Acoustic tag implanting device and method for reducing stress response of large yellow croaker

Abstract

The invention relates to the technical field of fish behaviors and acoustic telemetering, in particular to an acoustic tag implanting device and method for reducing stress response of large yellow croaker. A fish body fixing device for fixing a fish body is arranged above a position between the anesthesia water tank and the fish body postoperative rest-culture tank; the interior of the anesthesia water tank is divided into a fish body adaptation temporary rearing area and a flow making anesthetic feeding area through a first partition plate which is longitudinally arranged, wherein the fish body adaptation temporary rearing area and the flow making anesthetic feeding area can achieve water flow circulation, and high-definition cameras are installed above the anesthesia water tank and the fish body postoperation convalescent rearing tank. According to the device and the method, the working efficiency of minimally invasive surgery, acoustic tag implantation and suture surgery can be improved, and the influence of operation stress on the large yellow croaker is effectively reduced.

Description

Technical Field

[0001] The invention relates to the technical field of fish behavior and acoustic telemetry, and in particular to an acoustic tag implantation device and method for reducing stress response of large yellow croaker. Background Art

[0002] Acoustic telemetry (AT) is the main method for obtaining movement and behavior data of aquatic animals worldwide. Acoustic telemetry can track and monitor aquatic animals. Acoustic telemetry has a wide range of applicability. It is one of the most promising technologies for studying global ecological and management issues. It can provide important data information for aquaculture. At the same time, it can provide data support for fisheries and fish habitat management. Stress is a non-specific physiological response of organisms when facing environmental stimuli. As a defensive mechanism, it aims to help the body better adapt to environmental changes. However, when the intensity of the stimulus imposed by the stressor exceeds the scope of the body's regulatory and adaptive capacity, it may cause an imbalance in the body's internal environment, thereby inducing the occurrence of diseases or aggravating the development process and deterioration trend of existing diseases. In aquaculture production practice, operations such as capture, sewage discharge, and transportation may cause certain pressure on fish, thereby triggering stress responses in farmed fish. Fish catching and anesthesia operations, fish minimally invasive surgery, and acoustic tag implantation are no exception.

[0003] Large yellow croaker (Larimichthys crocea) belongs to the family Sciaenidae. Compared with most fish, it is easily disturbed by the outside world and produces stress reactions. This stress reaction often causes abnormal behavior of large yellow croaker or changes in physiological and biochemical indicators. Severe stress reactions of large yellow croaker may even cause collisions with aquaculture tanks or aquaculture cages, resulting in injuries to the jaws, chins, fins, etc. of large yellow croaker, leading to the death of large yellow croaker. In addition, the abnormal behavior of individual large yellow croakers is also likely to cause group reactions, affecting the overall aquaculture quality. In recent years, large yellow croaker aquaculture, as an important economic fish species in my country, has gradually moved to the deep sea. The behavioral data of large yellow croaker in deep-sea cages has important reference value for the welfare aquaculture and precision aquaculture of large yellow croaker. In order to ensure the accuracy of the behavioral data of large yellow croaker in deep-sea cages, reducing the stress reaction of large yellow croaker acoustic tags during implantation is an urgent problem to be solved. Therefore, in view of the above problems, an acoustic tag implantation device that reduces the stress response of large yellow croaker is designed, and a method for implanting large yellow croaker acoustic tags is proposed. Summary of the invention

[0004] The purpose of the present invention is to provide an acoustic tag implantation device and method for reducing the stress response of large yellow croaker. The device can improve the working efficiency of minimally invasive surgery, acoustic tag implantation and suture surgery, and effectively reduce the impact of operational stress on large yellow croaker.

[0005] The technical solution of the present invention is: an acoustic tag implantation device for reducing the stress response of large yellow croaker, comprising an anesthesia water tank, a fish post-operative rest tank is arranged on one side of the anesthesia water tank, and a fish fixing device for fixing the fish is arranged above the anesthesia water tank and the fish post-operative rest tank; the anesthesia water tank is divided into a fish adaptation temporary resting area and a flow-generating anesthetic delivery area by a first partition arranged longitudinally, and high-definition cameras are installed above the anesthesia water tank and the fish post-operative rest tank.

