Viola tianshanica extract facial cleanser and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a viola tianshanica extract facial cleanser and a preparation method thereof. The shampoo is prepared from the following components in percentage by weight: 2%-8% of effective active ingredients, 5%-15% of a foaming agent, 1%-7% of a solubilizer, 2%-11% of an emulsifier, 8%-12% of a humectant, 0.1%-2% of a preservative, 0.5%-5% of a penetrant and the balance of deionized water, the effective active component is composed of a viola tianshanensis extract, and the viola tianshanensis extract accounts for 1%-5% of the viola tianshanensis extract facial cleanser; the viola tianshanensis extract is extracted from a medicinal part of viola tianshanensis. The prepared viola tianshanica extract facial cleanser is mild, free of irritation and high in safety, has the anti-oxidation and anti-inflammatory characteristics, and can effectively resist damage of the external environment to skin, slow down aging of the skin and mildly regulate the skin.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a Viola tianshanica extract facial cleanser and a preparation method thereof. Background Art

[0002] With the rapid development of the economy, people's living habits have also changed. The intake of high-sugar and high-fat foods in daily life has increased significantly, resulting in vigorous oil secretion in the body, which provides conditions for the breeding of skin problems. At the same time, long-term exposure to computer screens and mobile phones will not only expose the skin to electromagnetic radiation for a long time, but also easily cause increased oil secretion of the skin and clogged pores, leading to skin problems such as acne and pimples. In addition, electromagnetic radiation will interfere with the normal metabolism of the skin, accelerate skin aging, make the skin dull and dry, and even cause pigmentation to form spots.

[0003] Facial cleansers are a type of facial cleansing product that has the functions of cleaning and controlling oil. Currently, there are many facial cleansing products on the market, most of which are chemically synthesized, such as soap-based facial cleansers. Long-term use may cause excessive degreasing of the skin, thereby damaging the skin barrier and being highly irritating. The main function of facial cleansing products in the prior art is to deeply cleanse the skin and remove dirt, oil, dead skin cells and makeup residues on the skin surface. However, there are still limitations in dealing with skin inflammation, sensitivity problems and promoting skin improvement, and it is difficult to meet consumers' needs for comprehensive and targeted skin care.

[0004] Therefore, it is urgent to develop a facial cleanser that has both anti-inflammatory and anti-allergic effects. Summary of the invention

[0005] In order to develop a biological cleansing product with high safety and both anti-inflammatory and anti-allergic properties, the present invention provides a facial cleanser containing Viola tianshanica extract and a preparation method thereof. The facial cleanser containing Viola tianshanica extract provided by the present invention is not only mild and non-irritating, but also highly safe and has anti-allergic, antioxidant and anti-inflammatory properties, and can effectively resist the damage of the external environment to the skin, slow down the aging of the skin, gently regulate the skin quality, and reduce the formation of acne.

[0006] A facial cleanser containing Viola tianshanica extract is prepared from the following components in percentage by weight: 2% to 8% of effective active ingredients, 5% to 15% of foaming agent, 1% to 7% of solubilizer, 2% to 11% of emulsifier, 8% to 12% of moisturizer, 0.1% to 2% of preservative, 0.5% to 5% of penetrant, and the balance is deionized water.

[0007] The effective active ingredient is composed of Viola tianshanica extract, and the Viola tianshanica extract accounts for 1% to 5% of the facial cleanser.

[0008] The Viola tianshanica extract is obtained by extracting the medicinal part of Viola tianshanica through ethanol.

[0009] The invention uses the extract of Viola tianshanica as a raw material, and is prepared by combining with a foaming agent, a solubilizing agent, an emulsifier, a moisturizing agent, a preservative and a penetrant. The Viola tianshanica facial cleanser prepared by the invention has stable foaming, good cleaning effect, safe ingredients, mild and non-irritating, and is suitable for a variety of skin types; it also has anti-allergic, anti-oxidant and anti-inflammatory properties, can effectively resist the damage of the external environment to the skin, slow down the aging of the skin, gently regulate the skin quality, and reduce the formation of acne.

