Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects and application of ganoderma lucidum and ginseng plant extract composition

Abstract

The invention relates to the field of anti-wrinkle and firming skin care products, in particular to a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects and application of the ganoderma lucidum and ginseng plant extract composition. A lucid ganoderma and ginseng plant extract composition with anti-wrinkle and firming effects comprises the following components in percentage by mass: 15-20% of a ginseng root extract, 3-7% of a roxburgh rose fruit extract, 6-12% of a dendrobium officinale stem extract, 3-7% of a tricholoma matsutake extract, 30-35% of lucid ganoderma yeast fermentation filtrate, a solvent and 0.1-1% of a preservative. The plant extract composition provided by the invention can mildly and efficiently realize the effects of resisting wrinkles, tightening, whitening and the like.

Description

Technical Field

[0001] The present application relates to the field of anti-wrinkle and firming skin care products, and more specifically, to a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects and its application. Background Art

[0002] Skin aging is a natural aging process of the human body, but a variety of internal and external factors can accelerate this process, such as ultraviolet radiation, environmental pollution, and bad living habits. As the skin ages, skin elasticity decreases, and wrinkles and sagging gradually appear, which not only affects the appearance, but may also have a negative impact on the individual's psychological state. Therefore, the development of effective anti-wrinkle and firming functional products is of great significance to delay skin aging and keep the skin healthy and youthful.

[0003] Chemically synthesized ingredients often have specific chemical structures and properties, which may not completely match the physiological structure and function of human skin. Therefore, during use, these ingredients may cause irritation or discomfort to the skin. For example, retinol, phospholipids, and peptides in anti-aging skin care ingredients have significant anti-aging effects, but they are expensive, and retinol is highly irritating and can easily cause skin sensitivity.

[0004] Therefore, there is room for improvement. Summary of the invention

[0005] In order to achieve anti-wrinkle and firming effects gently and efficiently, the present application provides a Ganoderma lucidum and ginseng plant extract composition having anti-wrinkle and firming effects and its application.

[0006] In the first aspect, the present application provides a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, using the following technical solution: A ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects comprises the following components by mass percentage: 15-20% ginseng root extract, 3-7% roxburghii fruit extract, 6-12% dendrobium officinale stem extract, 3-7% matsutake mushroom extract, 30-35% ganoderma lucidum yeast fermentation filtrate, solvent, and 0.1-1% preservative.

[0007] Preferably, the following components are included in percentage by mass: 16.5-18% ginseng root extract, 4.5-6% roxburghii fruit extract, 8-10% dendrobium officinale stem extract, 5-7% matsutake mushroom extract, 33-35% ganoderma lucidum yeast fermentation filtrate, solvent, and 0.1-1% preservative.

[0008] By adopting the above technical scheme, with the cooperation of ginseng root extract, roxburghii fruit extract, dendrobium officinale stem extract, matsutake mushroom extract and ganoderma lucidum yeast fermentation filtrate, a powerful antioxidant, anti-wrinkle and whitening system is jointly constructed, which brings good nourishment and care to the skin.

[0009] Ginseng root extract, Rosa roxburghii fruit extract, Dendrobium officinale stem extract, Tricholoma matsutake extract, and Ganoderma lucidum yeast fermentation filtrate are all derived from natural plants or microbial fermentation products. Therefore, they are highly biocompatible and safe with the skin, can act gently on the skin, reduce the risk of irritation and allergic reactions, and are more suitable for sensitive skin and long-term use.

[0010] The cell regeneration and repairing ability of ginseng root extract complements the antioxidant and whitening effects of roxburghii fruit extract, which together enhance the skin's antioxidant capacity, effectively resist the invasion of free radicals, and reduce the formation of wrinkles and spots. At the same time, the moisturizing and hydrating effect of Dendrobium officinale stem extract provides sufficient moisture and nutrition for the skin, making it more delicate and smooth. The polysaccharides and phenolic components in Matsutake mushroom extract, combined with the amino acids, nucleotides and other active substances in Ganoderma lucidum yeast fermentation filtrate, not only significantly inhibit the production of melanin, reduce spots and dullness, but also further enhance the brightness and luster of the skin. The deep nourishing and regulating function of Ganoderma lucidum yeast fermentation filtrate keeps the skin in a water-oil balance, presenting a healthy and youthful state.

