Traditional Chinese medicine composition for liver conditioning

Abstract

The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine components in parts by weight: 35 to 38 parts of fingered citron, 30 to 32 parts of fructus gardeniae, 25 to 28 parts of folium mori, 22 to 25 parts of flos chrysanthemi, 18 to 20 parts of lotus leaves, 14 to 16 parts of mulberries, 10 to 12 parts of radix polygonati officinalis, 8 to 10 parts of fructus lycii, 5 to 6 parts of fructus crataegi, 3 to 5 parts of pericarpium citri reticulatae, 3 to 4 parts of semen ziziphi spinosae and 2 to 4 parts of fructus momordicae. The preparation method comprises the following steps: drying and crushing traditional Chinese medicines, adding water, polyglycerol fatty acid ester and cellulase, treating in ultrasonic equipment, decocting, filtering, adding erythritol, nisin and concentrated lemon juice, and decocting to obtain the traditional Chinese medicine composition. The prepared traditional Chinese medicine composition is high in effective component content, strong in medicine effect and good in taste, the liver conditioning problems such as liver protection and liver protection can be solved, and the adopted traditional Chinese medicines are all medicinal and edible substances, have no side effects on human bodies and can be taken for a long time.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine preparations, and in particular to a Chinese medicine composition for liver conditioning. Background Art

[0002] With the development of society and the changes in modern lifestyles, such as unreasonable eating habits, lack of exercise, frequent staying up late, excessive drinking and smoking, overwork and other factors, the incidence of liver disease continues to rise and is getting younger and younger. Symptoms such as loss of appetite, increased fatigue, yellowing of the skin, and abdominal distension are often warning signs of liver disease. Therefore, there is a huge market demand for the development of products with liver conditioning functions such as liver protection and liver protection.

[0003] Traditional Chinese medicine has a long history and rich clinical experience. Many Chinese medicines have been proven to have liver-protecting effects such as antioxidant, anti-inflammatory, and promoting liver cell repair. However, the Chinese medicines currently on the market have unstable efficacy, low content of effective ingredients, and relatively poor taste.

[0004] Therefore, there is an urgent need to provide a Chinese medicine composition for liver conditioning to solve liver conditioning problems such as liver protection and liver care. Summary of the invention

[0005] The object of the present invention is to provide a Chinese medicine composition for liver conditioning to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a Chinese medicine composition for liver conditioning, which is prepared by boiling and concentrating the following components in parts by weight: 68-75 parts of Chinese medicine decoction, 6-8 parts of erythritol, 4-5 parts of nisin, and 3-4 parts of concentrated lemon juice.

[0007] The Chinese medicine decoction is prepared by adding water to the following components in parts by weight, and then ultrasonically treating and decocting: 35-38 parts of bergamot, 30-32 parts of gardenia, 25-28 parts of mulberry leaves, 22-25 parts of chrysanthemum, 18-20 parts of lotus leaves, 14-16 parts of mulberry, 10-12 parts of polygonatum, 8-10 parts of wolfberry, 5-6 parts of hawthorn, 3-5 parts of tangerine peel, 3-4 parts of spiny jujube seeds, 2-4 parts of momordica, 1.5-3 parts of polyglycerol fatty acid ester, and 1-2 parts of cellulase. The amount of water added is 4-5 times the total weight of the bergamot, gardenia, mulberry leaves, chrysanthemum, lotus leaves, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seeds, and momordica.

[0008] A Chinese medicine composition for liver conditioning is prepared by the following preparation method:

[0009] S1. Grinding of Chinese medicine: Grind bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed and monk fruit in a grinder respectively, so that the particle size of the Chinese medicine is less than or equal to 1.2mm.

[0010] S2. Ultrasonic treatment of traditional Chinese medicine: Add water, polyglycerol fatty acid ester and cellulase into ultrasonic equipment in sequence and mix evenly, add acid-base regulator to adjust the pH value to 6.0-6.5, and then add crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube kernel and monk fruit in proportion, and treat with ultrasound for 30-35 minutes to obtain a solid-liquid mixture of traditional Chinese medicine.

