Double-sampling rapid detection device for zoonosis pathogen and use method of double-sampling rapid detection device

Abstract

The invention discloses a double-sampling zoonosis pathogen rapid detection device and a use method, the double-sampling zoonosis pathogen rapid detection device comprises a combined body, incubators, a collector and a detection tester, the incubators are fixedly installed in the two ends of the combined body, the detection tester is fixedly installed in the middle of the combined body, and the collector is fixedly installed in the middle of the combined body. Collectors are embedded in the upper surfaces of the two ends of the combined body; body fluid sampling, including but not limited to blood or saliva, is carried out on livestock and human bodies through a sampling needle in a collector, after timed culture is carried out through a culture solution in an incubator, the culture solution containing the sampled body fluid is guided into a detection tester, and corresponding pathogen antigen detection is carried out through detection test paper fixedly arranged in the detection tester; in the whole detection process, the leakproofness of the process is realized, the influence of external environment pathogens is reduced, the detection time is greatly shortened, and the detection convenience is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of detection devices, and in particular to a dual-sampling zoonotic pathogen rapid detection device and a use method thereof. Background Art

[0002] In the process of animal husbandry, livestock can be infected with various diseases, some of which can be transmitted to humans. Once a large area of ​​zoonotic infection occurs, it is very easy to cause huge economic losses and affect the development of animal husbandry. However, the symptoms of the same pathogen infection in livestock and humans are different, so the detection process is relatively complicated. The existing detection methods generally require sampling from both livestock and humans, and then processing them in various ways. The process is complicated and time-consuming. When testing on-site, pathogens may also exist in the air, which makes the detection process prone to contamination.

[0003] Therefore, it is necessary to design a dual-sampling rapid detection device and method for zoonotic pathogens. Summary of the invention

[0004] The purpose of the present invention is to provide a dual-sampling zoonotic pathogen rapid detection device and a method of use to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A dual-sampling rapid detection device for zoonotic pathogens comprises a combined body, an incubator, a collector and a detection tester. The incubator is fixedly installed in both ends of the combined body, the detection tester is fixedly installed in the middle of the combined body, and the collectors are embedded on the upper surfaces of both ends of the combined body.

[0007] According to the above technical scheme, the combined union includes a connecting bridge, a first end head and a second end head, the first end head and the second end head are respectively integrally formed at the two ends of the connecting bridge, the first end head and the second end head are centrally symmetrical structures, the upper surface of the first end head is integrally formed with a placement groove, a collector is embedded in the placement groove, the upper shell of the first end head is provided with an exposed port, the exposed port is located at the head end of the placement groove, the wall surface of the rear end of the placement groove is provided with a push port, the incubator is fixedly installed in conjunction with the exposed port and the push port, a guide tube is fixedly installed in the first end head, one end of the guide tube is matched with the liquid outlet of the incubator, and the other end of the guide tube cooperates with the dripping end of the detection tester.

[0008] According to the above technical solution, the culture vessel includes a liquid storage tube, an injection tube and a liquid outlet tube. A piston is slidably installed in the liquid storage tube and is filled with culture fluid. The injection tube is integrally formed on the upper surface of the liquid storage tube. The injection tube is plugged with a rubber sealing plug, and the rubber sealing plug cooperates with the exposed port. The liquid outlet tube is integrally formed on the lower surface of the liquid storage tube. A one-way valve is fixedly installed in the liquid outlet tube, and the liquid outlet tube and one end of the guide tube are mutually matched.

[0009] According to the above technical solution, the collector includes a holding body, a combination cap and a collecting needle. A temporary positioning groove is provided in the holding body, and the collecting needle is clamped in the temporary positioning groove. The combination cap is inserted into the head of the holding body. The combination cap is provided with a receiving groove, and the head of the collecting needle is located in the receiving groove. A limiting ring is integrally formed on the head of the collecting needle, and a breaking mark is provided at the tail of the collecting needle.

[0010] According to the above technical solution, the detection tester includes a shell, a titration port is provided on the upper surface of the head of the shell, a detection test paper is fixed in the shell, and the titration port and the detection test paper cooperate with each other.

[0011] According to the above technical solution, a frame plate and a positioning top protrusion are integrally formed in the first end head, the frame plate abuts against the lower surface of the culture vessel, and the positioning top protrusion abuts against the top of the culture vessel.

