Composition containing plant seed grease and application thereof

Abstract

The invention provides a composition containing plant seed grease and a preparation method thereof. The plant seed oil is selected from simmondsia chinensis seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil, macadamia nut seed oil, castor seed oil, peony seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, zucchini seed oil, linseed oil, rapeseed oil, sesame seed oil and the like or a combination thereof. Based on the total weight of the composition, the composition comprises at least 0.5 wt% of plant seed grease. The invention also relates to an application of the composition containing the plant seed grease in enhancing the skin barrier function and / or in preparing a medicine for enhancing the skin barrier function and / or an external preparation for the skin.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to a composition containing plant seed oil and its application in improving skin barrier function. Background Art

[0002] As the largest organ, the function of the skin is to protect us from environmental damage, prevent microbial invasion, regulate temperature, and maintain hydration. A defective skin barrier allows irritants, allergens, and microorganisms to invade, and coupled with an excessive immune response, leads to skin inflammation. Therefore, many manufacturers and consumers are relentlessly looking for the "ideal" moisturizer to repair the skin barrier. The common function of moisturizing ingredients is to keep the stratum corneum smooth and elastic by increasing the water content of the stratum corneum and enhancing the adhesion of keratinocytes. Moisturizers repair the skin barrier mainly through two mechanisms. One is to provide hydration through hydrophilic ingredients such as glycerol; the other is to seal the skin through hydrophobic ingredients such as oils to prevent TEWL. Some hydrophobic ingredients may diffuse into the intercellular matrix of the stratum corneum to enhance the integrity of the barrier structure.

[0003] According to the source and chemical composition of oils and fats, they can be mainly divided into: plant oils and fats, animal oils and fats, mineral oils and fats and synthetic oils. Plant oils and fats are favored by many skin care products because of their skin-friendly texture, close to the structure of human sebum membrane, good permeability, deep penetration and nourishment of the skin. Plant oils and fats used in cosmetics mainly come from plant fruits, seeds and germs, and some also come from other parts of plants such as leaves, skins, roots, petals and stamens. Oils containing plant seeds mainly include jojoba (Simmondsia chinensis) seed oil, safflower (Carthamus tinctorius) seed oil, white pond flower (Limnanthes alba) seed oil, sunflower (Helianthus annuus) seed oil, macadamia (Macadamiaternifolia) seed oil, castor (Ricinus communis) seed oil, peony (Paeonia suffruticosa) seed oil, grape (Vitis vinifera) seed oil, grapefruit (Citrus paradisi) seed oil, apple (Pyrus malus) seed oil, pomegranate (Punica granatum) seed oil, watermelon (Citrullus lanatus) seed oil, zucchini (Cucurbita pepo) seed oil, flax (Linum usitatissimum) seed oil, rapeseed (Brassica campestris) seed oil, sesame (Sesamumindicum) seed oil, Borago (Borago officinalis seed oil, Sclerocaryabirrea seed oil, Gossypium herbaceum seed oil, Camellia sinensis seed oil, Coixlacryma-jobi ma-yuen seed oil, Carica papaya seed oil, Rubus idaeus seed oil, Adansonia digitata seed oil, Calendula officinalis seed oil, Carapa guaianensis seed oil, Moringa oleifera seed oil, Caryaillinoinensis seed oil, Perilla ocymoides seed oil, Orbignya oleifera seed oil, and Lupinus albus seed oil.

[0004] Jojoba oil has a very significant cosmetic effect on the skin. It can unclog pores, regulate the oil secretion of oily or mixed skin, and improve inflamed skin, eczema, psoriasis, acne, etc. It can restore the vitality and luster of dry hair and dry wrinkled skin, so it is a base oil that can be used for all skin types. For example, the document Li Chunxia, ​​Application of Jojoba Oil in Cosmetics, Daily Chemicals Science [J], 2007, 30 (2) pointed out that jojoba oil can significantly reduce the loss of epidermal water by not completely blocking the evaporation of gas and water. In addition, as an excellent moisturizer, it has the effect of easy spreading and lubrication, making the skin smooth and elastic.

[0005] Safflower seed oil contains 6% saturated fatty acids, 21% oleic acid, and 73% linoleic acid. Since its main component is linoleic acid, which is the highest linoleic acid oil among edible oils, it has high nutritional value and has attracted much attention in medicine, food, and cosmetics. For example, the document Bao Leer, Research Progress of Safflower Seed Oil, World Latest Medicine Information (Electronic Version) [J], 2021, 21 (27): 146-148 points out that safflower seed oil has antioxidant effects, can effectively remove free radicals and reduce the ability of free radicals caused by chemical factors, and also has anti-aging effects. The document Jiang Li, Analysis of the Efficacy and Application Prospects of Safflower Seed Oil, Agricultural Products Processing [J], 2017, 6: 56-58 points out that long-term consumption of safflower seed oil can effectively prevent atherosclerosis, reduce blood lipids and serum cholesterol, soften blood vessels, and indirectly restore the nervous system and other pharmacological functions.

[0006] Limnanthes alba seed oil is one of the most stable vegetable oils known, and its high stability is due to the presence of natural antioxidant tocopherol. For example, the literature Su Dihan, Analysis of trace components in several high oxidative stability vegetable oils and their anti-ultraviolet antioxidant properties, Fragrances and Cosmetics [J], 2019, 4:47-51 detected the content and antioxidant properties of tocopherol in Limnanthes alba seed oil. By removing DPPH, it was shown that Limnanthes alba seed oil has certain antioxidant properties, can protect against ultraviolet rays, promote skin microcirculation, and make the face naturally ruddy and energetic.

[0007] Skin barrier generally refers to the physical barrier of the skin, including the stratum corneum of the epidermis and the sebum membrane located on the surface of the stratum corneum. The stratum corneum is located in the outermost layer of the epidermis and has a "brick-wall structure", in which "bricks" refer to keratinocytes and "mortar" refers to intercellular lipids. In the process of skin barrier formation, more than 200 genes are highly related to the skin barrier. For example, the barrier factors related to the formation of the keratin capsule are filaggrin (FLG), loricrin (LOR), intracellular leukocyte protein (IVL), transglutaminase 1 (TGM1), and the barrier moisturizing-related factors are caspase 14 (CASP14), HMGCoA reductase (HMGCR), aquaporin 3 (AQP3) and the intercellular junction-related factor of keratinocytes, zonula occludens 1 (ZO-1).

