Composite extract, preparation method thereof and anti-aging skin care product

Abstract

The invention particularly relates to a composite extract, a preparation method thereof and an anti-aging skin care product. The compound extract is prepared from the following raw materials in parts by weight: 12 to 18 parts of olive leaf extract, 7 to 13 parts of herba patriniae extract, 25 to 35 parts of green tea extract, 7 to 13 parts of inula flower extract and 17 to 23 parts of fructus xanthii extract. The anti-aging skin care product comprises the following raw materials in percentage by mass: 1-5% of a functional agent, 0.1-0.3% of a thickening agent, 3-9% of a composite extract, 5-15% of an emulsifier, 0.1-0.5% of a preservative, 0.05-0.1% of essence and the balance of water. The composite extract has good anti-oxidation effect, water retention performance and storage performance, and endows the skin care product with a good skin care effect; the anti-aging skin care product is added with various plant extract components, can cooperate with the Sirtuins protein activator to act together, has good moisturizing, anti-wrinkle and anti-aging effects, and can be stably stored at normal temperature.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a composite extract and a preparation method thereof, and an anti-aging skin care product. Background Art

[0002] Anti-aging is an enduring hot topic in the cosmetics industry. With the continuous penetration of the concept of "healthy aging", the consumer group of anti-aging products has gradually expanded to younger ages. According to survey data, among the Chinese people who are concerned about anti-aging, 80.2% have started using anti-aging skin care products before the age of 35. At present, the anti-aging products of foreign brands have a long development time and strong brand effect, and have certain technical and brand advantages; while domestic brands have also paid attention to this consumer market, continuously laid out the anti-aging track, and have won the favor of some consumers, and are gradually entering the high-end market. According to the statistical analysis of the current research results, in the field of cosmetics, there are few cases involving the compound use of plant extracts and sirtuins protein activating functional substances. Therefore, the present invention aims to form the two into an organic whole and realize their anti-aging effects from multiple paths and aspects.

[0003] Sirtuins are a class of deacetylases, belonging to a family of proteins that regulate cell health. They can regulate human metabolism. Their mechanism of action is mainly through the efficient activation of SIRT1 by resveratrol, which works with NAD+ coenzyme to activate Sirtuins protein. Sirtuins protein regulates gene transcription to promote cell metabolism and play an antioxidant role. Nicotinamide can also significantly increase NAD+ coenzyme. By increasing the NAD+ content, it can stimulate the activity of Sirtuins protein to achieve the purpose of anti-aging. However, most Sirtuins proteins are only used in clinical research, and their application research in daily chemicals has not been carried out in depth. Sirtuins, as a natural protein in the human body, has the functions of delaying aging, controlling inflammation, and repairing DNA oxidative damage to a certain extent. It can regulate and control cell death pathways and turn on and off aging genes. Therefore, the application of Sirtuins protein activation ingredients combined with plant extracts in the field of cosmetics has certain potential value for the development of future functional skin care products. Summary of the invention

[0004] In order to overcome the shortcomings and deficiencies in the prior art, the purpose of the present invention is to provide a composite extract and a preparation method thereof; the composite extract has good antioxidant effect, water retention and storage properties, small toxic and side effects, and can be used in various skin care cosmetics to give skin care products good skin care effects; the preparation method thereof has a simple process, high production efficiency, and is conducive to industrial production.

[0005] Another object of the present invention is to provide the application of a composite extract in anti-aging skin care products, anti-aging skin care products and their preparation methods. The anti-aging skin care products are added with the above-mentioned composite extracts, which contain multiple plant extract ingredients and work synergistically with Sirtuins protein activators, and have good moisturizing, anti-wrinkle and anti-aging effects. The preparation method is simple in steps and operation, and the prepared cosmetics have good stability.

[0006] The purpose of the present invention is achieved through the following technical scheme: a composite extract, comprising the following raw materials in parts by weight: 12-18 parts of olive leaf extract, 7-13 parts of Patrinia extract, 25-35 parts of green tea extract, 7-13 parts of Inula flower extract, and 17-23 parts of Xanthium sibiricum extract.

[0007] The composite extract of the present invention is compounded with plant extracts such as olive leaf extract, Patrinia extract, green tea extract, Inula extract, and Xanthium sibiricum extract to obtain a composite extract. The plant extract, as a natural raw material, has the advantages of small toxic and side effects and low irritation. The tea polyphenols, flavonoids and other ingredients contained in the plant raw materials selected by the present invention can directly remove active oxygen free radicals, have an antioxidant effect, can nourish cell function, and have excellent water retention and storage properties, and can be used as a cosmetic efficacy raw material. The composite extract has the advantages of mild efficacy, small side effects, wide adaptability, and low cost as a pure natural plant composition, which conforms to the healthy skin care concept of "natural, green, and safe". The raw materials cooperate with each other and work synergistically to produce an effect of 1+1>2. The composite extract works synergistically with the sirtuins protein activator and can be used for various skin care cosmetics, and different dosages can be added according to the type and needs of the preparation.

[0008] Furthermore, the preparation method of the olive leaf extract comprises the following steps:

[0009] A1. Weigh olive leaf raw material, soak it in ethanol solvent method, heat and reflux after soaking, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrate;

[0010] A2. The concentrate is adsorbed and eluted by a macroporous adsorption resin, first eluted with deionized water, discarded, and then eluted with an ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain an olive leaf extract.

