In-vitro culture method and application of red fiber shrimp seaweed root system

Abstract

The invention provides an in-vitro culture method and application of a red fiber shrimp sea grass root system, belongs to the technical field of plant tissue culture, and can solve the technical problem that independent research on physiological states of roots and leaves of a complete plant of hydroponic red fiber shrimp sea grass in a laboratory is difficult to accurately carry out. The in-vitro culture method of the red fiber shrimp seaweed root system comprises the following steps: collecting a healthy red fiber shrimp seaweed plant as an explant, culturing in an incubator, selecting a healthy plant, carrying out first pruning, disinfection and second pruning, inoculating the plant into a culture plate filled with an induction culture medium, and carrying out root system induction culture until a root primordium germinates, so as to obtain the red fiber shrimp seaweed root system. And transferring to a culture plate filled with a subculture medium for in-vitro root culture. The method can be applied to the physiological research of the red fiber shrimp seaweed root system.

Description

Technical Field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an in vitro culture method and application of a red fiber shrimp seaweed root system. Background Art

[0002] Seagrass is a type of submerged plant that lives in seawater and has important ecological service functions. However, the current physiological research on seagrass lags far behind that of terrestrial plants and seaweed. Phyllospadix seagrass is a type of seagrass that grows on rocky coasts and relies on the mucus secreted by its roots to firmly fix on the rocks to resist erosion by wind and waves.

[0003] Red fiber shrimp seaweed (Phyllospadix iwatensis) is the dominant species in the rocky coastal seagrass fields of the Yellow Sea and Bohai Sea in my country. Its roots are thick and strong, and it is an excellent material for studying the physiology of seaweed roots. And because the roots of red fiber shrimp seaweed have the function of absorbing nutrients and fixing on rocks to resist wind and wave erosion, the root system of red fiber shrimp seaweed has important research value. Because the leaves and roots of red fiber shrimp seaweed have the function of absorbing nutrients, it is difficult to accurately study the physiological state of its roots and leaves by hydroponic red fiber shrimp seaweed complete plants in the laboratory. Therefore, in order to ensure the accurate conduct of physiological experiments on red fiber shrimp seaweed, it is necessary to culture its roots and leaves separately. Traditional indoor seawater culture of shrimp seaweed is difficult to meet the requirements for accurate physiological research on its root system. How to culture the roots of red fiber shrimp seaweed in vitro to obtain red fiber shrimp seaweed roots that meet the conditions for physiological research is an urgent problem to be solved. Summary of the invention

[0004] The present invention aims at the technical problem that it is difficult to accurately conduct root physiology research on the complete plant of red fiber shrimp seaweed hydroponically cultured in the laboratory, and proposes an in vitro culture method and application of the root system of red fiber shrimp seaweed. The present invention uses the adventitious root primordium of red fiber shrimp seaweed as an explant, induces its normal growth and development through tissue culture technology, thereby realizing the in vitro culture of the root system of red fiber shrimp seaweed, and providing good materials for the root physiology research.

[0005] In order to achieve the above object, the technical scheme adopted by the present invention is: an in vitro culture method for the root system of red fiber shrimp seaweed, comprising the following steps: collecting healthy red fiber shrimp seaweed plants as explants, selecting healthy plants after culturing in an incubator for first pruning, disinfection, and second pruning, and then inoculating them in a culture plate filled with an induction culture medium for root induction culture until root primordia germinate, and then transferring them to a culture plate filled with a subculture culture medium for in vitro root culture.

[0006] In some embodiments, the incubator culture conditions are: after retaining at least 10 nodes closest to the stem tip and 4 leaves of the stem tip, the explant is placed in a culture bottle filled with natural seawater, cultured in an incubator, the light intensity is 3000 lux, light: dark = 8h: 16h, temperature is 10°C, after culturing for 3 days, the natural seawater is replaced with sterilized natural seawater and continued to be cultured in the incubator, and the sterilized seawater is replaced daily, the incubator temperature is increased by 1°C every 2 days until the incubator temperature is 15°C, and the culture is continued for 7 days. Preferably, the explant is a healthy red fiber shrimp seaweed plant collected in spring when the water temperature is about 10°C.

