Antibody preparation device and antibody preparation process

Abstract

The invention discloses an antibody preparation device and an antibody preparation process, and relates to the field of antibody preparation equipment.The antibody preparation device comprises a shell, an air inlet pipe, an air outlet pipe and a feeding pipe, a fan is rotationally arranged outside the shell, and a speed adjusting mechanism used for adjusting the rotating speed of the fan is further arranged outside the shell; a pressure sensing diaphragm is arranged on the outer side wall of the shell, ball valves are installed in the air inlet pipe and on the air outlet pipe, and a driving mechanism used for driving valve bodies in the ball valves to rotate is arranged outside the shell. According to the present invention, the temperature and the pressure in the housing can be conveniently controlled so as to reduce the influence on the antibody production.

Description

Technical Field

[0001] The present application relates to the field of antibody preparation equipment, and in particular to an antibody preparation device and an antibody preparation process. Background Art

[0002] Antibody preparation is one of the key technologies in the field of biotechnology and pharmaceuticals, and is widely used in disease diagnosis, treatment and research. Antibody preparation involves a variety of materials, such as antigens, adjuvants, culture media, etc. Among them, mixing equipment, as the main device for antibody preparation, plays a vital role. In the process of antibody preparation, the stirring action of the mixing device promotes the chemical reaction between materials, accelerates the generation of antibodies, and ensures the preparation effect of antibodies.

[0003] In the prior art, an antibody mixing device includes a shell, a stirring rod is rotatably arranged inside the shell for stirring and mixing the materials in the shell, a feed pipe and a discharge pipe are connected to the outside of the shell, and an air inlet pipe and an air outlet pipe are also connected to the outside of the shell; however, when preparing antibodies, the mixing reaction between the materials may be accompanied by an exothermic reaction. In addition, when the stirring device is running at a high speed, friction between mechanical parts generates heat, and long-term operation may cause heat accumulation, thereby causing the temperature in the equipment to rise. At the same time, the temperature increase will accelerate the movement of liquid molecules, resulting in an increase in vapor pressure and an increase in the pressure in the shell; excessive temperature and pressure not only reduce the activity of antibody proteins but also affect cell growth and metabolism, thereby reducing the production efficiency of antibodies and increasing production costs. Summary of the invention

[0004] In order to facilitate the control of the temperature and pressure inside the shell, thereby reducing the impact on antibody production, the present application provides an antibody preparation device and an antibody preparation process.

[0005] The present application provides an antibody preparation device using the following technical solution: An antibody preparation device comprises a shell, an air inlet pipe, an air outlet pipe, a feed pipe and a stirring rod, wherein a fan is rotatably arranged outside the shell, and a speed regulating mechanism for adjusting the fan speed is also arranged outside the shell; a pressure sensing diaphragm is arranged on the outer side wall of the shell, ball valves are installed in the air inlet pipe and on the air outlet pipe, and a driving mechanism for driving a valve body in the ball valve to rotate is arranged outside the shell.

[0006] By adopting the above technical scheme, when the materials are mixed and stirred in the shell, the stirring rod can rotate and the fan can be driven to rotate through the speed regulating mechanism at the same time. The rotation of the fan can cool the inner cavity of the shell. If the temperature in the shell gradually becomes too high, the speed regulating mechanism is triggered at this time. The speed regulating mechanism can adjust the speed of the fan to increase the fan speed, thereby accelerating the reduction of the temperature in the inner cavity of the shell; the set pressure sensing diaphragm can monitor the pressure changes inside the shell in real time. When the pressure in the shell gradually increases, the pressure sensing diaphragm can be deformed and trigger the driving mechanism. The driving mechanism can adjust the opening and closing of the ball valve, so that the ball valve in the air inlet pipe is gradually closed, and the ball valve in the air outlet pipe is gradually opened, ensuring the pressure stability during the mixing process; the whole process ensures that the temperature and pressure of the inner cavity of the shell are in a state suitable for antibody production, thereby improving the mixing efficiency of the materials and the quality of the antibody products.

[0007] Preferably, the speed regulating mechanism comprises a rotating rod 1, a rotating rod 2 and a rotating rod 3, the rotating rod 1 is vertically rotatably arranged outside the shell, a bevel gear 1 is fixedly arranged on the fan shaft, a bevel gear 2 is fixedly arranged on the rotating rod 1, and the bevel gear 2 is meshed with the bevel gear 1; the rotating rod 2 is horizontally rotatably arranged outside the shell, a bevel gear 3 is fixedly arranged on the rotating rod 1, a bevel gear 4 is fixedly arranged on the rotating rod 2, and the bevel gear 4 is meshed with the bevel gear 3; a bevel gear 5 is fixedly arranged on the rotating rod 3, one end of the stirring rod extends to the outside of the shell and is fixedly arranged with a bevel gear 6, and the bevel gear 6 is meshed with the bevel gear 5; The central axis of the rotating rod 2 is parallel to the central axis of the rotating rod 3, and a plurality of gears 1 with different diameters are rotatably arranged on the rotating rod 2, and the plurality of gears 1 are arranged in sequence along the central axis of the rotating rod 2 according to their diameters; a plurality of gears 2 with different diameters are fixedly arranged on the rotating rod 3, and the plurality of gears 2 are arranged in sequence along the central axis of the rotating rod 3 according to their diameters, and the diameter change trends of the plurality of gears 1 and the plurality of gears 2 in the same direction are opposite, and the plurality of gears 1 are meshed with the plurality of gears 2 in a one-to-one correspondence; a plurality of positioning blocks are slidingly arranged on the inner wall of the rotating rod 2 corresponding to each of the gears 1, and a positioning groove for the positioning block to be inserted is opened on the inner wall of the gear 1, and the speed regulating mechanism also includes a first driving component for driving the positioning block to move.

[0008] By adopting the above technical solution, at the initial stage of mixing and stirring the materials, the positioning block corresponding to the larger diameter gear 1 is inserted into the positioning groove in the larger diameter gear 1 under the drive of the first driving component. At this time, the larger diameter gear 1 is fixed to the rotating rod 2, the stirring rod drives the bevel gear 6 to rotate, the bevel gear 6 drives the bevel gear 5 and the rotating rod 3 to rotate, the rotating rod 3 drives multiple gear 2 to rotate, when the larger diameter gear 1 is fixed to the rotating rod 2, the corresponding gear 2 can drive it to rotate, the larger diameter gear 1 drives the rotating rod 2 to rotate, the rotating rod 2 drives the bevel gear 4 to rotate, the bevel gear 4 drives the bevel gear 3 and the rotating rod 1 to rotate, the rotating rod 1 drives the bevel gear 2 to rotate, and the bevel gear The second gear drives the bevel gear one and the shaft of the fan to rotate. At this time, the speed of the fan is relatively low. When the temperature in the shell gradually becomes too high, the first drive component is triggered. The first drive component drives the positioning block corresponding to the smaller diameter gear one to be inserted into the positioning groove of the corresponding gear one. The smaller diameter gear one is fixed to the rotating rod two, and the larger diameter gear one is separated from the corresponding positioning block. At this time, the speed of the fan increases. When the temperature in the shell continues to rise, the first drive component can fix the smaller diameter gear one to the rotating rod two, thereby further increasing the speed of the fan. The whole process facilitates precise speed regulation of the fan, thereby facilitating temperature regulation in the shell.

