Acoustic levitation microscopy testing device
By using an acoustic suspension microscopy device, real-time continuous observation of activated sludge particles is achieved through sound wave and airflow propulsion technology, which solves the problem of low efficiency of manual slicing and provides efficient and dynamic observation results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HEILONGJIANG UNIV
- Filing Date
- 2025-01-02
- Publication Date
- 2026-06-09
Smart Images
Figure CN119804237B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of intelligent detection technology, specifically to an acoustic levitation microscopy testing device. Background Technology
[0002] The activated sludge process is one of the most commonly used wastewater treatment methods both domestically and internationally. The structure of activated sludge reflects the condition of the wastewater treatment system. Regularly monitoring the microscopic parameters of activated sludge flocs can provide important information on the dynamic changes in wastewater treatment operating parameters. Therefore, it is necessary to analyze and observe activated sludge flocs in different states.
[0003] However, in most wastewater treatment plants, the observation of activated sludge particles remains only at the theoretical stage. Some large-scale plants require professionals to manually slice the activated sludge particles, which is affected by human factors and is time-consuming, labor-intensive, and inefficient. Furthermore, manual slicing can only achieve static observation by sampling before testing, and cannot achieve real-time continuous observation, thus the results obtained are partial and incomplete. Summary of the Invention
[0004] To address the aforementioned problems of low efficiency and inability to achieve real-time continuous observation in manual sectioning, this invention proposes an acoustic levitation microscopy testing device. This invention utilizes acoustic levitation for stable particle transport, enabling dynamic, real-time, and continuous observation. By employing a cold needle sample processing system for layer-by-layer analysis of activated sludge, it can achieve particle dissection of activated sludge. Similarly, it can be applied to medical pathology sectioning, the dissection of microbial cell structures in biology, and the monitoring of metazoans in ecology.
[0005] This invention proposes an acoustic levitation microscopy testing device, specifically comprising a support, a quantitative sample injection device, an acoustic levitation stabilization device, a protective gas propulsion device, a cold needle sample processing device, a sample preparation device, and an image analysis system. The quantitative sample injection device is located at one end of the support, the acoustic levitation stabilization device is located in the middle, and the cold needle sample processing device is located at the other end. The quantitative sample injection device includes a quantitative jet gun and a jet head. One end of the quantitative jet gun is connected to the support via a telescopic robotic arm, and the other end of the quantitative jet gun is connected to the support via a spring. The jet head is connected to the support via the telescopic robotic arm and is horizontally positioned below the quantitative jet gun, spraying... The air head and air pump are connected; the acoustic levitation stabilization device includes a reflector and a sound wave generator, with the reflector located in the middle of the support and the sound wave generator located directly below the reflector; the nozzle of the quantitative jet gun and the outlet of the jet head are located between the reflector and the sound wave generator; the cold needle sample processing device includes several cold needles, which are mounted on the support via a telescopic robotic arm; a sample preparation device and an image analysis system are located below the cold needles, with the sample preparation device including several reagent kits; the cold needles deliver the collected sample into the reagent kits, which are then transferred to the image analysis system via the sample preparation device for image analysis of the sample.
[0006] Furthermore, the acoustic levitation stabilization device also includes three automatic zoom microscopes and an electromagnetic relay. The electromagnetic relay is located at the bottom of the acoustic generator. The automatic zoom microscopes are located on the acoustic generator and between the acoustic generator and the reflector to observe the droplets dripped from the quantitative jet gun.
[0007] Furthermore, the support is equipped with a light source compensation device, which provides a light source for the automatic zoom microscope.
[0008] Furthermore, the cold needle includes a cold needle plate and several fine needles. The cold needle plate is a regular polygonal ring structure, with two fine needles on each side of the cold needle plate and several fine needles pointing towards the center of the cold needle plate. The cold needle plate is vertically arranged, and its edges are connected to the telescopic robotic arm.
[0009] Furthermore, the side length of the cold needle plate decreases from the direction closer to the sound wave generator to the direction farther away from the sound wave generator.
[0010] Furthermore, the bracket is equipped with a cold needle stabilizing device and a slide rail. The cold needle is slidably mounted on the bracket via a telescopic robotic arm and the slide rail. The cold needle is inserted into the cold needle stabilizing device by extending the telescopic robotic arm, and the temperature of the cold needle is maintained by the low-temperature environment inside the cold needle stabilizing device.
