One-step forming process of mycelium composite fiber biological material

Abstract

The invention discloses a one-step forming process of a mycelium composite fiber biological material, and belongs to the technical field of mycelium composite materials. The one-step forming process of the mycelium composite fiber biological material comprises the following steps: screening a culture material which contains a well-grown strain and has a corresponding particle size matched with the size of a product mold; mixing the culture material with a setting agent, and uniformly stirring to obtain a mixed material; the product mold is filled with the mixed material, an inner cavity of the product mold is fully paved with the mixed material, then compression molding is performed, the molded mixed material is subjected to demolding treatment, then the molded mixed material is placed in a proper environment for subsequent mycelium culture and drying, and the mycelium composite biological material is obtained. The mycelium composite fiber biological material is fixed by adopting the setting agent, mycelia grow sufficiently under the pressing of a non-associated mold, a thick mycelium layer is formed on the surface of a product, and the consistency and uniformity of the appearance, the consistency of the size and the consistency of the weight of the product are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of mycelium composite materials, and in particular to a one-time molding process of mycelium composite fiber biomaterials. Background Art

[0002] In the current field of biomaterials, mycelium composite fiber biomaterials face a series of challenges and problems in the process of growth and molding. These problems include long mold occupancy time, large waste of resources, significant differences in molding effects of mass-produced products, and poor consistency in appearance and size. Traditional process methods usually require the use of independent molds to accompany the material growth process for a long time. This practice not only significantly increases the cost and space occupied by the mold, but also leads to the problem of white pollution. In addition, due to the limitations of the molding process, the mycelium layer of the product is often not thick enough, which directly affects the quality and performance of the product. At the same time, this process is also difficult to ensure that the same product maintains consistency in appearance, size, weight, etc., which makes it difficult to meet the strict requirements of industrial production for high efficiency, quality and stability. . Summary of the invention

[0003] The purpose of the present invention is to provide a one-time forming process of mycelium composite fiber biomaterial, which is used to solve the problem in the prior art that the mycelium layer of mycelium composite fiber biomaterial is not thick enough during the growth and forming process, thus affecting the quality and performance of the product.

[0004] The purpose of the present invention can be achieved through the following technical solutions: A one-step molding process for a mycelium composite fiber biomaterial, the one-step molding process for a mycelium composite fiber biomaterial comprising the following steps: A1. Select the culture medium with good growth of bacteria and corresponding particle size matching the product mold size; A2, taking the culture material and the setting agent, mixing, stirring evenly, to obtain a mixed material; A3. Fill the mixed material into the product mold and cover the inner cavity of the product mold, then press and form the mixed material. De-mold the formed mixed material, and then place it in a suitable environment for subsequent mycelium culture and drying to obtain the mycelium composite biomaterial.

[0005] As a further embodiment of the present invention, the preparation method of the setting agent comprises the following steps: S1, mix castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide, stir at 50-60°C for 10-15h, cool to room temperature, separate the liquid to take the oil layer, wash, extract, and vacuum dry to obtain material A; S2. In a nitrogen atmosphere, take the material A and ethanol, mix them, add dopamine hydrochloride and N,N-dimethylformamide, stir for 10-15 minutes, add zinc perchlorate hexahydrate, adjust the pH to 8, reflux and stir at 65-75°C for 15-25 hours, cool to room temperature, separate the liquid and take the oil layer, wash, extract, and vacuum dry to obtain material B; S3, in a nitrogen atmosphere, taking the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, and distilled water, mixing, stirring at 65-75° C. for 2-3 h, adding selenocystamine, stirring in an ice-water bath for 30-40 min, to obtain material C; S4, taking the material C, starch, willow fiber, microbial polysaccharide, and vitamins, mixing and stirring evenly to obtain the setting agent.

[0006] As a further solution of the present invention, in step S1, the mass ratio of castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide is 120-160:12-15:20-30:0.4-0.6:100-110.

[0007] As a further scheme of the present invention, in step S2, the dosage ratio of material A, ethanol, dopamine hydrochloride, N,N-dimethylformamide, and zinc perchlorate hexahydrate is 5-6g:10-12mL:1.8-2.1g:10-12mL:0.4-0.5g.

[0008] As a further scheme of the present invention, in step S3, the dosage ratio of material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine is 18-22mmol:55-65mmol:0.1g:6-8g:50-80mL:24-28mmol.

