Saffron crocus direct drinking powder as well as preparation method and application thereof

Abstract

The invention provides saffron crocus direct drinking powder as well as a preparation method and application thereof. The saffron crocus direct drinking powder is prepared from the following raw materials: saffron crocus extract, cardiomyopeptide powder, menthol, citric acid, erythritol, maltodextrin and cranberry powder. The saffron crocus direct drinking powder disclosed by the invention has excellent free radical scavenging capacity and myocardial protection function, so that the problems in the aspect of heart and cerebral vessels of a human body are solved, a brand-new way can be provided for treatment of cardiovascular diseases, and the occurrence risk of adverse reactions can be reduced while the treatment effect is improved. In addition, the product also has a good taste and is easily accepted by people.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and in particular to a saffron direct drinking powder. At the same time, the present invention also relates to a preparation method and application of the saffron direct drinking powder. Background Art

[0002] Among the many heart diseases that pose a serious threat to human health, the situation of coronary heart disease is particularly severe, the number of patients continues to rise and the treatment situation remains bleak. Although the main treatments for coronary heart disease, such as intracoronary thrombolysis and coronary artery bypass grafting, have alleviated the condition to a certain extent, the serious damage to the myocardial structure caused by myocardial ischemia and reperfusion has not been effectively solved. The continuous expansion of the scope of necrosis has always been like a haze, covering the prognosis and recovery of patients with coronary heart disease, greatly affecting their quality of life and health.

[0003] Although there are various chemical drugs for the treatment of coronary heart disease on the market, these drugs are often inevitably accompanied by many adverse reactions. For example, some drugs may cause varying degrees of damage to important organs such as the liver and kidneys, leading to abnormal liver and kidney function indicators; some drugs may cause gastrointestinal discomfort, such as nausea, vomiting, diarrhea and other symptoms, which not only brings additional physical pain to patients, but also reduces patients' medication compliance to a certain extent, making the majority of patients more urgent in their demand for better quality therapeutic drugs. In this context, actively screening a product that is both highly effective and low-toxic has become a top priority. Summary of the invention

[0004] In view of this, the present invention aims to provide a saffron direct drinking powder with antioxidant and myocardial protection effects.

[0005] To achieve the above object, the technical solution of the present invention is achieved as follows:

[0006] The invention discloses a saffron direct drinking powder. The raw materials of the saffron direct drinking powder include saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin and cranberry powder.

[0007] Furthermore, the weight portions of each raw material are: 25-30 parts of saffron extract, 20-30 parts of myocardial peptide powder, 0.1-5 parts of menthol, 0.1-5 parts of citric acid, 22-26 parts of erythritol, 10-15 parts of maltodextrin, and 10-15 parts of cranberry powder.

[0008] Saffron is a traditional and precious Chinese medicinal material. The saffron yellow pigment can effectively improve myocardial blood perfusion, reduce myocardial cell damage, and inhibit the expansion of myocardial infarction. Its mechanism of action may involve regulating vascular endothelial function, anti-oxidative stress, and anti-inflammation.

[0009] The saffron direct drinking powder of the present invention, saffron extract as a key component, contains a variety of bioactive substances, which may be an important source of its core efficacy. Myocardial peptide powder is a booster for human heart health, and its small molecule characteristics are conducive to absorption, and can better act on myocardial tissue. Myocardial peptide powder belongs to small molecule peptide substances, which have unique advantages compared to macromolecular proteins. Small molecule peptides can be absorbed by the human gastrointestinal tract more quickly and efficiently, directly enter the blood circulation, and thus play a role faster. In addition, small molecule peptides can target myocardial cells, provide nutritional support for damaged myocardial cells, promote the repair and regeneration of myocardial cells, enhance the contractile function of the myocardium, and further improve the overall functional state of the heart.

[0010] In addition, the antioxidant and anti-inflammatory effects of saffron are conducive to creating a more favorable intracellular environment for myocardial peptide powder to play a nutritional support role. Saffron can remove these free radicals and reduce inflammation, so that myocardial peptide powder can be better absorbed by myocardial cells and more effectively promote the repair process of myocardial cells. At the same time, in the process of enhancing the metabolic function of myocardial cells, myocardial peptide powder is conducive to promoting the metabolism and function of saffron's active ingredients in cells. The two may form a synergistic effect and jointly maintain the health of myocardial cells.

