Exosome compound for hair growth, hair growth liquid and application thereof

Abstract

The invention relates to an exosome compound for hair growth, hair growth liquid and application thereof, and belongs to the technical field of hair growth preparations, the exosome compound comprises an exosome composition, the exosome composition comprises hair follicle stem cell exosomes and ginseng exosomes, the mass ratio of the ginseng exosomes to the hair follicle stem cell exosomes is 1: (1.5-3), and the exosome composition further comprises a polypeptide composition, the exosome composition is 20-35 parts by mass, and the polypeptide composition is 8-15 parts by mass. According to the exosome compound for hair growth, the ginseng exosome and the hair follicle stem cell exosome are jointly applied to the field of hair growth, concentration ratio model verification is carried out, compared with a single exosome, the ginseng exosome and the hair follicle stem cell exosome are jointly used for treating alopecia, the effect of inhibiting inflammation is achieved, meanwhile, proliferation of hair papilla cells can be promoted, and the hair growth rate is increased. Compared with the method for treating alopecia through operation, the method has the advantages of being low in immunogenicity, convenient to operate and the like, and is better in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of hair growth preparations, and in particular to an exosome complex for hair growth, a hair growth liquid and applications thereof. Background Art

[0002] Hair loss is a common dermatological disease, and it is becoming younger. In addition to genetic factors, excessive mental stress, endocrine disorders, inflammatory infections, seborrheic alopecia, etc. all dominate or interact with each other to varying degrees to affect the process of hair loss. The most common clinical condition is androgenic alopecia, also known as seborrheic dermatitis. This is because the hair follicles on the scalp are sensitive to dihydrotestosterone (DHT), a metabolite of androgens in the body, which can bind to the hair follicles and cause them to atrophy, gradually shortening the hair growth period, and eventually causing it to stop growing and fall out. This condition is the result of the combined effects of androgen metabolites, abnormal lipid metabolism, and scalp inflammation.

[0003] Although there are currently a variety of solutions for the treatment of hair loss, including oral or topical medication, laser, surgical hair transplantation, etc., each method has certain disadvantages in terms of effectiveness, safety, convenience, etc. Among them, minoxidil has been used to treat hair loss for more than 30 years. It usually takes 6-9 months for topical minoxidil to show significant effects, and the effective rate can reach 50% to 85%. However, while minoxidil is effective, it also has side effects. The first is the crazy shedding period in the early stage of medication, and the second is the recurrence of shedding after minoxidil is discontinued. Finasteride inhibits 5α-reductase and reduces the production of dihydrotestosterone, effectively blocking the damage to hair follicles. Generally, hair loss decreases after taking the medicine for 3 months, and the effective rate can reach 65% to 90% after 1 year of medication. However, finasteride is not suitable for women and is limited in its application in the treatment of hair loss. Therefore, it is urgent to develop a hair growth preparation that integrates application effect, safety and convenience to improve the existing hair growth methods. Summary of the invention

[0004] The purpose of the present invention is to provide an exosome complex for hair growth, a hair growth liquid and applications thereof, which can solve the technical problems existing in the traditional hair loss treatment mentioned in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A first aspect of the present invention provides an exosome complex for hair growth, comprising an exosome composition, wherein the exosome composition comprises hair follicle stem cell exosomes and ginseng exosomes.

[0007] In a preferred embodiment, in the exosome composition, the mass ratio of the ginseng exosomes to the hair follicle stem cell exosomes is 1:(1.5-3).

[0008] In a preferred embodiment, the mass ratio of the ginseng exosomes to the hair follicle stem cell exosomes is 1:(2-3).

[0009] In a preferred embodiment, the concentration range of the hair follicle stem cell exosomes is:

[0010] 3E+10~1E+11particle / mL.

[0011] In a preferred embodiment, the concentration of the ginseng exosomes is greater than 3E+10particle / mL.

[0012] In a preferred embodiment, the exosome complex further comprises a polypeptide composition, and in terms of weight fractions, the exosome composition is 20-35 parts, and the polypeptide composition is 8-15 parts.

[0013] In a preferred embodiment, the polypeptide composition comprises: a combination of one or more of copper peptide, acetyl tetrapeptide-3, and oligopeptide-54.

[0014] The second aspect of the present invention provides a hair growth liquid containing the exosome complex for hair growth described in any of the above schemes.

