Microbial fertilizer as well as preparation method and application thereof

Abstract

The invention discloses a microbial fertilizer as well as a preparation method and application thereof. The microbial fertilizer comprises the following components: a precipitate of streptomyces lavendulae Hainan variant fermentation liquor, an inclusion agent, an adsorbent, a filler matrix, a carbon source, a nitrogen source and inorganic salt. The microbial fertilizer provided by the invention has good storage stability and an effect of promoting plant growth.

Description

Technical Field

[0001] The invention belongs to the field of microbial fertilizers, and in particular relates to a microbial fertilizer and a preparation method and application thereof. Background Art

[0002] Zhongshengmycin is a new type of agricultural antibiotic produced by the Hainan variant of Streptomyces lilacinus, which has high activity against bacterial diseases and some fungal diseases of crops. For a long time, after the fermentation liquid in the production process of Zhongshengmycin is extracted, the remaining fermentation liquid residue is still rich in a large number of live bacteria of the Hainan variant of Streptomyces lilacinus, as well as crude protein, polysaccharides, monosaccharides and amino acids and other biologically active substances necessary for plant growth. If all of them are treated as waste, it will consume a lot of manpower and material resources, be unfavorable to the reduction of consumption and emission in the production process, be unfavorable to environmental friendliness and compatibility, and be unfavorable to sustainable development.

[0003] Therefore, there is an urgent need for an energy-saving, environmentally friendly microbial fertilizer and a preparation method thereof in the art. Summary of the invention

[0004] In order to overcome the technical defects of the existing lilac gray Streptomyces Hainan variant biological fermentation production of secondary metabolites of mycotoxin biological pesticides that are not conducive to reducing consumption and emissions and are not conducive to environmental friendliness and compatibility, the present invention provides a microbial fertilizer and its preparation method and application. The microbial fertilizer can achieve recycling and clean production in the biological pesticide production process of mycotoxin in the secondary metabolites of lilac gray Streptomyces Hainan variant biological fermentation; at the same time, expand the market share of mycotoxin in the biological pesticide of the lilac gray Streptomyces Hainan variant series of green food production materials, and microbial fertilizers of lilac gray Streptomyces Hainan variant; realize the use of microbial fertilizers instead of chemical fertilizers, protect the soil environment, contribute to the food health cause of mankind, and obtain good social and economic benefits.

[0005] In order to solve the above problems, the first aspect of the present invention provides a microbial fertilizer, the components of which include the precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant, as well as a inclusion agent, an adsorbent, a filler matrix, a carbon source, a nitrogen source and an inorganic salt.

[0006] The fermentation liquid membrane filter residue after extracting the middle-stage mycotoxin from the deep fermentation liquid of Streptomyces lilacinus Hainan variant is also rich in a large number of biologically active substances necessary for plant growth, such as crude protein, polysaccharides, monosaccharides and amino acids, which are produced by the live bacteria of Streptomyces lilacinus Hainan variant and the fermentation metabolites of the culture medium. It can be mixed with natural organic carrier fillers, carbon and nitrogen sources, and inorganic salt nutrients in a certain ratio to turn waste into treasure and produce green and environmentally friendly Streptomyces lilacinus Hainan variant microbial fertilizers, providing efficient and environmentally friendly new microbial fertilizers for the production of green and healthy agricultural products.

[0007] The microbial fertilizer provided by the invention has good storage stability and the effect of promoting plant growth.

[0008] In some embodiments of the present invention, the accession number of the Streptomyces lilacinus Hainan variant is GDMCC.NO61906.

[0009] In some preferred embodiments of the present invention, the mass percentages of the precipitate, inclusion agent, adsorbent, filler matrix, carbon source, nitrogen source and inorganic salt are: 5-15%, 5-20%, 10-30%, 20-40%, 1-10%, 0.1-5% and 0.1-5%, respectively; for example, the precipitate is 10%, the inclusion agent is 20%, the adsorbent is 30%, the filler matrix is ​​34%, the carbon source is 4%, the nitrogen source is 1%, and the inorganic salt is 1%; and / or,

[0010] The inclusion agent, adsorbent, filler matrix, carbon source, nitrogen source and inorganic salt are selected from one or more of the following: the inclusion agent includes β-cyclodextrin; the adsorbent includes calcined diatomaceous earth; the filler matrix includes potassium humate; the carbon source includes brown sugar and glucose; the nitrogen source includes ammonium chloride; the inorganic salt includes sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate.

[0011] In some embodiments of the present invention, the precipitate is a solid bacterial residue retentate obtained by filtering the fermentation broth of the Streptomyces lilacinus var. Hainan through a ceramic membrane.

[0012] In some preferred embodiments of the present invention, sterile water is added to the solid bacterial residue retentate, and ultrasonic dissolution and washing are performed, followed by centrifugation to obtain the precipitate.

[0013] In some preferred embodiments of the present invention, the washing and centrifugation are repeated until the content of sinoprotinin in the supernatant is ≤50 μ / ml.

[0014] In some preferred embodiments of the present invention, the fermentation medium of the Streptomyces lilacinus Hainan variant comprises: 4.0%-15.0% glucose, 1.0%-6.0% corn starch, 0.5%-7.5% corn flour, 3.0%-12.0% soybean flour, 0.2%-1.6% sodium chloride, 0.1%-1.8% ammonium chloride, 0.2%-1.6% calcium carbonate, 0.01%-0.13% potassium dihydrogen phosphate, 0.01%-0.16% magnesium sulfate, pH 6.0-7.0; and / or,

[0015] The fermentation culture conditions of the Hainan variant of Streptomyces lilacinus are: temperature 26°C-35°C, air ventilation volume 0.5V / VM-1.5V / VM, fermentation tank stirring speed 50rpm-200rpm, fermentation culture cycle 5 days-10 days, to obtain the fermentation liquid.

[0016] In some more preferred embodiments of the present invention, the pore size of the ceramic membrane is 50 nm.

[0017] In some embodiments of the present invention, the microbial fertilizer comprises inclusion compound B, inclusion adsorption mixture G and filler matrix, wherein the mass ratio of inclusion compound B, inclusion adsorption mixture G and filler matrix is ​​(7-11):(19-29):(13.5-20.5), for example, 9:24:17;

[0018] wherein the inclusion compound B comprises B1 and B2, and the inclusion adsorption mixture G comprises G1 and G2;

[0019] Among them, the B1 is composed of the precipitate and the inclusion agent, and the B2 is composed of the carbon source, the nitrogen source, the inorganic salt and the inclusion agent; the G1 is composed of the B1 and the adsorbent, and the G2 is composed of the B2 and the adsorbent.

