Feed rich in prebiotics and probiotic compound and preparation process thereof

Abstract

The invention relates to a feed rich in prebiotics and probiotic compounds and a preparation process of the feed, and belongs to the technical field of feeds. The feed comprises a nutrition additive and a basal feed, the mass ratio of the nutrition additive to the basal feed is (0.3-0.4): 100, and the nutrition additive is prepared by mixing probiotics and a prebiotic additive according to the mass ratio of (2-3): (30-35); the probiotics consist of bacillus licheniformis freeze-dried powder, bacillus subtilis freeze-dried powder, lactobacillus acidophilus freeze-dried powder and saccharomyces cerevisiae freeze-dried powder. The bacillus subtilis and the saccharomyces cerevisiae are compounded to ferment the soybean meal, so that the content of polypeptides and other prebiotics in a fermentation product is effectively increased, and the nutritional value of the nutritional additive is further remarkably increased; the montmorillonite is used as a carrier, a soybean meal fermentation product can be attached to the montmorillonite, and then a natural high-stability material is used for modifying the montmorillonite, so that the stability of the nutrition additive and the ABTS + free radical scavenging capacity can be remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of feeds, and relates to a feed rich in a prebiotic and probiotic complex and a preparation process thereof. Background Art

[0002] In modern animal husbandry, in order to improve the health level and production performance of animals, the scientific ratio of feed is particularly important. Among them, prebiotics and probiotics, as feed additives, play a significant role in maintaining animal intestinal health, enhancing immunity and promoting the absorption of nutrients. Prebiotics such as oligoxylose, ganoderma lucidum polysaccharide or oligochitosan are added to feed, which can selectively promote the growth and activity of beneficial microorganisms in the intestine, while probiotics are living microorganisms that can produce health benefits to the host when added to feed in sufficient quantities. The addition of these two ingredients not only helps to improve the digestion and absorption function of animals, but also effectively prevents intestinal diseases and reduces the use of antibiotics, thereby improving breeding efficiency and product quality. The stability of prebiotics such as oligoxylose, ganoderma lucidum polysaccharide or oligochitosan in feed is affected by many factors, including the storage conditions of feed, the processing process of feed, and the interaction with other ingredients in feed. For example, oligoxylose is relatively stable under acidic conditions, but is easily degraded under alkaline conditions. Therefore, the pH control of feed is crucial to maintain the stability of oligoxylose. In addition, the high temperature and high pressure during feed processing may destroy the structure of prebiotics and affect their biological activity. Summary of the invention

[0003] The purpose of the present invention is to provide a feed rich in a prebiotic and probiotic complex and a preparation process thereof. The present invention ferments soybean meal with Bacillus subtilis and Saccharomyces cerevisiae to effectively increase the content of polypeptides and other prebiotics in the fermentation product, thereby significantly improving the nutritional value of the nutritional additive; using montmorillonite as a carrier, the soybean meal fermentation product can be attached to the montmorillonite, and then cysteine, cinnamaldehyde derivatives and eugenol form chemical grafting, which can significantly improve the stability of the nutritional additive and the ABTS+ free radical scavenging ability.

[0004] The purpose of the present invention can be achieved through the following technical solutions:

[0005] A feed rich in prebiotics and probiotics complexes comprises a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.3-0.4:100, and the nutritional additive is prepared by mixing probiotics and prebiotics additives in a mass ratio of 2-3:30-35.

[0006] As a preferred technical solution of the present invention, the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder in a mass ratio of 1-2:1-2:0.6-0.8:3-4.

[0007] As a preferred technical solution of the present invention, the live bacteria concentrations in the freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae are all 5×10 9 -2×10 12 Pieces / g.

[0008] As a preferred technical solution of the present invention, the preparation method of the prebiotic additive comprises the following steps:

[0009] Step 1: crushing, grinding, sieving, and sterilizing the soybean meal, and then adding deionized water and stirring to obtain a soybean meal base liquid;

[0010] Step 2, mixing the soybean meal base liquid and the mixed bacterial agent, fermenting, ultraviolet sterilizing, and drying to obtain a soybean meal fermentation product;

[0011] Step 3: After the soybean meal fermentation product, ethanol solution and montmorillonite are stirred and mixed, cysteine ​​and cinnamaldehyde derivatives are added, heated and stirred, eugenol and ammonium persulfate are added, and stirring is continued. The mixture is centrifuged and dried to obtain a prebiotic additive.

