Anti-wrinkle and whitening composition containing North American red maple leaf extract as well as preparation method and application of anti-wrinkle and whitening composition

Abstract

The invention provides an anti-wrinkle and whitening composition containing North America red maple leaf extract as well as a preparation method and application of the anti-wrinkle and whitening composition, and belongs to the technical field of washing and chemical products. The composition disclosed by the invention is prepared from the following components in parts by weight: 30 to 50 parts of North America red maple leaf extract fermented filtrate, 20 to 45 parts of cerasus humilis fruit extract, 10 to 18 parts of hydroxyl pinacolone retinoate and 10 to 18 parts of tocopherol. The North America red maple leaf extract fermented filtrate, the cerasus humilis fruit extract, the hydroxypinacolone retinoate and the tocopherol are coordinated and matched, so that the reproduction of harmful bacteria on the skin surface can be effectively inhibited, the skin is protected from being damaged by the harmful bacteria, the skin barrier is repaired, and the stability of the micro-ecology on the skin surface is maintained; meanwhile, generation of skin melanin can be effectively inhibited, the antioxidant performance of the skin is improved, metabolism of the skin is promoted, the moisture content of the skin is increased, generation of wrinkles is inhibited, and the remarkable anti-wrinkle and whitening effects are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cleaning products, and in particular relates to an anti-wrinkle and whitening composition containing a North American red maple leaf extract, and a preparation method and application thereof. Background Art

[0002] Skin is the natural barrier and outer garment of the human body. It refers to the tissue covering the surface of the human body and directly contacting the external environment. It has the functions of protection, sensation, secretion, excretion, and respiration. It is formed by the close combination of the epidermis and the dermis. The epidermis is composed of stratum corneum, which is divided into the stratum lucidum, the stratum granulosum, and the stratum germinativum from shallow to deep. The stratum corneum is composed of multiple layers of keratinized epithelial cells (nuclei and organelles disappear, and the cell membrane is thicker). It is lifeless and impermeable. It has the functions of preventing the outflow of tissue fluid, anti-friction and anti-infection. The cells of the stratum germinativum continue to proliferate and gradually migrate outward to replenish the stratum corneum that is constantly falling off. The stratum germinativum contains melanocytes that can produce melanin. Melanin is scattered between basal cells. The depth of skin color is determined by the number of melanin particles. In addition, the skin also stores a large amount of water, protein, fat, sugar and vitamins, which participate in the metabolic process of the whole body. When the metabolism of the body is affected, it will cause skin problems such as dry skin, dull skin and aging.

[0003] With the rapid development of economy and the improvement of people's living standards, people pay more attention to skin care. Skin care, especially facial skin care, has become extremely important in daily life. However, many skin care products on the market have relatively single functions, and people have higher and higher requirements for functional skin care products. They hope to solve various skin problems through skin care products with multiple functions. Therefore, it is still necessary to develop products with both whitening and anti-wrinkle effects. Summary of the invention

[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide an anti-wrinkle and whitening composition and a preparation method and application thereof. The prepared composition can regulate the skin microecology and achieve good anti-wrinkle and whitening effects.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides an anti-wrinkle and whitening composition, which comprises the following components by weight: 30 to 50 parts of fermentation filtrate of North American red maple leaf extract, 20 to 45 parts of European plum fruit extract, 10 to 18 parts of hydroxypinacolone retinoic acid ester and 10 to 18 parts of tocopherol.

[0007] Preferably, the method for preparing the fermentation filtrate of the North American red maple leaf extract comprises the following steps: drying and crushing the North American red maple leaves, mixing them with water, loading them into an ultrasonic extraction kettle, and ultrasonically extracting them under airlift stirring conditions to obtain an extract; adding galacto-oligosaccharides to the extract to obtain a mixture, adding yeast to the mixture for fermentation to obtain a fermentation liquid; sterilizing and centrifuging the fermentation liquid, and taking the supernatant to obtain the fermentation filtrate of the North American red maple leaf extract.

[0008] Preferably, the North American red maple leaf is mixed with water at a mass volume ratio of 1 g: (10-30) mL; the temperature of the ultrasonic extraction is 25-45° C., and the time of the ultrasonic extraction is 15-45 min.

[0009] Preferably, based on the mass of the extract, the amount of galacto-oligosaccharide added is 1-5%, and the amount of yeast added is 1×10 8 ~9×10 9 cfu / kg; the fermentation time is 10 to 30 hours, and the fermentation temperature is 30 to 40°C.

[0010] Preferably, the preparation method of the Prunus cerasifera fruit extract comprises the following steps: crushing the Prunus cerasifera fruit and mixing it with ethanol, heating and refluxing for extraction to obtain a Prunus cerasifera fruit extract, filtering, concentrating, and freeze-drying the Prunus cerasifera fruit extract to obtain a Prunus cerasifera fruit extract.

[0011] Preferably, the European holly fruit and ethanol are mixed at a ratio of 10 g: (100-120) mL; and the heating reflux extraction time is 4-6 h.

[0012] Preferably, the following components are also included, by weight: 10 to 15 parts of a penetration enhancer, 0.2 to 0.8 parts of a thickener, 3 to 9 parts of a humectant, and 80 to 100 parts of deionized water.