[0011] S3. Decoction of Chinese medicine: Place the solid-liquid mixture of Chinese medicine in an electric heating double-layer pot, bring to a boil over high heat, then simmer over low heat for 50-60 minutes, filter to obtain a primary decoction, add water to the Chinese medicine residue after the primary decoction, and decoct in an amount of 3-4 times the total weight of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube seed, and momordica grosvenori, bring to a boil over high heat, then simmer over low heat for 50-60 minutes, filter to obtain a secondary decoction, and mix the two decoctions to obtain a Chinese medicine decoction.

[0012] S4. Preparation of Chinese medicine: Place the Chinese medicine decoction into an electric heating double-layer pot, add erythritol, nisin, and concentrated lemon juice, and simmer over low heat until concentrated by 2 to 2.5 times to obtain a Chinese medicine composition.

[0013] As a preferred technical solution of the present invention, before pulverizing bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and momordica grosvenori, the method further comprises: drying bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and momordica grosvenori at 50-55° C. to make the moisture content of the Chinese medicine reach 7-9%.

[0014] As a preferred technical solution of the present invention, the temperature of the ultrasonic equipment is set to 37-42° C. and the frequency is set to 110-115 Hz.

[0015] As a preferred technical solution of the present invention, the acid-base regulator is at least one of citric acid and tartaric acid.

[0016] As a preferred technical solution of the present invention, the cellulase activity is 30-35 U / mg.

[0017] As a preferred technical solution of the present invention, the concentration of concentrated lemon juice is 5 to 6 times.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. When the Chinese medicines such as bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube seed and monk fruit are treated with ultrasonic equipment, cellulase is added to degrade the cellulose components in the cell wall of Chinese medicine, making the cell wall structure loose, thereby reducing the mass transfer resistance of the effective ingredients from the cell to the extraction medium, and promoting the dissolution of the effective ingredients of Chinese medicine. Polyglycerol fatty acid esters can promote the rupture of plant cell walls and the release of effective ingredients, thereby improving the solubility and extraction efficiency of effective ingredients in Chinese medicine. At the same time, polyglycerol fatty acid esters can prevent the Chinese medicine extract from stratification and precipitation during the boiling and storage process, prevent the bottom of the sandwich pot from sticking to the pot, burning and other problems during the boiling process, and maintain the uniformity and stability of the Chinese medicine extract. The cavitation effect generated by ultrasound can destroy the cell structure of Chinese medicine, making the effective ingredients of Chinese medicine easier to dissolve. Adding cellulase and polyglycerol fatty acid esters to traditional Chinese medicine during the ultrasonic treatment process can more effectively promote the dissolution of traditional Chinese medicine ingredients, reduce the subsequent decoction time of traditional Chinese medicine, increase the active ingredients in the traditional Chinese medicine composition, and thus enhance the efficacy.

[0020] 2. The present invention is to scientifically match bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed, and momordica grosvenori, and make the effective active ingredients of traditional Chinese medicine, such as flavonoids, polysaccharides, gardenia glycosides, alkaloids, etc., into palatable traditional Chinese medicine paste through ultrasonic treatment, boiling, boiling and other processes, improve fatty liver, hyperlipidemia, irritability, long-term staying up late and other problems, play the role of nourishing the liver, protecting the liver, and improving immunity. At the same time, the traditional Chinese medicines used in the present invention are all medicine and food homologous substances, have no side effects on the human body, and can be taken for a long time.

[0021] 3. Add erythritol during the Chinese medicine boiling process. Erythritol is a natural sugar alcohol that can adjust the taste of Chinese medicine and improve its palatability. Erythritol is not easily oxidized and decomposed in the human body. For people with high blood sugar, it can solve the problem of the taste of Chinese medicine while reducing the impact on their own blood sugar. Nisin is a natural preservative that reduces the odor and deterioration of Chinese medicine during the storage process, thereby indirectly improving the taste of Chinese medicine. During the Chinese medicine boiling process, add an appropriate amount of lemon juice. The acidic environment of lemon juice may promote the occurrence of certain chemical reactions in Chinese medicine, help the polymerization and cross-linking of Chinese medicine ingredients, improve the coagulation of pectin, enhance the coagulation of Chinese medicine ointment, and reduce the boiling time. At the same time, the addition of lemon juice can also improve the taste and flavor of Chinese medicine ointment. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The present invention is a flow chart for preparing the Chinese medicine composition. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Buddha's hand mainly contains volatile oils, flavonoids, polysaccharides, minerals and vitamins. Flavonoids are important active ingredients in Buddha's hand and have multiple biological activities, such as antioxidant, anti-inflammatory, soothing the liver and regulating qi, and stomach pain relief. The polysaccharide components in Buddha's hand have pharmacological effects such as enhancing immunity and anti-tumor.

