Method for dispersing ampullaria gigas egg masses by using enzyme

Abstract

The invention discloses a method for dispersing pomacea canaliculata egg masses by using enzyme, and relates to the field of prevention and control of alien invasive species, a plurality of hydrolase powder with different components are weighed and placed in a plurality of beakers I in a one-to-one correspondence manner, the same amount of distilled water is correspondingly added into the plurality of beakers I respectively, and stirring is performed until the hydrolase powder in each beaker I is completely dissolved; secondly, filtering and sterilizing the hydrolase solution dissolved in each beaker I by using a microporous filter membrane to obtain multiple parts of sterile hydrolase solution, filling the multiple parts of sterile hydrolase solution into sterile bottles in a one-to-one correspondence manner to obtain multiple parts of hydrolase reagents with different concentrations, and storing the hydrolase reagents in a low-temperature refrigerator for later use; according to the method for dispersing the ampullaria gigas egg masses by using the enzyme, the safe and high-applicability hydrolase is used for dispersing the ampullaria gigas eggs and adjusting the structural characteristics of protein of protective mucus covering the surfaces of the eggs, so that cured mucosa is dissolved from the surfaces of egg shells and does not react with the ampullaria gigas eggs, and the ampullaria gigas egg masses are efficiently and completely dispersed.

Description

Technical Field

[0001] The invention relates to a technology for preventing and controlling alien invasive species, and in particular to a method for dispersing egg masses of Pomacea canaliculata using enzymes. Background Art

[0002] Biological invasions not only bring huge losses to the human economy, but also have adverse effects on the ecological environment of the invaded areas. As a malignant invasive alien species, the golden apple snail has not only caused serious economic losses in the invaded areas, but also posed a great threat to local biodiversity. At present, the main means of prevention and control of golden apple snails is to prevent and control adult snails in water bodies, which is difficult to prevent and control the harm of golden apple snails. Therefore, new prevention and control methods must be considered, such as prevention and control of golden apple snail egg masses. Compared with the prevention and control of snail bodies, prevention and control of egg masses has the following advantages: First, even if the prevention and control effect is good, the population of golden apple snails will recover quickly due to the neglect of egg prevention and control. The comprehensive prevention and control of snail bodies and eggs, while reducing the base number of snail bodies, also causes a great blow to the number of snail offspring, and the population is difficult to recover in a short time. The effect of multiple cycles of comprehensive prevention and control of snail eggs will be more obvious. Since there are often 200 to 500 eggs in a nest, the parents of the golden apple snail have to invest a lot, so the efficiency of controlling the egg mass is significantly higher than that of the snail body; secondly, the eggs of the golden apple snail are generally laid on plants, stones, and even cement walls of ditches 15 to 30 cm above the water surface, which are difficult for aquatic organisms to prey on. Controlling the egg mass is targeted and less harmful to the environment than controlling the snail body. When controlling the snail body, the snailicide is directly applied to the water body, which not only kills the golden apple snail, but also has adverse effects on other aquatic organisms, crops, soil, and water sources; while the application of the drug to the egg is more targeted than applying the drug to the water body, the snail-killing effect is better, and the impact on the environment is significantly lower. Therefore, controlling the golden apple snail by targeting the egg mass of the golden apple snail is a more efficient and environmentally friendly way.

[0003] In recent years, the research on Pomacea canaliculata eggs has attracted much attention, involving population, genetics, development and risk assessment. However, it is difficult to separate the adhered eggs intactly by traditional mechanical separation methods. Pomacea canaliculata secretes colloid mucus when laying eggs, which forms a film after drying to fix and protect the eggs, and the egg shell is stable calcium carbonate with strong stress resistance. In the Chinese invention patent with publication number CN104823882A, a method for dispersing Pomacea canaliculata egg masses is disclosed. The method for dispersing Pomacea canaliculata egg masses is to place the Pomacea canaliculata egg masses to be dispersed in a container, add a dispersant and shake well; oscillate or shake the container until the Pomacea canaliculata egg masses are completely dispersed into egg particles; remove the dispersant and debris in the container, and leave the dispersed egg particles in the container; add a detergent to the container, and then wash the egg particles with clean water; add a buffer solution to the container to soak the egg particles; add a detergent to wash the egg particles and wash away the buffer solution; dry or dry the egg particles to obtain dispersed Pomacea canaliculata egg particles.