[0011] Cranberry powder is rich in antioxidants, such as proanthocyanidins and flavonoids. Among them, proanthocyanidins have a particularly outstanding antioxidant capacity, which can remove free radicals in the body. Free radicals are unstable molecules that can cause oxidative damage to cells. The antioxidant components in cranberry powder can neutralize free radicals and reduce the oxidative stress of cells, thereby helping to delay cell aging and protect the health of cells in various organs of the body. The flavonoids in cranberry powder can help lower blood cholesterol levels and reduce the oxidation of low-density lipoproteins, thereby reducing the risk of atherosclerosis. Atherosclerosis is the main pathological basis of cardiovascular disease. By reducing this risk, cranberry powder can help maintain cardiovascular health to a certain extent, reduce the incidence of coronary heart disease, myocardial infarction and other diseases, and the vitamin C, vitamin E, flavonoids, etc. it contains can help enhance the body's immunity.

[0012] In addition, menthol brings a refreshing taste and unique flavor, which can add a light sweetness and numbing taste. It can enrich the taste level of saffron direct drinking powder and make the drinking experience more refreshing and pleasant. Citric acid can adjust the pH of the product, optimize the taste and help maintain the stability of the ingredients, preventing the ingredients from chemically changing due to inappropriate pH. Erythritol, as a low-calorie sweetener, meets people's demand for sweetness while taking health into account. It will not decompose due to high temperature or undergo Maillard reaction with others, thereby ensuring that the color, smell and taste of saffron direct drinking powder remain consistent during storage and use. Maltodextrin acts as a filler and thickener to improve the product texture and dissolution performance, so that the direct drinking powder can form a uniform and stable solution after dissolution, avoiding precipitation of ingredients.

[0013] Therefore, the saffron direct drinking powder of the present invention has excellent free radical scavenging ability and protects myocardial function, thereby improving the cardiovascular and cerebrovascular problems of the human body, providing a new way for the treatment of cardiovascular diseases, and reducing the risk of adverse reactions while improving the therapeutic effect. Moreover, it also has a good taste and is easily accepted by people.

[0014] In addition, the present invention also provides a method for preparing the above-mentioned saffron direct drinking powder, comprising the following steps:

[0015] S1. Weighing: weigh saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin, and cranberry powder according to weight;

[0016] S2, granulation: the weighed raw materials are made into wet soft materials to obtain uniform wet granules;

[0017] S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying to obtain dried saffron direct drinking powder;

[0018] S4. Packaging: Divide the dried saffron drinking powder into small packages.

[0019] Furthermore, in step S2, the binder of the wet granules is 95% ethanol.

[0020] Furthermore, in step S2, the particle size of the wet granules is 40-60 mesh.

[0021] Furthermore, in step S3, the water content of the dried saffron drinking powder is no more than 5.0%.

[0022] Further, step S4 includes:

[0023] S41, inner packaging: receive the composite film, adjust the transverse sealing temperature to the preset temperature, and perform inner packaging according to the preset weight of each bag;

[0024] S42. Outer packaging: Carry out outer packaging in accordance with process regulations and inspect according to standards.

[0025] Furthermore, in step S41, the preset weight is 1.2 g / bag, and the weight difference limit requirement is ±7%.

[0026] The preparation method of the saffron direct drinking powder of the present invention uses 95% ethanol as a binder for wet particles to prepare a wet soft material and granulate. Because the volatility of ethanol is moderate, it can effectively bond the raw materials to form wet particles of regular shape and uniform size during the granulation process, which is convenient for subsequent processing and can be quickly volatilized in the subsequent drying step without residual excessive impurities affecting the purity and quality of the product. Moreover, during granulation, the raw materials are fully mixed under the action of the binder, which further ensures that the components are evenly distributed in each particle, avoiding the problem of unstable product efficacy or inconsistent taste caused by local component concentration differences.

[0027] Placing the wet granules in a hot air circulation oven to dry and controlling the moisture content to ≤5.0% can effectively prevent the product from mildew, caking, deterioration, etc., ensure the stability of the product during storage and transportation, and extend the shelf life of the product. At the same time, precise moisture control also helps to maintain the product's solubility, taste and activity of each ingredient, ensuring that the product can achieve the best effect when used. Suitable composite film materials combined with appropriate transverse sealing temperature can provide good barrier properties for the product, effectively block the damage of external factors such as oxygen, moisture, microorganisms, etc. to the product, and maintain the freshness and quality of the product.