[0012] According to the above technical solution, the temporary positioning groove of the holding body is extended with an inward sliding groove, the top of the inward sliding groove is integrally formed with a positioning convex, and the outer circumference of the collection needle is integrally formed with a limiting convex, and the limiting convex cooperates with the positioning convex.

[0013] According to the above technical solution, a limiting partition is integrally formed on the upper surface of the connecting bridge, and two ends of the limiting partition are respectively connected to the inner side of the first end and the inner side of the second end.

[0014] According to the above technical solution, the distance between the top of the holding body and the lower edge of the limiting ring is greater than the distance between the breaking mark and the tail end of the collecting needle.

[0015] A method for using a dual-sampling zoonotic pathogen rapid detection device includes the following steps:

[0016] Step 1: Collect body fluids from humans and livestock using a collector, i.e., using a collection needle to collect body fluids, including but not limited to blood;

[0017] Step 2: introducing the body fluid sample collected by the collection needle into the incubator, that is, breaking off the tail end of the collection needle so that the body fluid sample can be effectively introduced into the liquid storage tube of the incubator for sample propagation to increase the abundance;

[0018] Step 3: The culture time is limited based on the temperature. When the time is reached, the culture solution with theoretical abundance is led out of the liquid storage tube to the test paper of the detection tester, and the corresponding detection is performed through the virus antigen in the test paper;

[0019] Step 4: Query the test results fed back by the test paper to determine the pathogen.

[0020] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0021] Body fluids of livestock and humans are sampled through the sampling in the collector, including but not limited to blood or saliva. After regular culture in the culture medium in the incubator, the culture medium containing the sampled body fluid is introduced into the detection tester, and the corresponding pathogen antigen detection is carried out through the test paper fixed in the detection tester. The whole detection process is closed, which reduces the influence of external environmental pathogens, greatly shortens the detection time, and improves the convenience of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0023] Figure 1 It is a perspective three-dimensional structural schematic diagram of the present invention;

[0024] Figure 2 It is a three-dimensional structural schematic diagram of the combined joint of the present invention from one viewing angle;

[0025] Figure 3 It is a schematic diagram of the top cross-sectional structure of the combined joint of the present invention;

[0026] Figure 4 is a schematic diagram of the main cross-sectional structure of the first end of the present invention;

[0027] Figure 5 is a schematic diagram of the main cross-sectional structure of the second end of the present invention;

[0028] Figure 6 It is a schematic diagram of the three-dimensional structure of the culture device of the present invention from one viewing angle;

[0029] Figure 7 It is a schematic diagram of the three-dimensional structure of the culture device of the present invention from a second viewing angle;

[0030] Figure 8 It is a schematic diagram of the three-dimensional structure of the collector of the present invention from one viewing angle;

[0031] Fig. 9 is a schematic diagram of a top cross-sectional structure of a collector of the present invention;

[0032] Fig.10 yes Fig. 9 Detailed view of point A in the middle;

[0033] Fig.11 It is a schematic diagram of the three-dimensional structure of the detection tester of the present invention from one viewing angle.

[0034] In the figure: 1, combined body, 101, connecting bridge, 102, first end, 103, second end, 104, limiting partition, 105, placement groove, 106, push opening, 107, exposed opening, 108, panel, 109, positioning top protrusion, 110, guide pipe;

[0035] 2. incubator, 201. liquid storage tube, 202. liquid injection tube, 203. rubber sealing plug, 204. liquid outlet tube, 205. one-way valve, 206. piston;

[0036] 3. Collector, 301. Holding body, 302. Combination cap, 303. Accommodating groove, 304. Collecting needle, 305. Limiting ring, 306. Breaking mark, 307. Limiting convex, 308. Positioning convex, 309. Inward sliding groove;

[0037] 4. Detection tester, 401. housing, 402. titration port, 403. detection test paper. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] See also Figure 1-11 The present invention provides a technical solution: a dual-sampling rapid detection device for zoonotic pathogens, including a combination body 1, an incubator 2, a collector 3 and a detection tester 4, the incubator 2 is fixedly installed at both ends of the combination body 1, the detection tester 4 is fixedly installed in the middle of the combination body 1, and the collector 3 is embedded on the upper surface of both ends of the combination body 1.

