Pleurotus nebrodensis strain as well as sporocarp and application thereof

Abstract

The invention discloses a pleurotus nebrodensis strain, a sporocarp of the pleurotus nebrodensis strain and application of the sporocarp of the pleurotus nebrodensis strain. The preservation number of the pleurotus nebrodensis strain is GDMCC No: 64080. The strain is high in environmental adaptability, different mushroom body forms can be cultivated in different cultivation environments, the mushroom shapes can be regulated and controlled according to market requirements, and large-area popularization of pleurotus nebrodensis is promoted. According to the method, the limitation that the pleurotus nebrodensis can only produce the primordium under low-temperature stimulation is eliminated, the primordium can be produced at the normal temperature of 18-25 DEG C after hyphae are full, and the cultivation technical difficulty is reduced. According to the method, the cultivation period is shortened to 33-38 days, the production cost is saved, and mass production becomes possible. According to the pleurotus nebrodensis cultivation method, the fruiting rate reaches 100%, the biological conversion rate reaches 90-100%, and the economic efficiency of pleurotus nebrodensis cultivation and the production enthusiasm of farmers are greatly improved.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and more specifically to a Pleurotus nebrodensis strain, a fruiting body thereof and applications thereof. Background Art

[0002] The existing white mushroom (Pleurotus eryngii var.tuoliensis) has the following defects: (1) White mushroom has poor temperature adaptability and high requirements for cultivation conditions. It needs low temperature stimulation and temperature change to accelerate budding, and cannot be promoted on a large scale. White mushroom is cultured at a temperature of about 24°C. After 40 to 50 days, it will grow all over the cultivation material and enter the post-ripening stage. The temperature is lowered to 20 to 22°C to allow it to continue to grow and accumulate nutrients. After another 30 to 35 days of cultivation, low temperature stimulation is carried out. Stimulation is carried out at a low temperature of 0 to 3°C for 7 to 14 days, or the temperature is kept at 0 to 15°C, with a temperature difference of 10 to 15°C between day and night, and stimulation is carried out for 10 to 15 days. (2) The entire production cycle is long, requiring 90 to 100 days. (3) The fruiting rate of Pleurotus eryngii is only 60-70%, and the bioconversion rate is only 40%. The fruiting rate is low. The existing patent has a fruiting rate of 100%, but the conversion rate is only 80%, which causes a waste of resources, makes the economic efficiency of cultivating Pleurotus eryngii low, and affects the production enthusiasm of growers. (4) The resources of wild Pleurotus eryngii are limited, and it cannot form a yield. The cultivation cost is high and cannot be promoted to ordinary people. (5) The common Pleurotus eryngii has a stipe length of 3-10 cm and a cap length of 5-15 cm. The morphological characteristics are short stipes, large caps, and a single mushroom body shape. Therefore, it is urgent to cultivate new varieties of Pleurotus eryngii with good environmental adaptability, low requirements for cultivation conditions, short production cycle, high fruiting rate and high bioconversion rate, and diversified mushroom body morphology. Summary of the invention

[0003] In order to solve the above technical problems, the present invention utilizes hybrid breeding technology to carry out single-single hybridization breeding of Xinghe Bailing Mushroom No. 1 and Bailing Mushroom, and obtains a new variety of Bailing Mushroom - Xinghe Bailing Mushroom No. 2, which has strong environmental adaptability, low requirements for cultivation conditions, short production cycle, high mushroom yield, high bioconversion rate, and diversified mushroom body morphology.

[0004] The first aspect of the present invention provides a Pleurotus eryngii var.tuoliensis strain, Its deposit number is GDMCC No:64080.

[0005] A second aspect of the present invention provides a Pleurotus nebrodensis fruiting body, which is obtained by cultivating the Pleurotus nebrodensis strain described in the first aspect of the present invention.

[0006] In a preferred embodiment, the cap length of the fruiting body is 3 to 15 cm, for example, 4 cm, 5 cm, 6 cm, 7 cm, 8 cm, 9 cm, 10 cm, 11 cm, 12 cm, 13 cm, 14 cm or 15 cm.

[0007] In a preferred embodiment, the stipe length of the fruiting body is 3 to 20 cm, for example 4 cm, 5 cm, 6 cm, 7 cm, 8 cm, 9 cm, 10 cm, 11 cm, 12 cm, 13 cm, 14 cm, 15 cm, 16 cm, 17 cm, 18 cm, 19 cm or 20 cm.

