Antioxidant composition containing Campanumoea maculata bark extract and application of antioxidant composition containing Campanumoea maculata bark extract

Abstract

The invention discloses an anti-oxidation composition containing a calystegia octovalvis bark extract and application of the anti-oxidation composition, and belongs to the technical field of cosmetics. The anti-oxidation composition containing the Campanumoea maculata bark extract comprises the following components: the Campanumoea maculata bark extract, acetyl hexapeptide-8 and squalane, the anti-oxidation composition has a good anti-oxidation effect through the synergistic effect of the three components, and the anti-oxidation composition obtained by the preparation method disclosed by the invention has the advantages that the anti-oxidation effect is good, and the anti-oxidation effect is good. The absorption by the skin is easier.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an antioxidant composition containing a variegated bellflower bark extract and application thereof. Background Art

[0002] As we age, problems such as skin aging, pigmentation, and wrinkles become increasingly prominent, and free radicals play a key role in this. Free radicals are triggered by environmental factors such as ultraviolet rays, pollution, and stress, leading to oxidative stress, damaging skin cells and tissues, and accelerating skin aging. To resist oxidative damage, antioxidants such as vitamin C, vitamin E, and green tea extract are often added to cosmetics, but these traditional antioxidants have problems such as poor stability and low bioavailability, which limit their effectiveness. The introduction of nanotechnology has brought new opportunities for antioxidant cosmetics. Carrier systems such as nanoemulsions can improve the solubility, stability, and bioavailability of active ingredients and enhance antioxidant effects, but nanoemulsions require high technical processes and are difficult to reduce costs. In addition, existing antioxidant cosmetics contain chemical additives, and the active substances are easily affected by the environment and have a short service life, making it difficult to meet consumers' demand for natural, organic, and chemical-free products. Therefore, the development of natural antioxidants has become a research hotspot and trend in the current cosmetics field. Summary of the invention

[0003] The purpose of the present invention is to provide an antioxidant composition containing a variegated bellflower tree bark extract and an application thereof. The antioxidant composition is compounded with natural plant extracts, acetyl hexapeptide-8 and squalane, has high safety, and has significant antioxidant effects.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions: In a first aspect, the present invention provides an antioxidant composition containing a variegated bellflower tree bark extract, comprising the following components: a variegated bellflower tree bark extract, acetyl hexapeptide-8, and squalane.

[0005] Preferably, the antioxidant composition containing the extract from the bark of the variegated bellflower tree comprises the following components in parts by weight: Extract of the bark of the variegated bellflower tree: 0.05-3 parts; Acetyl hexapeptide-8: 0.01-5 parts; Squalane: 0.01-1 part.

[0006] Further preferably, the antioxidant composition containing the extract from the bark of the variegated bellflower tree comprises the following components in parts by weight: Extract of variegated bellflower tree bark: 2.5-3 parts; Acetyl hexapeptide-8: 1-5 parts; Squalane: 0.2-1 part.

[0007] Most preferably, the antioxidant composition containing the extract from the bark of the variegated bellflower tree comprises the following components in parts by weight: Extract of variegated bellflower tree bark: 2.5 parts; Acetyl hexapeptide-8: 1 part; Squalane: 0.2 parts.

[0008] In a second aspect, the present invention provides a method for preparing the extract of the variegated bellflower tree bark according to the first aspect, the preparation method comprising the following steps: S1. The bark of the variegated bellflower tree is air-dried to a moisture content of ≤5%, and ground into coarse powder; S2. Mixing the crude powder of the bark of the variegated bellflower tree with rapeseed oil and polyglycerol-3 diisostearate, heating the mixture to 50-60° C., and stirring the mixture at 300 rpm for 10-30 min to obtain a mixed solution; S3. Under N2 atmosphere, the mixed solution in S2 was subjected to ultrasonic-assisted extraction to obtain an extract, with extraction temperature of 40–60°C, ultrasonic frequency of 20–40 kHz, and extraction time of 30-60 min; S4. Filtering the extract to remove insoluble matter, centrifuging, and taking the supernatant to obtain a variegated bellflower tree bark extract; Wherein, in step S2, the mass ratio of the crude powder of the variegated bellflower tree bark, rapeseed oil, and polyglycerol-3 diisostearate is 1:1.6-12:0.04-1.2.

