Preparation method of efficient antibacterial agent containing cordyceps militaris extract and product

Abstract

The invention relates to the technical field of antibacterial agents, and discloses a preparation method and a product of an efficient antibacterial agent containing a cordyceps militaris extract, and the preparation method comprises the following steps: a cordyceps militaris pretreatment step: ultrasonic treatment and reflux extraction; an extract separation and purification step: centrifuging to take supernate, adsorbing with a macroporous resin column, and eluting; preparing an antibacterial agent; and concentrating under reduced pressure. The antibacterial agent is light yellow transparent liquid, has a pH value of 6-7, and is free of peculiar smell and stable in appearance and property. The minimum inhibitory concentrations (MIC) of the antibacterial agent to escherichia coli, staphylococcus aureus and candida albicans are 0.3 to 0.5 mg / mL, 0.2 to 0.4 mg / mL and 0.3 to 0.6 mg / mL respectively, and the antibacterial agent shows excellent antibacterial performance; after the antibacterial agent is stored for 18 months at normal temperature, the antibacterial activity retention rate is 90% or above, and the antibacterial agent has good stability.

Description

Technical Field

[0001] The invention relates to the technical field of antimicrobial agents, and in particular to a preparation method and a product of a high-efficiency antimicrobial agent containing a Cordyceps militaris extract. Background Art

[0002] In today's society, antimicrobial agents play a vital role in many fields. With the continuous improvement of people's requirements for health and hygiene, as well as the rapid development of industries such as food, medicine, and daily chemicals, the demand for efficient and safe antimicrobial agents is becoming increasingly urgent.

[0003] In the medical field, various wound infections and disease transmission issues have prompted people to seek better topical antibacterial preparations. Although traditional antibacterial drugs can inhibit bacterial growth to a certain extent, they have drug resistance problems and may have side effects on normal human tissues. Moreover, for some special cases such as chronic wounds, burns and scalds, antibacterial agents are required to have better functions such as promoting healing and reducing inflammation.

[0004] The food industry faces huge challenges in food preservation. Microbial contamination is one of the main causes of food spoilage and corruption, which not only causes huge economic losses, but also threatens the health of consumers. Traditional food preservation methods such as refrigeration and the addition of chemical preservatives have limitations. Refrigeration requires continuous cold chain support and is costly; some chemical preservatives may have safety risks, causing consumers to worry about food safety. Therefore, the development of natural, efficient and safe food preservation antimicrobial agents has become a research hotspot in the food industry.

[0005] In the field of daily chemicals, skin problems such as acne, blackheads, and skin allergies are closely related to microbial infections. Consumers are increasingly demanding skin care products with antibacterial functions, and they also require these products to be mild and non-irritating to the skin and have other beneficial functions such as moisturizing. However, some existing antibacterial skin care products may have problems such as unsatisfactory antibacterial effects, high skin irritation, or damage to the skin barrier.

[0006] As a traditional medicinal fungus, Cordyceps militaris contains a variety of bioactive ingredients, such as cordycepin, polysaccharides, etc., and has multiple functions such as antibacterial, antioxidant, and immunomodulatory. In recent years, research on the antibacterial properties of Cordyceps militaris extracts has gradually attracted attention. However, the current preparation method of Cordyceps militaris extract antibacterial agents is not perfect enough, and there are problems such as low extraction efficiency, low extract purity, and poor stability of antibacterial agents. For example, during the extraction process, due to the rough process, the effective ingredients are lost or the extraction is incomplete; during the preparation of antibacterial agents, there is no suitable formula and process to ensure its stability and effectiveness in different application scenarios. Therefore, a new and more optimized method for preparing an efficient antibacterial agent containing Cordyceps militaris extract is needed to give full play to the antibacterial potential of Cordyceps militaris and meet the strict requirements of antibacterial agents in the fields of medicine, food, and daily chemicals. Summary of the invention

[0007] 1. Technical issues to be solved

[0008] In view of the deficiencies in the prior art, the present invention provides a method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract and a product thereof.

