A medicated pack for local edema after tumor surgery and radiotherapy and its preparation method

Abstract

This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a medicated pack for local edema after tumor surgery and radiotherapy, and its preparation method. The medicated pack comprises the following raw materials in parts by weight: Astragalus membranaceus 20-30 parts, Paeonia lactiflora 20-30 parts, Glauber's salt 20-30 parts, Poria cocos 15-20 parts, Curcuma longa 15-20 parts, Borneol 10-15 parts, Typha pollen 10-15 parts, self-heating matrix 20-40 parts, hyaluronic acid 30-70 parts, and glycerin 50-80 parts. The traditional Chinese medicines used have the effects of promoting diuresis and reducing swelling, promoting blood circulation and removing blood stasis, and anti-inflammatory and analgesic properties. The prepared self-heating matrix has the characteristics of continuous and controllable hygroscopic and heat-releasing properties, which can promote the formation of a gel state in the medicated pack during use, providing a warm environment for the transdermal transdermal absorption of the active ingredients of the traditional Chinese medicine, effectively improving the symptoms of local edema after tumor surgery and radiotherapy, and has high clinical application value.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a medicated pack for local edema after tumor surgery and radiotherapy and its preparation method. Background Technology

[0002] Postoperative and radiotherapy treatments for tumors often result in localized edema, pain, and slow wound healing. This localized edema not only affects the speed of recovery but can also lead to complications such as poor blood circulation, tissue hypoxia, and persistent inflammation. Furthermore, tumor radiotherapy increases local skin sensitivity, making wounds more susceptible to infection and irritation, further complicating treatment. While traditional physical cooling methods, pressure bandages, and conventional topical ointments can alleviate edema and pain to some extent, they have many limitations. For example, ordinary topical medications require additional heating or cooling equipment, which is inconvenient, and their moisturizing and antibacterial effects are weak, making them unsuitable for prolonged use. Some topical medications may also trigger skin sensitivity or allergic reactions due to their highly irritating ingredients, especially after tumor surgery or radiotherapy when the skin is vulnerable and the patient's resistance is lowered, making them more susceptible to pathogenic microorganisms.

[0003] Currently, topical products available on the market for patients after tumor surgery and radiotherapy have limited functions. There is an urgent need for a convenient and multifunctional anti-edema pack to provide cancer patients with a more efficient solution for treating local edema. Summary of the Invention

[0004] In response to the above situation, the present invention provides a local edema pack and its preparation method after tumor surgery and radiotherapy. It uses active substances of traditional Chinese medicine as the main components for reducing swelling, antibacterial and analgesic effects. It has the characteristics of being safe and mild. Magnesium sulfate and calcium chloride are encapsulated in a hygroscopic gel composite matrix. When exposed to air, the matrix absorbs moisture and releases heat, providing suitable transdermal conditions for the traditional Chinese medicine components and relieving edema after tumor surgery or radiotherapy.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] This invention provides a medicated pack for local edema after tumor surgery and radiotherapy. The medicated pack comprises the following raw materials in parts by weight: 20-30 parts Astragalus membranaceus, 20-30 parts Paeonia lactiflora, 20-30 parts Glauber's salt, 15-20 parts Poria cocos, 15-20 parts Curcuma longa, 10-15 parts Borneol, 10-15 parts Typha pollen, 20-40 parts self-heating matrix, 30-70 parts Hyaluronic acid, and 50-80 parts Glycerin.

[0007] Furthermore, the self-heating matrix comprises the following raw materials in parts by weight: 50-80 parts magnesium sulfate, 30-50 parts calcium chloride, and 20-50 parts chitosan.

[0008] Furthermore, the degree of deacetylation of the chitosan is 85-95%, and the molecular weight is 100-150 kDa.

[0009] The preparation method of the self-heating substrate includes the following steps:

[0010] S1: Weigh 20-50 parts of chitosan and dissolve it in pH 5.0 acetate buffer at a mass-to-volume ratio of 1 g: 6 mL to obtain a chitosan solution;

[0011] S2: Weigh 50-80 parts of magnesium sulfate and 30-50 parts of calcium chloride, mix them to obtain a mixture, and dissolve the mixture in purified water under ice bath conditions at a mass-volume ratio of 1 g: 4 mL to obtain a mixture solution;

[0012] S3: Add the mixture solution obtained in step S2 to the chitosan solution obtained in step S1, and stir for 20 min under magnetic stirring at 100 rpm to obtain a self-heating matrix solution.