[0020] In some embodiments of the invention, one or more of the following conditions are met:

[0021] (1) In B1, the mass ratio of the precipitate to the inclusion agent is (0.8-1.2):(0.8-1.2), for example, 1:1;

[0022] (2) In B2, the mass ratio of the carbon source, the nitrogen source, the inorganic salt and the inclusion agent is (3.2-4.8):(0.8-1.2):(0.8-1.2):(8-12), for example, 4:1:1:10;

[0023] (3) In the B, the mass ratio of B1 to B2 is (4-5):(5-4), for example, 5:4;

[0024] (4) In G1, the mass ratio of B1 to the adsorbent is (1.6-2.4):(2.4-3.6), for example, 2:3;

[0025] (5) In G2, the mass ratio of B2 to the adsorbent is (6.4-9.6):(12-18), for example, 8:15;

[0026] (6) In the G, the mass ratio of G1 to G2 is (23-25):(25-23), for example, 25:23.

[0027] In some preferred embodiments of the present invention, in B2, the carbon source is brown sugar and glucose in a mass ratio of (2.4-3.6):(0.8-1.2), for example, 3:1; the nitrogen source is ammonium chloride; the inorganic salt is sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate in a mass ratio of (0.64-0.96):(0.08-0.12):(0.03-0.05):(0.02-0.04):(0.02-0.04), for example, 0.8:0.1:0.04:0.03:0.03.

[0028] In some more preferred embodiments of the present invention, the mass ratio of brown sugar, glucose, ammonium chloride, sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate is 3:1:1:0.8:0.1:0.04:0.03:0.03.

[0029] In some embodiments of the present invention, the precipitate contains a viable colony count of 10-20 billion CFU / g, such as 10.5 billion CFU / g, 13.5 billion CFU / g, 18.9 billion CFU / g or 14.3 billion CFU / g.

[0030] In some preferred embodiments of the present invention, the precipitate contains 25.6%-38.4% total sugar, 3.99%-5.99% reducing sugar, 18.47%-27.71% crude protein, 0.67%-1.5% amino acid in a mass ratio, for example, 32% total sugar, 4.99% reducing sugar, 23.09% crude protein, 0.83% amino acid, wherein the reducing sugar content is calculated as maltose.

[0031] In some embodiments of the present invention, the microbial fertilizer is obtained by a method comprising the following steps: mixing the precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant, a inclusion agent, an adsorbent, a filler matrix, a carbon source, a nitrogen source and an inorganic salt to obtain the microbial fertilizer.

[0032] In some preferred embodiments of the present invention, the method comprises the following steps:

[0033] (1) mixing the precipitate with the inclusion agent in proportion to obtain B1; mixing the carbon source, nitrogen source and inorganic salt with the inclusion agent in proportion to obtain B2;

[0034] (2) mixing the B1 and the adsorbent in proportion to obtain the G1; mixing the B2 and the adsorbent in proportion to obtain the G2;

[0035] (3) mixing the B1, B2, G1, G2 and / or the filler matrix in proportion to obtain the microbial fertilizer;

[0036] Wherein, in step (1), the temperature for preparing B1 is between 25°C and 30°C, and stirring is continued for 60 minutes; the material temperature for preparing B2 is 50°C to 60°C, the feed speed is 850rpm to 1200rpm, and the classification speed is 620rpm to 680rpm;

[0037] In step (2), the temperature for preparing G1 is maintained at 25°C-30°C; the material temperature for preparing G2 is 50°C-60°C, the feed speed is 800rpm-1000rpm, and the classification speed is 600rpm-650rpm;

[0038] In step (3), the process comprises first mixing the B and G in proportion to obtain a mixture BG, and then mixing the mixture with a filler matrix in proportion to obtain the microbial fertilizer.

[0039] In order to solve the above technical problems, the second aspect of the present invention provides a method for preparing the microbial fertilizer according to the first aspect of the present invention, the preparation method comprising the following steps:

[0040] The microbial fertilizer is obtained by mixing the precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant, the inclusion agent, the adsorbent, the filler matrix, the carbon source, the nitrogen source and the inorganic salt.

[0041] In some embodiments of the present invention, the preparation method comprises the following steps:

[0042] (1) mixing the precipitate with the inclusion agent in proportion to obtain B1; mixing the carbon source, nitrogen source and inorganic salt with the inclusion agent in proportion to obtain B2;

[0043] (2) mixing the B1 and the adsorbent in proportion to obtain the G1; mixing the B2 and the adsorbent in proportion to obtain the G2;

[0044] (3) mixing the B1, B2, G1, G2 and / or the filler matrix in proportion to obtain the microbial fertilizer;

[0045] Wherein, in step (1), the temperature for preparing B1 is between 25°C and 30°C, and stirring is continued for 60 minutes; the material temperature for preparing B2 is 50°C to 60°C, the feed speed is 850rpm to 1200rpm, and the classification speed is 620rpm to 680rpm;

[0046] In step (2), the temperature for preparing G1 is maintained at 25°C-30°C; the material temperature for preparing G2 is 50°C-60°C, the feed speed is 800rpm-1000rpm, and the classification speed is 600rpm-650rpm;

[0047] In step (3), the process comprises first mixing the B and G in proportion to obtain a mixture BG, and then mixing the mixture with a filler matrix in proportion to obtain the microbial fertilizer.

[0048] In order to solve the above technical problems, the third aspect of the present invention provides a use of the microbial fertilizer as described in the first aspect of the present invention in increasing crop yield.

[0049] On the basis of being in accordance with the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0050] The reagents and raw materials used in the present invention are commercially available.

[0051] The positive and progressive effects of the present invention are:

[0052] 1. The precipitate of the fermentation liquid of the Hainan variant of Streptomyces lilacinus, as well as nutrients such as inclusion agents, adsorbents, filler matrices, carbon sources, nitrogen sources and inorganic salts are adsorbed, included and mixed in a certain ratio and sequence to produce green microbial fertilizer, so as to turn waste into treasure and achieve environmentally friendly production compatibility.

[0053] 2. In the process of producing green and environmentally friendly biological pesticides such as streptomyces lilacinus, the green agricultural material, the Hainan variant of Streptomyces lilacinus, is produced simultaneously, forming a complete clean production industry chain for the production of green agricultural materials using the Hainan variant of Streptomyces lilacinus.