[0028] In addition, the present invention also provides the use of the above-mentioned saffron direct drinking powder in preventing or treating cardiovascular diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:

[0030] Figure 1 This is a comparison chart of the hydroxyl radical scavenging rates described in the embodiments of the present invention. DETAILED DESCRIPTION

[0031] The present invention is further described below in conjunction with specific examples, but the present invention is not limited to the following examples. The methods described are conventional methods unless otherwise specified. The raw materials described can be obtained from public commercial channels unless otherwise specified. The percentages described are all mass percentages unless otherwise specified.

[0032] The present embodiment relates to a saffron direct drinking powder, the raw materials of which include saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin, and cranberry powder.

[0033] Among them, saffron extract can be obtained by crushing the saffron raw material, then adding an appropriate amount of organic solvent, and soaking or refluxing extraction under certain temperature and time conditions. Saffron extract mainly contains a variety of carotenoids and their glycoside compounds, among which crocin and crocetin are the most important components. Crocin is a water-soluble terpenoid compound, which is the main pigment component of saffron and gives saffron a unique red color. Crocetin is a hydrolysis product of crocin and has strong lipophilicity. In addition, it also contains safranal, which is the main source of saffron's unique smell. These ingredients play a key role in different pharmacological effects and application scenarios.

[0034] In addition, saffron extract has the function of regulating blood circulation. Crocin can reduce blood viscosity and inhibit platelet aggregation, thereby reducing the risk of thrombosis. At the same time, the carotenoid components in saffron extract have strong antioxidant capacity. They can remove free radicals in the body. Free radicals are harmful substances produced during the human body's metabolism, which can cause cell damage, aging and various diseases. Therefore, saffron extract can protect cells from free radicals through antioxidant effects, thereby delaying cell aging, and may also have certain benefits for skin health.

[0035] Myocardial peptide powder is mainly extracted from the myocardial tissue of animals (such as pigs and cattle). Myocardial peptide powder can provide nutritional support for myocardial cells. It can promote the metabolism of myocardial cells and help cells absorb and utilize nutrients. In addition, when myocardial cells are damaged, myocardial peptide powder helps to start the repair mechanism of myocardial cells. It can stimulate the regeneration and repair of myocardial cells and promote the healing of damaged areas. This is achieved by regulating the signaling pathways within the cells and activating gene expression related to repair. Moreover, long-term use of myocardial peptide powder can enhance the contractility of the myocardium, allowing the heart to pump blood more efficiently. At the same time, it can also regulate the rhythm of the heart, help maintain a normal heart rate, and reduce the risk of arrhythmias.

[0036] In addition, cranberry powder is processed from cranberries, which are rich in various nutrients. These nutrients are retained after being made into powder, and mainly include antioxidant ingredients such as vitamin C, vitamin E, flavonoids, and anthocyanins. Among them, vitamin C is an important water-soluble vitamin that helps to enhance immunity and promote collagen synthesis; vitamin E is a fat-soluble vitamin that has antioxidant and cell membrane protection effects. Flavonoids and anthocyanins are powerful antioxidants in cranberry powder. Flavonoids can neutralize free radicals in the body and reduce the damage of free radicals to cells, thereby helping to prevent chronic diseases such as cardiovascular disease and cancer.

[0037] Anthocyanins give cranberries their unique red color. In addition to their antioxidant effects, they are also good for eye health, helping to improve vision and relieve eye fatigue. In addition, cranberry powder is rich in dietary fiber, which helps promote intestinal peristalsis, improve constipation, and increase satiety, which is beneficial for weight control. At the same time, it also contains some minerals that help maintain the normal function of the heart and muscles.

[0038] Therefore, the saffron drinking powder of the present invention includes the above-mentioned raw materials, which can make the raw materials have a mutually promoting effect, have the effect of "1+1>2", and has excellent free radical scavenging ability and protect myocardial function, thereby improving human cardiovascular and cerebrovascular problems, and can be used to prevent or treat cardiovascular diseases.

