Synergistic biological bactericide composition containing bacillus licheniformis and preparation method thereof
By reasonably proportioning Bacillus licheniformis and Bacillus cerebellum in pesticides, and combining appropriate additives to prepare biofungicide compositions into aqueous or suspending agents, the existing pesticides have been solved, with low efficiency, high toxicity, high residue and enhanced drug resistance, and the effective, low toxicity, and low residue prevention and control effects on economic crop diseases are achieved.
Patent Information
- Application Number
- CN202311338878.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-17
- Publication Date
- 2025-04-18
AI Technical Summary
Existing pesticides have problems such as low efficiency, high toxicity, high residue and enhanced drug resistance when preventing and controlling cash crop diseases, and lack highly efficient and broad-spectrum biopesticides.
A synergistic biofungicide composition containing Bacillus licheniformis and Bacillus cereci are used to prepare a water or suspension agent by reasonably comparing Bacillus licheniformis and Bacillus cereciniformis, and combining emulsifiers, dispersing wetting agents, antifreeze agents, thickening agents and defoaming agents.
It has achieved efficient prevention and control of cash crop diseases, delayed the occurrence of disease resistance, and has low toxicity, low residue, and broad-spectrum sterilization effects, reduced prevention and control costs, and is suitable for agricultural promotion and application.
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Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of pesticides, and in particular to a synergistic biofungicide composition containing Bacillus licheniformis and a preparation method thereof. Background Art
[0002] Cucumber downy mildew, watermelon wilt and tobacco brown spot are important diseases during the growth period of the above cash crops, with an annual incidence of over 100 million mu, which is a serious obstacle to the high and stable yield of cash crops in my country. The realization of pollution-free biological control of cash crop diseases is the development direction and goal of agricultural plant protection. Research and development of new pesticides with high efficiency, low toxicity and low residue is an inevitable development trend.
[0003] Bacillus licheniformis is a microbial fungicide. It is a bacterial disease prevention preparation fermented from Bacillus licheniformis using culture medium. It has a strong antagonistic effect on plant pathogenic fungi and can effectively prevent and control cucumber and tobacco diseases. However, as a biological pesticide, Bacillus licheniformis has a long lasting effect, but the product's quick-acting property is relatively poor.
[0004] Bacillus cereus can regulate the microhabitat of crop cells through the SOD enzyme in the body, maintain normal physiological metabolism and biochemical reactions of cells, improve stress resistance, accelerate growth, and increase yield and quality. Because the time limit for using only Bacillus cereus to prevent and control major diseases of major economic crops has been long, the resistance of crops to diseases has been significantly enhanced. Summary of the invention
[0005] The purpose of the present invention is to provide a biological synergistic fungicide composition in the form of an aqueous solution or a suspension as a product dosage form in view of the current extreme shortage of highly effective and broad-spectrum pesticides for diseases and the various deficiencies of existing single pesticide formulations.
[0006] The technical solution for achieving the above-mentioned purpose is: a synergistic biofungicide composition containing Bacillus licheniformis, comprising an active ingredient, an adjuvant and a filler, characterized in that: the active ingredient is Bacillus licheniformis and Bacillus cereus, the Bacillus licheniformis accounts for 2-20% of the total mass of the composition, the Bacillus cereus accounts for 1-30% of the total mass of the composition, the adjuvant accounts for 2-25% of the total mass of the composition, and the filler accounts for 25-95% of the total mass of the composition.
[0007] Furthermore, the Bacillus licheniformis accounts for 10-20% of the total mass of the composition, and the Bacillus cereus accounts for 1-2% of the total mass of the composition.
[0008] Furthermore, the composition is in the form of an aqueous solution or a suspension.
[0009] Furthermore, when the composition is in the form of an aqueous solution, the auxiliary agent is an emulsifier.
[0010] Furthermore, when the composition adopts a suspending agent, the auxiliary agents include a dispersing wetting agent, an antifreeze agent, a thickener and a defoaming agent, wherein the dispersing wetting agent accounts for 1 to 15% of the total mass of the composition, the antifreeze agent accounts for 0.3 to 3% of the total mass of the composition, the thickener accounts for 0.4 to 5% of the total mass of the composition, and the defoaming agent accounts for 0.3 to 2% of the total mass of the composition.
[0011] Another object of the present invention is to provide a method for preparing the above product: First, Bacillus licheniformis, Bacillus cereus, adjuvants and fillers are fully mixed in an enamel stirring kettle, and then a suspending agent ground by a sand mill is added, and then mixed to obtain a finished product. The invention combines Bacillus licheniformis and Bacillus cereus into a biological pesticide with low toxicity, low residue, broad bactericidal spectrum, obvious synergy, good prevention and control effect, and reduced prevention and control cost. It is an ideal high-efficiency and low-toxic pesticide preparation variety for preventing and controlling diseases of major economic crops.
