Countercurrent electrophoresis separation device

Abstract

The embodiment of the invention provides a countercurrent electrophoretic separation device. The countercurrent electrophoretic separation device comprises a power supply assembly, a separation chamber and an electrode chamber, the separation chamber is provided with a feeding port, a discharging port and a separation channel, and the feeding port and the discharging port are located in the two opposite ends of the separation channel correspondingly and communicate with the separation channel. The electrode chambers comprise the first electrode chamber and the second electrode chamber, the first electrode chamber is located at the end, close to the feeding port, of the separation channel to communicate with the separation channel, the second electrode chamber is located at the end, close to the discharging port, of the separation channel to communicate with the separation channel, and the first electrode chamber is electrically connected with the negative electrode of the power source assembly; the second electrode chamber is electrically connected with the anode of the power supply assembly. The countercurrent electrophoresis separation device disclosed by the embodiment of the invention has a relatively good separation effect.

Description

Technical Field

[0001] The present application relates to the technical field of isotope separation, and in particular to a countercurrent electrophoresis separation device. Background Art

[0002] 90 Strontium is a radioactive isotope with a half-life of 28 years. 90 Yttrium, strontium and yttrium are very easy to balance. 90 Yttrium is a β-decay element with a half-life of 64.0 h and a maximum β-ray energy of 2.28 MeV. Its decay products are stable elements. 90 These properties make Zr. 90 Yttrium has broad application prospects in nuclear medicine.

[0003] at present 90 Yttrium is mainly produced through 90 strontium / 90 Yttrium generator acquisition, however, in the related art 90 Strontium and 90 The separation effect of yttrium is poor. Summary of the invention

[0004] In view of this, the main purpose of the embodiments of the present application is to provide a countercurrent electrophoresis separation device with better separation effect.

[0005] To achieve the above purpose, the technical solution of the embodiment of the present application is implemented as follows:

[0006] The present application provides a countercurrent electrophoresis separation device, comprising:

[0007] Power supply components;

[0008] A separation chamber, wherein the separation chamber has a feed inlet, a discharge port and a separation channel, wherein the feed inlet and the discharge port are respectively located at opposite ends of the separation channel and are both communicated with the separation channel;

[0009] The electrode chamber includes a first electrode chamber and a second electrode chamber, the first electrode chamber is located at one end of the separation channel close to the feed port to communicate with the separation channel, the second electrode chamber is located at one end of the separation channel close to the discharge port to communicate with the separation channel, the first electrode chamber is electrically connected to the negative electrode of the power supply component, and the second electrode chamber is electrically connected to the positive electrode of the power supply component.

[0010] In one embodiment, the countercurrent electrophoresis separation device includes a plurality of cation exchange membranes, and the cation exchange membranes are respectively disposed at the connection point between the first electrode chamber and the separation chamber, and at the connection point between the second electrode chamber and the separation chamber.

[0011] In one embodiment, the separation channel has a separation zone and a buffer zone, the buffer zone is arranged on a side of the separation zone close to the first electrode chamber and is connected to the first electrode chamber, one end of the separation zone is connected to the second electrode chamber, and the other end of the separation zone is connected to the buffer zone.

[0012] In one embodiment, the cross-sectional area of ​​the buffer zone is larger than the cross-sectional area of ​​the separation zone.

[0013] In one embodiment, the electrode chamber is filled with an electrode solution containing a pH adjuster.

[0014] In one embodiment, the electrode solution is a first phosphoric acid solution;

[0015] The pH of the first phosphoric acid solution is 3; and / or,

[0016] The concentration of the first phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L.

[0017] In one embodiment, the countercurrent electrophoresis separation device is used to separate a sample solution, the separation channel is filled with the sample solution and a buffer solution, and the buffer solution is used to drive the target ions in the sample solution to be discharged from the discharge port.

[0018] In one embodiment, the buffer solution is a second phosphoric acid solution having a pH adjuster and / or a complexing agent.