[0009] As a further solution of the present invention, in step S4, the mass ratio of material C, starch, willow fiber, microbial polysaccharide and vitamin is 2.4-3.2:10:4-5:1.5:1.

[0010] As a further solution of the present invention, in step A3, the pressing molding refers to pressing at a pressure of 0.4-0.8 MPa for 4-6 seconds.

[0011] As a further solution of the present invention, in step A2, the mass ratio of the culture medium to the setting agent is 7.2-8.5:1.5-2.5.

[0012] Beneficial effects of the present invention: The one-time forming process of a mycelium composite fiber biomaterial disclosed in the present invention uses a setting agent with certain biocompatibility and viscosity to adhere and fix the mycelium composite fiber biomaterial, and uses a product mold for short-pressure shaping, so that the culture material mixed with the biological setting agent can be evenly distributed in the mold cavity, ensuring that during the one-time pressing process, all parts of the product are evenly stressed, thereby ensuring the accuracy and consistency of the product shape, and then under the pressing without an accompanying mold, the mycelium grows fully, and the product surface has a thicker mycelium layer, ensuring that the product appearance is uniform, the size is consistent, and the weight is consistent. The mycelium is not restricted by the mold during the growth process, and can grow and interweave more fully, so that the product surface is covered with a thicker mycelium layer, improving the product's appearance, strength, toughness, texture and other properties. The present invention can effectively solve the problems existing in the existing mycelium composite fiber biomaterial molding technology, improve production efficiency and product quality, reduce resource waste and environmental pollution, and reduce the white pollution problem and poor product consistency caused by the traditional method requiring multiple molds for accompanying growth, and has significant economic and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present invention will be further described below in conjunction with the accompanying drawings.

[0014] Figure 1 It is a schematic diagram of a mycelium product obtained in Example 1 of a one-time molding process of a mycelium composite fiber biomaterial proposed in the present invention. DETAILED DESCRIPTION

[0015] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0016] Example 1 A one-step molding process of a mycelium composite fiber biomaterial comprises the following steps: A1. Select the culture medium containing well-growing Pleurotus ostreatus strains (purchased from Minyuan Edible Fungi Plant in Bayi Township, Wusheng County) and with the corresponding particle size matching the size of the product mold; The culture medium contains the following nutrients: Cottonseed husk 200.00g / L (extract); potato 200.00g / L (extract); glucose 20.00g / L; KH2PO4 3.00g / L; MgSO4·7H2O 1.50g / L; agar 18.00g / L; pH value natural conditions; A2, taking the culture material and the setting agent, mixing, stirring evenly, to obtain a mixed material; A3, taking the mixed material and filling it into the product mold and covering the inner cavity of the product mold, and then pressing and molding, demolding the molded mixed material, and then placing it in a suitable environment for subsequent mycelium cultivation and drying, so as to obtain the mycelium composite biomaterial, such as Figure 1 As shown, the hyphae are dense and uniform, with good adhesion; In step A3, the press molding refers to pressing at a pressure of 0.8 MPa for 5 seconds.