[0025] Gardenia glycoside is the main active ingredient in Gardenia, which has multiple pharmacological effects. Gardenia also contains nutrients such as gardeniain, crocin, crocetin, gardenia formic acid, gardenia acetic acid, chlorogenic acid, volatile oil, polysaccharide, choline and various trace elements. Gardenia can be used to treat symptoms such as jaundice caused by damp-heat in the liver and gallbladder. It has the effects of protecting the liver and promoting bile secretion, promoting bile secretion and bilirubin excretion, reducing blood bilirubin, and protecting the gastric mucosa.

[0026] The crude protein content in mulberry leaves is more than 20%, and soluble sugars account for 18% to 22% of the dry mulberry leaves. Mulberry leaves also contain flavonoids, polyphenols, polysaccharides, vitamins, amino acids, minerals, etc., which have the effects of anti-oxidation, anti-inflammatory, promoting liver detoxification, protecting the liver, and clearing the liver and improving eyesight. The active ingredients such as flavonoids in mulberry leaves can reduce serum cholesterol and triglyceride levels and play a role in lowering blood lipids. The polysaccharides in mulberry leaves can regulate immune function and enhance human immunity.

[0027] Chrysanthemum contains flavonoids, vitamins, amino acids, etc., which can calm liver yang, clear liver heat, promote liver detoxification, protect liver health, and have antibacterial effects.

[0028] Lotus leaf mainly contains flavonoids, alkaloids, volatile essential oils, nuciferine, citric acid, malic acid, gluconic acid, oxalic acid, succinic acid, etc. It has the effects of clearing away heat and relieving summer heat, protecting the liver, losing weight, lowering blood lipids, calming the mind, moisturizing the intestines and relieving constipation, removing heat, and beautifying the skin.

[0029] Mulberries contain protein, fat, cellulose, carbohydrates, and a variety of vitamins and minerals. The fat in mulberries is mainly linoleic acid, oleic acid, palmitic acid, stearic acid, and a small amount of caprylic acid, nonanoic acid, capric acid, myristic acid, linolenic acid, etc. Mulberries are sweet and cold in nature. They enter the heart, liver, and kidney meridians, and have the effect of nourishing the liver and kidneys, nourishing yin and blood, promoting body fluid and moistening dryness, and moistening the intestines and relieving constipation.

[0030] Polygonatum odoratum mainly contains protein, crude fiber, niacin, flavonoids, polysaccharides, saponins, lily of the valley glycosides, lily of the valley bitter glycosides, camellia phenol, quercetin, mucus, and rich carbohydrates, cellulose, etc. It has the functions of nourishing yin and moistening dryness, nourishing the stomach and promoting fluid, moistening the lungs and relieving cough, anti-oxidation, lowering blood sugar, clearing away heat and resolving phlegm, nourishing yin and qi, improving cardiovascular system, and beautifying the skin.

[0031] Lycium barbarum contains rich vitamins, minerals, amino acids, polysaccharides, flavonoids, carotenoids, total acid, linoleic acid, glycosides, amines, germanium and other active ingredients. It has the functions of nourishing liver and kidney, improving eyesight, enhancing immunity, anti-aging, regulating blood sugar and blood lipids, and anti-tumor.

[0032] Hawthorn contains vitamins, minerals, dietary fiber, organic acids, flavonoids, and dietary fiber. It has the effects of lowering cholesterol, anti-oxidation, promoting blood circulation and removing blood stasis, lowering blood lipids, promoting intestinal peristalsis, and losing weight.

[0033] Tangerine peel mainly contains volatile oil, alkaloids, polysaccharides, pectin, trace elements, alkaloids, and various flavonoid compounds such as nobiletin and nobiletin. It has the effects of anti-oxidation, anti-tumor, lowering cholesterol, antibacterial, expectorant, antiasthmatic, regulating qi and strengthening the spleen, promoting the secretion of digestive juice, eliminating intestinal gas, preventing cardiovascular diseases, dilating coronary arteries and promoting bile secretion.

[0034] The Chinese jujube seeds mainly contain saponins and flavonoids, which have the effects of antioxidant, regulating liver qi, regulating cardiovascular, improving sleep, protecting cardiovascular, improving blood circulation, regulating blood pressure and blood lipids, and enhancing immunity.