[0009] Preferably, the first driving assembly includes a bimetallic strip, a driving rod 1, a driving rod 2 and a driving rod 3, the bimetallic strip being installed in the shell, the driving rod 1 sliding in the shell, and one end of the driving rod 1 abutting against the bimetallic strip, the other end of the driving rod 1 passing through the shell, the driving rod 3 sliding in the rotating rod 2, the two ends of the driving rod 2 being hinged to the driving rod 1 and the driving rod 3 respectively, a plurality of top blocks being rotatably arranged on the driving rod 3, a plurality of the top blocks being arranged correspondingly to a plurality of the positioning blocks, inclined surfaces being arranged on opposite sides of the top blocks, the top blocks being slidingly matched with the top blocks through the inclined surfaces on both sides thereof, and each of the top blocks being matched asynchronously with each of the positioning blocks; the rotating rod 2 is also provided with a resetting member 1 for resetting the positioning block, and a resetting member 2 for resetting the driving rod 1 is also provided in the shell.

[0010] By adopting the above technical solution, when the temperature in the shell increases, the bimetallic strip is deformed when the temperature changes, thereby pushing the driving rod 1 to move, the driving rod 1 drives the driving rod 2 to rotate, the driving rod 2 drives the driving rod 3 to move, and the driving rod 3 simultaneously drives multiple top blocks to move. At this time, the top block corresponding to the smaller diameter gear 1 can push the corresponding positioning block through its inclined surface to be inserted into the positioning groove on the corresponding gear 1, so that the gear 1 is fixed to the rotating rod 2, and at the same time, the top block corresponding to the larger diameter gear 1 is away from the corresponding positioning block. The positioning block can be away from the corresponding gear 1 under the action of the reset member 1, and so on. When the temperature in the shell gradually increases, the bimetallic strip can gradually fix the smaller diameter gear 1 to the rotating rod 2, so that the speed of the fan gradually increases, and the reset member 2 provided can reset the driving rod 1.

[0011] Preferably, the driving mechanism includes a rack, a gear three and a driving rod four, the rack slides outside the shell, one end of the rack abuts against the outer wall of the pressure sensing diaphragm, the two ends of the driving rod four are respectively fixed to the rotating shaft of the valve body inside the ball valve in the intake pipe and the rotating shaft of the valve body inside the ball valve in the outlet pipe, the gear three is fixed on the driving rod four, and the rack is meshed with the gear three, and a reset member three for resetting the rack is provided outside the shell.

[0012] By adopting the above technical solution, the driving mechanism can automatically adjust the opening and closing of the ball valve according to the pressure changes inside the shell. When the pressure in the shell gradually increases, the pressure sensing diaphragm is deformed and pushes the rack to move, and then the driving rod four is driven to rotate through the gear three, so that the opening and closing degree of the ball valve in the air inlet pipe is reduced, and the opening and closing degree of the ball valve in the air outlet pipe is increased, so that the equipment can automatically adjust the air intake and air outlet according to the internal pressure, ensure the pressure stability during the mixing process, and improve the automation and reliability of the equipment.

[0013] Preferably, a condensation box for storing condensed liquid is arranged outside the shell, and a heat sink is installed on the outer side wall of the condensation box, a channel for the circulation of condensed liquid is opened in the stirring rod, a connecting pipe 1 and a connecting pipe 2 are rotatably arranged on the stirring rod, and the side walls of the condensation box are respectively connected with a liquid inlet pipe and a liquid outlet pipe; the connecting pipe 1 is respectively connected to the outlet of the channel in the stirring rod and the liquid inlet pipe, and the connecting pipe 2 is respectively connected to the inlet of the channel in the stirring rod and the liquid outlet pipe, and a one-way valve is arranged in the liquid inlet pipe and the liquid outlet pipe; a conveying component for conveying condensed liquid is arranged outside the shell.

[0014] By adopting the above technical solution, the rotating rod 2 can trigger the conveying component while rotating, and the conveying component can make the condensed liquid circulate through the channel in the stirring rod to take away the heat and ensure the stability of the reaction temperature; the condensation box can effectively take away the heat generated in the mixing process, and the heat sink provided further improves the heat dissipation effect of the condensation box to maintain the stability of the reaction temperature; when the condensed liquid flows in the stirring rod, it exchanges heat with the material outside the stirring rod, and can quickly absorb the heat of the material, thereby reducing the temperature of the material. This direct heat exchange method further improves the cooling efficiency of the equipment.

[0015] Preferably, the conveying assembly includes a piston rod, a rotating plate and a connecting rod 1, one end of the piston rod is slidably connected to the inner wall of the condensation box, the rotating plate is fixedly arranged on the rotating rod 2, one end of the connecting rod 1 is rotatably arranged on the rotating plate, and the other end of the connecting rod 1 is hinged to the piston rod.

[0016] By adopting the above technical solution, the rotating rod 2 can drive the rotating plate to rotate while rotating, the rotating plate drives the connecting rod 1 to rotate, and the connecting rod 1 drives the piston to reciprocate, thereby realizing the transportation of the condensed liquid. At the same time, when the temperature of the equipment gradually increases, the rotation speed of the rotating rod 2 increases, so the movement rate of the piston increases. As the temperature rises, the condensed liquid absorbs more heat in the stirring rod, the piston moves faster, and the circulation of the condensed liquid is accelerated, and a faster heat exchange is carried out with the material outside the stirring rod, thereby more effectively reducing the material temperature and further improving the efficiency of the heat exchange.

[0017] Preferably, a partition is fixedly provided on the inner wall of the feed pipe, and the partition divides the inner cavity of the feed pipe into a grinding cavity and a conveying cavity connected to the inner cavity of the shell. A through hole is opened on the partition, and a sieve plate is installed in the through hole. A slide plate is vertically slid in the grinding cavity, and a grinding plate is provided on the side of the slide plate close to the sieve plate. A second driving component for driving the grinding plate to move vertically is provided outside the shell.