[0011] The mixed system provided in this application has significant anti-wrinkle, anti-oxidation and whitening effects, which are far beyond the reach of a single ingredient. It can solve the problems of skin oxidation, aging and dullness, and can also provide comprehensive nourishment and protection for the skin, making the skin glow with natural brilliance and vitality. This synergistic effect not only enhances the efficacy of each ingredient, but also makes them produce a more lasting and stable effect on the skin.

[0012] In a second aspect, the present application provides a method for preparing a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, using the following technical solution: A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects comprises the following steps: Step 1: Preparation of composite extract: Step 1a: crushing ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom to obtain plant mixed powder; Step 1b: Mix the plant mixed powder and water in a solid-liquid ratio of 1:(12-17), then add 0.5-1wt% of complex enzyme, extract with hot water at 55-65°C, and collect the extract; Step 1c: concentrating the extract under reduced pressure, then adding a solvent to redissolve it, filtering it, and obtaining a composite extract; Step 2: Preparation of Lingzhi Yeast Fermentation Filtrate: Step 2a: Mix the yeast fermentation medium and the crushed red ganoderma lucidum in a ratio of (10-20):1, and sterilize; Step 2b: inoculate 0.01-0.05% yeast and ferment at 25-30°C and 150-170rpm for 16h-18h; Step 2c: After the fermentation is completed, sterilization is performed to terminate the fermentation, and then centrifugation is performed to obtain the supernatant, and filtration is performed to obtain the Ganoderma lucidum yeast fermentation filtrate; Step 3: Mix the composite extract, the Ganoderma lucidum yeast fermentation filtrate, the preservative and the solvent until uniform, to obtain a Ganoderma lucidum and ginseng plant extract composition.

[0013] By adopting the above technical scheme, ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom are extracted by hot water, and the active ingredients in the plants are fully released under the action of suitable temperature and compound enzymes. The compound extract is rich in various active ingredients, such as ginsenosides, vitamin C and antioxidant enzymes in roxburghii fruit, polysaccharides and amino acids in dendrobium officinale, and phenolic compounds in matsutake mushroom, which lay a solid foundation for the subsequent anti-wrinkle and antioxidant effects.

[0014] Red Ganoderma is prepared by microbial fermentation technology, and the fermentation conditions, such as temperature, rotation speed and time, are further limited to allow the red Ganoderma to fully cooperate with yeast. The cell wall of Ganoderma is effectively destroyed, releasing more active ingredients, and yeast also metabolizes to produce a variety of beneficial substances, such as amino acids and nucleotides, which further enhance the health benefits of Ganoderma.

[0015] The compound extract and the fermented filtrate of Ganoderma lucidum yeast are prepared under different preparation conditions and methods, so that the Ganoderma lucidum and ginseng plant extract composition contains a variety of nutritious active ingredients, which can fully exert the anti-wrinkle, anti-oxidation and whitening effects on the skin. It can not only deeply nourish and promote the regeneration and repair of skin cells, but also effectively remove free radicals, reduce oxidative damage, inhibit the production of melanin, brighten the skin tone, make the skin glow with natural radiance, and keep it young and energetic.

[0016] Preferably, the complex enzyme includes a mixture of one or more of pectinase, cellulase and amylase.

[0017] Preferably, the complex enzyme is pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1:(0.8-1.2):(0.8-1.2).

[0018] By adopting the above technical scheme, the compound enzyme is further limited to pectinase, cellulase, amylase and weight ratio, which helps to more fully decompose pectin, cellulose and starch in plant cell walls, thereby accelerating the release of active ingredients in ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom, improving extraction efficiency and enriching effective ingredients in the compound extract. The synergistic effect of pectinase, cellulase and amylase ensures the high efficiency of the extraction process and the purity of the product, and avoids the generation of impurities. At the same time, the action conditions of these enzymes are mild, which is conducive to maintaining the integrity and stability of the active ingredients in the extract.

[0019] Preferably, in step 1b, hot water extraction is performed at least twice.

[0020] Preferably, in step 1a, ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom are crushed and passed through a 50-150 mesh sieve to obtain a plant mixed powder.

[0021] Preferably, the yeast fermentation medium comprises 0.5-2% glucose, 0.1-1% peptone, 0.1-0.5% yeast extract, 0.01-0.03% citric acid, and 0.05-0.2% sodium citrate.

