Traditional Chinese medicine composition for treating candida diseases

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for treating candida diseases. The composition mainly comprises a component A and a component B, and the mass ratio of the component A to the component B is (1-20): 1. Wherein the component A is curcuma zedoary volatile oil, and the component B is peppermint oil or menthol. The composition acts on the oral cavity, the formula has a relatively strong inhibition effect on candida albicans, and the composition has fresh and sweet mouth feel while having oral cavity cooling feeling, and is long in oral cavity cooling feeling duration, good in sense and free of irritation to oral mucosa. The curcuma zedoary volatile oil and the dementholized peppermint oil cooperate to inhibit candida albicans, and meanwhile, the mouth mucosa irritation can be reduced to a certain extent.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicine compositions, and specifically relates to a Chinese medicine composition for treating candidiasis. Background Art

[0002] Oral thrush, also known as oral candidiasis, is a fungal infection caused by Candida species (usually Candida albicans). It commonly affects infants and young children, older adults, and people with compromised immune systems. Here are some of the medications commonly used to treat thrush and their possible pros and cons:

[0003] Fluconazole: Fluconazole is an oral antifungal that is commonly used to treat various infections caused by Candida, including thrush. It acts on the cell membrane of the fungal cells, preventing the fungus from growing. Possible side effects include nausea, diarrhea, rash, and, with long-term use, possible liver problems. In addition, some strains of Candida are resistant to fluconazole.

[0004] Itraconazole: Similar to fluconazole, itraconazole is a broad-spectrum antifungal that can be taken orally or injected. Side effects may include headache, nausea, high potassium levels, and irregular heartbeats. It is not recommended for patients with heart problems.

[0005] Amphotericin B: It is a powerful antifungal drug, commonly used for severe or refractory fungal infections. This drug may cause a variety of side effects, including fever, chills, kidney damage, vomiting, hypokalemia, etc. It is generally only used when other treatments fail.

[0006] Nystatin: This is a commonly used topical antifungal for the treatment of oral candidiasis. Nystatin comes in the form of a suspension that the patient needs to swish in the mouth and then swallow or spit out. Although it has fewer side effects, it can be inconvenient to use and requires multiple rinsings. It may sometimes be less effective than systemic antifungals.

[0007] Clotrimazole: This is a topical antifungal that usually comes in lozenge form to treat thrush. Possible side effects include stinging and swelling in the mouth. It may not be effective enough for severe infections. Summary of the invention

[0008] In order to solve the problems existing in the prior art, the present invention aims at a Chinese medicine composition for treating candidal diseases.

[0009] To achieve the above purpose, the present invention adopts the following technical solution:

[0010] A traditional Chinese medicine composition for treating candidiasis, wherein the main components of the composition are component A and component B, wherein the mass ratio of component A to component B is 1-20:1; wherein component A is volatile oil of zedoaria zedoaria, and component B is peppermint oil or menthol.

[0011] The mass ratio of component A to component B is 1-10:1.

[0012] The component A is volatile oil of Curcuma zedoaria, the component B is peppermint oil, and the mass ratio of the component A to the component B is 1-10:1.

[0013] The volatile oil of Curcuma zedoaria is obtained by solvent extraction from Curcuma zedoaria, or can be obtained commercially.

[0014] The volatile oil of zedoary turmeric contains, by weight percentage, 7.50%-15.83% of curdione, 10.00%-20.83% of curcumol, 10.00%-20.83% of germacronone, 10.00%-15.83% of curcumene, 10.00%-15.83% of furadiene and 5.00%-10.83% of beta-elemene.

[0015] The composition comprises curcumadione, curcumol, germacrone, curcumene, furadiene, β-elemene and component B, wherein the total amount of curcumadione, curcumol, germacrone, curcumene, furadiene and β-elemene and component B is 1-10:1.

[0016] The composition further contains lecithin and chitosan, wherein the lecithin accounts for 0.3-0.9% by weight of the composition according to claim 1, and the chitosan accounts for 0.03-0.09% by weight of the composition according to claim 1.

[0017] An application of the traditional Chinese medicine composition for treating candidal diseases, and an application of the composition in treating candidal diseases.

[0018] An application of the traditional Chinese medicine composition for treating candidal diseases, and an application of the composition in preparing a medicine for treating candidal diseases.

