Device for collecting saliva extract of leech

By designing a leech saliva extraction device including anti-reverse tube, negative pressure part and shaking part, the problem of unsatisfactory leech saliva secretion effect and easy leech damage in the prior art is solved, and more efficient saliva extraction and leech protection are achieved.

CN119798361AActive Publication Date: 2025-04-11SHANDONG YOUREN TRADITIONAL CHINESE MEDICINE CO LTD

Patent Information

Application Number
CN202510170642.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-17
Publication Date
2025-04-11
Estimated Expiration
2045-02-17

AI Technical Summary

Technical Problem

The existing leech saliva extraction device squeezes the leech through two funnels to secrete saliva, but the saliva secretion effect is not ideal, and oversqueezing will cause the leech to die.

Method used

A device is designed, including an outer frame, a saliva extraction part, a separation part, a negative pressure part and a jitter part. By extruding leeches in the inner wall of the anti-reverse tube, combining negative pressure and jitter mechanisms, a more uniform leeches' force and effective extraction of saliva is achieved.

Benefits of technology

Through embracing squeezing and negative pressure suction, the device improves the secretion effect of leech saliva, reduces the damage and mortality of leech, and effectively separates leech from its saliva.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of leech saliva extraction, in particular to a device for collecting leech saliva extracts.The device comprises an outer frame, a saliva extraction part, a separation part, a negative pressure part and a shaking part, four negative pressure openings distributed at equal angles are formed in the outer wall of the outer frame in a penetrating mode, and the saliva extraction part is fixedly installed at the top of the outer frame and extends into the outer frame. The body of the leech is extruded by the inner wall of the anti-reverse pipe, the leech continuously spits saliva after being pressed, and the conical extrusion cylinder and the anti-reverse pipe are circular, so that the extrusion of the conical extrusion cylinder and the inner wall of the anti-reverse pipe on the body of the leech is in a surrounding mode in the downward moving process of water drops, the stress on the body of the leech is more uniform, and the saliva effect of the leech is more ideal; and compared with the existing means, the condition that the leeches are easily crushed due to local extrusion is also avoided, and the inclined downward anti-reversing ring groove is formed in the inner wall of the anti-reversing pipe, so that the leeches cannot creep back after entering the anti-reversing pipe.
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Description

Technical Field

[0001] The invention relates to the technical field of leech saliva extraction, in particular to a device for collecting leech saliva extract. Background Art

[0002] Hirudin is an anticoagulant protein extracted from medical leeches. Studies have found that a large amount of hirudin can be extracted from leeches and their salivary gland secretions. A Chinese patent with reference announcement number CN118406137B discloses a device and method for collecting leech saliva extracts. The active leeches are squeezed twice by the movable plate and the squeezing plate in the second funnel, thereby increasing the yield of hirudin. Leeches can be screened out by their own activity. This method requires less experience from the staff and helps to reduce costs. The leech can be collected again to increase the amount of hirudin collected, and the electrodes will not cause excessive stimulation to the leeches, so that the survival rate of the leeches can be improved, and the death of the leeches can be avoided. However, the device for extracting leech saliva squeezes the leeches through the upper and lower funnels to make them secrete saliva, which can only make the upper and lower parts of the leech body bear force, and the saliva secretion effect is not ideal. In order to better obtain the leech saliva, if the squeezing force is too large, the leeches will be over-pressed and die. For this reason, we propose a device for collecting leech saliva extract to solve the above technical problems. Summary of the invention

[0003] The present invention provides the following technical solution: a device for collecting leech saliva extract, an outer frame, wherein the outer wall of the outer frame is penetrated by four negative pressure ports distributed at equal angles; The saliva extraction part is fixedly mounted on the top of the outer frame and extends to the inside of the outer frame for collecting leech saliva; A separation part, movably mounted inside the outer frame and extending to the bottom thereof, for collecting leech saliva and leeches; The negative pressure part is fixedly mounted on the outer wall of the outer frame in a circumferential array form and is used to generate negative pressure inside the outer frame; The shaking part is fixedly mounted on the inner wall of the outer frame in the form of a circular array and is used for the oscillation of the separation part.