[0007] In some of the embodiments, the first pruning step includes the following steps: plants that are still healthy after being cultured in an incubator are selected, and the first pruning is performed on an ultra-clean workbench, the leaves are peeled off to expose the stem tip, and only 4 nodes and 4 internodes near the stem tip are left, and there are two clusters of newly emerged adventitious root primordia on the 1st and 2nd nodes near the stem tip.

[0008] In some of the embodiments, the disinfection step includes the following steps: placing the stem tip obtained after the first pruning into a new sterilized culture bottle, rinsing it with sterilized natural seawater for 3-5 times, drying the surface moisture of the stem tip with sterilized filter paper, placing it in a 10% sodium hypochlorite solution for disinfection for 15 minutes, taking it out, and then rinsing it with sterilized natural seawater for 5 times; wherein the rinsing step with sterilized natural seawater and the disinfection step with a 10% sodium hypochlorite solution are both carried out under vacuum filtration conditions.

[0009] In some embodiments, the second pruning step includes the following steps: placing the explant in sterilized natural seawater with 100 μM VC for a second pruning, cutting off the stem tip, and leaving only 2 nodes and 3 internodes near the stem tip.

[0010] In some embodiments, the root induction culture step includes the following steps: inoculating the explants after the second pruning into a six-well culture plate filled with an induction medium, culturing at 15° C. in the dark, inoculating one explant per well, and waiting for root primordia to germinate. The root primordia germinate after 3 weeks of culture.

[0011] In some embodiments, the induction medium is liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 1 mg / L IBA, and 2 mg / L NAA.

[0012] In some embodiments, the in vitro root culture step includes the following steps: after the root primordium germinates, it is transferred to a six-well culture plate containing a subculture medium for culture, and when the root grows to 5 mm, the root is cut off from the stem and cultured further. After 60 days of culture, the root can grow to 20.5 mm.

[0013] In some embodiments, the secondary culture medium is a liquid MS medium supplemented with 200 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA.

[0014] Another aspect of the present invention provides the use of the red fiber shrimp seaweed root system cultured by the in vitro culture method of the red fiber shrimp seaweed root system in the research of root physiology.

[0015] Compared with the prior art, the advantages and positive effects of the present invention are: the present invention uses the adventitious root primordium of red fiber shrimp seaweed as an explant, and induces its normal growth and development through tissue culture technology, thereby realizing the in vitro culture of the root system of red fiber shrimp seaweed, and providing a good material for its root system physiology research. The present invention can greatly reduce the contamination rate of explant surface disinfection by temporarily culturing red fiber shrimp seaweed indoors; the explant surface disinfection step uses a vacuum filter to filter to ensure thorough disinfection; placing the explant in sterilized natural seawater with VC for pruning can prevent the explant from browning during pruning; using a cell culture plate for culturing can reduce the probability of cross-contamination of the explant, save culture medium, and facilitate observation of contamination and replacement of culture medium. DETAILED DESCRIPTION

[0016] In order to be able to understand the characteristics and technical contents of the embodiments of the present invention in more detail, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the embodiments described below are only part of the embodiments of the present invention, rather than all the embodiments of the present invention. In the following technical description, for the convenience of explanation, a full understanding of the disclosed embodiments is provided through multiple details. However, one or more embodiments can still be implemented without these details. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work belong to the scope of protection requested by the present invention.

[0017] The embodiment of the present invention provides an in vitro culture method and application of the root system of red fiber shrimp seaweed. The present invention uses the adventitious root primordium of red fiber shrimp seaweed as an explant, induces its normal growth and development through tissue culture technology, thereby realizing the in vitro culture of the root system of red fiber shrimp seaweed, and providing good materials for its root physiology research.