[0022] By adopting the above technical scheme, the composition of the yeast fermentation medium is further limited, which helps to optimize the fermentation environment of the yeast. The rapid growth and reproduction of the yeast also promotes the production and accumulation of the active ingredients of red Ganoderma lucidum, making the content of active ingredients in the fermentation filtrate higher, thereby improving the performance of the Ganoderma lucidum and ginseng plant extract composition.

[0023] In a third aspect, the present application provides an application of a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects in cosmetics, using the following technical solution: The invention discloses an application of a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects in cosmetics. The addition amount of the ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects in cosmetic products ranges from 0.1 to 10%.

[0024] In summary, this application has the following beneficial effects: 1. Since the present application uses ginseng root, roxburghii fruit, dendrobium officinale stem, matsutake mushroom, and red ganoderma lucidum, all of which are derived from nature, after special preparation and extraction, the obtained composition has high biocompatibility and safety with the skin. These ingredients act gently on the skin, reducing the irritation and allergic reactions that may be caused by chemical synthetic substances, and are especially suitable for sensitive skin and long-term use. They not only provide the necessary nutrition and nourishment for the skin, but also reduce the discomfort during use, ensuring the safety of the skin care process.

[0025] 2. The mixed system provided by the present application gives the skin significant anti-wrinkle, anti-oxidation and whitening effects, which are far beyond the reach of a single ingredient. It can not only solve the skin's oxidation, aging and dullness problems, but also provide comprehensive nourishment and protection for the skin, making the skin glow with natural brilliance and vitality. This comprehensive skin care effect is due to the synergistic effect between the ingredients and their ability to deeply nourish and regulate the skin. Long-term use of this skin care system can keep the skin's water and oil balance and present a good appearance. DETAILED DESCRIPTION

[0026] The present application is further described in detail below with reference to the embodiments.

[0027] The raw materials used in the following examples and comparative examples are all commercially available products.

[0028] The following are the sources of some raw materials: Ginseng root was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0029] Rosa roxburghii fruit was purchased from Guizhou Chunye Xiyu Agricultural Products Co., Ltd.

[0030] Dendrobium officinale stems were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0031] Tricholoma matsutake was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0032] Ganoderma lucidum was purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0033] Preparation Example Preparation Example 1 Yeast fermentation medium, glucose 1%, peptone 0.5%, yeast extract 0.3%, citric acid 0.0135%, sodium citrate 0.126%.

[0034] Mix all ingredients, put into containers, seal, and sterilize at 115℃ for 25min.

[0035] Preparation Example 2 Yeast fermentation medium, glucose 0.5%, peptone 1%, yeast extract 0.1%, citric acid 0.01%, sodium citrate 0.2%.

[0036] Mix all ingredients, put into containers, seal, and sterilize at 115℃ for 25min.

[0037] Preparation Example 3 Yeast fermentation medium, glucose 2%, peptone 0.1%, yeast extract 0.5%, citric acid 0.03%, sodium citrate 0.05%.

[0038] Mix all ingredients, put into containers, seal, and sterilize at 115℃ for 25min. Example

[0039] Example 1 This embodiment provides a method for preparing a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, comprising the following steps: Step 1: Preparation of composite extract: Step 1a: Use a grinder to grind ginseng root, roxburghii fruit, dendrobium officinale, and matsutake mushroom, and pass through a 100-mesh sieve to obtain a plant mixed powder.

[0040] Step 1b: The plant mixed powder and water are mixed at a solid-liquid ratio of 1:15 (g:g), and then 0.8 wt% of the complex enzyme is added, and hot water extraction is performed twice at 60° C., and the extracts are collected and combined.

[0041] The complex enzyme includes pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1:1:1.

[0042] Step 1c: Concentrate the extract under reduced pressure to 1 / 4 of the original volume.

[0043] Then, a solvent (butylene glycol) is added to redissolve the mixture, and the mixture is filtered to obtain a composite extract.

[0044] After the above treatment, the ginseng root, roxburghii fruit, dendrobium candidum and matsutake mushroom exist in the composite extract in the form of plant extracts.