[0004] As a preferred embodiment of the present invention, the saliva extraction unit comprises: A baffle, fixedly mounted on the top of the outer frame; A plurality of guide holes are provided through the top of the baffle plate; The bucket is fixedly mounted on the top of the baffle plate and is located outside the plurality of guide holes; The conical extrusion cylinder is fixedly mounted on the bottom of the baffle and is located outside the opening of the guide hole, and the diameter of the bucket gradually decreases downward; The anti-reverse pipe is fixedly installed at the bottom of the conical extrusion cylinder and is connected with the inside of the conical extrusion cylinder.

[0005] As a preferred solution of the present invention, the separation unit includes: The saliva drainage tube is located at the bottom of the anti-retrograde tube; A saliva drainage component is fixedly installed on the top of the saliva drainage tube, and the saliva drainage component is arranged in a tower shape; It receives the cone bucket and is slidably mounted on the inner wall of the outer frame; The assembly holes are formed through the top of the receiving cone bucket, and the number and position of the holes correspond to the saliva drainage tubes one by one. The saliva drainage tubes are fixedly installed inside the assembly holes at the corresponding positions. Collect roar and fix it at the bottom of the assembly hole; The collecting bottle is screwed to the lower part of the outer wall of the collecting bottle through threads; The saliva receiving groove is screwed to the bottom of the outer frame through threads, and the inner wall of the saliva receiving groove is slidably connected with the outer wall of the collecting jar.

[0006] As a preferred solution of the present invention, the negative pressure unit includes: The negative pressure pump housing is fixedly mounted on the outer wall of the outer frame and is located outside the opening of the negative pressure port; The diaphragm is fixedly mounted on the middle of the inner wall of the negative pressure pump housing; The electric cylinder is fixedly mounted on a side of the negative pressure pump housing away from the outer frame, and the output rod of the electric cylinder movably extends to the inside of the negative pressure pump housing and is fixedly connected to the middle of the diaphragm; The flow guide cover is fixedly mounted on a side of the negative pressure pump housing away from the electric cylinder and is communicated with the interior of the negative pressure pump housing. The flow guide cover extends to the interior of the negative pressure port.

[0007] As a preferred solution of the present invention, the negative pressure unit further includes: A one-way valve is fixedly mounted on the bottom of the negative pressure pump housing at one end away from the electric cylinder, and the one-way valve is connected to the interior of the negative pressure pump housing; The conical spring is sleeved on the outer periphery of the output rod of the electric cylinder and fixedly installed between a side of the diaphragm away from the outer frame and an inner wall of one side of the negative pressure pump housing; The air holes are horizontally opened at one end of the outer wall of the negative pressure pump housing close to the electric cylinder, and there are two of them. The two air holes are symmetrically distributed about the center of the negative pressure pump housing.

[0008] As a preferred solution of the present invention, the shaking unit includes: The cylinder body is fixedly mounted on the inner wall of the outer frame in the form of a circular array and is located between two adjacent negative pressure ports; A piston is slidably mounted on the inner wall of the cylinder; A push rod is fixedly mounted on the bottom of the piston, and the bottom of the push rod is fixedly connected to the top edge of the receiving cone bucket; An air supply main pipe is fixedly mounted on the top of the cylinder body and communicated with the interior of the cylinder body, and the air supply main pipe penetrates the side wall of the outer frame and extends to the periphery thereof; The air supply manifold is fixedly installed at one end of the air supply main pipe away from the cylinder body and is communicated with the interior of the air supply main pipe. The two ends of the air supply manifold are fixedly connected to the ends of two adjacent air holes.

[0009] As a preferred solution of the present invention, the diameter of the saliva drainage component is gradually increased downward, and the bottom diameter of the saliva drainage component is larger than the inner diameter of the anti-reverse tube. The saliva drainage component includes a plurality of pleated saliva drainage guide strips in a circular array formed on the top of the saliva drainage tube.

[0010] As a preferred solution of the present invention, the saliva receiving trough is open at the top and is located at the bottom of multiple saliva drainage tubes. A shut-off valve is fixedly installed on the bottom output end of the saliva receiving trough, and a discharge pipe is fixedly installed on the output end of the shut-off valve.

[0011] As a preferred solution of the present invention, a plurality of anti-reverse ring grooves equidistantly distributed vertically are provided on the inner wall of the anti-reverse tube, and the cross-section of the anti-reverse ring grooves is arranged obliquely downward.