[0009] In a third aspect, the present invention provides a facial cream with antioxidant and anti-aging effects, wherein the facial cream with antioxidant and anti-aging effects is composed of the following components in percentage by mass: 1-3 wt % of the antioxidant composition containing the extract from the bark of the variegated bellflower tree of the first aspect; Humectant: 2-10wt%; Emollient: 5-20wt%; Emulsifier: 5-10wt%; Preservative: 0.2-0.5wt%; Thickener: 0.1-1wt%; Deionized water was added to 100 wt%; The moisturizing agent is one or more of glycerin, propylene glycol, and butylene glycol; The emollient is one or more of vaseline, shea butter, isohexadecane, and isopropyl isostearate; The preservative is one or more of methylparaben, sodium benzoate, p-hydroxyacetophenone, and sorbic acid; The emulsifier is one or more of cetearyl alcohol, PEG-100 stearate, and glyceryl stearate.

[0010] The thickener is one or more of xanthan gum, hydroxyethyl cellulose, ammonium acryloyldimethyltaurate / VP copolymer, and sodium polyacryloyldimethyltaurate.

[0011] In a fourth aspect, the present invention provides a method for preparing the facial cream according to the third aspect, the specific steps are as follows: S1: Mix the moisturizer, preservative, thickener and deionized water, heat to 60-80°C and stir at a stirring speed of 200-400 rpm for 1-3 min; then homogenize at a homogenization speed of 5000-7000 rpm for 1-3 min to obtain a mixed solution A; S2: Mix the emollient and the emulsifier, heat to 60-80°C and stir at a stirring speed of 200-400 rpm for 1-3 min; then homogenize at a homogenization speed of 5000-7000 rpm for 1-3 min to obtain a mixed solution B; S3: Add mixed solution B to mixed solution A, stir at a speed of 200-400 rpm for 3-5 min, and then homogenize at a speed of 5000-7000 rpm for 3-5 min to obtain a mixed solution; S4: After the mixed liquid is cooled to 30-50°C, the antioxidant composition is mixed with the mixed liquid, and stirred at a stirring speed of 200-400 rpm for 1-3 min; then homogenized at a homogenization speed of 5000-7000 rpm for 1-3 min, and the temperature is further cooled to 20-25°C to obtain a facial cream with antioxidant and anti-aging effects, which is then filled, sterilized, sealed, and stored at room temperature.

[0012] The antioxidant composition containing the variegated bellflower bark extract of the present invention comprises the variegated bellflower bark extract, acetyl hexapeptide-8, and squalane. Among them, the variegated bellflower bark extract contains polyphenols (such as flavonoids, tannins), polyols and terpenoid compounds, which can directly remove free radicals (ROS, hydroxyl radicals), inhibit lipid peroxidation, and provide electrons to neutralize oxidative active molecules; acetyl hexapeptide-8 can reduce oxidative stress caused by inflammation or stress by inhibiting the release of neurotransmitters (such as catecholamines), and can activate cell signaling pathways, promote collagen synthesis, and repair oxidatively damaged extracellular matrix; squalane can reduce transepidermal water loss and prevent external oxidative factors (such as ozone) from penetrating by simulating the structure of the sebum membrane.

[0013] In the present invention, rapeseed oil containing polyglycerol-3 diisostearate is used to extract the bark of the variegated bellflower tree. By utilizing the similar miscibility of rapeseed oil, fat-soluble active substances such as terpenes (such as triterpenes, sesquiterpenoid compounds), steroids (such as steroidal saponins, steroid hormones), fatty acids (such as fatty acids, fatty oils, etc.), and volatile oils (such as volatile alcohols, alkenes, etc.) in the bark of the variegated bellflower tree can be enriched. In addition, the addition of polyglycerol-3 diisostearate can further improve the biological activity and enrichment rate of the variegated bellflower tree bark extract.

[0014] Compared with the prior art, the beneficial effect of the present invention is that the extract from the bark of the variegated bellflower tree, acetyl hexapeptide-8 and squalane in the present invention are used together to achieve multi-level synergistic synergy.