[0054] 3. The microbial fertilizer of the present invention has a unique formula. In the preparation process, a special process of adsorption and inclusion is applied, which is conducive to the gradual slow release of nutrients such as carbon and nitrogen sources and inorganic salts, and is conducive to the gradual uptake and utilization of nutrients required for survival by the viable bacteria of the Hainan variant of Streptomyces lilacinus in the microbial fertilizer, and is conducive to the long-term preservation and growth of the viable bacteria of the Hainan variant of Streptomyces lilacinus under a high humidity state with a moisture content of ≥5% and ≤35%. Under cool, dry and ventilated conditions, after the microbial fertilizer is preserved for 12 months, the effective viable bacteria count of the Hainan variant of Streptomyces lilacinus is still ≥200 million CFU / g, the moisture content is ≤35%, and the fineness is ≥80%;

[0055] 4. The calcined diatomaceous earth and mineral potassium humate fillers used in the microbial fertilizer of the present invention make the microbial fertilizer porous and rich in nutrition, which is beneficial to the storage, recovery, growth and reproduction of the live bacteria of the Hainan variant of Streptomyces lilacinus, forming a pH value suitable for the growth and reproduction of beneficial bacteria, forming a dominant bacterial community, and inhibiting the breeding and proliferation of pathogenic bacteria; forming a good superposition of the respective advantages of the Hainan variant of Streptomyces lilacinus and the calcined diatomaceous earth and mineral potassium humate, which is more conducive to improving soil properties, increasing soil aggregates and air permeability, and is beneficial to plant growth and reducing diseases, achieving the effect of reducing medicine, weight loss, increasing production and increasing income during plant growth.

[0056] 5. The microbial fertilizer process of the present invention is simple, easy to operate, does not require secondary stacking fermentation and drying processes, and fully utilizes the membrane filtration wet residues produced in the production of streptomyces lilacinus Hainan variant in the process of producing streptomyces for complete green recycling, achieving consumption reduction and emission reduction, circular economy, and clean production, with good economic and social benefits. DETAILED DESCRIPTION

[0057] For the purpose of the present invention, unless otherwise specified, the terms used in this application have the following meanings:

[0058] The term "microbial fertilizer" refers to a product containing specific living microorganisms, which is used in agricultural production to increase the supply of plant nutrients or promote plant growth through the life activities of the microorganisms contained therein, thereby increasing yields and improving the quality of agricultural products and the agricultural ecological environment. Currently, microbial fertilizers include microbial inoculants (microbial agents), compound microbial fertilizers and bio-organic fertilizers.

[0059] Among them, microbial inoculants (microbial agents) refer to live bacterial products made by directly using one or more target microorganisms after industrial production and proliferation, or by concentrating, drying or adsorbing them on a carrier.

[0060] The term "CFU" (colony forming units) refers to colony forming units, which refers to each colony formed after incubation at a certain temperature and time on an agar plate.

[0061] The specific technical solution adopted by the present invention is as follows:

[0062] The present invention is further described in detail below in conjunction with specific implementation modes.

[0063] 1. The present invention discloses a bacterial agent composed of a Hainan variant of Streptomyces lilacinus and other fillers, as well as a preparation method and application. The formula is unique, and the live bacteria and fillers have a strong synergistic and reinforcing function with each other, which is beneficial to promoting the storage and growth and reproduction of the live bacteria of the Hainan variant of Streptomyces lilacinus, forming a dominant bacterial community, inhibiting the growth and value-added of pathogenic bacteria, improving soil properties, increasing soil aggregates and air permeability, and forming a suitable pH value suitable for the growth and reproduction of the beneficial bacteria, which is beneficial to plant growth and reduces diseases. The formula composition includes: live bacteria of the Hainan variant of Streptomyces lilacinus and metabolites of the fermentation medium, carbon and nitrogen sources required for microbial growth, inorganic salt nutrients and fillers of the bacterial agent. Among them:

[0064] 1.1. Live cells of Streptomyces lilacinus Hainan variant and metabolites of fermentation medium:

[0065] 1.1.1. The viable bacteria of Streptomyces lilacinus Hainan variant are pure viable bacteria in the wet membrane filter residue obtained by fermenting the fermentation liquid of Streptomyces lilacinus Hainan variant to produce the secondary metabolite of Zhongshengmycin, and then filtering the fermentation liquid through a membrane to separate the liquid and solid, and the activity of the pure viable bacteria is ≥ 10 billion CFU / g;

[0066] 1.1.2. The metabolites of fermentation medium are the secondary metabolites of Streptomyces lilacinus Hainan variant fermentation. The wet membrane residue after the fermentation liquid is filtered through the membrane for liquid-solid separation is rich in crude protein, polysaccharides, monosaccharides, amino acids and other active nutrients that promote plant growth.

[0067] 1.2. Filler of bacterial agent:

[0068] 1.2.1. Potassium humate, a mineral source rich in potassium, is preferably used as the filler matrix of the bacterial agent:

[0069] 1.2.1.1. Mineral potassium humate is a pure natural mineral active potassium fertilizer, containing trace elements, rare earth elements, plant growth regulators, virus inhibitors and other nutrients. It is a small molecule organic matter with a general content of 50-60% organic matter, 70-80% humic acid, 8-12% potassium oxide, and a pH value of 7-9. As a filler for bacterial agents, it makes nutrients more sufficient and replenishment more reasonable, avoids the occurrence of various physiological diseases of crops caused by lack of elements, makes crops more vigorous, leaves more dark green, more resistant to lodging and is natural and environmentally friendly.

[0070] 1.2.1.2. Mineral potassium humate is rich in functions and has strong adsorption and ion exchange capabilities. It can exchange and adsorb soil salt ions and has a high soil buffering capacity for harmful salts, which is usually 10-20 times larger than that of ordinary soils. It promotes the formation of soil aggregate structure and flocculates with soil particle structure to form a stable small fertilizer reservoir that retains water, fertilizer and air permeability for use by crops when needed.

[0071] 1.2.1.3. Mineral potassium humate has the ability to chelate trace elements such as calcium, magnesium, zinc, and molybdenum in the soil to generate highly soluble and easily absorbed humic acid calcium and zinc. It can also chelate with the soil's solidified phosphorus and solidified potassium to naturally regulate the activity and content of nutrients in the soil, effectively improving nutrient utilization, enhancing disease resistance and stress resistance, increasing plant yields, and improving quality.