[0012] As a preferred solution of the present invention, four legs distributed at equal angles are fixedly arranged on the outer wall of the outer frame.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. In the present invention, the body of the leech is squeezed by the inner wall of the anti-reverse tube, and the leech continues to spit out saliva after being squeezed. Since the conical squeezing tube and the anti-reverse tube are circular, the conical squeezing tube and the inner wall of the anti-reverse tube squeeze the leech body in an embracing manner during the downward movement of the water droplets, so that the leech body is more evenly stressed and the leech saliva effect is more ideal. Compared with the existing means, it also avoids the situation that the leech is easily crushed due to local squeezing. In addition, the inner wall of the anti-reverse tube is provided with an anti-reverse ring groove obliquely downward, so that the leech cannot crawl back after entering the anti-reverse tube.

[0014] 2. In the present invention, the middle part of the diaphragm is driven to move away from the outer frame by the output shaft of the electric cylinder, causing the compression conical spring to store force, and at the same time causing a negative pressure effect inside the negative pressure pump housing and inside the outer frame. The one-way valve is closed, and due to the negative pressure inside the outer frame, a downward suction effect can be generated on the leeches inside the anti-reverse tube, thereby accelerating the speed at which the leeches move downward along the inner wall of the anti-reverse tube, while also preventing the leeches from getting stuck inside the anti-reverse tube.

[0015] 3. In the present invention, when the middle part of the diaphragm moves to the maximum position in the direction away from the outer frame, the electric cylinder is powered off, and the rebound force of the conical spring is released, pushing the diaphragm to reset. At this time, the one-way valve is opened for ventilation, and external air enters the interior of the negative pressure pump housing through the one-way valve, and replenishes air to the interior of the outer frame through the guide cover, thereby preventing leeches from suffocating to death.

[0016] 4. In the present invention, during the continuous cyclic movement of the diaphragm, the receiving cone bucket shakes up and down, and some leeches stuck between the top of the receiving cone bucket and the outer wall of the saliva drainage tube are shaken into the interior of the collecting bottle, and then collected in the interior of the collecting bottle, thereby preventing the leeches from being stuck between the top of the receiving cone bucket and the outer wall of the saliva drainage tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of the present invention; Figure 2 For the present invention Figure 1 A schematic diagram of a partial cross-sectional structure; Figure 3 For the present invention Figure 2 A schematic diagram of the enlarged structure of part A; Figure 4 It is a schematic diagram of the internal structure of the outer frame in the present invention; Figure 5 It is a structural schematic diagram of the negative pressure part in the present invention; Figure 6 It is a schematic diagram of the top cross-sectional structure of the negative pressure pump casing of the present invention; Figure 7 It is a schematic diagram of the structure of the saliva drainage component in the present invention; Figure 8 For the present invention Figure 7 A schematic diagram of the enlarged structure of part B; Fig. 9 It is a structural schematic diagram of the receiving cone bucket in the present invention; Fig.10 It is a schematic diagram of the side section structure of the cylinder body in the present invention; Fig.11 It is a schematic diagram of the side section structure of the anti-reverse tube in the present invention.

[0018] In the figure: 100, outer frame; 101, negative pressure port; 200, saliva extraction part; 201, baffle; 202, guide hole; 203, bucket; 204, conical extrusion cylinder; 205, anti-reverse pipe; 2051, anti-reverse ring groove; 300, separation part; 301, saliva drainage tube; 302, saliva drainage assembly; 3021, saliva drainage guide strip; 303, receiving cone bucket; 304, assembly hole; 305, collection roar; 306 , collecting bottle; 307, saliva receiving tank; 308, shut-off valve; 309, discharge pipe; 400, negative pressure part; 401, negative pressure pump housing; 402, diaphragm; 403, electric cylinder; 404, guide cover; 405, one-way valve; 406, conical spring; 407, air hole; 500, shaking part; 501, cylinder body; 502, piston; 503, push rod; 504, air supply main pipe; 505, air supply manifold; 600, support leg. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figures 1 to 11 The technical solution provided by the present invention specifically includes the following embodiments: A device for collecting leech saliva extract comprises an outer frame 100, a saliva extraction part 200, a separation part 300, a negative pressure part 400 and a shaking part 500. The outer wall of the outer frame 100 is penetrated with four negative pressure ports 101 distributed at equal angles. The saliva extraction part 200 is fixedly mounted on the top of the outer frame 100 and extends to the inside of the outer frame 100 for collecting leech saliva. The separation part 300 is movably mounted on the inside of the outer frame 100 and extends to the bottom thereof for collecting leech saliva and leeches. The negative pressure part 400 is fixedly mounted on the outer wall of the outer frame 100 in a circular array for generating negative pressure inside the outer frame 100. The shaking part 500 is fixedly mounted on the inner wall of the outer frame 100 in a circular array for oscillating the separation part 300. Four legs 600 distributed at equal angles are fixedly arranged on the outer wall of the outer frame 100 for supporting and fixing the device for extracting leech saliva extract as a whole.