[0008] In a preferred embodiment, the cap is off-white with stripes.

[0009] A third aspect of the present invention provides a Pleurotus nebrodensis mycelium, which is obtained by culturing the Pleurotus nebrodensis strain described in the first aspect of the present invention.

[0010] A fourth aspect of the present invention provides a Pleurotus nebrodensis bacterial liquid, which is obtained by culturing the Pleurotus nebrodensis strain described in the first aspect of the present invention.

[0011] Another aspect of the present invention further provides a method for culturing the Pleurotus eryngii fruiting body as described in the second aspect of the present invention, comprising culturing and cultivating the Pleurotus eryngii strain on a cultivation medium.

[0012] Another aspect of the present invention provides a method for culturing the Pleurotus nebrodensis mycelium as described in the third aspect of the present invention or the Pleurotus nebrodensis bacterial liquid as described in the fourth aspect of the present invention, comprising culturing the Pleurotus nebrodensis strain in a liquid culture medium or a solid culture medium.

[0013] Another aspect of the present invention also provides the use of the Pleurotus nebrodensis strain described in the first aspect of the present invention in preparing any one of Pleurotus nebrodensis spores, Pleurotus nebrodensis mycelium, Pleurotus nebrodensis liquid and Pleurotus nebrodensis fruiting bodies.

[0014] Another aspect of the present invention also provides the use of the Pleurotus eryngii fruiting body as described in the second aspect of the present invention in food processing.

[0015] On the other hand, the present invention also provides the use of the Pleurotus eryngii strain as described in the first aspect of the present invention, the Pleurotus eryngii fruiting body as described in the second aspect of the present invention, the Pleurotus eryngii mycelium as described in the third aspect of the present invention, or the Pleurotus eryngii liquid as described in the fourth aspect of the present invention in the preparation of any one of immunity enhancing drugs, cholesterol lowering drugs, anti-aging drugs, analgesic drugs, sedative drugs, cough and phlegm-relieving drugs, laxative and detoxification drugs and blood pressure lowering drugs.

[0016] On the basis of being in accordance with the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0017] The reagents and raw materials used in the present invention are commercially available.

[0018] The positive and progressive effects of the present invention are:

[0019] (1) The present invention provides a new Pleurotus eryngii strain, which has strong adaptability to the environment and can cultivate different mushroom body morphologies in different cultivation environments. The mushroom shape can be regulated according to market demand, thereby promoting the large-scale promotion of Pleurotus eryngii.

[0020] (2) The present invention eliminates the limitation that Pleurotus nebrodensis can only produce primordia under low temperature stimulation. After the mycelium is fully grown, primordia can be produced at a normal temperature of 18 to 25° C., thereby reducing the difficulty of cultivation technology.

[0021] (3) The cultivation cycle of the present invention is as short as 33 to 38 days, which saves production costs and makes mass production possible.

[0022] (4) The present invention has a mushroom yield rate of 100% and a bioconversion rate of 90-100%, which greatly improves the economic efficiency of Pleurotus nebrodensis cultivation and the production enthusiasm of growers.

[0023] Biomaterial Deposit Information

[0024] The Pleurotus eryngii var.tuoliensis strain of the present invention was deposited in the Guangdong Microbiological Culture Collection Center (GDMCC) on November 28, 2023. The deposit address is: 5th Floor, Building 59, Compound Experimental Building, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, and the deposit number is: GDMCC No: 64080. The culture name is Pleurotus eryngii var.tuoliensis. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a photo of the fruiting body of Xinghe Bailing Mushroom No. 2.

[0026] Figure 2 This is a photo of the fruiting body of Xinghe Bailing Mushroom No. 1, the parent of Xinghe Bailing Mushroom No. 2.

[0027] Figure 3 This is a photo of the fruiting body of the parent white mushroom of Xinghe White Mushroom No. 2.

[0028] Figure 4 It is a schematic diagram of antagonistic reaction, where No. 1 represents Xinghe Bailing Mushroom No. 1, and No. 2 represents Xinghe Bailing Mushroom No. 2.

[0029] Figure 5 It is the technical route for bacterial strain identification and detection.

[0030] Figure 6 This is a schematic diagram of the normal temperature fruiting of Xinghe Bailing Mushroom No. 2 and its parent Bailing Mushroom.

