Application of plant lactobacillus LZU-LJY-1Q1 in preparation of product for improving memory ability damage

Abstract

The invention provides application of plant lactobacillus LZU-LJY-1Q1 in preparation of a product for improving memory ability damage, and belongs to the technical field of microorganisms. The invention finds that the plant lactobacillus LZU-LJY-1Q1 can achieve the effect of improving memory ability damage by improving neuron damage, resisting inflammation, improving oxidative stress and balancing brain cholinergic nervous system activity, and meanwhile, the plant lactobacillus LZU-LJY-1Q1 can be planted in human intestinal tracts and reproductive systems, so that host microecological balance is improved, beneficial effects on the intestinal tracts are achieved, and the effect of improving memory ability damage is achieved. And the effect of preventing memory impairment is achieved by reducing intestinal inflammatory response and adjusting the intestinal brain axis.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and in particular relates to application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing a product for improving impaired memory. Background Art

[0002] Impaired memory is mainly caused by physiological aging, psychological factors, malnutrition and brain diseases such as Alzheimer's disease, and its pathogenesis is complex. Since cholinergic nerves in the nervous system constitute an important biological basis for learning and memory, it is now believed that impaired function of the cholinergic system in the brain is an important factor leading to memory impairment. However, existing studies have revealed the key role of oxidative stress and neuroinflammation, which may lead to interruption of synaptic transmission and induce apoptosis of nerve cells including cholinergic neurons, thereby exacerbating learning and cognitive impairment in the process of memory impairment. Chronic intestinal inflammation can lead to systemic inflammatory responses, damage the blood-brain barrier, stimulate neuroinflammatory processes, and ultimately exacerbate the occurrence of neurodegenerative diseases.

[0003] At present, Lactobacillus plantarum has been widely used in nutrition and health care, fermented food, biomedicine, feed, animal husbandry and other industries. It has good anti-inflammatory and antioxidant effects, can improve the intestinal environment, and regulating the gut-brain axis may be a good way to prevent memory impairment, but how to screen a Lactobacillus plantarum that can regulate the intestine to achieve better improvement of genetic damage still needs to be studied. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a use of Lactobacillus plantarum LZU-LJY-1Q1 in the preparation of a product for improving impaired memory.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing medicines for improving impaired memory. The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0007] Preferably, the Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory by balancing the cholinergic system.

[0008] Preferably, the Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory ability by reducing neuroinflammation.

[0009] Preferably, the Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory ability by reducing oxidative stress.

[0010] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food for assisting in improving memory. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0011] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food which is helpful for regulating intestinal flora. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0012] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food helpful for anti-oxidation. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0013] Preferably, the food comprises health food.

[0014] Preferably, the method of using the Lactobacillus plantarum LZU-LJY-1Q1 comprises preparing the Lactobacillus plantarum LZU-LJY-1Q1 into a bacterial suspension;

[0015] The number of viable bacteria in the bacterial suspension is ≥ 1×10 7 CFU / ml.

[0016] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing cosmetics. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0017] Preferably, the method of using the Lactobacillus plantarum LZU-LJY-1Q1 comprises preparing the Lactobacillus plantarum LZU-LJY-1Q1 into a bacterial suspension;

[0018] The number of viable bacteria in the bacterial suspension is ≥ 1×10 7 CFU / ml.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention finds that Lactobacillus plantarum LZU-LJY-1Q1 can improve impaired memory by improving neuronal damage, anti-inflammation, improving oxidative stress and balancing the activity of the brain cholinergic nervous system. At the same time, it can be colonized in the human intestine and reproductive system, thereby improving the host microecological balance and exerting a beneficial effect on the intestine. It can also reduce intestinal inflammatory response and regulate the gut-brain axis to achieve the effect of preventing memory damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1is a growth curve diagram of Lactobacillus plantarum LZU-LJY-1Q1;

[0022] Figure 2 This is the result of Gram staining observed under a microscope;

[0023] Figure 3 The results of the water maze test are shown in Figure 1, where A is the escape latency of mice, B is the time taken by mice to reach the target quadrant, and C is the frequency of mice passing through the target quadrant;

[0024] Figure 4 This is a test diagram of the short-term non-spatial recognition memory behavior of mice, where A is the new object recognition index of mice, and B is the preference index of mice;

[0025] Figure 5 The figure is the pathological changes of neurological tissues in mice;

[0026] Figure 6 This is a staining image of mouse colon tissue;

[0027] Figure 7 is the content of inflammatory factors in mice, where A is interleukin 1β (IL-1β), B is tumor necrosis factor-α (TNF-α), and C is interleukin-6 (IL-6);

[0028] Figure 8 Figure 1 is a graph of SCOP-induced oxidative stress in mice, where A is the total superoxide dismutase (SOD) content in serum, B is the SOD content in hippocampal tissue, C is the MDA content in hippocampal tissue, D is the reduced glutathione (GSH) content in hippocampal tissue, and E is the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSH) in hippocampal tissue.