[0035] Monk fruit contains mogroside, protein, dietary fiber, vitamins, amino acids, polysaccharides, triterpenes, flavonoids, etc. It has the effects of moistening the lungs and relieving cough, protecting the liver, beautifying the skin, and losing weight.

[0036] Currently, the traditional Chinese medicine products for regulating the liver on the market are mainly prepared by mixing and boiling various traditional Chinese medicine components and then drinking directly. Most of the traditional Chinese medicine components used are pure traditional Chinese medicine ingredients. Long-term use will produce other toxic side effects and the taste is relatively poor.

[0037] The invention scientifically combines bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed and momordica grosvenori, and prepares effective active ingredients of traditional Chinese medicine, such as flavonoid compounds, polysaccharides, gardenia glycosides and alkaloids, into a palatable traditional Chinese medicine paste through ultrasonic treatment, boiling, decocting and other processes, so as to improve the problems of fatty liver, hyperlipidemia and long-term staying up late, play the role of nourishing and protecting the liver and improving immunity, and at the same time, the traditional Chinese medicines used are all medicine and food homologous substances, have no side effects on human body, and can be taken for a long time.

[0038] The present invention adds erythritol in the process of boiling Chinese medicine. Erythritol is a natural sugar alcohol with a certain sweetness, which can adjust the taste of Chinese medicine and improve the palatability of Chinese medicine. Erythritol is not easily oxidized and decomposed in the human body, and is mainly converted into glucose through metabolism, but this conversion process is relatively slow, and the influence on blood sugar is small. For people with high blood sugar, the influence on their own blood sugar is reduced while solving the problem of the taste of Chinese medicine. Nisin is a natural preservative, which reduces the peculiar smell and deterioration phenomenon that may be produced in the storage process of Chinese medicine, thereby indirectly improving the taste of Chinese medicine. In the process of boiling Chinese medicine, an appropriate amount of lemon juice is added, and the acidic environment of lemon juice may promote the occurrence of certain chemical reactions in Chinese medicine, help the polymerization and cross-linking of Chinese medicine components, improve the coagulation force of pectin, enhance the coagulation of Chinese medicine paste, reduce the boiling time, and the addition of lemon juice can also improve the taste and flavor of Chinese medicine paste.

[0039] During the ultrasonic treatment of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube seed, and monk fruit, cellulase is added, and the pH value is adjusted to 6.0-6.5, and the temperature is set to 37-42°C. Under the appropriate pH and temperature conditions, cellulase can degrade the cellulose components in the cell wall of Chinese medicine, making the cell wall structure loose, thereby reducing the mass transfer resistance of the effective ingredients from the cell to the extraction medium, and promoting the dissolution of the effective ingredients of Chinese medicine. Polyglycerol fatty acid esters have excellent wettability, emulsification and dispersibility, which can effectively promote the rupture of plant cell walls and the release of effective ingredients, thereby improving the solubility and extraction efficiency of effective ingredients in Chinese medicine. At the same time, polyglycerol fatty acid esters can prevent the occurrence of adverse phenomena such as stratification and precipitation in the process of boiling and storage of Chinese medicine extracts, and prevent the occurrence of problems such as sticking and burning at the bottom of the sandwich pot during boiling, and maintain the uniformity and stability of the Chinese medicine extract. The cavitation effect generated by ultrasound can destroy the cell structure of Chinese medicine, making the effective ingredients of Chinese medicine easier to dissolve. Adding cellulase and polyglycerol fatty acid esters to traditional Chinese medicine during the ultrasonic treatment process can more effectively promote the dissolution of traditional Chinese medicine ingredients, reduce the subsequent decoction time of traditional Chinese medicine, increase the active ingredients in the traditional Chinese medicine composition, and thus enhance the efficacy.

