Mushroom probiotic chewable tablet and preparation method thereof

Abstract

The invention discloses a shiitake mushroom probiotic chewable tablet and a preparation method, and belongs to the field of chewable tablet processing, and the method comprises the following steps: uniformly mixing cured shiitake mushroom powder, microcrystalline cellulose, mannitol and citric acid according to a mass ratio of (4-4.2): (1.7-1.9): (2-2.2): 0.1 to obtain a mixture, and then sequentially granulating and drying the mixture to obtain particles; the probiotics are activated and then subjected to vacuum freeze drying under the protection of a composite freeze-drying protective agent, the composite freeze-drying protective agent is skimmed milk powder, cured shiitake mushroom powder and trehalose, then screening is conducted, and freeze-dried probiotic powder is obtained; the mass ratio of the freeze-dried probiotic powder to the particles is (1.5-2): (8-8.5), and the freeze-dried probiotic powder and the particles are mixed and tableted to obtain the shiitake mushroom probiotic chewable tablets, so that the physiological functions of the shiitake mushrooms and the probiotics are effectively combined, and the health-care effects of enhancing immunity and preventing cancers are synergistically exerted.

Description

Technical Field

[0001] The invention belongs to the field of chewable tablet processing, and particularly relates to a shiitake probiotic chewable tablet and a preparation method thereof. Background Art

[0002] Shiitake mushroom is a kind of fungus that can be used as both medicine and food. It is rich in protein, vitamins, minerals and various biologically active ingredients. The polysaccharides, organic bases, purines and other substances in shiitake mushrooms have the functions of anti-tumor, lowering blood lipids and enhancing immunity. Shiitake mushrooms also contain ergosterol, which is a precursor for the synthesis of vitamin D. Therefore, shiitake mushrooms can promote calcium absorption and maintain normal bone development. Probiotics refer to a class of living microorganisms that have a beneficial effect on the health of the host. They have the functions of maintaining the balance of intestinal flora, enhancing immunity, and preventing cancer. Therefore, if the physiological functions of shiitake mushrooms and probiotics can be combined and work synergistically, it will have a good health care effect.

[0003] However, at this stage, although there are many kinds of foods containing probiotics, in addition to fermented products, there are also a variety of probiotic powders, tablets and added products, but there are no reports of foods containing both shiitake mushrooms and probiotics. Related to this is the Chinese patent entitled "A Multifunctional Probiotic Composition and Its Application" with application number 202210785020.9. The probiotic composition includes probiotic powder, prebiotic powder and Chinese herbal materials. The Chinese herbal materials include Cordyceps militaris and shiitake mushrooms or their extracts and turmeric. It reduces the number of probiotic species and uses a variety of Chinese herbal materials to assist in enhancing the effects of probiotics, reducing costs while ensuring efficacy. However, it still cannot combine the physiological functions of shiitake mushrooms and probiotics to synergistically play a good health care role. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention provides a shiitake mushroom probiotic chewable tablet and a preparation method thereof, so as to solve the problem of effectively combining the physiological functions of shiitake mushrooms and probiotics to synergistically play the health care effects of enhancing immunity and preventing cancer, and uses shiitake mushroom powder as a protective agent to improve the freeze-dried survival rate of probiotics. At the same time, the tablet is convenient to eat and easy to carry.

[0005] The present invention is achieved through the following technical solutions: A method for preparing shiitake mushroom probiotic chewable tablets comprises the following steps: S1, mixing the cooked mushroom powder, microcrystalline cellulose, mannitol and citric acid in a mass ratio of (4-4.2):(1.7-1.9):(2-2.2):0.1 to obtain a mixture, and then granulating and drying the mixture in sequence to obtain granules; The activated probiotics are vacuum freeze-dried under the protection of a composite freeze-drying protective agent, wherein the composite freeze-drying protective agent is skim milk powder, matured mushroom powder and trehalose, and then sieved to obtain freeze-dried probiotic powder; S2, with a mass ratio of (1.5-2): (8-8.5), the freeze-dried probiotic powder and the granules are mixed and tableted to obtain shiitake mushroom probiotic chewable tablets.

