Bionic vaginal fluid with moisturizing and anti-inflammatory effects as well as preparation method and application of bionic vaginal fluid

Abstract

The invention belongs to the technical field of female and amphoteric external product raw materials, and particularly relates to bionic vaginal fluid with moisturizing and anti-inflammatory effects as well as a preparation method and application of the bionic vaginal fluid. The bionic vaginal fluid is prepared from the following components in parts by weight: 0.1 to 2 parts of scale microspora polysaccharide and 0.1 to 1 part of succinyl glycan. The bionic vaginal fluid is prepared by innovatively compounding the microspora squara polysaccharide and the succinyl glycan, the two components are matched and interacted with each other and can play a synergistic effect, so that the moisture retention and the anti-inflammatory soothing effect of the bionic vaginal fluid are remarkably improved, a lubricating feeling and a very good moistening feeling can be provided for a vagina, and the vaginal fluid has a good anti-inflammatory effect and a good anti-inflammatory effect. Meanwhile, micro-ecological balance in the vagina is maintained, and the immunity of the vagina is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of raw materials of external products for women and both sexes, and in particular relates to a bionic vaginal fluid with moisturizing and anti-inflammatory effects, and a preparation method and application thereof. Background Art

[0002] Existing bionic vaginal fluid or other bionic body fluid products mainly imitate the components of vaginal secretions, and add other corresponding components such as bactericides and thickeners to achieve corresponding purposes and effects. Owen DH and Katz DF provided a specific formula of a vaginal secretion fluid simulant: NaCl 3.51%; KOH 1.40%; Ca(OH)2 0.222%; bovine serum albumin 0.018%; lactic acid 2.00%; acetic acid 1.00%; glycerol 0.16%; urea 0.4%; glucose 5.0%. This developed liquid is based on the existing analysis of the components of human vaginal secretions, thereby simulating human vaginal fluid. CN103126917B discloses a bionic gynecological care liquid and its preparation method. In this patent, artificial body fluid is used as a solvent, and antimicrobial peptides are selected as bactericides to obtain artificial vaginal secretions that can treat vaginitis.

[0003] However, the existing bionic vaginal fluid or other bionic body fluids simply imitate vaginal fluid and have a weak moisturizing effect. In addition, some bionic vaginal fluids need to add other ingredients such as bactericides and thickeners to achieve specific effects, which may not only destroy the balance of vaginal microecology, but also cause damage to the vaginal mucosa after long-term repeated use, thereby affecting vaginal health and causing vaginal dryness and other problems. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a bionic vaginal fluid with moisturizing and anti-inflammatory effects and its preparation method and application, so as to exert the shrinking, moisturizing, soothing and anti-inflammatory effects, thereby providing lubrication and good moisturizing to the vagina, helping to maintain the microecological balance in the vagina and improve the immune resistance of the vagina.

[0005] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0006] In a first aspect, the present invention provides a bionic vaginal fluid with moisturizing and anti-inflammatory effects, wherein the bionic vaginal fluid comprises the following components in parts by weight: 0.1-2 parts of microsporum polysaccharide and 0.1-1 parts of succinoglycan.

[0007] The first line of defense of the human body is composed of skin and mucous membranes and their secretions, which can block most pathogens from invading the human body. The main component of the mucosal barrier is mucin, and the basic structure of mucin is glycoprotein, and the vaginal mucosa itself also secretes a mucin-rich liquid as a lubricant. The microsporum polysaccharide used in the present invention is extracted from the nameko mushroom, which is a mucus barrier component secreted by plants. It is a unique polysaccharide similar to the biological mucosal protective component mucin. This biological molecule composed of protein and sugar can protect the mucosal cell layer in contact with the external air layer from drying and external stimulation, and has both moisturizing and barrier functions; at the same time, as a polysaccharide, it has the effect of prebiotics and can regulate the vaginal microecology; in addition, the microsporum polysaccharide has a sticky and slippery moisturizing effect, and its water retention effect is 6 times that of hyaluronic acid, with an extremely high water retention rate, not only good moisturizing effect, but also excellent anti-inflammatory and soothing effect.