[0008] Specifically, filaggrin (FLG) is an important component protein of the cornified envelope of the outer layer of the epidermis. It is mainly present in the granular layer and the clear layer of the epidermis and is an important component structure of the skin barrier. It can be further degraded into hydrophilic free amino acids and water-absorbing derivatives, which help the skin store moisture, maintain the skin's moisture content, and have a moisturizing effect. Loricrin (LOR) is the main component of the cornified envelope and plays an important role in the barrier function of the epidermis. Indigoferin (IVL) is mainly expressed in the upper part of the spinous cell layer and the granular layer. It is a marker protein for keratinocyte differentiation and is located in the outer layer of the cornified envelope. It covalently binds to ceramides containing hydroxyl-OH, thereby connecting the lipid matrix and cornified cells to play a role. The characteristic resistance and insolubility of the cornified envelope are based on the formation of very stable isopeptide bonds catalyzed by transglutaminase 1 (TGM1).

[0009] HMGCoA reductase (HMGCR) is the rate-limiting enzyme for cholesterol synthesis. It exists in the endoplasmic reticulum and catalyzes the synthesis of methyldihydroxyvaleric acid. After decarboxylation and phosphorylation, squalene is generated. Finally, squalene is cyclized to lanosterol and then converted to cholesterol. Aquaporin 3 (AQP3) is the most abundant aquaporin subtype in the AQPs family. Its expression in the epidermis has a spatial hierarchy. It is mainly expressed in the basal layer, spinous cell layer, and granular layer of the human epidermis, and gradually disappears in the stratum corneum. This spatial distribution is consistent with the water content distribution of the skin: the water content of the basal layer and the upper part of the basal layer is about 75%, while the stratum corneum is only about 10%-15%. The high expression of AQP3 in the basement membrane zone can promote the transport of water, glycerol and urea, making the extracellular environment of the basal layer closer to a neutral equilibrium state; and the closer to the stratum corneum, the less AQP3 is expressed, the water content decreases significantly, and the internal environment of the skin gradually becomes acidic. In addition, AQP3 plays an important role in the transport of glycerol in the stratum corneum. AQP3 transports endogenous glycerol and triglycerides in the sebaceous glands into the epidermis, directly or indirectly affecting the skin's moisturizing effect.

[0010] Tight junctions are an important component of the physical barrier, mostly appearing in the epidermis of the skin, located on the apical side of the gaps between adjacent cells. Tight junctions are composed of different types of transmembrane proteins and intracellular cytoplasmic proteins. Transmembrane proteins include CLDN1 and OCLN, while cytoplasmic proteins include zonula occludens-1 (ZO-1). ZO-1 is a cytoplasmic protein belonging to the membrane-bound guanylate kinase protein family. It aggregates OCLN and CLDN1 through extracellular signal transduction pathways to form basic cell connection strips. ZO-1 is the most characteristic cytoplasmic protein in the skin barrier function.

[0011] Phyto Bionic Sebum (PBS technology) is a technology that selects a variety of natural plant oils and simulates the natural lipid components in the skin barrier structure through scientific proportions. It replenishes the unsaturated fatty acids necessary for the skin's stratum corneum, such as linolenic acid and linoleic acid, and replenishes the lipid components missing from the skin. It is compatible with sebum, thereby quickly repairing the skin barrier and restoring the skin to a healthy, moisturized and lustrous state.

[0012] Among them: vegetable oils are mainly derived from plant fruits (for example: avocado oil, olive oil, almond oil, avocado oil, sea buckthorn oil, coconut oil, orange oil, bergamot oil, citron oil, tomato oil, dog rose oil, pepper oil, Brazilian nectarine oil, flat-leaved vanilla oil, anise oil, maury palm oil, juniper fruit oil, mountain pepper oil, Wenzhou mandarin oil, star palm oil, wild rose oil and coriander oil, etc.), seeds (for example: jojoba seed oil, safflower seed oil, white pondweed seed oil, sunflower seed oil, macadamia seed oil, castor seed oil, peony Seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, zucchini seed oil, linseed oil, rapeseed oil, sesame seed oil, borage seed oil, walnut seed oil, cottonseed oil, tea seed oil, coix seed oil, papaya seed oil, raspberry seed oil, baobab seed oil, calendula seed oil, balsam pear seed oil, moringa seed oil, pecan seed oil, perilla seed oil, babassu seed oil and white lupine seed oil, etc.) and germ (for example: rice germ oil, wheat germ oil, oat germ oil and corn germ oil, etc.), and some are also derived from other parts of the plant such as leaves, skin, roots, petals and stamens.

[0013] The present invention unexpectedly found that plant seed oils (e.g., jojoba oil, safflower seed oil, sunflower seed oil, etc.) have skin barrier-related effects, can enhance skin barrier vitality and promote the expression of skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, this composition containing plant seed oil can be used as an efficacy additive in medicines and skin topical preparations. Summary of the invention

[0014] On the one hand, the present invention provides a composition comprising plant seed oil, wherein the plant seed oil is selected from: jojoba seed oil, safflower seed oil, white meadowfoam seed oil, sunflower seed oil, macadamia nut seed oil, castor seed oil, peony seed oil, grape seed oil, grapefruit seed oil, apple seed oil, pomegranate seed oil, watermelon seed oil, zucchini seed oil, linseed oil, rapeseed oil, sesame seed oil, borage seed oil, walnut seed oil, cottonseed oil, tea seed oil, coix seed oil, papaya seed oil, raspberry seed oil, baobab seed oil, calendula seed oil, balsam pear seed oil, moringa seed oil, pecan seed oil, perilla seed oil, babassu seed oil, white lupin seed oil or a combination thereof, and the composition comprises at least 0.5 weight% of the plant seed oil based on the total weight of the composition.

[0015] In a preferred embodiment, the composition comprising plant seed oils and fats further comprises a natural surfactant selected from the group consisting of lecithin, cholesterol, lanolin, tea saponin, protein, saponin, sugar, alkyl polyglycosides or a combination thereof. In a preferred embodiment, the natural surfactant is hydrogenated lecithin.

[0016] In a preferred embodiment, the weight ratio of the plant seed oil to the natural surfactant in the composition is 1: 1 to 20: 1. In a more preferred embodiment, the weight ratio of the plant seed oil to the natural surfactant in the composition is 10: 1 to 20: 1.