[0072] 1.2.1.4. Mineral potassium humate has good water solubility and is easy to prepare and use for microbial agents: it can be used for drip irrigation and flushing, which is beneficial to improving photosynthesis and regulating respiration, improving crop metabolism, promoting root growth, tree growth, flowering and fruiting, and reducing or alleviating crop physiological damage.

[0073] 1.2.1.5. Mineral potassium humate has a small number of miscellaneous bacteria and does not bring in harmful pathogenic bacteria, insect eggs and other pathogenic factors. It is used as a filler for the live bacteria of the Hainan variant of Streptomyces lilacinus. It is beneficial to promote the growth and reproduction of the live bacteria of the Hainan variant of Streptomyces lilacinus, form a dominant bacterial community, inhibit the growth and increase of pathogenic bacteria, improve soil properties, increase soil aggregates and permeability, and form a suitable pH value suitable for the growth and reproduction of the beneficial bacteria, which is beneficial to plant growth and reduces diseases.

[0074] 1.2.2. Calcined diatomaceous earth is preferably used as the adsorbent of the bacterial agent:

[0075] 1.2.2.1. Calcined diatomaceous earth has a neutral pH value and is non-toxic. Its water content is ≤3%-5%. Its moisture absorption rate is 4-6 times of its volume. It has a light bulk density and a fineness evenly distributed between 325 mesh and 500 mesh. It has good suspension performance, a porous structure and strong adsorption performance. It contains a small number of miscellaneous bacteria and does not bring in harmful pathogenic bacteria, insect eggs and other pathogenic factors. It will not clog agricultural machinery pipelines when used. It can moisturize and loosen the soil in the soil, prolong the efficacy of drugs and fertilizers, and promote the growth of crops. It is widely used as a filler adjuvant for biological pesticide wettable powders, dryland herbicides, and paddy field herbicides.

[0076] 1.2.2.2. Calcined diatomite density 1.9-2.3g / cm 3 , bulk density 0.34-0.65g / cm 3 , with a specific surface area of ​​40-65㎡ / g. Under an electron microscope, it can be observed that the shell has a number of special porous pore structures, with a porosity of 90-92% and a pore volume of 0.45-0.98cm 3 / g, it has strong adsorption and moisture absorption capabilities and is widely used in compound fertilizers for various crops such as fruit trees, vegetables, flowers and plants. It has become an efficient fertilizer for promoting crop growth and improving soil.

[0077] 1.2.3. β-cyclodextrin is preferably used as the inclusion agent of the bacterial agent:

[0078] 1.2.3.1. The β-cyclodextrin molecule has a special ring structure, making it an inclusion material with wide application value: there is a hole in the center of the ring structure, and the interior is sulfur-water-based. It can complex other substances including rare gases, halogens, dyes, fragrances, drugs, microorganisms, foods, pesticides and preservatives into inclusion compounds through weak van der Waals forces. The stability, volatility, solubility and reactivity of other substances after inclusion are greatly improved.

[0079] 1.2.3.2. The β-cyclodextrin contains few foreign bacteria and does not bring in harmful pathogenic bacteria, insect eggs and other pathogenic factors. It is widely used in the pesticide and fertilizer industries: some pesticides and fertilizers are stabilized by cyclodextrin inclusion, which greatly improves the stability and storage capacity of the pesticides and fertilizers, enhances the insecticidal and bactericidal effects, and at the same time allows the drug and fertilizer effects to be slowly released to prolong the drug efficacy against insect pests and fungal pests, prolong the fertilizer efficacy on plants, and reduce the irritation and toxicity of drugs and fertilizers to humans, animals and plants.

[0080] 1.3. Carbon and nitrogen sources and inorganic salt nutrients required for microbial growth:

[0081] 1.3.1. Use brown sugar, glucose and ammonium chloride as carbon and nitrogen sources to enable the viable bacteria of Streptomyces lilacinus Hainan variant contained in the bacterial agent to quickly obtain the carbon and nitrogen sources required for recovery, growth and reproduction when the bacterial agent is used;

[0082] 1.3.2. Sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate are inorganic salt nutrients required for inducing recovery, growth and reproduction of the live bacteria of the Hainan variant of Streptomyces lilacinus when the bacterial agent is used.

[0083] 1.3.3. The carbon and nitrogen sources and inorganic salt nutrients contain a small number of miscellaneous bacteria and do not bring in harmful pathogenic bacteria, insect eggs and other pathogenic factors, which is conducive to the preservation and production and reproduction of the live bacteria of the Hainan variant of the bacterial agent Streptomyces lilacinus.

[0084] 1.4. A microbial fertilizer formula composed of a Hainan strain of Streptomyces lilacinus and other fillers (mass percentage):

[0085] 1.4.1. Live cells of Streptomyces lilacinus strain Hainan and metabolites of fermentation medium (wet weight): 10%;

[0086] 1.4.2. Filler of bacterial agent: 84%, including:

[0087] 1.4.2.1. Mineral potassium humate: 34%;

[0088] 1.4.2.2. Calcined diatomaceous earth: 30%;

[0089] 1.4.2.3.β-cyclodextrin: 20%.

[0090] 1.4.3. Carbon and nitrogen sources and inorganic salt nutrients required for microbial growth: 6%, including:

[0091] 1.4.3.1. Sucrose 3%;

[0092] 1.4.3.2. Glucose 1%;

[0093] 1.4.3.3. Ammonium chloride 1%;

[0094] 1.4.3.4. Sodium chloride 0.8%;

[0095] 1.4.3.5. Manganese sulfate 0.1%;

[0096] 1.4.3.6. Light calcium carbonate 0.04%;

[0097] 1.4.3.7. Magnesium sulfate 0.03%;

[0098] 1.4.3.8. Potassium dihydrogen phosphate 0.03%.