[0045] Step 2: Preparation of Lingzhi Yeast Fermentation Filtrate: Step 2a: Use a grinder to grind the red Ganoderma lucidum and pass it through a 100-mesh sieve.

[0046] The yeast fermentation medium of Preparation Example 1 and the crushed red Ganoderma lucidum were mixed in a ratio of 15:1, and sterilized in an autoclave at 121°C for 20 minutes.

[0047] Step 2b: Inoculate 0.02% yeast and ferment in a constant temperature shaking incubator at 28° C. and 160 rpm for 17 h.

[0048] Step 2c: After the fermentation is completed, the fermentation is terminated by sterilizing in an autoclave at 121°C for 20 minutes. After returning to room temperature, the final fermentation product is centrifuged at 4000r / min for 30 minutes, and the supernatant is taken and microfiltered through a membrane to obtain the Ganoderma lucidum yeast fermentation filtrate.

[0049] Step 3: Mix the composite extract, the Ganoderma lucidum yeast fermentation filtrate, the preservative and the solvent until uniform, to obtain a Ganoderma lucidum and ginseng plant extract composition.

[0050] The preservatives are 1,2-hexanediol and p-hydroxyacetophenone.

[0051] The solvent is butanediol.

[0052] The content of each component of the prepared Ganoderma lucidum and ginseng plant extract composition according to mass percentage is shown in Table 1.

[0053] Example 2 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that some parameters are different, specifically comprising the following steps: Step 1: Preparation of composite extract: Step 1a: Use a grinder to grind ginseng root, roxburghii fruit, dendrobium officinale, and matsutake mushroom, and pass through a 50-mesh sieve to obtain a plant mixed powder.

[0054] Step 1b: The plant mixed powder and water are mixed in a solid-liquid ratio of 1:12 (g:g), and then 0.5 wt% of the complex enzyme is added, and hot water extraction is performed twice at 65° C., and the extracts are collected and combined.

[0055] The complex enzyme is pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1:0.8:1.2.

[0056] Step 1c: Concentrate the extract under reduced pressure to 1 / 4 of the original volume.

[0057] Then, a solvent (butylene glycol) is added to redissolve the mixture, and the mixture is filtered to obtain a composite extract.

[0058] After the above treatment, the ginseng root, roxburghii fruit, dendrobium candidum and matsutake mushroom exist in the composite extract in the form of plant extracts.

[0059] Step 2: Preparation of Lingzhi Yeast Fermentation Filtrate: Step 2a: Use a grinder to grind the red Ganoderma lucidum and pass it through a 50-mesh sieve.

[0060] The yeast fermentation medium of Preparation Example 2 and the crushed red ganoderma lucidum were mixed in a ratio of 10:1, and sterilized in an autoclave at 121°C for 20 minutes.

[0061] Step 2b: Inoculate 0.01% yeast and ferment in a constant temperature shaking incubator at 25° C. and 150 rpm for 18 h.

[0062] Step 2c: After the fermentation is completed, the fermentation is terminated by sterilizing in an autoclave at 121°C for 20 minutes. After returning to room temperature, the final fermentation product is centrifuged at 4000r / min for 30 minutes, and the supernatant is taken and microfiltered through a membrane to obtain the Ganoderma lucidum yeast fermentation filtrate.

[0063] Step 3: Mix the composite extract, the Ganoderma lucidum yeast fermentation filtrate, the preservative and the solvent until uniform, to obtain a Ganoderma lucidum and ginseng plant extract composition.

[0064] The content of each component of the prepared Ganoderma lucidum and ginseng plant extract composition according to mass percentage is shown in Table 1.

[0065] Example 3 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that some parameters are different, specifically comprising the following steps: Step 1: Preparation of composite extract: Step 1a: Use a grinder to grind ginseng root, roxburghii fruit, dendrobium officinale, and matsutake mushroom, and pass through a 150-mesh sieve to obtain a plant mixed powder.

[0066] Step 1b: The plant mixed powder and water are mixed at a solid-liquid ratio of 1:17 (g:g), and then 1 wt% of the complex enzyme is added, and hot water extraction is performed twice at 55° C., and the extracts are collected and combined.

[0067] The complex enzyme is pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1:1.2:0.8.

[0068] Step 1c: Concentrate the extract under reduced pressure to 1 / 5 of the original volume.