[0011] Furthermore, the preparation method of the Patrinia scabra extract comprises the following steps:

[0012] B1, weighing the raw material of Patrinia patriniae, heating and refluxing extraction by ethanol solvent method, collecting the extract, filtering and centrifuging, and then concentrating to 3-6 times of the original mass to obtain a concentrated solution;

[0013] B2. The concentrate is adsorbed and eluted by macroporous adsorption resin, first eluted with water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain the Patrinia scabra extract.

[0014] Furthermore, the preparation method of the green tea extract comprises the following steps:

[0015] C1. Weigh the high mountain green tea raw material, crush and sieve it, and then use a supercritical carbon dioxide extractor to extract it. First, put the green tea powder into the reactor, set the extraction pressure to 10.0-40.0Mpa, the CO2 flow rate to 3-9L / h, the extraction temperature to 25-55°C, balance for 30-60min, extraction time to 1-4h, and the entrainer to be a 30%-80% concentration ethanol solution;

[0016] C2. After the extraction is completed, the extract is collected from the separation kettle, and then the ethanol is removed by reduced pressure distillation to obtain a high mountain green tea extract sample.

[0017] Furthermore, the preparation method of the Inula flower extract comprises the following steps:

[0018] D1. Weigh the raw material of Inula japonica, extract it by heating and refluxing with ethanol solvent method, collect the extract, filter and centrifuge, and then concentrate it to 3-5 times of the original mass to obtain a concentrated solution;

[0019] D2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with an ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 3-6 times the mass of the raw material to obtain the Inula japonica extract.

[0020] Furthermore, the preparation method of the Xanthium sibiricum extract comprises the following steps:

[0021] E1. Weigh the Xanthium sibiricum raw material, extract it by ultrasonic-ethanol extraction method, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrated solution;

[0022] E2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and the eluate is concentrated under reduced pressure to 2-5 times the mass of the raw material.

[0023] The present invention also provides a method for preparing a composite extract, comprising the following steps: weighing olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract, and Xanthium sibiricum extract in proportion, mixing them evenly, and obtaining the composite extract.

[0024] Another object of the present invention is achieved through the following technical scheme: an anti-aging skin care product comprises the following raw materials in percentage by mass: 1-5% of efficacy agent, 0.1-0.3% of thickener, 3-9% of composite extract, 5-15% of emulsifier, 0.1-0.5% of preservative, 0.05-0.1% of flavor, and the balance is water.

[0025] Furthermore, the efficacy agent includes nicotinamide and at least one of retinol, ceramide, glutathione, dipotassium glycyrrhizinate and tranexamic acid. The anti-aging skin care product provided by the present invention uses nicotinamide as a Sirtuins protein activator, and is compounded with other raw materials to form an efficacy agent, and the efficacy agent is combined with plant extracts to obtain a skin care cosmetic rich in multiple plant extracts and synergistic Sirtuins protein activators. The cosmetic has the advantages of simple preparation, moisturizing, anti-wrinkle and anti-aging.

[0026] Furthermore, the thickener is at least one of carbomer and xanthan gum.

[0027] Furthermore, the emulsifier is at least one of carnosine, sodium hyaluronate, glycerol, 1,2-hexanediol and sodium pyrrolidonecarboxylate.

[0028] Furthermore, the preservative is at least one of p-hydroxybenzophenone, phenoxyethanol and caprylhydroxamic acid.

[0029] In the present invention, a method for preparing an anti-aging skin care product comprises the following steps:

[0030] S1, weigh a thickener and a portion of water, mix, stir and dissolve, obtain a mixture a, set aside;

[0031] S2, weigh the efficacy agent and part of the water, mix them, stir and dissolve them, and obtain a mixture b, which is set aside;

[0032] S3, weighing the composite extract and setting aside;

[0033] S4, weighing an emulsifier and a portion of water, mixing, stirring evenly, obtaining a mixture C, and setting aside;

[0034] S5. Weigh the preservatives and flavors and set aside;

[0035] S6, adding mixture b to mixture a, stirring evenly, slowly adding the composite extract, and adjusting the pH, after fully mixing evenly, adding mixture c, stirring evenly, adding preservatives and essence, and then shearing and emulsifying, discharging after passing the test, and obtaining an anti-aging skin care product;

[0036] Among them, steps S1-S5 can be performed simultaneously or in a reversed order.

[0037] The beneficial effects of the present invention are as follows: the composite extract of the present invention is prepared by compounding plant extracts such as olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract, and Xanthium sibiricum extract, and has the advantages of small toxic and side effects, small irritation, etc., and has good antioxidant effect, water retention performance and storage performance, and can be used in various skin care cosmetics to give skin care products good skin care effects. The composite extract of the present invention and the sirtuins protein activator synergistically can be used in various skin care cosmetics, and different amounts can be added according to the type and needs of the preparation. The preparation method thereof is simple in process, high in production efficiency, and is conducive to industrial production.

[0038] The anti-aging skin care product using the above-mentioned composite extract of the present invention contains multiple plant extract ingredients, which can work synergistically with the Sirtuins protein activator to have good moisturizing, anti-wrinkle and anti-aging effects. The preparation method is simple to operate, and the prepared cosmetics are in good condition and can be stably stored at room temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a test curve of skin moisture content when evaluating the moisturizing effect of the anti-aging skin care product of Example 4.