[0017] In step A2, the mass ratio of the culture medium to the setting agent is 7.2:1.5; The preparation method of the setting agent comprises the following steps: S1, castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide were mixed, stirred at 50°C for 10 hours, cooled to room temperature, separated and the oil layer was taken, washed, extracted and vacuum dried to obtain material A; The mass ratio of castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide is 120:12:20:0.4:100; the mass fraction of hydrogen peroxide is 30%; The washing and extraction specifically include the following steps: washing the oil layer with 100 mL of 3% sodium carbonate solution, washing with deionized water until neutral, and then separating with a separatory funnel, extracting the oil phase with 50 mL of ethyl acetate, and then drying with anhydrous sodium sulfate, filtering, and removing ethyl acetate by rotary evaporation, thus completing the washing and extraction operations; S2. In a nitrogen atmosphere, take the material A and ethanol, mix them, add dopamine hydrochloride and N,N-dimethylformamide, stir for 10 minutes, add zinc perchlorate hexahydrate, adjust the pH to 8, reflux and stir at 65°C for 15 hours, cool to room temperature, separate the liquid and take the oil layer, wash, extract, and vacuum dry to obtain material B; The dosage ratio of material A, ethanol, dopamine hydrochloride, N,N-dimethylformamide, and zinc perchlorate hexahydrate is 5g:10mL:1.8g:10mL:0.4g; The washing and extraction specifically include the following steps: washing the oil layer with 100 mL of hydrochloric acid solution with a pH of 2, then washing with deionized water until neutral, then separating with a separatory funnel, extracting the oil phase with 50 mL of ethyl acetate, then drying with anhydrous sodium sulfate, filtering, and removing ethyl acetate by rotary evaporation, thus completing the washing and extraction operations; S3. In a nitrogen atmosphere, the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite and distilled water were mixed, stirred at 65° C. for 2 h, selenocystamine was added, and stirred in an ice-water bath for 30 min to obtain material C; the dosage ratio of the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine was 18 mmol: 55 mmol: 0.1 g: 6 g: 50 mL: 24 mmol; S4, mixing the material C, starch, willow fiber, microbial polysaccharide and vitamins, and stirring evenly to obtain the setting agent; the mass ratio of the material C, starch, willow fiber, microbial polysaccharide and vitamins is 2.4:10:4:1.5:1; The preparation method of the willow fiber comprises the following steps: peeling and drying the tender stems of the willow, chopping them, immersing them in a 1 mol / L sodium hydroxide solution, stirring them at 80° C. for 2 hours, filtering them by suction, washing the solid phase, and drying them to obtain the willow fiber; The starch is potato starch; The microbial polysaccharide includes mannan (purchased from Shanghai PuKe Biotechnology Co., Ltd.) and proteoglycan (purchased from Shanghai TongWei Biotechnology Co., Ltd.), and the mass ratio of mannan to proteoglycan is 2:1; The vitamins include vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12, and the mass ratio of vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12 is 1:1:0.5:0.3:0.2:0.1.

[0018] Example 2 The molding process comprises the following steps: A1. Select the culture medium with the corresponding particle size that contains the well-growing oyster mushroom strains and matches the size of the product mold; A2, taking the culture material and the setting agent, mixing, stirring evenly, to obtain a mixed material; A3, filling the mixed material into the product mold and covering the inner cavity of the product mold, and then pressing and molding, demolding the molded mixed material, and then placing it in a suitable environment for subsequent mycelium cultivation and drying, so as to obtain the mycelium composite biomaterial, which has dense and uniform mycelium and good adhesion; In step A3, the press molding refers to pressing at a pressure of 0.4 MPa for 6 seconds.

[0019] In step A2, the mass ratio of the culture medium to the setting agent is 7.9:2.0; The preparation method of the setting agent comprises the following steps: S1. Mix castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide, stir at 55°C for 13h, cool to room temperature, separate the oil layer, wash, extract and vacuum dry to obtain material A; The mass ratio of castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide is 140:14:25:0.5:105; the mass fraction of hydrogen peroxide is 30%; S2. In a nitrogen atmosphere, take the material A and ethanol, mix them, add dopamine hydrochloride and N,N-dimethylformamide, stir for 12 minutes, add zinc perchlorate hexahydrate, adjust the pH to 8, reflux and stir at 70°C for 20 hours, cool to room temperature, separate the liquid and take the oil layer, wash, extract, and vacuum dry to obtain material B; The dosage ratio of material A, ethanol, dopamine hydrochloride, N,N-dimethylformamide, and zinc perchlorate hexahydrate is 5.5 g: 11 mL: 1.9 g: 11 mL: 0.45 g; S3. In a nitrogen atmosphere, the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite and distilled water were mixed, stirred at 70° C. for 2.5 h, selenocystamine was added, and stirred in an ice-water bath for 35 min to obtain material C; the dosage ratio of the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine was 20 mmol: 60 mmol: 0.1 g: 7 g: 65 mL: 26 mmol; S4, mixing the material C, starch, willow fiber, microbial polysaccharide and vitamins, stirring evenly to obtain the setting agent; the mass ratio of the material C, starch, willow fiber, microbial polysaccharide and vitamins is 2.4-3.2:10:4-5:1.5:1; The starch is potato starch; The microbial polysaccharide comprises mannan and proteoglycan, and the mass ratio of mannan to proteoglycan is 2:1; The vitamins include vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12, and the mass ratio of vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12 is 1:1:0.5:0.3:0.2:0.1.

[0020] Example 3 The molding process comprises the following steps: A1. Select the culture medium with the corresponding particle size that contains the well-growing oyster mushroom strains and matches the size of the product mold; A2, taking the culture material and the setting agent, mixing, stirring evenly, to obtain a mixed material; A3, filling the mixed material into the product mold and covering the inner cavity of the product mold, and then pressing and molding, demolding the molded mixed material, and then placing it in a suitable environment for subsequent mycelium cultivation and drying, so as to obtain the mycelium composite biomaterial, which has dense and uniform mycelium and good adhesion; In step A3, the press molding refers to pressing at a pressure of 0.6 MPa for 4 seconds.