[0019] In one embodiment, the pH of the second phosphoric acid solution is 3; and / or,

[0020] The concentration of the second phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L.

[0021] In one embodiment, the output voltage of the power supply assembly is greater than or equal to 200V and less than or equal to 20000V; and / or,

[0022] The separation chamber is made of one of organic glass, oxide ceramics and boron nitride materials; and / or,

[0023] The countercurrent electrophoresis separation device comprises a first electrode and a second electrode, the first electrode is electrically connected to the first electrode chamber and the negative electrode of the power supply assembly respectively, the second electrode is electrically connected to the second electrode chamber and the positive electrode of the power supply assembly respectively, at least one of the first electrode and the second electrode is one of a platinum sheet electrode, a platinum wire electrode and a graphite rod electrode; and / or,

[0024] The countercurrent electrophoresis separation device is used to separate a sample solution, wherein the sample solution contains 90 Yttrium and 90 Solutions of strontium and 99 Technetium and 99 One of the solutions of molybdenum.

[0025] The embodiment of the present application provides a countercurrent electrophoresis separation device, which includes a power supply assembly, a separation chamber and an electrode chamber. The separation chamber has a feed inlet, a discharge port and a separation channel, and the feed inlet and the discharge port are respectively located at opposite ends of the separation channel, and are both connected to the separation channel. The electrode chamber includes a first electrode chamber and a second electrode chamber, the first electrode chamber is located at one end of the separation channel close to the feed inlet to communicate with the separation channel, the second electrode chamber is located at one end of the separation channel close to the discharge port to communicate with the separation channel, the first electrode chamber is electrically connected to the negative electrode of the power supply assembly, and the second electrode chamber is electrically connected to the positive electrode of the power supply assembly. By setting the direction of the electric field in the opposite direction to the flow direction of the solution, the charged particles with higher electrical mobility are retained in the separation channel by the electric field, and the charged particles with lower electrical mobility will flow out of the discharge port with the solution, thereby achieving the separation of the two charged particles, which has a better separation effect, while also reducing the generation of radioactive waste, shortening the overall separation process, and improving the efficiency of the separation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic structural diagram of a countercurrent electrophoresis separation device according to an embodiment of the present application.

[0027] Description of Reference Numerals

[0028] 10. Separation chamber; 10a. Feed inlet; 10b. Feed outlet; 10c. Separation zone; 10d. Buffer zone; 20. Electrode chamber; 20a. Liquid inlet; 20b. Liquid outlet; 21. First electrode chamber; 22. Second electrode chamber; 30. Cation exchange membrane; 40. First electrode; 50. Second electrode. DETAILED DESCRIPTION

[0029] Related technologies include a 90 strontium / 90 Yttrium generator. The generator includes a first chromatographic column, a liquid storage tank, and a second chromatographic column. Polyantimony acid with a mesh size of 60 to 120 is selected as the separation material in the chromatographic column. First, a strontium nitrate solution is loaded into the first chromatographic column; then, hydrochloric acid is used as an eluent and an eluent to elute and elute the first chromatographic column; then, the primary eluent is mixed with a reverse carrier solution and passed through a non-loaded strontium nitrate column. 90 A second chromatographic column of strontium solution is prepared to obtain 90 Yttrium nuclide. After one separation 90 strontium / 90The yttrium activity ratio is about 10 -4 , after secondary separation 90 strontium / 90 Yttrium activity ratio reaches 10 -7 Although the product can meet medical standards, the operation process is complicated and produces a large amount of radioactive waste that is difficult to handle. In addition, the service life and quality of the chromatographic column vary and cannot be guaranteed. 90 strontium / 90 Stability of the yttrium generator.