[0018] The in vitro culture method of the red fiber shrimp seaweed root system of the present invention comprises the following steps:

[0019] Incubator culture: Collect healthy red fiber shrimp seaweed plants when the water temperature is about 10℃ in spring, wash them with seawater and bring them back to the laboratory. Keep at least 10 nodes closest to the stem tip and 4 leaves at the stem tip of the healthy plants, put them in a culture bottle filled with natural seawater, and culture them in an incubator with a light intensity of 3000lux, light:dark=8h:16h, temperature of 10℃. After 3 days of culture, replace the natural seawater with sterilized natural seawater and continue to culture in the incubator. Replace the sterilized seawater every day, and raise the incubator temperature by 1℃ every 2 days until 15℃. Temporarily culturing red fiber shrimp seaweed indoors can greatly reduce the contamination rate of explant surface disinfection.

[0020] Preliminary selection of explants and first pruning: Select healthy plants after culturing in sterile seawater at 15°C for 7 days, and perform the first pruning on a clean bench. Remove the leaves to expose the stem tip, leaving only the 4 nodes and 4 internodes near the stem tip, and there are two clusters of newly emerged adventitious root primordia on the 1st and 2nd nodes near the stem tip.

[0021] Explant disinfection: Place the stem tip with 4 internodes obtained after the first pruning into a new sterilized culture bottle, rinse with sterilized natural seawater for 3-5 times, dry it with sterilized filter paper, place it in a 10% sodium hypochlorite solution for disinfection for 15 minutes, and then rinse it with sterilized natural seawater for 5 times. During disinfection and rinsing, use a vacuum filter to filter to ensure thorough disinfection.

[0022] Second pruning of explants: Place the explants in sterilized natural seawater with 100 μM VC for the second pruning to prevent browning of the explants during pruning. Cut off the stem tip, leaving only 2 nodes and 3 internodes near the stem tip.

[0023] Root induction culture: the explants after the second pruning were inoculated into a six-well culture plate containing an induction medium, and cultured at 15°C in the dark until the root primordium germinated; the induction medium was a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 1 mg / L IBA+2 mg / L NAA. Using a cell culture plate for culture can reduce the chance of cross-contamination of explants, save culture medium, and facilitate observation of contamination and replacement of culture medium;

[0024] In vitro root culture: After the root primordium germinates, it is transferred to the subculture medium and continued to be cultured in a six-well culture plate. When the root grows to 5 mm, the root is cut off from the stem and continued to be cultured; wherein, the subculture medium is a liquid MS medium supplemented with 200 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA.

[0025] In order to more clearly and in detail introduce the in vitro culture method and application of the red fiber shrimp seaweed root system provided by the embodiment of the present invention, it will be described in combination with specific embodiments below.

[0026] Example 1

[0027] The in vitro culture method of the red fiber shrimp seaweed root system of this embodiment comprises the following steps:

[0028] Selection of explants: Healthy red fiber shrimp seaweed plants were collected in spring when the water temperature was about 10°C, washed with seawater and brought back to the laboratory;

[0029] Incubator culture: Keep the 10 nodes closest to the stem tip and the 4 leaves of the stem tip of the healthy plant, put them into a 1000 ml beaker filled with natural seawater, and culture them in a light incubator for 3 days, then replace the natural seawater with sterilized natural seawater and continue to culture in the incubator. Raise the incubator temperature by 1°C every 2 days until 15°C, and replace the sterilized seawater every day. The culture conditions are: light intensity 3000 lux, light: dark = 8h:16h, temperature 10°C;

[0030] Preliminary selection of explants and first pruning: Select healthy plants after culturing in sterile seawater at 15°C for 7 days, and perform the first pruning on a clean bench. Remove the leaves to expose the stem tip, leaving only the 4 nodes and 4 internodes near the stem tip. There are two clusters of newly emerged adventitious root primordia on the 1st and 2nd nodes.

[0031] Explant disinfection: Place the stem tip with 4 internodes after the first pruning into a new sterilized culture bottle, rinse with sterilized natural seawater for 5 times, dry it with sterilized filter paper, place it in a 10% sodium hypochlorite solution for 15 minutes, and then rinse it with sterilized natural seawater for 5 times. A vacuum filter is used for filtration during the disinfection in the 10% sodium hypochlorite solution and the rinsing with sterilized natural seawater.