[0040] Figure 2 This is a test curve chart of skin water loss when evaluating the moisturizing effect of the anti-aging skin care product of Example 4.

[0041] Figure 3 This is a test curve chart of the number of skin wrinkles when the anti-aging skin care product of Example 4 is evaluated for anti-wrinkle. DETAILED DESCRIPTION

[0042] In order to facilitate the understanding of those skilled in the art, the present invention is further described below in conjunction with embodiments and drawings. The contents mentioned in the implementation modes are not intended to limit the present invention.

[0043] In some embodiments of the present invention, a composite extract comprises the following raw materials in parts by weight: 12-18 parts of olive leaf extract, 7-13 parts of Patrinia scabra extract, 25-35 parts of green tea extract, 7-13 parts of Inula japonica extract, and 17-23 parts of Xanthium sibiricum extract.

[0044] In some embodiments of the present invention, the method for preparing the olive leaf extract comprises the following steps:

[0045] A1. Weigh the olive leaf raw material, soak it in ethanol solvent method for 40-90min, heat it to reflux after soaking, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrate; the extraction conditions are: the extraction solvent is 75-85% ethanol aqueous solution, the solid-liquid ratio is 1:8-12 (g / mL), the extraction temperature is 78-82°C, the extraction time is 1.5-3h, and the number of extractions is 2-4 times; the olive leaf raw material is wild fresh dry raw material;

[0046] A2, the concentrate is adsorbed and eluted by macroporous adsorption resin, first eluted with deionized water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain olive leaf extract. The flavonoid content in the olive leaf extract is ≥1.5mg / g.

[0047] Furthermore, in step A2, the resin is an AB-8 macroporous adsorption resin, which is first activated with 90-96% ethanol for 22-26 hours, and then washed with 2.5-3.5 column volumes of deionized water for standby use.

[0048] Furthermore, in step A2, the elution conditions of the macroporous adsorption resin are: adsorption time is 45-75min, the elution solvent is 65-75% ethanol aqueous solution, and the elution rate is 1-3s / d; the concentration conditions are: heating temperature is 45-65°C, condensation temperature is -10 to -6°C, rotation speed is 70-90rpm, and pressure is 150-210hpa.

[0049] In some embodiments of the present invention, the method for preparing the Patrinia chinensis extract comprises the following steps:

[0050] B1, weighing the raw material of Patrinia patriniae, adopting ethanol solvent method to heat reflux extraction, collecting the extract, filtering and centrifuging, and then concentrating to 3-6 times of the original mass to obtain a concentrate; the extraction conditions are: the extraction solvent is 45-60% ethanol aqueous solution, the solid-liquid ratio is 1:18-22 (g / mL), the extraction temperature is 75-85°C, the extraction time is 1.5-3h, and the number of extractions is 2-4 times; the raw material of Patrinia patriniae is a wild fresh dry raw material;

[0051] B2, the concentrate is adsorbed and eluted by macroporous adsorption resin, first eluted with water, discarded, then eluted with ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain the Patrinia extract. The flavonoid content in the Patrinia extract is ≥1.2mg / g.

[0052] Furthermore, in step B2, the resin is an AB-8 macroporous adsorption resin, which is first activated with 90-96% ethanol for 22-26 hours, and then washed with 2.5-3.5 column volumes of deionized water for standby use.

[0053] Furthermore, in step B2, the elution conditions of the macroporous adsorption resin are: adsorption time is 45-75min, the elution solvent is 55-65% ethanol aqueous solution, and the elution rate is 1-4s / d; the concentration conditions are: heating temperature is 45-65°C, condensation temperature is -10 to -6°C, rotation speed is 70-90rpm, and pressure is 150-210hpa.

[0054] In some embodiments of the present invention, the method for preparing the green tea extract comprises the following steps:

[0055] C1. Weigh the high mountain green tea raw material, crush and sieve it, and then use a supercritical carbon dioxide extractor to extract it; the supercritical extraction steps are: first, put the green tea powder into a reactor, set the extraction pressure to 10.0-40.0Mpa, the CO2 flow rate to 3-9L / h, the extraction temperature to 25-55°C, balance for 30-60min, extraction time to 1-4h, and the entrainer to be a 30-80% concentration ethanol solution; the green tea raw material is a dry raw material of high mountain green tea

[0056] C2. After the extraction is completed, the extract is collected from the separation kettle, and then the ethanol is removed by reduced pressure distillation to obtain a high mountain green tea extract sample;

[0057] In the embodiment of the present invention, the tea polyphenol content in the green tea extract is calculated using an ultraviolet spectrophotometer, and the tea polyphenol content in the green tea extract is ≥85 mg / g.

[0058] Furthermore, the preparation method of the Inula flower extract comprises the following steps:

[0059] D1. Weigh the raw material of Inula japonica, extract it by heating and refluxing with an ethanol solvent method, collect the extract, filter and centrifuge, and then concentrate it to 3-5 times of the original mass to obtain a concentrated solution; the raw material of Inula japonica is a dry raw material of fresh flowers; the extraction conditions are: the extraction solvent is 65-75% ethanol aqueous solution, the solid-liquid ratio is 1:31 (g / mL), the extraction temperature is 75-85°C, the extraction time is 0.5-1h, and the number of extractions is 2-4 times; the raw material of Inula japonica is a dry raw material of fresh flowers;

[0060] D2, the concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, then eluted with ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 3-6 times the mass of the raw material to obtain the Inula japonica extract. The flavonoids content in the Inula japonica extract is ≥1.5 mg / g.