[0021] In step A2, the mass ratio of the culture medium to the setting agent is 8.5:2.5; The preparation method of the setting agent comprises the following steps: S1. Mix castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide, stir at 60°C for 15h, cool to room temperature, separate the oil layer, wash, extract and vacuum dry to obtain material A; The mass ratio of castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide is 160:15:30:0.6:110; S2. In a nitrogen atmosphere, take the material A and ethanol, mix them, add dopamine hydrochloride and N,N-dimethylformamide, stir for 15 minutes, add zinc perchlorate hexahydrate, adjust the pH to 8, reflux and stir at 75°C for 25 hours, cool to room temperature, separate the liquid and take the oil layer, wash, extract, and vacuum dry to obtain material B; The dosage ratio of the material A, ethanol, dopamine hydrochloride, N,N-dimethylformamide, and zinc perchlorate hexahydrate is 6g:12mL:2.1g:12mL:0.5g; S3. In a nitrogen atmosphere, the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite and distilled water were mixed, stirred at 75° C. for 3 h, selenocystamine was added, and stirred in an ice-water bath for 40 min to obtain material C; the dosage ratio of the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine was 22 mmol: 65 mmol: 0.1 g: 8 g: 80 mL: 28 mmol; S4, mixing the material C, starch, willow fiber, microbial polysaccharide and vitamins, and stirring evenly to obtain the setting agent; the mass ratio of the material C, starch, willow fiber, microbial polysaccharide and vitamins is 3.2:10:5:1.5:1; The starch is potato starch; The microbial polysaccharide comprises mannan and proteoglycan, and the mass ratio of mannan to proteoglycan is 2:1; The vitamins include vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12, and the mass ratio of vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12 is 1:1:0.5:0.3:0.2:0.1.

[0022] Comparative Example 1 The difference from Example 1 is that no Salix chinensis fiber is added in the step S4 of preparing the setting agent. The mycelium of the obtained mycelium composite biomaterial is sparse and uneven, and has poor adhesion.

[0023] Compared with Example 1, in Comparative Example 1, no support of the Willow of Chestnut Tree fiber with a porous structure was added, which made it difficult for the mycelium to attach and extend, resulting in sparse and uneven mycelium and reduced adhesion.

[0024] Comparative Example 2 The difference from Example 1 is that the microbial polysaccharide in the preparation step S4 of the setting agent only includes mannan. The mycelium of the obtained mycelium composite biomaterial is sparse and uneven, and has poor adhesion.

[0025] Comparative Example 3 The difference from Example 1 is that the microbial polysaccharide in the preparation step S4 of the setting agent only includes proteoglycan. The mycelium of the obtained mycelium composite biomaterial is dense and uniform, and has poor adhesion.

[0026] It can be seen from Comparative Examples 2-3 that the present application helps to stabilize the structure of the mycelium mass, ensure the uniformity of mycelium growth, and provide a certain nutritional environment by introducing the synergistic effects of microbial polysaccharides mannan and proteoglycan.

[0027] Comparative Example 4 The difference from Example 1 is that nano-hydroxyapatite is not added in the preparation step S3 of the setting agent. The obtained mycelium composite biomaterial has dense and uneven mycelium and good adhesion. The addition of nano-hydroxyapatite can inhibit the growth of miscellaneous bacteria and improve the quality of mycelium.

[0028] Comparative Example 5 The difference from Example 1 is that the preparation method of the setting agent comprises the following steps: S1. In a nitrogen atmosphere, castor oil, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite and distilled water were mixed, stirred at 65° C. for 2 h, selenocystamine was added, and stirred in an ice-water bath for 30 min to obtain material C; the ratio of castor oil, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine was 18 mmol: 55 mmol: 0.1 g: 6 g: 50 mL: 24 mmol; S2, taking the material C, starch, willow fiber, microbial polysaccharide, and vitamins, mixing and stirring evenly to obtain the setting agent; the mass ratio of the material C, starch, willow fiber, microbial polysaccharide, and vitamins is 2.4:10:4:1.5:1; The preparation method of the willow fiber comprises the following steps: peeling and drying the tender stems of the willow, chopping them, immersing them in a 1 mol / L sodium hydroxide solution, stirring them at 80° C. for 2 hours, filtering them by suction, washing the solid phase, and drying them to obtain the willow fiber; The starch is potato starch; The microbial polysaccharide includes mannan (purchased from Shanghai PuKe Biotechnology Co., Ltd.) and proteoglycan (purchased from Shanghai TongWei Biotechnology Co., Ltd.), and the mass ratio of mannan to proteoglycan is 2:1; The vitamins include vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12, and the mass ratio of vitamin B1, vitamin B3, vitamin B5, vitamin B7, vitamin B9 and vitamin B12 is 1:1:0.5:0.3:0.2:0.1.