[0019] Beneficial effects of the present invention:

[0020] The composition of the present invention acts on the oral cavity, and the formula has a strong inhibitory effect on Candida albicans, and the mouthfeel is good. It has a refreshing and sweet taste while having a refreshing feeling in the oral cavity, and the refreshing feeling in the oral cavity lasts for a long time, and the sense of sense is good, and there is no irritation to the oral mucosa; the present invention utilizes the volatile oil of Curcuma zedoariae and the peppermint oil to cooperate with each other to synergistically inhibit Candida albicans, and can reduce the irritation of the oral mucosa to a certain extent. It is safe and reliable to use, has no toxic side effects, has a significant effect on the treatment of thrush, and has a short treatment time, has the effects of promoting blood circulation, relieving pain, promoting myogenesis and anti-inflammatory, detoxifying, and astringing. The present invention has a good therapeutic effect on thrush, and has the characteristics of high efficacy, no recurrence, and convenient carrying, and is beneficial to industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The HPLC chromatogram of the main components of the zedoary turmeric oil provided in the embodiment of the present invention.

[0022] Figure 2 This is a diagram showing the antibacterial effects of different proportions of Curcuma volatile oil provided in an embodiment of the present invention.

[0023] Figure 3 This is a diagram showing the antibacterial effects of different proportions of Curcuma volatile oil and peppermint oil provided in an embodiment of the present invention.

[0024] Figure 4 This is a diagram showing the antibacterial effects of chitosan at different concentrations provided in an embodiment of the present invention.

[0025] Figure 5 This is a diagram showing the antibacterial effects of lecithin at different concentrations provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0026] The specific implementation modes of the present invention are further described below in conjunction with examples. It should be noted that the specific implementation modes described here are only for illustrating and explaining the present invention, and are not limited to the present invention.

[0027] Example 1

[0028] Extraction of volatile oil from Curcuma zedoaria

[0029] The invention adopts ultrasonic pretreatment-microwave method to extract zedoary turmeric oil. Take dried zedoary turmeric slices, crush them, pass through a 50-mesh sieve to obtain zedoary turmeric powder, add them into excess ethanol, and perform ultrasonic treatment for 30 minutes; remove 70% of organic solvent by vacuum distillation; add excess water, and perform 200W microwave extraction; after the extraction is complete, collect the oil phase, add a conventional desiccant to dry, and remove the remaining organic solvent by vacuum distillation to obtain zedoary turmeric volatile oil.

[0030] The powder of medicinal materials was treated with ultrasonic wave by organic solvent, and physical crushing was achieved during the ultrasonic treatment process, so that most of the volatile oil was dissolved in the solvent, and then the organic solvent was removed to obtain the volatile oil. The yield was measured to be 3.5% to 4.7%. The volatile oil extracted by HPLC method contained 6 effective components, including curcuma dione, curcuma alcohol, germacrone, curcumene, furadiene, and β-elemene. The results are as follows Figure 1 As shown, this method has the characteristics of low cost and high efficiency.

[0031] Meanwhile, the volatile oil of Curcuma zedoaria used in the following examples can also be obtained through commercial means.

[0032] Example 2

[0033] Antibacterial activity of volatile oil from Curcuma zedoaria

[0034] The present embodiment adopts the zedoary turmeric volatile oil (also commercially available) prepared by embodiment 1, and verifies the antibacterial ability of zedoary turmeric volatile oil at the same time, and carries out the dilution gradient experiment of zedoary turmeric volatile oil, first dissolves zedoary turmeric volatile oil in Tween 80, then dilutes with sterile water, and uses Tween 80 as blank control, and zedoary turmeric volatile oil is prepared to obtain different mass concentrations of 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.2%. Use the pre-add bacteria pouring plate method, the specific operation method is to pour the Candida albicans suspension (that is, the Candida albicans grown to the logarithmic growth phase is prepared into 0.5 McFarland concentration) into the beef extract protein solid culture medium cooled to room temperature in advance, and evenly spread it on the culture surface, and obtain the culture dish of Candida albicans, standby. At the same time, use the blank without adding zedoary turmeric volatile oil.