[0040] like Figure 1, a Chinese medicine composition for liver conditioning is prepared by the following preparation method: S1. Drying and crushing Chinese medicine: drying and crushing the Chinese medicine of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed, and monk fruit, so that the moisture content of the Chinese medicine reaches 9%; crushing the dried Chinese medicine in a grinder respectively, so that the particle size of the Chinese medicine is less than or equal to 1.2mm. S2. Ultrasonic treatment of Chinese medicine: adding water, polyglycerol fatty acid ester, and cellulase in an ultrasonic device in sequence and mixing them evenly, adding acid-base regulator citric acid to adjust the pH value to 6.0, and then adding the crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed, and monk fruit in proportion, and obtaining a solid-liquid mixture of Chinese medicine after ultrasonic treatment. S3. Decoction of Chinese medicine: Place the solid-liquid mixture of Chinese medicine in an electric heating double-layer pot, boil it over high heat, then turn to slow heat for decoction, filter to obtain a primary decoction liquid, add water to the Chinese medicine residue after the primary decoction, decoct it, filter to obtain a secondary decoction liquid, and mix the two decoction liquids to obtain a Chinese medicine decoction liquid. S4. Boil Chinese medicine: Place the Chinese medicine decoction liquid in an electric heating double-layer pot, add erythritol, nisin, and concentrated lemon juice, and boil it over low heat to obtain a Chinese medicine composition.

[0041] The raw materials used in the present invention are all commercially available raw materials.

[0042] Embodiment 1:

[0043] The Chinese medicine composition for liver conditioning is prepared by boiling and concentrating the following components in parts by weight: 68 parts of Chinese medicine decoction, 6 parts of erythritol, 4 parts of nisin, and 3 parts of concentrated lemon juice.

[0044] The Chinese medicine decoction is prepared by adding water to the following components in parts by weight, then ultrasonically treating and decocting the components: 35 parts of bergamot, 30 parts of gardenia, 25 parts of mulberry leaf, 22 parts of chrysanthemum, 18 parts of lotus leaf, 14 parts of mulberry, 10 parts of polygonatum, 8 parts of wolfberry, 5 parts of hawthorn, 3 parts of tangerine peel, 3 parts of spiny jujube kernel, 2 parts of momordica, 1.5 parts of polyglycerol fatty acid ester and 1 part of cellulase, and the amount of water added is 4 times the total weight of the Chinese medicines of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and momordica.

[0045] A Chinese medicine composition for liver conditioning is prepared by the following preparation method:

[0046] S1. Drying and crushing Chinese medicine: Dry the Chinese medicines such as bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and monk fruit at 50°C to make the moisture content of the Chinese medicine reach 9%; crush the Chinese medicines such as bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and monk fruit in a grinder respectively to make the particle size of the Chinese medicine less than or equal to 1.2mm.

[0047] S2. Ultrasonic treatment of traditional Chinese medicine: add water, polyglycerol fatty acid ester and cellulase into ultrasonic equipment in sequence and mix evenly, add acid-base regulator citric acid to adjust the pH value to 6.0, and then add crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube kernel and monk fruit in proportion, set the temperature of ultrasonic equipment to 37°C and the frequency to 110 Hz, and perform ultrasonic treatment for 35 minutes to obtain a solid-liquid mixture of traditional Chinese medicine.

[0048] S3. Decoction of Chinese medicine: Place the solid-liquid mixture of Chinese medicine in an electric heating double-layer pot, bring to a boil over high heat, then simmer over low heat for 50-60 minutes, filter to obtain a primary decoction, add water to the Chinese medicine residue after the primary decoction, and decoct in an amount of 3 times the total weight of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube seed, and momordica grosvenori, bring to a boil over high heat, then simmer over low heat for 50 minutes, filter to obtain a secondary decoction, and mix the two decoctions to obtain a Chinese medicine decoction.

[0049] S4. Preparation of Chinese medicine: Place the Chinese medicine decoction into an electric heating double-layer pot, add erythritol, nisin, and 5-fold concentrated lemon juice, simmer over low heat until concentrated by 2 times, and obtain a Chinese medicine composition.

[0050] Embodiment 2:

[0051] The Chinese medicine composition for liver conditioning is prepared by boiling and concentrating the following components in parts by weight: 75 parts of Chinese medicine decoction, 8 parts of erythritol, 5 parts of nisin, and 4 parts of concentrated lemon juice.

[0052] The Chinese medicine decoction is prepared by adding water to the following components in parts by weight, then ultrasonically treating and decocting: 38 parts of bergamot, 32 parts of gardenia, 28 parts of mulberry leaf, 25 parts of chrysanthemum, 20 parts of lotus leaf, 16 parts of mulberry, 12 parts of polygonatum, 10 parts of wolfberry, 6 parts of hawthorn, 5 parts of tangerine peel, 4 parts of spiny jujube kernel, 4 parts of momordica, 3 parts of polyglycerol fatty acid ester and 2 parts of cellulase, and the amount of water added is 5 times the total weight of the Chinese medicines of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and momordica.