[0012] As a preferred technical solution of the present invention, in step one, the screening is through a 50-mesh sieve; the stirring and mixing is stirring at a speed of 300-500 r / min for 15-20 min.

[0013] As a preferred technical solution of the present invention, in step 1, the mass ratio of the soybean meal to deionized water is 38-40:60-65.

[0014] As a preferred technical solution of the present invention, in step 2, the fermentation is carried out at a temperature of 30-40°C for 42-50 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 30-35:20-22.

[0015] As a preferred technical solution of the present invention, in step 2, the concentration of Bacillus subtilis in the mixed bacterial agent is 10 7 -10 8 CFU / g, the concentration of Saccharomyces cerevisiae is 10 6 -10 7 CFU / g; the mixed bacterial agent further comprises the following components by weight: 6-8 parts of glucose, 0.2-0.4 parts of dipotassium hydrogen phosphate, 1.5-1.8 parts of potassium sorbate, 2.0-2.3 parts of fructose and 60-64 parts of deionized water;

[0016] As a preferred technical solution of the present invention, in step three, the stirring and mixing is stirring at a rotation speed of 500-600 rpm for 20-30 min; the heating and stirring is stirring at a temperature of 60-70°C for 6-7h; and the continued stirring is stirring at a temperature of 65-70°C for 4-5h.

[0017] As a preferred technical solution of the present invention, in step three, the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol and ammonium persulfate is 8-10:120-130:20-22:2.8-3.2:2.6-3.0:2.2-2.4:0.10-0.12; the concentration of the ethanol solution is 60wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

[0018] A method for preparing the feed rich in prebiotics and probiotics complex as mentioned above comprises the following steps: mixing the nutritional additive and the basic feed to obtain the feed.

[0019] Beneficial effects of the present invention:

[0020] The present invention ferments soybean meal with Bacillus subtilis and Saccharomyces cerevisiae to effectively increase the content of polypeptides and other prebiotics in the fermentation product, thereby significantly improving the nutritional value of the nutritional additive; using montmorillonite as a carrier, the soybean meal fermentation product can adhere to the montmorillonite, and then cysteine, cinnamaldehyde derivatives and eugenol form chemical grafting, which can significantly improve the stability of the nutritional additive and the ABTS+ free radical scavenging ability. DETAILED DESCRIPTION

[0021] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, characteristics and effects of the present invention are described in detail as follows in combination with the embodiments. The basic feed used in the following embodiments and comparative examples can be changed according to the feeding object; for example, for poultry, livestock and economic animals, the basic feed to be used is adjusted accordingly. Further examples of the basic feed composition used in the following embodiments and comparative examples are (wt%): 4.6% wheat bran, 23.8% soybean meal, 2.05% fish meal, 4.1% rapeseed meal, 1.35% calcium hydrogen phosphate, 0.27% salt, 0.04% vitamins (a mixture of equal proportions of vitamin B1, vitamin B2, vitamin B6, and vitamin C) and the remainder corn. The live bacteria concentration in Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder is about 5.5×10 11 Pieces / g.

[0022] Example 1

[0023] A feed rich in a prebiotic and probiotic complex comprises a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.3:100, the nutritional additive is formed by mixing probiotics and prebiotic additives in a mass ratio of 2:30; the probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae in a mass ratio of 1:1:0.6:3.

[0024] The preparation method of the prebiotic additive comprises the following steps:

[0025] Step 1: crush, grind, sieve and sterilize the soybean meal, add deionized water and stir to obtain soybean meal base liquid; wherein the sieving is through a 50-mesh sieve; the stirring is stirring at a speed of 300 r / min for 15 minutes. The mass ratio of the soybean meal to the deionized water is 38:60.