[0008] Succinoglycan is an extracellular active polysaccharide composed of glucose and galactose produced by fermentation. It also has a moisturizing effect. At the same time, it can reduce the response of inflammatory factors, enhance the immune activity of epidermal cells, and thus enhance the resistance of the skin barrier.

[0009] Experimental research has found that the present invention innovatively uses a compound of microsporum polysaccharides and succinoglycan to prepare bionic vaginal fluid. The two components cooperate with each other and interact with each other to exert synergistic enhancement to significantly improve the moisturizing and anti-inflammatory soothing effects of the bionic vaginal fluid. It will not excessively change the vaginal microenvironment or destroy the physiological balance of the vagina, and can be used for daily vaginal care.

[0010] Moreover, when the inventors explored the dosage ratio of the two components, they found that when the ratio of microsporum polysaccharide and succinoglycan exceeded the above preferred range, the synergistic effect between the components was not obvious or even produced an antagonistic effect, which led to a significant decrease in the moisturizing and anti-inflammatory effects of the bionic vaginal fluid, indicating that the above two components can only play an ideal synergistic role within the specified range of the present invention.

[0011] Preferably, the bionic vaginal fluid comprises the following components in parts by weight: 0.5-1 part of microsporum polysaccharide and 0.1-0.5 part of succinoglycan.

[0012] Preferably, the bionic vaginal fluid comprises the following components in parts by weight: 0.6 parts of microsporum polysaccharide and 0.4 parts of succinoglycan.

[0013] Experimental research has found that when Microsporum polysaccharide and succinoglycan are compounded in the above-mentioned optimal dosage ratio, the synergistic effect of the two is the best, thereby maximizing the moisturizing, shrinking, soothing and anti-inflammatory effects of the bionic vaginal fluid, and effectively maintaining the microecological balance in the vagina, thereby improving the immune resistance of the vagina.

[0014] Preferably, the mass concentration of the Lepidolophus microsporus polysaccharide is 0.5%, and the mass concentration of the succinoglycan is 1%.

[0015] Preferably, based on 100 parts by weight, the bionic vaginal fluid further comprises the following components in parts by weight: 4-6 parts of preservatives, 0.6-1.5 parts of moisturizers, 1-3 parts of lactic acid, 0.05-0.15 parts of pH regulators and the balance of water.

[0016] Preferably, based on 100 parts by weight, the bionic vaginal fluid further comprises the following components in parts by weight: 4 parts of preservative, 1.1 parts of moisturizer, 2 parts of lactic acid, 0.1 parts of pH regulator and the balance of water.

[0017] Preferably, the preservative comprises 1,2-hexanediol, raspberry ketone and butanediol, and the mass ratio of 1,2-hexanediol, raspberry ketone and butanediol is 0.6:0.4:(3-5).

[0018] More preferably, the mass ratio of 1,2-hexanediol, raspberry ketone and butanediol is 0.6:0.4:3.

[0019] Preferably, the moisturizing agent comprises glycerol, urea and allantoin, and the mass ratio of the glycerol, urea and allantoin is (0.2-0.5):(0.2-0.5):(0.2-0.5).

[0020] More preferably, the mass ratio of glycerol, urea and allantoin is 0.4:0.4:0.3.

[0021] In the preparation of the bionic vaginal fluid of the present invention, glycerol, urea and allantoin are added as moisturizing ingredients. The synergistic effect of the three can further enhance the moisturizing effect of the bionic vaginal fluid, and can improve the comfort of the vaginal mucosa to a certain extent, provide additional repair and protection effects, thereby helping the bionic vaginal fluid to achieve better care effects.

[0022] Preferably, the pH adjuster comprises potassium hydroxide.

[0023] In a second aspect, the present invention provides the use of the bionic vaginal fluid in the preparation of female or bisexual intimate products.

[0024] Preferably, the bionic vaginal fluid is used as a raw material for external use products, and its mass percentage in female or bisexual intimate products is 5-10%.

[0025] The anti-bacterial vaginal fluid of the present invention can be applied to the fields of cosmetics, disinfectants, and is particularly suitable for use in female and bisexual intimate products. When it is diluted and added at 5.0-10%, it can be similar to the composition, viscosity, and pH of human vaginal secretions. It can not only play a moisturizing and anti-inflammatory role, but also enhance the comfort and health of the vaginal area and help maintain the natural barrier function of the vagina.