[0030] Among related technologies, capillary electrophoresis has developed rapidly in the field of charged ion separation research, confirming the basic theory that different charged ions have different electrical mobilities. Researchers have obtained a lot of ion mobility data through capillary electrophoresis. For example, the electrical mobility of strontium ions is 68×10 -5 cm 2 / V·s, the electrical mobility of yttrium ions is 37×10 -5 cm 2 / V·s. However, capillary electrophoresis technology is still limited to detection and separation, and cannot obtain quantitative high-purity ion products.

[0031] The related art provides a countercurrent electrophoresis experimental scheme for separating rubidium ions from cesium ions, expanding the thickness of the electrolytic cell cavity to the millimeter level and the capacity of the electrolytic cell to the milliliter level. The main working principle of countercurrent electrophoresis is to apply a fluid flow rate in the opposite direction while applying a voltage. When the fluid flow rate is set between the electrophoretic rates of the components to be separated, particles with higher electrophoretic mobility are retained by the electric field, while compounds with lower electrophoretic rates will flow through the channel with the liquid flow. By properly adjusting the current / flow rate ratio, the molecular factor of rubidium ions and cesium ions can be stabilized at 2×10 -3 The separation chamber of the device is designed as an open system, which allows any bubbles that may form in the electrolyte layer to escape easily from this open system. However, it cannot ensure a constant liquid level, is prone to overflow, and is difficult to ensure a uniform flow profile of the separated substances in the flow direction, that is, a constant ion concentration distribution perpendicular to the flow direction, and the separation coefficient still needs to be improved.

[0032] Furthermore, there is currently no application of countercurrent electrophoresis technology to 90 strontium / 90 Studies on the separation of yttrium.

[0033] It should be noted that 90 Strontium has a bone-seeking property, which means it tends to accumulate in bones. Its maximum permissible carry-over amount (MPBB) is 74 kBq (2 lCi). Assuming a patient can receive a maximum of 37 GBq in their lifetime, 90 Yttrium, then 37GBq 90 Yttrium 90Strontium should not exceed 74 kBq. 90 Yttrium needs to be of very high radionuclide purity (>99.998%). According to the USP, 90 Yttrium radiopharmaceuticals, 90 strontium / 90 The yttrium activity ratio should not exceed 1×10 -6 .

[0034] An embodiment of the present application provides a countercurrent electrophoresis separation device, see Figure 1 The countercurrent electrophoresis separation device includes a power supply component, a separation chamber 10 and an electrode chamber 20.

[0035] The separation chamber 10 has a feed port 10a, a discharge port 10b and a separation channel. The feed port 10a and the discharge port 10b are respectively located at two opposite ends of the separation channel and are both communicated with the separation channel.

[0036] The electrode chamber 20 includes a first electrode chamber 21 and a second electrode chamber 22. The first electrode chamber 21 is located at one end of the separation channel close to the feed port 10a to communicate with the separation channel. The second electrode chamber 22 is located at one end of the separation channel close to the discharge port 10b to communicate with the separation channel. The first electrode chamber 21 is electrically connected to the negative electrode of the power supply component, and the second electrode chamber 22 is electrically connected to the positive electrode of the power supply component.

[0037] Specifically, the countercurrent electrophoresis separation device refers to a device that uses the principle of electrophoresis to separate substances.

[0038] There is no limit to the types of substances that can be separated by the countercurrent electrophoresis separation device.

[0039] For example, the countercurrent electrophoresis separation device is used to separate the sample solution. The separation channel is filled with the sample solution and the buffer solution. The buffer solution is used to drive the target ions in the sample solution to be discharged from the discharge port 10b.

[0040] The power supply assembly refers to a component that connects the first electrode chamber 21 and the second electrode chamber 22 to form an electric field. According to the different charged particles, the magnitude of the output voltage of the power supply assembly is adjusted to control the electric field strength, thereby affecting the electrophoresis rate of different charged particles in the separation channel, so as to achieve effective separation of different charged particles.

[0041] The type of charged particles is not limited.

[0042] For example, a charged particle is 90 Yttrium and 90 Strontium, countercurrent electrophoresis separation device is used to separate the sample solution, the sample solution contains 90 Yttrium and 90 Strontium solution.