[0021] For further details, please refer to Figure 2 , Figure 3 as well as Fig.11 As shown: The saliva extraction part 200 includes a baffle 201, a guide hole 202, a bucket 203, a conical extrusion cylinder 204 and an anti-reverse tube 205. The baffle 201 is fixedly installed on the top of the outer frame 100. The guide holes 202 are opened through the top of the baffle 201, and there are multiple of them. The bucket 203 is fixedly installed on the top of the baffle 201 and is located outside the multiple guide holes 202. The conical extrusion cylinder 204 is fixedly installed on the bottom of the baffle 201 and is located outside the opening of the guide holes 202. The diameter of the bucket 203 gradually decreases downward. The anti-reverse tube 205 is fixedly installed on the bottom of the conical extrusion cylinder 204 and is connected to the inside of the conical extrusion cylinder 204. The inner wall of the anti-reverse tube 205 is provided with multiple anti-reverse ring grooves 2051 distributed equidistantly up and down, and the cross-section of the anti-reverse ring groove 2051 is set obliquely downward.

[0022] Specifically, the leech is put into the bucket 203. Under the action of gravity, the leech creeps into the conical extrusion tube 204 through the guide hole 202. Since the top diameter of the conical extrusion tube 204 is larger than the bottom diameter thereof, it is convenient for the leech to creep into the conical extrusion tube 204. As the leech creeps downward in the conical extrusion tube 204, the inner wall of the conical extrusion tube 204 squeezes the leech body and gradually shrinks. In this process, the leech saliva can be squeezed out. The squeezed leech saliva flows downward along the inner wall of the conical extrusion tube 204 and the anti-reversal tube 205. At the same time, the leech continues to creep downward into the anti-reversal tube 205. The anti-reverse ring groove 2051 inclined downward is provided on the wall to limit the body of the leech. That is to say, after the leech enters the anti-reverse tube 205, it cannot crawl back and can only continue to crawl downward. During this period, the body of the leech continues to be squeezed by the inner wall of the anti-reverse tube 205. The leech continues to spit out saliva after being pressed. Since the conical extrusion cylinder 204 and the anti-reverse tube 205 are circular, the conical extrusion cylinder 204 and the inner wall of the anti-reverse tube 205 squeeze the leech body in an embracing manner during the downward movement of the water droplets, so that the leech body is more evenly stressed and the leech saliva effect is more ideal. Compared with the existing means, it also avoids the situation where the leech is easily crushed due to local squeezing.

[0023] For further details, please refer to Figure 4 , Figure 5 , Figure 7 , Figure 8 , Fig. 9 As shown: The separation part 300 includes a saliva drainage tube 301, a saliva drainage component 302, a receiving cone 303, an assembly hole 304, a collecting roar 305, a collecting bottle 306 and a saliva receiving groove 307. The saliva drainage tube 301 is located at the bottom of the anti-reverse tube 205, and the saliva drainage component 302 is fixedly installed on the top of the saliva drainage tube 301. The saliva drainage component 302 is arranged in a tower shape, and the diameter of the saliva drainage component 302 gradually increases downward, and the bottom diameter of the saliva drainage component 302 is larger than the inner diameter of the anti-reverse tube 205. The saliva drainage component 302 includes a plurality of pleated saliva drainage guide strips 3021 in a circumferential array on the top of the saliva drainage tube 301. The receiving cone 303 is slidably installed on the inner wall of the outer frame 100. The assembly hole 30 4 is provided through the top of the receiving cone bucket 303, and the number and position correspond to the saliva drainage tubes 301 one by one. The saliva drainage tubes 301 are fixedly installed inside the assembly holes 304 at the corresponding positions. The collecting bell 305 is fixedly installed at the bottom of the assembly holes 304. The collecting bottle 306 is screwed to the lower part of the outer wall of the collecting bell 305 through threads. The saliva receiving groove 307 is screwed to the bottom of the outer frame 100 through threads, and the inner wall of the saliva receiving groove 307 is slidably connected to the outer wall of the collecting bell 305. The saliva receiving groove 307 is open at the top and is located at the bottom of the plurality of saliva drainage tubes 301. A shut-off valve 308 is fixedly installed on the bottom output end of the saliva receiving groove 307, and a discharge pipe 309 is fixedly installed on the output end of the shut-off valve 308.