[0013] The present invention also provides a method for preparing the anti-wrinkle and whitening composition, comprising the following steps: heating deionized water to 60-80° C., adding fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol, mixing evenly, then adding a penetration enhancer and a moisturizer, stirring for 30-50 minutes; cooling to 40-50° C., then adding a thickener, stirring for 30-50 minutes; and cooling to room temperature.

[0014] The present invention also provides the use of the anti-wrinkle and whitening composition in the preparation of anti-wrinkle and / or whitening products, and the products include skin care products, cosmetics, and cleaning products.

[0015] The present invention also provides an anti-wrinkle and / or whitening product, which comprises the anti-wrinkle and whitening composition, wherein the content of the anti-wrinkle and whitening composition is 10-100wt%.

[0016] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0017] The present invention coordinates and matches North American red maple leaf extract fermentation filtrate, European prune fruit extract, hydroxypinacolone retinoic acid ester and tocopherol, which can effectively inhibit the reproduction of Staphylococcus aureus, Propionibacterium acnes, Malassezia and Candida albicans on the skin surface, protect the skin from damage by harmful bacteria, repair the skin barrier and maintain the stability of the microecology on the skin surface; at the same time, the North American red maple leaf extract fermentation filtrate, European prune fruit extract, hydroxypinacolone retinoic acid ester and tocopherol can effectively inhibit the generation of skin melanin, improve the skin's antioxidant performance, promote skin metabolism, increase the skin's moisture content and achieve significant anti-wrinkle and whitening effects. DETAILED DESCRIPTION

[0018] The present invention provides an anti-wrinkle and whitening composition, which comprises the following components by weight: 30 to 50 parts of fermentation filtrate of North American red maple leaf extract, 20 to 45 parts of European prune fruit extract, 10 to 18 parts of hydroxypinacolone retinoic acid ester and 10 to 18 parts of tocopherol. Preferably, the anti-wrinkle and whitening composition comprises the following components by weight: 35 to 45 parts of fermentation filtrate of North American red maple leaf extract, 25 to 40 parts of European prune fruit extract, 12 to 16 parts of hydroxypinacolone retinoic acid ester and 12 to 16 parts of tocopherol.

[0019] The preparation method of the fermentation filtrate of the North American red maple leaf extract of the present invention comprises the following steps: drying and crushing the North American red maple leaf, mixing with water, loading into an ultrasonic extraction kettle, and ultrasonically extracting under airlift stirring conditions to obtain an extract; adding galacto-oligosaccharide to the extract to obtain a mixture, adding yeast to the mixture for fermentation to obtain a fermentation liquid; sterilizing and centrifuging the fermentation liquid, taking the supernatant to obtain the fermentation filtrate of the North American red maple leaf extract.

[0020] The North American red maple leaf of the present invention is a fresh leaf of a red maple tree, and the varieties of the North American red maple leaf include autumn flame, October brilliance, sugar maple, autumn tower, Mamo autumn maple, free maple, sunset red, sun valley, Shanna Green red maple, Armstrong, etc. The North American red maple leaf of the present invention is cleaned of surface soil and dust and is ready for use. The present invention pulverizes the North American red maple leaf after drying, and the drying is preferably carried out by a two-stage drying method, that is, the North American red maple leaf is dried at 50-65°C for 0.5-1h, then cooled at room temperature for 0.5-0.6h, and then dried at 110-130°C for 1.5-2h. The present invention can obtain dried North American red maple leaves by a two-stage drying method, while avoiding excessive loss of active ingredients of the North American red maple leaves by a high-temperature drying. The present invention pulverizes the dried North American red maple leaves, preferably pulverizing to 50-100 meshes. The present invention mixes North American red maple leaf powder with water, and the water is preferably deionized water. The North American red maple leaf and water are mixed at a mass volume ratio of 1g: (10-30) mL, preferably at a mass volume ratio of 1g: (15-25) mL. The temperature of the ultrasonic extraction of the present invention is 25-45°C, preferably 30-35°C; the time of the ultrasonic extraction is 15-45min, preferably 20-35min. The ultrasonic generation mode is self-excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power of 300W, ultrasonic frequency of 22-55kHz, and extraction mode is ultrasonic once every 2min, each time for 3min.

[0021] The present invention adds galacto-oligosaccharide to the extract after ultrasonic extraction to obtain a mixture, and the addition amount of the galacto-oligosaccharide is 1-5%, preferably 2-4%, based on the mass of the extract; the addition of galacto-oligosaccharide in the present invention provides a carbon source for the growth metabolism of yeast, promotes the fermentation process of the North American red maple leaf extract by yeast, and on the other hand, the galacto-oligosaccharide is degraded into small molecule sugars through the growth metabolism of yeast, provides prebiotics for the beneficial bacteria on the surface of the skin, and has the effect of maintaining the stability of the microecological flora on the surface of the skin. The addition amount of the yeast in the present invention is 1×10 8 ~9×10 9 cfu / kg; the fermentation time is 10 to 30 hours, preferably 15 to 25 hours; the fermentation temperature is 30 to 40°C, preferably 35 to 37°C.

[0022] The present invention sterilizes and centrifuges the fermentation liquid. The sterilization is preferably high temperature sterilization, and the sterilization conditions are 100-110°C for 10-15 minutes; the centrifugation conditions are preferably 2000-8000r / min for 5-10 minutes. After centrifugation, the supernatant is taken to obtain the fermentation filtrate of the North American red maple leaf extract.