[0026] Step 2: Mix the soybean meal base liquid and the mixed bacterial agent, ferment, sterilize with ultraviolet light, and dry to obtain soybean meal fermentation product; wherein the fermentation is performed at 32°C for 42 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 30:20; the concentration of Bacillus subtilis in the mixed bacterial agent is 10 7 CFU / g, the concentration of Saccharomyces cerevisiae is 10 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 6 parts of glucose, 0.2 parts of dipotassium hydrogen phosphate, 1.5 parts of potassium sorbate, 2.0 parts of fructose and 60 parts of deionized water;

[0027] Step 3: After stirring and mixing the soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine ​​and cinnamaldehyde derivatives, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, and dry to obtain a prebiotic additive; wherein the stirring and mixing is stirring at a speed of 500 rpm for 20 minutes; the heating and stirring is stirring at a temperature of 60°C for 6 hours; the continued stirring is stirring at a temperature of 65°C for 4 hours; the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivatives, eugenol and ammonium persulfate is 8:120:20:2.8:2.6:2.2:0.10; the concentration of the ethanol solution is 60wt%; the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

[0028] A method for preparing a feed rich in prebiotics and probiotics complex comprises the following steps: mixing a nutrient additive and a basic feed to obtain the feed.

[0029] Example 2

[0030] A feed rich in a complex of prebiotics and probiotics comprises a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.33:100, the nutritional additive is formed by mixing probiotics and prebiotic additives in a mass ratio of 2.3:32; the probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae in a mass ratio of 1.3:1.3:0.67:3.3.

[0031] The preparation method of the prebiotic additive comprises the following steps:

[0032] Step 1: crush, grind, sieve and sterilize the soybean meal, add deionized water and stir to obtain soybean meal base liquid; wherein the sieving is through a 50-mesh sieve; the stirring and mixing is stirring at a speed of 360 r / min for 17 minutes. The mass ratio of the soybean meal to the deionized water is 38.7:62.

[0033] Step 2: Mix the soybean meal base liquid and the mixed bacterial agent, ferment, sterilize with ultraviolet light, and dry to obtain soybean meal fermentation product; wherein the fermentation is performed at 32°C for 45 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 32:20.7; the concentration of Bacillus subtilis in the mixed bacterial agent is 5*10 7 CFU / g, the concentration of Saccharomyces cerevisiae is 5*10 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 6.7 parts of glucose, 0.27 parts of dipotassium hydrogen phosphate, 1.6 parts of potassium sorbate, 2.1 parts of fructose and 61 parts of deionized water;

[0034] Step 3: After stirring and mixing the soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine ​​and cinnamaldehyde derivatives, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, and dry to obtain a prebiotic additive; wherein the stirring and mixing is stirring at a speed of 530 rpm for 25 minutes; the heating and stirring is stirring at a temperature of 63°C for 6.5 hours; the continued stirring is stirring at a temperature of 67°C for 4.5 hours; the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivatives, eugenol and ammonium persulfate is 8.7:123:20.7:2.9:2.7:2.27:0.115; the concentration of the ethanol solution is 60wt%; the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

[0035] A method for preparing a feed rich in prebiotics and probiotics complex comprises the following steps: mixing a nutrient additive and a basic feed to obtain the feed.

[0036] Example 3

[0037] A feed rich in a prebiotic and probiotic complex comprises a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.37:100, the nutritional additive is formed by mixing probiotics and prebiotic additives in a mass ratio of 2.7:33; the probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae in a mass ratio of 1.7:1.7:0.73:3.7.

[0038] The preparation method of the prebiotic additive comprises the following steps:

[0039] Step 1: crush, grind, sieve and sterilize the soybean meal, add deionized water and stir to obtain soybean meal base liquid; wherein the sieving is through a 50-mesh sieve; the stirring and mixing is stirring at a speed of 430 r / min for 18 minutes. The mass ratio of the soybean meal to the deionized water is 39.3:63.

[0040] Step 2: Mix the soybean meal base liquid and the mixed bacterial agent, ferment, sterilize with ultraviolet light, and dry to obtain soybean meal fermentation product; wherein the fermentation is performed at 32°C for 47 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 33:21.3; the concentration of Bacillus subtilis in the mixed bacterial agent is 5*10 7 CFU / g, the concentration of Saccharomyces cerevisiae is 5*10 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 7.3 parts of glucose, 0.33 parts of dipotassium hydrogen phosphate, 1.7 parts of potassium sorbate, 2.2 parts of fructose and 63 parts of deionized water;

[0041] Step 3: After stirring and mixing the soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine ​​and cinnamaldehyde derivatives, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, and dry to obtain a prebiotic additive; wherein the stirring and mixing is stirring at a speed of 560 rpm for 25 minutes; the heating and stirring is stirring at a temperature of 67°C for 6.5 hours; the continued stirring is stirring at a temperature of 68°C for 4.5 hours; the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivatives, eugenol and ammonium persulfate is 9.3:127:21.3:3.1:2.9:2.33:0.115; the concentration of the ethanol solution is 60wt%; the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

[0042] A method for preparing a feed rich in prebiotics and probiotics complex comprises the following steps: mixing a nutrient additive and a basic feed to obtain the feed.

