Method for efficiently inhibiting invasion of cuscuta larakii by using submerged plants

Abstract

The invention discloses a method for efficiently inhibiting invasion of cuscuta larakii by using submerged plants. The method comprises the following steps: pre-culturing cuscuta larakii and submerged plants; disinfecting the body surface of the submerged plant; co-culturing the cuscuta larakii and the submerged plants; the growth and photosynthetic activity of the cusp lavieri are detected, the inhibition ability of the submerged plants to the cusp lavieri is evaluated, the submerged plants are selected from hornwort, hydrilla verticillata, myriophyllum spicatum, vallisneria spiralis and Setaria marina, especially vallisneria spiralis, the species take roots at the water bottom, long-term algal inhibition is continuously performed on a target water body, and the method is suitable for most of shallow water bodies and has a wide application prospect. And interspecific competition is fully carried out with the cuscuta larakii, so that higher cuscuta larakii inhibition capability is achieved. According to the method disclosed by the invention, a mode of co-culturing the submerged plant and the cuscuta larakii is used for efficiently inhibiting the cuscuta larakii, and theoretical guidance and technical support are provided for treatment of the cuscuta larakii.

Description

Technical Field

[0001] The invention relates to the technical field of water body restoration, and in particular to a method for efficiently inhibiting invasive Acutus lamarckii by utilizing submerged plants. Background Art

[0002] Submerged macrophytes refer to higher aquatic plants whose entire plant is located below the water surface and whose roots are fixed to the bottom mud or filler. Because submerged macrophytes are completely submerged below the water surface, all parts of the plant body can absorb water and nutrients, so they are very sensitive to environmental stress. Submerged macrophytes rely on their own roots, stems, and leaves to form a complete aerenchyma to exchange gases in oxygen-deficient water. In addition, submerged macrophytes can improve the transparency of water bodies, increase dissolved oxygen in water bodies, and change the pH value of water bodies through respiration, thereby affecting the physical and chemical indicators of water bodies. Since submerged macrophytes can inhibit algae through various pathways such as allelopathic effects and nutrient competition, they maintain a good water environment and promote the transformation of eutrophic water bodies to a clear water steady state. Therefore, submerged macrophytes play a vital role in the control of cyanobacterial blooms, and submerged macrophyte remediation technology is also one of the most promising water pollution remediation technologies.

[0003] With the eutrophication of water bodies and global warming, the frequency, intensity and range of cyanobacterial blooms have gradually increased worldwide, causing serious water ecological safety problems. In my country's freshwater waters, the most common cyanobacteria in blooms are Microcystis. In recent years, with global warming, new cyanobacteria with strong invasive and diffusion capabilities, such as Raphidiopsis raciborskii, have also been discovered and bloomed in my country. Raphidiopsis raciborskii is common in tropical deep water bodies and can tolerate adverse environmental conditions such as low light intensity and low nutrient levels. The pseudocylindrospermotoxin it produces is the second most common cyanobacterial toxin in the world. In deepwater reservoirs in subtropical areas of my country, the frequency of Raphidiopsis raciborskii blooms has gradually increased. In recent years, it has gradually replaced Microcystis and become the dominant population of cyanobacteria in reservoirs in southeastern my country. However, so far, research on its prevention and control is still very insufficient.

[0004] The use of physical and chemical methods to control Lassen's sharp algae is often costly and easily causes harm to other aquatic organisms. Among the biological methods that have been reported, Duchnik et al. co-cultured 25g / L duckweed with Lassen's sharp algae for 35 days, and the biomass of Lassen's sharp algae decreased by 25%, and the content of pseudocylindrosporin decreased by 32%. However, this biomass of duckweed is often difficult to achieve in natural water bodies, and the algae inhibition effect is not outstanding. In the prior art, the biological control method of Lassen's sharp algae is to co-cultivate duckweed with Lassen's sharp algae and observe the inhibition of Lassen's sharp algae, but there are the following problems: it is difficult to reach a sufficient number of duckweed under the water surface in open waters, the application scenarios are limited, and the inhibition efficiency of duckweed on Lassen's sharp algae is not high.

[0005] Therefore, it is necessary to screen out an aquatic organism that can effectively inhibit A. laevis and develop a safe and low-cost biological control method. Summary of the invention

[0006] In order to overcome the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a method for efficiently inhibiting the invasive C. laxmifolia by using submerged plants, and to screen a submerged plant, Vallisneria serrata, to inhibit C. laxmifolia. This species takes root in the water bottom and can continuously inhibit algae in the target water body for a long time, and is suitable for most shallow water bodies. At the same time, since the whole plant of this species is underwater, it can fully compete with C. laxmifolia among species, and has a higher ability to inhibit C. laxmifolia.