[0009] (II) Technical solution

[0010] A method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract comprises the following steps:

[0011] S1. Cordyceps militaris pretreatment steps:

[0012] Select Cordyceps militaris fruiting bodies that are free of mildew, insect infestation and good in dryness, use ultrafine grinding technology to grind them into a particle size of 20-50 meshes, add an extraction solvent thereto according to a solid-liquid ratio of 1:10-20 (g / mL), wherein the extraction solvent is a specially treated ethanol aqueous solution with a volume fraction of 60-80%, and the ethanol aqueous solution needs to be filtered and sterilized through a 0.22 μm microporous filter membrane before use, and ultrasonically treat the mixture at 30-50° C. with an ultrasonic power of 200-400 W for 20-40 minutes, wherein pulsed ultrasound is used during the ultrasonic treatment, and the ratio of ultrasonic working time to intermittent time is 1:1;

[0013] The mixture after ultrasonic treatment is subjected to reflux extraction at 40-60°C for 2-4h. The reflux device uses a glass instrument with a condenser. The condenser is water-cooled and the water temperature is precisely controlled at 5-15°C to ensure a good condensation effect. During the reflux extraction process, the mixed solution is stirred every 30 minutes at a stirring speed of 100-200r / min and a stirring time of 2-3min to allow the material to fully contact the solvent.

[0014] S2. Extract separation and purification steps:

[0015] The mixed solution after reflux extraction is centrifuged at 4000-6000 r / min for 10-20 min, with the centrifugal temperature maintained at 4-8°C, and the supernatant is taken;

[0016] The supernatant is adsorbed by a pretreated macroporous resin column, the macroporous resin model is AB-8; the macroporous resin column is firstly immersed in 90-95% ethanol for 24-48 hours for activation before use, and then rinsed with deionized water until the effluent has no alcohol taste, and then balanced with a phosphate buffer solution with a pH value of 6-7 until the pH value of the effluent is consistent with that of the buffer solution; during the adsorption process, the column temperature is controlled at 20-30°C, the flow rate is 2-3BV / h, and BV is the column bed volume; after adsorption, the resin column is firstly rinsed with deionized water, the rinsing volume is 2-3 times the volume of the resin column, the rinsing flow rate is 3-4BV / h, and then eluted with an ethanol aqueous solution with a volume fraction of 30-50%, the elution volume is 4-6 times the volume of the resin column, the elution flow rate is 1-2BV / h, and the eluate is collected;

[0017] S3, antimicrobial agent preparation steps:

[0018] The eluate is concentrated under reduced pressure at 50-60° C. and a vacuum degree of 0.08-0.09 MPa to 1 / 5-1 / 3 of the original volume to obtain a Cordyceps militaris extract concentrate; during the concentration process, a rotary evaporator is used, the rotation speed of the evaporator is 50-80 r / min, and a trace amount of nitrogen is introduced into the surface of the concentrate at a nitrogen flow rate of 5-10 mL / min to prevent oxidation;

[0019] A surfactant is added to the concentrated solution, wherein the surfactant is polysorbate 80 and the added amount is 5-10% of the mass of the concentrated solution; a natural antioxidant is added, wherein the antioxidant is rosemary extract and the added amount is 0.5-1% of the mass of the concentrated solution; the mixture is stirred evenly in a light-proof and oxygen-free environment at a stirring speed of 300-500 r / min and a stirring time of 10-20 min to prepare a high-efficiency antibacterial agent containing Cordyceps militaris extract; and nitrogen is continuously introduced during the stirring process at a nitrogen flow rate of 10-20 mL / min.

[0020] Preferably, in step S1, the crushed Cordyceps militaris fruiting bodies are soaked before ultrasonic treatment for 30-60 minutes in deionized water containing 0.1-0.3% vitamin C to reduce oxidation of Cordyceps militaris during the treatment process.

[0021] Preferably, the macroporous resin column needs to be checked for integrity before use, using a bubble point method to ensure that it has good integrity, no leakage or damage, so as to ensure the adsorption and elution effects.