[0004] When the existing method of dispersing the egg masses of Pomacea canaliculata is used, the dispersant used is mainly a strong alkaline reagent solution such as KOH, NaOH or Ca(OH)2, which is not friendly to the environment. The residual reagent has a certain toxic effect on the eggs of Pomacea canaliculata. In severe cases, it will also affect the normal physiological functions of other aquatic organisms, interfere with the reproduction and growth of aquatic plants and microorganisms, and affect the balance of the entire ecosystem; strong alkali may also cause harm to the human body through skin contact, so there is a certain safety risk for the test operator. Studies have shown that the transparent protective mucus covering the surface of the egg mass of Pomacea canaliculata can be dissolved in water, and there is a color reaction in the biuret reagent, indicating that it contains protein components, and there is no color reaction in the Bial reagent, indicating that there is no sugar component; therefore, it is particularly important to find a safe and environmentally friendly dispersant to denature the protein. The protease in the hydrolase can adjust the protein structure characteristics of the protective mucus covering the surface of the Pomacea canaliculata egg, enhance the dispersibility and solubility of the mucus through surface modification, dissolve the solidified mucus membrane from the surface of the egg shell, retain the integrity of the egg particles, and achieve the purpose of separating the egg particles of Pomacea canaliculata. Summary of the invention

[0005] The invention aims to provide a method for dispersing egg masses of Pomacea canaliculata using enzymes, so as to solve the shortcomings of the prior art that the dispersant is unfriendly to the environment, poses certain safety risks to the test operator and is complicated to operate.

[0006] In order to achieve the above object, the present invention provides the following technical solution: a method for dispersing Pomacea canaliculata egg masses using enzymes, comprising the following steps:

[0007] S1. Weigh a plurality of hydrolase powders of different weights and place them one by one in a plurality of beakers, and add an equal amount of distilled water to each of the plurality of beakers, stir until the hydrolase powder in each beaker is completely dissolved, and then use a microporous filter membrane to filter and sterilize the dissolved hydrolase solution in each beaker to obtain a plurality of sterile hydrolase solutions, and then put the plurality of sterile hydrolase solutions into sterile bottles one by one to obtain a plurality of hydrolase reagents of different concentrations, and store them in a low-temperature refrigerator for later use;

[0008] S2, take a plurality of beakers 2, weigh a plurality of egg masses of equal weight and place them in a plurality of beakers 2 one by one, and add an equal amount of the hydrolase reagents of different concentrations obtained in S1 to a plurality of beakers 2 one by one, shake each beaker 2 evenly, seal each beaker 2 with a sealing film to prevent the solution from splashing out, and shake each beaker 2 overnight;

[0009] S3, the mixed solution containing enzyme and egg grains in each beaker 2 is poured into the sieve respectively in turn for cleaning and filtering, the enzyme reagent remaining on the surface of the egg grains is rinsed with a cleaning agent, and multiple dispersed egg grains are obtained by filtering;

[0010] S4. Place the multiple dispersed egg particles in multiple glass crystallization dishes one by one, and dry the egg particles in each glass crystallization dish. Then, count the number of the dried egg particles in each glass crystallization dish, and finally obtain the number parameters of the egg particles dispersed in each beaker.

[0011] Furthermore, the hydrolase powder in S1 is any one of trypsin, pectinase and pepsin.

[0012] Furthermore, the mass ratio of the hydrolase powder to the distilled water in the S1 is 0.1-1:100; and the concentration of the hydrolase reagent in the S1 is 0.1-1%.

[0013] Furthermore, the pore size range of the microporous filter membrane in the S1 is 0.22 to 1 μm; and the low-temperature storage temperature of the hydrolase reagent in the S1 is set to -20°C to 4°C.

[0014] Furthermore, the mass ratio of the egg mass to the hydrolase reagent in S2 is 0.1-1:1-20.

[0015] Furthermore, the shaking temperature in S2 is set to 25° C. to 30° C.; the shaking overnight time is set to 6 to 18 hours; and the shaking machine speed is set to 100 to 500 rpm.

[0016] Furthermore, the mesh size of the sieve in S3 is set to 140-200 meshes.