[0018] By adopting the above technical solution, the material first enters the grinding chamber, and the stirring rod can trigger the second drive assembly while rotating. The second drive assembly can drive the grinding plate to move repeatedly in the vertical direction. The grinding plate can grind and crush the material, thereby pre-crushing and screening the material to ensure that the material has a uniform particle size. The setting of the screen plate can screen the material to prevent large particles from entering the mixing chamber. Grinding and crushing can significantly increase the specific surface area of ​​the material, so that the material can be more fully in contact with the reaction medium or enzyme, thereby improving the reaction rate and efficiency; grinding and crushing can make materials of different components mix more evenly, which helps to improve the uniformity and consistency of the reaction.

[0019] Preferably, the grinding plate slides horizontally on a side of the slide plate close to the screen plate, and a third driving component for driving the grinding plate to move horizontally is provided in the feed pipe.

[0020] By adopting the above technical solution, during the vertical movement of the grinding plate, the third driving assembly is triggered, so that the grinding plate moves repeatedly in the horizontal direction at the same time. The horizontal movement of the grinding plate can further grind the material, ensure the uniform particle size of the material, improve the grinding effect, and ensure the uniformity of the material during the mixing process.

[0021] Preferably, the sieve plate slides horizontally on the partition plate, and a fourth driving component for driving the sieve plate to move horizontally is provided outside the shell.

[0022] By adopting the above technical solution, the horizontal movement of the screen plate can realize the screening of materials and prevent large particles from entering the mixing chamber. While the grinding plate is grinding, the fourth drive assembly is triggered, and the fourth drive assembly can simultaneously drive the screen plate to move repeatedly in the horizontal direction, thereby accelerating the ground material to fall into the conveying chamber and reducing the possibility of material blockage above the screen plate.

[0023] A process for preparing an antibody comprises the following steps: S1. Material preparation and feeding: Add materials such as antigens and adjuvants into the shell through the feed pipe. The grinding chamber in the feed pipe pre-crushes and screens the materials through the grinding plate and the sieve plate. The grinding plate moves vertically through the second drive assembly, and the sieve plate moves horizontally through the fourth drive assembly to ensure uniform particle size of the materials and avoid large particles affecting the mixing effect; S2, mixing and stirring: start the stirring rod, which can stir and mix the materials in the shell. Under the stirring of the stirring rod, the materials are fully mixed in the shell to form a uniform mixture; S3, temperature and pressure regulation: The pressure sensing diaphragm outside the shell monitors the internal pressure in real time, and adjusts the ball valves in the air inlet and outlet pipes through the driving mechanism to achieve precise pressure control; the bimetallic strip controls the movement of the positioning block through the driving rods 1, 2 and 3, adjusts the meshing of gear 1 and gear 2, and achieves adaptive adjustment of the fan speed, thereby controlling the mixing temperature; S4, condensation and circulation: the condensed liquid in the condensation box is connected to the channel in the stirring rod through the liquid inlet pipe and the liquid outlet pipe, and the circulation of the condensed liquid is realized under the action of the conveying component. The condensed liquid flows in the stirring rod, taking away the heat generated during the mixing process, ensuring the stability of the reaction temperature; S5. Discharging and cleaning: After the mixing reaction is completed, the mixture is discharged from the discharge pipe, and then the stirring rod and the inner wall of the shell are cleaned to ensure the cleanliness of the equipment and the sterility of the next use.

[0024] In summary, the present application includes at least one of the following beneficial technical effects: 1. The fan speed can be precisely adjusted by the design of the speed regulating mechanism. The diameters of gear 1 and gear 2 in the speed regulating mechanism change in opposite directions, and the fan speed can be automatically adjusted according to the temperature change in the housing. The bimetallic strip and the first drive assembly are arranged to make the temperature adjustment more sensitive and precise. This precise temperature adjustment mechanism can ensure that the temperature in the housing is always in a state suitable for antibody production, thereby improving the mixing efficiency of materials and the quality of antibody products. At the same time, by precisely controlling the fan speed, unnecessary energy waste is avoided, and energy-saving effects are achieved. 2. The design of the pressure-sensing diaphragm and the driving mechanism realizes the automatic adjustment of the pressure inside the shell; the pressure-sensing diaphragm can monitor the pressure changes inside the shell in real time, and automatically adjust the opening and closing of the ball valves in the air inlet and outlet pipes through the driving mechanism to ensure the pressure stability during the mixing process; this automatic pressure adjustment mechanism can ensure that the pressure inside the shell is always in a state suitable for antibody production, improving the automation and reliability of the equipment; by automatically adjusting the opening and closing of the ball valve, the problem of uneven mixing or insufficient reaction caused by pressure fluctuations is avoided, thereby improving the quality and production efficiency of antibody products; 3. The grinding chamber and screening device in the feed pipe can realize pre-crushing and screening of materials; the setting of the grinding plate and the screen plate can effectively grind and screen the materials to ensure uniform particle size of the materials; the vertical and horizontal movement of the grinding plate, as well as the horizontal movement of the screen plate, further improve the grinding and screening effects; this efficient material pretreatment mechanism can significantly increase the specific surface area of ​​the material, so that the material can be more fully in contact with the reaction medium or enzyme, thereby improving the reaction rate and efficiency; at the same time, by ensuring the uniform particle size of the materials, the influence of large particles on the mixing effect is avoided, the uniformity of mixing and the stability of the reaction are improved, and the quality and production efficiency of the antibody products are further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the overall structure of an antibody preparation device mainly embodied in an embodiment of the present application; Figure 2 This is a schematic diagram of the top block structure mainly embodied in the embodiment of the present application; Figure 3 for Figure 2 The enlarged view of point A in the middle; Figure 4 This is a schematic diagram of the condensation box structure mainly embodied in the embodiment of the present application; Figure 5 for Figure 4 The enlarged view of point B in the middle; Figure 6 This is a schematic diagram of the partition structure mainly embodied in the embodiment of the present application; Figure 7 This is a schematic diagram of a T-shaped guide block structure mainly embodied in an embodiment of the present application; Figure 8 This is a schematic diagram of a cam structure mainly embodied in an embodiment of the present application.

[0026] Figure numerals: 1, housing; 2, air inlet pipe; 3, air outlet pipe; 4, feed pipe; 40, stirring rod; 5, support plate; 6, support rod one; 7, fan; 8, speed regulating mechanism; 81, rotating rod one; 82, rotating rod two; 83, rotating rod three; 84, bevel gear one; 85, bevel gear two; 86, support rod two; 87, bevel gear three; 88, bevel gear four; 89, support rod three; 891, bevel gear five; 892, bevel gear six; 893, gear one ;894, gear 2;895, positioning block;896, positioning groove;90, first drive assembly;901, bimetallic strip;902, drive rod 1;903, drive rod 2;904, drive rod 3;905, connecting block 1;906, top block;907, reset spring 1;908, reset block 1;909, reset spring 2;910, reset block 2;9, pressure sensing diaphragm;10, ball valve;11, drive mechanism;111 , rack; 112, gear three; 113, drive rod four; 114, connecting block two; 115, reset spring three; 12, condenser; 13, connecting pipe one; 14, connecting pipe two; 15, liquid inlet pipe; 16, liquid outlet pipe; 17, conveying assembly; 171, piston rod; 172, rotating plate; 173, connecting rod one; 18, partition; 19, grinding chamber; 20, conveying chamber; 21, sieve plate; 22, slide plate; 23, telescopic rod; 24, grinding Grinding plate; 25. Second driving assembly; 251. Driving rod five; 252. Driving plate; 253. Belt; 254. Connecting rod two; 26. Third driving assembly; 261. Connecting plate; 262. Push block one; 263. Push block two; 264. T-shaped guide block; 265. Guide groove; 266. Push spring one; 27. Fourth driving assembly; 271. Driving rod six; 272. Cam; 273. Push spring two; 274. Driving block. DETAILED DESCRIPTION

[0027] The following is combined with Figure 1-8 This application is described in further detail.