[0061] Furthermore, in step D2, the resin is a D101 macroporous adsorption resin, which is first activated with 90-96% ethanol for 22-26 hours, and then washed with 2.5-3.5 column volumes of deionized water for standby use.

[0062] Furthermore, in step D2, the elution conditions of the macroporous adsorption resin are: adsorption time is 45-75min, the elution solvent is 35-45% ethanol aqueous solution, and the elution rate is 1-3s / d; the concentration conditions are: heating temperature is 45-65°C, condensation temperature is -10 to -6°C, rotation speed is 70-90rpm, and pressure is 120-200hpa.

[0063] In some embodiments of the present invention, the method for preparing the Xanthium sibiricum extract comprises the following steps:

[0064] E1. Weigh the Xanthium sibiricum raw material, extract it by ultrasonic-ethanol extraction method, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrate; the Xanthium sibiricum raw material is a wild thorn-free dry raw material;

[0065] E2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and the eluate is concentrated under reduced pressure to 2-5 times the mass of the raw material.

[0066] Furthermore, in step E1, the extraction conditions are: accurately weigh the raw materials, crush them and place them in a container, use 50-60% ethanol for ultrasonic extraction, the ultrasonic power is 100-500W, the solid-liquid ratio is 1:15-19 (g / mL), the extraction temperature is 30-60°C, the extraction time is 30-70min, the number of extractions is 1-3 times, and filtering is performed while hot.

[0067] Furthermore, in step E2, the resin is a D101 macroporous adsorption resin, which is first activated with 90-96% ethanol for 22-26 hours, and then washed with 2.5-3.5 column volumes of deionized water for standby use.

[0068] Furthermore, in step E2, the elution conditions of the macroporous adsorption resin are: adsorption time is 45-75min, the elution solvent is 35-45% ethanol aqueous solution, and the elution rate is 1-3s / d; the concentration conditions are: heating temperature is 45-65°C, condensation temperature is -10 to -6°C, rotation speed is 70-90rpm, and pressure is 120-200hpa.

[0069] In some embodiments of the present invention, a method for preparing a composite extract comprises the following steps: weighing olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract, and Xanthium sibiricum extract in proportion, mixing them evenly, and obtaining a composite extract.

[0070] In some embodiments of the present invention, an anti-aging skin care product comprises the following raw materials in percentage by weight: 1-5% of an efficacy agent, 0.1-0.3% of a thickener, 3-9% of a composite extract, 5-15% of an emulsifier, 0.1-0.5% of a preservative, 0.05-0.1% of a flavor, and the remainder is water.

[0071] In some embodiments of the present invention, the efficacy agent is at least one of niacinamide, retinol, ceramide, glutathione, dipotassium glycyrrhizinate and tranexamic acid.

[0072] In some embodiments of the present invention, the thickener is at least one of carbomer and xanthan gum.

[0073] In some embodiments of the present invention, the composite extract is at least one of olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract and Xanthium sibiricum extract.

[0074] In some embodiments of the present invention, the emulsifier is at least one of carnosine, sodium hyaluronate, glycerol, 1,2-hexanediol, and sodium pyrrolidonecarboxylate.

[0075] In some embodiments of the present invention, the preservative is at least one of p-hydroxybenzophenone, phenoxyethanol and 10% caprylhydroxamic acid.

[0076] In some embodiments of the present invention, a method for preparing an anti-aging skin care product comprises the following steps:

[0077] S1. Weigh the thickener and part of the water, mix them, stir and dissolve them at 24-30°C to obtain a mixture a, which is set aside;

[0078] S2, weigh the efficacy agent and part of the water, mix them, stir and dissolve them at 55-65°C to obtain a mixture b, which is set aside;

[0079] S3, weighing the composite extract and setting aside;

[0080] S4, weigh the emulsifier and part of the water, mix them, stir them evenly at a temperature of 35-45° C. to obtain a mixture c, which is set aside;

[0081] S5. Weigh the preservatives and flavors and set aside;

[0082] S6, adding mixture b to mixture a, stirring evenly, slowly adding the composite extract, and adjusting the pH to 5-7, after fully mixing, adding mixture c, stirring evenly, adding preservatives and essence, and then shearing and emulsifying, discharging after passing the test, and obtaining an anti-aging skin care product;

[0083] Among them, steps S1-S5 can be performed simultaneously or in a reversed order.

[0084] In some embodiments of the present invention, in step S6, the mixture b is added to a, and the mixture is quickly stirred evenly with a magnetic stirrer at a stirring speed of 500-1000 rpm and a temperature of 30-50°C for 20-50 min; the composite extract is slowly added at room temperature, and the pH is adjusted. After being fully mixed, the mixture c is added at 20-40°C, stirred evenly, and then preservatives and flavors are added. The mixture is sheared and emulsified at 7000-10000 rpm with a high-speed shearing machine for 5-25 min, and the material is discharged after passing the test.

[0085] Example 1

[0086] In this embodiment, a composite extract includes the following raw materials in parts by weight: the following raw materials in parts by weight: 15 parts of olive leaf extract, 10 parts of Patrinia scabra extract, 30 parts of green tea extract, 10 parts of Inula japonica extract, and 20 parts of Xanthium sibiricum extract.