[0010] Furthermore, the medicinal part of the Viola tianshanica is one or a combination of branches, leaves, and flowers of the Viola tianshanica of the Violaceae family.

[0011] Furthermore, the extraction steps of the Viola tianshanica extract are:

[0012] Soak the medicinal parts of Viola tianshanica in petroleum ether, filter out, dry, crush and sieve;

[0013] Add alcohol to perform water bath reflux extraction, and combine the filtrates;

[0014] The filtrate is concentrated and dried to a viscous state, and then vacuum freeze-dried to obtain the Viola tianshanica extract.

[0015] Furthermore, the emulsifier is composed of lanolin, glyceryl monostearate and sorbitan monostearate, the lanolin accounts for 1% to 5% of the facial cleanser, the glyceryl monostearate accounts for 0.5% to 3% of the facial cleanser, and the sorbitan monostearate accounts for 0.5% to 3% of the facial cleanser.

[0016] The present invention adopts lanolin as an excellent emulsifier, which has strong water absorption and is not easy to become rancid; glyceryl monostearate is a non-ionic surfactant, which has both hydrophilic and lipophilic groups, has multiple functions such as wetting, emulsification, foaming, etc., and is an emulsifier; sorbitan monostearate is a surfactant, has a decontamination ability, and can also be used as an emulsifier.

[0017] Furthermore, the moisturizing agent is at least one of glycerin, sodium hyaluronate and xylitol.

[0018] Furthermore, the penetrant is at least one of isopropyl myristate and polyglutamic acid.

[0019] Furthermore, the foaming agent is one of sodium lauryl sulfate, sodium lauryl sulfate and sodium laureth sulfate.

[0020] Furthermore, the solubilizer is at least one of polyethylene glycol 400 and polysorbate 20.

[0021] The present invention provides a method for preparing the Viola tianshanica extract facial cleanser, comprising the following steps:

[0022] Prepare the components according to the composition of weight percentage;

[0023] The foaming agent and the solubilizing agent are mixed, deionized water is added, the mixture is mixed evenly, and the mixture is heated to 60° C. to 70° C. to obtain a mixture;

[0024] Add the emulsifier to the mixture, heat to 71°C to 80°C, and start cooling after it is completely dissolved and mixed evenly;

[0025] After cooling to 46℃~55℃, add the penetrant, mix well and continue cooling;

[0026] After continuing to cool to 40°C to 45°C, add the Viola tianshanica extract and the moisturizer in sequence;

[0027] Add the preservative and continue mixing;

[0028] Deionized water was added to make up the remaining volume and mixed evenly, and the temperature was lowered to 30° C. to 39° C. to obtain a facial cleanser with Viola tianshanica extract.

[0029] The present invention also provides an application of the Viola tianshanica extract facial cleanser in the preparation of anti-allergic cosmetics.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The Tianshan violet facial cleanser prepared by the present invention adopts the extract of Tianshan violet, a Xinjiang characteristic ethnic medicine, as a raw material. The Tianshan violet extract is extracted from the medicinal part of Tianshan violet, and is prepared by adding a foaming agent, a solubilizing agent, an emulsifier, a moisturizing agent, a preservative and a penetrant. It is not only mild and non-irritating, but also highly safe, has stable foaming and good cleaning effect. It can also provide additional nutrition and protection for the skin, making the skin healthier and more elastic. In addition, the product also has anti-allergic, anti-oxidant and anti-inflammatory properties, can effectively resist the damage of the external environment to the skin, slow down the aging of the skin, gently regulate the skin quality, and reduce the generation of acne. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 This is a graph showing the inhibitory effect of Viola tianshanica extract on the in vitro activity of hyaluronidase.

[0034] Figure 2 This is a comparison chart of mouse ear swelling in the anti-inflammatory experiment of Viola tianshanica extract.

[0035] Figure 3 This is a comparison chart of the cytotoxicity of Viola tianshanica extracts.

[0036] Figure 4 This is a comparison chart of the DPPH scavenging rates of Viola tianshanica extract facial cleanser and Vc. DETAILED DESCRIPTION

[0037] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0038] Example 1: A facial cleanser containing Viola tianshanica extract and a preparation method thereof.