[0013] S4: Spray dry the self-heating matrix solution obtained in step S3. The inlet temperature of the spray dryer is 150-200℃, the outlet temperature is 60-100℃, the atomizer speed is 21500 rpm, and the electric heating power is 60 kW to obtain the self-heating matrix.

[0014] This invention also provides a method for preparing a medicated pack for local edema after tumor surgery and radiotherapy, specifically including the following steps:

[0015] Step 1: Weigh out 20-30 parts of Astragalus membranaceus, 20-30 parts of Paeonia lactiflora, 20-30 parts of Glauber's salt, 15-20 parts of Poria cocos, 15-20 parts of Curcuma longa, 10-15 parts of Borneol and 10-15 parts of Typha pollen, mix them, grind them into powder, and pass them through a 120-mesh sieve to obtain Chinese herbal powder.

[0016] Step 2: Weigh 30-70 parts of hyaluronic acid and 50-80 parts of glycerin, add them to the Chinese herbal powder obtained in Step 1, stir at 200 rpm for 30 min to obtain a viscous Chinese herbal matrix;

[0017] Step 3: Dry the Chinese herbal matrix obtained in Step 2 in an oven at 60℃ for 12 hours to obtain Chinese herbal matrix powder;

[0018] Step 4: Weigh 20-40 parts of the self-heating matrix, mix it evenly with the Chinese medicine matrix powder obtained in Step 3, and vacuum seal it to obtain a local edema medicine pack after tumor surgery and radiotherapy.

[0019] The beneficial effects achieved by this invention are as follows:

[0020] The local edema medication pack prepared by this invention after tumor surgery and radiotherapy uses traditional Chinese medicine as the main active ingredients to exert its effects transdermally. The paeoniflorin, saponins, and curcumin in the red peony root, mirabilite, turmeric, and borneol have the effects of promoting blood circulation, removing blood stasis, anti-inflammatory and analgesic properties. The polysaccharides and volatile oils in astragalus root, poria cocos, and cattail pollen have the effects of promoting diuresis, reducing swelling, strengthening the spleen and regulating menstruation. The magnesium sulfate in the self-heating matrix, when exposed to air, has strong hygroscopic properties, which can promote the formation of a gel state by chitosan, hyaluronic acid, and glycerin. Simultaneously, the hygroscopic process of magnesium sulfate generates heat. Furthermore, it reacts with the polyphenolic components of traditional Chinese medicine to generate heat, providing a warm environment for the transdermal absorption of the medicinal components without the need for an additional heater. Calcium chloride and chitosan can control the exothermic temperature and duration of magnesium sulfate, and effectively encapsulate magnesium sulfate in the formed gel without contacting the skin and causing irritation, thus having a long-lasting heat-generating effect. In addition, hyaluronic acid and glycerin also have moisturizing effects, preventing the skin from drying and dehydrating due to prolonged dressing. Chitosan and other traditional Chinese medicine components such as curcumin and saponins also have antibacterial activity, effectively preventing local infections after tumor surgery and radiotherapy.

[0021] The local edema pack provided by this invention after tumor surgery and radiotherapy uses a moisture-absorbing and self-heating mechanism and utilizes the active ingredients of traditional Chinese medicine for treatment. It is characterized by simple operation, safety and long-lasting effect, and the ability to reduce swelling and relieve pain, and has high clinical application value. Attached Figure Description

[0022] Figure 1 This is a flowchart illustrating the preparation process of the edema medication pack for post-tumor surgery and post-radiotherapy according to the present invention.

[0023] Figure 2 The results of the self-heating temperature change over time for the edema packs prepared after tumor surgery and radiotherapy in Comparative Example 1 and Examples 1-3.