[0031] Figure 7 This is a schematic diagram of the fruiting of Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii. DETAILED DESCRIPTION

[0032] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. The experimental methods in the following examples without specifying specific conditions are selected according to conventional methods and conditions, or according to the product specifications. The data collection and analysis methods of the following examples are as follows:

[0033] Example 1 Origin process of Xinghe Bailing Mushroom No. 2

[0034] (1) Parent selection: Xinghe Bailing Mushroom No. 1 and Bailing Mushroom were selected as parents. Xinghe Bailing Mushroom No. 1 is a new variety developed by Guangdong Xinghe Edible Fungi Technology Research Co., Ltd. It has been deposited in the Guangdong Microbial Culture Collection Center on November 28, 2023, with a deposit number of GDMCC No: 64079. Bailing Mushroom is the fruiting body of Kitajima Bailing Mushroom purchased by the inventor in Japan in December 2018. The parent strain Bailing Mushroom strain with good mushroom production was obtained through fruiting body tissue separation.

[0035] (2) Single spore collection: Select fruiting bodies with good mushroom shape and caps about to open, cut the caps with gills from the edge of the cap to the place where the stipe is attached, hang them in a sterile and dry triangular flask, and culture them in the dark for 1 to 3 days at a temperature of 20 to 22° C. and a relative humidity of 60 to 70%. After white spore powder imprints are produced, spore dilution and plate coating operations are performed. The coated plates are cultured at a constant temperature and in the dark at a temperature of 20 to 22° C. and a relative humidity of 60 to 70%. The temperature is kept at 20 to 22° C. and the relative humidity is 60 to 70%. The single spores are collected. When the daughter hyphae grow to 0.5-1 cm, plate transfer culture is carried out, and the culture is carried out at a constant temperature and light-proof at a temperature of 20-22° C. and a relative air humidity of 60-70%. The culture is carried out for 10-20 days, and an advantageous plate with fast growth is selected for test tube transfer. At the same time, the hyphae of the plate that has completed the transfer is examined under a microscope. If there is no lock-like joint structure under the microscope, it is a single spore. The single spore test tube continues to be cultured at a constant temperature and light-proof at a temperature of 20-22° C. and a relative air humidity of 60-70%. After the hyphae cover the inclined surface, they are preserved at a storage temperature of 4-10° C.

[0036] (3) Single-spore hybridization: Single-spore hybridization of Xinghe Bailing Mushroom No. 1 and single-spore hybridization of Bailing Mushroom Yes, cut and activate The edge of the single spore colony was inoculated into the plate, and the two inoculation blocks were placed 3 cm apart. At 1.5 cm from the center of the plate, with the mycelium facing upward, constant temperature and dark-proof culture were carried out at 20-22° C. and relative air humidity of 60-70%. After the mycelium of two monospore colonies came into contact, the culture was continued in the dark for 5-10 days.

[0037] (4) Hybrid strains: The hyphae at the junction of two monosporic colonies were selected for microscopic examination, and the plates with lock-like joint structures were transferred to the plates with the hyphae at the junction. The plates were cultured at a constant temperature and in the dark at a temperature of 20-22°C and a relative humidity of 60-70%. The culture was carried out for 10-15 days, and the dominant plates with fast growth were selected for test tube transfer. At the same time, the hyphae of the plates that have completed the transfer were examined under a microscopic examination. The hybrid strains with lock-like joint structures under the microscopic examination were continued to be cultured in the test tubes at a constant temperature and in the dark at a temperature of 20-22°C and a relative humidity of 60-70%. After the hyphae have covered the slope, they were preserved at a storage temperature of 4-10°C.

[0038] (5) Cultivation and screening: The hybrid strain obtained was subjected to cultivation experiments and screening according to conventional methods. After fruiting test, a new variety of Pleurotus eryngii, Xinghe Pleurotus eryngii No. 2, was obtained, which has strong environmental adaptability, low requirements for cultivation conditions, short production cycle, high fruiting rate, high bioconversion rate, and diversified mushroom body morphology.