[0022] Preferably, in step S3, before adding the surfactant and the antioxidant to the concentrated solution, the concentrated solution is first subjected to microfiltration treatment, and the pore size of the microfiltration membrane is 0.1-0.2 μm to remove possible residual impurity particles.

[0023] Preferably, during the entire preparation process, the containers and pipes used are made of food-grade 304 stainless steel or medical-grade polytetrafluoroethylene to avoid chemical reactions with the materials.

[0024] Preferably, the minimum inhibitory concentration (MIC) of the antibacterial agent against Escherichia coli, Staphylococcus aureus, and Candida albicans is 0.3-0.5 mg / mL, 0.2-0.4 mg / mL, and 0.3-0.6 mg / mL, respectively, showing excellent antibacterial properties; after the antibacterial agent is stored at room temperature for 18 months, its antibacterial activity retention rate is above 90%, and it has good stability.

[0025] Preferably, the antibacterial agent is a light yellow transparent liquid with a pH value of 6-7, no odor, and stable appearance and properties.

[0026] Preferably, the antibacterial agent can be applied to external antibacterial preparations in the medical field, food preservatives in the food industry, or antibacterial skin care products in the daily chemical field. When used, it can be diluted to a suitable concentration according to different application scenarios, and has good compatibility with other ingredients in different application scenarios.

[0027] Preferably, when the antibacterial agent is used as a food preservative, the antibacterial film formed on the food surface has a thickness of 0.01-0.03 mm. The antibacterial film has good air permeability and moisture retention, can effectively inhibit the growth of microorganisms, and extend the shelf life of food by 5-7 days without affecting the taste and flavor of the food.

[0028] (III) Beneficial technical effects

[0029] In terms of antibacterial properties, it has excellent inhibitory ability against common pathogens such as Escherichia coli, Staphylococcus aureus, and Candida albicans, and the minimum inhibitory concentration (MIC) is low, indicating that only a small amount of antibacterial agent is needed to effectively inhibit the growth of microorganisms. This makes it effective in preventing and treating wound infections in the medical field, strongly inhibiting microorganisms that cause food spoilage in food preservation, and reducing the number of harmful bacteria on the skin surface in the daily chemical field, thereby reducing the incidence of skin infection-related problems.

[0030] In terms of stability, after the antimicrobial agent is stored at room temperature for 18 months, the antimicrobial activity retention rate is as high as over 90%. This feature enables it to maintain a stable antimicrobial effect regardless of long-term storage or use under different environmental conditions. In the pharmaceutical field, it can ensure that the drug remains effective within its validity period; in food preservation, it can ensure stable protection of food throughout its shelf life; in daily chemical products, it can extend the shelf life of the product.

[0031] In terms of safety, antimicrobial agents are non-irritating to the skin and mucous membranes, meeting hygiene standards and safety requirements. In medical external preparations, they will not cause additional damage to the tissues around the wound; in daily chemical skin care products, they can be safely used for daily skin care without causing adverse reactions such as skin allergies, redness, and swelling.

[0032] In terms of application adaptability, as a food preservative, the antibacterial film formed has an appropriate thickness, good air permeability and moisture retention, and can extend the shelf life of food without affecting the quality of food. In the field of daily chemicals, it has good compatibility with other skin care ingredients and can adapt to a variety of formulas; in the field of medicine, it can synergize with other treatment methods (such as combined use with antibiotics) to enhance the antibacterial effect, showing a wide range of application prospects and huge market value. DETAILED DESCRIPTION

[0033] Example 1

[0034] S1. Cordyceps militaris pretreatment steps:

[0035] Select Cordyceps militaris fruiting bodies that are free of mildew, insect infestation and good dryness, use ultrafine grinding technology to crush them to a particle size of 20 mesh, and soak them for 30 minutes. The soaking liquid is deionized water containing 0.1% vitamin C. Filter and dry, add an extraction solvent according to a solid-liquid ratio of 1:10 (g / mL), the extraction solvent is a specially treated 60% ethanol aqueous solution, and the ethanol aqueous solution needs to be filtered and sterilized by a 0.22μm microporous filter membrane before use, and the mixture is ultrasonically treated for 20 minutes at 30°C with an ultrasonic power of 200W. Pulsed ultrasound is used during the ultrasonic treatment, and the ratio of ultrasonic working time to intermittent time is 1:1;