[0053] A Chinese medicine composition for liver conditioning is prepared by the following preparation method:

[0054] S1. Drying and crushing Chinese medicine: Dry the Chinese medicines such as bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel, and momordica grosvenori at 55°C to make the moisture content of the Chinese medicine reach 7%; crush the Chinese medicines such as bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel, and momordica grosvenori in a grinder respectively to make the particle size of the Chinese medicine less than or equal to 1.2mm.

[0055] S2. Ultrasonic treatment of traditional Chinese medicine: add water, polyglycerol fatty acid ester and cellulase into ultrasonic equipment in sequence and mix evenly, add acid-base regulator tartaric acid to adjust the pH value to 6.5, and then add crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube kernel and monk fruit in proportion, set the temperature of ultrasonic equipment to 42°C and the frequency to 115Hz, and perform ultrasonic treatment for 30 minutes to obtain a solid-liquid mixture of traditional Chinese medicine.

[0056] S3. Decoction of Chinese medicine: Place the solid-liquid mixture of Chinese medicine in an electric heating double-layer pot, bring to a boil over high heat, then simmer for 60 minutes over low heat, filter to obtain a primary decoction, add water to the Chinese medicine residue after the primary decoction, and decoct in an amount of 4 times the total weight of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube seed, and momordica grosvenori, bring to a boil over high heat, then simmer for 60 minutes over low heat, filter to obtain a secondary decoction, and mix the two decoctions to obtain a Chinese medicine decoction.

[0057] S4. Preparation of Chinese medicine: Place the Chinese medicine decoction into an electric heating double-layer pot, add erythritol, nisin, and lemon juice with a concentration of 6 times, and simmer over low heat until it is concentrated by 2.5 times to obtain a Chinese medicine composition.

[0058] Comparative Example 1:

[0059] The difference from Example 1 is that no cellulase is added.

[0060] Comparative Example 2:

[0061] The difference from Example 1 is that no polyglycerol fatty acid ester is added.

[0062] Comparative Example 3:

[0063] The difference from Example 1 is that in step S2, water, polyglycerol fatty acid ester and cellulase are evenly mixed, acid-base regulator citric acid is added to adjust the pH value to 6.0, and then crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube kernel and momordica grosvenori are added in proportion, the temperature is set to 37° C., and the soaking treatment is carried out for 35 minutes to obtain a solid-liquid mixture of traditional Chinese medicine.

[0064] The total flavonoids in the extract of the Chinese medicine compositions prepared in Examples 1 and 2 and Comparative Examples 1, 2 and 3 were determined according to SN / T4592-2016 "Determination of total flavonoids in exported food", the polysaccharide content of the Chinese medicine compositions was determined by spectrophotometry, and the gardenoside and alkaloid contents of the Chinese medicine compositions were determined by high performance liquid chromatography. The results are shown in Table 1.

[0065] Table 1: Table of active ingredient contents in Chinese medicine compositions

[0066] Total flavonoids (%) Polysaccharide (g / 100g) Gardenia jasminoides (mg / kg) Alkaloids (mg / kg) Embodiment 1 3.56 2.56 0.66 0.19 Embodiment 2 3.84 2.97 0.72 0.2 Comparative Example 1 2.27 2.13 0.46 0.15 Comparative Example 2 2.74 2.35 0.57 0.16 Comparative Example 3 2.91 2.43 0.52 0.17

[0067] As shown in Table 1, the Chinese medicine compositions prepared in Example 1 and Example 2 have high total flavonoids, polysaccharides, gardenia glycosides and alkaloids. The total flavonoids content of the Chinese medicine composition prepared in Example 1 is 36.2%, 23.0% and 18.3% higher than that of the comparative examples 1, 2 and 3, respectively; the polysaccharide content of the Chinese medicine composition prepared in Example 1 is 16.8%, 8.2% and 5.1% higher than that of the comparative examples 1, 2 and 3, respectively; the gardenia glycoside content of the Chinese medicine composition prepared in Example 1 is 30.3%, 13.6% and 21.2% higher than that of the comparative examples 1, 2 and 3, respectively; and the alkaloid content of the Chinese medicine composition prepared in Example 1 is 21.1%, 15.8% and 10.5% higher than that of the comparative examples 1, 2 and 3, respectively.

