96-well plate, place it for about 10 minutes to balance, add CCK8 detection reagent, 10 μL / well, incubate at 37°C for 1 hour, and then detect the OD value; calculate cell viability:

[0140] Cell viability (%) = sample OD value / blank OD value × 100%

[0141] Note: Subsequent efficacy evaluation tests were conducted on samples with cell viability above 90%, and the specific results are shown in Table 5. The barrier repair composition of the present invention showed no cytotoxicity within the concentration range of 0.13%-0.5%.

[0142] Table 5

[0143]

[0144] 2.2 Efficacy evaluation

[0145] 1) Cell plating: Same as above.

[0146] Note: Each test includes a blank control group, a model group, a test sample group and a positive control group, and three replicate wells are set up in each group.

[0147] 2) Sample addition: After culturing for 24 hours, carefully discard the culture supernatant and set up the following groups: ① Blank group: 100 μL complete culture medium; ② LPS model group: LPS with a final concentration of 1 μg / mL; ③ Experimental group: different concentrations of samples and LPS with a final concentration of 1 μg / mL; ④ Positive control group: dexamethasone and LPS with a final concentration of 1 μg / mL; incubate in an incubator for 24 hours.

[0148] Note: The blank control group only contained complete culture medium, and the positive control group contained complete culture medium containing positive substances.

[0149] 3) Collection of cell supernatant: After the incubation is completed, the supernatant is collected.

[0150] 4) Detection: Detect the NO content according to the kit instructions.

[0151] The mean value (Mean) and standard deviation (SD) of each group of experiments were calculated, and Graphpad Prism software was used for data statistical analysis. p < 0.05 was considered a significant difference, p < 0.01 was considered an extremely significant difference, and p > 0.05 was considered to be insignificant.

[0152] NO inhibition rate (%) = (model group - sample group) / model group × 100%; the specific results are as follows Figure 1 As shown, at a concentration of 0.50%, the skin barrier repair composition has an average NO inhibition rate of 49.11% (p<0.001), and Examples 1 to 4 all have very good NO inhibition rates.

[0153] Example 6: Test of the repair effect of the composition of the present invention

[0154] Experimental system

[0155] Cell line: human immortalized keratinocytes (HaCaT).

[0156] Cell generation number: at least 2 generations.

[0157] The number of cells per group: 6×10 5 Pieces / hole.

[0158] Principle and method

[0159] As we age, the skin is affected by the external environment (physical damage, chemical reagents, etc.), causing the skin to age. During the aging process, cell damage gradually accumulates, further accelerating skin aging. Filaggrin (FLG) is an important molecule that connects keratin fibers in the stratum corneum of human skin. With the help of FLG monomers, keratin fibers gather regularly and form a solid physical barrier in the outermost layer of the epidermis, which can prevent the loss of epidermal moisture and the invasion of external allergic substances. In this experiment, human immortalized keratinocytes HaCaT were used to establish an experimental model, and the relative expression of FLG genes and FLG protein expression were detected to evaluate whether the samples have repair effects.

[0160] Experimental procedures

[0161] Relative expression of FLG gene

[0162] 1. Inoculate cells in 6-well plates (6×10 5 / well) and incubated at 37°C, 5% CO2 for 24 h.

[0163] 2. After the incubation, the cells were gently rinsed once or twice with D-Hanks and fresh culture was added to the normal control group.

[0164] Fresh culture medium containing samples was added to the sample group and incubated at 37°C, 5% CO2 for 24 h.

[0165] 3. After the culture, the cells were sampled, the total RNA of each experimental group was extracted, cDNA was synthesized, and the gene expression of β-actin and the target gene was detected by q-PCR.

[0166] 4. Use β-actin as an internal reference for gene expression and calculate the relative RNA expression of the target gene.

[0167] Relative RNA expression △CT=CT 目的基因 -CT 内参基因

[0168] △△CT=△CT test -△CT control

[0169] RE=2- △△ CT

[0170] FLG protein expression detection

[0171] 1. Inoculate cells in 6-well plates (6×10 5 / well) and incubated at 37°C, 5% CO2 for 24 h.

[0172] 2. After the incubation, the cells were gently washed once or twice with D-Hanks, fresh culture medium was added to the normal control group, and fresh culture medium containing the sample was added to the sample group, and the cells were incubated at 37°C and 5% CO2 for 24 h.

[0173] 3. After the culture is completed, the cells are collected and lysed, and the detection is performed according to the operating instructions of the ELISA kit, and the upregulation rate is calculated by the following formula.

[0174]

[0175] F: FLG protein expression

[0176] Judgment basis

[0177] Statistical analysis showed that p<0.05 was significantly different. The specific results are shown in Table 6. The expression level of FLG protein and the relative expression level of FLG gene were significantly increased compared with the normal control group, indicating that the composition has a significant repair effect.