[0036] The mixture after ultrasonic treatment was refluxed at 40 °C for 2 h. The reflux device was a glass instrument with a condenser. The condenser was water-cooled and the water temperature was precisely controlled at 5 °C to ensure a good condensation effect. During the reflux extraction, the mixed solution was stirred every 30 min at a speed of 100 r / min for 2 min to ensure that the material was fully in contact with the solvent.

[0037] S2. Extract separation and purification steps:

[0038] The mixed solution after reflux extraction was centrifuged at 4000 r / min for 10 min, with the centrifugal temperature maintained at 4°C, and the supernatant was taken;

[0039] The supernatant is adsorbed by a pretreated macroporous resin column, the macroporous resin model is AB-8; the macroporous resin column is first soaked in 90% ethanol for 24 hours for activation before use, and then rinsed with deionized water until the effluent has no alcohol taste, and then balanced with a phosphate buffer solution with a pH value of 6 until the pH value of the effluent is consistent with that of the buffer solution; during the adsorption process, the column temperature is controlled at 20°C, the flow rate is 2BV / h, and BV is the column bed volume; after adsorption, the resin column is first rinsed with deionized water, the rinse volume is twice the volume of the resin column, and the rinse flow rate is 3BV / h, and then eluted with a volume fraction of 30% ethanol aqueous solution, the elution volume is 4 times the volume of the resin column, and the elution flow rate is 1BV / h, and the eluate is collected.

[0040] S3, antimicrobial agent preparation steps:

[0041] The eluate was concentrated under reduced pressure at 50°C and a vacuum degree of 0.08 MPa to 1 / 5 of the original volume to obtain a Cordyceps militaris extract concentrate; during the concentration process, a rotary evaporator was used with a rotation speed of 50 r / min, and a trace amount of nitrogen was introduced into the surface of the concentrate at a nitrogen flow rate of 5 mL / min to prevent oxidation;

[0042] The concentrate was subjected to microfiltration treatment with a microfiltration membrane having a pore size of 0.1 μm. A surfactant was added to the concentrate, wherein the surfactant was polysorbate 80 and the added amount was 5% of the mass of the concentrate. A natural antioxidant was also added, wherein the antioxidant was rosemary extract and the added amount was 0.5% of the mass of the concentrate. The concentrate was stirred evenly in a light-proof and oxygen-free environment at a speed of 300 r / min for 10 min to prepare a high-efficiency antibacterial agent containing Cordyceps militaris extract. Nitrogen was continuously introduced during the stirring process at a nitrogen flow rate of 10 mL / min.

[0043] Example 2

[0044] S1. Cordyceps militaris pretreatment steps:

[0045] Select Cordyceps militaris fruiting bodies that are free of mildew, insect infestation and good dryness, use ultrafine grinding technology to crush them to a particle size of 50 mesh, and soak them for 60 minutes. The soaking liquid is deionized water containing 0.3% vitamin C. Filter and dry, add an extraction solvent according to a solid-liquid ratio of 1:20 (g / mL), the extraction solvent is a specially treated ethanol aqueous solution with a volume fraction of 80%, and the ethanol aqueous solution needs to be filtered and sterilized by a 0.22μm microporous filter membrane before use, and the mixture is ultrasonically treated for 40 minutes at 50°C with an ultrasonic power of 400W. Pulsed ultrasound is used during the ultrasonic treatment, and the ratio of ultrasonic working time to intermittent time is 1:1;

[0046] The mixture after ultrasonic treatment is refluxed and extracted at 40-60°C for 4h. The reflux device uses a glass instrument with a condenser. The condenser is water-cooled and the water temperature is precisely controlled at 15°C to ensure a good condensation effect. During the reflux extraction process, the mixed liquid is stirred every 30min at a speed of 200r / min and a stirring time of 3min to allow the material to fully contact the solvent.