[0017] Furthermore, the cleaning agent in S3 is any one of tap water, distilled water and deionized water.

[0018] Furthermore, the drying temperature in S4 is set to 20-30°C.

[0019] Compared with the prior art, the present invention provides a method for dispersing the egg masses of Pomacea canaliculata using enzymes, which uses a hydrolase that is relatively friendly to the aquatic environment, safe and highly applicable to disperse the Pomacea canaliculata, wherein the protease can adjust the protein structure characteristics of the protective mucus covering the surface of the Pomacea canaliculata egg, enhance the dispersion and dissolution ability of the mucus, and dissolve the solidified mucous membrane from the egg shell surface without reacting with the Pomacea canaliculata egg, thereby ensuring the integrity of the Pomacea canaliculata egg particles, and successfully solving the problem that the Pomacea canaliculata egg masses are difficult to disperse efficiently and completely. The method can disperse the Pomacea canaliculata egg masses evenly in a relatively short time, and the operation is relatively simple, and the protective effect of the Pomacea canaliculata egg particles during the dispersion process is better, and the morphological structural integrity of the egg particles themselves is retained, which is convenient for subsequent research on biological functional characteristics. In addition, the dispersant raw materials used in the method provided by the present invention are simple, the composition is stable, and it is easy to produce and prepare, and it also has the advantages of being resistant to storage and transportation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0021] Figure 1 A state diagram of Pomacea canaliculata eggs before and after the egg masses of Pomacea canaliculata are dispersed by the method of the present invention;

[0022] Figure 2 A schematic flow chart of a method for dispersing Pomacea canaliculata egg masses using enzymes provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0023] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0024] Trypsin (Phygene, 1:250), product number PH9033, is white or off-white powder; easily soluble in water; protein content 90-100%; activity 250U / mg.

[0025] Pectinase (Phygene, 500u / mg), product number PH1561, is yellow-brown powder; easily soluble in water; molecular weight 20-60kD; activity ≥500u / mg.

[0026] Pepsin (Phygene, 1:10000), product number PH1492, is an off-white or light yellow powder; easily soluble in water; activity 800-2500u / mg.

[0027] See also Figure 2 A method for dispersing Pomacea canaliculata egg masses using enzymes comprises the following steps:

[0028] S1. Weigh a plurality of hydrolase powders of different weights and place them one by one in a plurality of beakers, and add an equal amount of distilled water to each of the plurality of beakers, stir until the hydrolase powder in each beaker is completely dissolved, and then use a microporous filter membrane to filter and sterilize the dissolved hydrolase solution in each beaker to obtain a plurality of sterile hydrolase solutions, and then put the plurality of sterile hydrolase solutions into sterile bottles one by one to obtain a plurality of hydrolase reagents of different concentrations, and store them in a low-temperature refrigerator for later use; the hydrolase powder is any one of trypsin, pectinase, and pepsin; the mass ratio of the hydrolase powder to distilled water is 0.1 to 1:100; the pore size range of the microporous filter membrane is 0.22 to 1 μm; the low-temperature storage temperature of the hydrolase reagent is set to -20°C to 4°C.

[0029] The specific implementation method is as follows: weigh multiple portions of 0.1-1g hydrolase powder and place them one by one in multiple beakers, and add 100ml of distilled water to each of the multiple beakers, stir until the hydrolase powder in each beaker is completely dissolved, and then use a 0.22-1μm microporous filter membrane to filter and sterilize the dissolved hydrolase solution in each beaker to obtain multiple sterile hydrolase solutions, and then fill the multiple sterile hydrolase solutions into sterile bottles one by one, and divide the filtered and sterilized hydrolase solutions into sterile bottles to obtain a hydrolase reagent with a mass concentration of 0.1-1%, and store it in a -20℃~4℃ refrigerator for standby use.

[0030] S2. Take a plurality of beakers 2 separately, weigh a plurality of egg masses of equal weight and place them one by one in the plurality of beakers 2, and add an equal amount of the hydrolase reagent with a mass concentration of 0.1-1% obtained in S1 to the plurality of beakers 2 one by one, and shake each beaker 2 evenly, and then seal the mouth of each beaker 2 with a sealing film to prevent the solution from splashing out, and shake each beaker 2 overnight to fully disperse the egg masses; the shaking temperature is set to 25°C-30°C; the shaking overnight time is set to 6-18h; the shaking machine speed is set to 100-500rmp; the number of beakers is set to 1-50.