[0011] Furthermore, the sample preparation device also includes a robotic arm, a conveying device, and a telescopic support, with the telescopic support positioned below the conveying device; the robotic arm grips the reagent kit and places it onto the conveying device via the telescopic support.
[0012] Furthermore, a reagent kit storage box is provided at the end of the conveying device.
[0013] Furthermore, the image analysis system includes a microscope and a display, with the microscope positioned on one side of the transmission device and the microscope and display connected together.
[0014] Furthermore, the acoustic levitation microscopy testing device also includes a gas propulsion device, which includes several jet heads. These jet heads are positioned in the middle and at the end between the reflector and the sound wave generator to control the droplet balance.
[0015] The beneficial effects of the acoustic levitation microscopic testing device described in this invention are as follows:
[0016] (1) The acoustic suspension microscopy testing device of the present invention overcomes the problems of low efficiency and inability to achieve real-time continuous observation by manual slicing. The droplet is suspended by the sound wave generator and reflector and comes into contact with several cold needles under the push of the airflow. The cold needles peel off the droplet and send the sample into the reagent kit. The sample in the reagent kit is observed by the microscope. The whole process does not require manual slicing, which effectively improves efficiency. The purpose of continuous observation can be achieved by continuously dripping droplets through the quantitative jet gun. Attached Figure Description
[0017] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.
[0018] In the attached diagram:
[0019] Figure 1 This is a schematic diagram of the structure of an acoustic levitation microscopic testing device according to the present invention;
[0020] Figure 2 This is a schematic diagram of the automatic zoom microscope setup of the acoustic levitation microscope testing device described in this invention.
[0021] Figure 3 This is a schematic diagram of the quantitative jet gun of the acoustic levitation microscopy testing device described in this invention;
[0022] Figure 4 This is a schematic diagram of the structure of the cold needle in the acoustic levitation microscopy testing device described in this invention;
[0023] Figure 5 This is a schematic diagram of the structure of a reagent kit for an acoustic levitation microscopy testing device according to the present invention;
[0024] Among them: 1-air pump, 2-jet head, 3-quantitative jet gun, 4-reflector, 5-telescopic robotic arm, 6-cold needle, 7-automatic zoom microscope, 8-sound wave generator, 9-robotic arm, 10-cold needle stabilizing device, 11-electromagnetic relay, 12-microscope, 13-display, 14-light source compensation device, 15-transfer device, 16-telescopic support, 17-reagent box, 18-spring, 19-reagent, 20-support, 21-slide rail. Detailed Implementation
[0025] The technical solution of this invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only a part of, and not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without inventive effort are within the scope of protection of this invention.
[0026] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.
[0027] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.
[0028] Furthermore, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
[0029] Specific implementation method one: See Figures 1-5This embodiment is described in detail. The acoustic levitation microscopy testing device described in this embodiment specifically includes a support 20, a quantitative sample injection device, an acoustic levitation stabilization device, a cold needle sample processing device, a sample preparation device, and an image analysis system. The support 20 has a quantitative sample injection device at one end, an acoustic levitation stabilization device in the middle, and a cold needle sample processing device at the other end. The quantitative sample injection device includes a quantitative jet gun 3 and a jet head 2. The quantitative jet gun 3 is mounted on the support 20, and one end of the quantitative jet gun 3 is connected to the support 20 via a telescopic robotic arm 5. The telescopic robotic arm 5 can control the movement of the quantitative jet gun 3, with a rotation radius of 25cm-30cm. A spring 18 is also provided on the quantitative jet gun 3, and the spring 18 is connected to the support 20. A tensioning device is provided on the spring 18, which controls the tension of the spring 18, thereby generating different vibration amplitudes. The telescopic robotic arm 5 applies a fixed force to the quantitative jet gun 3 and controls the spring 18 at approximately... Under the beam, the quantitative jet gun 3 vibrates, causing sample droplets to drip from the nozzle of the quantitative jet gun 3. The jet head 2 is connected to the support 20 via the telescopic mechanical arm 5 and is horizontally positioned below the quantitative jet gun 3. The jet head 2 is connected to the air pump 1. The acoustic suspension stabilization device includes a reflector 4 and a sound wave generator 8. The reflector 4 is positioned in the middle of the support 20, and the sound wave generator 8 is positioned directly below the reflector 4. The sound wave generator 8 is used to send sound waves, and the generator 4 is used to receive sound waves to form a sound field. The nozzle of the quantitative jet gun 3 and the outlet of the jet head 2 are located between the reflector 4 and the sound wave generator 8. When the sample is dripped from the nozzle of the quantitative jet gun 3, the sample droplets can be suspended in the sound field under the action of the reflector 4 and the sound wave generator 8. The cold needle sample processing device includes several cold needles 6, which are positioned on the support 20 via the telescopic mechanical arm 5. After the sample is dripped, it moves in the sound field under the action of the gas ejected from the jet head 2 until it contacts the cold needles 6.
