Antibacterial composition and application thereof

Abstract

The invention provides an antibacterial composition and application thereof, and belongs to the technical field of antibacterial drugs. The antibacterial composition provided by the invention is prepared from a banana peel extract and SU3327. The banana peel extract and the SU3327 are combined for use, so that escherichia coli, staphylococcus aureus and klebsiella pneumoniae can be effectively killed or inhibited, the use amount of the SU3327 in treatment of bacterial infection is greatly reduced, and the application value is extremely high in the process of treating the bacterial infection.

Description

Technical Field

[0001] The present invention relates to the technical field of antibacterial drugs, and in particular to an antibacterial composition and application thereof. Background Art

[0002] Bacteria are one of the main causes of disease in humans and animals. The most effective treatment for bacterial infections is generally the use of antibiotics, but the long-term use of antibiotics has led to an increasingly serious problem of bacterial resistance, and even to the point where no drugs are available. The game between antibiotics and bacteria is a question of whether the "post-antibiotic era" will emerge. The development and spread of bacterial resistance is a race against the development of new antibiotics. The development of new antibacterial drugs has become one of the important ways to curb bacterial resistance in clinical practice. However, the research and development of new antibiotics lags far behind the rapid evolution and spread of antibiotic resistance. Compared with the discovery of new antimicrobial drugs, combined strategies can restore the sensitivity of resistant strains to existing antibiotics, thus bringing hope to clinical treatment.

[0003] SU3327, CAS No. 40045-50-9, molecular formula C5H3N5O2S3, molecular weight 261.3, is a C-JUN N-terminal kinase (JNK) selective inhibitor, also known as halicin or SU3327, is a new potential antibiotic developed by a research team led by James Collins of the Massachusetts Institute of Technology (MIT) using deep learning artificial intelligence (AI). It has a certain inhibitory effect on a variety of bacteria such as Acinetobacter baumannii, Escherichia coli, Mycobacterium tuberculosis, and Clostridium difficile (Stokes JM, Yang K, Swanson K, Jin W, Cubillos-Ruiz A, Donghia NM, MacNair CR, French S, Carfrae LA, Bloom-Ackermann Z, Tran VM, Chiappino-Pepe A, Badran AH, Andrews IW, Chory EJ, Church GM, Brown ED, Jaakkola TS, Barzilay R, Collins JJ.A Deep Learning Approach to Antibiotic Discovery. Cell. 2020, 180(4): 688-702); in the authorized patent CN202110710023.1, the inhibitory effect of SU3327 on Actinobacillus pleuropneumoniae was further reported (patent application number: CN202310092613.1); subsequently, SU3327 was reported on the synergistic effect of colistin against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus pseudintermedius containing colistin resistance gene 1 (MCR1), as well as the synergistic effect of vancomycin against enterococci (patent application number: CN202310568344.1). In the patent CN202410131623.6, it was found that SU3327 combined with the antibacterial drug colistin had a good synergistic effect. In addition, long-term use of SU3327 may produce certain toxic effects.

[0004] Banana belongs to the genus Musa of the family Musaceae. It is the world's second largest fruit after citrus, accounting for about 16% of the world's total fruit production. The world's total production in 2018 was about 116 million tons. my country is rich in banana resources. Banana peel, as a by-product of bananas, accounts for 30% to 40% of the fresh weight of bananas. However, the utilization rate of banana peels is very low. They are usually used as organic fertilizers or even directly discarded as garbage, which not only wastes resources but also pollutes the environment. Previous studies have found that the main components of banana peel methanol and ethanol extracts are similar, including pyrogallic acid, cis-9-hexadecenal, pentadecanoic acid, benzoic acid, etc., which have broad-spectrum inhibitory effects on Gram-positive and Gram-negative bacteria, yeasts, and molds, but the minimum inhibitory concentration (MIC) is relatively high, about 300 mg / mL (Reference: NIAMAH A K. Determination, identification of bioactive compounds extracts from yellow banana peels and used in vitro as antimicrobial [J]. International journal of phytomedicine, 2014, 6 (4): 625-632).