[0031] The specific implementation method is to take a plurality of beakers 2, weigh a plurality of egg masses of equal weight and place them in the plurality of beakers 2 one by one, and add 30 ml of S1 in the plurality of beakers 2 with a mass concentration of 0.1-1%, and shake each beaker 2 evenly, and then seal the mouth of each beaker 2 with a sealing film to prevent the solution from splashing out, and shake each beaker 2 overnight for 12 hours to fully disperse the egg masses; the mass ratio of the egg mass to the hydrolase reagent is 1:1-20;

[0032] The rotation speed when shaking the egg mass and the hydrolase reagent in the beaker can be, but is not limited to, 100 rpm, 200 rpm, 300 rpm, 400 rpm, or 500 rpm.

[0033] S3, pour the mixed solution containing enzyme and egg particles in each beaker into the sieve for cleaning and filtering, rinse the enzyme reagent remaining on the surface of the egg particles with a cleaning agent, and filter to obtain multiple dispersed egg particles; the mesh number of the sieve is set to 140-200 mesh; the cleaning agent is any one of tap water, distilled water, and deionized water.

[0034] S4. Place multiple dispersed egg particles in multiple glass crystallization dishes one by one, and dry the egg particles in each glass crystallization dish. Then, count the number of dried egg particles in each glass crystallization dish, and finally obtain the number parameters of the egg particles dispersed in each beaker. The drying temperature is set to 20-30°C.

[0035] Comparative Example:

[0036] See also Figure 2 A method for dispersing Pomacea canaliculata egg masses using enzymes comprises the following steps:

[0037] (1) Weigh 2 g of egg masses and place them in three beakers 2 respectively, and add 30 ml of distilled water to each beaker 2 respectively. Shake each beaker 2 evenly, and then seal the mouth of the beaker 2 with a sealing film to prevent the reagent from splashing out. Shake each beaker 2 overnight for 12 h to fully disperse the egg masses; the shaking temperature is set to 28°C; the shaking machine speed is set to 200 rpm.

[0038] (2) Pour the mixed solution containing the egg particles in each beaker into a 200-mesh sieve in turn, rinse the residual solution on the surface of the egg particles with tap water, and filter to obtain 3 portions of dispersed egg particles.

[0039] (3) The three dispersed eggs were placed in three glass crystallization dishes one by one, and the eggs in each glass crystallization dish were dried at 30°C. Then, the number of eggs dried in each glass crystallization dish was counted, and finally the number parameters of the eggs dispersed in each beaker were obtained.

[0040] Embodiment 1:

[0041] See also Figure 2 A method for dispersing Pomacea canaliculata egg masses using enzymes comprises the following steps:

[0042] (1) Weigh 0.125 g, 0.25 g, 0.5 g, and 1 g of trypsin powder respectively and place them in 4 beakers, and add 100 ml of distilled water to each of the 4 beakers, stir until the trypsin powder in each beaker is completely dissolved, and then use a 0.22 μm microporous filter membrane to filter and sterilize the dissolved trypsin solution in each beaker to obtain 4 portions of sterile trypsin solution, and then fill the 4 portions of sterile trypsin solution into 4 sterile bottles to obtain trypsin reagents with mass concentrations of 0.125%, 0.25%, 0.5%, and 1%, and store them in a 4°C refrigerator for later use.

[0043] (2) Take another 12 beakers 2, weigh 2 g of egg masses and place them in the 12 beakers 2 respectively. Divide the 12 beakers 2 each containing 2 g of egg masses into three groups. Then, add 30 ml of trypsin reagent with a mass concentration of 0.125%, 0.25%, 0.5%, and 1% to the 4 beakers 2 in each group respectively. Shake each beaker 2 evenly, seal the mouth of each beaker 2 with a sealing film to prevent the trypsin reagent from splashing out, and shake each beaker 2 overnight for 12 hours to fully disperse the egg masses. Set the shaking temperature to 28°C and the speed to 200 rpm.