[0015] First, synergistic antioxidant defense: squalane can form a physical barrier on the surface to reduce the invasion of oxidative stressors while stabilizing the active ingredients; the bark extract of the variegated bellflower tree can quickly remove the generated free radicals and reduce the accumulation of oxidative damage; acetyl hexapeptide-8 can activate endogenous antioxidant enzymes and repair damaged cells, blocking the vicious cycle of oxidation.

[0016] Secondly, synergistic anti-inflammatory and repair: the anti-inflammatory components in the bark extract of the variegated bellflower tree can effectively reduce the release of inflammatory factors, while acetyl hexapeptide-8 inhibits neurogenic inflammation. The two together reduce inflammation-related oxidative stress. At the same time, squalane reduces the penetration of inflammatory factors through barrier repair, forming an "anti-inflammatory-antioxidant" positive cycle. The fat-soluble properties of squalane allow squalane to encapsulate the polar molecules (polyphenols) in the bark extract of the variegated bellflower tree, thereby improving its stability and transdermal absorption rate.

[0017] In summary, the extract from the bark of the variegated bellflower tree, acetyl hexapeptide-8 and squalane in the present invention achieve a synergistic antioxidant effect through a three-dimensional network of "physical barrier-free radical scavenging-endogenous repair", and the carrier function of squalane further amplifies the efficacy to form a highly efficient and long-lasting antioxidant system. DETAILED DESCRIPTION

[0018] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0019] The experimental methods without specific conditions in the following tests are usually carried out under conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0020] Some raw materials and their sources are as follows: The bark of Campanula variegata was purchased from Shanghai Qianfei Chemical Co., Ltd.

[0021] Acetyl hexapeptide-8: purchased from Shanghai Qianfei Chemical Co., Ltd.

[0022] Squalane: purchased from Yichun Sea Turtle Life Science Co., Ltd.

[0023] Rapeseed oil: purchased from Jishui County Hongxing Natural Medicinal Spice Oil Factory.

[0024] Polyglycerol-3 diisostearate: purchased from Guangzhou Aoxue Chemical Co., Ltd.

[0025] Other raw materials and reagents are commercially available.

[0026] The preparation method of the extract from the bark of the variegated bellflower tree of the present invention comprises the following specific steps: Bellflower bark extract1: S1. The bark of the variegated bellflower tree is air-dried to a moisture content of ≤5%, and ground into coarse powder; S2. The crude powder of the bark of the variegated bellflower tree was mixed with rapeseed oil and polyglycerol-3 diisostearate, heated to 55°C, and stirred at 300 rpm for 20 min to obtain a mixed solution; S3. Under N2 atmosphere, the mixed solution in S2 was subjected to ultrasonic-assisted extraction to obtain an extract, with extraction temperature of 55°C, ultrasonic frequency of 30 kHz, and extraction time of 45 min; S4. Filter the extract to remove insoluble matter, centrifuge, and take the supernatant to obtain the variegated bellflower tree bark extract: Wherein, in step S2, the mass ratio of the crude powder of the bark of the variegated campanula tree, rapeseed oil, and polyglycerol-3 diisostearate is 1:3:0.2.

[0027] Variegated Bellflower Bark Extract2: S1. The bark of the variegated bellflower tree is air-dried to a moisture content of ≤5%, and ground into coarse powder; S2. The crude powder of the bark of the variegated bellflower tree was mixed with rapeseed oil and polyglycerol-3 diisostearate, heated to 60°C, and stirred at 300 rpm for 10 min to obtain a mixed solution; S3. Under N2 atmosphere, the mixed solution in S2 was subjected to ultrasonic-assisted extraction to obtain an extract, with extraction temperature of 60°C, ultrasonic frequency of 20 kHz, and extraction time of 60 min; S4. Filter the extract to remove insoluble matter, centrifuge, and take the supernatant to obtain the variegated bellflower tree bark extract: Wherein, in step S2, the mass ratio of the crude powder of the variegated bellflower tree bark, rapeseed oil, and polyglycerol-3 diisostearate is 1:1.6:1.2.