[0006] Preferably, the matured mushroom powder in S1 is obtained by the following process: Wash the mushrooms, slice them, and then ripen them. Finally, dry, crush and sieve them in sequence to obtain ripened mushroom powder.

[0007] Furthermore, the washed mushrooms are cut into slices with a thickness of 2 to 4 mm, steamed for 10 to 20 minutes, and finally dried, crushed and sieved in sequence.

[0008] Furthermore, the steamed mushrooms are dried until the moisture content accounts for less than 10% of the total mass, and then crushed, and then passed through a 150-250 mesh sieve to obtain mature mushroom powder.

[0009] Preferably, in S1, the activated probiotics are mixed evenly with skim milk powder, cooked mushroom powder and trehalose to obtain a mixture, and then the mixture is vacuum freeze-dried, and the masses of skim milk powder, cooked mushroom powder and trehalose in each 1 mL of the mixture are 0.1 g, 0.05 g and 0.25 g, respectively.

[0010] Furthermore, the vacuum freeze-drying is carried out at 20° C. for 24-28 hours.

[0011] Preferably, S1 activates the probiotics and freeze-dries them in a vacuum under the protection of a composite freeze-drying protective agent, and then passes them through a 100-mesh sieve to obtain freeze-dried probiotic powder.

[0012] Preferably, S1 adds ethanol to the mixture and mixes evenly, wherein the mass of ethanol accounts for 70% of the mixture, to obtain a soft material, and then the soft material is put into a granulator for granulation.

[0013] Further, S1 granulates the mixture, passes it through a 20-mesh sieve for granulation, and then dries it to obtain granules.

[0014] A shiitake mushroom probiotic chewable tablet obtained by the preparation method of shiitake mushroom probiotic chewable tablets described in any one of the above.

[0015] Compared with the prior art, the present invention has the following beneficial technical effects: The present invention uses the ripened shiitake powder and freeze-dried probiotic powder as main raw materials, adds mannitol and citric acid as flavor correctants, first activates the probiotics and then freeze-dries them under the protection of skim milk powder, ripened shiitake powder and trehalose, can reduce the damage of low temperature to the probiotics, keep the probiotic powder with a high number of viable bacteria, improve the freeze-dried survival rate of the probiotics, and granulate the ripened shiitake powder, microcrystalline cellulose, mannitol and citric acid after mixing, microcrystalline cellulose can realize the uniformity of particles as a filler, and then presses the processed probiotics into shiitake probiotic chewable tablets, which have both edible fungi and probiotic physiological activities, can not only maintain the balance of intestinal flora, enhance immunity, and prevent cancer, but also can resist tumors, reduce blood lipids, promote calcium absorption, maintain normal bone development, and have the characteristics of being healthy, delicious and easy to carry. The present invention has simple process, is suitable for factory production, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a process flow chart of the present invention.

[0017] Figure 2 This is a physical picture of the shiitake mushroom probiotic chewable tablets obtained in Example 1 of the present invention.

[0018] Figure 3 This is a graph showing the effect of the single freeze-drying protective agent trehalose content on the survival rate of probiotics described in the present invention.

[0019] Figure 4 This is a graph showing the effect of the content of skim milk powder, a single freeze-drying protective agent, on the survival rate of probiotics.

[0020] Figure 5 This is a graph showing the effect of the content of the single freeze-dried protective agent shiitake mushroom powder on the survival rate of probiotics described in the present invention.

[0021] Figure 6 This is a graph showing the effect of different combinations of single lyophilized protective agents described in the present invention on the survival rate of probiotics. DETAILED DESCRIPTION

[0022] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, which is intended to explain the present invention rather than to limit it.