[0043] Example 4

[0044] A feed rich in a prebiotic and probiotic complex comprises a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.4:100, the nutritional additive is formed by mixing probiotics and prebiotic additives in a mass ratio of 3:35; the probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae in a mass ratio of 2:2:0.8:4.

[0045] The preparation method of the prebiotic additive comprises the following steps:

[0046] Step 1: crush, grind, sieve and sterilize the soybean meal, add deionized water and stir to obtain soybean meal base liquid; wherein the sieving is through a 50-mesh sieve; the stirring and mixing is stirring at a speed of 500 r / min for 20 minutes. The mass ratio of the soybean meal to the deionized water is 40:65.

[0047] Step 2: Mix the soybean meal base liquid and the mixed bacterial agent, ferment, sterilize with ultraviolet light, and dry to obtain soybean meal fermentation product; wherein the fermentation is performed at 32°C for 50 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 35:22; the concentration of Bacillus subtilis in the mixed bacterial agent is 10 8 CFU / g, the concentration of Saccharomyces cerevisiae is 10 7 CFU / g; the mixed bacterial agent also includes the following components by weight: 8 parts of glucose, 0.4 parts of dipotassium hydrogen phosphate, 1.8 parts of potassium sorbate, 2.3 parts of fructose and 64 parts of deionized water;

[0048] Step 3: After stirring and mixing the soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine ​​and cinnamaldehyde derivatives, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, and dry to obtain a prebiotic additive; wherein the stirring and mixing is stirring at a speed of 600 rpm for 30 minutes; the heating and stirring is stirring at a temperature of 70°C for 7 hours; the continued stirring is stirring at a temperature of 70°C for 5 hours; the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivatives, eugenol and ammonium persulfate is 10:130:22:3.2:3.0:2.4:0.12; the concentration of the ethanol solution is 60wt%; the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

[0049] A method for preparing a feed rich in prebiotics and probiotics complex comprises the following steps: mixing a nutrient additive and a basic feed to obtain the feed.

[0050] Comparative Example 1

[0051] Compared with Example 3, Comparative Example 1 is different in that montmorillonite is not used, and the other components, preparation steps and parameters are the same.

[0052] Comparative Example 2

[0053] Compared with Example 3, Comparative Example 2 is different in that cysteine ​​is not used, and the other components, preparation steps and parameters are the same.

[0054] Comparative Example 3

[0055] Compared with Example 3, Comparative Example 3 is different in that cinnamaldehyde is used instead of the cinnamaldehyde derivative, and the other components, preparation steps and parameters are the same.

[0056] Comparative Example 4

[0057] Compared with Example 3, Comparative Example 4 is different in that no cinnamaldehyde derivative is used, and the remaining components, preparation steps and parameters are the same.

[0058] Comparative Example 5

[0059] Compared with Example 3, Comparative Example 5 is different in that eugenol is not used, and the other components, preparation steps and parameters are the same.

[0060] The prebiotic additives prepared in Examples 1-4 and Comparative Examples 1-5 were subjected to the following performance tests, respectively. The test results are shown in Table 1.

[0061] ABTS + Free radical scavenging rate = (A 空白 -A 样品 ) / A 空白 *100%

[0062] Table 1: Test results

[0063] ABTS+ free radical scavenging rate (%) ABTS+ free radical scavenging rate after 24h treatment at 50℃ (%) Example 1 92.8 91.2 Example 2 93.3 91.6 Example 3 93.6 92.1 Example 4 93.4 91.6 Comparative Example 1 75.7 69.4 Comparative Example 2 78.6 73.2 Comparative Example 3 84.7 79.8 Comparative Example 4 77.8 71.6 Comparative Example 5 86.2 81.3

[0064] From the test results in Table 1, it can be seen that compared with Comparative Examples 1-5, the prebiotic additives prepared in Examples 1-4 of the present invention have excellent stability and ABTS+ free radical scavenging ability.