[0043] For example, a charged particle is 99 molybdenum / 99 Technetium, countercurrent electrophoresis separation device is used to separate the sample solution, the sample solution contains 99 molybdenum / 99 Technetium solution.

[0044] For ease of description, this application uses a countercurrent electrophoresis separation device to separate 90 Yttrium and 90 The following description will be made taking strontium as an example.

[0045] The voltage output by the power supply component can be set according to actual conditions.

[0046] Exemplarily, the output voltage of the power supply component is greater than or equal to 200V and less than or equal to 20000V. For example, the output voltage of the power supply component is 200V, 1000V, 2000V, 5000V, 10000V, 15000V or 20000V. Maintaining the output voltage of the power supply component within the above range can make 90 Yttrium and 90 Strontium separation is better.

[0047] The separation chamber 10 refers to the sample solution 90 Yttrium and 90 The site for strontium separation.

[0048] The feed port 10 a refers to an inlet through which the sample solution enters the separation chamber 10 .

[0049] The separation channel is used to separate the sample solution. 90 Yttrium and 90 Strontium channel.

[0050] The outlet 10b refers to the outlet of the sample solution containing 90 Outlet for yttrium solution.

[0051] The sample solution enters the separation chamber 10 from the feed port 10a, and a solution flow path from the feed port 10a to the discharge port 10b is formed in the separation channel. The power supply component is electrically connected to the first electrode chamber 21 and the second electrode chamber 22 to form an electric field. 90 Yttrium and 90 Under the action of the electric field, strontium moves in the separation channel in the opposite direction to the solution flow. 90 Yttrium and 90 Strontium has different electrical mobilities, with the lower electrical mobility 90 Yttrium flows out from the outlet 10b along with the sample solution, and the 90 Strontium is retained in the separation channel, thereby achieving 90 Yttrium and 90Separation of Strontium.

[0052] The manner in which the first electrode chamber 21 and the second electrode chamber 22 are electrically connected to the power supply assembly is not limited.

[0053] Exemplarily, the countercurrent electrophoresis separation device includes a first electrode 40 and a second electrode 50, the first electrode 40 is electrically connected to the first electrode chamber 21 and the negative electrode of the power supply assembly, respectively, the second electrode 50 is electrically connected to the second electrode chamber 22 and the positive electrode of the power supply assembly, and at least one of the first electrode 40 and the second electrode 50 is one of a platinum sheet electrode, a platinum wire electrode, and a graphite rod electrode. Thus, a stable electric field can be effectively established between the first electrode chamber 21 and the second electrode chamber 22, so that 90 Yttrium and 90 The stable migration of strontium in the separation channel improves 90 Yttrium and 90 Separation effect of strontium.

[0054] Specifically, the first electrode 40 may be one of a platinum sheet electrode, a platinum wire electrode and a graphite rod electrode.

[0055] The second electrode 50 may be one of a platinum sheet electrode, a platinum wire electrode and a graphite rod electrode.

[0056] The material type of the separation chamber 10 is not limited.

[0057] For example, the separation chamber 10 is made of one of organic glass, oxide ceramics and boron nitride materials, thereby preventing the separation chamber 10 material from chemically reacting with the solution and affecting the migration environment and separation effect of the ions.

[0058] The connection method between the separation chamber 10 and the first electrode chamber 21 and the second electrode chamber 22 is not limited.

[0059] For example, the separation chamber 10 is connected to the first electrode chamber 21 and the second electrode chamber 22 respectively by means of grooves, and the connection between the separation chamber 10 and the first electrode chamber 21 and the second electrode chamber 22 is fixed by bolts.