[0024] Figure 3 The results show the net weight of granulomas and the rate of inflammation suppression in rats after tumor surgery and radiotherapy using the edema packs prepared in Examples 1-3 and Comparative Examples 1-3.

[0025] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof. Detailed Implementation

[0026] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those familiar to those skilled in the art. Furthermore, any methods and materials similar to or equivalent to those described herein may be applied to this invention. The preferred embodiments and materials described herein are for illustrative purposes only and do not limit the scope of this application.

[0028] In the following embodiments, the preparation process of the local edema medication pack after tumor surgery and radiotherapy is as follows: Figure 1 Unless otherwise specified, all methods are conventional. Unless otherwise specified, all materials used in the following examples are new materials purchased from the market.

[0029] Example 1: This example provides a local edema medication pack after tumor surgery and radiotherapy. The medication pack includes the following raw materials in parts by weight: 20 parts Astragalus membranaceus, 20 parts Paeonia lactiflora, 20 parts Glauber's salt, 15 parts Poria cocos, 15 parts Curcuma longa, 10 parts Borneol, 10 parts Typha pollen, 20 parts self-heating matrix, 30 parts Hyaluronic acid, and 50 parts Glycerin.

[0030] The self-heating matrix comprises the following raw materials in parts by weight: 50 parts magnesium sulfate, 50 parts calcium chloride, and 30 parts chitosan. The specific preparation method is as follows:

[0031] S1: Weigh 30 parts of chitosan and dissolve them in pH 5.0 acetate buffer at a mass-to-volume ratio of 1 g: 6 mL to obtain a chitosan solution;

[0032] S2: Weigh 50 parts of magnesium sulfate and 50 parts of calcium chloride, mix them to obtain a mixture, and dissolve the mixture in purified water under ice bath conditions at a mass-volume ratio of 1 g: 4 mL to obtain a mixture solution;

[0033] S3: Add the mixture solution obtained in step S2 to the chitosan solution obtained in step S1, and stir under magnetic stirring at 100 rpm for 20 min to obtain a self-heating matrix solution;

[0034] S4: The self-heating matrix solution obtained in step S3 is spray-dried. The spray dryer has an inlet temperature of 150°C, an outlet temperature of 60°C, an atomizer speed of 21,500 rpm, and an electric heating power of 60 kW to obtain the self-heating matrix.

[0035] This embodiment also provides a method for preparing a local edema medication pack after tumor surgery and radiotherapy, specifically including the following steps:

[0036] Step 1: Weigh out 20 parts of Astragalus membranaceus, 20 parts of Paeonia lactiflora, 20 parts of Glauber's salt, 15 parts of Poria cocos, 15 parts of Curcuma longa, 10 parts of Borneol and 10 parts of Typha pollen, mix them, grind them into powder, and pass them through a 120-mesh sieve to obtain Chinese herbal powder.

[0037] Step 2: Weigh 30 parts of hyaluronic acid and 50 parts of glycerin, add them to the Chinese herbal powder obtained in Step 1, stir at 200 rpm for 30 min to obtain a viscous Chinese herbal matrix;

[0038] Step 3: Dry the Chinese herbal matrix obtained in Step 2 in an oven at 60℃ for 12 hours to obtain Chinese herbal matrix powder;

[0039] Step 4: Weigh 20 parts of the self-heating matrix, mix them evenly with the Chinese medicine matrix powder obtained in Step 3, and vacuum seal them to obtain a local edema medicine pack after tumor surgery and radiotherapy.

[0040] Example 2: This example provides a local edema medication pack after tumor surgery and radiotherapy. The medication pack includes the following raw materials in parts by weight: 25 parts Astragalus membranaceus, 25 parts Paeonia lactiflora, 25 parts Glauber's salt, 20 parts Poria cocos, 20 parts Curcuma longa, 15 parts Borneol, 15 parts Typha pollen, 40 parts self-heating matrix, 70 parts Hyaluronic acid, and 80 parts Glycerin.