[0087] Furthermore, the preparation method of the olive leaf extract comprises the following steps:

[0088] A1. Weigh 20g of purchased olive leaves and soak them in 80% ethanol solution at a solid-liquid ratio of 1:10 (g / mL) for 1h. Then heat and reflux for extraction at 80°C for 2h, extract twice under the same conditions, filter and centrifuge, concentrate to 5 times the original mass, recover the solvent, and obtain a crude olive leaf extract, which is stored in a refrigerator for later use;

[0089] A2, AB-8 resin was soaked in 95% ethanol for 24h, wet loaded, rinsed with 3 times column volume of 95% ethanol, the effluent was free of white turbidity, and then rinsed with water until there was no alcohol taste. Take olive leaf extract in AB-8 resin at a flow rate of 1s / d, adsorb on the sample for 1h, purify, elute with 800mL of distilled water to remove impurities, and then elute with 800mL of 70% ethanol solution to obtain eluent, after complete elution, the eluent was concentrated under reduced pressure by a rotary evaporator at 180hpa, condensation temperature -8°C, water bath heating 60°C, 90rpm, and the eluent was concentrated under reduced pressure to 3.1 times the mass of the raw material, and the flavonoid content in the concentrated sample was determined using a multifunctional microplate reader, and the flavonoid content in the olive leaf extract was 1.87mg / g.

[0090] Furthermore, the preparation method of the Patrinia scabra extract comprises the following steps:

[0091] B1, the purchased Patrinia scabra was weighed 15g, with 50% ethanol solution, according to the solid-liquid ratio of 1:20 (g / mL), heated under reflux extraction at 80 ° C for 2h, extracted twice under the same conditions, filtered and centrifuged, concentrated to 4.5 times the original mass, recovered the solvent, and obtained a crude extract of Patrinia scabra, which was stored in a refrigerator for standby use;

[0092] B2. Soak the AB-8 resin in 95% ethanol for 24 hours, wet-pack the column, and rinse with 3 times the column volume of 95% ethanol. The effluent is free of white turbidity and then rinsed with water until there is no alcohol taste. Take the Patrinia extract in the AB-8 resin at a flow rate of 1s / d, adsorb on the sample for 1h, and then purify it. Elute with 1400mL of distilled water to remove impurities, and then elute with 700mL of 60% ethanol solution to obtain an eluate. After complete elution, the eluate is concentrated under reduced pressure on a rotary evaporator at 180hpa, condensation temperature -8°C, water bath heating at 55°C, and 90rpm. The eluate is concentrated under reduced pressure to 4.7 times the mass of the raw material. The flavonoid content in the concentrated sample is determined using a multifunctional microplate reader, and the flavonoid content in the Patrinia extract is measured to be 1.42mg / g.

[0093] Furthermore, the preparation method of the green tea extract comprises the following steps:

[0094] C1. Accurately weigh 25.0g of high mountain green tea raw material and crush it through a 40-mesh sieve. Use a supercritical carbon dioxide extractor for extraction. First, put the green tea powder into the reactor, set the extraction pressure to 27.0Mpa, the CO2 flow rate to 7.5L / h, the extraction temperature to 47°C, balance for 50min, supercritical extraction for 2.5h, and the entrainer to be a 65% ethanol solution;

[0095] C2. After the extraction is completed, the extract is collected from the separation kettle, and then the ethanol is distilled off under reduced pressure to obtain a high mountain green tea extract sample. Finally, the tea polyphenol content is calculated using an ultraviolet spectrophotometer, and the tea polyphenol content in the green tea extract is determined to be 114 mg / g.

[0096] Furthermore, the preparation method of the Inula japonica extract comprises the following steps:

[0097] D1. Weigh 20 g of the purchased Inula japonica flower, use 70% ethanol solution, according to the solid-liquid ratio of 1:31 (g / mL), heat and reflux extraction at 80°C for 40 min, extract twice under the same conditions, collect the filtrate and centrifuge at 5000 rpm and 25°C for 15 min, concentrate to 5 times the original mass, recover the solvent, and obtain the crude extract of Inula japonica flower, which is stored in the refrigerator for later use;

[0098] D2. Soak AB-8 resin in 95% ethanol for 24 hours, wet-pack the column, rinse with 3 times the column volume of 95% ethanol, and the effluent is free of white turbidity. Rinse with water until there is no alcohol taste. Take the extract of Inula japonica in AB-8 resin at a flow rate of 1s / d, load and adsorb for 1h, elute with 1000mL of water to remove impurities, and then elute with 600mL of 70% ethanol to obtain an eluent. After complete elution, the eluent is concentrated under reduced pressure by a rotary evaporator at 200hpa, condensation temperature -8°C, water bath heating 56°C, and 90rpm. The eluent is concentrated under reduced pressure to 3.2 times the mass of the raw material. The flavonoid content in the concentrated sample is determined using a multifunctional microplate reader. The flavonoid content in the Inula japonica extract is determined to be 1.83mg / g.

[0099] Furthermore, the preparation method of the Xanthium sibiricum extract comprises the following steps:

[0100] E1. Weigh 20 g of the purchased Xanthium sibiricum, use 55% ethanol solution, at a solid-liquid ratio of 1:17 (g / mL), ultrasonically extract at 50°C for 30 min, set the ultrasonic power to 450 W, extract once, filter and centrifuge at 6000 rpm and 25°C for 15 min, concentrate to 5 times the original mass, recover the solvent, and obtain a crude extract of Xanthium sibiricum, which is stored in a refrigerator for later use.