[0028] The embodiments of the present application disclose an antibody preparation device and an antibody preparation process.

[0029] An antibody preparation device, referring to Figure 1, including a shell 1, an air inlet pipe 2, an air outlet pipe 3, a feed pipe 4 and a stirring rod 40. The outer wall of the shell 1 is fixedly connected to a support plate 5, and the top of the support plate 5 is fixedly connected to a support rod 6. A fan 7 is arranged outside the shell 1. The rotating shaft of the fan 7 is horizontally penetrated and rotatably connected to the support rod 6. A speed regulating mechanism 8 for adjusting the speed of the fan 7 is also arranged outside the shell 1; a through hole is opened on the outer wall of the shell 1, and a pressure sensing diaphragm 9 is fixedly connected in the through hole. Ball valves 10 are installed in the air inlet pipe 2 and on the air outlet pipe 3. A driving mechanism 11 for driving the valve body in the ball valve 10 to rotate is arranged outside the shell 1.

[0030] Reference Figure 1 When the materials are mixed and stirred in the shell 1, the stirring rod 40 can rotate and drive the fan 7 to rotate through the speed regulating mechanism 8. The rotation of the fan 7 can cool the inner cavity of the shell 1. If the temperature in the shell 1 is gradually too high, the speed regulating mechanism 8 is triggered at this time. The speed regulating mechanism 8 can adjust the speed of the fan 7 to increase the speed of the fan 7, thereby accelerating the reduction of the temperature in the inner cavity of the shell 1; the pressure sensing diaphragm 9 provided can monitor the pressure change inside the shell 1 in real time. When the pressure in the shell 1 gradually increases, the pressure sensing diaphragm 9 can be deformed and trigger the driving mechanism 11. The driving mechanism 11 can adjust the opening and closing of the ball valve 10, so that the ball valve 10 in the air inlet pipe 2 is gradually closed, and the ball valve 10 in the air outlet pipe 3 is gradually opened, ensuring the pressure stability during the mixing process; the whole process ensures that the temperature and pressure of the inner cavity of the shell 1 are in a state suitable for antibody production, thereby improving the mixing efficiency of the materials and the quality of the antibody products.

[0031] Reference Figure 1 The speed regulating mechanism 8 includes a rotating rod 1 81, a rotating rod 2 82 and a rotating rod 3 83. The rotating rod 1 81 is vertically arranged outside the housing 1, and the rotating rod 1 81 is penetrated and rotatably connected to the support plate 5. A bevel gear 1 84 is sleeved and fixed on the rotating shaft of the fan 7. A bevel gear 2 85 is sleeved and fixed on the top of the rotating rod 1 81. The bevel gear 2 85 is meshed with the bevel gear 1 84. A support rod 2 86 is fixedly connected to the bottom of the support plate 5. The rotating rod 2 82 is horizontally penetrated and rotatably connected to the support rod 2 86. The rotating rod 1 81 A bevel gear three 87 is sleeved and fixed on the upper part, a bevel gear four 88 is sleeved and fixed on one end of the rotating rod two 82, and the bevel gear four 88 is meshed with the bevel gear three 87; a support rod three 89 is fixedly connected to the bottom of the shell 1, and the rotating rod three 83 is horizontally penetrated and rotatably connected to the support rod three 89, and a bevel gear five 891 is sleeved and fixed on one end of the rotating rod three 83, and the bottom end of the stirring rod 40 extends to the outside of the shell 1 and is sleeved and fixed with a bevel gear six 892, and the bevel gear six 892 is meshed with the bevel gear five 891.

[0032] Reference Figure 1 , Figure 2 and Figure 3, the central axis of the rotating rod 2 82 is parallel to the central axis of the rotating rod 3 83, and a plurality of gears 893 of different diameters are sleeved and rotatably connected on the rotating rod 2 82, and the plurality of gears 893 are arranged in sequence along the central axis of the rotating rod 2 82 according to the diameter size; a plurality of gears 2 894 of different diameters are sleeved and fixed on the rotating rod 3 83, and the plurality of gears 2 894 are arranged in sequence along the central axis of the rotating rod 3 83 according to the diameter size, and the change trend of the diameter size of the plurality of gears 1 893 and the plurality of gears 2 894 along the same direction is opposite, and the plurality of gears 1 893 and the plurality of gears 2 894 are meshed with each other in a one-to-one correspondence; a plurality of positioning blocks 895 are slidingly arranged on the inner wall of the rotating rod 2 82 corresponding to each gear 1 893, and a positioning groove 896 for the positioning block 895 to be inserted is opened on the inner wall of the gear 1 893, and the speed regulating mechanism 8 also includes a first driving component 90 for driving the positioning block 895 to move.

[0033] Reference Figure 1 , Figure 2 and Figure 3 At the initial stage of mixing and stirring the materials, the positioning block 895 corresponding to the gear 1 893 with a larger diameter is inserted into the positioning groove 896 in the gear 1 893 with a larger diameter under the drive of the first driving assembly 90. At this time, the gear 1 893 with a larger diameter is fixed to the rotating rod 2 82, the stirring rod 40 drives the bevel gear 6 892 to rotate, the bevel gear 6 892 drives the bevel gear 5 891 and the rotating rod 3 83 to rotate, and the rotating rod 3 83 drives multiple gear 2 894 to rotate. When the gear 1 893 with a larger diameter is fixed to the rotating rod 2 82, the corresponding gear 2 894 can drive it to rotate, the gear 1 893 with a larger diameter drives the rotating rod 2 82 to rotate, the rotating rod 2 82 drives the bevel gear 4 88 to rotate, the bevel gear 4 88 drives the bevel gear 3 87 and the rotating rod 1 81 to rotate, and the rotating rod 1 81 drives the bevel gear 2 85 to rotate , bevel gear 2 85 drives bevel gear 1 84 and the rotating shaft of fan 7 to rotate, and the rotating speed of fan 7 is relatively low at this time; when the temperature in shell 1 gradually becomes too high, the first driving assembly 90 is triggered at this time, and the first driving assembly 90 drives the positioning block 895 corresponding to the smaller diameter gear 1 893 to be inserted into the positioning groove 896 of the corresponding gear 1 893, and the smaller diameter gear 1 893 is fixed to the rotating rod 2 82, and the larger diameter gear 1 893 is separated from the corresponding positioning block 895, and the rotating speed of fan 7 is increased at this time. When the temperature in shell 1 continues to rise, the first driving assembly 90 can fix the smaller diameter gear 1 893 to the rotating rod 2 82, so that the rotating speed of fan 7 is further increased. The whole process facilitates accurate speed regulation of fan 7, thereby facilitating temperature regulation in shell 1.