[0006] Furthermore, the first partition is a black opaque partition, and both sides of the first partition are provided with a plurality of holes for enabling fish bodies to adapt to water circulation in the temporary holding area and the flow-generating anesthetic delivery area.

[0007] Furthermore, a baffle connected to the first baffle is transversely arranged in the fish adaptation temporary breeding area, and a scale line for measuring the volume of seawater is arranged on the outer side wall of the fish adaptation temporary breeding area.

[0008] Furthermore, a flow-generating pump, a first oxygenation pump and a funnel with a lower end extending into the flow-generating anesthetic agent delivery area are arranged in one end of the flow-generating anesthetic agent delivery area, and a water outlet is arranged at the other end of the flow-generating anesthetic agent delivery area.

[0009] Furthermore, the fish body fixing device includes two L-shaped steel plates, a sponge pad is connected between the upper ends of the vertical plates of the L-shaped steel plates, a pair of spring screws are passed through the lower parts of the vertical plates of the L-shaped steel plates, and nuts are screwed on the ends of the spring screws passing through the vertical plates.

[0010] Furthermore, a windproof and dustproof cover is provided on the outer side cover of the fish body fixing device, and a lighting lamp with a lighting direction facing downward is provided on the top of the windproof and dustproof cover.

[0011] Furthermore, the fish body resting tank after surgery is divided into left and right areas by a second partition arranged longitudinally, and the left and right areas are divided into multiple breeding areas by transverse partitions arranged along the longitudinal intervals, and the second partition and the transverse partition are both provided with holes.

[0012] Furthermore, a second oxygen pump is provided in the fish body post-operative resting tank, and a drain outlet is provided at one end of the fish body post-operative resting tank.

[0013] A method for implanting an acoustic tag for reducing the stress response of a large yellow croaker includes an acoustic tag implanting device for reducing the stress response of a large yellow croaker, and includes the following steps: (1) Before transferring the large yellow croaker, turn on the flow pump and the first aeration pump to ensure that the water body has sufficient dissolved oxygen; (2) The large yellow croaker used for anesthesia is transferred to the fish adaptation temporary holding area of ​​the anesthesia tank; (3) After the large yellow croaker has adapted to the temporary holding area for 2 days, anesthetics are placed in the flow-generating anesthetic placement area to allow the anesthetics to be evenly mixed with the water flow; (4) The anesthesia state of the yellow croaker is determined by a high-definition camera. When the yellow croaker enters the deep anesthesia period, the yellow croaker is quickly transferred to a fish fixing device and the acoustic tag is implanted; (5) Quickly transfer the large yellow croaker to a post-operative rest tank and start the oxygen pump in the tank in advance to ensure that the large yellow croaker can recover quickly in oxygen-rich water after surgery.

[0014] Furthermore, the water temperature in the anesthesia water tank is 20°C and the salinity is 22.

[0015] Compared with the prior art, the present invention has the following advantages: 1. The device is fully functional and can refine every step of the entire process of acoustic tag implantation in large yellow croaker, optimize anesthesia, minimally invasive surgery, suturing, and postoperative rest and recovery during acoustic tag implantation, and the "V"-shaped adjustable fish fixation device can adjust the spacing according to different fish sizes to better fix the fish body, and can omit the step of fishing and transferring during the anesthesia of large yellow croaker, thereby minimizing the impact of human operation coercion on large yellow croaker, effectively reducing the stress response of large yellow croaker, and improving the work efficiency of minimally invasive surgery, acoustic tag implantation, and suturing surgery.