[0039] Example 2 Morphological characteristics and biological properties

[0040] (1) Morphological characteristics: The fruiting body photo of Xinghe Bailing Mushroom No. 2 is as follows Figure 1 As shown, when the fruiting body of Xinghe Bailing Mushroom No. 2 matures, the stipe is white, subcentrally or laterally, rod-shaped, and 3 to 20 cm long. The gills are off-white, the cap is off-white with spots, the flesh of the cap is hard, the cross-section is fan-shaped or lateral to fan-shaped, 3 to 15 cm long, 3 to 15 cm wide, and 2 to 6 cm thick. The fruiting body photos of Xinghe Bailing Mushroom No. 2, the parent of Xinghe Bailing Mushroom No. 1 and Bailing Mushroom, are shown in Figure 2 , Figure 3 ,Depend on Figure 1 , Figure 2 , Figure 3 It can be seen that the color and pattern of the cap of Xinghe Bailing Mushroom No. 2 are obviously different from those of Xinghe Bailing Mushroom No. 1 and Bailing Mushroom, which shows that the Xinghe Bailing Mushroom No. 2 strain of the present invention is a new strain that is different from its parent in terms of morphological characteristics.

[0041] (2) Biological characteristics

[0042] Nutrition: The main nutrients needed are carbohydrates, proteins, cellulose, hemicellulose, vitamins, minerals, etc.

[0043] Temperature: The mycelium culture temperature is 20-25℃, and the mushroom cultivation temperature is 15-25℃.

[0044] Light: Mycelium does not require light, and the light intensity required during the fruiting period is 0 to 800 lux.

[0045] Moisture: relative humidity of air for mycelium culture is 60-70%, moisture content of mycelium culture medium is 60-65%, The relative humidity of the air in the mushroom cultivation room is 50-90%.

[0046] Air: Good ventilation and fresh air are required, with a carbon dioxide concentration of 1000-5000ppm.

[0047] Time requirement: It usually takes 33 to 60 days from cultivation inoculation to completion of mushroom production.

[0048] Example 3 Antagonistic Response

[0049] Testing basis: Inoculation, cultivation and observation of samples to be tested are carried out in accordance with the agricultural industry standard of the People's Republic of China "NY / T1845-2010 Differential Identification and Antagonistic Reaction of Edible Fungi Species".

[0050] The Xinghe Pleurotus eryngii No. 2 strain obtained in Example 1 was subjected to an antagonistic reaction with its parent Xinghe Pleurotus eryngii No. 1 strain and Pleurotus eryngii strain.

[0051] (1) Culture medium: PDA medium (potato dextrose agar medium), 200 g potato (with extract), 20 g glucose, 20 g agar, 1000 ml water, natural pH, introduced into a culture dish and cooled into a flat plate.

[0052] (2) Inoculation and culture: The inoculation combinations were 5 pairs, Xinghe Bailing Mushroom No. 1 and Xinghe Bailing Mushroom No. 1, Bailing Mushroom and Bailing Mushroom, Xinghe Bailing Mushroom No. 2 and Xinghe Bailing Mushroom No. 2, Xinghe Bailing Mushroom No. 2 and Xinghe Bailing Mushroom No. 1, and Xinghe Bailing Mushroom No. 2 and Bailing Mushroom. Strictly follow the aseptic operation, cut the 0.2 cm × 0.2 cm inoculation block from the edge of the activated bacterial colony with an inoculation shovel, inoculate one inoculation block each time, with a 3 cm interval between the two inoculation blocks, and place them 1.5 cm from the center of the plate, with the mycelium facing upwards, with 3 replicates in each group, and culture them in confrontation, at a temperature of 24-26°C, with ventilation and light protection for 14 days.

[0053] (3) Observation and judgment of antagonistic reactions

[0054] Under the light, observe the hyphae reaction at the junction of the two strains on the culture surface. The antagonistic reaction results are shown in Figure 4 . The mycelium on the culture surfaces of Xinghe Bailing Mushroom No. 1 and Xinghe Bailing Mushroom No. 1, Bailing Mushroom and Bailing Mushroom, Xinghe Bailing Mushroom No. 2 and Xinghe Bailing Mushroom No. 2 did not show any of the three reactions of bulge type, groove type and isolation type, which means that Xinghe Bailing Mushroom No. 1, Bailing Mushroom and Xinghe Bailing Mushroom No. 2 will not show antagonistic reactions themselves. The mycelium on the culture surfaces of Xinghe Bailing Mushroom No. 2 and Xinghe Bailing Mushroom No. 1, Xinghe Bailing Mushroom No. 2 and Bailing Mushroom showed isolation type and bulge type reactions respectively, and there were antagonistic reactions, which means that Xinghe Bailing Mushroom No. 2, Xinghe Bailing Mushroom No. 1 and Bailing Mushroom are different varieties.