[0035] The Oxford cup method was used to examine the antibacterial effect: the Oxford cup was placed on the surface of the culture dish, 100 μL of the drug solution of different concentrations was added to the Oxford cup, and the solution was cultured in a constant temperature environment of 37°C for 24 hours. Observation results: The larger the diameter of the antibacterial circle, the greater the antibacterial effect of the essential oil, and the antibacterial rate was calculated at the same time.

[0036] Inhibition rate = (inhibition zone diameter of treatment group - inhibition zone diameter of blank group) / inhibition zone diameter of treatment group × 100%

[0037] The inhibition zones of different concentrations of volatile oil from Curcuma zedoariae are as follows Figure 2 As shown in Table 1, the antibacterial effect of the volatile oil of Curcuma zedoaria is shown to be strong in antibacterial effect, and the antibacterial rate of 0.1% volatile oil of Curcuma zedoaria can reach 65.63%. Since the volatile oil of Curcuma zedoaria has good antibacterial, anti-inflammatory and antibacterial effects, it can inhibit oral bacteria, and it also has the characteristics of Chinese herbal medicine that it is not easy to produce drug resistance. After being made into an oral antibacterial product, it can be used for a long time without side effects.

[0038] Table 1 Antibacterial effect of different concentrations of volatile oil of Curcuma zedoaria

[0039]

[0040]

[0041] Example 3

[0042] Synergistic antibacterial effect of volatile oil of Curcuma zedoaria and peppermint oil

[0043] In order to enhance the antibacterial ability of zedoary volatile oil, this embodiment uses zedoary volatile oil and peppermint oil for compounding, and studies the antibacterial effect of the composition. Zedoary volatile oil and peppermint oil are dissolved in Tween 80 to prepare compositions with different mass ratios of 20:1, 10:1, 1:1, 1:10, and 1:20, and the composition without addition is used as a blank. The specific antibacterial experimental method is the same as above, and the amounts used in the experiment are the same.

[0044] The antibacterial zones of volatile oil of Curcuma zedoaria and peppermint oil with different mass ratios were as follows Figure 3 The antibacterial effect is shown in Table 2, which shows that the combination of Curcuma volatile oil and peppermint oil significantly improves the antibacterial effect, among which the antibacterial effect of the combination of Curcuma volatile oil and peppermint oil with a mass ratio of 10:1 is the best, and the antibacterial rate is 71.76%, indicating that the combination of Curcuma volatile oil and peppermint oil in a specific mass ratio has a synergistic effect.

[0045] Table 2 Results of antibacterial activity of mixed oil phases in different proportions

[0046]

[0047] Example 4

[0048] Antibacterial effect of chitosan or lecithin at different concentrations

[0049] Chitosan or lecithin is added to the composition obtained in the above Example 3 for compounding research, that is, the composition is mixed and prepared in the manner described in Example 3, and the mass ratio of zedoary volatile oil to peppermint oil in the composition is 10:1;

[0050] Different amounts of chitosan were added to the above composition, so that the mass concentration of chitosan in the composition was 0.03%, 0.06% and 0.09% respectively;

[0051] Different amounts of lecithin were added to the above composition, so that the mass concentration of lecithin in the composition was 0.3%, 0.6% and 0.9% respectively;

[0052] Then, the above-mentioned compositions with different chitosan or lecithin added were used to conduct an inhibition zone experiment, while the composition without addition was used as a blank. The specific antibacterial experimental method was the same as above, and the amounts used in the experiment were the same. The culture medium was placed in an incubator and cultured for 24 hours. The antibacterial effects were compared by comparing the sizes of the inhibition zones.

[0053] The inhibition zone of chitosan with different concentrations was Figure 4 The antibacterial effect is shown in Table 3; the antibacterial zones of lecithin with different concentrations are shown in Table 3. Figure 5 The antibacterial effects are shown in Table 4.

[0054] Table 3 Antibacterial effect of chitosan at different concentrations

[0055]

[0056] Table 4 Antibacterial effect of lecithin at different concentrations

[0057]

[0058] The above results show that with the increase of chitosan concentration, the antibacterial effect shows a trend of first increasing and then weakening, and the antibacterial effect is best when the chitosan concentration is 0.06%; with the increase of lecithin concentration, the antibacterial effect shows an increasing trend, and the antibacterial effect is best when the lecithin concentration is 0.6%. Therefore, it is shown that the use of specific concentrations of chitosan or lecithin can synergistically cooperate with the composition to show a better antibacterial effect; chitosan or lecithin affects the ionic microenvironment related to the survival of microorganisms, and the constructed nanodrug delivery system can improve the permeability of bacterial cell walls, thereby playing an antibacterial role.