[0040] The combined unit 1 is the basic structure for combined installation of the device, the incubator 2 cultures pathogens in the collected body fluid samples, the collector 3 collects body fluid samples from humans and animals, and the detection tester 4 is made based on the principle of antigen test paper and is redesigned and adjusted for overall structural matching.

[0041] Specifically, the combined body 1 includes a connecting bridge 101, a first end 102 and a second end 103, the first end 102 and the second end 103 are respectively integrally formed at the two ends of the connecting bridge 101, the first end 102 and the second end 103 are centrally symmetrical structures, the upper surface of the first end 102 is integrally formed with a placement groove 105, the placement groove 105 is embedded with a collector 3, the upper shell of the first end 102 is provided with an exposed port 107, the exposed port 107 is located at the head end of the placement groove 105, the wall surface of the rear end of the placement groove 105 is provided with a push port 106, the culture vessel 2 is fixedly installed in conjunction with the exposed port 107 and the push port 106, a guide tube 110 is fixedly installed in the first end 102, one end of the guide tube 110 is matched with the liquid outlet of the culture vessel 2, and the other end of the guide tube 110 cooperates with the dripping end of the detection tester 4.

[0042] The main structure is formed by connecting the carrier bridge 101, the first end 102 and the second end 103. The carrier bridge 101 supports the detection tester 4 and is limited by the first end 102 and the second end 103. The placement groove 105 provides a placement position for the sampler. The exposed port 107 and the push port 106 are used in conjunction with the incubator 2. The design of the guide tube 110 facilitates the effective guidance of the mixed liquid in the incubator 2 to the detection tester 4.

[0043] Specifically, the culture vessel 2 includes a liquid storage tube 201, an injection tube 202 and a liquid outlet tube 204. A piston 206 is slidably installed in the liquid storage tube 201 and is filled with culture fluid. The injection tube 202 is integrally formed on the upper surface of the liquid storage tube 201. A sealing rubber plug 203 is installed in the injection tube 202. The sealing rubber plug 203 cooperates with the exposed port 107. The liquid outlet tube 204 is integrally formed on the lower surface of the liquid storage tube 201. A one-way valve 205 is fixedly installed in the liquid outlet tube 204. The liquid outlet tube 204 is consistent with one end of the guide tube 110.

[0044] The liquid outlet tube 204 cooperates with the piston 206 to form a closed storage space for the culture fluid, the injection tube 202 cooperates with the sealing rubber plug 203 to form a closed structure at the injection end, and it is convenient for the collector 3 to introduce the collected sample body fluid into the culture fluid. The liquid outlet tube 204 cooperates with the one-way valve 205 to form a one-way closed structure at the liquid outlet end, so that the mixed liquid after culture can be effectively discharged and the backflow problem can be avoided.

[0045] Specifically, the collector 3 includes a holding body 301, a combination cap 302 and a collecting needle 304. A temporary positioning groove is provided in the holding body 301, and the collecting needle 304 is clamped in the temporary positioning groove. The combination cap 302 is inserted into the head of the holding body 301. The combination cap 302 is provided with a receiving groove 303. The head of the collecting needle 304 is located in the receiving groove 303. A limiting ring 305 is integrally formed at the head of the collecting needle 304, and a breaking mark 306 is provided at the tail of the collecting needle 304.

[0046] The holding body 301, the combination cap 302 and the collection needle 304 are the main structures of the collector 3. The temporary positioning groove allows the collection needle 304 to be temporarily positioned when not in use. After sampling, the limiting ring 305 cooperates to allow the tail of the collection needle 304 to slide out of the holding body 301. The design of the breaking mark 306 allows the collection needle 304 to be easily broken after the tail end slides out, so that the collected sample body fluid can be effectively introduced into the incubator 2. The combination cap 302 has a receiving groove 303, so that it can effectively accommodate the head of the collection needle 304 after being combined with the holding body 301, thereby preventing the head of the collection needle 304 from being damaged.

[0047] Specifically, the detection tester 4 includes a shell 401 , a titration port 402 is provided on the upper surface of the head of the shell 401 , a detection test paper 403 is fixedly installed in the shell 401 , and the titration port 402 and the detection test paper 403 cooperate with each other.

[0048] The housing 401 provides a molding foundation for the titration port 402 , and the test paper 403 is an antigen test paper, which performs antigen sensing detection on the introduced culture fluid containing the sample body fluid.