[0023] A method for preparing a shiitake mushroom probiotic chewable tablet, Figure 1 As shown, the following steps are included: Step 1, wash the fresh mushrooms, cut them into slices with a thickness of 2 to 4 mm, steam them for 10 to 20 minutes, take them out, and then dry them at 45 to 55° C. for 36 to 48 hours until the moisture content is less than 10% of the total mass, then crush them, pass them through a 150 to 250 mesh sieve, and set them aside.

[0024] Step 2, adding a freeze-drying protective agent after the probiotics are activated. The probiotics can be freely selected and combined from species such as Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus paracasei. The probiotics are vacuum freeze-dried at -20°C for 24-28 hours, and passed through a 100-mesh sieve to obtain freeze-dried probiotic powder for later use.

[0025] The freeze-drying protective agents are skim milk powder, the mushroom powder in step 1, and trehalose, accounting for 10%, 5% and 25% (w / v) of the total volume, respectively.

[0026] Step 3, according to the mass ratio, respectively take the mushroom powder, microcrystalline cellulose, mannitol and citric acid in step 1 and put them into a mixer to mix evenly, the mass ratio of mushroom powder: microcrystalline cellulose: mannitol: citric acid is (4-4.2): (1.7-1.9): (2-2.2): 0.1, add ethanol accounting for 70% of the mass of the mixture to the mixture and mix evenly to make a soft material, and then put it into a granulator for granulation, then pass through a 20-mesh sieve for whole grains, and dry in an oven, finally put it into a tablet press with freeze-dried probiotic powder, mix and tablet, the mass ratio of freeze-dried probiotic powder to granules is (1.5-2): (8-8.5), and the mushroom probiotic chewable tablets are obtained after packaging.

[0027] Embodiment 1: A method for preparing shiitake mushroom probiotic chewable tablets, the preparation process of which comprises the following steps: Preparation of mushroom powder: After being selected and rinsed, the mushrooms were cut into 3 mm slices and evenly spread in a steamer for 15 min. Then the samples were taken out and spread flat in an electric blast drying oven with the temperature set to 55 °C and the drying time set to 36 h.

[0028] Take out the dried sample and put it in a grinder for 5 minutes, then take it out and pass it through a 200-mesh sieve. The unsieved sample is crushed again. Repeat the operation three times to obtain mushroom powder.

[0029] Prepare probiotic powder: After activation, the probiotics were centrifuged to obtain bacterial sludge, which was mixed with a freeze-drying protective agent to obtain a mixture. The freeze-drying protective agent contained 10% skim milk powder, 5% mushroom powder, and 25% trehalose, that is, the masses of skim milk powder, mushroom powder and trehalose in each 1 mL of the mixture were 0.1 g, 0.05 g and 0.25 g, respectively. The mixture was vacuum freeze-dried at -20°C for 24 h and passed through a 100-mesh sieve to obtain freeze-dried probiotic powder.

[0030] Preparation of Shiitake Mushroom Probiotic Chewable Tablets: Mixing of main and auxiliary materials: according to the mass ratio, put 40 parts of mushroom powder, 20 parts of mannitol, 19 parts of microcrystalline cellulose and 1 part of citric acid into a mixer and mix evenly.

[0031] Tableting: Add 70% ethanol to the mixed material to make a soft material, then put it into a granulator for granulation, pass it through a 20-mesh sieve for whole particles, and dry it until the moisture content is less than 5%. Finally, mix the freeze-dried probiotic powder with the dried particles in a mass fraction ratio of 2:8, put it into a tablet press for tableting, and package it to obtain the finished product.

[0032] like Figure 2 It can be seen that the color of the shiitake mushroom probiotic chewable tablets obtained in this example is darker than that of shiitake mushrooms, and the particles are fuller.

[0033] In order to verify that the composite lyophilization protectant of this example can maximize the lyophilization survival rate of probiotics, a comparative experiment was conducted.