[0060] The countercurrent electrophoresis separation device of the embodiment of the present application includes a power supply assembly, a separation chamber 10 and an electrode chamber 20. The separation chamber 10 has a feed port 10a, a discharge port 10b and a separation channel, and the feed port 10a and the discharge port 10b are respectively located at opposite ends of the separation channel, and are both connected to the separation channel. The electrode chamber 20 includes a first electrode chamber 21 and a second electrode chamber 22, the first electrode chamber 21 is located at one end of the separation channel close to the feed port 10a to communicate with the separation channel, the second electrode chamber 22 is located at one end of the separation channel close to the discharge port 10b to communicate with the separation channel, the first electrode chamber 21 is electrically connected to the negative electrode of the power supply assembly, and the second electrode chamber 22 is electrically connected to the positive electrode of the power supply assembly. By setting the direction of the electric field in the opposite direction to the flow direction of the solution, the charged particles with higher electrical mobility are retained in the separation channel by the electric field, while the charged particles with lower electrical mobility will flow out from the discharge port 10b with the solution. In this way, the separation of the two charged particles can be achieved, which has a better separation effect, while also reducing the generation of radioactive waste, shortening the overall separation process, and improving the separation process efficiency.

[0061] In one embodiment, please refer to Figure 1 The countercurrent electrophoresis separation device includes a plurality of cation exchange membranes 30, and the cation exchange membranes 30 are respectively arranged at the connection between the first electrode chamber 21 and the separation chamber 10, and at the connection between the second electrode chamber 22 and the separation chamber 10. Thus, the bubbles in the first electrode chamber 21 and the second electrode chamber 22 can be blocked by the cation exchange membranes 30, and the peristalsis of the fluid in the separation chamber 10 is made more stable.

[0062] In one embodiment, please refer to Figure 1 , the separation channel has a separation zone 10c and a buffer zone 10d. The buffer zone 10d is arranged on the side of the separation zone 10c close to the first electrode chamber 21 and is connected to the first electrode chamber 21. One end of the separation zone 10c is connected to the second electrode chamber 22, and the other end of the separation zone 10c is connected to the buffer zone 10d. Therefore, by setting the buffer zone 10d, on the one hand, the process of the sample solution entering the separation chamber 10 from the discharge port 10b can be made smoother, turbulence can be avoided, and the instability of ion migration caused by sudden impact of the solution can be reduced, thereby improving the stability of the entire separation process, which is conducive to obtaining a stable separation effect and a high-purity separation product. On the other hand, the buffer zone 10d is for the sample solution enriched in the first electrode chamber 21. 90 The strontium solution provides storage space to avoid 90 Too much strontium can cause 90 Yttrium and 90 The separation effect of strontium is poor.

[0063] Specifically, the separation zone 10c refers to the 90 Yttrium and 90 The site for strontium separation.

[0064] In sample solution 90 Yttrium and 90 Strontium is separated in the separation zone 10c. Under the action of the electric field, 90 Yttrium flows to one side of the second ionization chamber and flows out from the outlet 10b along with the sample solution. 90 Strontium flows toward the side close to the first ionization chamber and is enriched in the buffer zone 10d.

[0065] The buffer zone 10d is used to store the enriched liquid on the side of the separation chamber 10 close to the first electrode chamber 21. 90 Strontium solution.

[0066] The structural type of the separation zone 10c is not limited.

[0067] For example, the separation zone 10c is cylindrical, and the radial length of its inner diameter is greater than or equal to 1 mm and less than or equal to 5 mm. For example, the radial length of the separation zone 10c is 1 mm, 3 mm or 5 mm, thereby being able to better balance the flow rate of the solution and the diffusion of ions.

[0068] In a specific embodiment, along the flow direction of the sample solution, the length of the separation zone 10c is greater than or equal to 240 mm and less than or equal to 300 mm. For example, the length of the separation zone 10c is 240 mm, 270 mm or 300 mm. Maintaining the length of the separation zone 10c within the above range can ensure that the sample solution 90 Yttrium and 90 Strontium is fully separated, thereby improving 90 Yttrium and 90 Separation effect of strontium.