[0034] Reference Figure 2 , Figure 3 and Figure 4The first driving assembly 90 includes a bimetallic strip 901, a driving rod 1 902, a driving rod 2 903 and a driving rod 3 904. The bimetallic strip 901 is installed on the inner wall of the shell 1. The inner wall of the shell 1 is fixedly connected with a connecting block 1 905. The driving rod 1 902 is located in the shell 1, and the driving rod 1 902 is vertically penetrated and slidably connected to the connecting block 1 905. The top end of the driving rod 1 902 abuts against the bimetallic strip 901, and the bottom end of the driving rod 1 902 is penetrated to the outside of the shell 1. The driving rod 3 904 slides in the rotating rod 2 82 along the central axis direction of the rotating rod 2 82. The two ends of the driving rod 2 903 are respectively hinged to the driving rod 1 902 and A driving rod 3 904 is provided with a plurality of top blocks 906 which are sleeved and rotatably connected thereto. The plurality of top blocks 906 are arranged in a one-to-one correspondence with the plurality of positioning blocks 895. Inclined surfaces are provided on opposite sides of the top blocks 906. The top blocks 906 are slidably matched with the top blocks 906 through the inclined surfaces on both sides thereof, and each top block 906 is matched with each positioning block 895 asynchronously. The rotating rod 2 82 is also provided with a reset member 1 for resetting the positioning block 895. The reset member 1 is a reset spring 1 907. A reset block 1 908 is fixedly connected to the positioning block 895. The two ends of the reset spring 1 907 are respectively fixedly connected to the reset block 1 908 and the inner wall of the rotating rod 2 82.

[0035] Reference Figure 4 and Figure 5 A reset member 2 is also provided in the housing 1 for resetting the driving rod 1 902. The reset member 2 is a reset spring 2 909. The reset spring 2 909 is vertically sleeved on the driving rod 1 902. A reset block 2 910 is fixedly connected to the driving rod 1 902. Both ends of the reset spring 2 909 are respectively fixedly connected to the reset block 2 910 and the connecting block 1 905.

[0036] Reference Figure 2 , Figure 3 , Figure 4 and Figure 5When the temperature in the housing 1 rises, the bimetallic strip 901 deforms when the temperature changes, thereby pushing the driving rod 1 902 to move downward, the return spring 2 909 is stretched, the driving rod 1 902 drives the driving rod 2 903 to rotate, the driving rod 2 903 drives the driving rod 3 904 to move, and the driving rod 3 904 drives multiple top blocks 906 to move at the same time. At this time, the top block 906 corresponding to the smaller diameter gear 1 893 can push the corresponding positioning block 895 through its inclined surface to be inserted into the positioning groove 896 on the corresponding gear 1 893, and the return spring 1 907 is stretched. The bimetallic strip 901 is stretched so that the gear 1 893 is fixed to the rotating rod 2 82, and the top block 906 corresponding to the larger diameter gear 1 893 is away from the corresponding positioning block 895. The positioning block 895 can be away from the corresponding gear 1 893 under the elastic force of the reset spring 1 907. Similarly, when the temperature in the shell 1 gradually increases, the bimetallic strip 901 can gradually fix the smaller diameter gear 1 893 to the rotating rod 2 82, so that the rotation speed of the fan 7 is gradually increased, and the reset spring 2 909 can reset the driving rod 1 902.

[0037] Reference Figure 1 The driving mechanism 11 includes a rack 111, a gear three 112 and a driving rod four 113. A connecting block two 114 is fixedly connected to the outer wall of the shell 1. The rack 111 is horizontally penetrated and slides on the connecting block two 114. One end of the rack 111 abuts against the outer wall of the pressure sensing diaphragm 9. The two ends of the driving rod four 113 are respectively fixedly connected to the rotating shaft of the valve body of the ball valve 10 in the intake pipe 2 and the rotating shaft of the valve body of the ball valve 10 in the outlet pipe 3. The gear three 112 is sleeved and fixed on the driving rod four 113, and the rack 111 is meshed with the gear three 112. A reset member three for resetting the rack 111 is provided outside the shell 1. The reset member three is a reset spring three 115. The reset spring three 115 is horizontally arranged, and the two ends of the reset spring three 115 are respectively fixedly connected to the connecting block two 114 and the outer wall of the shell 1.

[0038] Reference Figure 1 The driving mechanism 11 can automatically adjust the opening and closing of the ball valve 10 according to the pressure change inside the shell 1. When the pressure inside the shell 1 gradually increases, the pressure sensing diaphragm 9 is deformed and pushes the rack 111 to move, and then drives the driving rod 4 113 to rotate through the gear three 112, so that the opening and closing degree of the ball valve 10 in the air inlet pipe 2 is reduced, and the opening and closing degree of the ball valve 10 in the air outlet pipe 3 is increased, so that the equipment can automatically adjust the air intake and air outlet according to the internal pressure, ensure the pressure stability during the mixing process, and improve the automation and reliability of the equipment.

[0039] Reference Figure 1 and Figure 4A condensation box 12 for storing condensed liquid is arranged outside the shell 1, and a heat sink is installed on the outer wall of the condensation box 12. A channel for the circulation of condensed liquid is opened in the stirring rod 40. A connecting pipe 13 and a connecting pipe 2 14 are rotatably sealed on the stirring rod 40. The side walls of the condensation box 12 are respectively connected with a liquid inlet pipe 15 and a liquid outlet pipe 16; the connecting pipe 13 is respectively connected to the outlet of the channel in the stirring rod 40 and the liquid inlet pipe 15, and the connecting pipe 2 14 is respectively connected to the inlet of the channel in the stirring rod 40 and the liquid outlet pipe 16, and a one-way valve is arranged in the liquid inlet pipe 15 and the liquid outlet pipe 16; a conveying component 17 for conveying condensed liquid is arranged outside the shell 1.