[0034] Solution 1: After activation, the probiotics were centrifuged to obtain bacterial sludge, which was mixed with different contents (0%, 15%, 20%, 25% and 30%) of freeze-drying protective agent trehalose to obtain a mixture, that is, 0g, 0.15g, 0.2g, 0.25g, and 0.3g of trehalose per 1mL of the mixture. The mixture was vacuum freeze-dried at -20℃ for 24h and passed through a 100-mesh sieve to obtain probiotic powder. The measured results are as follows Figure 3 As shown, it can be seen that the probiotic survival rate is highest at 25% content.

[0035] Option 2: After activation, the probiotics were centrifuged to obtain bacterial sludge, which was then mixed with skim milk powder with different contents (0%, 5%, 10%, 15% and 20%) of freeze-drying protective agent to obtain a mixture, that is, 0g, 0.05g, 0.1g, 0.15g, and 0.2g of skim milk powder per 1mL of the mixture. The mixture was vacuum freeze-dried at -20℃ for 24h and passed through a 100-mesh sieve to obtain probiotic powder. The measured results are as follows Figure 4 As shown, it can be seen that the probiotic survival rate is highest at 10% content.

[0036] Option 3: After activation, the probiotics were centrifuged to obtain bacterial sludge, which was then mixed with freeze-dried mushroom powder of different contents (0%, 2.5%, 5%, 7.5% and 10%) to obtain a mixture, i.e., 0g, 0.025g, 0.05g, 0.75g and 0.1g of mushroom powder per 1mL of the mixture. The mixture was freeze-dried at -20℃ for 24h and passed through a 100-mesh sieve to obtain the probiotic powder. The measured results are as follows Figure 5 As shown, it can be seen that the probiotic survival rate is highest at 25% content.

[0037] The skim milk powder, mushroom powder and trehalose content with the highest survival rate were selected, and different types of combinations were performed to obtain the following results: Figure 6From the results shown, it can be found that the survival rate of probiotics is the highest when skim milk powder, mushroom powder and trehalose exist at the same time, so 10% skim milk powder, 5% mushroom powder and 25% trehalose are selected.

[0038] Embodiment 2: A method for preparing shiitake mushroom probiotic chewable tablets, the preparation process of which comprises the following steps: Preparation of mushroom powder: After being selected and rinsed, the mushrooms were cut into 3 mm slices and evenly spread in a steamer for 15 min. Then the samples were taken out and spread flat in an electric blast drying oven with the temperature set to 55 °C and the drying time set to 36 h.

[0039] Take out the dried sample and put it in a grinder for 5 minutes, then take it out and pass it through a 200-mesh sieve. The unsieved sample is crushed again. Repeat the operation three times to obtain mushroom powder.

[0040] Prepare probiotic powder: After activation, the probiotics were centrifuged to obtain bacterial mud, which was mixed with a freeze-drying protective agent containing 10% skim milk powder, 5% mushroom powder, and 25% trehalose. The mixture was vacuum freeze-dried at -20°C for 24 hours and passed through a 100-mesh sieve to obtain freeze-dried probiotic powder.

[0041] Preparation of Shiitake Mushroom Probiotic Chewable Tablets: Mixing of main and auxiliary materials: according to the mass ratio, put 42 parts of mushroom powder, 20 parts of mannitol, 19 parts of microcrystalline cellulose and 1 part of citric acid into a mixer and mix evenly.

[0042] Tableting: Add 70% ethanol to the mixed material to make a soft material, then put it into a granulator for granulation, pass it through a 20-mesh sieve for whole particles, and dry it until the moisture content is less than 5%. Finally, mix the freeze-dried probiotic powder with the dried particles in a mass fraction ratio of 1.8:8.2, put it into a tablet press for tableting, and package it to obtain the finished product.

[0043] Embodiment 3: A method for preparing shiitake mushroom probiotic chewable tablets, the preparation process of which comprises the following steps: Preparation of mushroom powder: After being selected and rinsed, the mushrooms were cut into 3 mm slices and evenly spread in a steamer for 15 min. Then the samples were taken out and spread flat in an electric blast drying oven with the temperature set to 55 °C and the drying time set to 36 h.