[0069] Then, a solvent (butylene glycol) is added to redissolve the mixture, and the mixture is filtered to obtain a composite extract.

[0070] After the above treatment, the ginseng root, roxburghii fruit, dendrobium candidum and matsutake mushroom exist in the composite extract in the form of plant extracts.

[0071] Step 2: Preparation of Lingzhi Yeast Fermentation Filtrate: Step 2a: Use a grinder to grind the red Ganoderma lucidum and pass it through a 150-mesh sieve.

[0072] The yeast fermentation medium of Preparation Example 3 and the crushed red Ganoderma lucidum were mixed in a ratio of 20:1, and sterilized in an autoclave at 121°C for 20 minutes.

[0073] Step 2b: Inoculate 0.05% yeast and ferment in a constant temperature shaking incubator at 30° C. and 170 rpm for 16 h.

[0074] Step 2c: After the fermentation is completed, the fermentation is terminated by sterilizing in an autoclave at 121°C for 20 minutes. After returning to room temperature, the final fermentation product is centrifuged at 4000r / min for 30 minutes, and the supernatant is taken and microfiltered through a membrane to obtain the Ganoderma lucidum yeast fermentation filtrate.

[0075] Step 3: Mix the composite extract, the Ganoderma lucidum yeast fermentation filtrate, the preservative and the solvent until uniform, to obtain a Ganoderma lucidum and ginseng plant extract composition.

[0076] The content of each component of the prepared Ganoderma lucidum and ginseng plant extract composition according to mass percentage is shown in Table 1.

[0077] Example 4 A ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that the content of each component is as shown in Table 1.

[0078] Table 1 Example 5 A method for preparing a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that, step 1b: the plant mixed powder and water are mixed in a solid-liquid ratio of 1:15 (g:g), and then 0.8wt% pectinase is added, and hot water extraction is performed twice at 60°C, and the extracts are collected and combined.

[0079] Comparative Example Comparative Example 1 A method for preparing a plant extract composition is different from that of Example 1 in that ginseng root is replaced with scutellaria baicalensis.

[0080] Scutellaria baicalensis is commercially available.

[0081] That is, the ginseng root extract corresponds to the scutellaria baicalensis root extract.

[0082] Comparative Example 2 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that the roxburghii fruit is replaced by wild hawthorn fruit.

[0083] Wild hawthorn fruit is commercially available.

[0084] That is, the roxburghii fruit extract corresponds to the wild hawthorn (CRATAEGUS CUNEATA) fruit extract.

[0085] Comparative Example 3 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that the stems of Dendrobium officinale are replaced with stems of Bletilla striata.

[0086] Bletilla striata stems are commercially available.

[0087] That is, the extract of Dendrobium officinale stem corresponds to the extract of Bletilla striata stem.

[0088] Comparative Example 4 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that Tricholoma matsutake is replaced by Tremella fuciformis.

[0089] Tremella fuciformis is commercially available.

[0090] That is, the extract of Tricholoma matsutake corresponds to the extract of Tremella fuciformis.

[0091] Comparative Example 5 A method for preparing a plant extract composition is different from that of Example 1 in that red Ganoderma lucidum is replaced with grape seeds.

[0092] Grape seeds are commercially available.

[0093] That is, the fermentation filtrate of Ganoderma lucidum yeast is correspondingly changed into the fermentation filtrate of grape seed yeast.

[0094] Comparative Example 6 A method for preparing a ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, which is different from Example 1 in that it comprises the following steps: Step 1: Prepare ginseng root extract, roxburghii fruit extract, dendrobium officinale stem extract, and matsutake mushroom extract respectively: The following takes the preparation of ginseng root extract as an example. When preparing roxburghii fruit extract, dendrobium officinale stem extract, and matsutake mushroom extract, except for the original plants, the remaining steps are the same as those for preparing ginseng root extract.

[0095] Step 1a: Grind the ginseng root using a grinder and pass it through a 100-mesh sieve to obtain ginseng root powder.

[0096] Step 1b: Ginseng root powder and water are mixed in a solid-liquid ratio of 1:15 (g:g), and then 0.8 wt% of complex enzyme is added, and hot water extraction is performed twice at 60° C., and the extracts are collected and combined.

[0097] The complex enzyme includes pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1:1:1.

[0098] Step 1c: Concentrate the extract under reduced pressure to 1 / 4 of the original volume.