[0028] Bellflower bark extract3: S1. The bark of the variegated bellflower tree is air-dried to a moisture content of ≤5%, and ground into coarse powder; S2. The crude powder of the bark of the variegated bellflower tree was mixed with rapeseed oil and polyglycerol-3 diisostearate, heated to 50 ° C, and stirred at 300 rpm for 30 min to obtain a mixed solution; S3. Under N2 atmosphere, the mixed solution in S2 was subjected to ultrasonic-assisted extraction to obtain an extract, with extraction temperature of 40°C, ultrasonic frequency of 40kHz, and extraction time of 30 min; S4. Filter the extract to remove insoluble matter, centrifuge, and take the supernatant to obtain the variegated bellflower tree bark extract: Wherein, in step S2, the mass ratio of the crude powder of the variegated bellflower tree bark, rapeseed oil, and polyglycerol-3 diisostearate is 1:12:0.04.

[0029] Extract of the bark of the variegated bellflower tree①: The difference from the variegated bellflower bark extract 1 is that an equal amount of water is used to replace the rapeseed oil, and the other steps and parameters are the same as those of the variegated bellflower bark extract 1.

[0030] Extract of the bark of the variegated bellflower tree②: The difference from the variegated bellflower bark extract 1 is that an equal amount of 60 wt % ethanol solution is used to replace rapeseed oil, and the other steps and parameters are the same as those of the variegated bellflower bark extract 1.

[0031] Extract of the bark of the variegated bellflower tree③: The difference from the variegated bellflower bark extract 1 is that polyglyceryl-3 diisostearate is not added in step S2, and the other steps and parameters are the same as those of the variegated bellflower bark extract 1.

[0032] Extract of the bark of the variegated bellflower tree④: Different from the variegated bellflower bark extract 1, the mass ratio of the variegated bellflower bark crude powder, rapeseed oil and polyglycerol-3 diisostearate in step S2 is 1:0.2:3.

[0033] The weight percentages of the components in the antioxidant composition containing the extract from the bark of the variegated bellflower tree of the present invention are as follows: Antioxidant Composition 1: Campanula variegata bark extract (Campanula variegata bark extract 1): 2.5 parts; Acetyl Hexapeptide-8: 1 part; Squalane: 0.2 parts.

[0034] Antioxidant Composition 2: Campanula variegata bark extract (Campanula variegata bark extract 1): 3 parts; Acetyl Hexapeptide-8: 0.01 parts; Squalane: 1 part.

[0035] Antioxidant Composition 3: Campanula variegata bark extract (Campanula variegata bark extract 1): 0.05 parts; Acetyl Hexapeptide-8: 5 parts; Squalane: 0.01 parts.

[0036] Antioxidant composition①: The difference from composition 1 is that the extract of the bark of Campanula variegata is missing, and the missing mass fraction is made up by acetyl hexapeptide-8 and squalane in a mass ratio of 1:0.2.

[0037] Antioxidant composition ②: The difference from composition 1 is that acetyl hexapeptide-8 is missing, and the missing mass portion is made up by the extract of the bark of the genus Campanulata and squalane in a mass ratio of 2.5:0.2.

[0038] Antioxidant composition ③: The difference from composition 1 is that squalane is lacking, and the lacking mass fraction is made up by the extract of the bark of the variegated bellflower tree and acetyl hexapeptide-8 in a mass ratio of 2.5:1.

[0039] Antioxidant composition ④: Extract of the bark of the variegated bellflower tree①: 2.5 parts; acetyl hexapeptide-8: 1 part; squalane: 0.2 parts.

[0040] Antioxidant composition⑤: Extract of the bark of the variegated bellflower tree②: 2.5 parts; acetyl hexapeptide-8: 1 part; squalane: 0.2 parts.

[0041] Antioxidant composition ⑥: Extract of the bark of the variegated bell tree③: 2.5 parts; acetyl hexapeptide-8: 1 part; squalane: 0.2 parts.

[0042] Antioxidant composition ⑦: Extract of the bark of the variegated bellflower tree④: 2.5 parts; acetyl hexapeptide-8: 1 part; squalane: 0.2 parts.

[0043] A facial cream with antioxidant and anti-aging effects, the mass percentages of its components are shown in Table 1 below.

[0044] Table 1 Components and mass percentages of facial creams with antioxidant and anti-aging effects

[0045] Note: the moisturizer is glycerin; the emulsifier is cetearyl alcohol, glyceryl stearate, and PEG-100 stearate, and the mass ratio of cetearyl alcohol, glyceryl stearate, and PEG-100 stearate is 1:1:1; the emollient is shea butter; the preservative is p-hydroxyacetophenone; the thickener is xanthan gum and sodium polyacryloyldimethyl taurate, and the mass ratio of xanthan gum and sodium polyacryloyldimethyl taurate is 2:3.