[0099] 2. A microbial fertilizer composed of a Hainan variant of Streptomyces lilacinus and other fillers, and a preparation method and application thereof, wherein the preparation method comprises:

[0100] 2.1. Obtaining live cells of Streptomyces lilacinus Hainan variant and metabolites of fermentation medium;

[0101] 2.2. Inclusion of live cells of Streptomyces lilacinus var. Hainan and metabolites of fermentation medium with β-cyclodextrin (inclusion complex B1 of the first-order adsorption inclusion of live cells);

[0102] 2.3. Inclusion of carbon and nitrogen sources, inorganic salt nutrients and β-cyclodextrin (inclusion complex B2 of the first-order adsorption inclusion of nutrients);

[0103] 2.4. Fully mix 50% of the live bacteria inclusion complex B1 and 50% of the nutrient inclusion complex B2 to obtain a mixture B of live bacteria and nutrients in the first order of adsorption inclusion, ensuring that the nutrients required for the growth and survival of the live bacteria are met;

[0104] 2.5. The remaining 50% of the living cell inclusion complex B1 and 50% of the calcined diatomaceous earth were fully adsorbed and mixed uniformly to obtain the adsorption inclusion complex G1;

[0105] 2.6. The remaining 50% of the nutrient inclusion complex B2 and the remaining 50% of the calcined diatomaceous earth are fully adsorbed and mixed uniformly to obtain an adsorption inclusion complex G2;

[0106] 2.7. Fully adsorb and mix the bacterial adsorption inclusion compound G1 and the nutrient adsorption inclusion compound G2 to obtain an inclusion adsorption mixture G;

[0107] 2.8. The inclusion mixture B and the inclusion adsorption mixture G are fully mixed to obtain a microenvironment with binary inclusion adsorption of live bacteria and gradual release of nutrients, which is conducive to the slow balance of live bacteria during storage to obtain the gradual gradient release of the nutrient mixture BG required for metabolism;

[0108] 2.9. The mixture BG is fully mixed with the mineral potassium humate to obtain a bacterial agent having live cells of Streptomyces lilacinus Hainan variant and metabolites of the fermentation medium.

[0109] The following is a microbial fertilizer composed of a Hainan variant of Streptomyces lilacinus and other fillers, as well as a preparation method and application thereof, in conjunction with the specific operation method of the present invention. The provided embodiments are only used to further illustrate the present invention and do not constitute any limitation to the scope of the present invention.

[0110] The experimental methods in the following examples without specifying specific conditions were carried out according to conventional methods and conditions, or selected according to the product instructions.

[0111] Example 1 Obtaining the centrifugal precipitate (rich in viable cells of Streptomyces lilacinus var. Hainan and fermentation medium metabolites) from the fermentation liquid membrane filtration of wet bacterial residues

[0112] 10 kg of fermentation liquid (pH 7.0 ± 0.5) of Streptomyces lilacinus Hainan variant was taken and filtered through a ceramic membrane. The permeate of the ceramic membrane was used to extract the secondary metabolite biological pesticide zhongshengmycin. The residue retained by the ceramic membrane was added with an equal amount of sterile water, mixed with ultrasonic to fully dissolve and wash, and then centrifuged at 3500 rpm for 15 min. The precipitate obtained by centrifugation was mixed with an equal amount of sterile water again, ultrasonically dissolved and washed, and then centrifuged at 3500 rpm for 15 min. This was repeated 5 times until the zhongshengmycin content of the centrifugal supernatant was ≤50 μ / ml. Finally, the centrifugal precipitate of the wet bacterial residue filtered by the fermentation liquid of Streptomyces lilacinus Hainan variant was obtained, and the colony count was determined to be 1.43×10 10 The precipitate was made into a dry powder with a water content of about 3%, and the metabolites in the dry powder were measured. The total sugar content was 32% by mass, the reducing sugar (calculated as maltose) content was 4.99%, the crude protein content was 23.09%, and the amino acid content was 0.83%.

[0113] Example 2 Preparation of live bacterial inclusion complex (live bacterial inclusion complex B1) of centrifugal sediment from fermentation liquid membrane filtration of Streptomyces lilacinus variant Hainan and filler β-cyclodextrin

[0114] Prepared by wet inclusion method:

[0115] Take 10 kg of fermentation broth membrane filtration centrifugal precipitate of Streptomyces lilacinus Hainan variant with a moisture content of about 75%-85% and 10 kg of β-cyclodextrin, put 10 kg of fermentation broth membrane filtration centrifugal precipitate into a horizontal double-shaft paddle mixer with a cooling jacket, first start the cooling water system of the mixer jacket, then start stirring, and control the stirring speed to 50 rpm / min; add 2 kg of β-cyclodextrin every 10 minutes, and add 10 kg of β-cyclodextrin in total for 5 times; maintain the stirring adsorption inclusion temperature between 25°C and 30°C, and continue stirring for 60 minutes, so that the live bacteria and metabolites in the precipitate can enter the hole space in the center of the cyclodextrin ring structure for adsorption, attachment and inclusion, and avoid overheating to cause the live bacteria to die, and obtain 20 kg of a moist primary wet adsorption live bacteria inclusion complex (B1).

[0116] Example 3 Preparation of nutrient inclusion complex of carbon and nitrogen source, inorganic salt nutrient and filler β-cyclodextrin (nutrient inclusion complex B2)

[0117] Prepared by dry inclusion method:

[0118] Take carbon and nitrogen sources, inorganic salt nutrients, 3kg brown sugar, 1kg glucose, 1kg ammonium chloride, 0.8kg sodium chloride, 0.1kg manganese sulfate, 0.04kg light calcium carbonate, 0.03kg magnesium sulfate, 0.03kg potassium dihydrogen phosphate, and 10kg β-cyclodextrin.

[0119] First, add 10 kg of β-cyclodextrin into the stirring and shearing mixer and start stirring; add one material every 5 minutes, and the order of addition is potassium dihydrogen phosphate, magnesium sulfate, light calcium carbonate, manganese sulfate, sodium chloride, ammonium chloride, glucose, and brown sugar; keep the material temperature between 50°C and 60°C during the stirring and shearing mixing process, and continue shearing and stirring for 30 minutes after the feeding is completed to ensure full and uniform mixing.

[0120] The above-mentioned uniformly mixed mixture is mixed by ultrafine grinding and the material temperature is maintained at a relatively high temperature of 50°C-60°C so that the carbon and nitrogen sources and inorganic salt nutrients are included and adsorbed by cyclodextrin. The ultrafine grinding machine feed speed is controlled to be 850rpm-1200rpm, and the classification speed is 620rpm-680rpm to obtain a bulk density of 0.48g / cm 3 -0.58g / cm 3 16kg of powdered primary dry adsorption nutrient inclusion compound (B2).