[0068] It can be seen that when using ultrasonic equipment to treat traditional Chinese medicine, adding cellulase can degrade the cellulose components in the cell wall of traditional Chinese medicine, make the cell wall structure loose, reduce the mass transfer resistance of the effective ingredients from the cell to the extraction medium, and promote the dissolution of the effective ingredients of traditional Chinese medicine. Polyglycerol fatty acid esters can promote the rupture of plant cell walls and the release of effective ingredients, thereby improving the solubility and extraction efficiency of effective ingredients in traditional Chinese medicine. The cavitation effect generated by ultrasound can destroy the cell structure of traditional Chinese medicine, making the effective ingredients of traditional Chinese medicine easier to dissolve. Adding cellulase and polyglycerol fatty acid esters to traditional Chinese medicine during ultrasonic equipment treatment can more effectively promote the dissolution of traditional Chinese medicine ingredients, increase the active ingredients such as total flavonoids, polysaccharides, gardenia glycosides and alkaloids in traditional Chinese medicine compositions, and enhance the efficacy.

[0069] The liver injury property of the Chinese medicine compositions prepared in Examples 1 and 2 and Comparative Examples 1, 2 and 3 was determined.

[0070] Healthy mice were divided into 6 groups, each group of male and female mice had 10 each, and each group of mice was fed with the Chinese medicine composition prepared by Example 1, 2 and Comparative Examples 1, 2 and 3 every 12h, and the feeding dose was 0.2g / kg, and the control group (CK group) was a non-medication group. After continuous feeding for 7 days, after the last feeding for 2h, a one-time intraperitoneal injection of an olive oil solution with a concentration of 0.2%CCl4 was performed, and blood was taken after fasting for 24h, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values ​​were measured, and liver tissue was taken, and superoxide dismutase (SOD) content was measured after treatment, and the results are shown in Table 2.

[0071] Table 2: Analysis of CCl4 liver injury results

[0072] AST(U / L) ALT(U / L) SOD (U / ml) Embodiment 1 83.23 76.54 2.98 Embodiment 2 79.86 80.12 2.86 Comparative Example 1 105.46 98.62 2.16 Comparative Example 2 98.65 95.34 2.45 Comparative Example 3 100.27 88.76 2.73 CK group 195.45 183.92 1.12

[0073] As shown in Table 2, the mice fed with the Chinese medicine compositions prepared in Examples 1 and 2 had lower AST and ALT contents and higher SOD contents. The AST content in Example 1 was 21.1%, 15.6%, 17.0% and 57.4% lower than those in Comparative Examples 1, 2, 3 and CK group, respectively; the ALT content in Example 1 was 22.4%, 19.7%, 13.8% and 58.4% lower than those in Comparative Examples 1, 2, 3 and CK group, respectively; and the SOD content in Example 1 was 27.5%, 17.8%, 8.4% and 62.4% higher than those in Comparative Examples 1, 2, 3 and CK group, respectively.

[0074] AST plays a key role in amino acid metabolism. AST can catalyze the transamination reaction between oxaloacetic acid and glutamic acid, which is conducive to the normal operation of liver metabolism. ALT participates in the protein metabolism process in the liver and can convert amino acids into urea and other metabolites. When liver cells are damaged, AST and ALT will be released into the blood, resulting in increased AST and ALT levels in the blood. The serum AST and ALT in Example 1 and Example 2 are relatively low, indicating that Example 1 and Example 2 are effective in reducing AST and ALT levels in serum, and the degree of liver damage is relatively small. SOD is an antioxidant enzyme that can effectively remove free radicals in the human body and reduce the damage of free radicals to cells, thereby delaying aging and preventing diseases. When liver cells are damaged, the amount of free radicals produced in the body exceeds the antioxidant capacity of the body, resulting in the consumption or inactivation of antioxidant enzymes such as SOD. In Example 1 and Example 2, SOD activity is relatively high, and the effect is better in enhancing antioxidant. In the preparation process of the Chinese medicine composition, adding cellulase and polyglycerol fatty acid esters and using ultrasonic equipment to treat can improve the extraction effect of the active ingredients of Chinese medicine, enhance the efficacy, thereby reducing liver damage and enhancing antioxidant capacity.