[0024] Specifically, the leech spits out saliva after being squeezed by the inner wall of the anti-reverse tube 205, and the spits out saliva drips along the inner wall of the anti-reverse tube 205 to the surface of the saliva drainage strip 3021. Since the saliva drainage strip 3021 is arranged in a folded shape, the leech saliva fluid attached to the surface of the saliva drainage strip 3021 flows downward along the surface of the saliva drainage strip 3021 to the folds and then drips into the inside of the saliva drainage tube 301, and does not flow to the outer wall of the saliva drainage tube 301. , and then discharged into the saliva receiving groove 307 through the inner wall of the saliva drainage tube 301 for collection. The leech that has spit out saliva finally crawls out from the bottom of the anti-reverse tube 205, and falls on the surface of the saliva drainage component 302 under the action of gravity, and slides along the surface of the saliva drainage component 302 to the inside of the receiving cone bucket 303, and is discharged into the collection bottle 306 through the collection roar 305 for collection. In this way, after the saliva of the leech is extracted, the leech can be separated from its saliva.

[0025] For further details, please refer to Figures 4 to 6 As shown: The negative pressure part 400 includes a negative pressure pump housing 401, a diaphragm 402, an electric cylinder 403, a flow guide 404, a one-way valve 405, a conical spring 406 and an air hole 407. The negative pressure pump housing 401 is fixedly mounted on the outer wall of the outer frame 100 and is located outside the opening of the negative pressure port 101. The diaphragm 402 is fixedly mounted on the middle of the inner wall of the negative pressure pump housing 401. The electric cylinder 403 is fixedly mounted on a side of the negative pressure pump housing 401 away from the outer frame 100. The output rod of the electric cylinder 403 movably extends to the interior of the negative pressure pump housing 401 and is fixedly connected to the middle of the diaphragm 402. The flow guide 404 is fixedly mounted on a side of the negative pressure pump housing 401 away from the electric cylinder 403. The one-way valve 405 is fixedly mounted on the bottom of the negative pressure pump housing 401 at one end away from the electric cylinder 403, and is communicated with the interior of the negative pressure pump housing 401. The one-way valve 405 is communicated with the interior of the negative pressure pump housing 401. The conical spring 406 is sleeved on the periphery of the output rod of the electric cylinder 403, and is fixedly mounted between the side of the diaphragm 402 away from the outer frame 100 and the inner wall of one side of the negative pressure pump housing 401. The air hole 407 is horizontally opened on the outer wall of the negative pressure pump housing 401 close to the end of the electric cylinder 403, and there are two air holes 407, which are symmetrically distributed about the center of the negative pressure pump housing 401.

[0026] Specifically, by starting the electric cylinder 403, its output shaft drives the middle part of the diaphragm 402 to move away from the outer frame 100, causing the compression conical spring 406 to accumulate force, and at the same time causing a negative pressure effect to be generated inside the negative pressure pump housing 401 and the inner part of the outer frame 100. The one-way valve 405 is closed. Due to the negative pressure inside the outer frame 100, a downward suction effect can be generated on the leeches located inside the anti-reverse tube 205, thereby accelerating the speed at which the leeches move downward along the inner wall of the anti-reverse tube 205, and also preventing the leeches from being stuck inside the anti-reverse tube 205. When the middle part of the diaphragm 402 moves to the maximum position in the direction away from the outer frame 100, the electric cylinder 403 is powered off, and the rebound force of the conical spring 406 is released, pushing the diaphragm 402 to reset. At this time, the one-way valve 405 is opened for ventilation, and external air enters the interior of the negative pressure pump housing 401 through the one-way valve 405, and replenishes air to the interior of the outer frame 100 through the air guide cover 404, thereby preventing the leeches from suffocating to death.