[0099] Then, a solvent (butylene glycol) is added to re-dissolve the mixture, and the mixture is filtered to obtain a ginseng root extract.

[0100] Step 2: Preparation of Lingzhi Yeast Fermentation Filtrate: Step 2a: Use a grinder to grind the red Ganoderma lucidum and pass it through a 100-mesh sieve.

[0101] The yeast fermentation medium of Preparation Example 1 and the crushed red Ganoderma lucidum were mixed in a ratio of 15:1, and sterilized in an autoclave at 121°C for 20 minutes.

[0102] Step 2b: Inoculate 0.02% yeast and ferment in a constant temperature shaking incubator at 28° C. and 160 rpm for 17 h.

[0103] Step 2c: After the fermentation is completed, the fermentation is terminated by sterilizing in an autoclave at 121°C for 20 minutes. After returning to room temperature, the final fermentation product is centrifuged at 4000r / min for 30 minutes, and the supernatant is taken and microfiltered through a membrane to obtain the Ganoderma lucidum yeast fermentation filtrate.

[0104] Step 3: Mix the ginseng root extract, the roxburghii fruit extract, the dendrobium officinale stem extract, the matsutake mushroom extract, the ganoderma lucidum yeast fermentation filtrate, the preservative, and the solvent until uniform, to obtain a ganoderma lucidum ginseng plant extract composition.

[0105] Application Examples Application Example 1 A lotion comprises, by mass percentage, 1.6% of cetearyl olivate, 0.4% of sorbitan olivate, 0.64% of C14-22 alcohol, 0.16% of C12-20 alkyl glucoside, 3% of isononyl isononanoate, 2% of dimethicone, 1% of cetearyl alcohol, 2% of cetyl ethylhexanoate, 3% of ethylhexyl palmitate, 0.18% of carbomer, 0.08% of xanthan gum, 0.02% of hyaluronic acid, 5% of glycerol, 3% of 1,3-butylene glycol, 0.05% of ethylhexylglycerol, 0.05% of caprylhydroxamic acid, 0.6% of 1,2-hexanediol, 0.15% of L-arginine, and 5% of a ganoderma lucidum and ginseng plant extract composition having anti-wrinkle and firming effects, and deionized water is added to 100%.

[0106] The Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects is prepared using the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Example 1.

[0107] The preparation method of the above emulsion comprises the following steps: Preparatory phase 1: Take a small amount of deionized water and dissolve L-arginine evenly for later use.

[0108] Pre-prepared phase 2: Take 1% 1,3-butanediol, ethylhexylglycerol, 1,2-hexanediol, and caprylhydroxamic acid, stir and dissolve completely in a water bath at 70°C for later use.

[0109] Add deionized water to a beaker, add carbomer, xanthan gum, hyaluronic acid, glycerin and 2% 1,3-butylene glycol in turn, heat to 82°C, keep warm and stir at 9000rpm / 15min until uniform; take another beaker and add cetearyl olive oil ester, sorbitan olive oil ester, C14-22 alcohol, C12-20 alkyl glucoside, isononyl isononanoate, polydimethylsiloxane, cetearyl alcohol, cetyl ethylhexanoate, ethylhexyl palmitate in turn, heat to 80°C, stir and dissolve completely. Add the oil phase to the water phase and homogenize at 2800rpm for 4min to emulsify, cool down after emulsification, wait until the material body cools down to 45°C, add prefabricated phase 1, prefabricated phase 2, and the ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects in turn, stir evenly to obtain an emulsion.

[0110] Application Example 2 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Example 2.

[0111] Application Example 3 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Example 3.

[0112] Application Example 4 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Example 4.

[0113] Application Example 5 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Example 5.

[0114] Application Example 6 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 1.

[0115] Application Example 7 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 2.

[0116] Application Example 8 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 3.

[0117] Application Example 9 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 4.

[0118] Application Example 10 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 5.

[0119] Application Example 11 An emulsion, which is different from Application Example 1 in that the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects adopts the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects prepared in Comparative Example 6.

[0120] Performance testing 1. Performance testing of plant extract compositions: 1. Antioxidant: Weigh 0.5 g of the plant extract composition of Examples 1-5 and Comparative Examples 1-6 respectively, dissolve them in pure water and transfer them to a 100 mL volumetric flask to make up to volume, and prepare a 5.00×10 -3 g / mL of sample solution.