[0039] Among them, crocin and crocetin in saffron extract have antioxidant functions and can effectively remove free radicals in the body. Although myocardial peptide powder mainly acts on myocardial cells, the metabolic process in cells also produces free radicals, which may help maintain the antioxidant environment of cells to a certain extent, which is conducive to the effect of saffron extract. Cranberry powder is rich in antioxidants such as flavonoids and anthocyanins. These three ingredients work together to more comprehensively remove free radicals in the body, reduce the damage of free radicals to cells, and thus delay the aging process.

[0040] In addition, saffron extract has the function of regulating the nervous system and improving mood by regulating neurotransmitter levels. Myocardial peptide powder not only maintains normal heart function, but also indirectly has a positive effect on the nervous system, because the stability of heart function is important for the normal functioning of the nervous system. The nutrients in cranberry powder can provide nutritional support for the nervous system, and its antioxidant effect also helps protect nerve cells from oxidative damage.

[0041] In addition, cranberry powder is rich in nutrients such as vitamin C, vitamin E and dietary fiber, saffron extract also has its unique bioactive ingredients, and myocardial peptide powder can supplement protein-related nutrients (small molecule peptides) for the human body. These ingredients combined can provide the human body with a variety of nutrients and help enhance the body's immunity. Ingredients such as vitamin C can promote the function of immune system cells, and small molecule peptides can help the body repair and maintain normal cell metabolism, and jointly resist the invasion of pathogens.

[0042] Moreover, in a specific implementation, the weight portions of the raw materials of the saffron drinking powder are preferably: 25-30 parts of saffron extract, 20-30 parts of myocardial peptide powder, 0.1-5 parts of menthol, 0.1-5 parts of citric acid, 22-26 parts of erythritol, 10-15 parts of maltodextrin, and 10-15 parts of cranberry powder.

[0043] Here, the present invention is further described in conjunction with specific embodiments:

[0044] Example 1

[0045] A saffron direct drinking powder, wherein the weight portions of the raw materials are respectively:

[0046] 25.5 parts of saffron extract, 23 parts of myocardial peptide powder, 0.5 parts of menthol, 0.8 parts of citric acid, 25.2 parts of erythritol, 15 parts of maltodextrin, and 10 parts of cranberry powder.

[0047] The preparation method of the above-mentioned saffron direct drinking powder comprises:

[0048] S1. Weighing: weigh saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin, and cranberry powder according to weight;

[0049] S2. Granulation: The weighed raw materials are made into wet soft materials, and 95% ethanol is used as a binder for the wet granules to obtain uniform 50-mesh wet granules;

[0050] S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying, and controlling the moisture content to be no more than 5.0%, to obtain dried saffron direct drinking powder;

[0051] S4. Packaging: The dried saffron direct drinking powder is divided into small packages, and the composite film is first obtained, and the horizontal sealing temperature is adjusted to the preset temperature, and the inner packaging is carried out according to the filling amount of 1.2g per bag, and the weight difference is limited to ±7%. Then, based on the materials with qualified inner packaging and filling amount, the outer packaging is carried out according to the established process regulations, and the inspection is carried out according to the standards to ensure that the packaging is not damaged, without missing printing and other quality problems.

[0052] Example 2

[0053] A saffron direct drinking powder is composed of the following raw materials in parts by weight: 28 parts of saffron extract, 27 parts of myocardial peptide powder, 1 part of menthol, 1 part of citric acid, 23 parts of erythritol, 10 parts of maltodextrin and 10 parts of cranberry powder.

[0054] The preparation method of the saffron direct drinking powder of this embodiment is the same as that of Example 1.

[0055] Example 3

[0056] The invention discloses a saffron direct drinking powder, which is composed of the following raw materials in parts by weight: 30 parts of saffron extract, 25 parts of myocardial peptide powder, 1 part of menthol, 1 part of citric acid, 22 parts of erythritol, 10 parts of maltodextrin and 11 parts of cranberry powder.

[0057] The preparation method of the saffron direct drinking powder of this embodiment is the same as that of Example 1.

[0058] Example 4

[0059] The invention discloses a saffron direct drinking powder, which is composed of the following raw materials in parts by weight: 28 parts of saffron extract, 22 parts of myocardial peptide powder, 2 parts of menthol, 2 parts of citric acid, 22 parts of erythritol, 12 parts of maltodextrin and 12 parts of cranberry powder.