[0046] The specific steps of the preparation method of the above-mentioned creams 1-5 and creams ①-⑦ are as follows: S1: Mix the moisturizer, preservative, thickener and deionized water, heat to 70°C and stir at a stirring speed of 300 rpm for 2 min; then homogenize at a homogenization speed of 6000 rpm for 2 min to obtain a mixed solution A; S2: Mix the emollient and the emulsifier, heat to 70°C and stir at a stirring speed of 300 rpm for 2 min; then homogenize at a homogenization speed of 6000 rpm for 2 min to obtain a mixed solution B; S3: Add mixed solution B to mixed solution A, stir at a stirring speed of 300 rpm for 5 min, and then homogenize at a homogenization speed of 6000 rpm for 5 min to obtain a mixed solution; S4: After the mixed liquid is cooled to 40°C, the antioxidant composition is mixed with the mixed liquid, and stirred at a stirring speed of 300 rpm for 2 min; then homogenized at a homogenization speed of 6000 rpm for 2 min, and the temperature is further cooled to 25°C to obtain a facial cream with antioxidant and anti-aging effects, which is then filled, sterilized, sealed, and stored at room temperature.

[0047] The preparation method of blank cream is as follows: S1: Mix the moisturizer, preservative, thickener and deionized water, heat to 70°C and stir at a stirring speed of 300 rpm for 2 min; then homogenize at a homogenization speed of 6000 rpm for 2 min to obtain a mixed solution A; S2: Mix the emollient and the emulsifier, heat to 70°C and stir at a stirring speed of 300 rpm for 2 min; then homogenize at a homogenization speed of 6000 rpm for 2 min to obtain a mixed solution B; S3: Add mixed solution B to mixed solution A, stir at a stirring speed of 300 rpm for 5 min, and then homogenize at a homogenization speed of 6000 rpm for 5 min to obtain a mixed solution; S4: The mixed liquid is further cooled to room temperature 40°C and stirred at a stirring speed of 300 rpm for 2 min; then homogenized at a homogenization speed of 6000 rpm for 2 min, and the temperature is further cooled to 25°C to obtain a blank cream, which is then filled, sterilized, sealed, and stored at room temperature.

[0048] Experiment 1: Human safety test

[0049] 1-1. Human skin patch test Test substances: Creams 1-5.

[0050] Subject selection: Healthy volunteers aged 21-57 years old were selected to participate voluntarily and sign the informed consent form. They were able to complete the experiment as required during the test period and were randomly divided into groups with 30 subjects in each group.

[0051] Experimental method: Select qualified patch equipment and closed patch test method, take 1g of the test substance and place it in the patch tester and spread it evenly, then use external low-allergenic tape to apply it to the flexor side of the subject's forearm. Remove the test substance after 24 hours, and observe and record the skin reaction at 0.5, 24, and 48 hours after removal.

[0052] Result evaluation: The classification of adverse skin reactions is determined according to the classification of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The classification standards are shown in Table 2 and the results are shown in Table 3.

[0053] Table 2 Classification criteria for adverse skin reactions Grade classification Phenomenon Level 0 No skin reaction Level 1 Light red spots on the skin Level 2 Skin erythema, infiltration, and papules Level 3 Skin erythema, edema, papules, and blisters Level 4 Skin erythema, edema, and bullae Table 3 Human skin patch test results

[0054] 1-2. Human test on sensitive skin Test substances: Creams 1-5 and Creams ①-⑦.

[0055] Subject selection: healthy volunteers aged 21-44 years old, who were positive in lactic acid sting test, voluntarily participated and signed informed consent, and were able to complete the test as required during the test period were selected. They were randomly divided into groups with 30 subjects in each group.

[0056] Experimental method: Volunteers randomly divided both sides of their faces into test side and control side. After cleansing, the product was evenly applied to the facial skin of the test side according to the product usage instructions, and no product was used on the control side. After 28 days of trial, the volunteers reported local skin reactions. If there were any adverse reactions, dermatologists would evaluate the adverse reactions.