[0039] The formula composition of a facial cleanser containing Viola tianshanica extract is shown in Table 1.

[0040] Table 1 Composition of the formula of Viola tianshanica extract facial cleanser

[0041]

[0042] A preparation method of a Viola tianshanica extract facial cleanser is as follows:

[0043] S1, soaking the medicinal part of Viola tianshanica with petroleum ether at a mass ratio of 1:20 for 1.5 hours, filtering out, drying at room temperature, and crushing through a No. 5 sieve to obtain Viola tianshanica powder. The medicinal part of Viola tianshanica is the branches, leaves and flowers of Viola tianshanica.

[0044] S2, mix the Viola tianshanica powder with 75% alcohol in a volume ratio of 1:10, extract under reflux in a 95°C water bath for 1.5 h each time, extract three times, combine the filtrates; concentrate using a rotary evaporator, dry at 60°C until viscous, freeze at -20°C for 12 h, and obtain the Viola tianshanica extract by vacuum freeze-drying.

[0045] S3, adding 10% of a foaming agent and 5% of a solubilizing agent into a reaction kettle in sequence, adding 5% of deionized water to fully dissolve, slowly stirring and heating to 65° C. to obtain a mixture, wherein the foaming agent is sodium dodecyl sulfate, and the solubilizing agent is polyethylene glycol 400;

[0046] Add emulsifiers to the mixture in sequence, heat to 75°C, stir evenly and then start cooling after being completely dissolved; the emulsifiers are composed of 3% lanolin, 2% glyceryl monostearate and 2% sorbitan monostearate;

[0047] The temperature was lowered to 50° C., 3% penetrant was added, and the temperature was continued to be lowered after stirring evenly; the penetrant was isopropyl myristate.

[0048] Continue to cool to 43°C, add 3% of Viola tianshanica extract, 50% of deionized water and 10% of moisturizing agent in sequence, wherein the moisturizing agent is glycerol;

[0049] Add 1% preservative and continue stirring, the preservative is ethyl 4-hydroxybenzoate;

[0050] Add the remaining amount of deionized water and stir evenly, cool to 35°C and discharge to obtain a facial cleanser with Viola tianshanica extract.

[0051] Example 2: A facial cleanser containing Viola tianshanica extract and a preparation method thereof.

[0052] The following experiment was conducted using the Viola tianshanica extract prepared in Example 1.

[0053] 5% of a foaming agent and 1% of a solubilizing agent are sequentially added into a reaction kettle, and 5% of deionized water is added to fully dissolve the mixture, and the mixture is slowly stirred and heated to 60° C. to obtain a mixture, wherein the foaming agent is sodium dodecyl sulfate and the solubilizing agent is polyethylene glycol 400;

[0054] Add emulsifiers to the mixture in sequence, heat to 71°C, stir evenly and then start cooling after being completely dissolved; the emulsifiers are composed of 1% lanolin, 0.5% glyceryl monostearate and 0.5% sorbitan monostearate;

[0055] Cool down to 46°C, add 0.5% penetrant and 1%, stir evenly and continue to cool down, the penetrant is isopropyl myristate;

[0056] Continue to cool to 40°C, and add 1% of Viola tianshanica extract, 50% of deionized water and 8% of moisturizing agent in sequence, wherein the moisturizing agent is glycerol;

[0057] Then add 0.1% preservative and continue stirring, the preservative is ethyl 4-hydroxybenzoate;

[0058] Add the remaining amount of deionized water and stir evenly, cool to 30°C and discharge to obtain a facial cleanser with Viola tianshanica extract.

[0059] Example 3: A facial cleanser containing Viola tianshanica extract and a preparation method thereof.

[0060] The following experiment was conducted using the Viola tianshanica extract prepared in Example 1.