[0023] The fermentation filtrate of the North American red maple leaf extract obtained by the invention has an inhibitory effect on tyrosinase, can inhibit the proliferation and differentiation of melanocytes, reduce the synthesis and transport of melanosomes, and thus reduce the deposition of melanin in the skin; at the same time, the flavonoid compounds, polyphenols and the like in the fermentation filtrate of the North American red maple leaf extract can effectively remove free radicals in the skin, reduce the damage of free radicals to the skin, promote the metabolism of keratinocytes, accelerate the shedding of aged keratinocytes, and inhibit the formation of wrinkles, thereby achieving the effects of delaying skin aging, anti-wrinkle and whitening.

[0024] The preparation method of the Prunus cerasifera fruit extract of the present invention comprises the following steps: crushing the Prunus cerasifera fruit, mixing with ethanol, heating and refluxing for extraction to obtain the Prunus cerasifera fruit extract, filtering, concentrating and freeze-drying the Prunus cerasifera fruit extract to obtain the Prunus cerasifera fruit extract.

[0025] Prunus humilis Bunge is a deciduous shrub plant of the genus Prunus in the Rosaceae family. The Prunus humilis Bunge fruit of the present invention is the fresh fruit of Prunus humilis Bunge. The Prunus humilis Bunge fruit is crushed after cleaning the dust on the surface. The crushing process is preferably to use a crusher to crush the fruit pulp to a particle size of 3 to 6 mm. The crushed Prunus humilis Bunge fruit is mixed with ethanol according to 10g: (100-120) mL, preferably 10g: 110 mL; the ethanol is preferably 80-90% ethanol solution, and the heating reflux extraction time is 4 to 6h, preferably 5h. The Prunus humilis Bunge fruit extract is filtered and concentrated by the present invention. The concentration is preferably concentrated to an extract at 45°C and concentrated to a relative density of 1.0 to 1.3g / mL. The extract is freeze-dried to obtain the Prunus humilis Bunge fruit extract, and the freeze-drying is preferably vacuum freeze-drying. As an optional embodiment, the freeze-drying step is: pre-freezing at -80°C for 2 to 4 hours, and then vacuum freeze-drying at -60 to -40°C for 20 to 24 hours to obtain a freeze-dried product, which is the European plum fruit extract.

[0026] The hollyhock fruit extract obtained by the present invention has good antibacterial effect, can effectively inhibit the reproduction of harmful bacteria such as Staphylococcus aureus, Propionibacterium acnes, Malassezia and Candida albicans on the skin surface, and promote the secretion of antimicrobial polypeptide substances (AMPs) by the small sweat glands on the skin surface, thereby protecting the skin from damage by harmful bacteria, repairing the skin barrier, and maintaining the stability of the microecological flora on the skin surface.

[0027] The hydroxypinacolone retinoic acid ester (HPR) of the present invention is a highly effective ingredient that directly acts on the skin, can promote procollagen synthesis and reduce skin inflammation. Tocopherol, also known as vitamin E, can inhibit tyrosinase activity and has good antioxidant properties. The present invention uses hydroxypinacolone retinoic acid ester and tocopherol in combination with North American red maple leaf extract fermentation filtrate and European plum fruit extract, which can show better performance in skin anti-aging, whitening and anti-irritation.

[0028] The anti-wrinkle and whitening composition of the present invention preferably further comprises 10 to 15 parts of a penetration enhancer, 0.2 to 0.8 parts of a thickener, 3 to 9 parts of a moisturizer, and 80 to 100 parts of deionized water. More preferably, it further comprises 12 to 14 parts of a penetration enhancer, 0.4 to 0.6 parts of a thickener, 4 to 6 parts of a moisturizer, and 85 to 90 parts of deionized water.

[0029] The penetration enhancer of the present invention includes but is not limited to any one or more of ethanol, propylene glycol, nicotinamide and azone; the thickener includes but is not limited to any one or more of carbomer, sodium carrageenan, sodium alginate, xanthan gum and coconut oil thickener; the moisturizer includes but is not limited to any one or more of glycerin, panthenol, ceramide, erythritol, hyaluronic acid, xylitol and betaine. The penetration enhancer, thickener and moisturizer of the present invention can be purchased conventionally.

[0030] The present invention also provides a method for preparing the above anti-wrinkle and whitening composition, comprising the following steps: heating deionized water to 60-80°C, adding fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol, mixing evenly, then adding a penetration enhancer and a moisturizer, stirring for 30-50 minutes; cooling to 40-50°C, then adding a thickener, stirring for 30-50 minutes; cooling to room temperature. The deionized water is preferably heated to 70-75°C, and the stirring time is preferably 35-40 minutes.

[0031] The present invention also provides the use of the anti-wrinkle and whitening composition in the preparation of anti-wrinkle and / or whitening products, including skin care products, cosmetics, and cleaning products. The anti-wrinkle and / or whitening product of the present invention uses the anti-wrinkle and whitening composition as the main active ingredient, and those skilled in the art can add other auxiliary materials (such as flavoring agents, conditioning agents, etc.) according to the type of specific products.

[0032] The present invention also provides an anti-wrinkle and / or whitening product, which comprises the anti-wrinkle and whitening composition, wherein the content of the anti-wrinkle and whitening composition is 10 to 100 wt %, preferably 70 to 90 wt %.