[0049] Specifically, a frame plate 108 and a positioning protrusion 109 are integrally formed in the first end head 102 , the frame plate 108 abuts against the lower surface of the culture vessel 2 , and the positioning protrusion 109 abuts against the top of the culture vessel 2 .

[0050] By setting the frame 108 and the positioning top protrusion 109, the position of the culture container 2 can be effectively fixed to avoid position changes that affect its use.

[0051] Specifically, the temporary positioning groove of the holding body 301 is extended with an inward sliding groove 309, and the top of the inward sliding groove 309 is integrally formed with a positioning protrusion 308. The outer periphery of the collection needle 304 is integrally formed with a limiting protrusion 307, and the limiting protrusion 307 cooperates with the positioning protrusion 308.

[0052] The retracted slide groove 309 cooperates with the positioning protrusion 308 to form a temporary locking structure. At the same time, based on the setting of the limit protrusion 307, the initial position of the sampling needle is fixed, ensuring the convenience and practicality of sampling, and effectively protecting the sampling needle.

[0053] Specifically, a limiting partition plate 104 is integrally formed on the upper surface of the connecting bridge 101 , and two ends of the limiting partition plate 104 are respectively connected to the inner side of the first end 102 and the inner side of the second end 103 .

[0054] By setting the limiting partition 104, the detection testers 4 on both sides will not be squeezed and collided, and cross infection caused by excessive liquid flow can be avoided.

[0055] Specifically, the distance between the top of the holding body 301 and the lower edge of the limiting ring 305 is greater than the distance between the breaking mark 306 and the tail end of the collecting needle 304 .

[0056] By designing the relative size difference between the two, the breaking mark 306 can be effectively exposed, making breaking easier.

[0057] A method for using a dual-sampling zoonotic pathogen rapid detection device includes the following steps:

[0058] Step 1: Collect body fluids from humans and livestock through the collector 3, i.e., collect through the collection needle 304, including but not limited to blood;

[0059] Step 2: introduce the body fluid sample collected by the collection needle 304 into the incubator 2, that is, break off the tail end of the collection needle 304, so that the body fluid sample is effectively introduced into the liquid storage tube 201 of the incubator 2 for sample propagation to increase the abundance;

[0060] Step 3: The culture time is limited based on the temperature. When the time is reached, the culture solution with theoretical abundance is led out of the liquid storage tube 201 to the test paper 403 of the detection tester 4, and the corresponding detection is performed through the virus antigen in the test paper 403;

[0061] Step 4: query the test result fed back by the test paper 403 to determine the pathogen.

[0062] Working principle: When in use, after taking the device out of the sealed sterile bag, remove the collector 3, remove the combination cap 302, and sample the body fluids of humans and livestock respectively through the siphon principle of the collection needle 304, and then pierce the sealing rubber plug 203 with the collection needle 304, continue to press down so that the limiting ring 305 produces a limiting effect, and finally disengage the temporary lock of the collection needle 304 and the holding body 301, and then slide the tail of the collection needle 304 out, and break the tail of the collection needle 304 through the break mark 306, and introduce the siphoned sample body fluid into the liquid storage tube 201 to form a mixed culture solution. After a period of culture, the mixed culture solution in the culture vessel 2 is guided to the detection tester 4 through the collector 3, and the results are effectively displayed through the detection tester 4.

[0063] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0064] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A dual-sampling zoonotic pathogen rapid detection device, comprising a combined body (1), a culture device (2), a collector (3) and a detection tester (4), characterized in that: Culture vessels (2) are fixedly installed in the two ends of the combined body (1), a detection tester (4) is fixedly installed in the middle of the combined body (1), and collectors (3) are embedded on the upper surfaces of the two ends of the combined body (1).

2. A dual sampling zoonotic pathogen rapid detection device according to claim 1, characterized in that: The combined body (1) comprises a connecting bridge (101), a first end head (102) and a second end head (103); the first end head (102) and the second end head (103) are respectively integrally formed at two ends of the connecting bridge (101); the first end head (102) and the second end head (103) are centrally symmetrical structures; a placement groove (105) is integrally formed on the upper surface of the first end head (102); a collector (3) is embedded in the placement groove (105); and the upper shell of the first end head (102) An exposed opening (107) is provided, and the exposed opening (107) is located at the head end of the placement groove (105). A push opening (106) is provided on the wall surface of the tail end of the placement groove (105). The culture vessel (2) is fixedly installed in conjunction with the exposed opening (107) and the push opening (106). A guide tube (110) is fixedly installed inside the first end head (102). One end of the guide tube (110) is matched with the liquid outlet of the culture vessel (2), and the other end of the guide tube (110) is matched with the dripping end of the detection tester (4).