[0060] The preparation method of the saffron direct drinking powder of this embodiment is the same as that of Example 1.

[0061] Example 5

[0062] The invention discloses a saffron direct drinking powder, which is composed of the following raw materials in parts by weight: 25 parts of saffron extract, 22 parts of myocardial peptide powder, 2 parts of menthol, 2 parts of citric acid, 25 parts of erythritol, 12 parts of maltodextrin and 12 parts of cranberry powder.

[0063] The preparation method of the saffron direct drinking powder of this embodiment is the same as that of Example 1.

[0064] Example 6

[0065] A saffron direct drinking powder is composed of the following raw materials in parts by weight: 26 parts of saffron extract, 23 parts of myocardial peptide powder, 1 part of menthol, 1 part of citric acid, 24 parts of erythritol, 15 parts of maltodextrin and 10 parts of cranberry powder.

[0066] The preparation method of the saffron direct drinking powder of this embodiment is the same as that of Example 1.

[0067] Comparative Example 1

[0068] A direct drinking powder is composed of the following raw materials in parts by weight: 23 parts of myocardial peptide powder, 0.5 parts of menthol, 0.8 parts of citric acid, 25.2 parts of erythritol, 15 parts of maltodextrin, and 10 parts of cranberry powder.

[0069] The preparation method of the direct drinking powder comprises:

[0070] S1. Weighing: weigh myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin, and cranberry powder according to weight;

[0071] S2. Granulation: The weighed raw materials are made into wet soft materials, and 95% ethanol is used as a binder for the wet granules to obtain uniform 50-mesh wet granules;

[0072] S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying, and controlling the moisture content to be no more than 5.0%, to obtain dried saffron direct drinking powder;

[0073] S4, Packaging: The dried direct drinking powder is packaged into small packages, and the composite film is first obtained, and the horizontal sealing temperature is adjusted to the preset temperature, and the inner packaging is carried out according to the filling amount of 1.2g per bag, and the weight difference is limited to ±7%. Then, based on the materials with qualified inner packaging and filling amount, the outer packaging is carried out according to the established process regulations, and the inspection is carried out according to the standards to ensure that the packaging is not damaged, without missing printing and other quality problems.

[0074] Comparative Example 2

[0075] A direct drinking powder is composed of the following raw materials in parts by weight: 25.5 parts of saffron extract, 0.5 parts of menthol, 0.8 parts of citric acid, 25.2 parts of erythritol, 15 parts of maltodextrin, and 10 parts of cranberry powder.

[0076] The preparation method of the direct drinking powder of this embodiment is generally the same as that of Embodiment 1, except that the myocardial peptide powder is not weighed.

[0077] The preparation method of the direct drinking powder comprises:

[0078] S1. Weighing: weigh saffron extract, menthol, citric acid, erythritol, maltodextrin, and cranberry powder according to weight;

[0079] S2. Granulation: The weighed raw materials are made into wet soft materials, and 95% ethanol is used as a binder for the wet granules to obtain uniform 50-mesh wet granules;

[0080] S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying, and controlling the moisture content to be no more than 5.0%, to obtain dried saffron direct drinking powder;

[0081] S4, Packaging: The dried direct drinking powder is packaged into small packages, and the composite film is first obtained, and the horizontal sealing temperature is adjusted to the preset temperature, and the inner packaging is carried out according to the filling amount of 1.2g per bag, and the weight difference is limited to ±7%. Then, based on the materials with qualified inner packaging and filling amount, the outer packaging is carried out according to the established process regulations, and the inspection is carried out according to the standards to ensure that the packaging is not damaged, without missing printing and other quality problems.

[0082] Comparative Example 3

[0083] A direct drinking powder is composed of the following raw materials in parts by weight: 25.5 parts of saffron extract, 23 parts of myocardial peptide powder, 0.5 parts of menthol, 0.8 parts of citric acid, 25.2 parts of erythritol and 15 parts of maltodextrin.