[0121] Example 4 Preparation of adsorption inclusion complex (inclusion complex B) of viable bacteria of Streptomyces lilacinus var. Hainan and carbon and nitrogen sources and inorganic salt nutrients

[0122] 10 kg of the first-level wet adsorption inclusion compound (B1) is put into a horizontal double-shaft paddle mixer with a cooling jacket, and after stirring for 10 minutes, 8 kg of the first-level dry adsorption inclusion compound (B2) is added; the stirring and adsorption inclusion temperature is maintained between 25°C and 30°C, and stirring is continued for 60 minutes to obtain 18 kg of a second-level mixed adsorption inclusion compound (B) of the viable bacteria of Streptomyces lilacinus Hainan variant and carbon and nitrogen sources and inorganic salt nutrients. The moisture content of the mixture of the viable bacteria inclusion compound and the nutrient inclusion compound is reduced, which is conducive to the viable bacteria to slowly absorb the slowly released nutrients and enhance the air permeability of the viable bacteria.

[0123] Example 5 Preparation of adsorption mixture (G1) of viable bacterial inclusion complex of Streptomyces lilacinus var. Hainan and diatomaceous earth

[0124] First, 15 kg of calcined diatomaceous earth was added into a horizontal twin-shaft paddle mixer with a cooling jacket. After stirring for 10 minutes, 10 kg of inclusion compound B1 was added at a rate of 2 kg every 10 minutes and mixed. After all B1 was added, stirring and mixing were continued for 60 minutes. The temperature was maintained at 25°C-30°C during the entire stirring and mixing process to obtain 25 kg of an adsorption mixture (mixture G1) of live bacteria inclusion compound and diatomaceous earth that further reduced moisture content and was beneficial to the respiration of live bacteria.

[0125] Example 6 Preparation of adsorption mixture of nutrient inclusion compound and diatomaceous earth (G2)

[0126] First, 15 kg of calcined diatomite was added into a stirring and shearing mixer. After stirring for 10 minutes, 8 kg of the above-mentioned primary dry adsorption inclusion compound (B2) was added once every 10 minutes at a rate of 2 kg each time for sufficient crushing, mixing and stirring. The material temperature during the stirring and shearing mixing process was maintained between 50° C. and 60° C., and the shearing and stirring were continued for 30 minutes to make it fully mixed. The mixture of the diatomite and the primary dry adsorption inclusion compound was mixed by ultrafine grinding and maintained at a relatively high temperature of 50° C. to facilitate further inclusion and adsorption of the diatomite and the primary dry adsorption inclusion compound. The feeding speed of the ultrafine grinder was controlled to be 800 rpm-1000 rpm, and the classification speed was 600 rpm-650 rpm to obtain a bulk density of 0.40 g / cm 3 -0.55g / cm 3 23 kg of the powdery dry adsorption mixture (mixture G2).

[0127] Example 7 Preparation of a mixture (G) containing live cells of Streptomyces lilacinus, Hainan variant, nutrients and diatomaceous earth inclusion adsorption

[0128] A total of 25 kg of an adsorption mixture (G1) of a live bacterial cell inclusion complex of the Hainan variant of Streptomyces lilacinus and diatomaceous earth is first put into a horizontal double-shaft paddle mixer with a cooling jacket, and after stirring for 10 minutes, a total of 23 kg of an adsorption mixture (G2) of a nutrient inclusion complex and diatomaceous earth is added; the temperature of the stirring and mixing process is maintained between 25° C. and 30° C., and stirring is continued for 60 minutes to obtain 48 kg of an adsorption mixture (G) rich in live bacterial cells, nutrients, and diatomaceous earth, wherein the moisture content of the mixture (G) is further reduced, and the air permeability of the live bacterial cells and the ability of the nutrients to gradually release are further enhanced.

[0129] Example 8 Preparation of Nutrient Adsorption Inclusion Compound Mixture (BG) of Live Cells of Streptomyces lilacinus var. Hainan

[0130] A total of 48 kg of the viable thalli of Streptomyces hainanensis, nutrients, and diatomaceous earth inclusion adsorption mixture (G) were first put into a horizontal double-shaft paddle mixer with a cooling jacket, and stirred for 10 minutes. Then, a total of 18 kg of the adsorption inclusion compound (inclusion compound B) of the viable thalli of Streptomyces hainanensis, carbon and nitrogen sources, and inorganic salt nutrients were added at intervals of 10 minutes, 6 kg each time, and fully stirred and mixed. After all were added, stirring and mixing was continued for 60 minutes. The entire stirring and mixing process was maintained at 25°C-30°C to obtain 66 kg of the adsorption inclusion compound mixture (mixture BG) of the viable thalli of Streptomyces hainanensis. The moisture content, viable bacteria count, and nutrients of the mixture BG were relatively uniform, the air permeability was relatively good, and the carbon and nitrogen sources and inorganic salt nutrients had a relative slow release capacity.

[0131] Example 9 Preparation of bacterial agent

[0132] A total of 66 kg of a nutrient adsorption inclusion compound mixture (BG) of a live bacterial cell of Streptomyces lilacinus Hainan variant with a sustained-release ability is first put into a horizontal double-shaft paddle mixer with a cooling jacket, and after stirring for 10 minutes, 34 kg of mineral potassium humate is added at once and fully stirred and mixed. The entire stirring and mixing process is maintained at 25° C.-30° C., and the stirring is maintained for 60 minutes to obtain 100 kg of a bacterial agent. The nutrient of the bacterial agent has a sustained-release ability, which is beneficial to the preservation and recovery of the live bacterial cell of Streptomyces lilacinus Hainan variant. It is determined that the bacterial agent contains 920 million CFU / g of the colony of Streptomyces lilacinus Hainan variant, 21% of moisture, 90% of fineness, and pH 7.8.

[0133] Example 10 Bacterial Agent Shelf Stability Test

[0134] The bacterial agent obtained in Example 9 was vacuum packed in aluminum-plastic bags, with 1000 g per bag.

[0135] Take 10 kg of the membrane filtration centrifugal precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant with a moisture content of about 75%-85%, 45 kg of calcined diatomaceous earth, and 45 kg of mineral potassium humate, mix them evenly at room temperature to obtain No. 1 bacterial agent, and use aluminum-plastic vacuum packaging to pack the bacterial agent, with 1000 g per bag.

[0136] Take 10kg of the fermentation liquid membrane filtration centrifugal precipitate of the Streptomyces lilacinus Hainan variant with a moisture content of about 75%-85%, 20kg of β-cyclodextrin, 30kg of calcined diatomaceous earth, 6kg of the carbon and nitrogen source, inorganic salt nutrients (3kg of brown sugar, 1kg of glucose, 1kg of ammonium chloride, 0.8kg of sodium chloride, 0.1kg of manganese sulfate, 0.04kg of light calcium carbonate, 0.03kg of magnesium sulfate, 0.03kg of potassium dihydrogen phosphate), and 34kg of potassium humate, mix them evenly at room temperature to obtain No. 2 bacterial agent, and use aluminum-plastic vacuum packaging to package the bacterial agent in 1000g bags.