[0040] Reference Figure 1 and Figure 4 The rotating rod 82 can trigger the conveying assembly 17 while rotating. The conveying assembly 17 can make the condensed liquid circulate through the channel in the stirring rod 40 to take away the heat and ensure the stability of the reaction temperature; the condensation box 12 can effectively take away the heat generated during the mixing process. The heat sink further improves the heat dissipation effect of the condensation box 12 to maintain the stability of the reaction temperature; when the condensed liquid flows in the stirring rod 40, it exchanges heat with the material outside the stirring rod 40, and can quickly absorb the heat of the material, thereby reducing the temperature of the material. This direct heat exchange method further improves the cooling efficiency of the equipment.

[0041] Reference Figure 1 and Figure 4 The conveying assembly 17 includes a piston rod 171, a rotating plate 172 and a connecting rod 173. One end of the piston rod 171 is horizontally slidably connected to the inner wall of the condensation box 12, the rotating plate 172 is fixedly connected to the bottom end of the rotating rod 82, one end of the connecting rod 173 is rotatably connected to the bottom side wall of the rotating plate 172, and the other end of the connecting rod 173 is hinged to the end of the piston rod 171.

[0042] Reference Figure 1 and Figure 4 When the rotating rod 82 rotates, it can drive the rotating plate 172 to rotate, and the rotating plate 172 drives the connecting rod 173 to rotate, and the connecting rod 173 drives the piston rod 171 to reciprocate, thereby realizing the transportation of the condensed liquid. At the same time, when the temperature of the equipment gradually increases, the rotation speed of the rotating rod 82 increases, so the movement rate of the piston rod 171 increases. As the temperature increases, the condensed liquid absorbs more heat in the stirring rod 40, and the movement of the piston rod 171 is accelerated, which accelerates the circulation of the condensed liquid and performs faster heat exchange with the material outside the stirring rod 40, thereby more effectively reducing the material temperature and further improving the efficiency of the heat exchange.

[0043] Reference Figure 1 and Figure 6A partition 18 is fixedly connected to the inner wall of the feed pipe 4, and the partition 18 is arranged horizontally. The partition 18 divides the inner cavity of the feed pipe 4 into a grinding cavity 19 and a conveying cavity 20 connected to the inner cavity of the shell 1, and the grinding cavity 19 is located above the conveying cavity 20; a through hole is opened on the partition 18, and a sieve plate 21 is installed in the through hole. A slide plate 22 is vertically slid in the grinding cavity 19, and a plurality of telescopic rods 23 are arranged above the slide plate 22. The telescopic rods 23 are vertically arranged, and the two ends of the telescopic rods 23 are respectively fixedly connected to the top of the slide plate 22 and the inner top of the feed pipe 4; a grinding plate 24 is arranged on the side of the slide plate 22 close to the sieve plate 21.

[0044] Reference Figure 1 and Figure 6 A second driving assembly 25 for driving the grinding plate 24 to move vertically is provided outside the shell 1. The second driving assembly 25 includes a driving rod 5 251, a driving plate 252, a connecting rod 254 and a belt 253. The driving rod 5 251 is vertically arranged, and the driving rod 5 251 is penetrated and rotatably connected to the side wall of the feed pipe 4. The driving plate 252 is in the shape of a circular plate, and the driving plate 252 is located in the feed pipe 4, and the driving plate 252 is fixedly connected to the bottom end of the driving rod 5 251. The two ends of the connecting rod 254 are respectively ball-connected to the bottom of the driving plate 252 and the top of the slide plate 22. The connection between the connecting rod 254 and the driving plate 252 is located at the eccentric position of the driving plate 252; rollers are mounted and fixed on the stirring rod 40 and the driving rod 5 251, and the belt 253 is driven through the rollers on the stirring rod 40 and the driving rod 5 251.

[0045] Reference Figure 1 and Figure 6 The material first enters the grinding chamber 19, and the stirring rod 40 can drive the belt 253 while rotating. The belt 253 drives the driving rod 5 251 to rotate, and the driving rod 5 251 drives the driving plate 252 to rotate. The driving plate 252 drives the slide plate 22 and the grinding plate 24 to move repeatedly in the vertical direction through the connecting rod. The telescopic rod 23 can guide the slide plate 22, and the grinding plate 24 can repeatedly grind and roll the material; thereby, the material is pre-crushed and screened to ensure that the material particle size is uniform. The setting of the screen plate 21 can screen the material to prevent large particles from entering the mixing chamber. Grinding and crushing can significantly increase the specific surface area of ​​the material, so that the material can be more fully in contact with the reaction medium or enzyme, thereby improving the reaction rate and efficiency; grinding and crushing can make materials of different components more evenly mixed, which helps to improve the uniformity and consistency of the reaction.

[0046] Reference Figure 1 , Figure 6 and Figure 7The grinding plate 24 slides horizontally on the side of the slide plate 22 close to the screen plate 21. A third driving assembly 26 for driving the grinding plate 24 to move horizontally is provided in the feed pipe 4. The third driving assembly 26 includes a connecting plate 261, which is fixedly connected to the inner wall of the feed pipe 4. A plurality of push blocks 262 are fixedly connected in sequence along the vertical direction on the side of the connecting plate 261 close to the grinding plate 24. A push block 263 is fixedly connected to the side of the grinding plate 24 close to the connecting plate 261. A curved surface is provided on the side of the push block 263 close to the push block 1 262. The push block 263 is slidingly connected to the push block 1 262 through its curved surface; a T-shaped guide block 264 is fixed on the top of the grinding plate 24, and a guide groove 265 for the T-shaped guide block 264 to slide is provided at the bottom of the slide plate 22 in the horizontal direction. A push spring 266 is horizontally arranged in the guide groove 265, and the two ends of the push spring 266 are respectively fixedly connected to the T-shaped guide block 264 and the inner wall of the slide plate 22.

[0047] Reference Figure 1 , Figure 6 and Figure 7 During the vertical movement of the grinding plate 24, the grinding plate 24 drives the push block 263 to move vertically. The push block 263 can be continuously slidably connected with multiple push blocks 262 through its arc surface. The grinding plate 24 can move repeatedly in the horizontal direction under the action of the push block 262 and the push spring 1 266. The horizontal movement of the grinding plate 24 can further grind the material, ensure the uniform particle size of the material, improve the grinding effect, and ensure the uniformity of the material during the mixing process.

[0048] Reference Figure 1 , Figure 6 and Figure 8 The sieve plate 21 slides horizontally in the side wall of the partition 18, and a fourth driving assembly 27 for driving the sieve plate 21 to move horizontally is provided outside the shell 1. The fourth driving assembly 27 includes a driving rod 6 271, a cam 272 and a pushing spring 273. The driving rod 6 271 is vertically arranged, and the driving rod 6 271 is penetrated and rotatably connected to the feed pipe 4 and the side wall of the partition 18. The cam 272 is located in the feed pipe 4 and is located below the partition 18. The cam 272 is fixedly connected to the bottom end of the driving rod 6 271. A driving block 274 is fixedly connected to the bottom of the sieve plate 21, and the driving block 274 is slidingly connected to the side wall of the cam 272; the pushing spring 273 is horizontally arranged, and the two ends of the pushing spring 273 are respectively fixedly connected to the sieve plate 21 and the inner wall of the partition 18; a roller is fixedly mounted on the top of the driving rod 6 271, and the belt 253 is simultaneously mounted on the roller on the driving rod 6.