[0029] Fig. 9 The content of acetylcholine (AcH) and acetylcholinesterase (AChE) in mouse hippocampal tissue, where A is the AcH content and B is the AChE content.

[0030] Biological Deposit Description

[0031] Lactiplantibacillus plantarum LZU-LJY-1Q1 was deposited in the Guangdong Provincial Microbiological Culture Collection Center on July 1, 2024. The deposit address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, and the deposit number is GDMCC NO: 64797. DETAILED DESCRIPTION

[0032] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing medicines for improving impaired memory. The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0033] In the present invention, the Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory by balancing the cholinergic system, reducing neuroinflammation, and reducing oxidative stress.

[0034] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food for assisting in improving memory. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797. The Lactobacillus plantarum LZU-LJY-1Q1 assists in improving memory by balancing the cholinergic system, reducing neuroinflammation and reducing oxidative stress.

[0035] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food which is helpful for regulating intestinal flora. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0036] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food helpful for anti-oxidation. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0037] In the present invention, the food preferably includes health food.

[0038] In the present invention, the Lactobacillus plantarum LZU-LJY-1Q1 is preferably used in the form of a bacterial suspension of Lactobacillus plantarum LZU-LJY-1Q1;

[0039] The number of live bacteria in the bacterial suspension is preferably ≥ 1×10 7 CFU / ml, preferably ≥5×10 7 CFU / ml, preferably ≥1×10 8 CFU / ml.

[0040] The invention provides application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing cosmetics. The preservation number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO: 64797.

[0041] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0042] Sources:

[0043] Scopolamine hydrobromide was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0044] Acetylcholine (ACH) assay kit, acetylcholinesterase (A-CHE) assay kit, malondialdehyde (MDA) assay kit, total superoxide dismutase (T-SOD) assay kit, interleukin-1β (IL-1β) assay kit, tumor necrosis factor-α (TNF-α) assay kit, reduced glutathione (GSH) assay kit, and oxidized glutathione (GSSH) assay kit were purchased from Nanjing Jiancheng Bioengineering Institute;

[0045] The Gram staining solution kit was purchased from Beijing Luqiao Technology Co., Ltd.;

[0046] BALB / c male mice were purchased from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;

[0047] Experimental Example 1

[0048] (1) Strain culture:

[0049] Lactobacillus plantarum LZU-LJY-1Q1 was spread on MRS agar plates, and a single colony was transferred to 8 mL MRS broth medium. After incubation at 37 °C for 20 h, a first-generation bacterial solution was obtained. Then 200 μL of the bacterial solution was transferred to 9.8 mL MRS broth medium. After incubation at 37 °C for 18 h, a concentration of 1 × 10 8 CFU / mL strain, obtain bacterial solution, and store at 4℃ before use.

[0050] (2) Growth curve of strains

[0051] 200 μL of bacterial solution was inoculated into 9.8 mL of MRS broth medium and cultured at 37°C for 0, 6, 12, 18, 24 and 30 h. The OD600 was monitored. The results were as follows: Figure 1 shown.

[0052] (3) Identification characteristics

[0053] (3.1) Gram staining experiment

[0054] Use Gram staining kit to stain the strain. Observe Gram staining results under microscope. Figure 2 , purple in color, and short rod-shaped in cell morphology, so strain LZU-LJY-1Q1 is a Gram-positive bacterium.

[0055] (3.2) Identification and preservation of strain LZU-LZY-1Q1

[0056] The sequencing results were provided by Guangdong Meige Gene Technology Co., Ltd. The results of the 16S rRNA sequence analysis of strain LZU-LZY-1Q1 are as follows: The strain LZU-LZY-1Q1 was determined to be a strain of Lactobacillus plantarum.

[0057] Sanger sequencing

[0058] Sequencing results:

[0059] SEQ ID NO.1:>LZU-LZY-1Q1.8F_Clean seq.