[0084] The preparation method of the direct drinking powder comprises:

[0085] S1. Weighing: weigh saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, and maltodextrin according to weight;

[0086] S2. Granulation: The weighed raw materials are made into wet soft materials, and 95% ethanol is used as a binder for the wet granules to obtain uniform 50-mesh wet granules;

[0087] S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying, and controlling the moisture content to be no more than 5.0%, to obtain dried saffron direct drinking powder;

[0088] S4, Packaging: The dried direct drinking powder is packaged into small packages, and the composite film is first obtained, and the horizontal sealing temperature is adjusted to the preset temperature, and the inner packaging is carried out according to the filling amount of 1.2g per bag, and the weight difference is limited to ±7%. Then, based on the materials with qualified inner packaging and filling amount, the outer packaging is carried out according to the established process regulations, and the inspection is carried out according to the standards to ensure that the packaging is not damaged, without missing printing and other quality problems.

[0089] The following is an evaluation of the scavenging ability of the free radical DPPH by taking the preparation of saffron direct drinking powder in Example 1 as an example:

[0090] This example evaluates the ability of saffron direct drinking powder to protect cardiovascular system from free radical damage and anti-oxidation by comparing the ability of common antioxidants vitamin C (VC), vitamin E (VE) and saffron direct drinking powder to scavenge the free radical DPPH. At the same time, the salicylic acid method is also used to further determine their ability to scavenge hydroxyl free radicals, and comprehensively judge the antioxidant efficacy of each substance.

[0091] Using hydrogen peroxide (H2O2) and ferrous ions (Fe 2+ ) to produce hydroxyl radicals, the reaction equation is: H2O2+Fe 2+ → OH+H2O+Fe 3+ . Because in the pathological and physiological processes of redox reactions and inflammation in the body, there will also be similar free radical production mechanisms, and hydroxyl free radicals are highly active free radicals that can cause oxidative damage to cells and biological macromolecules, and are closely related to the occurrence and development of cardiovascular diseases.

[0092] Salicylic acid is then added to the above-mentioned system that produces hydroxyl radicals. Salicylic acid can capture hydroxyl radicals and react to produce colored substances, and the colored substances have a maximum absorption value at a specific wavelength (510nm). By using instruments such as a spectrophotometer to detect the absorbance value at this wavelength, the absorbance can be used to represent the relative content of hydroxyl radicals in the system. Because the absorbance is proportional to the concentration of the colored substance, and the amount of the colored substance produced is related to the amount of hydroxyl radicals, it can indirectly reflect the amount of hydroxyl radicals.

[0093] In addition, a specific clearance rate calculation formula was used to quantify the scavenging ability of each reagent (VC, VE, saffron drinking powder) on hydroxyl free radicals. The clearance rate calculation formula is: clearance rate = (A0-(A X -A X0 )) / A0×100%. A0 represents the absorbance of the blank control solution. The blank control solution is the absorbance measured when only hydroxyl radical generation and salicylic acid reaction are performed without adding the test solution (i.e. VC, VE, saffron direct drinking powder, etc.). It reflects the amount of hydroxyl radicals originally generated in the system.

[0094] A X It is the absorbance after adding the test solution, which reflects the absorbance corresponding to the remaining hydroxyl free radicals under the action of the reagent; A X0 It is the background absorption value of the test solution, that is, the possible absorbance of the test solution itself at the detection wavelength, which needs to be deducted to accurately reflect the effect of the reagent on hydroxyl free radicals.

[0095] from Figure 1 According to the results, vitamin C (VC) showed the strongest scavenging ability in terms of the ability to scavenge hydroxyl free radicals, indicating that it can efficiently capture and eliminate hydroxyl free radicals in this experimental system, thereby reducing the oxidative damage of free radicals to the system. Vitamin E (VE) has the worst scavenging ability. In comparison, the saffron direct drinking powder's ability to scavenge hydroxyl free radicals is comparable to VC, indicating that saffron direct drinking powder has a relatively outstanding performance in anti-oxidation and resistance to damage to cardiovascular and other tissues caused by hydroxyl free radicals, and has a strong potential to protect the cardiovascular system from free radical damage.

[0096] The following is an evaluation of the myocardial protection ability of saffron direct drinking powder:

[0097] Cell culture conditions: Rat embryonic cardiomyocyte cell line H9c2 was selected for the experiment, and DMEM high-glucose medium was used with 10% fetal bovine serum (FBS) added to provide the cells with sufficient nutrients and growth factors to enable them to grow well. The cells were incubated in an incubator at 37°C and 5% to maintain a suitable pH value of the culture medium, simulate the physiological environment in the body, and ensure that the cells were in an adherent growth state.