[0032] Second pruning of explants: The explants were placed in sterilized natural seawater with 100 μM VC for the second pruning, and the stem tip was cut off, leaving only 2 nodes and 3 internodes close to the stem tip;

[0033] Root induction culture: The explants after the second pruning were inoculated into a six-well culture plate containing an induction medium and cultured at 15°C in the dark. Root primordia germinated after about 3 weeks, and the germination rate was 31.2%. The induction medium was a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 1 mg / L IBA+2 mg / L NAA.

[0034] In vitro root culture: After the root primordium germinates, it is transferred to the subculture medium and continued to be cultured in a six-well culture plate. When the root grows to 5 mm, the root is cut from the stem and cultured again. After 60 days, the root can grow to 20.5 mm. The subculture medium is a liquid MS medium supplemented with 200 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA.

[0035] Comparative Example 1

[0036] The difference from Example 1 is that the frequency of replacing the sterilized seawater in the incubator cultivation step of this comparative example is to replace the sterilized seawater once every 2 days. Specifically, the 10 nodes closest to the stem tip and the 4 leaves of the stem tip of the healthy plant are retained and placed in a 1000 ml beaker filled with natural seawater. After culturing in a light incubator for 3 days, the natural seawater is replaced with sterilized natural seawater and continued to be cultured in the incubator. The incubator temperature is increased by 1°C to 15°C every 2 days, and the sterilized seawater is replaced every 2 days. The culture conditions are: light intensity 3000 lux, light: dark = 8h:16h, temperature 10°C.

[0037] Comparative Example 2

[0038] The difference from Example 1 is that the frequency of replacing the sterilized seawater in the incubator cultivation step of this comparative example is to replace the sterilized seawater once every 3 days. Specifically, the healthy plants retain the 10 nodes closest to the stem tip and the 4 leaves at the stem tip, and are placed in a 1000 ml beaker filled with natural seawater. After culturing in a light incubator for 3 days, the natural seawater is replaced with sterilized natural seawater and continued to be cultured in the incubator. The incubator temperature is increased by 1°C to 15°C every 2 days, and the sterilized seawater is replaced every 3 days. The culture conditions are: light intensity 3000 lux, light: dark = 8h:16h, temperature 10°C.

[0039] Comparative Example 3

[0040] The difference from Example 1 is that in the comparative example, in the step of sterilizing the implants, no vacuum filter is used for filtration when the implants are placed in a 10% sodium hypochlorite solution for disinfection and rinsed with sterilized natural seawater. Specifically, the stem tip with 4 internodes after the first pruning is placed in a new sterilized culture bottle, rinsed with sterilized natural seawater 5 times, dried with sterilized filter paper, placed in a 10% sodium hypochlorite solution for disinfection for 15 minutes, and then rinsed with sterilized natural seawater 5 times.

[0041] Comparative Example 4

[0042] The difference from Example 1 is that in the second pruning step of the explants in this comparative example, the explants are placed in sterilized natural seawater with 0 VC addition for the second pruning, the stem tip is cut off, and only 2 nodes and 3 internodes near the stem tip are left.

[0043] Comparative Example 5

[0044] The difference from Example 1 is that in the second pruning step of the explants in this comparative example, the explants were placed in 50 μM VC sterilized natural seawater for the second pruning, and the stem tips were cut off, leaving only 2 nodes and 3 internodes close to the stem tips.

[0045] Comparative Example 6

[0046] The difference from Example 1 is that the induction medium in the root induction culture step of this comparative example is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 5 mg / L NAA. Specifically, the explants after the second pruning are inoculated in a six-well culture plate containing an induction medium and cultured at 15°C in the dark. After about 3 weeks, the root primordia germinate with a germination rate of 31.2%, wherein the induction medium is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 5 mg / L NAA.

[0047] Comparative Example 7

[0048] The difference from Example 1 is that the induction medium in the root induction culture step of this comparative example is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA. Specifically, the explants after the second pruning are inoculated in a six-well culture plate containing an induction medium and cultured at 15°C in the dark. After about 3 weeks, the root primordia germinate with a germination rate of 31.2%, wherein the induction medium is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA.