[0069] The structure type of the buffer zone 10d is not limited.

[0070] For example, the buffer zone 10d is cylindrical and extends vertically. The radial length of the buffer zone 10d is greater than or equal to 25 mm and less than or equal to 35 mm. For example, the radial length of the buffer zone 10d is 25 mm, 30 mm or 35 mm.

[0071] For another example, the buffer zone 10d is arranged in a horizontal manner like a volumetric bottle, and the buffer zone 10d extends in the horizontal direction.

[0072] For another example, the buffer zone 10d extends laterally, and its cross-sectional shape is an ellipse elongated at both ends, that is, the major axis of the ellipse extends vertically, and an opening is formed at the lower end of the buffer zone 10d. The buffer zone 10d adopts the above structural shape without a cavity.

[0073] In a specific embodiment, the volume of the buffer zone 10d is greater than or equal to 3 ml and less than or equal to 7 ml. For example, the volume of the buffer zone 10d is 3 ml, 5 ml or 7 ml. 90 Strontium solutions have better storage effects.

[0074] In one embodiment, please refer to Figure 1 The cross-sectional area of ​​the buffer zone 10d is larger than the cross-sectional area of ​​the separation zone 10c. 90 Strontium enters the separation zone 10c again, thereby affecting the 90 Yttrium and 90 Separation effect of strontium.

[0075] 5mL 90 The injection time of the strontium solution is estimated by the volume of the buffer 10d and the flow rate of the injection pump fluid. For example:

[0076] Unit: m 3 / s;

[0077] The unit is s.

[0078] In one embodiment, the electrode chamber 20 is filled with an electrode liquid having a pH adjuster, so that the electrode liquid in the electrode chamber 20 can maintain a consistent pH value with the solution in the separation chamber 10, and the species of the electrode liquid in the electrode chamber 20 and the solution in the separation chamber 10 can also be consistent, thereby ensuring conductivity.

[0079] Specifically, the type of pH adjuster is not limited as long as it can adjust the pH of the electrode solution.

[0080] For example, the pH adjuster may be phosphoric acid-phosphate at a concentration of 0.01 to 0.08 mol / L, acetic acid-acetate at a concentration of 0.01 to 0.08 mol / L, or oxalic acid-oxalate at a concentration of 0.01 to 0.08 mol / L.

[0081] For another example, the pH adjuster is phosphoric acid-phosphate with a concentration of 0.05 mol / L, and the electrode liquid is the first phosphoric acid solution.

[0082] In one embodiment, the pH of the first phosphoric acid solution is 3. Thus, the electrode solution in the electrode chamber 20 can maintain a consistent pH value with the solution in the separation chamber 10, and the species of the electrode solution in the electrode chamber 20 and the solution in the separation chamber 10 can also be consistent, thereby ensuring conductivity.

[0083] In one embodiment, the concentration of the first phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L. For example, the concentration of the first phosphoric acid solution is 0.01 mol / L, 0.03 mol / L, 0.05 mol / L or 0.08 mol / L. This helps maintain the conductivity of the solution, reduces the resistance during ion migration, makes the charge transfer on the electrode surface more efficient, and thus improves the rate and efficiency of the electrode reaction.

[0084] In one embodiment, the buffer solution is a second phosphoric acid solution having a pH regulator and / or a complexing agent. Thus, by adding a pH regulator to the second phosphoric acid solution, the stability of the pH can be ensured, and the species forms of the strontium ions and yttrium ions in the solution can be maintained. By adding a complexing agent to the second phosphoric acid solution, the complexing agent can complex with the metal ions, change the electrical properties of the ion groups, and achieve a better separation effect.

[0085] Specifically, the second phosphoric acid solution may contain only a pH adjuster, only a complexing agent, or both a pH adjuster and a complexing agent.

[0086] It should be noted that the specific type of the complexing agent can be set according to actual conditions.