[0065] Through comparative analysis, it can be known that this is because the present invention uses Bacillus subtilis and Saccharomyces cerevisiae to ferment soybean meal, effectively increasing the content of polypeptides and other prebiotics in the fermentation product, thereby significantly improving the nutritional value of the nutritional additive; using montmorillonite as a carrier, the soybean meal fermentation product can adhere to the montmorillonite to increase its own stability, the thiol group of cysteine ​​can form a coordination bond adsorption with the metal ion of montmorillonite, and promote the attachment of cysteine, and then cysteine ​​forms a chemical graft with the cinnamaldehyde derivative and forms a coating on the soybean meal fermentation product and montmorillonite, introducing a Schiff base structure with strong antioxidant ability and high stability, and the oxygen-containing functional group on the cinnamaldehyde derivative has higher antioxidant ability than cinnamaldehyde, combined with its own highly stable benzene ring structure, it can significantly improve the stability and antioxidant ability of the prebiotic additive, and the double bond carried by the cinnamaldehyde derivative can be grafted with eugenol, further improving the stability of the prebiotic additive and the ABTS+ free radical scavenging ability.

[0066] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any indirect modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A feed rich in a prebiotic and probiotic complex, comprising a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.3-0.4:100, characterized in that: The nutritional additive is prepared by mixing probiotics and prebiotic additives in a mass ratio of 2-3:30-35; the probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae.

2. A feed rich in a prebiotic and probiotic complex according to claim 1, characterized in that: The probiotics are composed of freeze-dried powder of Bacillus licheniformis, freeze-dried powder of Bacillus subtilis, freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Saccharomyces cerevisiae in a mass ratio of 1-2:1-2:0.6-0.8:3-4.

3. A feed rich in a prebiotic and probiotic complex according to claim 1, characterized in that: The preparation method of the prebiotic additive comprises the following steps: Step 1: crushing, grinding, sieving, and sterilizing the soybean meal, and then adding deionized water and stirring to obtain a soybean meal base liquid; Step 2, mixing the soybean meal base liquid and the mixed bacterial agent, fermenting, ultraviolet sterilizing, and drying to obtain a soybean meal fermentation product; Step 3: After the soybean meal fermentation product, ethanol solution and montmorillonite are stirred and mixed, cysteine ​​and cinnamaldehyde derivatives are added, heated and stirred, eugenol and ammonium persulfate are added, and stirring is continued. The mixture is centrifuged and dried to obtain a prebiotic additive.

4. A feed rich in a prebiotic and probiotic complex according to claim 3, characterized in that: In step 1, the sieving is through a 50-mesh sieve; and the stirring and mixing is stirring at a speed of 300-500 r / min for 15-20 min.

5. The feed rich in prebiotics and probiotics complex according to claim 3, characterized in that: In step 1, the mass ratio of the soybean meal to deionized water is 38-40:60-65.

6. The feed rich in prebiotics and probiotics complex according to claim 3, characterized in that: In step 2, the fermentation is carried out at a temperature of 30-40° C. for 42-50 hours; the mass ratio of the soybean meal base liquid to the mixed bacterial agent is 30-35:20-22.

7. The feed rich in prebiotics and probiotics complex according to claim 3, characterized in that: In step 2, the concentration of Bacillus subtilis in the mixed bacterial agent is 10 7 -10 8 CFU / g, the concentration of Saccharomyces cerevisiae is 10 6 -10 7 CFU / g; the mixed bacterial agent also includes the following components in weight ratio: 6-8 parts of glucose, 0.2-0.4 parts of dipotassium hydrogen phosphate, 1.5-1.8 parts of potassium sorbate, 2.0-2.3 parts of fructose and 60-64 parts of deionized water.

8. The feed rich in prebiotics and probiotics complex according to claim 3, characterized in that: In step three, the stirring and mixing is stirring at a rotation speed of 500-600 rpm for 20-30 minutes; the heating and stirring is stirring at a temperature of 60-70°C for 6-7 hours; and the continued stirring is stirring at a temperature of 65-70°C for 4-5 hours.

9. The feed rich in prebiotics and probiotics complex according to claim 3, characterized in that: In step three, the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol and ammonium persulfate is 8-10:120-130:20-22:2.8-3.2:2.6-3.0:2.2-2.4:0.10-0.12; the concentration of the ethanol solution is 60wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.

10. A method for preparing a feed rich in a prebiotic and probiotic complex according to any one of claims 1 to 9, characterized in that: The following steps are involved: The nutritional additives and basic feed are mixed together to obtain the product.