[0061] 15% of a foaming agent and 7% of a solubilizing agent are sequentially added into a reaction kettle, and 5% of deionized water is added to fully dissolve, slowly stirred evenly, and heated to 70° C. to obtain a mixture, wherein the foaming agent is sodium dodecyl sulfate, and the solubilizing agent is polyethylene glycol 400;

[0062] Add emulsifiers to the mixture in sequence, heat to 80°C, stir evenly and then start cooling after being completely dissolved; the emulsifier is composed of 5% lanolin, 3% glyceryl monostearate and 3% sorbitan monostearate;

[0063] Cool down to 55°C, add 5% penetrant, stir evenly and continue to cool down, the penetrant is isopropyl myristate;

[0064] Continue to cool to 45°C, add 5% of Viola tianshanica extract, 50% of deionized water and 12% of moisturizing agent in sequence, wherein the moisturizing agent is glycerol;

[0065] Add 2% preservative and continue stirring, the preservative is ethyl 4-hydroxybenzoate;

[0066] Add the remaining amount of deionized water and stir evenly, cool to 39° C. and discharge the material to obtain a facial cleanser with Viola tianshanica extract.

[0067] The above examples 1 to 3 are specific examples of the preparation method of the Viola tianshanica extract facial cleanser of the present invention. The Viola tianshanica extract facial cleanser obtained in examples 1 to 3 is used to study the foaming property of the Viola tianshanica extract facial cleanser; the Viola tianshanica extract obtained in example 1 is used to study the anti-allergic, anti-inflammatory and cytotoxicity of the Viola tianshanica extract; the Viola tianshanica extract facial cleanser obtained in example 1 is used to conduct anti-inflammatory control tests and antioxidant tests. The relevant test methods and results are as follows:

[0068] 1. In vitro anti-allergic evaluation of Viola tianshanica extract

[0069] 1. Test methods

[0070] Referring to the Elson-Morgan hyaluronidase inhibition test, 0.1 mL of CaCl2 solution (2.5 mmol / L) and 0.5 mL of hyaluronidase solution (500 U / mL) were taken and fully shaken and maintained in a 37°C water bath for 20 min. Then, 0.5 mL of Viola tianshanica extract solution (divided into three groups of low, medium and high concentrations, with concentrations of 200 μg mL -1 , 500μg·mL -1 , 1000μg·mL -1) and shake well, and keep warm in a 37℃ water bath for 20min. Add 0.5mL sodium hyaluronate solution (0.5mg / mL), shake well, and keep warm at 37℃ for 40min; add 0.1mL NaOH solution (0.5mol / L) and 0.5mL acetylacetone solution (3mL acetylacetone dissolved in 50mL 1.0mol / L sodium carbonate solution), shake well, heat in a boiling water bath for 15min, and then immediately ice water bath for 5min; add 1.0mL Ehrlich reagent, mix well, and place at room temperature for 1h, then measure the absorbance at 530nm. The control group used acetate buffer with pH=5.6 instead of Tianshanshan extract solution.

[0071] The hyaluronidase inhibition rate was calculated as follows:

[0072] Inhibition rate = [(A-B)-(C-D)] / (A-B)×100%, where:

[0073] A—absorbance of control solution (acetic acid buffer instead of Viola tianshanica extract solution);

[0074] B—absorbance of control blank solution (acetate buffer instead of hyaluronidase solution and Viola tianshanica extract solution);

[0075] C—absorbance of sample solution;

[0076] D—Absorbance of the blank sample solution (use acetate buffer solution instead of hyaluronidase solution).

[0077] 2. Test results

[0078] The results of the inhibition rate of hyaluronic acid in low concentrations of Viola tianshanica extract are as follows Figure 1 As shown, the low, medium and high concentrations can reach 10.3%, 32.1% and 52.4% respectively. According to international standards, the inhibition rate>30% has anti-allergic activity, which proves that the medium and high concentrations of Viola tianshanica extract have certain anti-allergic activity. 1mg / mL has the pharmacological characteristics of low toxicity and anti-allergic activity, and can be used as the final dosage form.