[0098] Cell inoculation: H9c2 cells in the logarithmic growth phase were selected and inoculated into a 96-well plate, and 150 μL of culture medium was added to each well. The reason for choosing cells in the logarithmic growth phase is that the cells have high viability, stable physiological characteristics and are in an active proliferation state at this time, which can give a more accurate and stable response to subsequent experimental treatments, and is more conducive to evaluating the myocardial protective effect of saffron extract.

[0099] Experimental group setting and treatment:

[0100] Normal group: The cells in this group were not disturbed by additional harmful factors during the entire experiment. They were not exposed to hydrogen peroxide (H2O2) that can cause cardiomyocyte damage, and no saffron extract was added. This was designed to reflect the state of cells under normal growth conditions and serve as a basic reference for subsequent comparisons.

[0101] Injury model (H2O2) group: Add H2O2 with a final concentration of 200μM to this group of cells for 1-3 hours (the specific duration is adjusted according to the number of cells inoculated). As a common oxidative stress inducer, it can induce intracellular redox imbalance and simulate the pathological conditions of oxidative damage to myocardial cells in vivo, thereby constructing a myocardial cell injury model and observing the changes in cell vitality under the injury state.

[0102] Dosing group (H2O2 + saffron direct drinking powder): There are a total of nine dosing groups corresponding to the above six embodiments and three comparative examples. Before adding H2O2, 50 μL of saffron extract control drug prepared in the corresponding embodiment was added to each well of each group of cells, and the cells were incubated in the incubator for 24 hours. The purpose is to allow the saffron extract to interact with the cells in advance and observe whether it can prevent or reduce the myocardial cell damage caused by the subsequent addition of H2O2, thereby evaluating the protective ability of saffron direct drinking powder on myocardial cells.

[0103] MTT addition and incubation: After the above different treatments are completed, add 5 mg / mL MTT solution to each well of the 96-well plate, and add 20 μL to each well. Then put the 96-well plate back into the incubator and continue incubating for 4 hours. During this period, the mitochondrial succinate dehydrogenase in the living cells will play a role. MTT can be reduced to water-insoluble Formazan crystals by the succinate dehydrogenase in the mitochondria of the living cells. The amount of Formazan crystals generated is positively correlated with the number and viability of living cells. The subsequent detection of the crystals can indirectly reflect the cell viability.

[0104] Dissolution and detection: After the incubation, discard the supernatant, add 150μL DMSO (dimethyl sulfoxide) to each well, shake for 15 minutes to fully dissolve the Formazan crystals in DMSO, and then use a microplate reader to measure the absorbance value (OD) at a wavelength of 570nm. The size of the OD value is directly related to the amount of Formazan crystals generated in the cells, which indirectly reflects the number of living cells and cell viability.

[0105] Data collection and processing: The average value of 3 parallel wells was taken for each detection point, and the experiment was repeated 3 times to reduce the experimental error and make the data more accurate and reliable. Finally, the cell survival rate was calculated according to the formula "cell survival rate % = (OD drug group-OD normal group) / (OD model group-OD normal group) × 100%". By comparing the cell survival rates of different groups, the protective effect of saffron extract on cardiomyocytes was intuitively evaluated through the following Table 1.

[0106] Table 1

[0107] Model Cell survival rate / % Normal group 90.13±3.86 <![CDATA[Model group (H2O2)]]> 46.57±5.92 <![CDATA[Administration Group 1 (H2O2 + Saffron Direct Drink Powder of Example 1)]]> 70.25±6.22 <![CDATA[Administration group 2 (H2O2 + saffron direct drinking powder of Example 2)]]> 70.56±4.68 <![CDATA[Administration group 3 (H2O2 + saffron direct drink powder of Example 3)]]> 69.78±3.21 <![CDATA[Administration group 4 (H2O2 + saffron direct-drinking powder of Example 4)]]> 69.86±5.42 <![CDATA[Administration group 5 (H2O2 + saffron direct drink powder of Example 5)]]> 70.32±3.48 <![CDATA[Administration group 6 (H2O2 + saffron direct drink powder of Example 6)]]> 69.38±5.24 <![CDATA[Administration group 7 (H2O2 + direct drinking powder of Comparative Example 1)]]> 51.25±6.1 <![CDATA[Administration group 8 (H2O2 + direct drinking powder of Comparative Example 2)]]> 61.65±3.55 <![CDATA[Administration group 9 (H2O2 + direct drinking powder of Comparative Example 3)]]> 68.48±1.23

[0108] From the cell survival rate data of each group presented in Table 1, it can be seen that:

[0109] The cell survival rate of the normal group was 90.13±3.86%, indicating that the cells maintained a high level of activity under normal culture conditions when not affected by damaging factors. This data can provide a benchmark reference for subsequent judgment of cell damage in other groups and the protective effect of drugs.