[0044] (3) pouring the mixed solution containing enzyme and egg particles in each beaker into a 200-mesh sieve for cleaning and filtration, washing the trypsin reagent remaining on the surface of the egg particles with tap water, and filtering to obtain 12 dispersed egg particles;

[0045] (4) The 12 dispersed eggs are placed one by one in 12 glass crystallization dishes, and the eggs in each glass crystallization dish are dried at 30°C. Then, the number of dried eggs in each glass crystallization dish is counted, and finally the number parameters of the eggs dispersed in each beaker are obtained.

[0046] Embodiment 2:

[0047] See also Figure 2 This embodiment provides a technical solution based on Embodiment 1, including the following steps:

[0048] (1) Weigh 0.125 g, 0.25 g, 0.5 g, and 1 g of pectinase powder respectively and place them in 4 beakers, and add 100 ml of distilled water to each of the 4 beakers, stir until the pectinase powder in each beaker is completely dissolved, and then use a 0.22 μm microporous filter membrane to filter and sterilize the dissolved pectinase solution in each beaker to obtain 4 portions of sterile pectinase solution, and then fill the 4 portions of sterile pectinase solution into 4 sterile bottles to obtain pectinase reagents with mass concentrations of 0.125%, 0.25%, 0.5%, and 1%, and store them in a 4°C refrigerator for later use.

[0049] (2) Take another 12 beakers 2, weigh 2 g of egg masses and place them in 12 beakers 2 respectively. Divide the 12 beakers 2 each containing 2 g of egg masses into three groups. Then, add 30 ml of pectinase reagent with a mass concentration of 0.125%, 0.25%, 0.5%, and 1% to each of the four beakers 2 in each group. Shake each beaker 2 evenly, seal the mouth of each beaker 2 with a sealing film to prevent the pectinase reagent from splashing out, and shake each beaker 2 overnight for 12 hours to fully disperse the egg masses. Set the shaking temperature to 28°C and the speed to 200 rpm.

[0050] (3) pouring the mixed solution containing enzyme and egg particles in each beaker into a 200-mesh sieve for cleaning and filtration, rinsing the pectinase reagent remaining on the surface of the egg particles with tap water, and filtering to obtain 12 dispersed egg particles;

[0051] (4) The 12 dispersed eggs are placed one by one in 12 glass crystallization dishes, and the eggs in each glass crystallization dish are dried at 30°C. Then, the number of dried eggs in each glass crystallization dish is counted, and finally the number parameters of the eggs dispersed in each beaker are obtained.

[0052] Embodiment 3:

[0053] See also Figure 2 This embodiment provides a technical solution based on Embodiment 1, including the following steps:

[0054] (1) Weigh 0.125 g, 0.25 g, 0.5 g, and 1 g of pepsin powder respectively and place them in 4 beakers, and add 100 ml of distilled water to each of the 4 beakers, stir until the pepsin powder in each beaker is completely dissolved, and then use a 0.22 μm microporous filter membrane to filter and sterilize the dissolved pepsin solution in each beaker to obtain 4 portions of sterile pepsin solution, and then fill the 4 portions of sterile pepsin solution into 4 sterile bottles to obtain pepsin reagents with mass concentrations of 0.125%, 0.25%, 0.5%, and 1%, and store them in a refrigerator at 4°C for later use.

[0055] (2) Take another 12 beakers 2, weigh 2 g of egg masses and place them in the 12 beakers 2 respectively. Divide the 12 beakers 2 each containing 2 g of egg masses into three groups. Then, add 30 ml of pepsin reagent with a mass concentration of 0.125%, 0.25%, 0.5%, and 1% to the 4 beakers 2 in each group respectively. Shake each beaker 2 evenly, seal the mouth of each beaker 2 with a sealing film to prevent the pepsin reagent from splashing out, and shake each beaker 2 overnight for 12 hours to fully disperse the egg masses. The shaking temperature is set to 28°C and the speed is set to 200 rpm.

[0056] (3) pouring the mixed solution containing enzyme and egg particles in each beaker into a 200-mesh sieve for cleaning and filtration, washing the pepsin reagent remaining on the surface of the egg particles with tap water, and filtering to obtain 12 dispersed egg particles;

[0057] (4) The 12 dispersed eggs are placed one by one in 12 glass crystallization dishes, and the eggs in each glass crystallization dish are dried at 30°C. Then, the number of dried eggs in each glass crystallization dish is counted, and finally the number parameters of the eggs dispersed in each beaker are obtained.