[0079] 2. Evaluation of the anti-inflammatory effect of Viola tianshanica extract

[0080] 1. Test methods

[0081] 40 healthy Kunming mice were randomly divided into 5 groups, 8 mice in each group. They were divided into high-dose group (10g / kg), low-dose group (5g / kg), positive control drug (dexamethasone acetate) group, and blank control group (distilled water). The mice in the high-dose group, low-dose group, and blank control group were given high-dose concentrated solution of Viola tianshanica extract (500mg / mL), low-dose concentrated solution of Viola tianshanica extract (250mg / mL), and distilled water (0.2mL / 10g), respectively, and were gavaged for 7 consecutive days, once a day; the positive control drug group was intraperitoneally injected on the 7th day. 30 minutes after the last administration, 0.02 mL of xylene was evenly applied on both sides of the right ear of the mouse, and the left ear of the mouse was used as the control to observe the swelling. The mouse was killed by dislocation of the neck 30 minutes after inflammation, and ear pieces were punched in the same position of the left and right ears of the mouse with an 8mm ear puncher. The weight difference between the left and right ears of the mouse was used as the degree of ear swelling. The ear swelling inhibition rate of Viola tianshanica extract was calculated, and significance analysis was performed using SPSS data analysis software.

[0082]

[0083] 2. Test results

[0084] The mouse ear swelling degree and mouse ear swelling inhibition rate of the Viola tianshanica extract are shown in Table 2.

[0085] Table 2 Anti-inflammatory test results of Viola tianshanica extract ( n=8)

[0086]

[0087] Note: Compared with the blank control group, *P<0.05, **P<0.01; “—” indicates that there is no data for this item.

[0088] The three drug-treated groups in the experiment all showed significant differences compared with the blank control group. To clearly show the relationship between the drug resistance effects of each group, the SPSS data processing software was used to perform LSD-t test on the six groups of data, see Table 3.

[0089] Table 3 Anti-inflammatory results of Viola tianshanica extract LSD-t test results

[0090]

[0091]

[0092] Note: Comparison among multiple groups, *P<0.05, **P<0.01.

[0093] In the anti-inflammatory experiment of the present invention, the mouse ear swelling model was used to conduct the anti-inflammatory experiment on the homemade Viola tianshanica extract, and the positive drug was compared with dexamethasone acetate. Figure 3 As shown, the high-dose extract of Viola tianshanica extract had an inhibition rate of 62.50% on xylene-induced ear swelling of mice; the low-dose extract used in the experiment had an inhibition rate of 37.93% on xylene-induced ear swelling of mice. From the data shown by the inhibition rate of ear swelling of mice, it can be seen that the inhibition rate of ear swelling of high-dose and low-dose Viola tianshanica extracts is better than the anti-inflammatory effect of the blank group.

[0094] In summary, the experiment proves that the extract of Viola tianshanica has anti-inflammatory effect. And within the dosage range (5g / kg~10g / kg), the anti-inflammatory effect of the extract of Viola tianshanica may increase with the increase of dosage.

[0095] 3. Evaluation of in vitro cytotoxicity of Viola tianshanica extract

[0096] 1. Test methods

[0097] RBL-2H3 cells in the logarithmic growth phase were digested with trypsin and resuspended in MEM basal medium. The cells were counted and the cell concentration was adjusted to 5×10 3 mL -1 , inoculated in 96-well plates, 100 μL per well, 5% CO2, 37°C culture until the cells are stable in the adherent state. After 24 hours, the supernatant was discarded and samples of various concentrations (the highest concentration was the mother solution, i.e., 10 mg·mL of Viola tianshanica extract) were added. -1 , diluted to 2×10 3 μg·mL -1 , 1×10 3 μg·mL -1 , 500μg·mL -1 , 200 μg·mL -1 , 100 μg·mL -1 , 50μg·mL -1 , 20μg·mL -1 , 10μg·mL -1 ), set up blank control (10% purified water) and negative control (MEM medium). After 24 hours, discard the supernatant, add 10% (v / v) CCK8 solution, measure the absorbance at 450nm after 1 hour of reaction, and calculate the cell viability of each group. Cell viability (%) = (A T -A C )( / A N -A C )

[0098] Wherein: AT: absorbance value of the well containing cells + CCK8 + sample solution; AC: absorbance value of the well containing CCK8 + MEM culture medium; AN: absorbance value of the well containing cells + CCK8 + MEM culture medium.