[0137] The bacterial agent obtained in Example 9, bacterial agent No. 1 and bacterial agent No. 2 were stored under the conditions of temperature ≤ 20°C, relative humidity ≤ 65%, and no direct sunlight, and data detection was performed every 3 months for a period of 12 months. The colony count, pH value, fineness and moisture content of Streptomyces lilacinus variant Hainan are shown in Table 1:

[0138] Table 1 Shelf stability data of bacterial agents

[0139]

[0140]

[0141] The results show that the bacterial agent obtained in Example 9 is stored under cool, dry and ventilated conditions for 12 months, with an effective viable count of ≥200 million CFU / g, a moisture content of ≤35%, a fineness of ≥80%, and a pH of 5.5-8.5. The effective viable counts in bacterial agent No. 2 are all lower than 200 million CFU / g, and bacterial agent No. 1 is even lower than 150 million CFU / g. It can be seen that the bacterial agent of Example 9 has a unique formula and is obtained through a special process of adsorption and inclusion, which is conducive to preservation.

[0142] Example 11: Experimental application of bacterial agent in the cultivation of sweet potatoes in mountainous areas

[0143] Select the Xiangyang Mountain in Changtai, southern Fujian, to carry out sweet potato cultivation test verification with the bacterial agent of Example 9 of the present invention, bacterial agent No. 1, bacterial agent No. 2 and blank control, using 8 plots, each plot is 3.5 meters × 6.5 meters. In the clear weather in early August, the 8 test plots were plowed to a depth of 40cm-45cm, exposed to the sun for 3 days, and plowed once a day; the last time, the longitudinal direction of the plot was plowed every 50cm to form a ridge about 40cm-45cm high, and a sweet potato seedling was cultivated every 50cm on the ridge. The 8 plots were divided into two rows in the east-west direction, and 4 plots in each row were cultivated. The front row plots are arranged according to the serial number plots: ① No. 1 bacterial agent, ② No. 2 bacterial agent, ③ Example 9 bacterial agent, ④ blank control; the back row plots are arranged according to the serial number plots in the same direction of the front row: ⑤ blank control, ⑥ Example 9 bacterial agent, ⑦ No. 2 bacterial agent, ⑧ No. 1 bacterial agent to cultivate sweet potatoes.

[0144] Take one package each of bacterial agent No. 1, bacterial agent No. 2, and bacterial agent of Example 9 in aluminum-plastic vacuum packaging that have been stored for 6 months under the conditions of temperature ≤ 20° C., relative humidity ≤ 65%, and no direct sunlight.

[0145] Take 0.1kg of No. 1 bacterial agent and mix it evenly with 10kg of fine soil after exposure to the sun; take 0.1kg of No. 2 bacterial agent and mix it evenly with 10kg of fine soil after exposure to the sun; take 0.1kg of Example 9 bacterial agent and mix it evenly with 10kg of fine soil after exposure to the sun.

[0146] The 8 plots were all dug with holes at intervals of 50 cm and a depth of 30 cm; a uniform mixture of 120 g of fine soil and No. 1 bacterial agent was added to the bottom layer of the holes in plots ①⑧; a uniform mixture of 120 g of fine soil and No. 2 bacterial agent was added to the bottom layer of the holes in plots ②⑦; a uniform mixture of 120 g of fine soil and Example 9 bacterial agent was added to the bottom layer of the holes in plots ③⑥; a uniform mixture of 120 g of fine soil and a blank control substance was added to the bottom layer of the holes in plots ④⑤; the surface layer of each of the above-mentioned holes was covered with 10 cm-15 cm of fine soil, and the surface of the fine soil was covered with sunshade dead branches and dry grass to facilitate the recovery of live bacteria, and sweet potato seedlings were cultivated according to conventional cultivation methods in the evening of the next day; subsequent daily management was carried out according to routine.

[0147] The sweet potatoes were harvested uniformly after 3 and a half months of cultivation. Taking the blank control as 100%, the average yield is shown in Table 2.

[0148] Table 2 Data on the application of microbial agents in the cultivation of sweet potatoes in mountainous areas

[0149]

[0150] It can be seen from the above results that compared with the blank control, the No. 1 bacterial agent has the worst yield-increasing effect, with an increase of less than 5%, the No. 2 bacterial agent has a better yield-increasing effect, with an increase of 10.80%, and the bacterial agent obtained in Example 9 has the best effect, with an increase of 27.03%. It can be seen that the No. 2 bacterial agent and the Example 9 bacterial agent have unique formulas and can effectively increase the yield of crops, especially the Example 9 bacterial agent, which is obtained by a special process of adsorption and inclusion. It is beneficial to the gradual slow release of nutrients such as carbon and nitrogen sources and inorganic salts during use, and is beneficial to the gradual absorption and utilization of nutrients required for survival by the live bacteria of the Hainan variant of Streptomyces lilacinus in the bacterial agent. The fertilizer effect is long-term and stable, and can continuously supply the plant's demand for nutrients during the entire production period, which can better promote the growth of crops.

[0151] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of implementation of the present invention. Therefore, any changes or modifications made according to the claims and description of the present invention should fall within the scope of the patent of the present invention.

Claims

1. A microbial fertilizer, characterized in that: The components of the microbial fertilizer include the precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant, as well as a complexing agent, an adsorbent, a filler matrix, a carbon source, a nitrogen source and an inorganic salt.

2. The microbial fertilizer according to claim 1, characterized in that The deposit number of the Streptomyces lilacinus Hainan variant is GDMCC.NO 61906; Preferably, the mass percentages of the precipitate, inclusion agent, adsorbent, filler matrix, carbon source, nitrogen source and inorganic salt are 5-15%, 5-20%, 10-30%, 20-40%, 1-10%, 0.1-5% and 0.1-5%, respectively; and / or, The inclusion agent, adsorbent, filler matrix, carbon source, nitrogen source and inorganic salt are selected from one or more of the following: the inclusion agent includes β-cyclodextrin; the adsorbent includes calcined diatomaceous earth; the filler matrix includes potassium humate; the carbon source includes brown sugar and glucose; the nitrogen source includes ammonium chloride; the inorganic salt includes sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate.