[0044] Take out the dried sample and put it in a grinder for 5 minutes, then take it out and pass it through a 200-mesh sieve. The unsieved sample is crushed again. Repeat the operation three times to obtain mushroom powder.

[0045] Prepare probiotic powder: After activation, the probiotics were centrifuged to obtain bacterial mud, which was mixed with a freeze-drying protective agent containing 10% skim milk powder, 5% shiitake mushroom powder, and 25% trehalose. The mixture was vacuum freeze-dried for 24 hours and passed through a 100-mesh sieve to obtain freeze-dried probiotic powder.

[0046] Preparation of Shiitake Mushroom Probiotic Chewable Tablets: Mixing of main and auxiliary materials: according to the mass ratio, put 40 parts of mushroom powder, 22 parts of mannitol, 17 parts of microcrystalline cellulose and 1 part of citric acid into a mixer and mix evenly.

[0047] Tableting: Add 70% ethanol to the mixed material to make a soft material, then put it into a granulator for granulation, pass it through a 20-mesh sieve for whole particles, and dry it until the moisture content is less than 5%. Finally, mix the freeze-dried probiotic powder with the dried particles in a mass fraction ratio of 1.5:8.5, put it into a tablet press for tableting, and package it to obtain the finished product.

Claims

1. A method for preparing a shiitake mushroom probiotic chewable tablet, characterized in that: The following steps are involved: S1, mixing the cooked mushroom powder, microcrystalline cellulose, mannitol and citric acid in a mass ratio of (4-4.2):(1.7-1.9):(2-2.2):0.1 to obtain a mixture, and then granulating and drying the mixture in sequence to obtain granules; The activated probiotics are vacuum freeze-dried under the protection of a composite freeze-drying protective agent, wherein the composite freeze-drying protective agent is skim milk powder, matured mushroom powder and trehalose, and then sieved to obtain freeze-dried probiotic powder; S2, with a mass ratio of (1.5-2): (8-8.5), the freeze-dried probiotic powder and the granules are mixed and tableted to obtain shiitake mushroom probiotic chewable tablets.

2. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 1, characterized in that: The cooked mushroom powder described in S1 is obtained by the following process: Wash the mushrooms, slice them, and then ripen them. Finally, dry, crush and sieve them in sequence to obtain ripened mushroom powder.

3. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 2, characterized in that: Cut the washed mushrooms into slices with a thickness of 2 to 4 mm, steam them for 10 to 20 minutes, and finally dry, crush and sieve them.

4. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 2, characterized in that: The steamed shiitake mushrooms are dried until the water content accounts for less than 10% of the total mass, and then crushed and passed through a 150-250 mesh sieve to obtain cooked shiitake mushroom powder.

5. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 1, characterized in that: In S1, the activated probiotics are mixed evenly with skim milk powder, cooked mushroom powder and trehalose to obtain a mixture, and then the mixture is vacuum freeze-dried. The masses of skim milk powder, cooked mushroom powder and trehalose in each 1 mL of the mixture are 0.1 g, 0.05 g and 0.25 g, respectively.

6. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 5, characterized in that: The vacuum freeze drying is carried out at 20°C for 24-28h.

7. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 1, characterized in that: S1: Activate the probiotics and freeze-dry them in vacuum under the protection of a composite freeze-drying protective agent, then pass them through a 100-mesh sieve to obtain freeze-dried probiotic powder.

8. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 1, characterized in that: S1 adds ethanol to the mixture and mixes evenly, wherein the mass of ethanol accounts for 70% of the mixture, to obtain a soft material, and then the soft material is put into a granulator for granulation.

9. The method for preparing the shiitake mushroom probiotic chewable tablets according to claim 8, characterized in that: S1: After the mixture is granulated, it is sieved through a 20-mesh sieve for size adjustment and then dried to obtain granules.

10. A shiitake mushroom probiotic chewable tablet obtained by the preparation method of the shiitake mushroom probiotic chewable tablet according to any one of claims 1 to 9.

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