[0033] The technical solutions in the present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0035] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0036] Example 1

[0037] An anti-wrinkle and whitening composition is obtained by mixing 40 parts of North American red maple leaf extract fermentation filtrate, 35 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, by weight.

[0038] Preparation of North American red maple leaf extract fermentation filtrate:

[0039] (1) Clean the dust from the surface of North American red maple leaves (Autumn Flame), dry them at 60°C for 0.5 h, cool them at room temperature for 0.5 h, dry them at 120°C for 1.5 h, and grind them into 100 mesh to obtain North American red maple leaf powder.

[0040] (2) The North American red maple leaf powder and water were mixed in a mass volume ratio of 1 g: 25 mL, and ultrasonic extraction was performed at 35°C for 30 min. The ultrasonic generation method was excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power of 300 W, ultrasonic frequency of 35 kHz, and the extraction method was ultrasonic once every 2 min, each time for 3 min, to obtain the North American red maple leaf extract.

[0041] (3) Add 3 wt% galacto-oligosaccharide to the North American red maple leaf extract, mix and then add 1×10 yeast 9 cfu / kg (based on the mass of North American red maple leaf extract), yeast was purchased from Angel Yeast Co., Ltd., Angel semi-dry yeast. Fermentation was carried out at 35°C for 25h to obtain a fermentation liquid.

[0042] (4) The fermentation liquid was sterilized at 105° C. for 10 min and centrifuged at 5000 rpm for 8 min. The supernatant obtained by centrifugation was the fermentation filtrate of the North American red maple leaf extract.

[0043] Preparation of Prunus armeniaca Fruit Extract:

[0044] (1) Wash the surface dust of the European plum fruit and crush it with a sawtooth crusher. The particle size of the fruit pulp is 5 mm.

[0045] (2) The crushed European holly fruit was mixed with 85% ethanol solution at a ratio of 10 g:110 mL, and the mixture was heated under reflux for 5 h to obtain European holly fruit extract.

[0046] (3) The extract of Prunus cerasifera fruit is concentrated into an extract at 45°C to a relative density of 1.12 g / mL; the extract is pre-frozen at -80°C for 3 h, and then vacuum freeze-dried at -40°C for 22 h to obtain a lyophilized product, which is the Prunus cerasifera fruit extract.

[0047] Example 2

[0048] An anti-wrinkle and whitening composition is obtained by mixing 30 parts of fermentation filtrate of North American red maple leaf extract, 20 parts of European prune fruit extract, 10 parts of hydroxypinacolone retinoic acid ester and 10 parts of tocopherol, by weight.

[0049] Preparation of North American red maple leaf extract fermentation filtrate:

[0050] (1) Clean the dust from the surface of the North American red maple leaf (October Splendor), dry it at 50°C for 0.5 h, cool it at room temperature for 0.5 h, dry it at 110°C for 1.5 h, and grind it into 50 mesh to obtain the North American red maple leaf powder.

[0051] (2) The North American red maple leaf powder and water were mixed in a mass volume ratio of 1 g: 10 mL, and ultrasonic extraction was performed at 25°C for 45 min. The ultrasonic generation method was excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power was 300 W, ultrasonic frequency was 22 kHz, and the extraction method was ultrasonic once every 2 min, each time for 3 min, to obtain the North American red maple leaf extract.

[0052] (3) Add 1 wt% galacto-oligosaccharide to the North American red maple leaf extract, mix and then add 1×10 yeast 8 cfu / kg (based on the mass of North American red maple leaf extract), yeast was purchased from Angel Yeast Co., Ltd., Angel semi-dry yeast. Fermentation was carried out at 30°C for 30 hours to obtain a fermentation liquid.

[0053] (4) The fermentation liquid was sterilized at 100° C. for 15 min and centrifuged at 3000 rpm for 10 min. The supernatant obtained by centrifugation was the fermentation filtrate of the North American red maple leaf extract.

[0054] Preparation of Prunus armeniaca Fruit Extract:

[0055] (1) Wash the surface dust of the European plum fruit and crush it with a sawtooth crusher. The particle size of the fruit pulp is 6 mm.

[0056] (2) The crushed European prune fruit was mixed with 90% ethanol solution at a ratio of 10 g:100 mL, and the mixture was heated under reflux for 4 h to obtain European prune fruit extract.

[0057] (3) The extract of Prunus cerasifera fruit is concentrated into an extract at 45°C to a relative density of 1.21 g / mL; the extract is pre-frozen at -80°C for 2 h, and then vacuum freeze-dried at -60°C for 20 h to obtain a lyophilized product, which is the Prunus cerasifera fruit extract.

[0058] Example 3

[0059] An anti-wrinkle and whitening composition is obtained by mixing 50 parts of fermentation filtrate of North American red maple leaf extract, 45 parts of European prune fruit extract, 18 parts of hydroxypinacolone retinoic acid ester and 18 parts of tocopherol, calculated by weight.

[0060] Preparation of North American red maple leaf extract fermentation filtrate:

[0061] (1) Clean the dust from the surface of North American red maple leaves (sunset red), dry them at 65°C for 1 h, cool them at room temperature for 0.6 h, dry them at 130°C for 2 h, and grind them into 100 mesh to obtain North American red maple leaf powder.