[0060] ACGAACTCTGGTATTGATTGGTGCTTGCATCATGATTTACATTTGAGTGAGTGGCGAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCGGGGGATAACACCTGGAAACAGATGCTAATACCGCATAACAACTTGGACCGCATGGTCCGAGCTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTAGATGGTGGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTGACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTCAACCGAAGAAGTGCATCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATTAGATAC CCTGGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGT

[0061] SEQ ID NO.2:>LZU-LZY-1Q1.16SR_Clean seq.

[0062] AAAGGTTACCCCACCGACTTTGGGTGTTACAAACTCTCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGACTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGCTTTAAGAGATTAGCTTACTCTCGCGAGTTCGCAACTCGTTGTACCATCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCACCAGAGTGCCCAACTTAATGCTGGCAACTGATAATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAATCTCTTAGATTTGCATAGTATGTCAAGACCTGGTAAGGTTCTTCGCGTAGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGCCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCAGCACTGAAGGGCGGAAACCCTCCAACACTTAGCATTCATCGTTTACGGTATGGACTACCAGGGTATCTAATCCTGTTTGCTACCCATACTTTCGAGCCTCAGCGTCAGTTACAGACCAGACAGCCGCCTTCGCCACTGGTGTTCTTCCATATATCTACGCATTTCACCGCTACACATGGAGTTCCACTGTCCTCTTCTGCACTCA

[0063] (3.2.1) Sequence splicing

[0064] The sequence splicing was performed using the mothur software. The splicing results of LZU-LZY-1Q1 are shown in Table 1 below.

[0065] Table 1 Splicing results of LZU-LZY-1Q1

[0066] Length OverlapLength OverlapStart OverlapEnd MisMatches NumNs ExpectedErrors 1411 189 611 800 0 0 1.50004

[0067] (3.2.2) Species annotation

[0068] The spliced ​​sequences were blasted against the NCBI nt library to obtain species annotation and classification results.

[0069] Table 2 LZU-LZY-1Q1 annotation classification results

[0070]

[0071] As shown in Table 2, the strain belongs to the genus Lactobacillus (Lactobacillales), and it was preliminarily determined that the strain was Lactobacillus plantarum LZU-LJY-1Q1.

[0072] Experimental Example 2

[0073] (1) Experimental animals

[0074] This study was conducted in accordance with the Gansu Provincial Laboratory Animal Management Standards and the China Laboratory Animal Management Standards. All experimental procedures and techniques were reviewed and approved by the Animal Ethics Committee of Lanzhou University. 40 BALB / c male mice (9-10 weeks old, weighing 18-22 g) were placed in an animal facility that meets specific pathogen-free (SPF) requirements (room temperature 20-22 °C, humidity 45-55%, 12 h light / dark cycle) with unrestricted access to standard laboratory diet and water.

[0075] 4.2 Memory impairment modeling and experimental procedures

[0076] 40 BALB / c mice were randomly placed in 8 cages and adaptively raised for 7 days. After 7 days, the mice were randomly divided into 4 groups, 10 in each group, namely blank control group (group C), model group (group M), positive control donepezil hydrochloride tablets group (group P) and Lactobacillus plantarum LZU-LJY-1Q1 group (group L). From the 21st day to the 29th day, the Morris water maze test and novel object recognition test were performed in groups M, P and L 30 minutes after SCOP injection, and the mice were dissected after completion.

[0077] The specific experimental groups include:

[0078] The blank control group, referred to as “Group C,” was gavaged with 200 μL of sterile water daily for 29 days;

[0079] The model group, referred to as "M group", was gavaged with 200 μL sterile water every day for 29 days; SCOP (3 mg / kg) was injected intraperitoneally every day from 21st day to 29th day.

[0080] The positive control group of donepezil hydrochloride tablets, referred to as "Group P", was gavaged with 200 μL of donepezil hydrochloride (3 mg / kg) every day for 29 days; scopolamine hydrobromide (SCOP) (3 mg / kg) was intraperitoneally injected every day from the 21st day to the 29th day.

[0081] The Lactobacillus plantarum LZU-LJY-1Q1 group, referred to as the "L group", was gavaged with 200 μL of sterilized bacterial solution every day for 29 days; and SCOP (3 mg / kg) was intraperitoneally injected every day from the 21st day to the 29th day. The preparation method of the sterilized bacterial solution is as follows: 6 mL of the bacterial solution prepared in step (1) of Experimental Example 1 was added to 200 mL of LMRs broth medium, cultured at 37°C for 18 hours, centrifuged (4°C, 6000 r / min, 10 minutes), the supernatant was taken, concentrated by half, sterilized at 90°C for 1 hour, and placed in a 4°C refrigerator for standby use.