[0087] For example, the complexing agent is at least one of ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, ethylenediaminetetramethylenephosphonic acid, citric acid and α-hydroxyisobutyric acid. In other words, the complexing agent can be one of the above substances or a combination of the above substances.

[0088] In a specific embodiment, the buffer solution may be a phosphate buffer solution with a pH of 3, 0.016 mol / L ethylenediaminetetraacetic acid (EDTA) and a concentration of 0.03 mol / L.

[0089] In one embodiment, the pH of the second phosphoric acid solution is 3. Thus, the species forms of strontium ions and yttrium ions in the solution can be better maintained.

[0090] In one embodiment, the concentration of the second phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L. For example, the concentration of the second phosphoric acid solution is 0.01 mol / L, 0.03 mol / L, 0.05 mol / L or 0.08 mol / L. This helps maintain the conductivity of the solution, reduces the resistance during ion migration, makes the charge transfer on the electrode surface more efficient, and thus improves the rate and efficiency of the electrode reaction.

[0091] In one embodiment, the concentration of the complexing agent in the buffer solution is greater than or equal to 0.001 mol / L and less than or equal to 0.05 mol / L, such as 0.001 mol / L, 0.01 mol / L or 0.05 mol / L. Thus, the separation effect can be better.

[0092] In a specific embodiment, the buffer solution is a 0.03 mol / L phosphate buffer solution with a pH of 3 and containing 0.016 mol / L EDTA, which can further improve the separation effect.

[0093] In one embodiment, the countercurrent electrophoresis separation device further includes a plurality of storage tanks and a sampling pump, each storage tank respectively stores an electrode solution (pH=3, a phosphate buffer solution with a concentration of 0.05 mol / L), a separation chamber 10 buffer solution (pH=3, a phosphate buffer solution with a concentration of 0.03 mol / L having 0.08 mol / L ethylenediaminetetraacetic acid (EDTA)) and a 0.016 mol / L 90 The feed port 10a of the separation chamber 10 is connected to the strontium solution. 90 Yttrium 90 The strontium solution storage tank is connected and powered by a sample pump. 90 Yttrium 90 The strontium solution is transported from the feed port 10a to the separation chamber 10, and the sample solution is separated by the countercurrent electrophoresis separation device. 90 Yttrium 90 The separation factor of strontium is 1×10 -7 .

[0094] The fluid flow rate of the injection pump can be set according to actual conditions.

[0095] For example, the fluid flow rate of the injection pump is greater than or equal to 0.00003 m / s and less than or equal to 0.003 m / s. For example, 0.00003 m / s, 0.00006 m / s, 0.0001 m / s, 0.001 m / s, 0.002 m / s or 0.003 m / s. By controlling the fluid flow rate of the injection pump to the above values, the first ion and the second ion can be better separated.

[0096] There may also be a corresponding relationship between the fluid flow rate of the injection pump and the voltage of the power supply assembly.

[0097] For example, the relationship between the fluid flow rate of the injection pump and the voltage of the power supply component satisfies the following formula, as follows:

[0098]

[0099] in, vis the fluid flow rate of the injection pump, in m / s; U is the voltage of the power supply component; L is the length of the separation channel, in cm. Thus, the first ion and the second ion can be separated well.

[0100] In one embodiment, the countercurrent electrophoresis separation device includes a discharge pipeline, a control valve and a collection system. The discharge port 10b is connected to the collection system through the discharge pipeline. The collection system is used to collect 90 Yttrium 90 For the solution after strontium separation, a control valve is arranged on the discharge pipeline to detect the concentration of the solution flowing out of the discharge pipeline and control the on-off of the discharge pipeline.

[0101] In one embodiment, the electrode chamber 20 has a liquid inlet 20a and a liquid outlet 20b, and the liquid outlet 20b is arranged above the liquid inlet 20a along the height direction of the electrode chamber 20, and the electrode liquid enters the electrode chamber 20 from the liquid inlet 20a. Thus, the substance of the electrode liquid in the electrode chamber 20 can be made uniform, so that the conductivity is stable.