[0015] In a preferred embodiment, the content of the exosome complex in the hair growth fluid is 3-15 wt %.

[0016] The third aspect of the present invention provides the use of the exosome complex or the hair growth liquid in hair growth, hair growth, and hair loss prevention products.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: the exosome complex for hair growth provided by the present invention combines ginseng exosomes and hair follicle stem cell exosomes in the field of hair growth, and performs model verification of concentration ratio. Compared with the use of a single exosome, the combination of the two for the treatment of hair loss has the effect of inhibiting inflammation, while promoting the proliferation of hair papilla cells and reducing the damage to hair follicles caused by abnormal androgen metabolism. Compared with surgical treatment of hair loss, it has the advantages of low immunogenicity and convenient operation, and is safer.

[0018] Further use together with the hair growth active polypeptide complex can exert its effect in different cells and at different times, synergistically promoting the hair growth effect, and has a more significant hair growth effect in model verification.

[0019] The exosome complex for hair growth provided by the present invention has various and convenient administration routes, and has been experimentally verified to significantly promote the proliferation of hair papilla cells in male pattern baldness and inflammation models. Therefore, it can effectively target people with diseases such as androgenic alopecia, inflammatory alopecia and seborrheic alopecia. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a comparison diagram of the promoting effects of hair follicle stem cell exosomes of different concentrations on the proliferation activity of hair papilla cells in an embodiment of the present invention;

[0021] Figure 2 This is a comparison chart of the regulatory effects of hair follicle stem cell exosomes of different concentrations on the growth factor VEGF in an embodiment of the present invention;

[0022] Figure 3 This is a comparison diagram of the regulatory effects of hair follicle stem cell exosomes of different concentrations on the Wnt / β-catenin signaling pathway in an embodiment of the present invention;

[0023] Figure 4 This is a comparison chart of the inhibitory effects of ginseng exosomes at different concentrations on IL-6 inflammatory factors in the embodiments of the present invention;

[0024] Figure 5 This is a comparison diagram of the regulatory effects of ginseng exosomes at different concentrations on the Wnt / β-catenin signaling pathway in the embodiments of the present invention;

[0025] Figure 6 This is a comparison chart of the promoting effect of the exosome complexes provided in various embodiments and comparative examples of the present invention on the proliferation activity of dermal papilla cells. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The present invention provides an exosome complex for hair growth, comprising plant exosomes and human stem cell exosomes, wherein the plant exosomes are ginseng exosomes, and the human stem cell exosomes are hair follicle stem cell exosomes.

[0028] Hair follicle stem cell exosomes contain a variety of biomacromolecules, which can affect the MAPK signaling pathway, Wnt signaling pathway, etc., and then regulate a series of important biological processes such as osteogenic differentiation of adipose stem cells, promote wound healing of dermal fibroblasts and promote hair regeneration. Specifically, the morphogenesis and development of hair follicles are regulated by multiple signals, among which the Wnt / β-catenin signal regulates the formation of hair follicle placodes, hair growth cycles, differentiation of hair follicle stem cells and other stages. It is also called the first signal to start hair development and is a necessary and sufficient condition for hair follicle growth. In addition, after the Wnt3a / β-catenin signaling pathway in hair matrix cells is activated, it forms a complex with TCF4, which promotes the proliferation of dermal papilla cells by upregulating the expression of proliferation-related factors such as LEF-1, VEGF, IGF-1, Ki67 and TGF-β, thereby inducing hair follicle regeneration. Failure or abnormality of the Wnt signaling pathway in hair follicle stem cells not only prevents hair growth, but also inhibits the physiological function of melanocyte stem cells related to pigment formation, causing hair to stop growing, fall off or turn white. At the same time, the growth of hair follicles is also affected by the MAPK signaling pathway, which can regulate the growth of hair follicles by regulating the secretion of growth factors such as VEGF and activating the hair follicle growth cycle. Specifically, the applicant found that hair follicle stem cell exosomes can promote the proliferation of hair papilla cells in the androgen model and affect the expression of VEGF and β-catenin, which indicates that hair follicle stem cell exosomes can promote the proliferation of hair papilla cells, while effectively regulating the Wnt / β-catenin pathway related to hair growth, regulating the expression of growth factors such as VEGF, thereby promoting the activation of hair follicle stem cells and inducing hair follicle regeneration.