3. The microbial fertilizer according to claim 1 or 2, characterized in that The precipitate is a solid fungus residue intercept obtained by filtering the fermentation liquid of the Streptomyces lilacinus Hainan variant through a ceramic membrane; Preferably, the fermentation medium of the Streptomyces lilacinus Hainan variant comprises: 4.0%-15.0% glucose, 1.0%-6.0% corn starch, 0.5%-7.5% corn flour, 3.0%-12.0% soybean flour, 0.2%-1.6% sodium chloride, 0.1%-1.8% ammonium chloride, 0.2%-1.6% calcium carbonate, 0.01%-0.13% potassium dihydrogen phosphate, 0.01%-0.16% magnesium sulfate, pH 6.0-7.0; and / or, The fermentation culture conditions of the Hainan variant of Streptomyces lilacinus are: temperature 26°C-35°C, air ventilation volume 0.5V / VM-1.5V / VM, fermentation tank stirring speed 50rpm-200rpm, fermentation culture cycle 5 days-10 days, to obtain the fermentation liquid.

4. The microbial fertilizer according to any one of claims 1 to 2, characterized in that The microbial fertilizer comprises inclusion compound B, inclusion adsorption mixture G and filler matrix, wherein the mass ratio of inclusion compound B, inclusion adsorption mixture G and filler matrix is ​​(7-11):(19-29):(13.5-20.5); wherein the inclusion compound B comprises B1 and B2, and the inclusion adsorption mixture G comprises G1 and G2; Among them, the B1 is composed of the precipitate and the inclusion agent, and the B2 is composed of the carbon source, the nitrogen source, the inorganic salt and the inclusion agent; the G1 is composed of the B1 and the adsorbent, and the G2 is composed of the B2 and the adsorbent.

5. The microbial fertilizer according to claim 4, characterized in that It meets one or more of the following conditions: (1) In B1, the mass ratio of the precipitate to the inclusion agent is (0.8-1.2):(0.8-1.2), for example, 1:1; (2) In B2, the mass ratio of the carbon source, the nitrogen source, the inorganic salt and the inclusion agent is (3.2-4.8):(0.8-1.2):(0.8-1.2):(8-12), for example, 4:1:1:10; (3) In the B, the mass ratio of B1 to B2 is (4-5):(5-4), for example, 5:4; (4) In G1, the mass ratio of B1 to the adsorbent is (1.6-2.4):(2.4-3.6), for example, 2:3; (5) In G2, the mass ratio of B2 to the adsorbent is (6.4-9.6):(12-18), for example, 8:15; (6) In the G, the mass ratio of G1 to G2 is (23-25):(25-23), for example, 25:23; Preferably, in B2, the carbon source is brown sugar and glucose in a mass ratio of (2.4-3.6):(0.8-1.2), for example 3:1; the nitrogen source is ammonium chloride; the inorganic salt is sodium chloride, potassium dihydrogen phosphate, light calcium carbonate, magnesium sulfate and manganese sulfate in a mass ratio of (0.64-0.96):(0.08-0.12):(0.03-0.05):(0.02-0.04):(0.02-0.04).

6. The microbial fertilizer according to any one of claims 1 to 5, characterized in that The precipitate contains 10-20 billion CFU / g of viable bacterial colonies; Preferably, the precipitate comprises, by mass content, 25.6%-38.4% total sugars, 3.99%-5.99% reducing sugars, 18.47%-27.71% crude protein, and 0.67%-1.5% amino acids, for example, 32% total sugars, 4.99% reducing sugars, 23.09% crude protein, and 0.83% amino acids.

7. The microbial fertilizer according to any one of claims 1 to 6, characterized in that The microbial fertilizer is obtained by a method comprising the following steps: mixing the precipitate of the fermentation liquid of Streptomyces lilacinus var. Hainan, a clathrate, an adsorbent, a filler matrix, a carbon source, a nitrogen source and an inorganic salt to obtain the microbial fertilizer; Preferably, the method comprises the following steps: (1) mixing the precipitate with the inclusion agent in proportion to obtain B1; mixing the carbon source, nitrogen source and inorganic salt with the inclusion agent in proportion to obtain B2; (2) mixing the B1 and the adsorbent in proportion to obtain the G1; mixing the B2 and the adsorbent in proportion to obtain the G2; (3) mixing the B1, B2, G1, G2 and / or the filler matrix in proportion to obtain the microbial fertilizer; Wherein, in step (1), the temperature for preparing B1 is between 25°C and 30°C, and stirring is continued for 60 minutes; the material temperature for preparing B2 is 50°C to 60°C, the feed speed is 850rpm to 1200rpm, and the classification speed is 620rpm to 680rpm; In step (2), the temperature for preparing G1 is maintained at 25°C-30°C; the material temperature for preparing G2 is 50°C-60°C, the feed speed is 800rpm-1000rpm, and the classification speed is 600rpm-650rpm; In step (3), the process comprises first mixing the B and G in proportion to obtain a mixture BG, and then mixing the mixture with a filler matrix in proportion to obtain the microbial fertilizer.

8. A method for preparing the microbial fertilizer according to any one of claims 1 to 7, characterized in that: The preparation method comprises the following steps: The microbial fertilizer is obtained by mixing the precipitate of the fermentation liquid of Streptomyces lilacinus Hainan variant, the inclusion agent, the adsorbent, the filler matrix, the carbon source, the nitrogen source and the inorganic salt.

9. The preparation method according to claim 8, characterized in that: The preparation method comprises the following steps: (1) mixing the precipitate with the inclusion agent in proportion to obtain B1; mixing the carbon source, nitrogen source and inorganic salt with the inclusion agent in proportion to obtain B2; (2) mixing the B1 and the adsorbent in proportion to obtain the G1; mixing the B2 and the adsorbent in proportion to obtain the G2; (3) mixing the B1, B2, G1, G2 and / or the filler matrix in proportion to obtain the microbial fertilizer; Wherein, in step (1), the temperature for preparing B1 is 25°C-30°C, and stirring is continued for 60 minutes; the material temperature for preparing B2 is 50°C-60°C, the feed speed is 850rpm-1200rpm, and the classification speed is 620rpm-680rpm; In step (2), the temperature for preparing G1 is 25°C-30°C; the material temperature for preparing G2 is 50°C-60°C, the feed speed is 800rpm-1000rpm, and the classification speed is 600rpm-650rpm; In step (3), the B and G are first mixed in proportion to obtain a mixture BG, and then mixed with a filler matrix in proportion to obtain the microbial fertilizer.

10. Use of the microbial fertilizer according to any one of claims 1 to 7 in increasing crop yield.

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