[0075] In summary, the Chinese medicine composition prepared by the present invention has a high content of effective ingredients, strong medicinal efficacy, and good taste, and can solve liver conditioning problems such as liver protection and liver protection. The Chinese medicines used are all medicinal and edible substances, have no side effects on the human body, and can be taken for a long time.

[0076] The above are only specific embodiments of the present invention, but the technical features of the present invention are not limited thereto. Any simple changes, equivalent replacements or modifications made based on the present invention to solve basically the same technical problems and achieve basically the same technical effects are all included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition for liver conditioning, characterized in that: The preparation method is prepared by boiling and concentrating the following components by weight: 68-75 parts of Chinese medicine decoction, 6-8 parts of erythritol, 4-5 parts of nisin, and 3-4 parts of concentrated lemon juice; The Chinese medicine decoction is prepared by adding water to the following components in parts by weight, ultrasonically treating the components, and then decocting the components: 35-38 parts of bergamot, 30-32 parts of gardenia, 25-28 parts of mulberry leaves, 22-25 parts of chrysanthemum, 18-20 parts of lotus leaves, 14-16 parts of mulberry, 10-12 parts of polygonatum, 8-10 parts of wolfberry, 5-6 parts of hawthorn, 3-5 parts of tangerine peel, 3-4 parts of spiny jujube kernel, 2-4 parts of momordica grosvenori, 1.5-3 parts of polyglycerol fatty acid esters, and 1-2 parts of cellulase.

2. The Chinese medicine composition for liver conditioning according to claim 1, characterized in that: The Chinese medicine composition for liver conditioning is prepared by the following preparation method: S1. Chinese medicine crushing: bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel, and monk fruit are crushed in a grinder respectively, so that the particle size of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel, and monk fruit is less than or equal to 1.2mm; S2. Ultrasonic treatment of Chinese medicine: add water, polyglycerol fatty acid ester, and cellulase in an ultrasonic device in order and mix them evenly, add an acid-base regulator to adjust the pH value to 6.0-6.5, and then add crushed bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube kernel, and monk fruit in proportion, and ultrasonically treat for 30-35 minutes to obtain a solid-liquid mixture of Chinese medicine; S3. Chinese medicine decoction: Place the Chinese medicine solid-liquid mixture in an electric heating sandwich pot, boil over high heat, then turn to simmer for 50 to 60 minutes, filter to obtain a decoction, add water to the Chinese medicine residue after the first decoction, boil over high heat, then turn to simmer for 50 to 60 minutes, filter to obtain a second decoction, and mix the two decoctions to obtain a Chinese medicine decoction; S4. Preparation of Chinese medicine: Place the Chinese medicine decoction into an electric heating double-layer pot, add erythritol, nisin, and concentrated lemon juice, and simmer over low heat until concentrated by 2 to 2.5 times to obtain a Chinese medicine composition.

3. The Chinese medicine composition for liver conditioning according to claim 2, characterized in that: Before crushing bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, wild jujube kernel and momordica grosvenori, the method further comprises: drying the bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, wild jujube kernel and momordica grosvenori at 50-55° C. to make the moisture content of the bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, wild jujube kernel and momordica grosvenori reach 7-9%.

4. The Chinese medicine composition for liver conditioning according to claim 2, characterized in that: The temperature of the ultrasonic equipment is set to 37-42° C. and the frequency is set to 110-115 Hz.

5. The Chinese medicine composition for liver conditioning according to claim 2, wherein the acid-base regulator is at least one of citric acid and tartaric acid.

6. The Chinese medicine composition for liver conditioning according to claim 2, characterized in that: The cellulase activity is 30-35 U / mg.

7. The Chinese medicine composition for liver conditioning according to claim 2, characterized in that: During the ultrasonic treatment of the traditional Chinese medicine, the amount of water added is 4 to 5 times the total weight of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, jujube seed and monk fruit.

8. The Chinese medicine composition for liver conditioning according to claim 2, characterized in that: During the secondary decoction of the traditional Chinese medicine, the amount of water added is 3 to 4 times the total weight of bergamot, gardenia, mulberry leaf, chrysanthemum, lotus leaf, mulberry, polygonatum, wolfberry, hawthorn, tangerine peel, spiny jujube kernel and monk fruit.

9. The Chinese medicine composition for liver conditioning according to claim 1 or 2, characterized in that: The concentrated lemon juice has a concentration degree of 5 to 6 times.

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