[0110] The cell survival rate of the injury model group (H2O2) was 46.57±5.92%: compared with the normal group, the cell survival rate of this group was significantly reduced, indicating that the addition of H2O2 did cause significant damage to the cardiomyocytes. A cardiomyocyte injury model was successfully constructed, simulating the decreased vitality of cardiomyocytes under pathological conditions such as oxidative stress.

[0111] The cell survival rate of the drug administration group was between 69% and 71%: compared with the injury model group, the cell survival rate of the drug administration group was improved. In particular, the cell survival rates of drug administration groups 1 to 6 including saffron extract were all above 69%, while the cell survival rate of drug administration group 7 without saffron extract was only about 51%, and the cell survival rate of drug administration group 8 without myocardial peptide powder was about 61%, and the cell survival rate of drug administration group 9 without cranberry powder was about 68%.

[0112] This clearly shows that saffron drinking powder containing saffron extract, myocardial peptide powder and cranberry powder can effectively reduce the damage caused by H2O2 to myocardial cells, play a protective role in myocardial cells, help maintain the vitality of myocardial cells when they are damaged by oxidative stress, and improve their survival rate.

[0113] In summary, through this MTT experiment based on H9c2 cells and the corresponding data analysis, it is fully proved that the saffron direct drinking powder of this example has a protective effect and can be used to prevent or treat cardiovascular diseases.

[0114] The above-mentioned embodiments only express the preferred implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for a person skilled in the art, several modifications, improvements and substitutions can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the attached claims.

Claims

1. A saffron direct drinking powder, characterized in that: The raw materials of the saffron direct drinking powder include saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin and cranberry powder.

2. The saffron direct drinking powder according to claim 1, characterized in that The weight parts of each raw material are respectively: 25-30 parts of saffron extract, 20-30 parts of myocardial peptide powder, 0.1-5 parts of menthol, 0.1-5 parts of citric acid, 22-26 parts of erythritol, 10-15 parts of maltodextrin, and 10-15 parts of cranberry powder.

3. A method for preparing the saffron drinking powder according to claim 1 or 2, characterized in that: The following steps are involved: S1. Weighing: weigh saffron extract, myocardial peptide powder, menthol, citric acid, erythritol, maltodextrin, and cranberry powder according to weight; S2, granulation: the weighed raw materials are made into wet soft materials to obtain uniform wet granules; S3, granulation: placing the wet granules obtained by granulation into a hot air circulation oven for drying to obtain dried saffron direct drinking powder; S4. Packaging: Divide the dried saffron drinking powder into small packages.

4. The method for preparing saffron direct drinking powder according to claim 3, characterized in that: In step S2, the binder of the wet granules is 95% ethanol.

5. The method for preparing saffron direct drinking powder according to claim 3, characterized in that: In step S2, the particle size of the wet granules is 40-60 mesh.

6. The method for preparing saffron direct drinking powder according to claim 3, characterized in that: In step S3, the water content of the dried saffron drinking powder is no more than 5.0%.

7. The method for preparing saffron direct drinking powder according to claim 3, characterized in that: Step S4 includes: S41, inner packaging: receive the composite film, adjust the transverse sealing temperature to the preset temperature, and perform inner packaging according to the preset weight of each bag; S42. Outer packaging: Carry out outer packaging in accordance with process regulations and inspect according to standards.

8. The method for preparing saffron direct drinking powder according to claim 7, characterized in that: In step S41, the preset weight is 1.2 g / bag, and the weight difference limit requirement is ±7%.

9. The use of saffron direct drinking powder according to claim 1 or 2, characterized in that: The saffron direct drinking powder is used for preventing or treating cardiovascular diseases.

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