[0102] In the description of the present application, the description with reference to the terms "in one embodiment", "in some embodiments", "in a specific embodiment", or "exemplary" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiments of the present application. In the present application, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art may combine different embodiments or examples described in the present application and the features of different embodiments or examples without contradiction.

[0103] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application are included in the protection scope of the present application.

Claims

1. A countercurrent electrophoresis separation device, characterized in that: include: Power supply components; A separation chamber, wherein the separation chamber has a feed inlet, a discharge port and a separation channel, wherein the feed inlet and the discharge port are respectively located at opposite ends of the separation channel and are both communicated with the separation channel; The electrode chamber includes a first electrode chamber and a second electrode chamber, the first electrode chamber is located at one end of the separation channel close to the feed port to communicate with the separation channel, the second electrode chamber is located at one end of the separation channel close to the discharge port to communicate with the separation channel, the first electrode chamber is electrically connected to the negative electrode of the power supply component, and the second electrode chamber is electrically connected to the positive electrode of the power supply component.

2. The countercurrent electrophoresis separation device according to claim 1, characterized in that: The countercurrent electrophoresis separation device comprises a plurality of cation exchange membranes, and the cation exchange membranes are respectively arranged at the connection point between the first electrode chamber and the separation chamber, and at the connection point between the second electrode chamber and the separation chamber.

3. The countercurrent electrophoresis separation device according to claim 1, characterized in that: The separation channel has a separation zone and a buffer zone. The buffer zone is arranged on a side of the separation zone close to the first electrode chamber and is connected to the first electrode chamber. One end of the separation zone is connected to the second electrode chamber, and the other end of the separation zone is connected to the buffer zone.

4. The countercurrent electrophoresis separation device according to claim 3, characterized in that: The cross-sectional area of ​​the buffer zone is greater than the cross-sectional area of ​​the separation zone.

5. The countercurrent electrophoresis separation device according to any one of claims 1 to 4, characterized in that: The electrode chamber is filled with an electrode solution containing a pH adjuster.

6. The countercurrent electrophoresis separation device according to claim 5, characterized in that: The electrode liquid is a first phosphoric acid solution; The pH of the first phosphoric acid solution is 3; and / or, The concentration of the first phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L.

7. The countercurrent electrophoresis separation device according to any one of claims 1 to 4, characterized in that: The countercurrent electrophoresis separation device is used to separate a sample solution. The separation channel is filled with the sample solution and a buffer solution. The buffer solution is used to drive the target ions in the sample solution to be discharged from the discharge port.

8. The countercurrent electrophoresis separation device according to claim 7, characterized in that: The buffer solution is a second phosphoric acid solution having a pH adjuster and / or a complexing agent.

9. The countercurrent electrophoresis separation device according to claim 8, characterized in that: The pH of the second phosphoric acid solution is 3; and / or, The concentration of the second phosphoric acid solution is greater than or equal to 0.01 mol / L and less than or equal to 0.08 mol / L.

10. The countercurrent electrophoresis separation device according to any one of claims 1 to 4, characterized in that: The output voltage of the power supply assembly is greater than or equal to 200V and less than or equal to 20000V; and / or, The separation chamber is made of one of organic glass, oxide ceramics and boron nitride materials; and / or, The countercurrent electrophoresis separation device comprises a first electrode and a second electrode, the first electrode is electrically connected to the first electrode chamber and the negative electrode of the power supply assembly respectively, the second electrode is electrically connected to the second electrode chamber and the positive electrode of the power supply assembly respectively, at least one of the first electrode and the second electrode is one of a platinum sheet electrode, a platinum wire electrode and a graphite rod electrode; and / or, The countercurrent electrophoresis separation device is used to separate a sample solution, wherein the sample solution contains 90 Yttrium and 90 Solutions of strontium and containing 99 Technetium and 99 One of the solutions of molybdenum.

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