[0017] On the other hand, the present invention also relates to the use of a composition comprising plant seed oil in enhancing skin barrier function. In a preferred embodiment, the enhancement of skin barrier function is achieved by increasing skin barrier activity and / or promoting the expression of skin barrier-related factors.

[0018] In another aspect, the present invention also relates to the use of a composition comprising plant seed oils in the preparation of a medicine and / or a skin topical preparation for enhancing skin barrier function. In a preferred embodiment, the medicine and / or skin topical preparation comprises 0.001-100% by weight of the composition. DETAILED DESCRIPTION

[0019] The present invention relates to a composition comprising plant seed oil and its application. In the study, it was found that the composition has the effect of enhancing skin barrier vitality and promoting the expression of skin barrier related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1. Therefore, the composition comprising plant seed oil can be used as an efficacy additive for preparing medicines and / or skin external preparations to enhance skin barrier function.

[0020] In order to provide a more concise description, some quantitative expressions given herein are not modified by the term "about". It should be understood that, whether or not the term "about" is explicitly used, each quantity given herein is intended to refer to the actual given value, and is also intended to refer to the approximate values ​​of these given values ​​that can be reasonably inferred by ordinary technicians in the field, including the approximate values ​​of these given values ​​caused by experimental and / or measurement conditions.

[0021] To provide a more concise description, some quantitative expressions herein are described as a range of about X amount to about Y amount. It should be understood that when describing a range, the range is not limited to the upper and lower limits described, but should include the entire range of about X amount to about Y amount or any amount therebetween.

[0022] Plant seed oil

[0023] The plant seed oil used in the present invention is also called plant seed oil, including but not limited to: jojoba seed oil, safflower seed oil, meadowfoam seed oil and sunflower seed oil. Oils containing plant seeds mainly include jojoba (Simmondsiachinensis) seed oil, safflower (Carthamus tinctorius) seed oil, white pond flower (Limnanthes alba) seed oil, sunflower (Helianthus annuus) seed oil, macadamia (Macadamia ternifolia) seed oil, castor (Ricinuscommunis) seed oil, peony (Paeonia suffruticosa) seed oil, grape (Vitis vinifera) seed oil, grapefruit (Citrus paradisi) seed oil, apple (Pyrus malus) seed oil, pomegranate (Punica granatum) seed oil, watermelon (Citrullus lanatus) seed oil, zucchini (Cucurbita pepo) seed oil, flax (Linum usitatissimum) seed oil, rapeseed (Brassica campestris seed oil, sesame (Sesamumindicum) seed oil, borage (Borago officinalis) seed oil, walnut (Sclerocarya birrea) seed oil, cotton (Gossypium herbaceum) seed oil, tea (Camellia sinensis) seed oil, chuangu (Coix lacryma-jobi ma-yuen) seed oil, papaya (Carica papaya) seed oil, raspberry (Rubus idaeus) seed oil, baobab (Adansonia digitata) seed oil, calendula (Calendula officinalis) seed oil, bitter oil tree (Carapa guaianensis) seed oil, moringa (Moringa oleifera) seed oil, American pecan (Carya illinoinensis) seed oil, perilla (Perilla ocymoides) seed oil, babassu (Orbignya oleifera) seed oil and white lupin (Lupinus albus) seed oil, etc.

[0024] For example, jojoba seed oil is the most permeable base oil, which is easily absorbed by the skin. It is refreshing, moisturizing and non-greasy. It can restore the pH balance of the skin, remove wrinkles, effectively improve oily skin, regulate the secretion function of sebaceous glands, shrink pores, and is also the best skin moisturizing oil. Safflower seed oil is rich in linoleic acid and fat-associated substances (vitamin E, vitamin A). It is easy to deteriorate when in contact with air. Its nutritional ingredients and applications are similar to those of sunflower oil. It is usually used as a moisturizing and lubricating ingredient in cosmetics. It can improve eczema and rough skin, make the skin supple and smooth, and help normalize metabolism. White pond flower seed oil contains more than 98% long-chain fatty acids with antioxidant effects. It has a unique molecular structure with moisturizing function. It can form a water-locking film on the skin surface to lock in moisture and resist dryness. It can also stretch the skin texture, deeply repair skin loss, and enhance the overall contour of the skin. Macadamia seed oil is rich in unsaturated fatty acids, which can prevent heart disease. This natural plant oil with a very high content of monounsaturated fatty acids can regulate and control blood sugar levels and improve lipid metabolism in diabetic patients. It can also effectively moisturize the skin and is a good sunscreen and skin care product.

[0025] The present invention unexpectedly discovered that a composition comprising plant seed oil (e.g., jojoba seed oil, safflower seed oil, sunflower seed oil, etc.) has skin barrier-related efficacy, can enhance skin barrier vitality and promote the expression of skin barrier-related factors FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1.

[0026] In some embodiments, the composition of the present invention comprises 0.1-100% by weight of a plant seed oil. In a preferred embodiment, the composition of the present invention comprises 0.1-50% by weight of a plant seed oil. In a preferred embodiment, the composition of the present invention comprises 0.1-20% by weight of a plant seed oil. In a preferred embodiment, the composition of the present invention comprises 0.5-10% by weight of a plant seed oil.

[0027] Natural surfactants

[0028] The composition of the present invention may also contain natural surfactants. Natural surfactants are mostly derived from animals and plants and are relatively complex macromolecular organic substances. Due to their strong hydrophilicity, they can form emulsions. These substances often have a high viscosity, which is beneficial to the emulsion stability.

[0029] The natural surfactants contained in the composition of the present invention include, but are not limited to, lecithin, cholesterol, lanolin, tea saponin, protein, saponin, sugar, alkyl polyglycosides, etc. Lecithin exists in biological cells, such as animal eggs, brain and other tissues, and seeds or embryos of plants. Yolk phospholipids are extracted from egg yolks; soybeans are rich in lecithin. Lecithin has physiological activities such as emulsification, dispersion, and anti-oxidation, and is a natural excellent surfactant and an important emulsifier.

[0030] Hydrogenated lecithin has strong hydrophilicity and moisturizing properties, and has a strong affinity for the skin and mucous membranes. It can play a role in moisturizing, emulsifying and dispersing when used in cosmetic formulas. As a surfactant, it can also condition the skin to achieve a good oil-water balance. Hydrogenated lecithin can also be used to develop high-end cosmetic products such as skin care creams, hand creams, lipsticks, sunscreens, etc.