[0041] The self-heating matrix comprises the following raw materials in parts by weight: 70 parts magnesium sulfate, 40 parts calcium chloride, and 50 parts chitosan. The specific preparation method is as follows:

[0042] S1: Weigh 50 parts of chitosan and dissolve them in pH 5.0 acetate buffer at a mass-to-volume ratio of 1 g: 6 mL to obtain a chitosan solution;

[0043] S2: Weigh 70 parts of magnesium sulfate and 40 parts of calcium chloride, mix them to obtain a mixture, and dissolve the mixture in purified water under ice bath conditions at a mass-volume ratio of 1 g: 4 mL to obtain a mixture solution;

[0044] S3: Add the mixture solution obtained in step S2 to the chitosan solution obtained in step S1, and stir for 20 min under magnetic stirring at 100 rpm to obtain a self-heating matrix solution.

[0045] S4: The self-heating matrix solution obtained in step S3 is spray-dried. The spray dryer has an inlet temperature of 150°C, an outlet temperature of 60°C, an atomizer speed of 21,500 rpm, and an electric heating power of 60 kW to obtain the self-heating matrix.

[0046] This embodiment also provides a method for preparing a local edema medication pack after tumor surgery and radiotherapy, which specifically includes the following steps:

[0047] Step 1: Weigh out 25 parts of Astragalus membranaceus, 25 parts of Paeonia lactiflora, 25 parts of Glauber's salt, 20 parts of Poria cocos, 20 parts of Curcuma longa, 15 parts of Borneol and 15 parts of Typha pollen, mix them, grind them into powder, and pass them through a 120-mesh sieve to obtain Chinese herbal powder.

[0048] Step 2: Weigh 70 parts of hyaluronic acid and 80 parts of glycerin, add them to the Chinese herbal powder obtained in Step 1, stir at 200 rpm for 30 min to obtain a viscous Chinese herbal matrix;

[0049] Step 3: Dry the Chinese herbal matrix obtained in Step 2 in an oven at 60℃ for 12 hours to obtain Chinese herbal matrix powder;

[0050] Step 4: Weigh 40 parts of the heat-generating matrix, mix it evenly with the Chinese medicine matrix powder obtained in Step 3, and vacuum seal it to obtain a local edema medication pack after tumor surgery and radiotherapy.

[0051] Example 3: This example provides a local edema medication pack after tumor surgery and radiotherapy. The medication pack includes the following raw materials in parts by weight: 30 parts Astragalus membranaceus, 30 parts Paeonia lactiflora, 30 parts Glauber's salt, 18 parts Poria cocos, 18 parts Curcuma longa, 12 parts Borneol, 12 parts Typha pollen, 30 parts self-heating matrix, 50 parts Hyaluronic acid, and 60 parts Glycerin.

[0052] The self-heating matrix comprises the following raw materials in parts by weight: 80 parts magnesium sulfate, 30 parts calcium chloride, and 20 parts chitosan. The specific preparation method is as follows:

[0053] S1: Weigh 20 parts of chitosan and dissolve them in pH 5.0 acetate buffer at a mass-to-volume ratio of 1 g: 6 mL to obtain a chitosan solution;

[0054] S2: Weigh 80 parts of magnesium sulfate and 30 parts of calcium chloride, mix them to obtain a mixture, and dissolve the mixture in purified water under ice bath conditions at a mass-volume ratio of 1 g: 4 mL to obtain a mixture solution;

[0055] S3: Add the mixture solution obtained in step S2 to the chitosan solution obtained in step S1, and stir for 20 min under magnetic stirring at 100 rpm to obtain a self-heating matrix solution.

[0056] S4: The self-heating matrix solution obtained in step S3 is spray-dried. The spray dryer has an inlet temperature of 150°C, an outlet temperature of 60°C, an atomizer speed of 21,500 rpm, and an electric heating power of 60 kW to obtain the self-heating matrix.

[0057] This embodiment also provides a method for preparing a local edema medication pack after tumor surgery and radiotherapy, which specifically includes the following steps:

[0058] Step 1: Weigh out 30 parts of Astragalus membranaceus, 30 parts of Paeonia lactiflora, 30 parts of Glauber's salt, 18 parts of Poria cocos, 18 parts of Curcuma longa, 12 parts of Borneol and 12 parts of Typha pollen, mix them, grind them into powder, and pass them through a 120-mesh sieve to obtain Chinese herbal powder.