[0082] 4.3 Morris water maze test

[0083] The water maze test was used to observe the effects on the spatial memory and learning ability of mice. First, the mice underwent habituation training before the test, during which food-grade leucomelanin was added to the swimming pool to form a color difference for instrument monitoring. The mice were released from each quadrant in turn on days 1, 2, 3, 4, and 5. The time it takes for the mouse to reach the platform during the experiment was recorded. If the mouse failed to find the quadrant platform within 60 seconds, it was guided to the platform with a stick and stayed there for 30 seconds, and the escape latency would be recorded as 60 seconds. On day 6, the transparent platform was removed and the animal was placed in the pool for 60 seconds. The time the mouse spent in the target quadrant of the pool and the number of times it crossed the target platform were recorded and analyzed. See the results. Figure 3 On the fifth day of the mice's navigation, the L group reduced the mice's escape latency on day 5. In the spatial exploration test, the L group increased the SCOP-induced mice's stay time in the target quadrant and the number of times they crossed the target platform, improving the mice's memory.

[0084] 4.4 New Object Recognition Test

[0085] Behavioral tests to evaluate the short-term non-spatial recognition memory of experimental animals. In the familiarization phase (first day), two identical objects were placed in the test box; then, the mice were placed at the same distance from the two objects and allowed to explore freely for 6 min. In the test phase (second day), one of the objects was replaced by a new object, and the time the mice spent on the novel object (new object) and the familiar object (old) was recorded. The discrimination index (DI) and preference index (PI) were used to evaluate the short-term non-spatial recognition memory of experimental mice. PI = N / (N+F); DI = (NF) / (N+F), where N represents the exploration time of the mouse on the new object, and F represents the exploration time of the mouse on the familiar object.

[0086] The results are as follows Figure 4 As shown, compared with group C, group M had lower novel object recognition index and preference index; while group L had higher novel object recognition index and preference index compared with group M, with the indexes increased by 70.94% and 75.98% respectively. In the novel object recognition test, group L increased the novel object recognition index and preference index of SCOP-induced mice and improved the memory of mice.

[0087] 4.5 Hematoxylin-eosin (HE) staining of hippocampal and colonic tissues

[0088] The dissected mouse whole brain tissue was placed in a 4% paraformaldehyde solution. The mouse body was placed on ice and then rapidly dissected to obtain colon samples, which were washed with phosphate buffered saline (PBS, pH 7.2) to remove residual intestinal contents and fixed with 4% paraformaldehyde.

[0089] To determine whether Lactobacillus plantarum LZU-LJY-1Q1 can protect neurons from SCOP-induced neuronal damage, HE staining was used to detect histopathological changes. Figure 5 As shown, the hippocampal tissue texture of SCOP-induced mice was disordered, the hippocampal cells were sparsely and scattered, the number was reduced, the cells were deeply stained, and the color was darker, resulting in nuclear condensation and neuronal damage. Lactobacillus plantarum LZU-LJY-1Q1 prevented and treated SCOP-induced memory impairment mice, and the results showed that the proportion of abnormal cells decreased and there was a certain improvement in tissue morphology. The experimental results show that Lactobacillus plantarum LZU-LJY-1Q1 can effectively improve SCOP-induced neuronal damage in mice.

[0090] In order to explore the protective effect of Lactobacillus plantarum LZU-LJY-1Q1 on the colon, the colon tissue was stained with HE staining and histological analysis was performed. Figure 6 The colon pathological morphology of group C was normal, the mucosa was intact, and there was no inflammatory infiltration. The crypts of group M were damaged, most of the goblet cells were exhausted, and the inflammatory infiltration was obvious; the damage to the integrity of the colon tissue was improved in group L, and the colon pathology was improved to a certain extent. The results showed that the colon of mice induced by SCOP had inflammation, and after intervention with Lactobacillus plantarum LZU-LJY-1Q1, the inflammation was significantly improved.

[0091] 4.6 Enzyme-linked immunosorbent assay

[0092] 4.6.1 Investigation of anti-inflammatory effects

[0093] The blood was taken from the mouse eyeball and allowed to stand for half an hour before centrifugation (4°C, 4000r / min, 10min) to separate the serum, which was rapidly cooled in liquid nitrogen and placed in a -80°C refrigerator for testing. Interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured according to the kit instructions. The results are shown in Figure 7 As shown, compared with group C, IL-1β, TNF-α and IL-6 in group M were increased, while the content of inflammatory factors in group L was significantly reduced compared with group M. The results showed that Lactobacillus plantarum LZU-LJY-1Q1 improved the memory of mice by reducing inflammatory damage induced by SCOP.