[0029] Furthermore, ginseng exosomes can maintain the morphology and activity of dermal papilla cells. These processes are the result of the interconnection, mutual influence and joint regulation of multiple signal transduction pathways and gene families, such as the Wnt / β-catenin-TCF4 signaling pathway, Cdx1 / Cdx2 family, Math1 gene, etc., among which the Wnt / β-catenin pathway is the most significantly regulated.

[0030] The applicant verified ginseng exosomes in an inflammatory cell model and found that ginseng exosomes can partially enhance the proliferation activity of hair papilla cells in an LPS (lipopolysaccharide)-induced inflammatory model and significantly reduce the expression of the inflammatory factor IL-6. In the androgen model, ginseng exosomes can also increase the expression of β-catenin, which suggests that ginseng exosomes and hair follicle stem cell exosomes are coordinated in the Wnt / β-catenin signaling pathway and work together to promote hair growth. At the same time, ginseng exosomes can significantly increase the expression of proliferation-related factors Sox9, Ki67 and TGF-β1 in keratinocytes, indicating that ginseng exosomes regulate the growth of hair follicles by promoting the proliferation and differentiation of hair papilla cells.

[0031] The ginseng exosomes and hair follicle stem exosomes verified by the present invention have been screened and verified by models, and can provide a solution for multi-factor interference with hair loss, including but not limited to androgenic alopecia, seborrheic alopecia, and inflammatory alopecia. They can target the atrophied and degenerated hair follicles in the resting phase and allow dormant hair follicles to resume the growth phase.

[0032] Preferably, in the exosome complex for hair growth provided by the present invention, the mass ratio of ginseng exosomes to hair follicle stem cell exosomes is 1:(1.5-3). Within this ratio range, ginseng exosomes and hair follicle stem cell exosomes can more significantly promote the proliferation of hair papilla cells and the secretion of growth factor VEGF in androgen models and inflammatory models, and have a better effect on promoting hair growth. Because there is a certain interaction between ginseng exosomes and hair follicle stem cell exosomes, therefore, outside this ratio range, the efficacy of the exosome complex will not increase with the increase in the mass of ginseng exosomes or hair follicle stem cell exosomes.

[0033] Preferably, the concentration of the hair follicle stem cell exosomes is 3E+10 to 1E+11 particle / mL.

[0034] Preferably, the concentration of the ginseng exosomes is greater than 3E+10particle / mL.

[0035] The applicant used dihydrotestosterone to establish an androgenic alopecia model on hair papilla cells, added hair follicle stem cell exosomes and ginseng exosomes, and verified the regulation of hair follicle stem cell-derived exosomes and ginseng-derived exosomes on the proliferation activity of hair papilla cells by detecting the expression of hair papilla cell proliferation-related factors and growth factor VEGF.

[0036] The verification method specifically includes the following steps:

[0037] The dermal papilla cells were cultured, and DHT (dihydrotestosterone) was added to construct an androgen model. The corresponding exosomes were added and treated according to the concentration gradient, and the cell viability was detected after 48 hours.

[0038] 1) Human dermal papilla cells were cultured and the cell concentration was adjusted to 0.8×10 4 cells / mL, inoculated into 96-well culture plates, 100 μL per well, and cultured in a 37°C, 5% CO2 incubator for 24 h;

[0039] 2) Discard the supernatant and divide the male baldness model group into experimental groups. The male baldness model group was induced with 100 μM DHT for 24 h, and 5 replicate wells were set for each group.