[0047] S2. Extract separation and purification steps:

[0048] The mixed solution after reflux extraction was centrifuged at 6000 r / min for 20 min, with the centrifugal temperature maintained at 8°C, and the supernatant was taken;

[0049] The supernatant is adsorbed by a pretreated macroporous resin column, the macroporous resin model is AB-8; the macroporous resin column is firstly activated by soaking in 95% ethanol for 48 hours before use, and then rinsed with deionized water until the effluent has no alcohol taste, and then balanced with a phosphate buffer solution with a pH value of 7 until the pH value of the effluent is consistent with that of the buffer solution; during the adsorption process, the column temperature is controlled at 30°C, the flow rate is 3BV / h, and BV is the column bed volume; after adsorption, the resin column is first rinsed with deionized water, the rinse volume is 3 times the volume of the resin column, the rinse flow rate is 4BV / h, and then eluted with a volume fraction of 50% ethanol aqueous solution, the elution volume is 6 times the volume of the resin column, the elution flow rate is 2BV / h, and the eluate is collected.

[0050] S3, antimicrobial agent preparation steps:

[0051] The eluate was concentrated under reduced pressure at 60°C and a vacuum degree of 0.09 MPa to 1 / 3 of the original volume to obtain a Cordyceps militaris extract concentrate; during the concentration process, a rotary evaporator was used with a rotation speed of 80 r / min, and a trace amount of nitrogen was introduced into the surface of the concentrate at a nitrogen flow rate of 10 mL / min to prevent oxidation;

[0052] The concentrate was subjected to microfiltration treatment with a microfiltration membrane having a pore size of 0.2 μm. A surfactant was added to the concentrate, wherein the surfactant was polysorbate 80 and the addition amount was 10% of the mass of the concentrate. A natural antioxidant was also added, wherein the antioxidant was rosemary extract and the addition amount was 1% of the mass of the concentrate. The concentrate was stirred evenly in a light-proof and oxygen-free environment at a speed of 500 r / min for 20 min to prepare a high-efficiency antibacterial agent containing Cordyceps militaris extract. Nitrogen was continuously introduced during the stirring process at a nitrogen flow rate of 20 mL / min.

[0053] Example 3

[0054] S1. Cordyceps militaris pretreatment steps:

[0055] Select Cordyceps militaris fruiting bodies that are free of mildew, insect infestation and good dryness, use ultrafine grinding technology to crush them to a particle size of 40 mesh, and soak them for 50 minutes. The soaking liquid is deionized water containing 0.2% vitamin C. Filter and dry, add an extraction solvent according to a solid-liquid ratio of 1:15 (g / mL), the extraction solvent is a specially treated 75% volume fraction ethanol aqueous solution, and the ethanol aqueous solution needs to be filtered and sterilized by a 0.22μm microporous filter membrane before use, and the mixture is ultrasonically treated for 30 minutes at 40°C with an ultrasonic power of 300W. Pulsed ultrasound is used during the ultrasonic treatment, and the ratio of ultrasonic working time to intermittent time is 1:1;

[0056] The mixture after ultrasonic treatment was refluxed at 50°C for 3 h. The reflux device was a glass instrument with a condenser. The condenser was water-cooled and the water temperature was precisely controlled at 10°C to ensure a good condensation effect. During the reflux extraction, the mixed solution was stirred every 30 min at a speed of 150 r / min for 3 min to ensure that the material was fully in contact with the solvent.

[0057] S2. Extract separation and purification steps:

[0058] The mixed solution after reflux extraction was centrifuged at 5000 r / min for 15 min, with the centrifugal temperature maintained at 6°C, and the supernatant was taken;

[0059] The supernatant is adsorbed by a pretreated macroporous resin column, the macroporous resin model is AB-8; the macroporous resin column is firstly activated by soaking in 95% ethanol for 30 hours before use, and then rinsed with deionized water until the effluent has no alcohol taste, and then balanced with a phosphate buffer solution with a pH value of 6 until the pH value of the effluent is consistent with that of the buffer solution; during the adsorption process, the column temperature is controlled at 25°C, the flow rate is 3BV / h, and BV is the column bed volume; after adsorption, the resin column is first rinsed with deionized water, the rinse volume is 3 times the volume of the resin column, and the rinse flow rate is 3BV / h, and then eluted with a volume fraction of 40% ethanol aqueous solution, the elution volume is 5 times the volume of the resin column, and the elution flow rate is 1.5BV / h, and the eluate is collected.