[0055] Example 4 Bacterial species identification and detection

[0056] The whole genomes of Xinghe Bailing Mushroom No. 2, its parent Xinghe Bailing Mushroom No. 1, yellow Bailing Mushroom, and its parent Bailing Mushroom were sequenced, and the sequencing results were compared with the Bailing Mushroom genome on NCBI (GENE ID NO is ASM3687298v1). The yellow Bailing Mushroom is the fruiting body of the yellow Bailing Mushroom purchased by the inventor in Japan in December 2018. The yellow Bailing Mushroom strain with good mushroom production was obtained through fruiting body tissue separation.

[0057] (1) Experimental instruments, reagents, and consumables are shown in Table 1.

[0058] Table 1 Main instruments, reagents and consumables

[0059]

[0060] (2) Technical route

[0061] Technical route such as Figure 5 shown.

[0062] (3) Genome comparison results

[0063] The sequencing results of Xinghe Bailing Mushroom No. 2, parent Xinghe Bailing Mushroom No. 1, yellow Bailing Mushroom, and parent Bailing Mushroom were compared with the Bailing Mushroom genome on NCBI. The results are shown in Table 2. The comparison found that the comparison rates of all samples were between 81.39% and 93.87%, all higher than 75% and the data had large differences, indicating that the four samples all belonged to the Bailing Mushroom species, but did not belong to the same strain. The genome comparison results directly indicate that the Xinghe Bailing Mushroom No. 2 strain of the present invention belongs to the Bailing Mushroom species, and is a new strain different from the parent Xinghe Bailing Mushroom No. 1, yellow Bailing Mushroom, and parent Bailing Mushroom.

[0064] Table 2 Genome alignment

[0065]

[0066]

[0067] Note: (1) Sample: sample name.

[0068] (2) Mapped reads: The number of reads mapped to the reference (including single-end and double-end alignments).

[0069] (3)Total reads: The total number of reads of valid sequencing data.

[0070] (4) Mapping rate: the number of reads mapped to the reference genome divided by the number of reads in the effective sequencing data.

[0071] (5) Average depth: Average sequencing depth, the total number of bases aligned to the reference genome divided by the size of the genome covered.

[0072] (6) Coverage at least 1X: The percentage of sites in the reference genome that are covered by at least 1 base.

[0073] (7) Coverage at least 4X: The percentage of sites in the genome that are covered by at least 4 bases in the reference genome.

[0074] Example 5 Cultivation Experiment

[0075] Xinghe Bailing Mushroom No. 2 and its parents Xinghe Bailing Mushroom No. 1 and Bailing Mushroom were cultivated in bottles to produce mushrooms.

[0076] (1) Plate culture: The bacteria were inoculated on a PDA plate and cultured at a constant temperature of 20-25° C. and a relative humidity of 60-70% for 10-15 days to obtain plate bacteria.

[0077] (2) Liquid primary seed culture: The plate strain obtained in step (1) is inoculated into a triangular flask liquid culture medium and cultured in the dark at a temperature of 20 to 25° C. for 5 to 10 days to obtain a liquid primary seed. The triangular flask liquid culture medium is formulated as follows: 5% glucose, 5% white sugar, 0.5% soybean meal, 0.5% peptone, 0.2% magnesium sulfate, 0.2% potassium dihydrogen phosphate, and natural pH.

[0078] (3) Liquid secondary seed culture: The liquid primary seed obtained in step (2) is inoculated into a fermentation tank liquid culture medium and cultured in the dark at a temperature of 20 to 25° C. for 5 to 10 days to obtain a liquid secondary seed. The formula of the fermentation tank liquid culture medium is the same as that of the flask liquid culture medium.

[0079] (4) Inoculation and culture: The liquid secondary seed is inoculated into the culture medium of the culture bottle and cultured in the dark, the culture temperature is 20-25°C, the relative humidity of the air is 60-70%, the carbon dioxide concentration is 2000-3000ppm, and the mycelium is fully grown in the culture bottle after 40 days of culture. The culture medium of the culture bottle is formulated as follows: 21% corn cobs, 20% sawdust, 19% cottonseed hulls, 19% bran, 16% rice bran, 5% corn flour, 64.5% water content, and 530g material weight.