[0059] Step 2: Weigh 50 parts of hyaluronic acid and 60 parts of glycerin, add them to the Chinese herbal powder obtained in Step 1, stir at 200 rpm for 30 min to obtain a viscous Chinese herbal matrix;

[0060] Step 3: Dry the Chinese herbal matrix obtained in Step 2 in an oven at 60℃ for 12 hours to obtain Chinese herbal matrix powder;

[0061] Step 4: Weigh 30 parts of the self-heating matrix, mix it evenly with the Chinese medicine matrix powder obtained in Step 3, and vacuum seal it to obtain a local edema medicine pack after tumor surgery and radiotherapy.

[0062] The difference between Comparative Example 1 and Example 1 is that magnesium sulfate and calcium chloride were not added; the rest of the parts are the same as in Example 1.

[0063] The difference between Comparative Example 2 and Example 1 is that chitosan was not added; the rest of the parts are the same as in Example 1.

[0064] The difference between Comparative Example 3 and Example 1 is that glycerol was not added; the rest of the parts are the same as in Example 1.

[0065] Examination of the change of self-heating temperature over time

[0066] Take the medicine packets prepared in Comparative Example 1 and Examples 1-3, open the packaging, and use a temperature gun to measure the temperature of the medicine packets at time points of 10 s, 1 min, 20 min, 1 h, 2 h, and 4 h. The results are shown in the figure. Figure 2 .

[0067] Inflammation suppression rate assessment

[0068] SPF-grade SD rats weighing 200-220 g were selected. Hair was removed from both groins and abdomen. The rats were anesthetized with isoflurane. In a sterile environment, 20 mg sterile dry cotton balls were subcutaneously inserted into both groins. The incisions were then sutured. The rats with only an incision and subsequent suturing were designated as the sham surgery group. The control group (no drug administration), the group using the drug packs prepared in Examples 1-3, and the comparative examples 1-3, and the comparative examples 1-3, respectively, served as the comparative examples 1, 2, and 3 groups. Three rats were observed in each group. The drug was applied for 2 hours each time, twice a day, with a 6-hour interval between applications, for 7 consecutive days. After the last administration, the rats were anesthetized and euthanized. Granulomas from the groin area were collected, weighed (wet weight), dried at 60℃ for 24 hours (dry weight), and the net weight of the granulomas and the inflammation inhibition rate were calculated. The results are shown in [Figure number missing]. Figure 3 Granuloma net weight = granuloma weight after drying cotton ball - original cotton ball weight; Inflammation inhibition rate % = (average net weight of granuloma in control group - average net weight of granuloma in treatment group) / average net weight of granuloma in control group × 100%.

[0069] Treatment effect

[0070] One hundred and fourteen patients with postoperative or post-radiotherapy edema were randomly divided into a control group and an observation group, with 52 patients in each group. The mean age of the control group was 42.25 ± 8.23 ​​years and the mean duration of disease was 5.02 ± 1.12 months. The mean age of the observation group was 44.12 ± 8.10 years and the mean duration of disease was 5.18 ± 1.08 months. Patients in the control group received routine oral diosmin tablets, 0.9 g / time, twice a day, and lymphatic drainage treatment. Patients in the observation group received the drug pack prepared in Example 1, twice a day, every 2 hours, with an interval of 6 hours between each dose. h. Lymphatic drainage therapy; all groups were treated for 3 weeks. The efficacy criteria were as follows: Cured: no recurrence of upper limb edema, pain, and other symptoms, and a reduction of ≥75% in the circumference difference between the affected and healthy sides of the upper limb; Significantly effective: significant improvement in upper limb edema and pain symptoms, 75% > a reduction of ≥50% in the circumference difference between the affected and healthy sides of the upper limb; Effective: some relief in upper limb edema and pain symptoms, 50% > a reduction of ≥25% in the circumference difference between the affected and healthy sides of the upper limb; Ineffective: no relief or even worsening of the above symptoms, and a reduction of <25% in the circumference difference between the affected and healthy sides of the upper limb. The efficacy results are shown in Table 1. The occurrence of adverse reactions in both groups was also recorded, and the results are shown in Table 2.