[0094] 4.6.2 Investigation of improving oxidative stress

[0095] Serum detection of antioxidant indicators malondialdehyde (MDA) and total superoxide dismutase (SOD). Mice were killed by cervical dislocation and their heads were removed. The whole brain of the mouse was removed on ice as soon as possible, and the hippocampal tissue was quickly transferred to a 2mL centrifuge tube, placed in liquid nitrogen for rapid freezing, and stored in a -80℃ refrigerator. The hippocampal tissue was mixed with physiological saline at a ratio of 1:9 (g:mL), ground, and centrifuged at 4℃ (4000r / min, 10min) to obtain the supernatant, and the reduced glutathione (GSH), oxidized glutathione (GSSH), SOD and MDA were determined according to the instructions of the kit. GSSG is the oxidized form of GSH, and GSSG / GSH can reflect the level of oxidative stress. The results are as follows Figure 8 As shown, compared with mice in group C, the SOD activity and GSH content of mice treated with SCOP were significantly reduced, and the MDA content was increased; pretreatment in group L could reduce the content of GSH / GSSG and MDA. The results showed that pretreatment with Lactobacillus plantarum LZU-LJY-1Q1 could inhibit SCOP-induced oxidative stress in mice and improve memory impairment.

[0096] 4.6.3 Study on improving cholinergic system disorders

[0097] The mice were killed by dislocating the neck and the heads were removed. The whole brain of the mice was removed on ice as soon as possible, the hippocampal tissue was peeled out and quickly transferred to a 2 mL centrifuge tube, placed in liquid nitrogen for rapid freezing, and stored in a -80°C refrigerator. The hippocampal tissue was mixed with physiological saline at a ratio of 1:9 (g:mL), ground and centrifuged at 4°C (4000r / min, 10min) to obtain the supernatant, and acetylcholine (AcH) and acetylcholinesterase (AChE) were determined according to the instructions of the kit. The cholinergic system of the brain plays a central role in learning and memory. AcH is a neurotransmitter of the cholinergic system. In addition, increased AChE activity can also lead to persistent AcH deficiency and cognitive impairment. The results are as follows Fig. 9As shown in the figure, compared with group C, the AChE activity in the hippocampus of mice in group M was increased and the AcH activity was decreased, indicating that the cholinergic nervous system in group M was disordered. However, Lactobacillus plantarum LZU-LJY-1Q1 reduced the AChE activity and increased the AcH level in SCOP-induced mice. The results showed that Lactobacillus plantarum LZU-LJY-1Q1 could prevent the imbalance of the cholinergic system in SCOP-treated mice and improve memory impairment.

[0098] In summary, Lactobacillus plantarum LZU-LJY-1Q1 improves SCOP-induced memory loss by balancing the cholinergic system, reducing neuroinflammation and oxidative stress, and protecting neurons and intestinal morphology.

[0099] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of Lactobacillus plantarum LZU-LJY-1Q1 in the preparation of a medicine for improving impaired memory, characterized in that: The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCCNO:64797.

2. The use according to claim 1, characterized in that: The Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory ability by balancing the cholinergic system.

3. The use according to claim 1, characterized in that: The Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory ability by reducing neuroinflammation.

4. The use according to claim 1, characterized in that: The Lactobacillus plantarum LZU-LJY-1Q1 improves impaired memory ability by reducing oxidative stress.

5. Application of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food for assisting in improving memory, characterized in that: The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO:64797.

6. Use of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food that helps regulate intestinal flora, characterized in that: The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCCNO:64797.

7. Use of Lactobacillus plantarum LZU-LJY-1Q1 in preparing food that helps to resist oxidation, characterized in that: The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO:64797.

8. The use according to any one of claims 5 to 7, characterized in that: The food includes health food.

9. The use according to any one of claims 1 to 8, characterized in that: The method of using the Lactobacillus plantarum LZU-LJY-1Q1 comprises preparing the Lactobacillus plantarum LZU-LJY-1Q1 into a bacterial suspension; The number of viable bacteria in the bacterial suspension is ≥ 1×10 7 CFU / ml.

10. Application of Lactobacillus plantarum LZU-LJY-1Q1 in the preparation of cosmetics, characterized in that: The deposit number of the Lactobacillus plantarum LZU-LJY-1Q1 is GDMCC NO:64797.

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