[0016] 2. This method conducts experiments from multiple angles such as anesthesia dosage, anesthesia method, and fish fixation, and proposes a systematic method for implanting acoustic tags in large yellow croaker. Different fish have different sensitivities to stress, and similarly, the degree of damage caused by the stress they suffer also varies. Since large yellow croaker is quite sensitive and intense to the stress of mechanical operation, the device and method proposed in the present invention can avoid the stress response of large yellow croaker caused by operational stress. Therefore, the present invention can be applied and promoted to the research on acoustic tag implantation of more fish species, and can provide technical support for related research on acoustic telemetry of fish. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the structure of the anesthesia water tank of the present invention; Figure 3 It is a schematic diagram of the fish body fixing device of the present invention; Figure 4 This is a schematic diagram of the structure of the fish resting area after surgery of the present invention; Figure 5 It is a schematic diagram of a water tank partition of the present invention; Figure 6 This is a schematic diagram of the anesthesia state of large yellow croaker of the present invention; Figure 7 It is a heat map of the activity of the anesthesia process of large yellow croaker of the present invention; Figure 8 This is a flowchart of the implantation of acoustic tags for large yellow croaker of the present invention; Fig. 9 This is a diagram showing an example of wound suturing of a large yellow croaker according to the present invention; In the figure: 1-anesthesia water tank, 2-fish fixing device, 3-fish postoperative resting tank, 4-flow anesthetic delivery area, 5-fish adaptation temporary holding area, 6-first partition, 7-baffle, 8-hole, 9-water outlet, 10 flow pump, 11-first oxygen pump, 12-funnel, 13-scale line, 14-high-definition camera, 15-wind and dustproof cover, 16-lighting lamp, 17-sponge pad, 18-spring screw, 19-nut, 20-L-shaped steel plate, 21-rectangular water tank, 22-second oxygen pump, 23-second partition, 24-transverse partition, 25-drainage outlet, 26-high-definition camera. DETAILED DESCRIPTION

[0018] In order to make the above features and advantages of the present invention more easily understood, embodiments are given below with reference to the accompanying drawings for detailed description, but the present invention is not limited thereto.

[0019] refer to Figures 1 to 9 An acoustic tag implantation device for reducing the stress response of large yellow croaker comprises an anesthesia water tank 1, a fish postoperative resting tank 3 is arranged on one side of the anesthesia water tank 1, and a fish body fixing device 2 for fixing the fish body is arranged above the anesthesia water tank 1 and the fish postoperative resting tank 3; the anesthesia water tank 1 is divided into a fish body adaptation temporary resting area 5 and a flow-generating anesthetic delivery area 4 by a first partition 6 arranged longitudinally, and a high-definition camera 14 and a high-definition camera 26 are installed above the anesthesia water tank 1 and the fish postoperative resting tank 3. The behavior state changes of the large yellow croaker during the anesthesia process are observed by the high-definition camera 14, and the postoperative state of the large yellow croaker is monitored in real time by the high-definition camera 26.

[0020] In this embodiment, the first partition 6 is a black opaque partition, and nine holes 8 are arranged at intervals on both sides of the first partition 6 to enable fish bodies to adapt to water circulation in the temporary breeding area 5 and the flow-generating anesthetic delivery area 4.

[0021] In this embodiment, a baffle plate 7 connected to the first baffle plate 6 is disposed transversely in the fish adaptation temporary breeding area 5 to change the flow direction and block a certain amount of water flow. A scale line 13 for measuring the volume of seawater is disposed on the outer wall of the fish adaptation temporary breeding area 5.

[0022] In this embodiment, a flow pump 10, a first oxygen pump 11 and a funnel 12 with the lower end extending into the flow anesthetic agent delivery area are arranged in one end of the flow anesthetic agent delivery area 4, and a water outlet 9 is arranged at the other end of the flow anesthetic agent delivery area 4.

[0023] In this embodiment, the power of the flow pump 10 is 20 W and the flow rate is 20000 L / h. The water outlet 9 can be used to install a circulating water system or a faucet for wastewater discharge according to actual needs.

[0024] In this embodiment, the fish body fixing device includes two L-shaped steel plates 20, a sponge pad 17 is connected between the upper ends of the vertical plates of the L-shaped steel plates 20, and a pair of spring screws 18 are inserted between the lower parts of the vertical plates of the L-shaped steel plates 20, and the ends of the spring screws 18 passing through the vertical plates are screwed with nuts 19. The spacing between the L-shaped steel plates 20 can be adjusted by screwing the nuts 18 according to actual needs, and finally the sponge pad 17 will form a "V"-shaped groove for fixing the fish body.