[0031] In some embodiments, the composition of the present invention comprises 0.1-20% by weight of a natural surfactant. In a preferred embodiment, the composition of the present invention comprises 0.1-10% by weight of a natural surfactant. In a preferred embodiment, the composition of the present invention comprises 0.1-5% by weight of a natural surfactant. In a preferred embodiment, the composition of the present invention comprises 0.5-2% by weight of a natural surfactant.

[0032] In a preferred embodiment, the weight ratio of plant seed oil to natural surfactant in the composition of the present invention is 1: 1 to 20: 1. In a more preferred embodiment, the weight ratio of plant seed oil to natural surfactant in the composition is 10: 1 to 20: 1.

[0033] Medicines and / or topical skin products

[0034] The composition comprising the plant seed oil can be applied to medicines and / or external skin preparations for enhancing the skin barrier function.

[0035] In some embodiments, the drug is selected from the group consisting of tablets, capsules, emulsions, suspensions, powders, granules, solutions, and various pharmaceutical dosage forms known in the art. Different dosages are added according to different types of dosage forms.

[0036] In some embodiments, the skin topical agent is selected from: facial cleanser, toner, lotion, cream, gel, and facial mask. Different amounts are added according to different types of preparations.

[0037] The skin external preparation is a general concept for all ingredients usually used on the outside of the skin, for example, it can be a cosmetic composition. The cosmetic composition can be a basic cosmetic, a facial makeup cosmetic, a body cosmetic, a hair care cosmetic, etc., and there is no special restriction on its dosage form, which can be reasonably selected according to different purposes. The cosmetic composition also contains different media or matrix excipients allowed by the cosmetics level according to different dosage forms and purposes.

[0038] The topical preparation for skin contains a dermatologically acceptable carrier or vehicle (eg, lotion, cream, ointment, cleanser, etc.). A person skilled in the art can select a carrier that can dissolve or disperse these components at the above-mentioned concentrations according to common knowledge in the art.

[0039] Those skilled in the art can select suitable carriers, such as water, alcohols, oils, etc., based on common knowledge and their ability to dissolve or disperse in the active ingredient at the concentration most suitable for the active ingredient during treatment.

[0040] Skin external preparation of the present invention can be the form of local application product, and it can be applied on the skin from the outside, and can be prepared with those common techniques well known in the art.Carrier can have various actual forms, for example facial cream, dressing, gel, washing liquid, ointment or liquid, it comprises the composition of applying and washing off, and joins them in for example dry or wet smear, hydrogel matrix, or the material carrier of viscous (or non-sticky) patch with method well known in the art.Preferably, carrier is a kind of gel or the lotion that increases moisture, or with the smear of dry or wet form.

[0041] Typical carriers include emulsions containing water and / or alcohol and emollients, such as hydrocarbon oils and waxes, silicone oils, hyaluronic acid, plant, animal or marine fats or oils, glyceride derivatives, fatty acids, or fatty acid esters or alcohols or alcohol ethers, lanolin and its derivatives, polyols or esters, wax esters, sterols, phospholipids, etc., generally with emulsifiers (nonionic, cationic or anionic), although some emollients have emulsifying properties themselves. In addition, these same components can be formulated into creams, gels, or solid sticks using different proportions of their components and / or by incorporating thickeners such as gums or other forms of hydrophilic colloids.

[0042] The skin external preparation of the present invention may contain additional components commonly found in skin care compositions, such as emollients, skin regulators, emulsifiers, preservatives, antioxidants, fragrances, chelating agents, etc., as long as they are physically and chemically compatible with other components in the skin external preparation and do not affect the effects of the plant seed oil of the present invention.

[0043] In some embodiments of the skin topical preparation of the present invention, one or more preservatives may be used. Suitable preservatives include p-hydroxyacetophenone, C1-C4 alkyl parahydroxybenzoate and phenoxyethanol. Based on the total weight of the composition, the amount of the preservative is about 0.5 to about 2 weight %, preferably about 0.5 to 1 weight %.

[0044] In one example of the skin external preparation of the present invention, one or more antioxidants can be used. Suitable antioxidants include butylated hydroxytoluene (BHT), ascorbyl palmitate (BHA), butylated hydroxyanisole, phenyl-α-naphthylamine, hydroquinone, propyl gallate, nordihydroguaiaretic acid, vitamin E or vitamin E derivatives, vitamin C and its derivatives, calcium pantothenate, green tea extract and mixed polyphenols, and mixtures of the above-mentioned substances. The antioxidant used is about 0.02 to 0.5 weight % of the total weight of the composition, and more preferably from about 0.002 to 0.1 weight % of the amount used.

[0045] In one example of the skin external preparation of the present invention, one or more emollients can be used to act as lubricants through their ability to remain on the skin surface or in the stratum corneum to reduce flaking and improve the appearance of the skin. Typical emollients include fatty esters, fatty alcohols, mineral oils, polyether silicone copolymers, and the like. Examples of suitable emollients include, but are not limited to, polypropylene glycol ("PPG")-15 stearyl ether, PPG-10 cetyl ether, Steareth-10, Oleth-8, PPG-4 lauryl ether, vitamin E acetate, lanolin, cetyl alcohol, cetearyl ethylhexanoate, cetearyl alcohol, glyceryl stearate, hydroxystearate octyl, dimethyl polysiloxane, and combinations thereof. Cetyl alcohol, cetearyl ethylhexanoate, cetearyl alcohol, glyceryl stearate, and combinations thereof are preferred. When used, the emollient is used in an amount ranging from about 0.1 to about 30% by weight, preferably about 1 to about 30% by weight, based on the total weight of the composition.

[0046] In one example of the skin external preparation of the present invention, one or more moisturizers may also be used. Moisturizers are also called humectants, which help to improve the effect of emollients, reduce flaking, remove the scales of the composition and improve the touch of the skin. Polyols can be used as moisturizers, including but not limited to glycerol, polyalkylene glycols, alkylene polyols and derivatives thereof, including butylene glycol, propylene glycol, dipropylene glycol, polyglycerol, polyethylene glycol and derivatives thereof, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1,3-dibutylene glycol, 1,2,6-hexanetriol, ethoxylated glycerol, propoxylated glycerol, and combinations thereof. When used, the amount of moisturizer is about 0.1 to about 20% by weight, preferably about 1 to about 15% by weight, based on the total weight of the composition.