[0071] Table 1 Comparison of clinical efficacy between the two groups of patients

[0072]

[0073] Table 2 Comparison of adverse reaction occurrences between the two groups of patients

[0074]

[0075] Figure 2 The results showed that the prepared self-heating matrix had good self-heating properties and could maintain a temperature of about 40°C for 3 hours, which could greatly improve the efficiency of clinical hot compress.

[0076] Figure 3 The results showed that Examples 1-3 and Comparative Examples 1-3 all had good inflammation suppression rates, and the traditional Chinese medicines used had good anti-inflammatory effects.

[0077] The clinical treatment results in Tables 1 and 2 show that the medicine pack prepared in Example 1 is effective in relieving edema after tumor surgery or radiotherapy, and is safe to use with low adverse reactions and high patient compliance.

[0078] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

[0079] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention. The actual application is not limited to this. In conclusion, if those skilled in the art are inspired by this description and design similar methods and embodiments without departing from the spirit of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A medication pack for local edema after tumor surgery and radiotherapy, characterized in that, The medicine package is composed of the following raw materials in parts by weight: Astragalus membranaceus 20-30 parts, Paeonia lactiflora 20-30 parts, Glauber's salt 20-30 parts, Poria cocos 15-20 parts, Curcuma longa 15-20 parts, Borneol 10-15 parts, Typha pollen 10-15 parts, self-heating matrix 20-40 parts, hyaluronic acid 30-70 parts, and glycerin 50-80 parts; The self-heating matrix comprises the following raw materials in parts by weight: 50-80 parts magnesium sulfate, 30-50 parts calcium chloride, and 20-50 parts chitosan. The specific preparation method is as follows: S1: Weigh out chitosan and dissolve it in acetate buffer to obtain a chitosan solution; S2: Weigh out magnesium sulfate and calcium chloride, mix them, and dissolve them in purified water to obtain a mixed solution; S3: Add the mixture solution obtained in step S2 to the chitosan solution obtained in step S1, stir, and obtain a self-heating matrix solution; S4: Spray dry the self-heating matrix solution obtained in step S3 to obtain the self-heating matrix.

2. The local edema medication pack after tumor surgery and radiotherapy according to claim 1, characterized in that, In step S1, the mass-to-volume ratio of chitosan to acetate buffer is 1 g: 6 mL, and the pH of the acetate buffer is 5.

0. In step S2, the mixture obtained after mixing has a mass-to-volume ratio of 1 g to 4 mL of purified water, and the dissolution process is carried out in an ice bath. In step S4, the spray drying conditions are: inlet temperature 150-200℃, outlet temperature 60-100℃, atomizer speed 21500rpm, and electric heating power 60kW.

3. A local edema medication pack for post-tumor surgery and radiotherapy as described in claim 1, characterized in that, The chitosan has a degree of deacetylation of 85-95% and a molecular weight of 100-150 kDa.

4. A method for preparing a local edema medication pack after tumor surgery and radiotherapy according to any one of claims 1-3, characterized in that, The specific preparation method is as follows: Step 1: Weigh out Astragalus membranaceus, Paeonia lactiflora, Glauber's salt, Poria cocos, Curcuma longa, Borneol and Typha pollen, mix them, grind them into powder, and sieve them to obtain Chinese herbal powder. Step 2: Weigh hyaluronic acid and glycerin, add them to the Chinese herbal powder obtained in Step 1, and stir to obtain a viscous Chinese herbal matrix; Step 3: Dry the Chinese herbal matrix obtained in Step 2 to obtain Chinese herbal matrix powder; Step 4: Weigh the heat-generating matrix and mix it with the Chinese medicine matrix powder obtained in Step 3, then package it to obtain a local edema medication pack after tumor surgery and radiotherapy.

5. The method for preparing a local edema medication pack after tumor surgery and radiotherapy according to claim 4, characterized in that, In step 1, the mesh size of the sieve is 120 mesh; In step 3, the drying process is carried out in an oven at a temperature of 60°C. In step 4, the encapsulation is a vacuum encapsulation.

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