[0025] In this embodiment, a windproof and dustproof cover 15 is provided on the outer side of the fish body fixing device 2, and a lighting lamp 16 with a lighting direction facing downward is provided on the top of the windproof and dustproof cover 15.

[0026] In this embodiment, the fish resting tank after surgery is a rectangular water tank 21. The fish resting tank after surgery 3 is divided into left and right areas by a second partition 23 arranged longitudinally, and the left and right areas are divided into multiple breeding areas by transverse partitions 24 arranged longitudinally at intervals, and the second partition 23 and the transverse partition 24 are both provided with holes.

[0027] In this embodiment, the fish body postoperative resting tank 3 is provided with a second oxygen pump 22 extending into the left and right areas respectively, and a drain 25 is provided at one end of the fish body postoperative resting tank 3. The drain 25 is used to discharge water, and a circulating water system can also be installed according to actual conditions.

[0028] A method for implanting an acoustic tag for reducing the stress response of a large yellow croaker includes an acoustic tag implanting device for reducing the stress response of a large yellow croaker, and includes the following steps: (1) Before transferring the large yellow croaker, turn on the flow pump and the first aeration pump to ensure that the water has sufficient dissolved oxygen and the flow pump operates normally; (2) To avoid fishing transfer stress, the large yellow croaker used for anesthesia was transferred to the fish adaptation temporary holding area of ​​the anesthesia tank; (3) After the large yellow croaker has adapted to the temporary holding area for 2 days, anesthetics are placed in the flow-generating anesthetic placement area to allow the anesthetics to be evenly mixed with the water flow; (4) The anesthesia state of the yellow croaker is determined by a high-definition camera. When the yellow croaker enters the deep anesthesia period, the yellow croaker is quickly transferred to a fish fixing device and the acoustic tag is implanted; (5) Quickly transfer the large yellow croaker to a post-operative rest tank and start the oxygen pump in the tank in advance to ensure that the large yellow croaker can recover quickly in oxygen-rich water after surgery.

[0029] Example 1 Specifically, step one: the large yellow croaker can be anesthetized 2 days after entering the adaptation and temporary holding area. The large yellow croaker used for implanting the acoustic tag has a total length of 26.11±1.24 cm, a weight of 261.13±10.08 g, a water temperature of 20°C, and a salinity of 22. The anesthetic MS-222 is selected, and the optimal concentration for anesthesia is 120 mg / L. The weighed MS-222 is slowly added to the flow-making anesthetic delivery area 4 through a funnel, and the MS-222 is evenly mixed in the water flow generated by the flow-making pump 10. During the MS-222 addition process, the operator did not appear in the field of vision of the large yellow croaker to avoid scaring it and causing a stress response in the large yellow croaker. The large yellow croaker was not stimulated by fishing, and its state was stable in the adaptation and temporary holding area 5. Through behavioral analysis, the large yellow croaker did not show any stress response during the entire anesthesia process, and it was located in the right area of ​​the tank, such as Figure 7 .

[0030] The generated water flows through the holes on the first partition 6 and enters the fish adaptation temporary rearing area 5. The anesthesia state of the yellow croaker is determined by the high-definition camera (14), and the anesthesia stage is divided into VI stages. When the high-definition camera 14 detects that the yellow croaker has entered the VI stage of deep anesthesia ( Figure 6 , Table 1), then quickly transfer the large yellow croaker to the fish body fixing device 2.

[0031] Table 1 The stages of anesthesia and their corresponding behavioral characteristics of large yellow croaker .

[0032] Step 2: After the yellow croaker is anesthetized and fixed, use the same anesthetic concentration of MS-222 (120 mg / L) to rinse the fixed yellow croaker gills. Use a medical cotton swab to apply iodine disinfectant ( Figure 8 A). The forceps, scalpels, suture needles, sutures, acoustic tags, etc. used in the operation should be rinsed with diluted disinfectant (iodine disinfectant: water = 1: 9) to avoid infection of the fish.

[0033] Use a No. 24 scalpel to scrape off the scales in the abdominal cavity of the yellow croaker to facilitate incising the abdominal epidermis and subsequent suturing. After scraping off the scales, use a No. 12 scalpel to make an incision of about 0.5 cm from top to bottom along the middle of the abdominal cavity, and then use sterilized curved medical scissors to expand the incision from bottom to top to 1.5 cm (the size of the incision is selected according to the size of the implanted acoustic tag) ( Figure 8 B).