[0047] In an example of the skin external preparation of the present invention, one or more emulsifiers can be used. Emulsifiers can be used within the scope of effective stabilizing amount. Preferably, based on the total weight of the composition, the emulsifier is used in an amount of about 1.0 to about 10.0 weight %, more preferably about 3.0 to about 6.0 weight %. Any emulsifier compatible with the components in the composition can be used. Suitable emulsifiers include stearic acid, cetyl alcohol, glyceryl stearate, lecithin, stearyl alcohol, Steareth-2, Steareth-20, acrylic acid (ester) class / C10-30 alkyl alcohol acrylate cross-linked polymer, and combinations thereof.

[0048] In one example of the skin external preparation of the present invention, one or more pH regulators can be used. The pH regulator useful in the skin external preparation of the present invention includes tromethamine. When used, the amount of the pH regulator based on the total weight of the composition is about 0.1 to about 2 weight %, preferably about 0.1 to about 1 weight %.

[0049] In a specific embodiment of the present invention, the skin topical agent comprises acrylates / C10-30 alkyl acrylate crosspolymer, glycerin, p-hydroxyacetophenone, glyceryl stearate and lecithin, cetearyl alcohol, cetearyl ethylhexanoate, tromethamine or a combination thereof.

[0050] In some embodiments of the present invention, the composition of the present invention is used in an external skin preparation in an amount of 0.001%-100% (w / w), preferably in an amount of 0.01%-60% (w / w), and more preferably in an amount of 0.01%-40% (w / w).

[0051] Example

[0052] The present invention is further described below in conjunction with specific examples. It is necessary to point out that the examples are only used to further illustrate the present invention and cannot be construed as limiting the scope of the present invention. Those skilled in the art can make some non-essential improvements and adjustments based on the content of the present invention described above. The test methods in the following examples that do not specify specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise stated, all percentages and parts are by weight.

[0053] The experimental materials used in the following examples include:

[0054] Jojoba (Simmondsia chinensis) seed oil, trade name jojoba oil, purchased from Vantage Specialty Ingredients, Inc.

[0055] Safflower (Carthamus tinctorius) seed oil, trade name safflower seed oil, purchased from Northstar Lipids UK Ltd.

[0056] Limnanthes alba seed oil, trade name Limnanthes alba seed oil, purchased from Hemmings Specialty Chemicals;

[0057] Sunflower (Helianthus annuus) seed oil, trade name sunflower seed oil, purchased from Cargill Grain and Oil (Nantong) Co., Ltd.;

[0058] Hydrogenated lecithin, trade name hydrogenated lecithin, purchased from NIKKO CHEMICALS CO., LTD.

[0059] The experimental instruments used in the embodiment include:

[0060] Weighing balance: METTLER TOLEDO PB4002-N;

[0061] pH meter: METTLER TOLEDO SEVENMULTI type;

[0062] Water bath: HWS28 electric constant temperature water bath produced by Shanghai Yiheng Technology Co., Ltd.

[0063] Homogenizer: POLYTRON PT 3100D;

[0064] Table mixer: IKA @ EUROSTAR,power control-visc..

[0065] Example 1: Preparation of composition

[0066] Weigh 0.5 parts by mass of hydrogenated lecithin into a beaker, add 99.5 parts by mass of deionized water, stir evenly, and set aside.

[0067] Example 2: Preparation of a composition comprising plant seed oils

[0068] Weigh 0.5 parts by mass of hydrogenated lecithin and 0.5 parts by mass of jojoba oil into a beaker, heat to 75-80°C while stirring, then weigh 99 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0069] Example 3: Preparation of a composition comprising plant seed oils

[0070] Weigh 0.5 parts by mass of hydrogenated lecithin and 2.5 parts by mass of jojoba oil into a beaker, heat to 75-80°C while stirring, then weigh 97 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0071] Example 4: Preparation of a composition comprising plant seed oils

[0072] Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of jojoba oil into a beaker, heat to 75-80°C while stirring, then weigh 94.5 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0073] Example 5: Preparation of a composition comprising plant seed oils

[0074] Weigh 0.5 parts by mass of hydrogenated lecithin and 7.5 parts by mass of jojoba oil into a beaker, heat to 75-80°C while stirring, then weigh 92.5 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0075] Example 6: Preparation of a composition comprising plant seed oils

[0076] Weigh 0.5 parts by mass of hydrogenated lecithin and 10 parts by mass of jojoba oil into a beaker, heat to 75-80°C while stirring, then weigh 89.5 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0077] Example 7: Preparation of a composition comprising plant seed oils

[0078] Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of safflower seed oil into a beaker, heat to 75-80°C while stirring, then weigh 94.5 parts by mass of deionized water at 75-80°C into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0079] Example 8: Preparation of a composition comprising plant seed oils

[0080] Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of sunflower seed oil into a beaker, heat to 75-80° C. while stirring, then weigh 94.5 parts by mass of deionized water at 75-80° C. into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0081] Example 9: Preparation of a composition comprising plant seed oils

[0082] Weigh 0.5 parts by mass of hydrogenated lecithin and 5 parts by mass of meadowfoam seed oil into a beaker, heat to 75-80° C. while stirring, then weigh 94.5 parts by mass of deionized water at 75-80° C. into the beaker, start homogenization at a speed of 3000 rpm, homogenize for 5-10 minutes, and set aside.

[0083] Example 10: Preparation of a composition comprising plant seed oils

[0084] Weigh 100 parts by mass of jojoba oil for testing.

[0085] Example 11: Preparation of a composition comprising plant seed oils

[0086] Weigh 100 parts by mass of safflower seed oil for testing.

[0087] Test Example 1: 3D epidermal reconstruction model tissue vitality test

[0088] The 3D epidermal reconstruction model is a human 3D epidermal model that can simulate the structure of each layer of the epidermis differentiated by keratinocytes. The 3D epidermal reconstruction model tissue vitality test method adopted by the present invention first causes certain damage to the model with a skin irritant SDS, and then covers the surface of the damaged model with an appropriate amount of the sample prepared in the embodiment for 24 hours for repair. After 24 hours, the sample is washed off and the tissue vitality recovery is tested by the MTT method. According to the tissue vitality recovery, the skin barrier repair ability of the sample in the embodiment can be evaluated.