[0080] (5) Management of mushroom production: Open the bottle mouth, remove the mushroom skin on the surface of the material, and transfer it to the mushroom room. The culture temperature is 15-25°C, the relative humidity of the air is 70-90%, and the carbon dioxide concentration is 1000-3000ppm. The light intensity is set to 0-100 lux in the first 1-7 days, and then set to 200-800 lux. Turn on the shelf lights intermittently every day. After 16-23 days of cultivation, the fruiting body caps are fully expanded, but the edges of the caps are still slightly curled, and then harvested.

[0081] The results of the bottle cultivation experiment of Xinghe Bailing Mushroom No. 2 and its parents Xinghe Bailing Mushroom No. 1 and Bailing Mushroom are shown in Table 3. The fruiting body photos are shown in Figure 1 , Figure 2 , Figure 3 As shown in Table 3, under the same culture cycle and control parameters, the total production cycle and fruiting rate of Xinghe Bailing Mushroom No. 2 are the same as those of Xinghe Bailing Mushroom No. 1, but the bioconversion rate is 4% higher than that of Xinghe Bailing Mushroom No. 1. Bailing Mushroom has the longest total production cycle, the lowest fruiting rate and bioconversion rate. Figure 1 , Figure 2 , Figure 3 It can be seen that the cap colors and patterns of the three mushrooms are different. This shows that the Xinghe Bailing Mushroom No. 2 strain of the present invention is a new strain different from the parent, has obvious advantages in mushroom yield rate and bioconversion rate, and has a short cultivation period.

[0082] Table 3 Cultivation experimental results of Xinghe Bailing Mushroom No. 2 and its parents

[0083]

[0084] Example 6 Cultivation Experiment

[0085] After the mycelium of Xinghe Bailing Mushroom No. 2 and the parent Bailing Mushroom in the bottle culture is fully grown, the mushrooms are fruited at a room temperature of 18-25℃.

[0086] (1) Obtain liquid secondary seeds of Xinghe Bailing Mushroom No. 2 and its parent Bailing Mushroom in the manner of Example 5.

[0087] (2) Inoculation and culture: The liquid secondary seed is inoculated into the culture medium of the culture bottle and cultured in the dark, the culture temperature is 20-25°C, the relative humidity of the air is 60-70%, the carbon dioxide concentration is 2000-3000ppm, and the mycelium grows all over the culture bottle for 20 days. The culture medium of the culture bottle is formulated as follows: 21% corn cobs, 20% sawdust, 19% cottonseed hulls, 19% bran, 16% rice bran, 5% corn flour, 64.5% water content, and 530g material weight.

[0088] (3) Management of mushroom production: Open the bottle mouth and transfer to the mushroom production room. Fruit the mushrooms at room temperature of 18-25℃, relative humidity of 60-80%, carbon dioxide concentration of 1000-2000ppm, turn on the shelf lights intermittently every day, and set the light intensity to 100-200 lux. Cultivate for 13-23 days. Harvest when the fruiting body cap is fully expanded but the edge of the cap is still slightly curled.

[0089] The experimental results of the fruiting of Xinghe Bailing Mushroom No. 2 and the parent Bailing Mushroom at room temperature of 18-25℃ after the mycelium of the bottle culture was fully grown are shown in Table 4, and the fruiting body photos are shown in Figure 6 As shown in Table 4, the total production cycle of Xinghe Bailing Mushroom No. 2 is 10 days shorter than that of its parent Bailing Mushroom, the fruiting rate is 81% higher, and the bioconversion rate is 78% higher. Figure 6 It can be seen that the mushroom body of Xinghe Bailing Mushroom No. 2 is Bailing Mushroom, the stipe is white, eccentric, rod-shaped, 3-4 cm long, and the gills and cap are off-white. This shows that the Xinghe Bailing Mushroom No. 2 of the present invention has strong adaptability to the environment, can produce mushrooms at a normal temperature of 18-25°C, and has obvious advantages over the parent Bailing Mushroom in production cycle, fruiting rate, and bioconversion rate.

[0090] Table 4 Experimental results of Xinghe Bailing Mushroom No. 2 and its parent Bailing Mushroom cultivated at normal temperature

[0091]

[0092] Example 7 Cultivation Experiment

[0093] Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii were grown in a bag culture method. Pleurotus eryngii is the fruiting body of Pleurotus eryngii brought back by the inventor from Guangdong Muhe Ecological Agriculture Development Co., Ltd. in December 2021. The Pleurotus eryngii strain with good fruiting was obtained through fruiting body tissue separation.