[0040] 3) After 24 hours, the supernatant was discarded and the hair follicle stem cell exosomes were diluted to different concentrations, concentration 1: 3E+9particle / mL, concentration 2: 1E+10particle / mL, concentration 3: 3E+10particle / mL, concentration 4: 1E+11particle / mL. According to the experimental groups, 10% volume of hair follicle stem cell exosomes were added to the corresponding cells for 48 hours to detect cell viability. The statistical results are shown in Figure 1-Figure 3 ,in Figure 1 This is a comparison of the effects of different concentrations of hair follicle stem cell exosomes on the proliferation activity of hair papilla cells. Figure 2 This is a comparison chart of the regulatory effects of different concentrations of hair follicle stem cell exosomes on the growth factor VEGF. Figure 3 The figure shows the comparison of the regulatory effects of different concentrations of hair follicle stem cell exosomes on the Wnt / β-catenin signaling pathway. It can be seen from the comparison figures that the treatment of 3E+10particle / mL hair follicle stem cell exosomes at a concentration of 3 can significantly improve the cell viability after model damage, and the growth factor VEGF and Wnt / β-catenin signaling pathway are also regulated, but the hair follicle stem cell exosome treatment concentration is further increased to 1E+11particle / mL, and there is no significant difference in the proliferation ability of hair papilla cells and the regulation of growth factors such as VEGF. This result shows that hair follicle stem cell exosomes can effectively promote the proliferation of hair papilla cells and induce hair regeneration in the concentration range of 3E+10 to 1E+11particle / mL.

[0041] Similarly, ginseng exosomes were diluted to different concentrations, concentration 1: 1E+10particle / mL, concentration 2: 3E+10particle / mL, concentration 3: 1E+11particle / mL, concentration 4: 3E+11particle / mL. After the above steps 1) and 2), different concentrations of ginseng exosomes were used for treatment. After treatment, the IL-6 level in the cells decreased, and as the treatment concentration of ginseng exosomes increased, the inhibitory effect on inflammatory factors became stronger and dose-dependent. Statistical results are shown in Figure 4 and Figure 5 , Figure 4 This is a comparison of the inhibitory effects of ginseng exosomes at different concentrations on IL-6 inflammatory factors. Figure 5 This is a comparison chart of the regulatory effects of ginseng exosomes at different concentrations on the Wnt / β-catenin signaling pathway. From the comparison of the drawings, it can be seen that ginseng exosomes can effectively inhibit inflammation and regulate the Wnt / β-catenin signaling pathway, and that exosome concentrations greater than 3E+10particle / mL can have significant anti-inflammatory effects and signaling pathway regulatory effects.

[0042] Preferably, the exosome complex for hair growth provided by the present invention also includes a polypeptide composition, and the exosome composition is 20-35 parts by mass, and the polypeptide composition is 8-15 parts. Through the combined application of the exosome composition and the polypeptide composition, the growth cycle and scalp microenvironment of hair follicle stem cells, hair papilla cells and hair follicles can be regulated, providing a suitable liquid environment for the exosomes to play a role, and improving the hair growth effect. Too little addition of the polypeptide composition often fails to achieve the corresponding effect, and excessive addition of the polypeptide composition will affect the activity of the hair papilla cells, thereby affecting the growth of the hair follicles.

[0043] In the present invention, the peptide composition uses active hair growth peptides at a concentration of 10-2000ppm, including a combination of one or more of copper peptides, acetyl tetrapeptide-3, and oligopeptide-54. Copper peptides can promote the proliferation of hair papilla cells, increase the stemness and proliferation potential of hair follicle stem cells, and promote the production of vascular endothelial factor (VEGF) to improve the scalp microenvironment. Acetyl tetrapeptide-3 can increase the synthesis of extracellular matrix such as collagen IV and laminin, or directly act on the tissues around the hair follicles, delay hair follicle aging, improve hair follicle structure, and stimulate hair growth. Oligopeptide-54 inhibits hair loss by downregulating DKK-1, BMP4 and TGF-β1 signals, while stimulating hair follicles and promoting hair growth.

[0044] The exosome composition and the polypeptide composition work together to exert their effects in different cells and at different times. Since the polypeptide has a small molecular weight and is quickly absorbed by cells, the polypeptide composition regulates the activity and proliferation of hair papilla and hair follicle stem cells in the early stage, activates the signal of hair follicle stem cells, and promotes hair growth. However, due to the recognition characteristics of the membrane structure and surface specific proteins of the exosome composition, the active ingredients can be more accurately delivered to damaged or dormant hair follicle stem cells. After membrane fusion or phagocytosis, the nucleic acid and protein biomacromolecules in the exosomes play a role in regulating hair growth in the later stage of the action.

[0045] It should be noted that the exosome complex provided by the present invention can be administered via a route suitable for the condition to be treated, including external application or subcutaneous injection.

[0046] The present invention is further described in detail with reference to the following examples. In the following examples and comparative examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified, and the consumables used are all subjected to endotoxin removal and high-pressure wet heat sterilization.