[0026] In a third aspect, the present invention provides a method for preparing the bionic vaginal fluid, comprising the following steps:

[0027] S1. Heat and stir the preservative at 65-75° C. to obtain a preservative system;

[0028] S2, adding moisturizer, microsporum polysaccharide, succinoglycan and water to the preservative system, and stirring until the system is clear and transparent;

[0029] S3, adding lactic acid, and adjusting the pH of the system to 4.0-4.5 with a pH regulator, thereby obtaining the bionic vaginal fluid.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention innovatively adopts a compound of microsporum polysaccharide and succinoglycan to prepare bionic vaginal fluid. The two components cooperate with each other and interact with each other to exert synergistic enhancement to significantly improve the moisturizing and anti-inflammatory soothing effects of the bionic vaginal fluid, thereby providing lubrication and good moisturizing to the vagina, helping to maintain the microecological balance in the vagina and improving the immune resistance of the vagina. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a result diagram of the effect of the bionic vaginal fluid on the OD value of Lactobacillus crispatus described in Effect Example 3;

[0033] Figure 2 This is a graph showing the effect of the bionic vaginal fluid on the pH value of Lactobacillus crispatus described in Effect Example 3. DETAILED DESCRIPTION

[0034] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0035] Unless otherwise specified, all raw materials involved in the following examples and comparative examples are commercially available general raw materials.

[0036] The polysaccharide of Microspora was purchased from Shanghai Yucui Chemical Technology Co., Ltd., and the succinoglycan was purchased from Shanghai Gefeng Biotechnology Co., Ltd.; in the examples and comparative examples, the polysaccharide of Microspora was a polysaccharide solution with a mass concentration of 0.5%, and the succinoglycan was a succinoglycan solution with a mass concentration of 1%.

[0037] Examples 1-5

[0038] This embodiment 1-5 provides a bionic vaginal fluid with moisturizing and anti-inflammatory effects, and the specific formula is shown in Table 1 (total weight parts is 100 parts). The preparation method of the bionic vaginal fluid comprises the following steps:

[0039] S1. Heat and stir the preservative at 65-75° C. to obtain a preservative system;

[0040] S2, adding moisturizer, microsporum polysaccharide, succinoglycan and water to the preservative system, and stirring until the system is clear and transparent;

[0041] S3, adding lactic acid, and adjusting the pH of the system to 4.0-4.5 with a pH regulator, thereby obtaining the bionic vaginal fluid.

[0042] Table 1 Formulation of the biomimetic vaginal fluid described in Examples 1-5 (parts by weight)

[0043]

[0044]

[0045] Comparative Examples 1-5

[0046] The present invention also provides the formula of the bionic vaginal fluid of comparative examples 1-5, as shown in Table 2 (total weight parts is 100 parts). The preparation method of the bionic vaginal fluid provided in comparative examples 1-5 is similar to that of embodiments 1-5, except that the formula is different.

[0047] Table 2 Formula of the bionic vaginal fluid described in Comparative Examples 1-5 (parts by weight)

[0048]

[0049]

[0050] Comparative Example 6

[0051] This comparative example provides a bionic vaginal fluid, which is different from Example 1 only in that hyaluronic acid is used instead of microsporum polysaccharide, and the other components and amounts are the same as those in Example 1.

[0052] Comparative Example 7

[0053] This comparative example provides a bionic vaginal fluid, which is different from Example 1 only in that β-glucan is used instead of succinoglycan, and the other components and amounts are the same as those in Example 1.

[0054] Effect Example 1

[0055] This effect example uses the bionic vaginal fluid (5% water dilution) prepared in Examples 1-5 and Comparative Examples 1-7 as samples to measure its moisture retention, and the specific method is as follows:

[0056] (1) Select a product test area and a blank control area on the forearm. Use a skin moisture tester (Corneometer) to measure the corneum moisture content Corneometer basic value;

[0057] (2) Test method: The flexed side of the forearm is used as the test area, and the test area is 3cm*3cm. No product is used in the blank control area, and the test sample is evenly applied once on the product test area (2±0.1)μL / cm 2 , start timing after application. Use a skin moisture tester (Corneometer) to measure the keratin moisture content 30 minutes after application; test conditions: temperature 21℃±1℃, humidity 50%RH±5%RH;

[0058] (3) The keratin moisture content and moisture gain were calculated respectively. Each group of samples was repeated three times and the final result was averaged. The results are shown in Table 3.