[0057] Result evaluation: The grading of adverse skin reactions is determined according to the grading of adverse skin reactions in human trial tests specified in the "Technical Specifications for Safety of Cosmetics" (2015 edition). The grading standards are shown in Table 1 and the results are shown in Table 4.

[0058] Table 4 Results of human trial test

[0059] According to the results of human skin patch tests, creams 1-5 were safe and had no adverse reactions among the subjects during the test period.

[0060] Judging from the results of the sensitive skin suitability test, during the test period, no subjects experienced adverse skin reactions to Creams 1-5 and Creams ①-⑦, indicating that Creams 1-5 and Creams ①-⑦ are safe, mild and non-irritating, and suitable for people with sensitive skin.

[0061] Test 2: Antioxidant test determination Sample solution: The sample solution is prepared by using anhydrous ethanol to prepare creams 1-5, creams ①-⑦ and blank cream into a mixed solution with a concentration of 5 mg / mL.

[0062] Test steps: 1. Take 2 mL of sample solution and 2 mL of 2×10 -4 1. Mix 1 mol / L DPPH anhydrous ethanol solution (A1). DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is a commonly used free radical scavenging ability test reagent. 2. Take 2mL of anhydrous ethanol and 2mL of 2×10 -4 Mix 1 mol / L DPPH anhydrous ethanol solution (A2); 3. Mix 2 mL of anhydrous ethanol with 2 mL of sample solution (A3); 4. After A1, A2, and A3 have reacted for 40 minutes, use a UV spectrophotometer to detect the absorbance values ​​of A1, A2, and A3 at 517 nm.

[0063] Clearance calculation formula: Clearance (%) = [1-(A1-A3) / A2] × 100% The experimental results are shown in Table 5.

[0064] Table 5 Antioxidant effect

[0065] Sample name Free radical DPPH scavenging rate (%) Face cream 1 <![CDATA[94.59±0.34 * <!-- 9 -->]]> Face Cream 2 <![CDATA[93.67±0.23 * ]]> Cream 3 <![CDATA[94.74±0.14 * ]]> Cream 4 <![CDATA[90.73±0.63 * ]]> Cream 5 <![CDATA[92.14±0.96 * ]]> Facial Cream① <![CDATA[43.62±1.10 *a ]]> Facial Cream② <![CDATA[63.40±0.38 *a ]]> Facial Cream③ <![CDATA[81.25±0.44 *a ]]> Facial Cream④ <![CDATA[68.33±0.71 *a ]]> Facial Cream⑤ <![CDATA[74.81±1.37 *a ]]> Facial Cream ⑥ <![CDATA[87.39±0.15 *a ]]> Facial Cream⑦ <![CDATA[82.74±0.80 *a ]]> Blank facial cream 18.52±0.27 Note: “*” indicates P < 0.05 compared with blank cream; “a” indicates p < 0.05 compared with cream 1.

[0066] Comparing creams 1, 4, and 5, the results show that cream 1 has the best antioxidant effect, so cream 1 is selected as the best embodiment.

[0067] Comparing cream 1 with creams ①-⑦, the results show that the three components of the variegated bellflower tree bark extract, acetyl hexapeptide-8, and squalane provided by the present invention have a significant synergistic effect. Specifically: By comparing cream 1 with creams ①-③, it can be seen that there is a certain synergistic effect between the variegated bellflower tree bark extract, acetyl hexapeptide-8, and squalane in the present invention; By comparing cream 1 with creams ④-⑥, it can be seen that the preparation process of the variegated bellflower tree bark extract in the present invention has an effect on the variegated bellflower tree bark extract and There is a certain influence on the antioxidant effect of the facial cream containing it, among which the choice of extraction solvent will affect the antioxidant effect of the variegated bellflower bark extract and the facial cream containing it, and adding polyglyceryl-3 diisostearate to rapeseed oil can more effectively dissolve the active substances in the variegated bellflower bark, thereby enhancing the antioxidant effect of the variegated bellflower bark extract; by comparing facial cream 1 and facial cream ⑦, it can be seen that polyglyceryl-3 diisostearate and rapeseed oil have a significant synergistic effect in the mass ratio specified in the present invention.