[0005] So far, there is no literature report on banana peel as an antibacterial adjuvant to enhance the synergy of SU3327. The present invention studies the antibacterial activity of the combined application of banana peel and SU3327, which provides an important basis for the combined use of the two for the treatment of clinical bacterial infections, and has important significance. Summary of the invention

[0006] The object of the present invention is to provide an antibacterial composition and application thereof, which significantly improves the antibacterial effect through the synergistic effect of banana peel extract and SU3327 when used in combination.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The invention provides an antibacterial composition, characterized in that it comprises SU3327 and banana peel extract.

[0009] Preferably, the preparation method of the banana peel extract is:

[0010] (1) selecting fresh banana peels, drying them, crushing them, and sieving them to obtain banana peel fine powder;

[0011] (2) mixing the banana peel powder with an ethanol aqueous solution and performing ultrasonic extraction to obtain an extract;

[0012] (3) filtering the extract, concentrating, and freeze-drying to obtain a banana peel extract.

[0013] Preferably, the drying temperature in step 1 is 50-60° C. and the drying time is 30-33 hours; and the mesh size of the sieve is 80-100 meshes.

[0014] Preferably, the volume concentration of the ethanol aqueous solution in step 2 is 60-70%, and the mass volume ratio of the banana peel powder to the ethanol aqueous solution is 1:40-45 g / ml.

[0015] Preferably, the ultrasonic extraction in step 2 is performed 2 to 3 times, the power of each ultrasonic extraction is 400 to 450 W, the ultrasonic time is 35 to 45 min, the temperature during the ultrasonic extraction is 30 to 35° C., and after each ultrasonic extraction, the mixture is allowed to stand for 10 to 15 min to obtain the supernatant, and the supernatants are combined to obtain the extract.

[0016] Preferably, in the step (3), the extract is concentrated to be in the form of an extract, and the temperature of the cold trap for freeze drying is -80°C and the pressure is 60Pa.

[0017] The present invention also provides an application of the antibacterial composition in preparing an antibacterial agent.

[0018] Preferably, the antibacterial agent is used to kill Gram-positive bacteria and Gram-negative bacteria.

[0019] Preferably, the Gram-positive bacteria include Staphylococcus aureus; and the Gram-negative bacteria include Escherichia coli and Klebsiella pneumoniae.

[0020] The present invention also provides an antibacterial agent for use, wherein the antibacterial agent further comprises a pharmaceutically acceptable carrier; the dosage form of the antibacterial agent comprises a premix, tablet, oral solution, capsule, injection, pellet or lyophilized powder.

[0021] The antibacterial composition provided by the present invention can effectively kill or inhibit Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae by using SU3327 and banana peel extract in combination, and greatly reduces the usage of SU3327 in the treatment of bacterial infection, showing extremely strong application value in the process of treating bacterial infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The results of the synergistic antibacterial chessboard method of SU3327 combined with banana peel extract against Escherichia coli 25922 in Example 1;

[0023] Figure 2 The results of the synergistic antibacterial chessboard method of SU3327 combined with banana peel extract against Escherichia coli B2 in Example 1;

[0024] Figure 3The results of the synergistic antibacterial chessboard method of SU3327 combined with banana peel extract against Staphylococcus aureus 33591 in Example 1;

[0025] Figure 4 The results of the synergistic antibacterial chessboard method of SU3327 combined with banana peel extract against Klebsiella pneumoniae 1202 in Example 1;

[0026] Figure 5 The sterilization curve results of SU3327 combined with banana peel extract against Escherichia coli 25922 in Example 3;

[0027] Figure 6 The sterilization curve results of SU3327 combined with banana peel extract against Escherichia coli B2 in Example 3;

[0028] Figure 7 This is the sterilization curve result of SU3327 combined with banana peel extract against Staphylococcus aureus 33591 in Example 3. DETAILED DESCRIPTION

[0029] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0030] Preparation of banana peel extract

[0031] Fresh banana peels were selected, dried in an oven at 60°C for 30 hours, crushed with a pulverizer, passed through an 80-mesh sieve, and the sieved material was taken to obtain banana peel fine powder; banana peel fine powder was mixed with an ethanol aqueous solution with a volume concentration of 66% according to a solid-liquid ratio of 1g:42ml, and ultrasonic assisted extraction was performed at 30°C with a power of 430W, and the ultrasonic time was 40min. The supernatant was taken, precipitated and ultrasonicated twice, and the three supernatants were combined to obtain an extract, and the extract was filtered using a glass funnel and filter paper. When filtering, the circular filter paper was folded into four layers, and the filter paper was stretched into a cone, with one side being a single layer and the other side being three layers, and placed in a funnel. The filtrate was concentrated to an extract using a rotary evaporator, and the banana peel extract was obtained after freeze drying under the conditions of a cold trap temperature of -80°C and a pressure of 60Pa.