[0034] Step 3: Use sterile medical tweezers to remove the sterilized acoustic tag and implant it into the fish's abdominal cavity at an angle of about 30°. Figure 8 C) If a certain sense of obstruction occurs during the implantation process, the implantation angle of the tag should be adjusted appropriately to ensure that the implantation process does not cause damage to the fish's internal organs.

[0035] Step 4: After the tag is implanted ( Figure 8 D) Suturing needs to be done quickly. Sutures are available in absorbable and non-absorbable varieties. In this case, non-absorbable surgical sutures are used, model: 4-0. The suture needle is a semicircular needle, model: 1 / 2 4×14 (needle tip is round; arc is 1 / 2 arc; Φ=0.4 mm, distance from needle tip to needle tail = 14 mm) ( Figure 8 E).

[0036] Use a sterilized needle holder to clamp the middle and rear 1 / 3 of the suture needle, insert the needle into the epidermis on the right side of the wound on the large yellow croaker body, use sterilized medical tweezers to assist the suture needle to completely pass through the right side of the epidermis, and then pass through the left side of the wound. Use tweezers and the needle holder to complete the first surgical knot.

[0037] The suturing method used is the simple interrupted method, and the knotting method is the surgical knot (each circle is wrapped twice to increase friction and prevent it from loosening when the next circle is tied. Each surgical knot is tied 3 times in total). A total of 3 stitches are required in the abdominal cavity of the yellow croaker ( Fig. 9 A) Avoid open stitches at the suture site, which may cause infection in the wound and lead to the death of the fish. After suturing, apply erythromycin ointment to the wound of the yellow croaker.

[0038] Step 5: The sutured large yellow croaker is quickly transferred to the postoperative rest area 3, and the second oxygen pump 22 in the postoperative rest area is turned on in advance to ensure that the large yellow croaker can recover quickly in the oxygen-rich water. The state of the large yellow croaker is observed by a camera during the entire rest process to reduce the stress of the large yellow croaker caused by human interference and the cracking of the fish wound.

[0039] Embodiment 2 This embodiment is consistent with some of the operating steps of the first embodiment, including: fish anesthesia, fish minimally invasive surgery, acoustic tag implantation, fish resuscitation and rest, that is, step one, step two, step three and step five are the same as the first embodiment.

[0040] The difference is that: compared with Example 1, Step 4 of Example 2 also sutures the abdominal cavity of the fish. The suturing method selected in this embodiment is to use a disposable skin stapler to suture the fish wound. Compared with Example 1, the disposable skin stapler can complete the suturing of the fish wound more quickly, greatly shortening the surgical suturing time. However, the staples are made of metal. After the large yellow croaker is put into the net cage or the sea, long-term acoustic monitoring is prone to rust of the staples, causing infection or death of the fish. Therefore, the disposable skin stapler is suitable for the acoustic tag implantation wound suture surgery for short-term large yellow croaker behavior monitoring research.

[0041] After disassembling the disposable skin stapler (DSS-W-35, China), the diluted disinfectant (iodine disinfectant: water = 1: 9) was sprayed on the exit of the stapler to reduce the chance of infection at the wound site of the large yellow croaker.

[0042] Use sterilized tweezers to adjust the wound epidermis of the yellow croaker to ensure that the staples are not crooked. After the first staple is inserted into the wound epidermis, quickly disinfect the exit of the staple with diluted disinfectant (iodine disinfectant: water = 1: 9) and complete the insertion of the second staple. After disinfecting the exit of the staple again, complete the insertion of the third staple ( Fig. 9 B).

[0043] After the suturing is completed, the remaining steps are the same as those in the first embodiment.