[0121] Pipette 3 mL of the sample solution to be tested into a 10 mL volumetric flask, then add 3 mL of a 2×10 -4 g / mL DPPH solution. Cover the stopper, shake well, react for 30 minutes, and measure the absorbance A at 517nm i ; Determine the absorbance A of 3mL sample solution and 3mL ethanol mixture j ; The concentration of 3mL was determined to be 2×10 -4 The absorbance A0 after mixing 30 g / mL DPPH solution with 3mL pure water.

[0122] According to the formula P = [1-(A i -A j ) / A0]×100%, and the measured absorbance is substituted into the inhibitory rate of the test solution on DPPH· free radicals.

[0123] The inhibition rates are recorded in Table 2.

[0124] 2. Anti-wrinkle: The plant extract compositions of Examples 1-5 and Comparative Examples 1-6 were respectively dissolved in DMSO to prepare 10 mg / mL test sample solutions for later use.

[0125] Weigh 10 mg of elastase, add 100 ml of borate buffer with a pH of 8.4 to prepare an elastase solution for later use.

[0126] Take 20 mg of Congo red elastin and add 10 ml of borate buffer with a pH of 8.4 to prepare a substrate solution for use.

[0127] Take 0.1mL Tris buffer, 50μL elastase solution and 50μL sample solution to be tested in a 96-well plate, place it at 25℃ for pre-incubation for 20min; add 50μL substrate solution; continue to incubate at 25℃ for 60min, and measure the absorbance at 495nm with an enzyme marker to obtain the absorbance value T of the sample well.

[0128] Take 0.1mL of Tris buffer, 50μL of elastase solution and 50μL of solvent DMSO (without the sample to be tested) in a 96-well plate and pre-incubate at 25℃ for 20min; add 50μL of substrate solution; continue to incubate at 25℃ for 60min, and measure the absorbance at 495nm with an enzyme reader to obtain the absorbance value C of the control well.

[0129] Finally, the elastase inhibition rate was calculated according to the following formula and recorded in Table 2.

[0130] Elastase inhibition rate (%) = [1-(sample well absorbance value T / control well absorbance value C)] × 100% Table 2 Group DPPH free radical inhibition rate (%) Elastase inhibition rate (%) Example 1 78.27 81.29 Example 2 67.75 67.64 Example 3 70.32 69.99 Example 4 75.25 71.38 Example 5 63.60 65.22 Comparative Example 1 50.62 52.84 Comparative Example 2 48.50 49.66 Comparative Example 3 48.96 49.00 Comparative Example 4 46.28 48.95 Comparative Example 5 51.04 50.83 Comparative Example 6 55.84 58.35 According to the test data in Table 2, the plant extract compositions of Examples 1-5 exhibit excellent anti-wrinkle and anti-oxidation effects. However, Comparative Examples 1-6, based on Example 1, have different degrees of damage to the specific scheme, resulting in a significant decrease in performance and failure to exert a specific synergistic effect.

[0131] 2. Emulsion performance test: 1. Whitening: 110 female volunteers aged 25-35 were selected and randomly divided into 11 groups, with 10 people in each group. One group corresponds to one of the lotions in Application Examples 1-11.

[0132] Use clean water to cleanse the face and spray toner. After complete absorption, use a skin colorimeter to measure the ITA° value of the volunteer's face and record it in the book.

[0133] Then take 2 mL of the lotion and apply it evenly on the face, once in the morning and once in the evening, for 28 days.

[0134] At 28 days, the ITA° values ​​of the volunteers' faces were measured again using a skin colorimeter.

[0135] Calculate the whitening improvement rate according to the following formula and record the results in Table 3.

[0136] Table 3 Group Whitening improvement rate (%) Application Example 1 12.5 Application Example 2 11.2 Application Example 3 13.3 Application Example 4 12.7 Application Example 5 9.4 Application Example 6 5.0 Application Example 7 5.2 Application Example 8 4.9 Application Example 9 4.7 Application Example 10 5.0 Application Example 11 6.1 According to the test data in Table 3, the emulsions using the plant extract composition specially prepared in this application (Application Examples 1-5) can show excellent whitening effects. Although the emulsions of Application Examples 6-11 also have certain whitening and enhancement effects, they are not as significant as those of Application Examples 1-5.