[0049] Reference Figure 1 , Figure 6 and Figure 8The horizontal movement of the sieve plate 21 can screen the materials and prevent large particles from entering the mixing chamber. When the grinding plate 24 is grinding, the stirring rod 40 can drive the driving rod 6 271 to rotate at the same time through the belt 253. The driving rod 6 271 drives the cam 272 to rotate. The side wall of the cam 272 can push the driving block 274 and the sieve plate 21 to move in the horizontal direction. The sieve plate 21 can reciprocate in the horizontal direction under the action of the cam 272 and the push spring 273, thereby accelerating the speed at which the ground materials fall into the conveying chamber 20 and reducing the possibility of material blockage above the sieve plate 21.

[0050] The implementation principle of the embodiment of the present application is as follows: when the materials are mixed and stirred in the shell 1, the stirring rod 40 can rotate while driving the fan 7 to rotate through the speed regulating mechanism 8. The rotation of the fan 7 can cool the inner cavity of the shell 1. If the temperature in the shell 1 gradually becomes too high, the speed regulating mechanism 8 is triggered at this time. The speed regulating mechanism 8 can adjust the speed of the fan 7 to increase the speed of the fan 7, thereby accelerating the reduction of the temperature in the inner cavity of the shell 1; the set pressure sensing diaphragm 9 can monitor the pressure change inside the shell 1 in real time. When the pressure in the shell 1 gradually increases, the pressure sensing diaphragm 9 can be deformed and trigger the driving mechanism 11. The driving mechanism 11 can adjust the opening and closing of the ball valve 10, so that the ball valve 10 in the air inlet pipe 2 is gradually closed, and the ball valve 10 in the air outlet pipe 3 is gradually opened, ensuring the pressure stability during the mixing process; the whole process ensures that the temperature and pressure of the inner cavity of the shell 1 are in a state suitable for antibody production, thereby improving the mixing efficiency of the materials and the quality of the antibody products.

[0051] A process for preparing an antibody comprises the following steps: S1. Material preparation and feeding: Antigens, adjuvants and other materials are added into the housing 1 through the feed pipe 4. The grinding chamber 19 in the feed pipe 4 pre-crushes and screens the materials through the grinding plate 24 and the sieve plate 21. The grinding plate 24 moves vertically through the second drive assembly 25, and the sieve plate 21 moves horizontally through the fourth drive assembly 27 to ensure uniform particle size of the materials and avoid large particles affecting the mixing effect; S2, mixing and stirring: starting the stirring rod 40, the stirring rod 40 can stir and mix the materials in the shell 1, under the stirring of the stirring rod 40, the materials are fully mixed in the shell 1 to form a uniform mixture; S3, temperature and pressure regulation: the pressure sensing diaphragm 9 outside the housing 1 monitors the internal pressure in real time, and adjusts the ball valve 10 in the air inlet pipe 2 and the air outlet pipe 3 through the driving mechanism 11 to achieve precise pressure control; the bimetallic strip 901 controls the movement of the positioning block 895 through the driving rod 1 902, the driving rod 2 903 and the driving rod 3 904, adjusts the meshing of the gear 1 893 and the gear 2 894, and realizes the adaptive adjustment of the speed of the fan 7, thereby controlling the mixing temperature; S4, condensation and circulation: the condensed liquid in the condensation tank 12 is connected to the channel in the stirring rod 40 through the liquid inlet pipe 15 and the liquid outlet pipe 16, and the circulation of the condensed liquid is realized under the action of the conveying component 17. The condensed liquid flows in the stirring rod 40, taking away the heat generated during the mixing process, and ensuring the stability of the reaction temperature; S5, discharging and cleaning: After the mixing reaction is completed, the mixture is discharged from the discharging pipe, and then the stirring rod 40 and the inner wall of the shell 1 are cleaned to ensure the cleanliness of the equipment and the sterility of the next use.

[0052] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. An antibody preparation device, comprising a housing (1), an air inlet pipe (2), an air outlet pipe (3), a feed pipe (4) and a stirring rod (40), characterized in that: A fan (7) is rotatably arranged outside the shell (1), and a speed regulating mechanism (8) for regulating the rotation speed of the fan (7) is also arranged outside the shell (1); a pressure sensing diaphragm (9) is arranged on the outer wall of the shell (1), a ball valve (10) is installed in the air inlet pipe (2) and on the air outlet pipe (3), and a driving mechanism (11) for driving the valve body in the ball valve (10) to rotate is arranged outside the shell (1).

2. The antibody preparation device according to claim 1, characterized in that: The speed regulating mechanism (8) comprises a rotating rod 1 (81), a rotating rod 2 (82) and a rotating rod 3 (83); the rotating rod 1 (81) is arranged outside the housing (1) for vertical rotation; a bevel gear 1 (84) is fixedly arranged on the rotating shaft of the fan (7); a bevel gear 2 (85) is fixedly arranged on the rotating rod 1 (81); the bevel gear 2 (85) is meshed with the bevel gear 1 (84); the rotating rod 2 (82) is arranged outside the housing (1) for horizontal rotation; the A bevel gear three (87) is fixedly provided on the rotating rod one (81), a bevel gear four (88) is fixedly provided on the rotating rod two (82), and the bevel gear four (88) is meshed with the bevel gear three (87); a bevel gear five (891) is fixedly provided on the rotating rod two (82), one end of the stirring rod (40) extends to the outside of the housing (1) and is fixedly provided with a bevel gear six (892), and the bevel gear six (892) is meshed with the bevel gear five (891); The central axis of the rotating rod 2 (82) is parallel to the central axis of the rotating rod 3 (83); a plurality of gears 1 (893) of different diameters are rotatably arranged on the rotating rod 2 (82); the plurality of gears 1 (893) are arranged in sequence along the central axis of the rotating rod 2 (82) according to their diameters; a plurality of gears 2 (894) of different diameters are fixedly arranged on the rotating rod 3 (83); the plurality of gears 2 (894) are arranged in sequence along the central axis of the rotating rod 3 (83) according to their diameters; the plurality of gears 1 (893) and the plurality of gears 2 (894) are fixedly arranged on the rotating rod 3 (83); the plurality of gears 1 (893) and the plurality of gears 2 (894) are arranged in sequence along the central axis of the rotating rod 3 (83) according to their diameters. The diameters of the gears 2 (894) change in opposite directions in the same direction, and the plurality of gears 1 (893) mesh with the plurality of gears 2 (894) in a one-to-one correspondence; the inner wall of the rotating rod 2 (82) is provided with a plurality of positioning blocks (895) corresponding to each gear 1 (893) for sliding, and the inner wall of the gear 1 (893) is provided with a positioning groove (896) for the positioning block (895) to be inserted; the speed regulating mechanism (8) further comprises a first driving component (90) for driving the positioning block (895) to move.