[0027] For further details, please refer to Figure 4 , Figure 5 , Figure 6 as well as Fig.10 As shown: The shaking part 500 includes a cylinder body 501, a piston 502, a push rod 503, an air supply main pipe 504 and an air supply manifold 505. The cylinder body 501 is fixedly mounted on the inner wall of the outer frame 100 in the form of a circular array and is located between two adjacent negative pressure ports 101. The piston 502 is slidably mounted on the inner wall of the cylinder body 501. The push rod 503 is fixedly mounted on the bottom of the piston 502. The bottom of the push rod 503 is fixedly connected to the top edge of the receiving cone bucket 303. The air supply main pipe 504 is fixedly mounted on the top of the cylinder body 501 and is connected to the interior of the cylinder body 501. The air supply main pipe 504 passes through the side wall of the outer frame 100 and extends to its periphery. The air supply manifold 505 is fixedly mounted on one end of the air supply main pipe 504 away from the cylinder body 501 and is connected to the interior of the air supply main pipe 504. Both ends of the air supply manifold 505 are fixedly connected to the ends of two adjacent air holes 407.

[0028] Specifically, when the middle of the diaphragm 402 moves away from the outer frame 100, the area inside the negative pressure pump housing 401 near one end of the electric cylinder 403 is compressed, and the air in this area is pressed into the interior of the cylinder body 501 through the air hole 407, the air supply manifold 505 and the air supply main pipe 504, thereby pushing the piston 502 and the push rod 503 downward. The push rod 503 moves downward and drives the receiving cone bucket 303 to move downward together. As the middle of the diaphragm 402 is reset, the compressed area inside the negative pressure pump housing 401 near one end of the electric cylinder 403 is restored, and the air injected into the cylinder The air inside the body 501 is drawn back into the interior of the negative pressure pump housing 401 through the air supply main pipe 504, the air supply manifold 505 and the air hole 407, causing the piston 502 to drive the push rod 503 and the receiving cone bucket 303 to move upward. That is to say, during the continuous circulation movement of the diaphragm 402, the receiving cone bucket 303 shakes up and down, and some leeches stuck between the top of the receiving cone bucket 303 and the outer wall of the saliva drainage tube 301 are shaken into the interior of the collecting bottle 305, and then collected in the interior of the collecting bottle 306, so as to prevent the leeches from being stuck between the top of the receiving cone bucket 303 and the outer wall of the saliva drainage tube 301.