[0059] Example 5

[0060] Antibacterial effect of chitosan and lecithin added simultaneously to the composition

[0061] It can be seen from Table 3 and Table 4 that chitosan and lecithin can promote the composition to have a certain antibacterial effect when used alone. It can be inferred that adding the two to the composition can have a synergistic antibacterial effect.

[0062] Therefore, chitosan and lecithin were added to the composition obtained in the above Example 3 for compounding research, that is, the composition was mixed and prepared in the manner described in Example 3, and the mass ratio of zedoary volatile oil to peppermint oil in the composition was 10:1;

[0063] 0.02% chitosan, 0.6% lecithin, 0.02% chitosan and 0.6% lecithin, and 0.06% chitosan and 0.6% lecithin were respectively added to the composition to prepare compound Chinese medicine compositions as experimental groups, while the composition without addition was used as blank and the existing drug was used as control. The specific antibacterial zone experiment was carried out in the same manner as above. The amounts used in the experiment were the same. The culture medium was placed in an incubator and cultured for 24 hours. The antibacterial effects were compared by comparing the sizes of the inhibition zones.

[0064] The synergistic antibacterial effect of chitosan and lecithin is shown in Table 5. The results show that after chitosan lecithin is compounded with the traditional Chinese medicine composition, the rate of inhibition zone is significantly higher than that of adding them separately, indicating that the mixture of the two has obvious synergistic effect and can significantly enhance the antibacterial effect, which provides theoretical and technical support for the development and utilization of subsequent compositions.

[0065] Table 5 Synergistic antibacterial effect of chitosan and lecithin

[0066]

[0067] The present application describes multiple embodiments, but the description is exemplary rather than restrictive, and it is obvious to a person skilled in the art that there may be more embodiments and implementations within the scope of the embodiments described in the present application.

Claims

1. A Chinese medicine composition for treating candidal diseases, characterized in that: The main components of the composition are component A and component B, and the mass ratio of component A to component B is 1-20:1; wherein component A is volatile oil of zedoary turmeric, and component B is peppermint oil or menthol.

2. The Chinese medicine composition for treating candidal diseases according to claim 1, characterized in that: The mass ratio of component A to component B is 1-10:

1.

3. The Chinese medicine composition for treating candidal diseases according to claim 1 or 2, characterized in that: The component A is volatile oil of Curcuma zedoaria, the component B is peppermint oil, and the mass ratio of the component A to the component B is 1-10:

1.

4. The Chinese medicine composition for treating candidal diseases according to claim 1, characterized in that: The volatile oil of zedoaria is obtained by extracting zedoaria from zedoaria through solvent.

5. The Chinese medicine composition for treating candidal diseases according to claim 4, characterized in that: The volatile oil of zedoary turmeric contains, by weight percentage, 7.50%-15.83% of curdione, 10.00%-20.83% of curcumol, 10.00%-20.83% of germacronone, 10.00%-15.83% of curcumene, 10.00%-15.83% of furadiene and 5.00%-10.83% of beta-elemene.

6. The Chinese medicine composition for treating candidal diseases according to claim 4 or 5, characterized in that: The composition comprises curcumadione, curcumol, germacrone, curcumene, furadiene, β-elemene and component B, wherein the total amount of curcumadione, curcumol, germacrone, curcumene, furadiene and β-elemene and component B is 1:1-10.

7. The Chinese medicine composition for treating candidal diseases according to any one of claims 1 to 6, characterized in that: The composition further contains lecithin and chitosan, wherein the lecithin accounts for 0.3-0.9% by weight of the composition according to claim 1, and the chitosan accounts for 0.03-0.09% by weight of the composition according to claim 1.

8. Use of the Chinese medicine composition for treating candidal diseases according to claim 1, characterized in that: The application of the composition in treating candidal diseases.

9. Use of the Chinese medicine composition for treating candidal diseases according to claim 1, characterized in that: The composition is used in preparing medicine for treating candidiasis.

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