[0007] To achieve the above object, the present invention adopts the following technical solution:

[0008] A method for efficiently inhibiting the invasion of Acutus lamarckii by using submerged plants comprises the following steps:

[0009] Step 1, pre-cultivation of Acanthocephalum rabatii and submerged plants;

[0010] Step 2: Disinfection of submerged plant surface;

[0011] Step 3, co-cultivating Acanthocephalum rabatii and submerged plants;

[0012] Step 4, detecting the growth and photosynthetic activity of Acrocephalus lagerstroemia, and evaluating the inhibitory ability of the submerged plant on Acrocephalus lagerstroemia;

[0013] The submerged plants are selected from one or more of duckweed, black algae, foxtail algae, Vallisneria, and giant ferns.

[0014] Preferably, the Acanthocephalum lamarckii is purchased from the algae species bank of the Institute of Hydrobiology, Chinese Academy of Sciences, and cultured using BG11 culture medium to the logarithmic growth phase.

[0015] Preferably, the submerged plant is Vallisneria.

[0016] Preferably, the method for efficiently inhibiting the invasion of Acanthopanax rabatii by using submerged plants comprises:

[0017] Step 1: The acanthocephalum ragwort was cultured in BG11 medium until the logarithmic growth phase. The submerged plants were collected from natural water bodies in the wild, and the dead branches and leaves were removed. After being washed with tap water to remove surface attachments, they were cultured in 10% Hougland medium and set aside.

[0018] Step 2: Before use, soak the submerged plants in 10% sodium hypochlorite solution for 20 seconds to disinfect their surface, and then rinse with sterile water for more than 3 times to remove the residual sodium hypochlorite solution on the surface;

[0019] Step 3: Add the mother solution of Acrocephalus lagerstroemia in the logarithmic growth phase to the sterilized 10% BG11 medium to make its biological fresh weight reach 25 mg / L; select the top part with vigorous growth or retain the root of the submerged plant, cut plants of different weights and place them in the Acrocephalus lagerstroemia liquid for a total of 8 days;

[0020] Step 4: During the co-cultivation period, the biomass, chlorophyll a content, maximum photosynthetic efficiency and actual photosynthetic efficiency of the Aculeatus lagerstroemia are measured every day to evaluate the inhibitory ability of the submerged plants on the Aculeatus lagerstroemia.

[0021] More preferably, in step 3, the submerged plant is Vallisneria, and the roots are retained.

[0022] More preferably, in step 4, the biomass is calculated by measuring the absorbance at 750 nm using a spectrophotometer, and the chlorophyll a content, maximum photosynthetic efficiency and actual photosynthetic efficiency are measured using a phyto-PAM instrument.

[0023] Compared with the prior art, the beneficial effect of the present invention is that by co-cultivating submerged plants with Acuta lari, submerged plant species with high efficiency in inhibiting Acuta lari are screened, providing theoretical guidance and technical support for the control of Acuta lari, filling the gap in my country's research on the biological control of new water bloom cyanobacteria, and also providing a theoretical basis for steady-state conversion of aquatic ecosystems. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 : This is the change of biomass of Acanthopanax lagerstroemia in the co-cultivation conditions in the example.

[0025] Figure 2 This is the change of chlorophyll a of Acanthopanax lavarii under the co-cultivation conditions in the example.

[0026] Figure 3 : This is the change of the maximum photosynthetic efficiency (Fv / Fm) of Acrocephalum lagerstroemia in the co-cultivation conditions in the example.

[0027] Figure 4 The figure shows the change of the actual photosynthetic efficiency (Yield) of Acrocephalum lamarckii under the co-cultivation conditions in the embodiment.

[0028] Figure 5 The changes at the beginning and end of the experiment under the co-culture conditions in the example are shown. DETAILED DESCRIPTION

[0029] In order to more fully understand and demonstrate the technical solutions, purposes and advantages of the present invention, the technical effects produced by the present invention are further described in detail and completely in conjunction with the accompanying drawings and specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all. It should be pointed out that for those of ordinary skill in the art, other embodiments obtained without departing from the concept of the present invention all belong to the protection scope of the present invention.

[0030] The following example proposes a method for efficiently inhibiting the invasion of Acutus lamarckii by using submerged plants, comprising the following steps:

[0031] Step 1, pre-cultivation of Acanthocephalum rabatii and submerged plants;

[0032] Step 2: Disinfection of submerged plant surface;

[0033] Step 3, co-cultivating Acanthocephalum rabatii and submerged plants;

[0034] Step 4: Detect the growth and photosynthetic activity of Acrocephalus lagerstroemia, and evaluate the inhibitory ability of the submerged plants on Acrocephalus lagerstroemia.

[0035] Example 1

[0036] This embodiment provides a method for efficiently inhibiting the invasive cyanobacteria Acanthopanax raschii by using submerged plants, and the steps are as follows:

[0037] Step 1: Acanthopanax lamarckii was purchased from the algae seed bank of the Institute of Hydrobiology, Chinese Academy of Sciences, and cultured in BG11 medium until the logarithmic growth period; submerged plants - Dermatophyte, Hydrilla verticillata, Myriophyllum spicate, Vallisneria, and Megaspermum were selected and collected from natural water bodies in the wild, with dead branches and leaves removed, washed with tap water to remove surface attachments, and then cultured in 10% Hougland medium for later use.