[0058] Embodiment 4:

[0059] See also Figure 1-Figure 2 The dispersion effect of the Pomacea canaliculata eggs dispersed by shaking overnight for 12 hours in Example 1, Example 2, Example 3 and the comparative example was statistically analyzed to calculate the dispersion efficiency. The calculation formula is as follows:

[0060]

[0061] The dispersion efficiency results are shown in the following table:

[0062]

[0063] The experimental results show that the three hydrolases have a dispersion effect after shaking overnight for 12 hours compared with the control group without adding hydrolases. Among the three groups of hydrolases added with different mass concentrations, trypsin has the best dispersion effect, among which 0.25% trypsin has the best effect, with a dispersion rate of more than 98%.

[0064] The egg mass of Pomacea canaliculata before and after dispersion Figure 1 As shown by Figure 1 It can be seen that 0.25% trypsin can disperse the egg masses of Pomacea canaliculata evenly after 12 hours overnight, and the dispersed egg masses of Pomacea canaliculata can maintain the integrity of the egg particles, ensuring the smooth progress of the next experimental research.

[0065] The overnight shaking time in Example 1, Example 2, Example 3 and the comparative example was extended from 12 h to 48 h. It was found that except for the comparative example, the egg masses of the Pomacea canaliculata snails after adding different concentrations of hydrolase in Example 1, Example 2 and Example 3 could be completely dispersed.

[0066] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A method for dispersing Pomacea canaliculata egg masses using enzymes, characterized in that: The steps include: S1. Weigh a plurality of hydrolase powders of different weights and place them one by one in a plurality of beakers, and add an equal amount of distilled water to each of the plurality of beakers, stir until the hydrolase powder in each beaker is completely dissolved, and then use a microporous filter membrane to filter and sterilize the dissolved hydrolase solution in each beaker to obtain a plurality of sterile hydrolase solutions, and then put the plurality of sterile hydrolase solutions into sterile bottles one by one to obtain a plurality of hydrolase reagents of different concentrations, and store them in a low-temperature refrigerator for later use; S2, take a plurality of beakers 2, weigh a plurality of egg masses of equal weight and place them in a plurality of beakers 2 one by one, and add an equal amount of the hydrolase reagents of different concentrations obtained in S1 to a plurality of beakers 2 one by one, shake each beaker 2 evenly, seal each beaker 2 with a sealing film to prevent the solution from splashing out, and shake each beaker 2 overnight; S3, the mixed solution containing enzyme and egg grains in each beaker 2 is poured into the sieve respectively in turn for cleaning and filtering, the enzyme reagent remaining on the surface of the egg grains is rinsed with a cleaning agent, and multiple dispersed egg grains are obtained by filtering; S4. Place the multiple dispersed egg particles in multiple glass crystallization dishes one by one, and dry the egg particles in each glass crystallization dish. Then, count the number of the dried egg particles in each glass crystallization dish, and finally obtain the number parameters of the egg particles dispersed in each beaker.

2. A method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The hydrolase powder in S1 is any one of trypsin, pectinase and pepsin.

3. A method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The mass ratio of the hydrolase powder to the distilled water in the S1 is 0.1-1:100; the concentration of the hydrolase reagent in the S1 is 0.1-1%.

4. A method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The pore size range of the microporous filter membrane in the S1 is 0.22 to 1 μm; the low-temperature storage temperature of the hydrolase reagent in the S1 is set to -20°C to 4°C.

5. The method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The mass ratio of the egg mass to the hydrolase reagent in S2 is 0.1-1:1-20.

6. The method for dispersing Pomacea canaliculata egg masses using enzymes according to claim 1, characterized in that: In the S2, the shaking temperature is set to 25°C to 30°C; the shaking overnight time is set to 6 to 18 hours; and the shaking machine speed is set to 100 to 500 rpm.

7. The method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The mesh size of the sieve in S3 is set to 140-200 meshes.

8. The method for dispersing Pomacea canaliculata eggs using enzymes according to claim 1, characterized in that: The cleaning agent in S3 is any one of tap water, distilled water or deionized water.

9. The method for dispersing Pomacea canaliculata egg masses using enzymes according to claim 1, characterized in that: In S4, the drying temperature is set to 20-30°C.

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