[0060] S3, antimicrobial agent preparation steps:

[0061] The eluate was concentrated under reduced pressure at 60°C and a vacuum degree of 0.08 MPa to 1 / 4 of the original volume to obtain a Cordyceps militaris extract concentrate; during the concentration process, a rotary evaporator was used with a rotation speed of 60 r / min, and a trace amount of nitrogen was introduced into the surface of the concentrate at a nitrogen flow rate of 6 mL / min to prevent oxidation;

[0062] The concentrate was subjected to microfiltration treatment with a microfiltration membrane having a pore size of 0.2 μm. A surfactant was added to the concentrate, wherein the surfactant was polysorbate 80 and the added amount was 8% of the mass of the concentrate. A natural antioxidant was also added, wherein the antioxidant was rosemary extract and the added amount was 0.6% of the mass of the concentrate. The concentrate was stirred evenly in a light-proof and oxygen-free environment at a speed of 400 r / min for 20 min to prepare a high-efficiency antibacterial agent containing Cordyceps militaris extract. Nitrogen was continuously introduced during the stirring process at a nitrogen flow rate of 15 mL / min.

[0063] Comparative Example 1: The filtrate of the Cordyceps militaris and ginseng fermentation product prepared in Example 1 of Chinese Patent CN113584087B.

[0064] Antibacterial performance test: The antibacterial test was carried out according to the evaluation method of standard WST650-2019. The test bacteria were Escherichia coli (8099), Staphylococcus aureus, and Candida albicans. The concentration of bacterial suspension was 1×10 6 CFU / mL, test results are shown in Table 1.

[0065] Table 1

[0066] Group Escherichia coli (8099) / % Staphylococcus aureus / % Candida albicans / % Example 1 82.5 75.6 72.3 Example 2 83.6 72.6 73.6 Example 3 81.5 73.5 72.1 Comparative Example 1 72.3 - -

[0067] The minimum inhibitory concentration (MIC) of the antibacterial agent against Escherichia coli, Staphylococcus aureus and Candida albicans was 0.3-0.5 mg / mL, 0.2-0.4 mg / mL and 0.3-0.6 mg / mL, respectively.