[0089] 1. Test materials

[0090] 1.1 Cell: The skin model is a 3D epidermal skin model (EpiSkin).

[0091] 1.2 Reagents:

[0092] 3D skin model maintenance medium, 3D skin model detection medium (EpiSkin); sodium dodecyl sulfate (SDS) (Sigma); DPBS containing calcium and magnesium (Gibco); MTT (Sigma); isopropanol and concentrated hydrochloric acid (Sinopharm Group).

[0093] 1.3 Main Equipment

[0094] CO 2 Incubator HERAcell 240i, biosafety cabinet 1300SERIES A2, microplate reader Multiskan Mk3 (Thermo); forward pipette M25 (Gilson); 50mL continuous pipette tip Multipette plus (Eppendorf); skin punch (area 0.38cm2 ).

[0095] 2. Experimental methods:

[0096] 3D reconstructed epidermal model reception and recovery

[0097] Receive the 3D epidermal reconstruction model, check the packaging integrity, check the number of models, check the maintenance culture medium, and check the color and volume of the culture medium. After confirmation, start the model recovery.

[0098] Perform the recovery operation in a biosafety cabinet. Take a certain number of 12-well plates, add 2 ml / well of maintenance medium in the first row, then take out the 3D reconstructed epidermal model with tweezers, carefully remove the surface gel with a cotton swab, and put it into the well containing maintenance medium for 24 hours. One 12-well plate can recover 4 3D reconstructed epidermal models. Culture conditions: 5% CO 2 , cultured in a 37℃ constant temperature incubator.

[0099] 3D reconstructed epidermal model injury treatment and sample addition

[0100] A 1.5% (mass-volume ratio) sodium dodecyl sulfate (SDS) solution was prepared using 1× phosphate buffered saline (DPBS) solution.

[0101] The model was divided into a blank control group (no treatment, 3 replicates), a negative control group (damage treatment with 1.5% SDS, 3 replicates), and sample groups of Examples 1-6 and Examples 10-11 (each sample 3 replicates).

[0102] First, add 2 ml / well of maintenance medium to the second row of the 12-well plate, and then use forceps to transfer the 3D reconstructed epidermis model in the first row to the corresponding second row.

[0103] Use a pipette to drip 50 microliters of 1.5% SDS solution on the surface of the negative control group and the sample groups of Examples 1-6 and 8-9, and ensure uniform coverage, and incubate at room temperature for 15 minutes. After 15 minutes, immediately wash with 1×DPBS, and gently wipe the residual liquid with a cotton swab. Then use a piston displacement pipette (M25) to take 15 microliters of sample and apply it on the surface of the sample group model that has been damaged (1.5% SDS) for 24 hours. All models are placed in a cell culture incubator, and the culture conditions are: 5% CO 2 , cultured in a 37℃ constant temperature incubator.

[0104] 3D reconstructed epidermal model vitality test

[0105] Prepare 3 mg / ml Thiazole Blue (MTT) stock solution in 1×DPBS, and dilute the MTT stock solution to 0.3 mg / ml in the test medium. Add 2 ml / well of the diluted MTT solution to the third row of the 12-well plate.

[0106] Use a 50 ml continuous pipette to absorb 1× DPBS and rinse the sample on the surface of the model (do not rinse directly on the sample, but on the side wall of the skin model support frame), then use a cotton swab to absorb the remaining liquid and put it into the MTT solution in the third row of the 12-well plate. Finally, put it in a cell culture incubator at 37°C and 5% CO. 2 Incubate in an incubator for 3 hours.

[0107] To prepare acidic isopropanol, add 1.8 ml of hydrochloric acid to 500 ml of isopropanol. Take the corresponding number of 1.5 ml small centrifuge tubes (EP tubes) of the model and add 500 μl of acidic isopropanol to each tube. After the epidermal tissue is incubated in MTT for 3 hours, remove the epidermal tissue with a hole punch, separate the epidermal tissue from the collagen base underneath with two small elbow tweezers, turn the epidermal tissue over, and then transfer the epidermal tissue and the collagen base together to the 1.5 ml EP tube containing acidic isopropanol. Shake the EP tube on a vortex to mix well and place it in a 4°C refrigerator for 72 hours for color extraction. At the same time, prepare an EP tube containing 1 ml of acidic isopropanol as a blank well for microplate reader detection.

[0108] Take out the 1.5 ml EP tube containing acidic isopropanol and skin model from the 4°C refrigerator, shake and mix the solution, take 150 μl of the extraction solution from each tube and transfer it to a 96-well plate, take it 3 times and add it to the 96-well plate. At the same time, prepare 3-6 wells of acidic isopropanol as a blank control during the detection to exclude the background optical density value (OD value). Read the OD value at a wavelength of 570 nanometers. Calculate the relative activity of each group of models according to the following formula (the blank control group is 100%):

[0109]

[0110] 3. Result judgment

[0111] The blank control group was an undamaged group with a relative activity of 100%; the relative activity of the negative control group was controlled at 40-60% compared with the blank control group; the relative activity of the sample group was higher than that of the negative control group, indicating that the sample group had the ability to promote barrier repair, and the relative activity of the sample group was lower than that of the negative control group, indicating that the sample group did not have the ability to promote barrier repair.

[0112] Table 1: Relative activity of tissues in each group detected by MTT method

[0113]

[0114] As shown in Table 1, the average relative activity of the skin model tissue of the blank control group was 100%, and the average relative activity of the skin model tissue of the negative control group (1.5% SDS treatment) was 41.0%. The average relative activity of the skin model tissue of the sample of Example 1 (0% jojoba oil) was 48.4%, the average relative activity of the skin model tissue of the sample of Example 2 (0.5% jojoba oil) was 60.6%, the average relative activity of the skin model tissue of the sample of Example 3 (2.5% jojoba oil) was 57.0%, the average relative activity of the skin model tissue of the sample of Example 4 (5% jojoba oil) was 66.9%, the average relative activity of the skin model tissue of the sample of Example 5 (7.5% jojoba oil) was 68.9%, the average relative activity of the skin model tissue of the sample of Example 6 (10% jojoba oil) was 63.0%, the average relative activity of the skin model tissue of the sample of Example 10 (100% jojoba oil) was 65.2%, and the average relative activity of the skin model tissue of the sample of Example 11 (100% safflower oil) was 70.8% (see Table 1).