[0062] (2) The North American red maple leaf powder and water were mixed in a mass volume ratio of 1 g: 30 mL, and ultrasonic extraction was performed at 45°C for 15 min. The ultrasonic generation method was excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power of 300 W, ultrasonic frequency of 55 kHz, and the extraction method was ultrasonic once every 2 min, each time for 3 min, to obtain the North American red maple leaf extract.

[0063] (3) Add 5 wt% galacto-oligosaccharide to the North American red maple leaf extract, mix and then add 9×10 yeast 9 cfu / kg (based on the mass of North American red maple leaf extract), yeast was purchased from Angel Yeast Co., Ltd., Angel semi-dry yeast. Fermentation was carried out at 40°C for 10 hours to obtain a fermentation liquid.

[0064] (4) The fermentation liquid was sterilized at 110° C. for 10 min and centrifuged at 6000 rpm for 5 min. The supernatant obtained by centrifugation was the fermentation filtrate of the North American red maple leaf extract.

[0065] Preparation of Prunus armeniaca Fruit Extract:

[0066] (1) Wash the surface dust of European plum fruit and crush it with sawtooth crusher. The particle size of the fruit pulp is 3mm.

[0067] (2) The crushed European holly fruit was mixed with 80% ethanol solution at a ratio of 10 g:120 mL, and the mixture was heated under reflux for 6 h to obtain European holly fruit extract.

[0068] (3) The extract of Prunus cerasifera fruit is concentrated into an extract at 45°C to a relative density of 1.14 g / mL; the extract is pre-frozen at -80°C for 4 hours, and then vacuum freeze-dried at -40°C for 24 hours to obtain a lyophilized product, which is the Prunus cerasifera fruit extract.

[0069] Comparative Example 1

[0070] An anti-wrinkle and whitening composition is obtained by mixing 35 parts of European holly fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, calculated by weight.

[0071] The preparation method of the Prunus cerasifera fruit extract is the same as that in Example 1.

[0072] Comparative Example 2

[0073] An anti-wrinkle and whitening composition is obtained by mixing 40 parts of North American red maple leaf extract fermentation filtrate, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, calculated by weight.

[0074] The preparation method of the fermentation filtrate of North American red maple leaf extract is the same as that in Example 1.

[0075] Comparative Example 3

[0076] An anti-wrinkle and whitening composition is obtained by mixing 40 parts of fermentation filtrate of North American red maple leaf extract, 35 parts of European plum fruit extract, 12 parts of retinol and 12 parts of tocopherol, calculated by weight.

[0077] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0078] Comparative Example 4

[0079] An anti-wrinkle and whitening composition is obtained by mixing 20 parts of fermentation filtrate of North American red maple leaf extract, 55 parts of European plum fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, calculated by weight.

[0080] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0081] Comparative Example 5

[0082] An anti-wrinkle and whitening composition is obtained by mixing 70 parts of North American red maple leaf extract fermentation filtrate, 5 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, by weight.

[0083] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0084] Comparative Example 6

[0085] An anti-wrinkle and whitening composition is obtained by mixing 40 parts of North American red maple leaf extract, 35 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, by weight.

[0086] Preparation of North American red maple leaf extract:

[0087] (1) Clean the dust from the surface of North American red maple leaves (Autumn Flame), dry them at 60°C for 0.5 h, cool them at room temperature for 0.5 h, dry them at 120°C for 1.5 h, and grind them into 100 mesh to obtain North American red maple leaf powder.

[0088] (2) Red maple leaf powder and water were mixed at a mass volume ratio of 1g:25mL, and ultrasonic extraction was performed at 35°C for 30min. The ultrasonic generation method was excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power of 300W, ultrasonic frequency of 35kHz, and extraction method was ultrasonic once every 2min, each time for 3min. After ultrasonic extraction, the supernatant was filtered and the red maple leaf extract was obtained.

[0089] The preparation of the Prunus armeniaca fruit extract is the same as in Example 1.

[0090] Comparative Example 7

[0091] An anti-wrinkle and whitening composition is obtained by mixing 40 parts of North American red maple leaf extract fermentation filtrate, 35 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester and 12 parts of tocopherol, by weight.

[0092] Preparation of North American red maple leaf extract fermentation filtrate:

[0093] (1) Clean the dust from the surface of North American red maple leaves (Autumn Flame), dry them at 60°C for 0.5 h, cool them at room temperature for 0.5 h, dry them at 120°C for 1.5 h, and grind them into 100 mesh to obtain North American red maple leaf powder.

[0094] (2) The North American red maple leaf powder and water were mixed in a mass volume ratio of 1 g: 25 mL, and ultrasonic extraction was performed at 35°C for 30 min. The ultrasonic generation method was excitation ultrasonic generation, radial vibration cylindrical transducer, ultrasonic power of 300 W, ultrasonic frequency of 35 kHz, and the extraction method was ultrasonic once every 2 min, each time for 3 min, to obtain the North American red maple leaf extract.

[0095] (3) Add 1×10 yeast to the North American red maple leaf extract 9 cfu / kg (based on the mass of North American red maple leaf extract), yeast was purchased from Angel Yeast Co., Ltd., Angel semi-dry yeast. Fermentation was carried out at 35°C for 25h to obtain a fermentation liquid.