3. The antibody preparation device according to claim 2, characterized in that: The first driving assembly (90) comprises a bimetallic strip (901), a driving rod 1 (902), a driving rod 2 (903) and a driving rod 3 (904); the bimetallic strip (901) is installed in the housing (1); the driving rod 1 (902) slides in the housing (1); one end of the driving rod 1 (902) abuts against the bimetallic strip (901); the other end of the driving rod 1 (902) is passed through the outside of the housing (1); the driving rod 3 (904) slides in the rotating rod 2 (82); the two ends of the driving rod 2 (903) are respectively hinged to the driving rod 1 (902) and the driving rod 3 (904). The driving rod three (904) is rotatably provided with a plurality of top blocks (906), the plurality of top blocks (906) being arranged in one-to-one correspondence with the plurality of positioning blocks (895), the top blocks (906) being provided with inclined surfaces on opposite sides, the top blocks (906) being slidably matched with the top blocks (906) through the inclined surfaces on both sides thereof, and each top block (906) is matched with each positioning block (895) asynchronously; the rotating rod two (82) is also provided with a reset member one for resetting the positioning block (895), and the housing (1) is also provided with a reset member two for resetting the driving rod one (902).

4. The antibody preparation device according to claim 1, characterized in that: The driving mechanism (11) comprises a rack (111), a gear three (112) and a driving rod four (113); the rack (111) slides outside the housing (1); one end of the rack (111) abuts against the outer wall of the pressure sensing diaphragm (9); the two ends of the driving rod four (113) are respectively fixedly arranged on the rotating shaft of the valve body of the ball valve (10) in the inlet pipe (2) and the rotating shaft of the valve body of the ball valve (10) in the outlet pipe (3); the gear three (112) is fixedly arranged on the driving rod four (113), and the rack (111) is meshed with the gear three (112); and a reset member three for resetting the rack (111) is provided outside the housing (1).

5. The antibody preparation device according to claim 2, characterized in that: A condensation box (12) for storing condensed liquid is arranged outside the shell (1), and a heat sink is installed on the outer wall of the condensation box (12). A channel for circulating the condensed liquid is opened inside the stirring rod (40), and a connecting pipe 1 (13) and a connecting pipe 2 (14) are rotatably arranged on the stirring rod (40). The side walls of the condensation box (12) are respectively connected with a liquid inlet pipe (15) and a liquid outlet pipe (16); the connecting pipe 1 (13) is respectively connected to the outlet of the channel inside the stirring rod (40) and the liquid inlet pipe (15), and the connecting pipe 2 (14) is respectively connected to the inlet of the channel inside the stirring rod (40) and the liquid outlet pipe (16), and both the liquid inlet pipe (15) and the liquid outlet pipe (16) are provided with a one-way valve; and a conveying component (17) for conveying the condensed liquid is arranged outside the shell (1).

6. The antibody preparation device according to claim 5, characterized in that: The conveying assembly (17) comprises a piston rod (171), a rotating plate (172) and a connecting rod 1 (173); one end of the piston rod (171) is slidably connected to the inner wall of the condensing box (12); the rotating plate (172) is fixedly arranged on the rotating rod 2 (82); one end of the connecting rod 1 (173) is rotatably arranged on the rotating plate (172); and the other end of the connecting rod 1 (173) is hinged to the piston rod (171).

7. The antibody preparation device according to claim 1, characterized in that: A partition (18) is fixedly provided on the inner wall of the feed pipe (4), and the partition (18) divides the inner cavity of the feed pipe (4) into a grinding cavity (19) and a conveying cavity (20) connected to the inner cavity of the shell (1). A through hole is opened on the partition (18), and a sieve plate (21) is installed in the through hole. A slide plate (22) is vertically slidable in the grinding cavity (19), and a grinding plate (24) is provided on the side of the slide plate (22) close to the sieve plate (21). A second driving component (25) for driving the grinding plate (24) to move vertically is provided outside the shell (1).

8. The antibody preparation device according to claim 7, characterized in that: The grinding plate (24) slides horizontally on a side of the slide plate (22) close to the screen plate (21), and a third driving component (26) for driving the grinding plate (24) to move horizontally is provided in the feed pipe (4).

9. The antibody preparation device according to claim 7, characterized in that: The sieve plate (21) slides horizontally on the partition plate (18), and a fourth driving component (27) for driving the sieve plate (21) to move horizontally is provided outside the housing (1).

10. An antibody preparation process, based on an antibody preparation device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Material preparation and feeding: antigens, adjuvants and other materials are added into the housing (1) through the feed pipe (4). The grinding chamber (19) in the feed pipe (4) pre-crushes and screens the materials through the grinding plate (24) and the sieve plate (21). The grinding plate (24) moves vertically through the second drive assembly (25), and the sieve plate (21) moves horizontally through the fourth drive assembly (27), so as to ensure that the particle size of the materials is uniform and prevent large particles from affecting the mixing effect. S2, mixing and stirring: starting the stirring rod (40), the stirring rod (40) can stir and mix the materials in the shell (1), and under the stirring of the stirring rod (40), the materials are fully mixed in the shell (1) to form a uniform mixture; S3, temperature and pressure regulation: the pressure sensing diaphragm (9) outside the housing (1) monitors the internal pressure in real time, and adjusts the ball valve (10) in the air inlet pipe (2) and the air outlet pipe (3) through the driving mechanism (11), thereby realizing precise pressure control; the bimetallic strip (901) controls the movement of the positioning block (895) through the driving rod 1 (902), the driving rod 2 (903) and the driving rod 3 (904), and adjusts the meshing of the gear 1 (893) and the gear 2 (894), thereby realizing adaptive adjustment of the speed of the fan (7), thereby controlling the mixing temperature; S4, condensation and circulation: the condensed liquid in the condensation box (12) is connected to the channel in the stirring rod (40) through the liquid inlet pipe (15) and the liquid outlet pipe (16), and the circulation of the condensed liquid is realized under the action of the conveying component (17). The condensed liquid flows in the stirring rod (40) to take away the heat generated during the mixing process, thereby ensuring the stability of the reaction temperature; S5, discharging and cleaning: After the mixing reaction is completed, the mixture is discharged from the discharge pipe, and then the stirring rod (40) and the inner wall of the shell (1) are cleaned to ensure the cleanliness of the equipment and the sterility of the next use.

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