[0137] According to the survey of volunteers, the emulsions of Application Examples 1-11 did not cause any discomfort to the volunteers and were very mild.

[0138] 2. Referring to the above-mentioned anti-oxidation and anti-wrinkle test method, the emulsion of Example 1 was tested and the results were recorded in Table 4.

[0139] Table 4 Group DPPH free radical inhibition rate (%) Elastase inhibition rate (%) Application Example 1 75.27 72.8 3. Stability: A. Centrifugal stability: The emulsion of Application Example 1 was centrifuged at 3000 r / min for 30 min and observed.

[0140] Result: No abnormal phenomenon occurred, indicating good centrifugal stability.

[0141] B. Heat and cold resistance stability: The emulsion of Application Example 1 was placed in a heat (45°C, 48°C) and cold (-15°C, 5°C) cycle for 4 weeks and then returned to room temperature for observation.

[0142] Result: No precipitation or discoloration occurred, indicating good heat and cold resistance stability.

[0143] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects, characterized in that: The invention comprises the following components in percentage by mass: 15-20% of ginseng root extract, 3-7% of roxburghii fruit extract, 6-12% of dendrobium officinale stem extract, 3-7% of matsutake mushroom extract, 30-35% of ganoderma lucidum yeast fermentation filtrate, solvent and 0.1-1% of preservative.

2. The Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 1, characterized in that: The invention comprises the following components in percentage by mass: 16.5-18% of ginseng root extract, 4.5-6% of roxburghii fruit extract, 8-10% of dendrobium officinale stem extract, 5-7% of matsutake mushroom extract, 33-35% of ganoderma lucidum yeast fermentation filtrate, solvent and 0.1-1% of preservative.

3. A method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to any one of claims 1 to 2, characterized in that: The following steps are involved: Step 1: Preparation of composite extract: Step 1a: crushing ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom to obtain plant mixed powder; Step 1b: the plant mixed powder is mixed with water in a ratio of 1:(12-17) and then 0.5-1wt% of the complex enzyme is added, hot water extraction is performed at 55-65°C and the extract is collected; Step 1c: Concentrate the extract under reduced pressure, then add a solvent to redissolve it, and filter it to obtain a composite extract; Step 2: Preparation of Ganoderma lucidum yeast fermentation filtrate: Step 2a: Mix the yeast fermentation medium and the crushed red ganoderma lucidum in a ratio of (10-20):1, and sterilize; Step 2b: Inoculate 0.01-0.05% yeast and ferment at 25-30°C and 150-170rpm for 16h-18h; Step 2c: After the fermentation is completed, sterilization is performed to terminate the fermentation, and then centrifugation is performed to obtain the supernatant, and filtration is performed to obtain the Ganoderma lucidum yeast fermentation filtrate; Step 3: Mix the composite extract, the Ganoderma lucidum yeast fermentation filtrate, the preservative and the solvent until uniform, to obtain a Ganoderma lucidum and ginseng plant extract composition.

4. The method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 3, characterized in that: The complex enzyme comprises a mixture of one or more of pectinase, cellulase and amylase.

5. The method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 4, characterized in that: The complex enzyme is pectinase, cellulase and amylase, and the weight ratio of pectinase, cellulase and amylase is 1: (0.8-1.2): (0.8-1.2).

6. The method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 3, characterized in that: In the step 1b, hot water is used for extraction at least twice.

7. The method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 3, characterized in that: In the step 1a, ginseng root, roxburghii fruit, dendrobium officinale and matsutake mushroom are crushed and passed through a 50-150 mesh sieve to obtain a plant mixed powder.

8. The method for preparing the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects according to claim 3, characterized in that: The yeast fermentation medium comprises 0.5-2% glucose, 0.1-1% peptone, 0.1-0.5% yeast extract, 0.01-0.03% citric acid, and 0.05-0.2% sodium citrate.

9. An application of a Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effect obtained by the preparation method of the Ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effect according to any one of claims 1-2 or any one of claims 3-8 in cosmetics, characterized in that: The amount of the ganoderma lucidum and ginseng plant extract composition with anti-wrinkle and firming effects added to cosmetic products ranges from 0.1 to 10%.

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