[0032] The SU3327 used in this application was purchased from MedChemExpress with a purity of 98.03%. Before use, the concentration of SU3327 needed to be adjusted to 10 mg / mL using dimethyl sulfoxide (DMSO) and placed in a -20°C refrigerator for later use.

[0033] The bacteria used in this application are Staphylococcus aureus 33591, Klebsiella pneumoniae 1202, Escherichia coli 25922, and Escherichia coli B2, all of which are from the National Center for Veterinary Drug Safety (Beijing).

[0034] The Mueller HintonⅡCationAdjusted broth (CAMHB broth) medium used in this application was purchased from BD Biosciences (Shanghai) Co., Ltd. The preparation method is as follows: weigh 22.0 g of this product in 1 L of distilled water, heat and boil until completely dissolved, sterilize at 121°C for 15 min, and set aside;

[0035] The MH agar medium used in this application was purchased from Beijing Luqiao Technology Co., Ltd. The preparation method is as follows: weigh 38.0 g of the product in 1 L of distilled water, heat and boil until completely dissolved, sterilize at 121°C under high pressure for 15 min, cool to 55°C and pour into a plate for later use.

[0036] The brain heart infusion broth medium (BHI broth medium) used in this application was purchased from Beijing Luqiao Technology Co., Ltd. The preparation method is as follows: weigh 38.5 g of this product, dissolve it in 1000 mL of distilled water with heating and stirring, adjust the pH to 7.3, and sterilize it under high pressure at 121°C for 15 min for use.

[0037] Example 1 Effects of SU3327 and banana peel extract alone and in combination against Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae

[0038] 1. Inoculate Staphylococcus aureus 33591, Escherichia coli B2, Klebsiella pneumoniae 1202, and Escherichia coli A25922 (ATCC25922) into BHI broth medium for bacterial enrichment culture at 37°C, 200 rpm, and culture for 6 hours to obtain bacterial liquid;

[0039] 2. The effects of SU3327 and banana peel extract alone and in combination on various bacteria were investigated using the chessboard method. The specific steps were as follows: SU3327 and banana peel extract were taken separately, and dimethyl sulfoxide (DMSO) was used to prepare 40 μg / mL SU3327 and 25 mg / mL banana peel extract solutions, which were then diluted in multiples using CAMHB broth to obtain banana peel extracts with concentrations of 25 mg / mL, 12.5 mg / mL, 6.25 mg / mL, and 3.125 mg / mL, respectively. g / mL, 1.563mg / mL, 0.781mg / mL, 0.391mg / mL, 0.195mg / mL, 0.098mg / mL, 0.04882mg / mL, 0.02441mg / mL, SU3327 concentrations were 40μg / mL, 20μg / mL, 10μg / mL, 5μg / mL, 2.5μg / mL, 1.25μg / mL, 0.625μg / mL; the control group was given an equal volume of DMSO, where the final concentration of DMSO did not exceed 1%. 50μL of CAMHB broth containing different concentrations of SU3327 and banana peel extract were added along the horizontal and vertical axes of the 96-well culture plate, and then 50μL of various pathogenic bacteria were added, so that the final number of bacteria in each well was 2×10 5 CFU, incubate in a 37°C constant temperature incubator for 24 hours, and observe the results; record the minimum inhibitory concentration (MIC) of the two drugs when used alone and in combination, and calculate the FICI value (fractional inhibitory concentration index) according to the following formula:

[0040] FICI of SU3327 and banana peel extract = MIC (SU3327 combined) / MIC (SU3327 alone) + MIC (banana peel extract combined) / MIC (banana peel extract alone); judgment criteria: FICI ≤ 0.5, synergistic effect; 0.5 < FICI ≤ 1, additive effect; 1 < FICI ≤ 2, no effect; FICI > 2, antagonistic effect. The test results are as follows Figures 1 to 4 shown.