[0101] E2. Take D101 resin and soak it in 95% ethanol for 24 hours, wet-pack the column, rinse it with 3 times the column volume of 95% ethanol, and the effluent is free of white turbidity. Rinse it with water until there is no alcohol taste. Take the Xanthium sibiricum extract in D101 resin at a flow rate of 1s / d, load it and adsorb it for 1h, elute it with 800mL of water to remove impurities, and then elute it with 400mL of 40% ethanol to obtain the eluent. After complete elution, it is concentrated under reduced pressure by a rotary evaporator at 140hpa, condensation temperature -8℃, water bath heating 56℃, and 80rpm. The eluent is concentrated under reduced pressure to 3.2 times the mass of the raw material. The flavonoid content in the concentrated sample is determined using a multifunctional microplate reader. The flavonoid content in the Xanthium sibiricum extract is 2.27mg / g.

[0102] In this embodiment, the preparation method of the composite extract comprises the following steps: olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract, and Xanthium sibiricum extract are weighed in proportion, mixed evenly, and the composite extract is obtained.

[0103] Example 2

[0104] In this embodiment, a composite extract includes the following raw materials in parts by weight: 12 parts of olive leaf extract, 7 parts of Patrinia scabra extract, 25 parts of green tea extract, 7 parts of Inula japonica extract, and 17 parts of Xanthium sibiricum extract.

[0105] The rest of the content of this embodiment is the same as that of Embodiment 1.

[0106] Example 3

[0107] In this embodiment, a composite extract includes the following raw materials in parts by weight: 18 parts of olive leaf extract, 13 parts of Patrinia scabra extract, 35 parts of green tea extract, 13 parts of Inula japonica extract, and 23 parts of Xanthium sibiricum extract.

[0108] The rest of the content of this embodiment is the same as that of Embodiment 1.

[0109] Example 4

[0110] This embodiment provides an anti-aging skin care product, which includes the following raw materials in percentage by mass: 1.85% of efficacy agent, 0.3% of thickener, 8.5% of composite extract, 11.33% of emulsifier, 0.1% of preservative, 0.05% of essence, and the balance is water. The composite extract is the composite extract prepared in Example 1. The preservative is phenoxyethanol.

[0111] Furthermore, the thickener comprises the following raw materials in parts by weight: 0.2 parts of xanthan gum and 0.1 parts of carbomer.

[0112] Furthermore, the efficacy agent includes the following raw materials in parts by weight: 0.1 part of dipotassium glycyrrhizinate, 0.5 part of glutathione, 0.1 part of niacinamide, 0.1 part of ceramide, 0.05 part of retinol, and 1 part of tranexamic acid. In this embodiment, the efficacy agent is prepared by compounding the above raw materials, and the Sirtuins protein activator and the composite extract act synergistically, so that the anti-aging skin care product has good moisturizing, anti-wrinkle and anti-aging effects.

[0113] Furthermore, the emulsifier includes the following raw materials in parts by weight: 0.03 parts of disodium EDTA, 0.1 parts of sodium hyaluronate, 5 parts of glycerol, 2 parts of 1,2-hexanediol, 2 parts of sodium pyrrolidonecarboxylate, 1 part of carnosine, 1 part of hydrogenated castor oil, and 0.2 parts of laurocapram.

[0114] In this embodiment, the method for producing anti-aging skin care products comprises the following steps:

[0115] S1: Accurately weigh xanthan gum, carbomer and 20 g of distilled water, mix them, and stir and dissolve them at 25°C for 2 h to obtain a mixture a for later use;

[0116] S2: Accurately weigh nicotinamide, glutathione, tranexamic acid, dipotassium glycyrrhizinate, ceramide, and retinol, respectively, and mix with 5 g of distilled water, and stir and dissolve at 60°C for 15 min to obtain a mixture B for later use;

[0117] S3: Accurately weigh the composite extract and set aside;

[0118] S4: Accurately weigh glycerol, 1,2-hexanediol, sodium pyrrolidone carboxylate, carnosine, disodium EDTA, and azolam, respectively, and mix with 1 g of distilled water, and stir evenly at 40° C. to obtain a mixture c, which is set aside;

[0119] S5: Accurately weigh preservatives and flavors and set aside;

[0120] S6: Add mixture b to mixture a, stir quickly and evenly with a magnetic stirrer at a speed of 1000 rpm and a temperature of 50°C, and keep warm for 20 minutes; then slowly add the composite extract at room temperature, adjust the pH to 6.5, and after fully mixing, add mixture c at 30°C, stir evenly, add preservatives and flavors, and homogenize and emulsify at 9400 rpm for 12 minutes. Discharge the sample after it passes the test.

[0121] In this embodiment, in the method for preparing the anti-aging skin care product, the raw materials and dosage ratios added in each step are shown in Table 1 below:

[0122] Table 1

[0123]

[0124]

[0125] Example 5

[0126] This embodiment provides an anti-aging skin care product, which includes the following raw materials in percentage by mass: 1.5% of efficacy agent, 0.3% of thickener, 8.5% of composite extract, 10.5% of emulsifier, 0.1% of preservative, 0.05% of essence, and the balance is water. The composite extract is the composite extract prepared in Example 1. The preservative is phenoxyethanol.