[0038] Step 2: Before use, soak the submerged plants in 10% sodium hypochlorite solution for 20 seconds to disinfect their surface, and then rinse with sterile water for more than 3 times to remove the residual sodium hypochlorite solution on the surface.

[0039] Step 3: Add the mother solution of Acrocephala lagerstroemia in the logarithmic growth phase to the sterilized 10% BG11 medium to make its biological fresh weight reach 25 mg / L. For submerged plants, the top part with vigorous growth was selected, and for Vallisneria vallisneria, the roots were retained due to its morphological characteristics. Plants of different weights were cut and placed in the Acrocephala lagerstroemia liquid and cultured in the incubator for 8 days.

[0040] Step 4: During the co-cultivation period, the biomass, chlorophyll a content, maximum photosynthetic efficiency, and actual photosynthetic efficiency of Acrocephalum lagerstroemia were measured every day. The biomass was calculated by measuring the absorbance at 750 nm using a spectrophotometer, and the chlorophyll a content, maximum photosynthetic efficiency, and actual photosynthetic efficiency were measured using a phyto-PAM instrument.

[0041] In this example, the test results after 8 days of co-culturing of Acanthopanax lagerstroemia and submerged plants are as follows: Figure 1-5 As shown, it can be seen that Dermatophyta and Ceratium have lower tolerance to Acrocephalus laxmi, and the plants show obvious decay. Vallisneria has a better tolerance to Acrocephalus laxmi, and the plants are intact at the end, and the culture medium becomes clear and transparent, indicating that Acrocephalus laxmi is significantly inhibited. The results show that 0.5g / L Vallisneria can significantly inhibit the growth and photosynthetic activity of Acrocephalus laxmi, and the algae inhibition effect can reach more than 90%.

[0042] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for efficiently inhibiting the invasion of Acanthopanax rabatii by using submerged plants, characterized in that: The following steps are involved: Step 1, pre-cultivation of Acanthocephalum rabatii and submerged plants; Step 2: Disinfection of submerged plant surface; Step 3, co-cultivating Acanthocephalum rabatii and submerged plants; Step 4, detecting the growth and photosynthetic activity of Acrocephalus lagerstroemia, and evaluating the inhibitory ability of the submerged plant on Acrocephalus lagerstroemia; The submerged plants are selected from one or more of duckweed, black algae, foxtail algae, Vallisneria, and giant hydrilla.

2. The method for efficiently inhibiting the invasion of Acrocephalus lagerstroemia using submerged plants according to claim 1, characterized in that: The Acuta laevis was purchased from the algae seed bank of the Institute of Hydrobiology, Chinese Academy of Sciences, and cultured in BG11 medium until the logarithmic growth phase.

3. The method for efficiently inhibiting the invasion of Acrocephalus lagerstroemia by using submerged plants according to claim 1, characterized in that: The submerged plant is Vallisneria.

4. The method for efficiently inhibiting the invasion of Acrocephalus lagerstroemia by using submerged plants according to claim 1, characterized in that: The method comprises: Step 1: The Lacrocephalus was cultured in BG11 medium until the logarithmic growth phase. The submerged plants were collected from natural water bodies in the wild, and the dead branches and leaves were removed. After being washed with tap water to remove surface attachments, they were cultured in 10% Hougland medium and set aside. Step 2: Before use, soak the submerged plants in 10% sodium hypochlorite solution for 20 seconds to disinfect their surface, and then rinse with sterile water for more than 3 times to remove the residual sodium hypochlorite solution on the surface; Step 3: Add the mother solution of Acrocephalus lagerstroemia in the logarithmic growth phase to the sterilized 10% BG11 medium to make its biological fresh weight reach 25 mg / L. For submerged plants, select the top part with vigorous growth or retain the roots, cut plants of different weights and place them in the Acrocephalus lagerstroemia liquid for a total of 8 days; Step 4: During the co-cultivation period, the biomass, chlorophyll a content, maximum photosynthetic efficiency and actual photosynthetic efficiency of the Aculeatus lagerstroemia are measured every day to evaluate the inhibitory ability of the submerged plants on the Aculeatus lagerstroemia.

5. The method for efficiently inhibiting the invasion of Acrocephalus lagerstroemia using submerged plants according to claim 4, characterized in that: In step 3, the submerged plant is Vallisneria, and the roots are retained.

6. The method for efficiently inhibiting the invasion of Acrocephalus lagerstroemia by using submerged plants according to claim 4, characterized in that: In step 4, the biomass is calculated by measuring the absorbance at 750 nm using a spectrophotometer, and the chlorophyll a content, maximum photosynthetic efficiency and actual photosynthetic efficiency are measured using a phyto-PAM instrument.

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