[0029] The device for collecting leech saliva extract in this scheme is divided into the following processes when working: Leech saliva extraction: leeches are placed into the bucket 203. Under the action of gravity, the leeches creep through the guide hole 202 to the inside of the conical extrusion cylinder 204. Since the top diameter of the conical extrusion cylinder 204 is larger than the bottom diameter, it is convenient for the leeches to creep into the conical extrusion cylinder 204. As the leeches creep downward in the conical extrusion cylinder 204, the inner wall of the conical extrusion cylinder 204 squeezes the leech body and gradually shrinks. In this process, the leeches can be extracted. The leech saliva is squeezed out, and the squeezed leech saliva flows downward along the inner wall of the conical extrusion cylinder 204 and the anti-reverse tube 205. At the same time, the leech continues to crawl downward into the anti-reverse tube 205. Since the anti-reverse ring groove 2051 is arranged on the inner wall of the anti-reverse tube 205, the body of the leech can be limited. That is to say, after the leech enters the anti-reverse tube 205, it cannot crawl back and can only continue to crawl downward. During this period, the body of the leech continues to crawl downward. The leech is squeezed by the inner wall of the anti-reverse tube 205, and continues to spit out saliva after being pressed. During this period, the saliva spit out by the leech drips onto the surface of the saliva drainage strip 3021. Since the saliva drainage strip 3021 is arranged in a folded shape, the leech saliva fluid attached to the surface of the saliva drainage strip 3021 flows downward along the surface of the saliva drainage strip 3021 to the folds and then drips into the inside of the saliva drainage tube 301, and does not flow to the outer wall of the saliva drainage tube 301. The saliva is discharged into the saliva receiving groove 307 through the inner wall of the saliva drainage tube 301 for collection, and the leech that has spit out saliva finally crawls out from the bottom of the anti-reverse tube 205, and falls on the surface of the saliva drainage component 302 under the action of gravity, and slides along the surface of the saliva drainage component 302 to the inside of the receiving cone bucket 303, and is discharged into the inside of the collection bottle 306 through the collection bottle 305 for collection. In this way, after the saliva of the leech is extracted, the leech can be separated from its saliva; Assisting leech peristalsis: By starting the electric cylinder 403, its output shaft drives the middle of the diaphragm 402 to move away from the outer frame 100, causing the compression conical spring 406 to store force, and at the same time, a negative pressure effect is generated inside the negative pressure pump housing 401 and the inner part of the outer frame 100. The one-way valve 405 is closed. Due to the negative pressure inside the outer frame 100, a downward suction effect can be generated on the leech inside the anti-reverse tube 205, accelerating the leech to move downward along the inner wall of the anti-reverse tube 205. The speed of movement is increased, and the leech is prevented from getting stuck in the anti-reverse tube 205. When the middle of the diaphragm 402 moves to the maximum position in the direction away from the outer frame 100, the electric cylinder 403 is powered off, and the rebound force of the conical spring 406 is released, pushing the diaphragm 402 to reset. At this time, the one-way valve 405 is opened for ventilation, and the external air enters the interior of the negative pressure pump housing 401 through the one-way valve 405, and replenishes the air to the interior of the outer frame 100 through the guide cover 404, thereby preventing the leech from suffocating to death. Assisted leech collection: When the middle of the diaphragm 402 moves away from the outer frame 100, the area inside the negative pressure pump housing 401 near one end of the electric cylinder 403 is compressed, and the air in this area is pressed into the cylinder body 501 through the air hole 407, the air supply manifold 505 and the air supply main pipe 504, thereby pushing the piston 502 and the push rod 503 downward. The push rod 503 moves downward and drives the receiving cone bucket 303 to move downward together. As the middle of the diaphragm 402 is reset, the compressed area inside the negative pressure pump housing 401 near one end of the electric cylinder 403 is restored, and the injected The air inside the cylinder 501 is drawn back into the interior of the negative pressure pump housing 401 through the air supply main pipe 504, the air supply manifold 505 and the air hole 407, causing the piston 502 to drive the push rod 503 and the receiving cone bucket 303 to move upward. That is to say, during the continuous circulation movement of the diaphragm 402, the receiving cone bucket 303 shakes up and down, and some leeches stuck between the top of the receiving cone bucket 303 and the outer wall of the saliva drainage tube 301 are shaken into the interior of the collecting bottle 305, and then collected in the interior of the collecting bottle 306, so as to prevent the leeches from being stuck between the top of the receiving cone bucket 303 and the outer wall of the saliva drainage tube 301.

[0030] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A device for collecting leech saliva extract, characterized in that: include: An outer frame (100), wherein the outer wall of the outer frame (100) is penetrated by four negative pressure ports (101) distributed at equal angles; A saliva extraction portion (200) is fixedly mounted on the top of the outer frame (100) and extends into the interior of the outer frame (100) and is used for collecting leech saliva; A separation portion (300) is movably mounted inside the outer frame (100) and extends to the bottom thereof, and is used for collecting leech saliva and leeches; The negative pressure portion (400) is fixedly mounted on the outer wall of the outer frame (100) in a circumferential array form and is used to generate negative pressure inside the outer frame (100); The shaking part (500) is fixedly mounted on the inner wall of the outer frame (100) in the form of a circular array and is used for shaking the separation part (300).

2. A device for collecting leech saliva extract according to claim 1, characterized in that: The saliva extraction unit (200) comprises: A baffle (201) is fixedly mounted on the top of the outer frame (100); A plurality of guide holes (202) are formed through the top of the baffle (201); A bucket (203) is fixedly mounted on the top of the baffle (201) and is located outside the plurality of guide holes (202); The conical extrusion cylinder (204) is fixedly mounted on the bottom of the baffle (201) and is located outside the opening of the guide hole (202), and the diameter of the bucket (203) gradually decreases downward; The anti-reversal tube (205) is fixedly mounted on the bottom of the conical extrusion cylinder (204) and is communicated with the interior of the conical extrusion cylinder (204).

3. A device for collecting leech saliva extract according to claim 2, characterized in that: The separation unit (300) comprises: A saliva drainage tube (301) located at the bottom of the anti-reversal tube (205); A saliva drainage component (302) is fixedly mounted on the top of the saliva drainage tube (301), wherein the saliva drainage component (302) is arranged in a tower shape; A receiving cone bucket (303) is slidably mounted on the inner wall of the outer frame (100); The assembly holes (304) are formed through the top of the receiving cone (303), and the number and position of the holes correspond to the saliva drainage tubes (301) one by one. The saliva drainage tubes (301) are fixedly installed inside the assembly holes (304) at the corresponding positions; A collecting roar (305) is fixedly mounted at the bottom of the assembly hole (304); The collecting bottle (306) is screwed to the lower part of the outer wall of the collecting bottle (305) through a thread; The saliva receiving groove (307) is screwed to the bottom of the outer frame (100) via a thread, and the inner wall of the saliva receiving groove (307) is slidably connected to the outer wall of the collecting chamber (305).