[0096] (4) The fermentation liquid was sterilized at 105° C. for 10 min and centrifuged at 5000 rpm for 8 min. The supernatant obtained by centrifugation was the fermentation filtrate of the North American red maple leaf extract.

[0097] The preparation of Prunus armeniaca fruit extract is the same as in Example 1.

[0098] Example 4

[0099] An anti-wrinkle and whitening composition, calculated by weight, comprises: 40 parts of fermentation filtrate of North American red maple leaf extract, 35 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0100] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0101] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 70°C, and fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol are added, and mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 35 minutes; the temperature is lowered to 45°C, and a thickener is added, and stirred for 35 minutes; and the mixture is cooled to room temperature.

[0102] Example 5

[0103] An anti-wrinkle and whitening composition comprises, by weight, 45 parts of fermentation filtrate of North American red maple leaf extract, 25 parts of European prune fruit extract, 10 parts of hydroxypinacolone retinoic acid ester, 10 parts of tocopherol, 15 parts of a penetration enhancer, 0.2 parts of a thickener, 3 parts of a moisturizer, and 80 parts of deionized water. The penetration enhancer is a mixture of propylene glycol and nicotinamide in equal proportions, the thickener is carbomer, and the moisturizer is a mixture of glycerin and panthenol in equal proportions.

[0104] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0105] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 60°C, and fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol are added, and mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 30 minutes; the temperature is lowered to 40°C, and a thickener is added, and stirred for 30 minutes; and the mixture is cooled to room temperature.

[0106] Example 6

[0107] An anti-wrinkle and whitening composition, which comprises, by weight, 50 parts of fermentation filtrate of North American red maple leaf extract, 45 parts of European plum fruit extract, 18 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of a penetration enhancer, 0.8 parts of a thickener, 9 parts of a moisturizer, and 100 parts of deionized water. The penetration enhancer is nicotinamide, the thickener is a mixture of sodium alginate and coconut oil thickener in equal proportions, and the moisturizer is a mixture of panthenol, erythritol, and hyaluronic acid in equal proportions.

[0108] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0109] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 80°C, fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol are added, and mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 50 minutes; the temperature is lowered to 50°C, and a thickener is added, and stirred for 50 minutes; and the mixture is cooled to room temperature.

[0110] Example 7

[0111] An anti-wrinkle and whitening composition, which comprises, by weight, 45 parts of fermentation filtrate of North American red maple leaf extract, 32 parts of European prune fruit extract, 14 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of a penetration enhancer, 0.6 parts of a thickener, 5 parts of a moisturizer, and 95 parts of deionized water. The penetration enhancer is nicotinamide, the thickener is sodium carrageenan, and the moisturizer is a mixture of glycerin, erythritol, hyaluronic acid, and xylitol in equal proportions.

[0112] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0113] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 65°C, and fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol are added, and mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 40 minutes; the temperature is lowered to 45°C, and a thickener is added, and stirred for 35 minutes; and the mixture is cooled to room temperature.

[0114] Comparative Example 8

[0115] An anti-wrinkle and whitening composition comprises, by weight, 35 parts of European plum fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0116] The preparation method of the Prunus cerasifera fruit extract is the same as that in Example 1.

[0117] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 70°C, and European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol are added, mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 35 minutes; the temperature is lowered to 45°C, and a thickener is added, and stirred for 35 minutes; and the mixture is cooled to room temperature.

[0118] Comparative Example 9

[0119] An anti-wrinkle and whitening composition comprises, by weight, 40 parts of fermentation filtrate of North American red maple leaf extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of a penetration enhancer, 0.5 parts of a thickener, 5 parts of a moisturizer, and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0120] The preparation method of the fermentation filtrate of North American red maple leaf extract is the same as that in Example 1.

[0121] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 70°C, fermentation filtrate of North American red maple leaf extract, hydroxypinacolone retinoic acid ester and tocopherol are added, mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 35 minutes; the temperature is lowered to 45°C, a thickener is added, and stirred for 35 minutes; and the mixture is cooled to room temperature.

[0122] Comparative Example 10

[0123] An anti-wrinkle and whitening composition, which comprises, by weight, 40 parts of fermentation filtrate of North American red maple leaf extract, 35 parts of European plum fruit extract, 12 parts of retinol, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0124] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0125] The preparation method of the anti-wrinkle and whitening composition is as follows: deionized water is heated to 70°C, and fermentation filtrate of North American red maple leaf extract, European plum fruit extract, retinol and tocopherol are added, and mixed evenly, and then a penetration enhancer and a moisturizer are added, and stirred for 35 minutes; the temperature is lowered to 45°C, and a thickener is added, and stirred for 35 minutes; and the mixture is cooled to room temperature.

[0126] Comparative Example 11

[0127] An anti-wrinkle and whitening composition, calculated by weight, comprises: 20 parts of fermentation filtrate of North American red maple leaf extract, 55 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0128] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0129] The preparation method of the anti-wrinkle and whitening composition is the same as that of Example 4.

[0130] Comparative Example 12

[0131] An anti-wrinkle and whitening composition, calculated by weight, comprises: 70 parts of fermentation filtrate of North American red maple leaf extract, 5 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0132] The preparation methods of the fermentation filtrate of the North American red maple leaf extract and the European plum fruit extract are the same as those in Example 1.

[0133] The preparation method of the anti-wrinkle and whitening composition is the same as that of Example 4.