[0041] from Figure 1 It can be seen that against Escherichia coli 25922, the MICs of SU3327 and banana peel extract alone were 20 μg / mL and greater than 25 mg / mL, respectively. When the two were used in combination, SU3327 was in the range of 2.5-5 μg / mL, and banana peel extract was in the range of 3.125 mg / mL-12.5 mg / mL, and the two had obvious synergistic effects; when SU3327 was at 5 μg / mL and banana peel extract was at 3.125 mg / mL, the two achieved the best synergistic effect, and the synergistic index FICI was 0.3125.

[0042] from Figure 2It can be seen that for Escherichia coli B2, the MICs of SU3327 and banana peel extract alone were 20 μg / mL and greater than 25 mg / mL, respectively. When the two were used together, SU3327 was at 5 μg / mL and banana peel extract was in the range of 6.25 mg / mL-12.5 mg / mL, and the two had obvious synergistic effects; when SU3327 was at 5 μg / mL and banana peel extract was at 6.25 mg / mL, the two achieved the best synergistic effect, and the synergistic index FICI was 0.375.

[0043] from Figure 3 It can be seen that for Staphylococcus aureus 33591, the MICs of SU3327 and banana peel extract alone were 20 μg / mL and 25 mg / mL, respectively. When the two were used in combination, SU3327 was in the range of 2.5-5 μg / mL and banana peel extract was in the range of 1.5625 mg / mL-6.25 mg / mL, and the two had obvious synergistic effects; when SU3327 was at 2.5 μg / mL and banana peel extract was at 3.125 mg / mL, the two achieved the best synergistic effect, and the synergistic index FICI was 0.25.

[0044] from Figure 4 It can be seen that for Klebsiella pneumoniae 1202, the MICs of SU3327 and banana peel extract alone were 40 μg / mL and greater than 25 mg / mL, respectively. When the two were used in combination, SU3327 was at 20 μg / mL and banana peel extract was in the range of 12.5 mg / mL-25 mg / mL, and the two had an obvious additive effect; when SU3327 was at 20 μg / mL and banana peel extract was at 12.5 mg / mL, the two achieved the best additive effect, and the synergistic index FICI was 0.75.

[0045] In conclusion, SU3327 combined with banana peel extract had obvious synergistic and additive antibacterial effects against Escherichia coli A25922 (ATCC25922), Escherichia coli B2, Staphylococcus aureus 33591, and Klebsiella pneumoniae 1202, with FICI ranging from 0.25 to 0.75.

[0046] Example 2 Determination of the minimum bactericidal combination concentration (MBC) of SU3327 and banana peel extract used alone and in combination

[0047] 10 μL of the solution from each well of the 96-well culture plate in Example 1 where no bacteria grew was spotted on MH agar medium and cultured in a 37°C constant temperature incubator for 18 h. The lowest concentration in the well where no bacteria grew was the MBC. The measurement results are shown in Table 1.

[0048] Table 1 MBC values ​​of SU3327 and banana peel extract alone or in combination against various bacteria

[0049]

[0050] As can be seen from Table 1, against Escherichia coli A25922 (ATCC25922), the MBCs of SU3327 and banana peel extract alone were 40 μg / mL and greater than 25 mg / mL, respectively, and the minimum bactericidal combination concentrations of the two were 5 μg / mL and 3.125 mg / mL, respectively;

[0051] As can be seen from Table 1, for E. coli B2, the MBCs of SU3327 and banana peel extract alone were 40 μg / mL and greater than 25 mg / mL, respectively, and the minimum bactericidal combination concentrations of the two were 5 μg / mL and 6.25 mg / mL, respectively;

[0052] As can be seen from Table 1, against S. aureus 33591, the MBCs of SU3327 and banana peel extract alone were 40 μg / mL and 25 mg / mL, respectively, and the minimum bactericidal combination concentrations of the two combined were 2.5 μg / mL and 3.125 mg / mL, respectively;

[0053] As can be seen from Table 1, for Klebsiella pneumoniae 1202, the MBCs of SU3327 and banana peel extract alone were greater than 40 μg / mL and greater than 25 mg / mL, respectively, and the minimum bactericidal combination concentrations of the two combined were 20 μg / mL and 25 mg / mL and 40 μg / mL and 6.25 mg / mL, respectively;

[0054] In summary, the combined treatment of SU3327 and banana peel extract had a significant bactericidal effect on Escherichia coli A25922 (ATCC25922), Escherichia coli B2, Staphylococcus aureus 33591, and Klebsiella pneumoniae 1202, and significantly reduced the dosage of SU3327 and banana peel extract.