[0099] 2. Test results

[0100] The results of cell viability after administration are as follows Figure 3 As shown, the extract of Viola tianshanica at 10 μg mL -1 ~200 μg mL -1 Almost no cytotoxicity, even at 200 μg mL -1 There was a trend of promoting cell growth until 1 mg·mL -1 It then showed a certain degree of cytotoxicity. The dosage concentration in the facial cleanser is 3%, which is within the safe dosage range.

[0101] 4. Evaluation of the foaming performance of the facial cleanser containing Viola tianshanica extract

[0102] The amount of foam is an important indicator of cleaning ability. When the facial cleanser comes into contact with the skin, the hydrophobic end of the surfactant in the water will combine with the dirt, wash the dirt off the skin surface, and then be washed away with water, thereby achieving the cleaning effect. Therefore, the right amount of foam can exert better cleaning ability. Next, the foaming performance of the three embodiments will be evaluated.

[0103] 1. Test methods

[0104] The foam test method is in accordance with GB / T 29679-2013 "Shampoo, Shampoo Cream". Preparation of facial cleanser sample solution: weigh 1g of Tianshan Viola extract facial cleanser in a 500ml beaker, add 450ml deionized water and 50ml hard water, stir evenly with a glass rod, and heat to (40±1)℃ in a digital constant temperature water bath (Jiangsu Kexi Instrument Co., Ltd.) for use. Use Roche foam meter to test the foam height of the facial cleanser samples of Examples 1 to 3 at 0min, 1min and 5min.

[0105] 2. Test results

[0106] The test results are shown in Table 4.

[0107] Table 4 Stability determination of Viola tianshanica extract facial cleanser

[0108] 0min foam height Foam height after 1 minute Foam height after 5 minutes Example 1 52 51 48 Example 2 46 44 40 Example 3 53 50 47

[0109] As shown in Table 2, the facial cleansers prepared in Examples 1 to 3 have good foaming properties and foam stability. The foam height of the facial cleansers can reach 50 mm, the foam is dense, and the foaming property is good. After foaming for 5 minutes, the foam decreases slightly, indicating that the foam stability is good.

[0110] 5. Anti-inflammatory control experiment of Tianshan Viola extract facial cleanser

[0111] 1. Test methods

[0112] A total of 200 volunteers, including computer workers, those with mild acne and outdoor workers, were recruited and randomly divided into 5 groups, 40 people in each group, 4 experimental groups and 1 control group. The control group was given a facial cleanser containing coenzyme Q10 (active ingredient sodium lauryl sulfate) purchased from a supermarket, which was used once in the morning and once in the evening for 3 consecutive months.

[0113] Significant improvement: The patient's skin inflammation was significantly improved, and the inflamed area was significantly reduced;

[0114] Relief: Feeling more comfortable after use, but no significant improvement was found;

[0115] Ineffective: There is almost no change before and after use;

[0116] Aggravation: Symptoms of enlargement of the inflamed area before and after use.

[0117] 2. Test results

[0118] Table 5 The efficacy of Viola tianshanica extract facial cleanser in relieving skin inflammation

[0119]

[0120] The results show that the number of computer workers, mild acne, and outdoor workers who use the Viola tianshanica extract facial cleanser for a long time is significantly improved, which is about 7 times the number of people who use the coenzyme Q10 facial cleanser alone.

[0121] VI. Evaluation of the Antioxidant Activity of Viola tianshanica Extract Facial Cleanser

[0122] 1. Test methods

[0123] Prepare the vitamin C solution into a mother solution with a concentration of 600μg / mL, and dilute it to 7.5μg / mL, 15μg / mL, 30μg / mL, 60μg / mL, 120μg / mL, 150μg / mL, 300μg / mL, and 450μg / mL for testing in subsequent experiments; prepare a 0.6mg / mL DPPH alcohol solution as the mother solution, store it in a dark place, and dilute it to 30μg / mL with ethanol before use. Prepare the Tianshan Viola extract into a solution with the same concentration as vitamin C for testing. Take 1mL of the test sample solution, add 4mL of DPPH alcohol solution, mix well, react for 60min in a dark place, and use a UV spectrophotometer to measure the absorbance at 517nm. Make three parallel samples for each sample, use ethanol as a blank control, and the test results are expressed as the clearance rate Q. The test results are expressed as DPPH clearance rate:

[0124]

[0125] 2. Test results

[0126] The scavenging effect of DPPH free radicals in the facial cleanser made from Viola tianshanica extract is shown in Figure 4 The results showed that the scavenging rate of DPPH free radicals increased from 1.98% to 47.75% when the concentration of Viola tianshanica extract increased from 7.5μg / mL to 600μg / mL, and the scavenging rate of DPPH free radicals increased from 13.22% to 98.16% when the concentration of vitamin C increased from 7.5μg / mL to 600μg / mL. The scavenging rate of Viola tianshanica extract facial cleanser at a concentration of 600μg / mL was not much different from that of vitamin C, reaching 48.65% of vitamin C, indicating that Viola tianshanica extract facial cleanser has a good free radical scavenging effect at this concentration.

[0127] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0128] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents,

[0129] The present invention is intended to include these modifications and variations.

Claims

1. A facial cleanser containing Viola tianshanica extract, characterized in that: The invention is prepared from the following components in weight percentage: 2% to 8% of effective active ingredient, 5% to 15% of foaming agent, 1% to 7% of solubilizer, 2% to 11% of emulsifier, 8% to 12% of moisturizer, 0.1% to 2% of preservative, 0.5% to 5% of penetrant, and the balance is deionized water; Wherein, the effective active ingredient is composed of Viola tianshanica extract, and the Viola tianshanica extract accounts for 1% to 5% of the Viola tianshanica extract facial cleanser; The Viola tianshanica extract is obtained by extracting the medicinal part of Viola tianshanica through ethanol.

2. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that The medicinal parts of the Viola tianshanica are one or a combination of branches, leaves and flowers of the Viola tianshanica.

3. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The extraction steps of the Viola tianshanica extract are: Soak the medicinal parts of Viola tianshanica in petroleum ether, filter out, air dry, crush and sieve; Add alcohol to perform water bath reflux extraction, and combine the filtrates; The filtrate is concentrated and dried to a viscous state, and then vacuum freeze-dried to obtain the Viola tianshanica extract.

4. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that The emulsifier is composed of lanolin, glyceryl monostearate and sorbitan monostearate, wherein the lanolin accounts for 1% to 5% by weight of the Viola tianshanica extract facial cleanser, the glyceryl monostearate accounts for 0.5% to 3% by weight of the Viola tianshanica extract facial cleanser, and the sorbitan monostearate accounts for 0.5% to 3% by weight of the Viola tianshanica extract facial cleanser.

5. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The moisturizing agent is at least one of glycerin, sodium hyaluronate and xylitol.

6. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The penetrant is at least one of isopropyl myristate and polyglutamic acid.

7. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The foaming agent is at least one of sodium lauryl sulfate, sodium lauryl sulfate and sodium laureth sulfate.

8. The Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The solubilizer is at least one of polyethylene glycol 400 and polysorbate 20.

9. A method for preparing the Viola tianshanica extract facial cleanser according to claim 1, characterized in that: The steps include: Prepare the components according to the composition of weight percentage; The foaming agent and the solubilizing agent are mixed, deionized water is added, the mixture is mixed evenly, and the mixture is heated to 60° C. to 70° C. to obtain a mixture; Add the emulsifier to the mixture, heat to 71°C to 80°C, and start cooling after it is completely dissolved and mixed evenly; After cooling to 46℃~55℃, add penetrant, mix well and continue cooling; After continuing to cool to 40°C to 45°C, add the Viola tianshanica extract and the moisturizer in sequence; Add the preservative and continue mixing; Deionized water was added to make up the remaining volume and mixed evenly, and the temperature was lowered to 30° C. to 39° C. to obtain a facial cleanser with Viola tianshanica extract.

10. Use of the Viola tianshanica extract facial cleanser according to claim 1 in the preparation of anti-allergic cosmetics.

Images