[0134] Comparative Example 13

[0135] An anti-wrinkle and whitening composition, calculated by weight, comprises: 40 parts of North American red maple leaf extract, 35 parts of European plum fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of a penetration enhancer, 0.5 parts of a thickener, 5 parts of a moisturizer, and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0136] The preparation of the North American red maple leaf extract is the same as that of Comparative Example 6, and the preparation of the European plum fruit extract is the same as that of Example 1.

[0137] The preparation method of the anti-wrinkle and whitening composition is the same as that of Example 4.

[0138] Comparative Example 14

[0139] An anti-wrinkle and whitening composition, calculated by weight, comprises: 40 parts of fermentation filtrate of North American red maple leaf extract, 35 parts of European prune fruit extract, 12 parts of hydroxypinacolone retinoic acid ester, 12 parts of tocopherol, 12 parts of penetration enhancer, 0.5 parts of thickener, 5 parts of moisturizer and 90 parts of deionized water. The penetration enhancer is propylene glycol, the thickener is sodium alginate, and the moisturizer is erythritol and hyaluronic acid mixed in equal proportions.

[0140] The preparation of the fermentation filtrate of the North American red maple leaf extract was the same as that of Comparative Example 7, and the preparation of the European plum fruit extract was the same as that of Example 1.

[0141] The preparation method of the anti-wrinkle and whitening composition is the same as that of Example 4.

[0142] Test Example 1

[0143] The anti-wrinkle and whitening compositions obtained in Example 1 and Comparative Examples 1 to 7 were subjected to the following experiments:

[0144] (1) DPPH free radical scavenging experiment

[0145] DPPH was prepared into a DPPH solution with anhydrous ethanol at a concentration of 0.08mmol / L. 50μL of the composition of Example 1, the composition of Comparative Examples 1 to 7, and the solution of vitamin C (the concentration of each group was prepared with deionized water to be 0.1wt%) were added to each well of a 96-well plate. Then 150μL of the DPPH solution was added and mixed evenly. The mixture was reacted at room temperature and protected from light for 30min. The absorbance at 517nm was measured by an ELISA instrument. The group with only DPPH solution was used as a blank group. Each group was repeated 3 times, and the DPPH free radical scavenging rate was calculated and counted. The results are shown in Table 1.

[0146] DPPH free radical scavenging rate = [(A blank group - A treatment group) / A blank group] × 100%

[0147] Table 1 Statistics of DPPH free radical scavenging rate in each group

[0148]

[0149] The results showed that the DPPH free radical scavenging rate of Example 1 was significantly higher than that of the comparative examples 1 to 7 and the vitamin C group.

[0150] (2) Tyrosinase relative activity experiment

[0151] Mouse skin melanoma cells (B16-F10 cells) were cultured to the logarithmic growth phase and the cell density was adjusted to 5×10 5 100 nM α-MSH (melanin stimulator) was added, and 100 μL of the composition of Example 1, the composition of Comparative Examples 1 to 7, and the solution of vitamin C (the concentration of each group was 0.1 wt% was prepared with deionized water) were added after 24 hours, and cultured for 72 hours. The cells of each treatment group were collected separately, and then 150 μL of 1% polyethylene glycol octylphenyl ether was added to each tube, dissolved at 4°C for 30 minutes, centrifuged at 13000 r / min, 4°C for 20 minutes, and the supernatant was collected; 90 μL of cell lysate and 10 μL of 0.25% L-DOPA solution were added to each well of a 96-well plate, and the reaction was carried out at 37°C for 60 minutes. The absorbance of each well was measured at 475 nm using an enzyme marker, and the protein content of each well was determined by the BCA method, and the relative activity of tyrosinase of the cells was calculated. The control group was added with an equal amount of deionized water. Each group was repeated 3 times. The results are shown in Table 2.

[0152] Tyrosinase relative activity (%) = [(absorbance value of the drug administration group / absorbance value of the protein concentration of the drug administration group) / (absorbance value of the control group / absorbance value of the protein concentration of the control group)] × 100%

[0153] Table 2 Tyrosinase activity of cells in each group

[0154]

[0155]

[0156] The results showed that Example 1 had a significant inhibitory effect on the relative activity of tyrosinase, and the inhibitory effect was significantly higher than that of Comparative Examples 1 to 7 and the vitamin C group.

[0157] (3) Cell melanin content experiment

[0158] Mouse skin melanoma cells (B16-F10 cells) were cultured to the logarithmic growth phase and the cell density was adjusted to 5×10 5 / mL was inoculated into a 6-well plate, 2mL per well, and cultured in a 37°C, 5% CO2 incubator for 12h. After the cells adhered to the wall, the culture medium was aspirated. 2mL of 100nM α-MSH (melanin stimulator) was added, and 100μL of the composition of Example 1, the composition of Comparative Examples 1 to 7, and the solution of vitamin C were added after 24h (the concentration of each group was 0.1wt% prepared with deionized water), and cultured for 72h. 1000μL of NaOH solution (1M) containing 10% DMSO was dissolved, heated at 90°C for 2h, 100μL of cell lysis solution was aspirated, added to 96 wells, the absorbance of each well was measured at 405nm, and the protein content of each well was measured by BCA method and the relative content of cell melanin was calculated. An equal amount of deionized water was added to the control group. Each group was repeated 3 times. The results are shown in Table 3.