[0059] Table 3 Difference in water content of skin stratum corneum

[0060]

[0061]

[0062] It can be seen from the results in Table 1 that the bionic vaginal fluid prepared by the technical solution of the present invention has a good moisturizing effect, thereby providing a better lubrication and moisturizing feeling for the vagina. In particular, the bionic vaginal fluid prepared in Example 1 significantly increased the moisture content of the skin stratum corneum by 75.69% after testing, and had the best moisturizing effect.

[0063] When the microsporum polysaccharide and succinoglycan components are respectively lacking in comparative examples 1-2, the moisturizing effect of the prepared biomimetic vaginal fluid is significantly reduced compared with the embodiment. When the dosage ratio of the two components in comparative examples 4-5 exceeds the preferred range of the present invention, the moisturizing effect of the prepared biomimetic vaginal fluid is also reduced, indicating that the compound of the microsporum polysaccharide and succinoglycan described in the present invention can synergistically enhance the moisturizing effect of the biomimetic vaginal fluid, and the best synergistic effect can be exerted when the dosage of the two is within the specified range of the present invention; and when the two components are replaced by other components with similar effects in comparative examples 6-7, the moisturizing and water-locking effect of the prepared biomimetic vaginal fluid is also significantly reduced, that is, the synergistic effect is not obvious. Therefore, the microsporum polysaccharide and succinoglycan components in the biomimetic vaginal fluid described in the present invention cannot be replaced by conventional components.

[0064] Effect Example 2

[0065] This effect example uses the bionic vaginal fluid (5% water dilution) prepared in Examples 1-5 and Comparative Examples 1-7 as samples to measure their anti-inflammatory and soothing effects.

[0066] The samples were tested according to the laboratory method HMC-WI-029 "Hyaluronidase Inhibition Rate". When the hyaluronidase inhibition rate of the positive control (1% dipotassium glycyrrhizinate) was >50%, the reaction system was considered effective; when the hyaluronidase inhibition rate of the sample was higher than that of the negative control (distilled water) and there was a significant difference (P<0.05), the test sample was considered to have a certain soothing effect. The specific test results are shown in Table 4.

[0067] Table 4 Hyaluronidase inhibition rate

[0068]

[0069]

[0070] The hyaluronidase inhibition rate of the sample was higher than that of the negative control and the difference was significant (P<0.05). It can be considered that the test sample has a certain soothing effect; *** indicates that the sample or positive control is significantly different from the negative control (P<0.001); ** indicates that the sample or positive control is significantly different from the negative control (P<0.01).

[0071] As shown in Table 4, the bionic vaginal fluids described in Examples 1-5 have certain soothing and anti-inflammatory effects, thereby effectively improving the comfort of the vaginal mucosa, reducing discomfort or rejection caused by the intervention of foreign substances, and providing a more gentle nursing effect. However, in Comparative Examples 1-7, due to the lack of microsporum polysaccharide and succinoglycan components or the ratio of the two exceeding the preferred range of the present invention, the soothing and anti-inflammatory effects of the prepared bionic vaginal fluids are significantly reduced compared with the examples.