[0094] (1) Obtain liquid secondary seeds of Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii in the manner of Example 5.

[0095] (2) Inoculation and culture: The liquid secondary seed is inoculated into the bag culture medium and cultured in the dark, the culture temperature is 20-25°C, the relative humidity of the air is 60-70%, the carbon dioxide concentration is 2000-3000ppm, and the mycelium is fully grown in the bag after 40 days of culture. The bag culture medium formula is 18% corn cobs, 43% miscellaneous sawdust, 19% bran, 10% rice bran, 10% corn flour, 65% water content, and the material weight is 1500g.

[0096] (3) Management of mushroom production: Open the bag and transfer it to the mushroom production room. The culture temperature is 15-20°C, the relative humidity is 50-70%, and the carbon dioxide concentration is 3000-5000ppm. The light intensity is set to 150-200 lux for the first 10 days, and then to 10-100 lux. Turn on the top light intermittently every day. After 20 days of cultivation, the fruiting body caps are fully expanded, but the edges of the caps are still slightly curled, so as to harvest.

[0097] The experimental results of Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii in the bag cultivation method are shown in Table 5, and the fruiting body photos are shown in Figure 7 As shown in Table 5, the total production cycle and fruiting rate of Xinghe Bailing Mushroom No. 2 are the same as those of Pleurotus eryngii, but its bioconversion rate is 19% higher than that of Pleurotus eryngii. Figure 7 It can be seen that the fruiting bodies of Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii have similar morphological characteristics, with white, rod-shaped, 14-18 cm long stipes and 4-6 cm long caps, but the gills and caps of the two mushrooms are very different in color, with the gills and caps of Xinghe Bailing Mushroom No. 2 being off-white, while the gills and caps of Pleurotus eryngii are brown. This shows that the Xinghe Bailing Mushroom No. 2 of the present invention has the advantage of diversified mushroom body morphology, and the mushroom shape can be regulated according to market demand, thereby broadening the audience group of Pleurotus eryngii and promoting the market penetration of Pleurotus eryngii.

[0098] Table 5 Cultivation experimental results of Xinghe Bailing Mushroom No. 2 and Pleurotus eryngii

[0099]

Claims

1. A Pleurotus eryngii var. tuoliensis strain, whose deposit number is GDMCC No: 64080.

2. A Pleurotus eryngii fruiting body, characterized in that: The Pleurotus nebrodensis fruiting body is obtained by cultivating the Pleurotus nebrodensis strain according to claim 1.

3. The Pleurotus eryngii fruiting body according to claim 2, characterized in that The cap of the fruiting body is 3 to 15 cm long, and / or the stipe is 3 to 20 cm long, and / or the cap is off-white with stripes.

4. A Pleurotus nebrodensis mycelium, characterized in that: The Pleurotus nebrodensis mycelium is obtained by culturing the Pleurotus nebrodensis strain according to claim 1.

5. A white mushroom liquid, characterized in that: The Pleurotus nebrodensis liquid is obtained by culturing the Pleurotus nebrodensis strain as claimed in claim 1.

6. The method for cultivating the fruiting body of Pleurotus eryngii according to claim 2 or 3, characterized in that: The method comprises culturing and cultivating the Pleurotus nebrodensis strain on a cultivation medium.

7. The method for culturing the Pleurotus nebrodensis mycelium according to claim 4 or the Pleurotus nebrodensis liquid according to claim 5, characterized in that: The method comprises culturing the Pleurotus nebrodensis strain on a liquid culture medium or a solid culture medium to obtain the strain.

8. Use of the Pleurotus nebrodensis strain according to claim 1 in preparing any one of Pleurotus nebrodensis spores, Pleurotus nebrodensis mycelium, Pleurotus nebrodensis liquid and Pleurotus nebrodensis fruiting bodies.

9. Use of the Pleurotus eryngii fruiting body as claimed in claim 2 or 3 in food processing.

10. Use of the Pleurotus eryngii strain according to claim 1, the Pleurotus eryngii fruiting body according to claim 2 or 3, the Pleurotus eryngii mycelium according to claim 4, or the Pleurotus eryngii liquid according to claim 5 in the preparation of any one of immunity enhancing drugs, cholesterol lowering drugs, anti-aging drugs, analgesic drugs, sedative drugs, cough and phlegm-relieving drugs, laxative and detoxifying drugs, and antihypertensive drugs.

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