[0159] Relative content of melanin synthesis (%) = [(absorbance value of melanin content in the drug administration group / absorbance value of protein concentration in the drug administration group) / (absorbance value of melanin content in the control group / absorbance value of protein concentration in the control group)] × 100%

[0160] Table 3 Melanin content of cells in each group

[0161]

[0162] The results showed that the composition of Example 1 could significantly reduce the content of melanin in cells, and the effect was significantly higher than that of Comparative Examples 1 to 7 and the vitamin C group.

[0163] Test Example 2

[0164] The anti-wrinkle and whitening compositions obtained in Example 4 and Comparative Examples 8 to 14 were used to verify their effects:

[0165] The entire face of the test population (24 healthy women aged between 30 and 50, with mild fine lines on the forehead and around the eyes, and skin conditions similar to the working environment) was used as the test site. The test population was randomly divided into 8 groups, 3 people in each group, and the compositions of Example 4 and Comparative Examples 8 to 14 were applied respectively, twice a day, once in the morning and once in the evening, and the skin was cleaned before each use. No other products were used during this period. After continuous use for 3 months, the panoramic AI skin detector was used for the test population before and after use, and the scoring results before and after use were statistically analyzed. In the test results, the score of melanin metabolism was obtained by combining the anti-spot power and anti-black power, the score of the skin barrier was obtained by combining the water locking power, antibacterial power and anti-inflammatory power, the score of the spot was obtained by respectively counting the number of brown spots, ordinary spots and ultraviolet spots in the T zone and cheeks, and the score of wrinkles was obtained by combining crow's feet, wrinkles under the eyes and wrinkles on the head. The higher the score result, the better the skin condition. The scoring results of each item are shown in Table 4.

[0166] Table 4 Skin quality scores of each test group before and after use

[0167]

[0168] Taking into account the data of three people in each group, the results show that the composition of Example 4 can effectively improve the skin barrier and achieve good whitening and anti-aging effects, which is a significant improvement over the control groups 8 to 14.

[0169] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An anti-wrinkle and whitening composition, characterized in that: The invention comprises the following components by weight: 30-50 parts of fermentation filtrate of North American red maple leaf extract, 20-45 parts of European prune fruit extract, 10-18 parts of hydroxypinacolone retinoic acid ester and 10-18 parts of tocopherol.

2. The anti-wrinkle and whitening composition according to claim 1, characterized in that: The method for preparing the fermentation filtrate of the North American red maple leaf extract comprises the following steps: drying and crushing the North American red maple leaves, mixing them with water, loading them into an ultrasonic extraction kettle, and ultrasonically extracting them under airlift stirring conditions to obtain an extract; adding galacto-oligosaccharides to the extract to obtain a mixture, adding yeast to the mixture for fermentation to obtain a fermentation liquid; sterilizing and centrifuging the fermentation liquid, and taking the supernatant to obtain the fermentation filtrate of the North American red maple leaf extract.

3. The anti-wrinkle and whitening composition according to claim 2, characterized in that: The North American red maple leaf and water are mixed at a mass volume ratio of 1 g: (10-30) mL; the temperature of the ultrasonic extraction is 25-45° C., and the time of the ultrasonic extraction is 15-45 min.

4. The anti-wrinkle and whitening composition according to claim 2, characterized in that: Based on the mass of the extract, the amount of galacto-oligosaccharide added is 1-5%, and the amount of yeast added is 1×10 8 ~9×10 9 cfu / kg; the fermentation time is 10 to 30 hours, and the fermentation temperature is 30 to 40°C.

5. The anti-wrinkle and whitening composition according to claim 1, characterized in that: The preparation method of the Prunus cerasifera fruit extract comprises the following steps: crushing the Prunus cerasifera fruit, mixing with ethanol, heating and refluxing for extraction to obtain the Prunus cerasifera fruit extract, filtering, concentrating and freeze-drying the Prunus cerasifera fruit extract to obtain the Prunus cerasifera fruit extract.

6. The anti-wrinkle and whitening composition according to claim 5, characterized in that: The European holly fruit and ethanol are mixed according to 10g: (100-120)mL; the heating reflux extraction time is 4-6h.

7. The anti-wrinkle and whitening composition according to claim 1, characterized in that: The invention also includes the following components by weight: 10 to 15 parts of a penetration enhancer, 0.2 to 0.8 parts of a thickener, 3 to 9 parts of a moisturizer and 80 to 100 parts of deionized water.

8. The method for preparing the anti-wrinkle and whitening composition according to claim 7, characterized in that: The following steps are involved: Heat deionized water to 60-80°C, add fermentation filtrate of North American red maple leaf extract, European plum fruit extract, hydroxypinacolone retinoic acid ester and tocopherol, mix well, then add penetration enhancer and moisturizer, stir for 30-50 minutes; cool to 40-50°C, add thickener, stir for 30-50 minutes; cool to room temperature.

9. Use of the anti-wrinkle and whitening composition according to any one of claims 1 to 7 in the preparation of anti-wrinkle and / or whitening products, characterized in that: The products include skin care products, cosmetics, and cleaning products.

10. An anti-wrinkle and / or whitening product, characterized in that: The product comprises the anti-wrinkle and whitening composition according to any one of claims 1 to 7, wherein the content of the anti-wrinkle and whitening composition is 10 to 100 wt %.

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