[0115] In summary, the jojoba oil in Examples 2-6 and 10 has good tissue repair ability within the concentration range of 0.5-100%.

[0116] Test Example 2: 3D reconstructed skin epidermis model barrier-related gene expression test

[0117] The 3D epidermal reconstruction model is a human 3D epidermal model that can simulate the structure of each layer of the epidermis differentiated by keratinocytes. An appropriate amount of sample is covered on the epidermal model for 24 hours, and then the total RNA (RNA) of the epidermal tissue is collected. The skin barrier-related genes are quantitatively detected using RNA reverse transcription technology and gene microarray technology to evaluate the sample's skin barrier moisturizing and repair capabilities.

[0118] 1. Test materials

[0119] 1.1 Cell: The skin model is a 3D epidermal skin model (EpiSkin).

[0120] 1.2 Reagents:

[0121] 3D skin model maintenance medium (EpiSkin); calcium-magnesium-containing DPBS (Gibco); isopropanol, 75% anhydrous ethanol (Sinopharm Group); DEPC water (Tiangen Biotechnology); total RNA extraction reagent TRIzol (ThermoFisher); reverse transcription kit iScript cDNA Synthesis Kit, fluorescent real-time quantitative PCR reagent iTaq TM Universal Green Supermix (Bio-rad).

[0122] 1.3 Main Equipment

[0123] CO 2 Incubator HERAcell 240i, biosafety cabinet 1300SERIES A2, ultra-micro-volume UV spectrophotometer NanoDrop ONE (Thermo); gene amplifier C1000 Touch, real-time quantitative PCR instrument CFX Connect (Bio-rad); high-speed refrigerated centrifuge MIKRO 220R (Hittich); forward pipette M25 (Gilson); 50mL continuous sampler tip Multipette plus (Eppendorf); skin punch (area 0.38cm 2 ).

[0124] 2. Experimental methods:

[0125] 3D reconstructed epidermal model reception and recovery

[0126] Receive the 3D epidermal reconstruction model, check the packaging integrity, check the number of models, check the maintenance culture medium, and check the color and volume of the culture medium. After confirmation, start the model recovery.

[0127] Perform the recovery operation in a biosafety cabinet. Take a certain number of 12-well plates, add 2 ml / well of maintenance medium in the first row, then take out the 3D reconstructed epidermal model with tweezers, carefully remove the surface gel with a cotton swab, and put it into the well containing maintenance medium for 24 hours. One 12-well plate can recover 4 3D reconstructed epidermal models. Culture conditions: 5% CO 2 , cultured in a 37℃ constant temperature incubator.

[0128] 3D reconstructed epidermal model sample processing

[0129] The model was divided into sample groups of Examples 1, 4, 7-9 (each sample had 3 replicates) and a positive control group (rosiglitazone, 3 replicates).

[0130] First, add 2 ml / well of maintenance medium to the second row of the 12-well plate, and then use forceps to transfer the 3D reconstructed epidermis model in the first row to the corresponding second row.

[0131] Use a piston displacement pipette (M25) to add 15 μl of rosiglitazone solution (100 μM, dissolved in 1× DPBS) to the positive control group, add 15 μl of sample to the sample group, and use a nozzle to gently apply the sample evenly on the surface of the skin model. Repeat three times for each sample. All models were placed in a cell culture incubator and cultured for 24 hours. Culture conditions: 5% CO 2 , cultured in a 37℃ constant temperature incubator.

[0132] 3D reconstructed epidermal model to collect total RNA samples

[0133] Use a 50 ml continuous pipette to absorb 1×DPBS, rinse the sample on the surface of the model (do not rinse directly at the sample, but at the side wall of the skin model support frame), and then use a cotton swab to absorb the residual liquid. Cut the epidermal tissue with a punch, and use two small elbow tweezers to separate the epidermal tissue from the collagen base underneath. Put the epidermal tissue into an EP tube containing 1 ml of total RNA extraction reagent (TRIzol), and use a 1 ml RNase-free pipette nozzle to repeatedly blow the epidermal tissue until only the transparent stratum corneum remains. Subsequently, add 0.2 ml of chloroform to the original EP tube, shake vigorously, and centrifuge at low temperature and high speed for 15 minutes. Use an RNase-free pipette nozzle to take 500 μl of the upper aqueous phase, then add an equal volume of pre-cooled (minus 20 degrees) isopropanol, invert and mix, and centrifuge at low temperature and high speed for 10 minutes. After centrifugation, the RNA forms a white flake precipitate at the bottom of the EP tube. Rinse it twice with 75% ethanol, remove the residual ethanol with a centrifuge and a pipette, open the lid and let it dry at room temperature until it becomes transparent. 2 O to dissolve and mix. Then use the micro-spectrophotometer Nanodrop to measure the RNA concentration, and use agarose gel electrophoresis to detect the integrity of the RNA. The dissolved total RNA of the epidermal model tissue can be used for subsequent experiments or stored at -80℃ for a long time after aliquoting.

[0134] Quantitative detection of skin barrier-related genes by RT-qPCR

[0135] One microgram of total RNA was taken for reverse transcription, and cDNA was obtained by reverse transcription using the iScript cDNA Synthesis Kit.

[0136] The reverse transcription system is as follows:

[0137]

[0138] The reverse transcription program was as follows: 25°C, 5 min; 42°C, 30 min; 85°C, 5 min.

[0139] iTaq real-time quantitative polymerase chain reaction (PCR) reagent TM Universal GreenSupermix was used for real-time fluorescence quantitative detection to analyze the expression of barrier-related gene messenger RNA (mRNA).

[0140] The reaction system is as follows:

[0141]

[0142]

[0143] The reaction program was as follows: 95°C, 30 seconds; 95°C, 15 seconds, 60°C, 30 seconds, 40 cycles; 65-95°C, decreasing by 0.5°C each time (melting curve).

[0144] The primer sequences used in this example are designed as follows:

[0145]

[0146] use The gene expression of the sample group and the positive control group (rosiglitazone) in the example was analyzed relative to the gene changes of the control group in Example 1.