[0047] In the following embodiments and comparative examples, the method for preparing the exosome complex is to first prepare an exosome composition, purify the plant exosomes and / or human stem cell exosomes to the corresponding concentration according to the existing method, and prepare them as mixed lyophilized powders, dissolve the mixed lyophilized powders into the corresponding number of polypeptide compositions in a complex including a polypeptide composition, and stir until the system is uniform.

[0048] Example 1

[0049] The exosome complex for hair growth provided in this embodiment includes: an exosome composition, the exosome composition further includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 8 parts and the hair follicle stem cell exosomes are 16 parts by weight.

[0050] Example 2

[0051] The exosome complex for hair growth provided in this embodiment includes: an exosome composition, the exosome composition further includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 6 parts and the hair follicle stem cell exosomes are 18 parts by weight.

[0052] Example 3

[0053] The exosome complex for hair growth provided in this embodiment includes: an exosome composition, the exosome composition further includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 9.6 parts and the hair follicle stem cell exosomes are 14.4 parts by weight.

[0054] Example 4

[0055] The exosome complex for hair growth provided in this embodiment includes: an exosome composition and a polypeptide composition, the exosome composition further includes ginseng exosomes at a concentration of 3E+10particle / mL and hair follicle stem cell exosomes at a concentration of 3E+10particle / mL, and the polypeptide composition includes copper peptides at a concentration of 1000ppm, 500ppm acetyl tetrapeptide-3, and 1000ppm oligopeptide-54. In terms of mass fractions, the ginseng exosomes are 8 parts, the hair follicle stem cell exosomes are 16 parts, and the copper peptides, acetyl tetrapeptide-3, and oligopeptide-54 are 4 parts respectively.

[0056] Comparative Example 1

[0057] Calculated by weight, the exosome complex for hair growth provided in this comparative example includes: 24 portions of ginseng exosomes with a concentration of 3E+10particle / mL.

[0058] Comparative Example 2

[0059] Calculated by weight, the exosome complex for hair growth provided in this comparative example includes: 24 parts of hair follicle stem cell exosomes with a concentration of 3E+10particle / mL.

[0060] Comparative Example 3

[0061] The exosome complex for hair growth provided in this comparative example includes: an exosome composition, wherein the exosome composition includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 4.8 parts and the hair follicle stem cell exosomes are 19.2 parts by weight.

[0062] Comparative Example 4

[0063] The exosome complex for hair growth provided in this comparative example includes: an exosome composition, wherein the exosome composition includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 8 parts and the hair follicle stem cell exosomes are 28 parts by weight.

[0064] Comparative Example 5

[0065] The exosome complex for hair growth provided in this comparative example includes: an exosome composition, wherein the exosome composition includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 8 parts and the hair follicle stem cell exosomes are 10 parts by weight.

[0066] Comparative Example 6

[0067] The exosome complex for hair growth provided in this comparative example includes: an exosome composition, the exosome composition includes ginseng exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginseng exosomes are 12 parts and the hair follicle stem cell exosomes are 12 parts by weight.

[0068] Comparative Example 7

[0069] The exosome complex for hair growth provided in this comparative example includes: a polypeptide composition, which includes 4 parts of copper peptide with a concentration of 1000 ppm, 500 ppm acetyl tetrapeptide-3, and 1000 ppm oligopeptide-54, calculated by weight.

[0070] Comparative Example 8 (replaced with ginger exosomes)

[0071] In this comparative example, ginseng exosomes are replaced with ginger exosomes, and the exosome complex for hair growth provided includes: an exosome composition, the exosome composition includes ginger exosomes with a concentration of 3E+10particle / mL and hair follicle stem cell exosomes with a concentration of 3E+10particle / mL, and the ginger exosomes are 8 parts and the hair follicle stem cell exosomes are 16 parts by weight.

[0072] The formulas of the exosome complexes for hair growth provided in Examples 1 to 4 and Comparative Examples 1 to 8 are shown in Table 1:

[0073] Table 1

[0074]

[0075]

[0076] The present invention uses DHT (dihydrotestosterone) to establish an androgenic alopecia model on hair papilla cells, adds the exosome complexes of each embodiment and comparative example, and determines the efficacy of different formulas on hair papilla cell growth by detecting the proliferation activity of hair papilla cells. The test results are as follows: Figure 6 shown.