[0055] Example 3 Evaluation of the bactericidal effect of SU3327 and banana peel extract alone and in combination against Escherichia coli B2, Escherichia coli 25922 and Staphylococcus aureus 33591

[0056] Escherichia coli A25922 (ATCC25922) was inoculated into BHI broth medium for enrichment culture at 37°C, 200 rpm, and cultured for 6 hours to obtain bacterial liquid; the bacterial liquid was divided into 4 groups: 20 μg / mL SU3327 group, 12.5 mg / mL banana peel extract group, 12.5 mg / mL banana peel extract + 20 μg / mL SU3327 group and control group;

[0057] SU3327 was added to the bacterial solution of the 20 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 20 μg / mL; banana peel extract was added to the bacterial solution of the 12.5 mg / mL banana peel extract group to prepare a mixed solution, wherein the final concentration of banana peel extract was 12.5 mg / mL; SU3327 and banana peel extract were added to the bacterial solution of the 12.5 mg / mL banana peel extract + 20 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 20 μg / mL, and the concentration of banana peel extract was 12.5 mg / mL; the control group added 1% DMSO as a solvent control.

[0058] The mixed solution was placed in a 37°C constant temperature incubator for culture. After 1 h, 3 h, 6 h, 12 h, and 24 h of culture, 100 μL of each mixed solution was spread on a 10 cm diameter MH agar medium. After overnight culture, the colonies were counted. The statistical results are shown in the table below. Figure 5 shown.

[0059] Depend on Figure 5 It can be seen that for Escherichia coli 25922 strain, at the 24th hour, compared with the control group, the treatment with SU3327 and banana peel extract alone had no significant effect on the bacterial colony count; when SU3327 and banana peel extract were treated together, at the 3rd hour of treatment, the colony count was significantly lower than that of the control group and the group treated with banana peel extract alone, until 12 hours, there was no bacterial growth and the colony count was 0; therefore, the combined treatment of SU3327 and banana peel extract had an obvious synergistic bactericidal effect on Escherichia coli 25922.

[0060] Escherichia coli B2 was inoculated into BHI broth medium for enrichment culture at 37°C, 200 rpm, and cultured for 6 hours to obtain bacterial liquid; the bacterial liquid was divided into 4 groups: 20 μg / mL SU3327 group, 25 mg / mL banana peel extract group, 25 mg / mL banana peel extract + 20 μg / mL SU3327 group and control group;

[0061] SU3327 was added to the bacterial solution of the 20 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 20 μg / mL; banana peel extract was added to the bacterial solution of the 25 mg / mL banana peel extract group to prepare a mixed solution, wherein the final concentration of banana peel extract was 25 mg / mL; SU3327 and banana peel extract were added to the bacterial solution of the 25 mg / mL banana peel extract + 20 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 20 μg / mL, and the concentration of banana peel extract was 25 mg / mL;

[0062] The mixed solution was placed in a 37°C constant temperature incubator for culture. After 1 h, 3 h, 6 h, 12 h, and 24 h of culture, 100 μL of each mixed solution was spread on a 10 cm diameter MH agar medium. After overnight culture, the colonies were counted. The statistical results are shown in the table below. Figure 6 shown.

[0063] Depend on Figure 6 It can be seen that for Escherichia coli B2 strain, at the 24th hour, compared with the control group, the treatment with SU3327 and banana peel extract alone had no significant effect on the bacterial colony count; when SU3327 and banana peel extract were treated together, at the 3rd hour of treatment, the colony count was significantly lower than that of the control group and the group treated with banana peel extract alone, until 12 hours, there was no bacterial growth and the colony count was 0; therefore, the combined treatment of SU3327 and banana peel extract had an obvious synergistic bactericidal effect on Escherichia coli B2.