[0049] Comparative Example 8

[0050] The difference from Example 1 is that the induction medium in the root induction culture step of this comparative example is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 0.5 mg / L NAA. Specifically, the explants after the second pruning are inoculated in a six-well culture plate containing an induction medium and cultured at 15°C in the dark. After about 3 weeks, the root primordia germinate, and the germination rate is 31.2%, wherein the induction medium is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, and 0.5 mg / L NAA.

[0051] Comparative Example 9

[0052] The difference from Example 1 is that the induction medium in the root induction culture step of this comparative example is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 0.2 mg / L IBA, and 2 mg / L NAA. Specifically, the explants after the second pruning are inoculated in a six-well culture plate containing an induction medium and cultured at 15°C in the dark. After about 3 weeks, the root primordia germinate with a germination rate of 31.2%, wherein the induction medium is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 0.2 mg / L IBA, and 2 mg / L NAA.

[0053] Comparative Example 10

[0054] The difference from Example 1 is that the induction medium in the root induction culture step of this comparative example is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 2 mg / L IBA, and 2 mg / L NAA. Specifically, the explants after the second pruning are inoculated in a six-well culture plate containing an induction medium and cultured at 15°C in the dark. After about 3 weeks, the root primordia germinate with a germination rate of 31.2%, wherein the induction medium is a liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 2 mg / L IBA, and 2 mg / L NAA.

[0055] 30 healthy explants were inoculated in Example 1 and Comparative Examples 1-2 respectively and cultured in an incubator. The contamination rates after one week of culture are shown in Table 1.

[0056] Table 1 Contamination rate of explants cultured in an incubator for one week in Example 1 and Comparative Examples 1-2

[0057] Number of explants Contamination rate after 1 week (%) Example 1 30 21.3 Comparative Example 1 30 64.5 Comparative Example 2 30 90.7

[0058] Thirty explants were selected from Example 1 and Comparative Example 3 for disinfection treatment, and the contamination rates are shown in Table 2.

[0059] Table 2 Contamination rate after explant disinfection in Example 1 and Comparative Example 3

[0060] Number of explants Contamination rate (%) Example 1 30 21.3 Comparative Example 3 30 52.7

[0061] 25 explants were selected from Example 1 and Comparative Examples 4-5 for the second pruning. The browning rates after pruning are shown in Table 3.

[0062] Table 3 Browning rate of explants after the second pruning of Example 1 and Comparative Examples 4-5

[0063] Number of explants Browning rate (%) Example 1 25 10.4 Comparative Example 4 25 95.1 Comparative Example 5 25 46.6

[0064] After the explants in Example 1 and Comparative Examples 6-10 were subjected to root induction culture, the induction rates were shown in Table 3.

[0065] Table 4 Root induction culture induction rate of Example 1 and Comparative Examples 6-10

[0066] Induction rate (%) Example 1 31.2 Comparative Example 6 0 Comparative Example 7 3.6 Comparative Example 8 0.9 Comparative Example 9 8.3 Comparative Example 10 16.4

[0067] As can be seen from the above, compared with Example 1, the frequency of replacing sterilized seawater in the incubator culturing step of Comparative Examples 1-2 is lower than that of Example 1, and the contamination rate of the explants is significantly higher than that of Example 1; compared with Example 1, no vacuum filter is used for filtration during disinfection and rinsing in Comparative Example 3, and the contamination rate of the explants is significantly higher than that of Example 1; compared with Example 1, in the second explant pruning step, the sterilized seawater conditions of the explants in Comparative Examples 4-5 are different, the amount of VC added in the sterilized seawater of Comparative Example 4 is 0, and the amount of VC added in the sterilized natural seawater of Comparative Example 5 is 50 μM, both of which are lower than that of Example 1, and the browning rate of the explants is significantly higher than that of Example 1; compared with Example 1, the IBA and NAA in the culture medium of Comparative Examples 6-10 are different, and the root induction rate is significantly lower than that of Example 1. In summary, Example 1 uses the culture method of the present invention to culture the roots of red fiber shrimp seaweed in vitro, which can greatly reduce the contamination rate and browning rate of the explants. The germination rate after root induction culture can reach 31.2%, and the roots can grow to 20.5 mm after in vitro root culture, which provides a good material for the study of root physiology of red fiber shrimp seaweed.