[0068] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An antioxidant composition containing a bark extract of Campanula variegata, characterized in that: The antioxidant composition comprises the following components in parts by weight: Extract of the bark of the variegated bellflower tree: 0.05-3 parts; Acetyl hexapeptide-8: 0.01-5 parts; Squalane: 0.01-1 part; The preparation steps of the variegated bellflower tree bark extract are as follows: S1. The bark of the variegated bellflower tree is air-dried to a moisture content of ≤5%, and ground into coarse powder; S2. Mixing the crude powder of the bark of the variegated bellflower tree with rapeseed oil and polyglycerol-3 diisostearate, heating the mixture to 50-60° C., and stirring the mixture at 300 rpm for 10-30 min to obtain a mixed solution; S3. Under N2 atmosphere, the mixed solution in S2 was subjected to ultrasonic-assisted extraction to obtain an extract, with extraction temperature of 40–60°C, ultrasonic frequency of 20–40 kHz, and extraction time of 30-60 min; S4. Filtering the extract to remove insoluble matter, centrifuging, and taking the supernatant to obtain a variegated bellflower tree bark extract; Wherein, in step S2, the mass ratio of the crude powder of the variegated bellflower tree bark, rapeseed oil, and polyglycerol-3 diisostearate is 1:1.6-12:0.04-1.

2.

2. The antioxidant composition according to claim 1, characterized in that The antioxidant composition comprises the following components in parts by weight: Extract of variegated bellflower tree bark: 2.5-3 parts; Acetyl hexapeptide-8: 1-5 parts; Squalane: 0.2-1 part.

3. Use of the antioxidant composition as claimed in claim 1 or 2 in the preparation of cosmetics with antioxidant and anti-aging effects.

4. The use according to claim 3, characterized in that: The dosage form of the cosmetics with anti-oxidation and anti-aging effects is any one of cream, lotion and essence.

5. A facial cream with anti-oxidation and anti-aging effects, characterized in that: The facial cream comprises 1-3 wt % of the antioxidant composition according to claim 1 or 2.

6. The facial cream with anti-oxidation and anti-aging effects according to claim 5, characterized in that: The facial cream also contains the following components in percentage by weight: Humectant: 2-10wt%; Emollient: 5-20wt%; Emulsifier: 5-10wt%; Preservative: 0.2-0.5wt%; Thickener: 0.1-1wt%; Deionized water was added to make up to 100 wt%.

7. The facial cream according to claim 6, characterized in that The moisturizer is one or more of glycerin, propylene glycol, and butylene glycol; the preservative is one or more of methylparaben, sodium benzoate, p-hydroxyacetophenone, and sorbic acid.

8. The facial cream according to claim 6, characterized in that The emulsifier is one or more of cetearyl alcohol, PEG-100 stearate, and glyceryl stearate.

9. The facial cream according to claim 6, characterized in that The emollient is one or more of vaseline, shea butter, isohexadecane, and isopropyl isostearate; the thickener is one or more of xanthan gum, hydroxyethyl cellulose, ammonium acryloyldimethyltaurate / VP copolymer, and sodium polyacryloyldimethyltaurate.

10. A method for preparing a facial cream as claimed in any one of claims 6 to 9, characterized in that: The following steps are involved: S1: Mix the moisturizer, preservative, thickener and deionized water, heat to 60-80°C and stir at a stirring speed of 200-400 rpm for 1-3 min; then homogenize at a homogenization speed of 5000-7000 rpm for 1-3 min to obtain a mixed solution A; S2: Mix the emollient and the emulsifier, heat to 60-80°C and stir at a stirring speed of 200-400 rpm for 1-3 min; then homogenize at a homogenization speed of 5000-7000 rpm for 1-3 min to obtain a mixed solution B; S3: Add mixed solution B to mixed solution A, stir at a speed of 200-400 rpm for 3-5 min, and then homogenize at a speed of 5000-7000 rpm for 3-5 min to obtain a mixed solution; S4: After the mixed liquid is cooled to 30-50°C, the antioxidant composition is mixed with the mixed liquid, and stirred at a stirring speed of 200-400 rpm for 1-3 min; then homogenized at a homogenization speed of 5000-7000 rpm for 1-3 min, and the temperature is further cooled to 20-25°C to obtain a facial cream with antioxidant and anti-aging effects, which is then filled, sterilized, sealed, and stored at room temperature.

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