[0030] Below several cold needles 6 are a sample preparation device and an image analysis system. The sample preparation device includes several reagent kits 19, which are semi-solid agar culture medium kits. The reagent kits 19 are 25cm long and 10cm wide. The two sides of the reagent kits 19 are magnetic. The reagent kits 19 are divided into several sections. The cold needles 6 deliver the collected samples into the reagent kits 19. The reagent kits 19 are transferred to the image analysis system through the sample preparation device for image analysis of the samples.
[0031] The acoustic levitation stabilization device also includes three automatic zoom microscopes 7 and an electromagnetic relay 11. The electromagnetic relay 11 is located at the bottom of the acoustic wave generator 8 and controls the acoustic wave generator 8. The automatic zoom microscopes 7 are located on the acoustic wave generator 8 and between the acoustic wave generator 8 and the reflector 4. The three automatic zoom microscopes 7 are evenly arranged in a circle to make a preliminary visual judgment on the droplets dripped from the quantitative jet gun 3 from three different angles.
[0032] The support 20 is equipped with a light source compensation device 14, which provides a light source for the automatic zoom microscope 7. The light source compensation device 14 is an LED light source.
[0033] The cold needle 6 includes a cold needle plate and several fine needles. The cold needle plate is a regular polygonal ring structure. In this embodiment, the cold needle plate is a regular hexagonal ring structure. Two fine needles are provided on each side of the cold needle plate, and several fine needles point to the center of the cold needle plate. The cold needle plate is vertically arranged, and the cold needle plates of several cold needles 6 are coaxially arranged. The edge of the cold needle plate is connected to the telescopic robotic arm 5.
[0034] The side length of the cold needle plate decreases from the direction closer to the sound wave generator 8 to the direction farther away from the sound wave generator 8. That is, the cold needle plate size is larger for the cold needles 6 closer to the sound wave generator 8 and smaller for the cold needles 6 farther away from the sound wave generator 8. In this embodiment, the side lengths of the cold needle plates are 10mm, 8mm, and 6mm respectively. The needles on the different cold needles 6 are of the same length, so that the circular structure formed by the ends of the needles at the center of the cold needle 6 gradually shrinks towards the direction farther away from the sound wave generator 8.
[0035] The support 20 is equipped with a cold needle stabilizing device 10 and a slide rail 21. One end of the cold needle 6 is connected to the telescopic robotic arm 5, and the other end of the telescopic robotic arm 5 is connected to the slide rail 21, so that several cold needles 6 are slidably arranged on the support 20. The inside of the cold needle stabilizing device 10 is a low-temperature environment, which is achieved by setting dry ice or by using a heat exchange medium to continuously cool the inside of the cold needle stabilizing device 10. After the cold needle 6 completes one sample collection, it moves to the top of the cold needle stabilizing device 10 under the action of the slide rail 21. The telescopic robotic arm 5 extends and inserts the cold needle 6 into the cold needle stabilizing device 10. The low-temperature environment inside the cold needle stabilizing device 10 cools the cold needle 6 and maintains the temperature stability of the cold needle 6.
[0036] The sample preparation device also includes a robotic arm 9, a conveying device 15, and a telescopic support 16, with the telescopic support 16 positioned below the conveying device 15. The robotic arm 9 grips the reagent kit 19 and places it onto the conveying device 15 via the telescopic support 16. The conveying device 15 is 2 meters long, 1 meter wide, and operates at a speed ranging from 0.1 mm / s to 0.5 mm / s.