[0127] Furthermore, the thickener comprises the following raw materials in parts by weight: 0.15 parts of xanthan gum and 0.15 parts of carbomer.

[0128] Furthermore, the efficacy agent includes the following raw materials in parts by weight: 0.1 part of dipotassium glycyrrhizinate, 0.5 part of glutathione, 0.15 part of niacinamide, 0.15 part of ceramide, 0.1 part of retinol, and 0.5 part of tranexamic acid.

[0129] Furthermore, the emulsifier includes the following raw materials in parts by weight: 0.04 parts of disodium EDTA, 0.16 parts of sodium hyaluronate, 4 parts of glycerol, 2 parts of 1,2-hexanediol, 2 parts of sodium pyrrolidonecarboxylate, 1.1 parts of carnosine, 1 part of hydrogenated castor oil, and 0.2 parts of laurocapram.

[0130] In this embodiment, the method for producing anti-aging skin care products comprises the following steps:

[0131] S1: Accurately weigh xanthan gum, carbomer and 20 g of distilled water, mix them, and stir and dissolve them at 25°C for 2 h to obtain a mixture a for later use;

[0132] S2: Accurately weigh nicotinamide, glutathione, tranexamic acid, dipotassium glycyrrhizinate, ceramide, and retinol, respectively, and mix with 5 g of distilled water, and stir and dissolve at 60°C for 15 min to obtain a mixture B for later use;

[0133] S3: Accurately weigh the composite extract and set aside;

[0134] S4: Accurately weigh glycerol, 1,2-hexanediol, sodium pyrrolidone carboxylate, carnosine, disodium EDTA, and azolam, respectively, and mix with 1 g of distilled water, and stir evenly at 40° C. to obtain a mixture c, which is set aside;

[0135] S5: Accurately weigh preservatives and flavors and set aside;

[0136] S6: Add mixture b to mixture a, stir quickly and evenly with a magnetic stirrer at a speed of 1000 rpm and a temperature of 50°C, and keep warm for 20 minutes; then slowly add the composite extract at room temperature, adjust the pH, and mix thoroughly. Then add mixture c at 30°C, stir evenly, add preservatives and flavors, and homogenize and emulsify at 9400 rpm for 12 minutes. Discharge the sample after it passes the test.

[0137] Example 6

[0138] The efficacy of the anti-aging skin care product added with the composite extract and the sirtuins protein activator of Example 4 was evaluated and analyzed:

[0139] (1) Moisturizing effect evaluation:

[0140] To explore the effect of the product on the test results of skin moisture content and skin moisture loss, the German CK multifunctional skin tester was used for testing.

[0141] Preparation before the experiment:

[0142] The test site was cleaned and the test area was marked, where area 1 was the sample group, area 2 was the control group without the extract, area 3 was the control group without the sirtuins protein activator, and area 4 was the blank control group.

[0143] Before the test, sit quietly in a room with a room temperature of 24.5℃ and a relative humidity of 63% for 30 minutes. Do not drink water or beverages, stay relaxed, and avoid touching the test area. The test results are as follows: Figure 1-2 As shown:

[0144] ① Test the skin moisture content and moisture loss values ​​of the four areas before the experiment begins. Repeat the test 3 times and take the average value.

[0145] ② According to the time gradient design, the skin moisture content and moisture loss values ​​of each area in different time periods were tested. Each group of values ​​was tested 3 times and the average value was taken.

[0146] From the results, we can see that anti-aging skin care products with added compound extracts and sirtuins protein activators have the best moisturizing effect, with the highest skin moisture retention rate and the lowest moisture loss, achieving a synergistic effect.

[0147] (2) Anti-wrinkle evaluation:

[0148] Through skin texture testing, the product's improvement on skin wrinkles was explored. The German CK VC98 tester was used to select the SEw parameter, which is positively correlated with the degree of skin wrinkles.

[0149] Preparation before the experiment:

[0150] The test site was cleaned and the test area was marked, where area 1 was the sample group, area 2 was the control group without the extract, area 3 was the control group without the sirtuins protein activator, and area 4 was the blank control group.

[0151] Before the test, sit quietly in a room with a room temperature of 25.2℃ and a relative humidity of 61% for 30 minutes. Do not drink water or beverages, stay relaxed, and avoid touching the test area. The test results are as follows: Figure 3 As shown:

[0152] ① Before the start of the experiment, the SEw values ​​of the skin surface in the four areas were tested. Each area was tested 3 times and the average value was taken.

[0153] ② According to the time gradient design, the changes in SEw values ​​of various regions in different time periods were tested. Each group of values ​​was tested 3 times and the average value was taken.

[0154] From the results, we can see that as time goes by, the skin wrinkles in zone 1 are the least, which is significantly better than the other three control groups and has the best effect. That is, the anti-aging skin care products with added compound extracts and sirtuins protein activators have good anti-wrinkle function, indicating that the formula design is superior and effective.

[0155] Example 7

[0156] The German CK skin gloss test probe GL200 was used to analyze and test the skin gloss and skin friction value. The test results are shown in Table 2 below:

[0157] Table 2

[0158]

[0159]

[0160] Example 8

[0161] The German CK multifunctional skin tester was used to grade the skin brightness and color. The test results are shown in Table 3 below:

[0162] Table 3

[0163]

[0164] The control substance is a matrix other than the extract, and the sample is an application product with a composite extract added. The results in the table show that within 180 minutes, the skin of the sample group is brighter and more translucent; according to the conclusion that the larger the ITA° value, the lighter the skin color, and vice versa, the darker the skin color, it is known that the ITA° values ​​of the sample group are all higher than those of the control group, that is, the melanin content in the skin is lower and the skin color is better. The samples in Examples 7-8 all use the anti-aging skin care products in Example 4.