[0064] Staphylococcus aureus 33591 was inoculated into BHI broth medium for enrichment culture at 37°C, 200 rpm, and cultured for 6 hours to obtain bacterial liquid; the bacterial liquid was divided into 4 groups: 10 μg / mL SU3327 group, 12.5 mg / mL banana peel extract group, 12.5 mg / mL banana peel extract + 10 μg / mL SU3327 group and control group;

[0065] SU3327 was added to the bacterial solution of the 20 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 10 μg / mL; banana peel extract was added to the bacterial solution of the 12.5 mg / mL banana peel extract group to prepare a mixed solution, wherein the final concentration of the banana peel extract was 12.5 mg / mL; SU3327 and banana peel extract were added to the bacterial solution of the 12.5 mg / mL banana peel extract + 10 μg / mL SU3327 group to prepare a mixed solution, wherein the final concentration of SU3327 was 10 μg / mL, and the concentration of banana peel extract was 12.5 mg / mL;

[0066] The mixed solution was placed in a 37°C constant temperature incubator for culture. After 1 h, 3 h, 6 h, 12 h, and 24 h of culture, 100 μL of each mixed solution was spread on a 10 cm diameter MH agar medium. After overnight culture, the colonies were counted. The statistical results are shown in the table below. Figure 7 shown.

[0067] Depend on Figure 7It can be seen that for Staphylococcus aureus 33591 strain, at the 24th hour, compared with the control group, the treatment with SU3327 and banana peel extract alone had no significant effect on the bacterial colony count; when SU3327 and banana peel extract were treated together, at the 3rd hour of treatment, the colony count was significantly lower than that of the control group and the group treated with banana peel extract alone, until 12 hours, there was no bacterial growth and the colony count was 0; therefore, the combined treatment of SU3327 and banana peel extract had an obvious synergistic bactericidal effect on Escherichia coli 25922.

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An antibacterial composition, characterized in that Includes SU3327 and banana peel extract.

2. The antibacterial composition according to claim 1, characterized in that The preparation method of the banana peel extract is: (1) selecting fresh banana peels, drying them, crushing them, and sieving them to obtain banana peel fine powder; (2) mixing the banana peel powder with an ethanol aqueous solution and performing ultrasonic extraction to obtain an extract; (3) filtering the extract, concentrating, and freeze-drying to obtain a banana peel extract.

3. The antibacterial composition according to claim 2, characterized in that The drying temperature in step 1 is 50-60° C. and the drying time is 30-33 hours; the mesh number of the sieving sieve is 80-100 meshes.

4. The antibacterial composition according to claim 2, characterized in that The volume concentration of the ethanol aqueous solution in step 2 is 60-70%, and the mass volume ratio of the banana peel powder to the ethanol aqueous solution is 1:40-45 g / ml.

5. The antibacterial composition according to claim 2, characterized in that The ultrasonic extraction in step 2 is performed 2 to 3 times, the power of each ultrasonic extraction is 400 to 450 W, the ultrasonic time is 35 to 45 minutes, the temperature during the ultrasonic extraction is 30 to 35° C. After each ultrasonic extraction, the supernatant is taken after standing for 10 to 15 minutes, and the supernatants are combined to obtain the extract.

6. The antibacterial composition according to claim 2, characterized in that In the step (3), the extract is concentrated to a paste-like state, and the temperature of the freeze-drying cold trap is -80°C and the pressure is 60Pa.

7. Use of the antibacterial composition according to any one of claims 1 to 6 in the preparation of an antibacterial agent.

8. The use according to claim 7, characterized in that The antimicrobial agent is used to kill Gram-positive bacteria and Gram-negative bacteria.

9. The use according to claim 8, characterized in that The Gram-positive bacteria include Staphylococcus aureus; the Gram-negative bacteria include Escherichia coli and Klebsiella pneumoniae.

10. An antibacterial agent for use according to claim 9, characterized in that: The antibacterial agent also includes a pharmaceutically acceptable carrier; the dosage form of the antibacterial agent includes a premix, cream, powder, tablet, oral solution, capsule, injection, pill or freeze-dried powder.

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