[0044] The above description is only a preferred embodiment of the present invention. For ordinary technicians in this field, according to the teachings of the present invention, designing different forms of acoustic tag implantation devices and methods for reducing the stress response of large yellow croaker does not require creative labor. Without departing from the principles and spirit of the present invention, all equal changes, modifications, substitutions and variations made within the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. An acoustic tag implantation device for reducing the stress response of large yellow croaker, comprising an anesthesia water tank, characterized in that: A fish postoperative rest tank is arranged on one side of the anesthesia water tank, and a fish fixing device for fixing the fish is arranged above the anesthesia water tank and the fish postoperative rest tank; the anesthesia water tank is divided into a fish adaptation temporary rest area and a flow-generating anesthetic delivery area by a first partition arranged longitudinally to realize water circulation, and high-definition cameras are installed above the anesthesia water tank and the fish postoperative rest tank.

2. The acoustic tag implantation device for reducing the stress response of large yellow croaker according to claim 1, characterized in that: The first partition is a black opaque partition, and both sides of the first partition are provided with a plurality of holes for enabling fish bodies to adapt to water circulation in the temporary rearing area and the flow-generating anesthetic delivery area.

3. The acoustic tag implantation device for reducing the stress response of large yellow croaker according to claim 1 or 2, characterized in that: A baffle connected to the first baffle is transversely arranged in the fish adaptation temporary breeding area, and a scale line for measuring the volume of seawater is arranged on the outer side wall of the fish adaptation temporary breeding area.

4. The acoustic tag implantation device for reducing the stress response of large yellow croaker according to claim 1, characterized in that: A flow pump, a first oxygenation pump and a funnel with a lower end extending into the flow anesthetic delivery area are arranged in one end of the flow anesthetic delivery area, and a water outlet is arranged at the other end of the flow anesthetic delivery area.

5. An acoustic tag implantation device for reducing stress response of large yellow croaker according to claim 1, 2 or 4, characterized in that: The fish body fixing device includes two L-shaped steel plates, a sponge pad is connected between the upper ends of the vertical plates of the L-shaped steel plates, a pair of spring screws are passed through the lower parts of the vertical plates of the L-shaped steel plates, and nuts are screwed on the ends of the spring screws passing through the vertical plates.

6. The acoustic tag implantation device for reducing the stress response of large yellow croaker according to claim 5, characterized in that: A windproof and dustproof cover is provided on the outer side cover of the fish body fixing device, and a lighting lamp with a lighting direction facing downward is provided on the top of the windproof and dustproof cover.

7. An acoustic tag implantation device for reducing stress response of large yellow croaker according to claim 1, 2, 4 or 6, characterized in that: The fish resting tank after surgery is divided into left and right areas by a second partition arranged longitudinally, and the left and right areas are divided into multiple breeding areas by transverse partitions arranged along the longitudinal intervals, and the second partition and the transverse partition are both provided with holes.

8. The acoustic tag implantation device for reducing the stress response of large yellow croaker according to claim 7, characterized in that: A second oxygenation pump is arranged in the fish body postoperative resting tank, and a drainage port is arranged at one end of the fish body postoperative resting tank.

9. A method for implanting an acoustic tag to reduce the stress response of large yellow croaker, comprising the acoustic tag implanting device to reduce the stress response of large yellow croaker according to claim 4, characterized in that: The following steps are involved: (1) Before transferring the large yellow croaker, turn on the flow pump and the first aeration pump to ensure that the water body has sufficient dissolved oxygen; (2) The large yellow croaker used for anesthesia is transferred to the fish adaptation temporary holding area of ​​the anesthesia tank; (3) After the large yellow croaker has adapted to the temporary holding area for 2 days, anesthetics are placed in the flow-generating anesthetic placement area to allow the anesthetics to be evenly mixed with the water flow; (4) The anesthesia state of the yellow croaker is determined by a high-definition camera. When the yellow croaker enters the deep anesthesia period, the yellow croaker is quickly transferred to a fish fixing device and the acoustic tag is implanted; (5) Quickly transfer the large yellow croaker to a post-operative rest tank and start the oxygen pump in the tank in advance to ensure that the large yellow croaker can recover quickly in oxygen-rich water after surgery.

10. The method for implanting an acoustic tag to reduce the stress response of large yellow croaker according to claim 9, characterized in that: The water temperature in the anesthesia water tank is 20°C and the salinity is 22.

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