[0068] Finally, it should be noted that the present invention is not limited to the above-mentioned embodiments. The above-mentioned embodiments are only the preferred feasible embodiments of the present invention, and the above-mentioned embodiments are only used to illustrate the technical scheme of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations, modifications, evolutions and improvements made by ordinary technicians in this field to the technical scheme of the present invention should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for in vitro culture of red fiber shrimp seaweed roots, characterized in that: The method comprises the following steps: collecting healthy red fiber shrimp seaweed plants as explants, selecting still healthy plants after culturing in a culture box, performing first pruning, disinfection, and second pruning, and then inoculating them in a culture plate filled with an induction culture medium for root induction culture until root primordia germinate, and then transferring them to a culture plate filled with a subculture culture medium for in vitro root culture.

2. The in vitro culture method of red fiber shrimp seaweed root system according to claim 1, characterized in that: The incubator culture conditions are as follows: after retaining at least 10 nodes closest to the stem tip and 4 leaves at the stem tip, the explants are placed in a culture bottle filled with natural seawater and cultured in an incubator with a light intensity of 3000 lux, light: dark = 8h:16h, and a temperature of 10°C. After culturing for 3 days, the natural seawater is replaced with sterilized natural seawater and continued to be cultured in the incubator. The sterilized seawater is replaced daily, and the incubator temperature is raised by 1°C every 2 days until the incubator temperature reaches 15°C, and the culture is continued for 7 days.

3. The in vitro culture method of red fiber shrimp seaweed root system according to claim 1, characterized in that: The first pruning step includes the following steps: plants that are still healthy after being cultured in the incubator are selected, and the first pruning is performed on an ultra-clean workbench, the leaves are peeled off to expose the stem tip, and only 4 nodes and 4 internodes near the stem tip are left, and there are two clusters of newly emerged adventitious root primordia on the 1st and 2nd nodes near the stem tip.

4. The in vitro culture method of red fiber shrimp seaweed root system according to claim 1, characterized in that: The disinfection step comprises the following steps: placing the stem tip obtained after the first pruning into a new sterilized culture bottle, washing it with sterilized natural seawater for 3-5 times, drying the surface moisture of the stem tip with sterilized filter paper, placing it in a 10% sodium hypochlorite solution for disinfection for 15 minutes, taking it out, and then washing it with sterilized natural seawater for 5 times; wherein the washing step with sterilized natural seawater and the disinfection step with the 10% sodium hypochlorite solution are both carried out under the condition of vacuum filtration.

5. The in vitro culture method of red fiber shrimp seaweed root system according to claim 1, characterized in that: The second pruning step includes the following steps: placing the explant in sterilized natural seawater with 100 μM VC for the second pruning, cutting off the stem tip, and leaving only 2 nodes and 3 internodes close to the stem tip.

6. The in vitro culture method of red fiber shrimp seaweed root system according to claim 1, characterized in that: The root induction culture step comprises the following steps: inoculating the explants after the second pruning into a six-well culture plate filled with an induction culture medium, and culturing them at 15° C. in the dark until the root primordium germinates.

7. The in vitro culture method of red fiber shrimp seaweed root system according to claim 6, characterized in that: The induction medium was liquid MS medium supplemented with 1000 mg / L hydrolyzed casein, 30 g / L sucrose, 1 mg / L IBA, and 2 mg / L NAA.

8. The in vitro culture method of red fiber shrimp seaweed root system according to claim 6, characterized in that: The in vitro root culture step includes the following steps: after the root primordium germinates, it is transferred to a six-well culture plate filled with a subculture medium for culture, and when the root grows to 5 mm, the root is cut off from the stem and cultured further.

9. The in vitro culture method of red fiber shrimp seaweed root system according to claim 8, characterized in that: The secondary culture medium was liquid MS medium supplemented with 200 mg / L hydrolyzed casein, 30 g / L sucrose, and 2 mg / L NAA.

10. Use of the red fiber shrimp seaweed roots cultured by the in vitro culture method of the red fiber shrimp seaweed roots according to any one of claims 1 to 9 in root physiology research.