[0037] The end of the conveying device 15 is provided with a reagent kit storage box 17.
[0038] The image analysis system includes a microscope 12 and a display 13. The microscope 12 is located on one side of the conveying device 15 and is connected to the display 13. The microscope 12 performs zoned monitoring of the samples in the reagent kit 19 and displays the results on the display 13. The display 13 can be operated to automatically record video or image data, and then perform deep analysis, classification and other operations through deep learning and other algorithms to form a large dataset, providing a basis for subsequent testing and verification. The obtained high-precision model can provide results for subsequent monitoring.
[0039] The acoustic levitation microscopy testing device also includes a gas propulsion device, which comprises several jet heads 2. These jet heads 2 are positioned at the middle and end of the space between the reflector 4 and the sound wave generator 8, controlling the droplet balance. The jet heads 2 are inclined and divided into two parts: one part is mounted on the support 20, and the other part is positioned on the ground. The two parts of the jet heads 2 are positioned vertically opposite each other, and the angle between the exhaust direction of the two parts of the jet heads and the horizontal plane ranges from 30° to 45°, controlling the parallel movement of the droplets. Figure 1 As shown.
[0040] The specific working process of the acoustic levitation microscopic testing device described in this invention is as follows:
[0041] Before sampling, press and hold the button on the top of the quantitative jet gun 3 to submerge the nozzle of the quantitative jet gun 3 in the liquid. During sampling, slowly release the top button by manipulating the telescopic robotic arm 5 to further transfer the nozzle into the sound field of the acoustic levitation device. The droplet size can be determined by the range of the quantitative jet gun, so the droplet volume range is 100-1000 microliters. After the nozzle is moved into the sound field, the telescopic robotic arm 5 applies a fixed stress to the quantitative jet gun 3, so that the stress can be controlled during sample dripping, thereby controlling the number of samples dripped by the quantitative jet gun. The sample droplets are dripped into the sound field through the cooperation of the telescopic robotic arm 5 and the spring 18.
[0042] After the sample droplet falls into the sound field between the reflector 4 and the sound generator 8, the horizontally positioned jet head 2 provides the moving power for the sample droplet. During the horizontal movement, the sample droplet passes through the automatic zoom microscope 7, which makes a preliminary visual judgment on the sample droplet. Afterward, several tilted jet heads 2 of the gas propulsion device adjust the position of the sample droplet in the sound field, control the parallel movement of the droplet, and ensure that the sample droplet can contact the cold needle 6.
[0043] When the sample droplet passes through the cold needle 6, the extremely low temperature allows for layer-by-layer dissection, causing the dissected liquid to adhere to the cold needle 6 and form a sample. The cold needle plate is then stretched horizontally by the telescopic robotic arm 5, causing the hexagon to unfold into a rectangle; then, the telescopic robotic arm 5 is pressed down, causing it to pierce into the culture medium of the reagent kit 19 held by the robotic arm 9, thus completing the sample processing.
[0044] After sample processing, the telescopic support 16 rises and passes through the conveyor 15, at which point the conveyor 15 stops operating, and the robotic arm 9 places the reagent kit 19 onto the telescopic support 16. The telescopic support 16 then descends, placing the reagent kit 19 onto the conveyor 15, where it is magnetically attached by its two edges. The conveyor 15 is then manipulated to transport the reagent kit 19 to the microscope 12 for zonal monitoring. The observed images are transmitted to the monitor 13, which can be automatically recorded as video or image data. This data is then processed using deep learning algorithms for deep analysis and classification, generating a large dataset to provide a basis for subsequent testing and verification. The resulting high-precision model provides results for subsequent monitoring. After observation, the reagent kit 19 falls into the reagent kit storage box 17 at the end of the conveyor 15.
[0045] In summary, the acoustic levitation microscopy device of the present invention overcomes the problems of low efficiency and inability to achieve real-time continuous observation by manual slicing. The droplet is suspended by the sound wave generator 8 and the reflector 4 and comes into contact with several cold needles 6 under the push of the airflow. The cold needles 6 peel off the droplet and send the sample into the reagent kit 19. The sample in the reagent kit 19 is observed by the microscope 12. The whole process does not require manual slicing, which effectively improves efficiency. The continuous observation can be achieved by continuously dripping droplets through the quantitative jet gun 3.