4. A device for collecting leech saliva extract according to claim 3, characterized in that: The negative pressure unit (400) comprises: A negative pressure pump housing (401) is fixedly mounted on the outer wall of the outer frame (100) and is located outside the opening of the negative pressure port (101); The diaphragm (402) is fixedly mounted on the middle part of the inner wall of the negative pressure pump housing (401); The electric cylinder (403) is fixedly mounted on a side of the negative pressure pump housing (401) away from the outer frame (100), and the output rod of the electric cylinder (403) movably extends into the interior of the negative pressure pump housing (401) and is fixedly connected to the middle of the diaphragm (402); The flow guide cover (404) is fixedly mounted on a side of the negative pressure pump housing (401) away from the electric cylinder (403) and is communicated with the interior of the negative pressure pump housing (401). The flow guide cover (404) extends to the interior of the negative pressure port (101).

5. The device for collecting leech saliva extract according to claim 4, characterized in that: The negative pressure unit (400) further includes: A one-way valve (405) is fixedly mounted on the bottom of the negative pressure pump housing (401) at one end away from the electric cylinder (403), and the one-way valve (405) is connected to the interior of the negative pressure pump housing (401); The conical spring (406) is sleeved around the outer periphery of the output rod of the electric cylinder (403) and is fixedly installed between a side surface of the diaphragm (402) away from the outer frame (100) and an inner wall of one side of the negative pressure pump housing (401); The air holes (407) are horizontally opened on one end of the outer wall of the negative pressure pump housing (401) close to the electric cylinder (403), and there are two of them. The two air holes (407) are symmetrically distributed about the center of the negative pressure pump housing (401).

6. The device for collecting leech saliva extract according to claim 5, characterized in that: The shaking unit (500) comprises: The cylinder body (501) is fixedly mounted on the inner wall of the outer frame (100) in the form of a circular array and is located between two adjacent negative pressure ports (101); A piston (502) is slidably mounted on the inner wall of the cylinder (501); A push rod (503) is fixedly mounted on the bottom of the piston (502), and the bottom of the push rod (503) is fixedly connected to the top edge of the receiving cone bucket (303); An air supply main pipe (504) is fixedly mounted on the top of the cylinder body (501) and is in communication with the interior of the cylinder body (501); the air supply main pipe (504) penetrates the side wall of the outer frame (100) and extends to the periphery thereof; The air supply manifold (505) is fixedly mounted on one end of the air supply main pipe (504) away from the cylinder body (501) and is communicated with the interior of the air supply main pipe (504). The two ends of the air supply manifold (505) are fixedly connected to the ends of two adjacent air holes (407).

7. A device for collecting leech saliva extract according to claim 6, characterized in that: The diameter of the saliva drainage component (302) gradually increases downwards, and the bottom diameter of the saliva drainage component (302) is larger than the inner diameter of the anti-reversal tube (205). The saliva drainage component (302) comprises a plurality of pleated saliva drainage guide strips (3021) arranged in a circular array on the top of the saliva drainage tube (301).

8. The device for collecting leech saliva extract according to claim 7, characterized in that: The saliva receiving groove (307) is open at the top and is located at the bottom of the plurality of saliva drainage tubes (301). A shut-off valve (308) is fixedly mounted on the bottom output end of the saliva receiving groove (307), and a discharge pipe (309) is fixedly mounted on the output end of the shut-off valve (308).

9. The device for collecting leech saliva extract according to claim 8, characterized in that: The inner wall of the anti-reverse pipe (205) is provided with a plurality of anti-reverse ring grooves (2051) distributed at equal distances up and down, and the cross-section of the anti-reverse ring grooves (2051) is arranged obliquely downward.

10. The device for collecting leech saliva extract according to claim 9, characterized in that: Four supporting legs (600) distributed at equal angles are fixedly arranged on the outer wall of the outer frame (100).

Citation Information

Patent Citations

  • Device and method for extracting hirudin from live leeches in laboratory

    CN110734489A

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    CN118406137A

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