[0147] 3. Result judgment

[0148] The gene expression of the sample group in Example 1 is taken as 100%, and the gene expression of each group is greater than 100%, indicating that the gene expression is upregulated, and less than 100%, indicating that the gene expression is downregulated. The upregulation of genes such as FLG and LOR indicates that the sample group has the ability to moisturize and promote barrier repair; the downregulation of genes such as FLG and LOR indicates that the sample group does not have the ability to moisturize and promote barrier repair.

[0149] Table 2: Expression of skin barrier factors after treatment of each group of samples

[0150]

[0151] As shown in Table 2, Example 1 (0% jojoba oil) was used as a control, and its relative gene expression was 100%. The gene expression of the samples of Examples 4, 7, 8 and 9 and the positive control sample was compared with it, and the following percentages were obtained.

[0152] Specifically, the barrier factor FLG after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) was 218%, 274%, 283%, 175% and 362%, respectively, compared with the sample of Example 1 (0% jojoba oil).

[0153] Specifically, the barrier factor LOR after treatment with the samples of Example 4 (5% jojoba oil), Example 7 (5% safflower oil), Example 8 (5% sunflower oil), Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) were 236%, 307%, 293%, 201% and 351%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0154] Specifically, the barrier factor IVL after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) were 87%, 117%, 90%, 103% and 103%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0155] Specifically, the barrier factor TGM1 after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) was 126%, 201%, 146%, 149% and 161%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0156] Specifically, the barrier factor CASP14 after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) were 275%, 493%, 248%, 279% and 330%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0157] Specifically, the barrier factor HMGCR after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) was 223%, 242%, 202%, 175% and 229%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0158] Specifically, the barrier factor AQP3 after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) were 105%, 148%, 116%, 97% and 131%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0159] Specifically, the barrier factor ZO-1 after treatment with the sample of Example 4 (5% jojoba oil), the sample of Example 7 (5% safflower oil), the sample of Example 8 (5% sunflower oil), the sample of Example 9 (5% meadowfoam oil) and the positive control (rosiglitazone) was 151%, 203%, 170%, 134% and 246%, respectively, compared with the sample of Example 1 (control group: 0% jojoba oil).

[0160] In summary, the sample in Example 4 (5% jojoba oil) promotes the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is conducive to barrier formation, and has the effect of promoting skin moisturizing and barrier repair. The sample in Example 7 (5% safflower oil) promotes the expression of FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is conducive to barrier formation, and has the effect of promoting skin moisturizing and barrier repair. The sample in Example 8 (5% sunflower seed oil) promotes the expression of FLG, LOR, TGM1, CASP14, HMGCR, AQP3 and ZO-1 barrier factors, which is conducive to barrier formation, and has the effect of promoting skin moisturizing and barrier repair. The sample in Example 9 (5% white pondweed seed oil) promotes the expression of FLG, LOR, TGM1, CASP14, HMGCR and ZO-1 barrier factors, which is conducive to barrier formation, and has the effect of promoting skin moisturizing and barrier repair.

[0161] Application Examples

[0162] The composition containing plant seed oils and fats described in the present invention can be used as an intermediate raw material or functional additive for the preparation of medicines or external skin preparations. The external skin preparations are preferably cosmetic compositions, including but not limited to the preparation of products in dosage forms such as creams, lotions, gels, toners, essences, facial masks, eye creams, aerosols (cleansing bubbles), sprays, shower gels, massage oils, and facial cleansers.

[0163] The weight percentage of the composition containing plant fruit oil in the skin external preparation of the present invention is 0.001%-100% (w / w); the preferred weight percentage is 0.01%-60% (w / w); and the more preferred weight percentage is 0.01%-40% (w / w).

[0164] The following are specific application examples of the composition containing plant seed oil obtained in Examples 2, 3, 5, 8 and 9 in skin external preparations, as well as the formulations and preparation methods of these dosage forms. The specific application examples are as follows:

[0165] Application Example 1: Preparation of facial cream

[0166]

[0167] Application Example 2: Preparation of Emulsion

[0168]

[0169]

[0170] Application Example 3: Preparation of Eye Cream

[0171]

[0172]

[0173] Application Example 4: Preparation of facial mask

[0174]

[0175] Application Example 5: Preparation of Essence

[0176]

[0177] Application Example 6: Examples 2-11 of the present invention can also be used alone as a skin external preparation.

Claims

1. Use of a composition comprising plant seed oil for enhancing skin barrier function for non-therapeutic purposes, wherein the enhancing skin barrier function is achieved by enhancing skin barrier activity and / or promoting the expression of skin barrier-related factors, in, The plant seed oil is selected from jojoba seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil or a combination thereof, and the composition comprises at least 0.5% by weight of the plant seed oil based on the total weight of the composition. Wherein, the composition further comprises a natural surfactant, Wherein, the weight ratio of the plant seed oil to the natural surfactant is 10:1 to 20:

1.

2. The use according to claim 1, wherein: The natural surfactant is selected from the group consisting of lecithin, cholesterol, lanolin, tea saponin, protein, saponin, sugar, alkyl polyglycoside or a combination thereof.

3. The use according to claim 2, wherein: The natural surfactant is hydrogenated lecithin.

4. The use according to claim 1, wherein: The skin barrier-related factors are selected from: FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1.

5. Use of the composition according to claim 1 in the preparation of a medicine and / or a skin external preparation for enhancing skin barrier function, wherein the enhancing skin barrier function is achieved by enhancing skin barrier activity and / or promoting the expression of skin barrier-related factors, in, The plant seed oil is selected from jojoba seed oil, safflower seed oil, meadowfoam seed oil, sunflower seed oil or a combination thereof, and the composition comprises at least 0.5% by weight of the plant seed oil based on the total weight of the composition. Wherein, the composition further comprises a natural surfactant, Wherein, the weight ratio of the plant seed oil to the natural surfactant is 10:1 to 20:

1.

6. The use according to claim 5, wherein: The natural surfactant is selected from the group consisting of lecithin, cholesterol, lanolin, tea saponin, protein, saponin, sugar, alkyl polyglycoside or a combination thereof.

7. The use according to claim 6, wherein: The natural surfactant is hydrogenated lecithin.

8. The use according to claim 5, wherein the medicine and / or external skin preparation comprises 0.001-100% by weight of the composition.

9. The use according to claim 5, wherein: The skin barrier-related factors are selected from: FLG, LOR, IVL, TGM1, CASP14, HMGCR, AQP3 and ZO-1.

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