[0046] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the invention. They can also be reasonable combinations of the features described in the above embodiments. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A microscopic testing device for acoustic levitation, characterized in that: The system includes a support (20), a quantitative injection device, an acoustic levitation stabilization device, a cold needle sample processing device, a sample preparation device, and an image analysis system. The support (20) has a quantitative injection device at one end, an acoustic levitation stabilization device in the middle, and a cold needle sample processing device at the other end. The quantitative injection device includes a quantitative jet gun (3) and a jet head (2). One end of the quantitative jet gun (3) is connected to the support (20) via a telescopic robotic arm (5), and the other end is connected to the support (20) via a spring (18). The jet head (2) is connected to the support (20) via the telescopic robotic arm (5). The jet nozzle (2) is connected to the support (20) and horizontally positioned below the quantitative jet gun (3). The jet nozzle (2) is connected to the air pump (1). The acoustic suspension stabilization device includes a reflector (4) and a sound wave generator (8). The reflector (4) is positioned in the middle of the support (20), and the sound wave generator (8) is positioned directly below the reflector (4). The nozzle of the quantitative jet gun (3) and the outlet of the jet nozzle (2) are located between the reflector (4) and the sound wave generator (8). The cold needle sample processing device includes several cold needles (6). The cold needles (6) are positioned on the support (20) via a telescopic robotic arm (5). A sample preparation device and an image analysis system are provided below several cold needles (6). The sample preparation device includes several reagent kits (19). The cold needles (6) deliver the collected samples into the reagent kits (19), and the reagent kits (19) are transferred to the image analysis system to perform image analysis on the samples. The cold needle (6) includes a cold needle plate and several fine needles. The cold needle plate is a regular polygonal ring structure. Two fine needles are provided on each side of the cold needle plate, and several fine needles point to the center of the cold needle plate. The cold needle plate is vertically arranged, and the cold needle plates of several cold needles (6) are coaxially arranged. The edge of the cold needle plate is connected to the telescopic mechanical arm (5). The side length of the cold needle plate decreases from the direction close to the sound wave generator (8) to the direction away from the sound wave generator (8). The length of the fine needles on different cold needles (6) is consistent, so that the center of the cold needle (6) gradually shrinks from the circular structure formed by the end of the fine needles to the direction away from the sound wave generator (8).
2. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The acoustic levitation stabilization device also includes three automatic zoom microscopes (7) and an electromagnetic relay (11). The electromagnetic relay (11) is located at the bottom of the acoustic generator (8). The automatic zoom microscope (7) is located on the acoustic generator (8) and between the acoustic generator (8) and the reflector (4) to observe the droplets dripped from the quantitative jet gun (3).
3. The acoustic levitation microscopy testing device according to claim 2, characterized in that: The bracket (20) is provided with a light source compensation device (14), which provides a light source for the automatic zoom microscope device (7).
4. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The bracket (20) is provided with a cold needle stabilizing device (10) and a slide rail (21). The cold needle (6) is slidably mounted on the bracket (20) via a telescopic mechanical arm (5) and a slide rail (21). The cold needle (6) is inserted into the cold needle stabilizing device (10) by extending the telescopic mechanical arm (5). The cold needle (6) is kept at a low temperature by the low temperature environment inside the cold needle stabilizing device (10).
5. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The sample preparation device also includes a robotic arm (9), a conveying device (15) and a telescopic support (16), with the telescopic support (16) located below the conveying device (15); the robotic arm (9) clamps the reagent kit (19) and places it onto the conveying device (15) via the telescopic support (16).
6. The acoustic levitation microscopy testing device according to claim 5, characterized in that: The end of the conveying device (15) is provided with a reagent kit storage box (17).
7. The acoustic levitation microscopy testing device according to claim 5, characterized in that: The image analysis system includes a microscope (12) and a display (13). The microscope (12) is located on one side of the transmission device (15), and the microscope (12) and the display (13) are connected.
8. The acoustic levitation microscopy testing device according to claim 1, characterized in that: The acoustic levitation microscopy testing device also includes a gas propulsion device, which includes several jet heads (2). The several jet heads (2) of the gas propulsion device are located in the middle and at the end between the reflector (4) and the sound wave generator (8) to control the droplet balance.