[0077] It can be seen from the test results that after the exosome complexes provided in each embodiment and comparative example acted on the male pattern baldness model, the proliferation activity of the dermal papilla cells was better than that of the original male pattern baldness model group.

[0078] The exosome complexes provided in Examples 1 to 4 have overall effects that are better than or slightly equal to those of the comparative examples, and the exosome complex provided in Example 4, which is a compound of an exosome composition and a polypeptide composition, has the best effect. It can be seen that the use of the composite polypeptide composition in combination with the ginseng exosome and hair follicle stem cell exosome ratio provided by the present invention can significantly enhance the proliferation activity of hair papilla cells.

[0079] Comparative Examples 1 and 2 respectively used a single combination of ginseng exosomes and hair follicle stem cell exosomes, and their effects were not as good as those of the examples, indicating that ginseng exosomes and hair follicle stem cell exosomes have a certain mutual promotion effect, and the effect achieved by their combination is better. The effect of Comparative Example 2 is slightly better than that of Comparative Example 1, indicating that the effect of hair follicle stem cell exosomes on the proliferation activity of hair papilla cells is slightly better than that of ginseng exosomes.

[0080] In Comparative Examples 3 and 4, the content of hair follicle stem cell exosomes is slightly higher, and the mass ratio of ginseng exosomes to hair follicle stem cell exosomes is about 1: (3.5-4), and the effect is slightly lower than that of Example 1 or is basically the same as that of Example 1, indicating that outside the ratio set by the present invention, the effect of the exosome complex will not increase with the increase of hair follicle stem cell exosomes.

[0081] In Comparative Examples 5 and 6, the content of ginseng exosomes is slightly higher, and the mass ratio of ginseng exosomes to hair follicle stem cell exosomes is about 1: (1-1.25), and the effect is lower than that in Example 1, indicating that if the content of hair follicle stem cell exosomes, which play a major role, is too low, it will affect the expression of the overall effect of the exosome complex.

[0082] In Comparative Example 7, only the polypeptide composition was used, and its effect on promoting the proliferation activity of hair papilla cells was significantly worse than that of the combined combination of the exosome composition and the polypeptide composition provided in Example 4.

[0083] In Comparative Example 8, ginseng exosomes were replaced with ginger exosomes, and their promoting effect on the proliferation activity of hair papilla cells was significantly worse than the combination of ginseng exosomes and hair follicle stem cell exosomes provided in Example 1, indicating that ginseng exosomes can promote the proliferation and differentiation of hair papilla cells, and other plant exosomes such as non-ginger exosomes can be used as a substitute.

[0084] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An exosome complex for hair growth, characterized in that: The invention comprises an exosome composition, wherein the exosome composition comprises hair follicle stem cell exosomes and ginseng exosomes.

2. The exosome complex for hair growth according to claim 1, characterized in that: In the exosome composition, the mass ratio of the ginseng exosomes to the hair follicle stem cell exosomes is 1:(1.5-3).

3. The exosome complex for hair growth according to claim 2, characterized in that: The mass ratio of the ginseng exosomes to the hair follicle stem cell exosomes is 1:(2-3).

4. The exosome complex for hair growth according to claim 1, characterized in that: The concentration range of the hair follicle stem cell exosomes is 3E+10 to 1E+11 particle / mL.

5. The exosome complex for hair growth according to claim 1, characterized in that: The concentration of the ginseng exosomes is greater than 3E+10particle / mL.

6. The exosome complex for hair growth according to claim 1, characterized in that The exosome complex also includes a polypeptide composition. In terms of weight percentage, the exosome composition is 20-35 parts, and the polypeptide composition is 8-15 parts.

7. The exosome complex for hair growth according to claim 6, characterized in that: The polypeptide composition comprises: a combination of one or more of copper peptide, acetyl tetrapeptide-3 and oligopeptide-54.

8. A hair growth liquid containing the exosome complex for hair growth according to any one of claims 1 to 7.

9. The hair growth liquid according to claim 8, characterized in that: The content of the exosome complex in the hair growth fluid is 3-15wt%.

10. Use of the exosome complex according to any one of claims 1 to 7 or the hair growth liquid according to any one of claims 8 or 9 in hair growth, hair growth, and hair loss prevention products.

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