[0068] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract, characterized in that: The following steps are involved: S1. Cordyceps militaris pretreatment steps: Select Cordyceps militaris fruiting bodies that are free of mildew, insect infestation and good in dryness, use ultrafine grinding technology to grind them into a particle size of 20-50 meshes, add an extraction solvent thereto according to a solid-liquid ratio of 1:10-20, wherein the extraction solvent is a specially treated ethanol aqueous solution with a volume fraction of 60-80%, and the ethanol aqueous solution needs to be filtered and sterilized through a 0.22 μm microporous filter membrane before use, and ultrasonically treat the mixture at 30-50° C. with an ultrasonic power of 200-400 W for 20-40 minutes, using pulsed ultrasound during the ultrasonic treatment process, and the ratio of ultrasonic working time to intermittent time is 1:1; The mixture after ultrasonic treatment is subjected to reflux extraction at 40-60°C for 2-4h. The reflux device uses a glass instrument with a condenser. The condenser is water-cooled and the water temperature is precisely controlled at 5-15°C to ensure a good condensation effect. During the reflux extraction process, the mixed solution is stirred every 30 minutes at a stirring speed of 100-200r / min and a stirring time of 2-3min to allow the material to fully contact the solvent. S2. Extract separation and purification steps: The mixed solution after reflux extraction is centrifuged at 4000-6000 r / min for 10-20 min, with the centrifugal temperature maintained at 4-8°C, and the supernatant is taken; The supernatant is adsorbed by a pretreated macroporous resin column, the macroporous resin model is AB-8; the macroporous resin column is firstly immersed in 90-95% ethanol for 24-48 hours for activation before use, and then rinsed with deionized water until the effluent has no alcohol taste, and then balanced with a phosphate buffer solution with a pH value of 6-7 until the pH value of the effluent is consistent with that of the buffer solution; during the adsorption process, the column temperature is controlled at 20-30°C, the flow rate is 2-3BV / h, and BV is the column bed volume; after adsorption, the resin column is firstly rinsed with deionized water, the rinsing volume is 2-3 times the volume of the resin column, the rinsing flow rate is 3-4BV / h, and then eluted with an ethanol aqueous solution with a volume fraction of 30-50%, the elution volume is 4-6 times the volume of the resin column, the elution flow rate is 1-2BV / h, and the eluate is collected; S3, antimicrobial agent preparation steps: The eluate is concentrated under reduced pressure at 50-60° C. and a vacuum degree of 0.08-0.09 MPa to 1 / 5-1 / 3 of the original volume to obtain a Cordyceps militaris extract concentrate; During the concentration process, a rotary evaporator is used with a rotation speed of 50-80r / min. At the same time, a small amount of nitrogen is introduced into the surface of the concentrated liquid with a nitrogen flow rate of 5-10mL / min to prevent oxidation; A surfactant is added to the concentrated solution, wherein the surfactant is polysorbate 80 and the added amount is 5-10% of the mass of the concentrated solution; a natural antioxidant is added, wherein the antioxidant is rosemary extract and the added amount is 0.5-1% of the mass of the concentrated solution; the mixture is stirred evenly in a light-proof and oxygen-free environment at a stirring speed of 300-500 r / min and a stirring time of 10-20 min to prepare a high-efficiency antibacterial agent containing Cordyceps militaris extract; and nitrogen is continuously introduced during the stirring process at a nitrogen flow rate of 10-20 mL / min.

2. The method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract according to claim 1, characterized in that: In step S1, the crushed Cordyceps militaris fruiting bodies are soaked before ultrasonic treatment, the soaking time is 30-60 minutes, and the soaking liquid is deionized water containing 0.1-0.3% vitamin C.

3. The method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract according to claim 1, characterized in that: The integrity of the macroporous resin column must be checked before use using the bubble point method.

4. The method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract according to claim 1, characterized in that: In step S3, before adding the surfactant and the antioxidant to the concentrated solution, the concentrated solution is first subjected to microfiltration treatment, and the pore size of the microfiltration membrane is 0.1-0.2 μm.

5. The method for preparing a highly effective antibacterial agent containing Cordyceps militaris extract according to claim 1, characterized in that: During the entire preparation process, the containers and pipes used are made of food-grade 304 stainless steel or medical-grade polytetrafluoroethylene.

6. A high-efficiency antibacterial agent containing Cordyceps militaris extract obtained by the method for preparing a high-efficiency antibacterial agent containing Cordyceps militaris extract according to any one of claims 1 to 5, characterized in that: The antibacterial agent is a light yellow transparent liquid with a pH value of 6-7, no odor, and stable appearance and properties.

7. The high-efficiency antibacterial agent containing Cordyceps militaris extract according to claim 6, characterized in that: The antibacterial agent can be applied to external antibacterial preparations in the medical field, food preservatives in the food industry, or antibacterial skin care products in the daily chemical field. When used, it can be diluted to a suitable concentration according to different application scenarios, and has good compatibility with other ingredients in different application scenarios.

8. The high-efficiency antibacterial agent containing Cordyceps militaris extract according to claim 6, characterized in that: When the antibacterial agent is used as a food preservative, the antibacterial film formed on the food surface has a thickness of 0.01-0.03 mm. The antibacterial film has good air permeability and moisture retention, can effectively inhibit the growth of microorganisms, and extend the shelf life of food by 5-7 days without affecting the taste and flavor of the food.