[0178] Table 6

[0179]

[0180] Example 7: Preparation of the composition of the present invention and testing of barrier repair products

[0181] According to the formulas in Tables 7 to 10, air cushion, foundation, primer and lipstick were prepared respectively.

[0182] Table 7

[0183]

[0184]

[0185] Table 8

[0186]

[0187]

[0188] Table 9

[0189]

[0190]

[0191] Table 10

[0192]

[0193] Example 8 Preparation of the composition of the present invention and testing of barrier repair products

[0194] The barrier repair compositions of Examples 1-8 were respectively prepared into an air cushion according to the formula in Table 7 (corresponding to Product 1, Product 2, Product 3, Product 4, Product 5, Product 6, Product 7, Product 8) for efficacy evaluation.

[0195] 180 trial participants aged between 20 and 45 were selected and divided into three skin types according to their skin sensitivity from weak to strong. Among them, 45 of them occasionally experienced mild facial itching, flushing, dryness, etc. in daily skin care, which was type I skin; 72 of them often experienced facial itching, flushing, dryness, desquamation, etc. in daily skin care, which was type II skin; 63 of them had severe facial itching, flushing, dryness, pain, desquamation, etc., which was type III skin. The participants of each sensitive skin type were divided into 9 groups on average, with 20 people in each group trying products 1 to 8 for 2 consecutive weeks, once in the morning and once in the evening.

[0196] Visia was used to test the changes in the red areas of the test subjects' facial skin, the changes in the transepidermal water loss and the total moisture content of the skin, and to compare the skin repair effects.

[0197] Test conditions: (1) Indoor environment; (2) No strong sunlight or direct light; (3) Ambient temperature 22±2℃, ambient humidity 40-60%;

[0198] Test area: Whole face.

[0199] Test steps: First, measure the blank value (red zone area, transepidermal water loss, skin moisture content) of the tested area 30 minutes after cleaning, then require the trial participants to use the corresponding products every morning and evening, and after 2 weeks, test the red zone area, transepidermal water loss and skin moisture content in the area respectively.

[0200] Repair judgment criteria:

[0201] Ineffective: The skin red area is reduced by less than 20%, the transepidermal water loss is reduced by more than 10%, and the skin water content is increased by less than 10%;

[0202] Effective: The skin red area is reduced by more than 20%, the transepidermal water loss is reduced by more than 10%, and the skin water content is increased by less than 20%;

[0203] Significant effect: The skin red area was reduced by more than 50%, transepidermal water loss was reduced by more than 10%, and skin water content was increased by more than 30%;

[0204] Cured: the area of ​​skin red zone decreased by more than 80%; transepidermal water loss decreased by more than 20%, and skin water content increased by more than 40%; the specific results are shown in Table 11. It can be seen from the table that the barrier repair composition of the present invention applied to the air cushion product can obtain a good repair effect, which can not only repair the skin stratum corneum, enhance the skin barrier, and strengthen cell moisturizing repair, but also has the characteristics of good absorption, safe ingredients and multiple protection functions.

[0205] Table 11

[0206]

[0207]

Claims

1. A composition comprising Plant oils, sphingolipids, sterols, prenol lipids, and polyphenols; in, Plant oils include rosehip oil, pomegranate seed oil, and borage oil.

2. The composition according to claim 1, characterized in that The vegetable oil accounts for 25-55% of the mass of the whole composition; the sphingolipids account for 1-3% of the mass of the whole composition; the sterols account for 2-8% of the mass of the whole composition; the isopentanol lipids account for 1-3% of the mass of the whole composition; and the polyphenols account for 0.5-3% of the mass of the whole composition.

3. The composition according to claim 2, characterized in that The sphingolipid is one or more of ceramide EOS, ceramide NS, ceramide NP, and ceramide AP; preferably, the sphingolipid is ceramide NS, ceramide AP, or ceramide NP.

4. The composition according to claim 1, characterized in that The sterol is one or more of cholesterol, phytosterol, lanosterol and cholesterol; preferably, the sterol is phytosterol.

5. The composition according to claim 1, characterized in that The isopentanol lipid is one or more of vitamin E, coenzyme Q10, vitamin C, and squalene; preferably, the isopentanol lipid is squalene, and optionally, vitamin E is also included.

6. The composition according to claim 1, characterized in that The polyphenol is one or more of resveratrol, curcumin, catechin, anthocyanin, quercetin, isoflavone, and polyphenol epoxide; preferably, the polyphenol is resveratrol, curcumin, or quercetin.

7. The composition according to claim 1, characterized in that The mass ratio of rosehip oil:pomegranate seed oil:borage oil in the vegetable oil is (1-5):(1-4):(1-3).

8. Use of the composition according to any one of claims 1 to 7 in cosmetics and / or skin care products for repair.

9. A skin care product or cosmetic comprising the composition according to any one of claims 1 to 7.

10. A synthetic carrier, wherein at least one layer of the composition according to any one of claims 1 to 7 is present on part or all of the surface of the carrier.

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