[0029] The obtained mycelium composite biomaterial has sparse mycelium and poor adhesion.

[0030] It can be seen from Comparative Example 5 that the present application, by modifying the castor oil chain segment and grafting dopamine, can promote the attachment of calcium elements and make the mycelium grow denser on the one hand, and improve the viscosity of the polyurethane glue and improve the product quality on the other hand.

[0031] The above describes some embodiments of the present invention in detail, but the contents are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims

1. A one-step molding process for mycelium composite fiber biomaterial, characterized in that: The one-step forming process of the mycelium composite fiber biomaterial comprises the following steps: A1. Select the culture medium with good growth of bacteria and corresponding particle size matching the product mold size; A2, taking the culture material and the setting agent, mixing, stirring evenly, to obtain a mixed material; A3. Fill the mixed material into the product mold and cover the inner cavity of the product mold, then press and form the mixed material. De-mold the formed mixed material, and then place it in a suitable environment for subsequent mycelium culture and drying to obtain the mycelium composite biomaterial.

2. The one-step forming process of a mycelium composite fiber biomaterial according to claim 1, characterized in that: The preparation method of the setting agent comprises the following steps: S1, mix castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide, stir at 50-60°C for 10-15h, cool to room temperature, separate the liquid to take the oil layer, wash, extract, and vacuum dry to obtain material A; S2. In a nitrogen atmosphere, take the material A and ethanol, mix them, add dopamine hydrochloride and N,N-dimethylformamide, stir for 10-15 minutes, add zinc perchlorate hexahydrate, adjust the pH to 8, reflux and stir at 65-75°C for 15-25 hours, cool to room temperature, separate the liquid and take the oil layer, wash, extract, and vacuum dry to obtain material B; S3, in a nitrogen atmosphere, taking the material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, and distilled water, mixing, stirring at 65-75° C. for 2-3 h, adding selenocystamine, stirring in an ice-water bath for 30-40 min, to obtain material C; S4, taking the material C, starch, willow fiber, microbial polysaccharide, and vitamins, mixing and stirring evenly to obtain the setting agent.

3. The one-step forming process of mycelium composite fiber biomaterial according to claim 2, characterized in that: In step S1, the mass ratio of castor oil, aluminum oxide, acetic acid, urea and hydrogen peroxide is 120-160:12-15:20-30:0.4-0.6:100-110.

4. The one-step forming process of a mycelium composite fiber biomaterial according to claim 2, characterized in that: In step S2, the dosage ratio of material A, ethanol, dopamine hydrochloride, N,N-dimethylformamide, and zinc perchlorate hexahydrate is 5-6 g: 10-12 mL: 1.8-2.1 g: 10-12 mL: 0.4-0.5 g.

5. The one-step forming process of mycelium composite fiber biomaterial according to claim 2, characterized in that: In step S3, the dosage ratio of material B, isophorone diisocyanate, dibutyltin dilaurate, nano-hydroxyapatite, distilled water and selenocystamine is 18-22mmol:55-65mmol:0.1g:6-8g:50-80mL:24-28mmol.

6. The one-step forming process of mycelium composite fiber biomaterial according to claim 2, characterized in that: In step S4, the mass ratio of material C, starch, willow fiber, microbial polysaccharide and vitamin is 2.4-3.2:10:4-5:1.5:

1.

7. The one-step forming process of mycelium composite fiber biomaterial according to claim 1, characterized in that: In step A3, the pressing molding refers to pressing at a pressure of 0.4-0.8 MPa for 4-6 seconds.

8. The one-step forming process of mycelium composite fiber biomaterial according to claim 1, characterized in that: In step A2, the mass ratio of the culture medium to the setting agent is 7.2-8.5:1.5-2.5.

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