[0012] The synergistic biofungicide composition of the invention has a strong disease prevention and treatment effect on agricultural diseases, and can be used to prevent and treat a variety of diseases such as cash crops. The fungicide composition is different from other existing fungicide compositions or pesticide single agents. It adopts a reasonable combination of two biological pesticides, Bacillus licheniformis and Bacillus cereus, which can delay the development of disease resistance. It is simple and safe to use, has a good prevention and control effect, has a low residue and is safe for the environment, promotes the safety and high-quality production of crops, and plays an important role in increasing agricultural production and income.
[0013] The formulation of the biofungicide composition has many advantages such as simple production, high efficiency, low preparation cost, good environmental compatibility, etc. The biofungicide composition is different from other existing fungicide compositions and can delay the generation of drug resistance, thereby achieving the purpose of increasing agricultural production and income and ensuring food safety.
[0014] In order to improve the control effect, when feeding, the Bacillus licheniformis accounts for 10-20% of the total feeding mass of the composition, and the Bacillus cereus accounts for 1-2% of the total feeding mass of the composition. DETAILED DESCRIPTION
[0015] The present invention will be further described below in conjunction with examples. The following examples are only used to illustrate the present invention but not to limit the present invention.
[0016] Embodiment 1: To prepare 10% Bacillus licheniformis and Bacillus cereus aqueous solution, the percentage of each raw material in the total input mass is: Bacillus licheniformis 3% Bacillus cereus 7% TX-10 (emulsifier) 10% Deionized water (filler) 80% The materials are weighed according to the above weight ratio, and each material is added in sequence under stirring. After being stirred and mixed evenly, a product is obtained. The product is tested for field efficacy in preventing and controlling cucumber downy mildew. The efficacy is good, and the prevention efficiency of watermelon downy mildew is 80%.
[0017] Table 1: Effectiveness of 10% Bacillus licheniformis and Bacillus cereus aqueous solution against cucumber downy mildew 10 days after application (%)
[0018] The above test results show that 10% Bacillus licheniformis and Bacillus cereus aqueous solution has a prevention effect of more than 72% on cucumber downy mildew, and has the significant advantage of pollution-free biological control, which is conducive to agricultural promotion and application.
[0019] Embodiment 2: To prepare 20% Bacillus licheniformis·Bacillus cereus suspension, the percentage of each raw material in the total feed weight is: Bacillus licheniformis 5% Sintraxin 15% Nonylphenol polyoxyethylene ether (NP-9) (dispersing wetting agent) 8% Propylene glycol (antifreeze) 1% Xanthan gum (thickener) 1% Silicone defoamer (SAG612) 2% Water (filler) 68% The materials were weighed according to the above weight ratio, stirred and mixed evenly, and then sand-milled by a sand mill to obtain the product, which was then used for field efficacy test on the prevention and treatment of cucumber downy mildew.
[0020] Table 2: The efficacy of 20% Bacillus licheniformis and Bacillus cereus suspension on cucumber downy mildew 10 days after application (%)
[0021] The above test results show that the 20% Bacillus licheniformis·Bacillus cereus suspension has a control effect of more than 80% on cucumber downy mildew, and has the significant advantage of pollution-free biological control, which is conducive to agricultural promotion and application.
Claims
1. A synergistic biofungicide composition containing Bacillus licheniformis, comprising an active ingredient, an adjuvant and a filler, characterized in that The active ingredients are Bacillus licheniformis and Bacillus cereus, wherein the Bacillus licheniformis accounts for 2-20% of the total mass of the composition, the Bacillus cereus accounts for 1-30% of the total mass of the composition, the auxiliary agent accounts for 2-25% of the total mass of the composition, and the filler accounts for 25-95% of the total mass of the composition.
2. The composition according to claim 1, characterized in that: The Bacillus licheniformis accounts for 10-20% of the total mass of the composition, and the Bacillus cereus accounts for 1-2% of the total mass of the composition.
3. The composition according to claim 1, characterized in that: The dosage form of the composition is aqueous solution or suspension.
4. The composition according to claim 3, characterized in that: When the composition is in the form of an aqueous solution, the auxiliary agent is an emulsifier.
5. The composition according to claim 3, characterized in that: When the composition adopts a suspending agent, the auxiliary agents include a dispersing wetting agent, an antifreezing agent, a thickener and a defoaming agent, wherein the dispersing wetting agent accounts for 1 to 15% of the total mass of the composition, the antifreezing agent accounts for 0.3 to 3% of the total mass of the composition, the thickener accounts for 0.4 to 5% of the total mass of the composition, and the defoaming agent accounts for 0.3 to 2% of the total mass of the composition.
6. The method for preparing a synergistic fungicide composition containing Bacillus licheniformis according to claim 1, characterized in that: Firstly, Bacillus licheniformis, Bacillus cereus, auxiliary agent and filler are fully mixed in an enameled stirring kettle, and then the suspending agent ground by a sand mill is added, and then mixed evenly to obtain a finished product.