[0165] Example 9

[0166] The stability evaluation and analysis of the anti-aging skin care product added with the composite extract and the sirtuins protein activator in Example 4 was conducted, and the four indicators of the appearance, consistency, and state of the test product at different temperatures and the changes of the product after centrifugation at 3000r / min for 30min were evaluated and analyzed. The results are shown in Table 4 below:

[0167] Table 4 Stability evaluation table of anti-aging skin care products

[0168]

[0169] From the results in the table, it can be seen that the anti-aging skin care product of the present invention, which is added with the composite extract and the sirtuins protein activator, has good stability, complies with the cosmetic stability test specifications, and can be used as a functional skin care product.

[0170] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention may also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. A composite extract, characterized in that: The invention comprises the following raw materials in parts by weight: 12-18 parts of olive leaf extract, 7-13 parts of Patrinia scabra extract, 25-35 parts of green tea extract, 7-13 parts of Inula japonica extract and 17-23 parts of Xanthium sibiricum extract.

2. The composite extract according to claim 1, characterized in that: The preparation method of the olive leaf extract comprises the following steps: A1. Weigh olive leaf raw material, soak it in ethanol solvent method, heat and reflux after soaking, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrate; A2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with an ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain the olive leaf extract.

3. The composite extract according to claim 1, characterized in that: The preparation method of the Patrinia scabra extract comprises the following steps: B1, weighing the raw material of Patrinia patriniae, heating and refluxing extraction by ethanol solvent method, collecting the extract, filtering and centrifuging, and then concentrating to 3-6 times of the original mass to obtain a concentrated solution; B2. The concentrate is adsorbed and eluted by macroporous adsorption resin, first eluted with water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 2-6 times the mass of the raw material to obtain the Patrinia scabra extract.

4. The composite extract according to claim 1, characterized in that: The preparation method of the green tea extract comprises the following steps: C1. Weigh the high mountain green tea raw material, crush and sieve it, and then use a supercritical carbon dioxide extractor to extract it. First, put the green tea powder into the reactor, set the extraction pressure to 10.0-40.0Mpa, the CO2 flow rate to 3-9L / h, the extraction temperature to 25-55°C, balance for 30-60min, extraction time to 1-4h, and the entrainer to be a 30%-80% concentration ethanol solution; C2. After the extraction is completed, the extract is collected from the separation kettle, and then the ethanol is removed by reduced pressure distillation to obtain a high mountain green tea extract sample.

5. The composite extract according to claim 1, characterized in that: The preparation method of the Inula japonica extract comprises the following steps: D1. Weigh the raw material of Inula japonica, extract it by heating and refluxing with ethanol solvent method, collect the extract, filter and centrifuge, and then concentrate it to 3-5 times of the original mass to obtain a concentrated solution; D2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with an ethanol aqueous solution, the eluate is collected, and then the eluate is concentrated under reduced pressure to 3-6 times the mass of the raw material to obtain the Inula japonica extract.

6. The composite extract according to claim 1, characterized in that: The preparation method of the Xanthium sibiricum extract comprises the following steps: E1. Weigh the Xanthium sibiricum raw material, extract it by ultrasonic-ethanol extraction method, collect the extract, filter and centrifuge, and then concentrate it to 4-6 times of the original mass to obtain a concentrated solution; E2. The concentrate is adsorbed and eluted with a macroporous adsorption resin, first eluted with water, discarded, and then eluted with ethanol aqueous solution, the eluate is collected, and the eluate is concentrated under reduced pressure to 2-5 times the mass of the raw material.

7. A method for preparing a composite extract as described in any one of 1-6, characterized in that: The method comprises the following steps: weighing olive leaf extract, Patrinia scabra extract, green tea extract, Inula japonica extract and Xanthium sibiricum extract in proportion, mixing them evenly and obtaining a composite extract.

8. An anti-aging skin care product, characterized in that: The anti-aging skin care product comprises the following raw materials in percentage by weight: 1-5% of an efficacy agent, 0.1-0.3% of a thickener, 3-9% of a composite extract, 5-15% of an emulsifier, 0.1-0.5% of a preservative, 0.05-0.1% of an essence, and the balance being water.

9. A method for preparing the anti-aging skin care product according to claim 8, characterized in that: The steps include: S1, weigh a thickener and a portion of water, mix, stir and dissolve, obtain a mixture a, set aside; S2, weigh the efficacy agent and part of the water, mix them, stir and dissolve them, and obtain a mixture b, which is set aside; S3, weighing the composite extract and setting aside; S4, weighing an emulsifier and a portion of water, mixing, and stirring evenly to obtain a mixture c, which is set aside; S5. Weigh the preservatives and flavors and set aside; S6, adding mixture b to mixture a, stirring evenly, slowly adding the composite extract, and adjusting the pH, after fully mixing evenly, adding mixture c, stirring evenly, adding preservatives and essence, and then shearing and emulsifying, discharging after passing the test, and obtaining an anti-aging skin care product; Among them, steps S1-S5 can be performed simultaneously or in a reversed order.

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