3. A dual sampling zoonotic pathogen rapid detection device according to claim 2, characterized in that: The culture vessel (2) comprises a liquid storage tube (201), an injection tube (202) and a liquid outlet tube (204); a piston (206) is slidably mounted in the liquid storage tube (201) and is filled with culture fluid; the injection tube (202) is integrally formed on the upper surface of the liquid storage tube (201); a rubber sealing plug (203) is installed in the injection tube (202); the rubber sealing plug (203) cooperates with the exposed port (107); the liquid outlet tube (204) is integrally formed on the lower surface of the liquid storage tube (201); a one-way valve (205) is fixedly mounted in the liquid outlet tube (204); the liquid outlet tube (204) and one end of the guide tube (110) are mutually matched.

4. A dual sampling zoonosis rapid detection device according to claim 1, characterized in that: The collector (3) comprises a holding body (301), a combination cap (302) and a collection needle (304); a temporary positioning groove is provided in the holding body (301), the collection needle (304) is clamped in the temporary positioning groove, the combination cap (302) is inserted into the head of the holding body (301), the combination cap (302) is provided with a receiving groove (303), the head of the collection needle (304) is located in the receiving groove (303), a limiting ring (305) is integrally formed at the head of the collection needle (304), and a breaking mark (306) is provided at the tail of the collection needle (304).

5. The dual sampling zoonotic pathogen rapid detection device according to claim 1, characterized in that: The detection tester (4) comprises a shell (401), a titration port (402) is provided on the upper surface of the head of the shell (401), a detection test paper (403) is fixedly installed in the shell (401), and the titration port (402) and the detection test paper (403) cooperate with each other.

6. A dual sampling zoonotic pathogen rapid detection device according to claim 2, characterized in that: A support plate (108) and a positioning top protrusion (109) are integrally formed in the first end head (102); the support plate (108) abuts against the lower surface of the culture vessel (2), and the positioning top protrusion (109) abuts against the top of the culture vessel (2).

7. A dual sampling zoonotic pathogen rapid detection device according to claim 4, characterized in that: The temporary positioning groove of the holding body (301) is extended with an inward sliding groove (309), and the top of the inward sliding groove (309) is integrally formed with a positioning clamping protrusion (308). The outer periphery of the collection needle (304) is integrally formed with a limiting clamping protrusion (307), and the limiting clamping protrusion (307) cooperates with the positioning clamping protrusion (308).

8. A dual sampling zoonotic pathogen rapid detection device according to claim 2, characterized in that: A limiting partition (104) is integrally formed on the upper surface of the connecting bridge (101), and two ends of the limiting partition (104) are respectively connected to the inner side of the first end (102) and the inner side of the second end (103).

9. A dual sampling zoonotic pathogen rapid detection device according to claim 7, characterized in that: The distance between the top of the holding body (301) and the lower edge of the limiting ring (305) is greater than the distance between the breaking mark (306) and the tail end of the collecting needle (304).

10. A method for using a dual-sampling zoonotic pathogen rapid detection device according to claims 1-9, characterized in that: The following steps are involved: Step 1: Collecting body fluids from humans and livestock using a collector (3), i.e., collecting body fluids using a collection needle (304), including but not limited to blood; Step 2: introducing the body fluid sample collected by the collection needle (304) into the incubator (2), i.e. breaking off the tail end of the collection needle (304) so ​​that the body fluid sample can be effectively introduced into the liquid storage tube (201) of the incubator (2) for sample propagation to increase the abundance; Step 3: The culture time is limited based on the temperature. When the time is reached, the culture solution with theoretical abundance is led out of the liquid storage tube (201) to the test paper (403) of the detection tester (4), and the corresponding detection is performed through the virus antigen in the test paper (403); Step 4: query the test result fed back by the test strip (403) to determine the pathogen.

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