[0072] Effect Example 3

[0073] This effect example uses the bionic vaginal fluid prepared in Example 1 as a sample, and tests the proliferation of Lactobacillus crispatus at different addition concentrations (5% and 8%). The specific method is as follows:

[0074] In conventional MRS culture medium, 5% and 8% of the biomimetic vaginal fluid described in Example 1 are used to replace glucose, respectively, to prepare experimental culture medium, in addition, the basic culture medium without carbon source is used as the blank control group. The pH value of all the above culture mediums is adjusted to 6.2±0.2, and sterilized in a 121°C autoclave for 20min for stand-by use after packaging. Take an appropriate amount of Lactobacillus crispatus freeze-dried powder, add sterile distilled water to dissolve, apply a plate, and culture for 24 hours under 37°C. Select a single colony, inoculate it in liquid MRS culture medium, culture at 37°C for 16h, and inoculate the activated strain into the experimental MRS culture medium at an inoculum size of 1%, culture at 37°C for 48 hours, and measure the OD value and pH value. The OD value is the absorbance value measured at 600nm by an ultraviolet spectrophotometer. The OD value can indicate the amount of Lactobacillus crispatus, i.e., the proliferation of Lactobacillus crispatus, and the OD value rises; the large-scale reproduction of Lactobacillus crispatus will produce more extracellular secretions, i.e., lactic acid. When the lactic acid increases, the pH value of the system decreases. The specific test results are shown in Table 5.

[0075] Table 5

[0076] Sample source OD value after 48h of culture pH value after 48h of culture Blank control group 0.1999 5.61 5% Bionic Vaginal Fluid 0.3416 4.62 8% Bionic Vaginal Fluid 0.3951 4.55

[0077] Lactobacillus crispatus is one of the common probiotics in the vagina and has important probiotic effects, especially playing a key role in the health of the female vagina. Figure 1-2 The results show that the bionic vaginal fluid of the present invention can effectively promote the proliferation of Lactobacillus crispatus, thereby helping to maintain and restore the microecological balance of the vagina, enhance immune defense, prevent and alleviate various vaginal infections and inflammatory reactions, and effectively maintain vaginal health.

[0078] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A bionic vaginal fluid with moisturizing and anti-inflammatory effects, characterized in that: The bionic vaginal fluid comprises the following components in parts by weight: 0.1-2 parts of microsporum polysaccharide and 0.1-1 parts of succinoglycan.

2. The bionic vaginal fluid according to claim 1, characterized in that: The bionic vaginal fluid comprises the following components in parts by weight: 0.5-1 part of microsporum polysaccharide and 0.1-0.5 part of succinoglycan.

3. The bionic vaginal fluid according to claim 2, characterized in that: The bionic vaginal fluid comprises the following components in parts by weight: 0.6 parts of microsporum polysaccharide and 0.4 parts of succinoglycan.

4. The bionic vaginal fluid according to any one of claims 1 to 3, characterized in that: Based on 100 parts by weight, the bionic vaginal fluid further comprises the following components in parts by weight: 4-6 parts of preservatives, 0.6-1.5 parts of moisturizers, 1-3 parts of lactic acid, 0.05-0.15 parts of pH regulators and the balance of water.

5. The bionic vaginal fluid according to claim 4, characterized in that: Based on 100 parts by weight, the bionic vaginal fluid further comprises the following components in parts by weight: 4 parts of preservative, 1.1 parts of moisturizer, 2 parts of lactic acid, 0.1 parts of pH regulator and the balance of water.

6. The bionic vaginal fluid according to claim 4 or 5, characterized in that: The preservative comprises 1,2-hexanediol, raspberry ketone and butanediol, and the mass ratio of the 1,2-hexanediol, raspberry ketone and butanediol is 0.6:0.4:(3-5).

7. The bionic vaginal fluid according to claim 4 or 5, characterized in that: The moisturizing agent comprises glycerin, urea and allantoin, and the mass ratio of the glycerin, urea and allantoin is (0.2-0.5):(0.2-0.5):(0.2-0.5).

8. Use of the bionic vaginal fluid according to any one of claims 1 to 7 in the preparation of female or bisexual intimate products.

9. The use according to claim 8, characterized in that The mass percentage of the bionic vaginal fluid in female or bisexual intimate products is 5-10%.

10. The method for preparing the bionic vaginal fluid according to any one of claims 4 to 7, characterized in that: The following steps are involved: S1. Heat and stir the preservative at 65-75° C. to obtain a preservative system; S2, adding moisturizer, microsporum polysaccharide, succinoglycan and water to the preservative system, and stirring until the system is clear and transparent; S3, adding lactic acid, and adjusting the pH of the system to 4.0-4.5 with a pH regulator, thereby obtaining the bionic vaginal fluid.

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